KR20190046702A - New Organic nanofluid materials for improving C1 gas transmission efficiency - Google Patents

Abstract

The present invention relates to a nanoparticle based on organic materials for improving a mass transfer efficiency in a bioconversion process of C1 gases such as carbon monoxide (CO) and methane (CH_4), and a nanofluid formed by the nanoparticle. The nanofluid containing a novel organic nanoparticle of the present invention makes a gas having a low water solubility efficiently used in an aqueous solution, thereby being usefully used for microorganisms to obtain a bioconversion product by using gas while a microorganism strain overcomes cytotoxicity of nanoparticles in a bioconversion process. In addition, the novel organic material based nanofluid of the present invention is applied to various cell cultivating media for microorganism conversion and is expected to improve cell growth rates and increase gas consumption by cells, thereby being useful in terms of the industrial applicability.

Description

{New organic nanofluid materials for improving C1 gas transmission efficiency}

The invention nanoparticles comprising chitosan and lipid compounds or nano activated carbon to increase the generation fermentation of water promotes the gas consumption rate in the biotransformation process of the microorganism using a C1 gases such as carbon monoxide (CO) and methane (CH ₄₎ And a method for producing the nanofluid.

Cl gas, such as carbon monoxide (CO) and methane (CH ₄ ), has a limited development potential, but its technical limitations have so far limited its use in the field of simple energy recovery by combustion. In the case of carbon monoxide, the by-product gas, which is mainly generated in the combustion process in many processes, has a limited application in spite of the annual average of 6 tons of emission in Korea.

In particular, in the case of chemical processes, studies have been conducted mainly on methods for producing dimethyl ether and alkyl hydrocarbons from syngas in a well-known chemical process such as DME production technology and Fischer-Tropsh process, It corresponds to the indirect conversion technology using the high temperature and high pressure system, and there is a limitation that it is difficult to see a great advantage in terms of energy saving. In addition, due to technical limitations, catalysts are inactivated due to impurities contained in the byproduct gas, so that research on the combustion gas collection method for simple energy recovery is underway.

Methane gas is a major component of shale gas, which is distributed around the world and has estimated reserves of about 150 billion tons. It is mainly used for producing heat and power such as cogeneration, fuel cell and boiler heat source, Gas is injected directly into an automobile fuel or a city gas pipe network and is used as natural gas. SMR technology is widely known as a technology for using methane gas as a chemical raw material, and processes for converting methane into synthesis gas and using it as raw materials for various chemical products are being studied. However, these studies also have technical difficulties such as the need to adjust the mixing ratio of the synthesis gas to produce specific chemical raw materials. On the other hand, in the case of a biological conversion process using C1 gas, it is theoretically possible to directly convert C1 gas in a low-temperature and low-pressure process using a simple route using an organism, and to control the composition of the input gas and the influence of the impurities contained in the input gas And the selectivity of the product is high. However, since the reaction rate is very slow at the present stage, there is a problem that productivity is low to be utilized industrially.

Recently, researches on the development of bioenergy and chemical raw material technology using bacterial, archaeal and microalgae consuming C1 gas through specific metabolic process have been carried out. In particular, the microorganisms consuming Cl gas are thermophilic -bacteria , Halo-bacteria , Gram-positive bacteria , Methanogenic-bacteria , Methanotrophic bacteria have been found, and unlike microorganisms such as S. cerevisiae or E. coli , which multiply or ferment by using sugar as a substrate, carbon dioxide (CO ₂₎ carbon monoxide (CO) gas or by-product gases emitted during combustion, and methane (CH _4), propane (C ₃ H ₈₎ a bio-methanol industrially value by using such as (CH ₃ OH), bio It produces various raw materials such as hydrogen (H ₂ ), propanol (C ₃ H ₆ OH), acetic acid, succinic acid, maleic acid and formate. Therefore, research on microorganisms using C1 gas or by-product gas generated during combustion has been actively carried out with a focus on utilization in bio-engineering. However, The major problems are 1) low solubility in the liquid phase of C1 gas or byproduct gas in the reactor, 2) low mass transfer efficiency (K _L a), and 3) microbial growth rate and fermentation rate using C1 gas or byproduct gas It is slow, and this disadvantage is greatly highlighted.

Therefore, various high-concentration cell culturing methods such as cell recycling and membrane reactor have been proposed for improving the growth rate and productivity of microorganisms using C1 gas or by-product gas. However, in practical application through scale expansion, In the case of microorganisms using by-product gas, the mass transfer efficiency has the greatest effect on the growth concentration of microorganisms and the production rate of fermentation products, and therefore it is very difficult to introduce them into actual processes without improving the mass transfer efficiency.

In the process, the solubility of C1 gas is very low (solubility of carbon monoxide is 20 to 25 mg / L, methane solubility is 15 to 20 mg / L), and even if there are excellent bioconversion strains and catalysts, There is a fundamental limitation in improving productivity in the absence of process technologies that can be improved. In fact, according to a study by Professor Gaddy of the University of Arkansas in the United States, the carbon consumption rate of C. ljungdahlii , a microorganism using carbon monoxide, was 0.22 g _carbon / g _cell / (0.27 g _carbon / g _cell / hour) of the S. cerevisiae produced by the present invention .

Recently, various cell culture methods such as cell recirculation and membrane reactor have been proposed, but it is known that it is difficult to completely perform cell activation due to lack of mass transfer technology. In this context, in order to improve the mass transfer in the current gas-liquid system, the development of techniques for improving the mass transfer efficiency in the gas-liquid system is mainly performed, and in order to improve the mass transfer in the gas- Method as to: 1) generate the minute bubbles stably, and 2) increasing the rate of flow of gas and liquid, and, 3) sikimyeo the use or the high pressure of the particular solvent, increases the solubility of the gas, the gas according to 4) k _L a improved - Studies are under way based on strategies to increase the rate of mass transfer between liquids. In recent years, there have been many attempts to improve the process system and the mass transfer effect of the fermenter. However, since there is a disadvantage of high installation cost and reduction of energy saving efficiency in operation of the reactor, the mass transfer coefficient and solubility Nanofluids with nanoparticles with potential to improve are being studied.

A nanofluid is a fluid containing nanometer-sized particles, and usually refers to a stable dispersion of nanoparticles having a diameter of less than or equal to 100 nm in the fluid. Since it was first reported in 1995, research reports on nanofluids are on the increase. The application range is mainly theoretical analysis related to the improvement of heat transfer efficiency. Recently, the use of nanofluids has increased the mass transfer diffusion coefficient It is reported that the effect can also be expected. Specifically, studies using nanofluids mainly focus on mineral-based oxides, nitrides, and carbide ceramics, metals, metal oxides, and carbon nanotube-based nanoparticles to improve the mass transfer efficiency of oxygen, ammonia, and carbon dioxide And is applied to various fields such as boiling-phenomena, catalysts, surface-area, convection, mass-transfer, and bio-medical Research is underway. In the aspect of mass transfer, various studies on the gas absorption effect on gas-liquid equilibrium (Vapor-Liquid) have been reported. There are various hypotheses for why mass transfer is improved, 1) reduction of bubble size due to nanofluid; 2) reduction of thickness of gas-liquid interface by nanofluid; and 3) transport by C1 gas adsorption / desorption at the surface of nanoparticles forming nanofluid. And 4) mass transfer in the liquid phase by additional convective diffusion of nanoparticles.

As described above, although nanofluids can be utilized to effectively improve heat transfer and mass transfer in a wide variety of fields, there have been few studies on nanofluids applied in biological processes, It is because inorganic nanoparticles of 100 nm or less are known to flow into microbial cells and cause cytotoxicity. In terms of the mass transfer of the C1 gas bioconversion process, considering the influx of C1 gas into the cell, it is necessary to control the mass transfer barrier at the gas-liquid interface and the liquid phase as well as the material at the liquid- There is a transmission barrier. Since C1 gas such as carbon monoxide and methane flows into the cell by simple diffusion at the cell surface, it is considered that various types of cell membranes depending on the strain inhibit the inflow of C1 gas from the outside to the inside of the cell. Therefore, introduction of the nanofluid into the biotransformation process should solve the problem of cytotoxicity due to introduction of the nanofluid, and at the same time, improve the mass transfer efficiency by the conventional nanofluid, There is a further need for measures to eliminate the mass transfer barriers.

In this respect, it is desirable to improve the mass transfer efficiency that can be expected for conventional nanofluids without inducing cytotoxicity in the microorganism strain used in the C1 gas conversion process, and to overcome the mass transfer barrier of the liquid- There is a need to provide a new method for manufacturing nanofluids that can improve the mass transfer efficiency in the gas bioconversion process and to produce nanofluids using the nanofluids.

Accordingly, the present inventors prepared nanofluids containing organic nanoparticles prepared by using lipid compounds or nano-activated carbon, which are expected to interact with cell-friendly chitosan and cell membrane.

It is an object of the present invention to provide a nanofluid containing cell-friendly organic nanoparticles and to overcome the problem of cytotoxicity of a commonly known nanofluid and to improve the mass transfer efficiency of the gas in the liquid medium and the gas utilization of the microbial strain uptake efficiency of a nanofluid and a method of manufacturing the nanofluid.

The present invention

1) mixing chitosan with a lipid compound or nanocarrier,

Here, the lipid compound is at least one selected from the group consisting of oleic acid, lauric acid, oleamide, erucamide, monoglyceride, and sphingosine,

The diameter of the nano-activated carbon is 200 nm or less;

2) preparing nanofibers by electrospinning the solution prepared by mixing in the first step; And

3) crushing the prepared nanofibers in an aqueous solution having a pH of 9 or less;

The method comprising the steps of:

The present invention also provides a nanofluid prepared according to the method of manufacturing the nanofluid.

The present invention also provides a composition for promoting C1 gas conversion comprising the nanofluid.

The present invention also includes a nanofluid comprising at least one lipid compound selected from the group consisting of chitosan and oleic acid, lauric acid, oleamide, erucamide, monoglyceride, and sphingosine, or nanofluids having a diameter of 200 nm or less C1 < / RTI >

The present invention also

1) dividing and culturing microorganisms using C1 gas as a carbon source or an energy source; And

2) administering the nanofluid of claim 8 to the microorganism cultured in the first step; And

3) injecting C1 gas to perform the C1 gas conversion reaction;

The method comprising the steps of:

The nanofluid of the present invention makes it possible to efficiently use a gas having a low solubility in water in an aqueous solution, thereby increasing the C1 gas consumption rate of the microorganism using the C1 gas, increasing the productivity of the fermented product, and promoting the cell growth rate. The nanofluids containing the novel organic nanoparticles of the present invention also solve the cytotoxicity problem of nanoparticles of microbial strains in the bioconversion process.

1A shows a nanofiber prepared by using a mixed solution of chitosan and 0% oleamide. 1B shows nanofibers prepared using a mixed solution of chitosan and 10% oleamide. 1C shows nanofibers prepared using a mixed solution of chitosan and oleamide 50%. FIG. 1D shows a nanofiber prepared by using a mixed solution of chitosan and 100% oleamide. FIG. 1E shows a nanofiber prepared by using a mixed solution of chitosan and oleamide at 200%. FIG. 1F shows a nanofiber prepared by using a mixed solution of chitosan and oleamide 400%. Figure 1g shows the correlation between the amount of oleamide in the chitosan fiber and the average diameter of the nanofibers.
FIG. 2 is a graph showing the contact angle of nanofibers prepared using a mixed solution of 10%, 30%, 50%, 100%, and 200% by weight of oleamide relative to the weight of chitosan and chitosan.
FIG. 3A shows nanofluids prepared using a mixture solution of chitosan and oleamide having the same weight concentration, and nanoparticles forming nanofluids by scanning electron microscopy. FIG. 3b is a visual observation of the nanofluid produced.
FIG. 4A is a graph showing the relationship between the nanoparticle size and the dynamic light scattering when the nanofluid is prepared using the chitosan-oleamide mixed solution in which the concentration of the nanofluid solution is 0.025% Wherein the percentage of oleamide is the percentage of the oleamide weight relative to the weight of the chitosan. FIG. 4B is a graph showing the relationship between the size of nanoparticles and the size of the nanoparticles when a nanofluid is prepared using a chitosan-oleamide mixed solution in which the concentration of the nanofluid solution is 0.25% based on the total weight ratio, using dynamic light scattering Wherein the percentage of oleamide refers to the percentage of oleamide relative to the weight of chitosan.
5 is an analysis of the zeta potential of nanofluid prepared using distilled water.
FIG. 6A is an analysis of the sizes of nanoparticles forming nanofluids in various pH solutions. FIG. 6B is a graph showing the sizes of nanoparticles forming nanofluids in a pH range of 7.0 to 9.0. FIG.
FIG. 7A shows the average size change of nanoparticles when a nanofluid was prepared by applying surfactant Tween 20 to a chitosan / oleamide mixed solution for electrospinning. FIG. 7B shows the average size change of nanoparticles according to pH change when nanofluids were prepared by introducing surfactant Tween 20 into a chitosan / oleamide mixed solution for electrospinning.
8a to 8e illustrate changes in zeta potential in various pH solutions when the application method of the surfactant tween 20 is changed in the production of nanofluids. The results show that 100% by weight of oleamide nanofluids and 100% by weight of chitosan, When the surfactant Tween 20 is added to the oleamide nanofluid, the surfactant tween 20 is added to improve the stability of the nanofiber, and when the oleamide treated with the tween 20 is added to various pHs of the nanoparticles forming the nanofluid 8A to 8E are shown in FIG. Figure 8A shows the change in zeta potential at pH = 2. FIG. 8B shows the change in zeta potential at pH = 5. Figure 8c shows the change in zeta potential at pH = 7. Figure 8d shows the change in zeta potential at pH = 9. FIG. 8E shows the change in zeta potential at pH = 11.
FIG. 9 is a graph showing changes in particle size according to pH change of a nanofluid prepared by electrospinning and crushing in the same manner as in the chitosan / oleamide nanofluid production method using a mixed solution of chitosan and nano-activated carbon.
FIG. 10 shows the difference in cell growth rate when the nanofluid using the chitosan / oleamide / tween 20 mixed solution in the prepared nanofluid was applied to Thermococus onnurus NA1, which is a hydrogen producing strain using carbon monoxide. will be.
FIG. 11 shows the difference in cell growth rate when the nanofluid using the chitosan / nano-activated carbon mixed solution in the prepared nanofluid was applied to Thermococus onnurus NA1, which is a hydrogen producing strain using carbon monoxide.
FIG. 12 shows changes in carbon monoxide consumption when the nanofluid using the chitosan / oleamide / tween 20 mixed solution in the prepared nanofluid was applied to Thermococus onnurus NA1, which is a hydrogen producing strain using carbon monoxide .
FIG. 13 shows that when the nanofluid using the chitosan / oleamide / tween 20 mixed solution in the produced nanofluid was applied to Thermococus onnurus NA1, which is a hydrogen producing strain using carbon monoxide, the production amount of hydrogen as a fermentation product .
FIG. 14 shows changes in the amount of formic acid produced when the nanofluid using the chitosan / oleamide / tween 20 mixed solution in the prepared nanofluid was applied to acetobacterium woody, a strain producing formic acid using carbon monoxide.
FIG. 15 shows that, when the nanofluid using the chitosan / oleamide / tween 20 mixed solution in the prepared nanofluid was applied to Gram-negative microorganism DH-1, which grows using methane (CH ₄ ) And the growth rate.
FIG. 16 shows that when the nanofluid using the chitosan / oleamide / tween 20 mixed solution in the prepared nanofluid was applied to the methylhydrocinosporium type 5, which is a methanotrophic bacterium that proliferates using methane (CH ₄ ) Indicating the difference in cell growth rate.

The present invention provides a method for producing nanofluids comprising nanoparticles prepared by mixing nanoparticles comprising chitosan and a lipid compound or activated carbon.

The nanoparticles containing the chitosan and the lipid compound or nanocarbons of the present invention can improve the mass transfer efficiency of gas to microbial cells as a nanofluid composition in an aqueous solution and can be mass-produced in nanofluid form, Can be used to make.

The present invention relates to a method for preparing chitosan, comprising the steps of 1) mixing chitosan with a lipid compound or nano-

Here, the lipid compound is at least one selected from the group consisting of oleic acid, lauric acid, oleamide, erucamide, monoglyceride, and sphingosine,

The diameter of the nano-activated carbon is 200 nm or less;

2) preparing nanofibers by electrospinning the solution prepared by mixing in the first step; And

3) crushing the prepared nanofibers in an aqueous solution having a pH of 9 or less;

The method comprising the steps of:

The chitosan of the present invention is a naturally occurring highly safe cationic polysaccharide having the structure of (1- > 4) 2-amino-2-deoxy- beta -D-glucan. Chitosan is a polymer in which a free amine group is formed by deacetylation of chitin obtained from natural crustaceans and the like. The chitosan may have a molecular weight of 1 to 500 kDa and a free amine group of 50% or more, preferably a chitosan having a molecular weight of 150 to 400 kDa and a free amine group of 80% or more, but the present invention is not limited thereto. Soluble chitosan and insoluble chitosan and chitosan derivatives in which the molecules of chitosan are changed can be included in the category of chitosan of the present invention in view of solubilization of chitosan, and water-soluble chitosan can be preferably used. The water-soluble chitosan may be at least one selected from chitosan HCl, chitosan acetate, chitosan glutamate, and chitosan lactate. Water-soluble chitosan is soluble in water because amine groups in the molecular structure form salts with hydrochloric acid or acidic substances such as acetic acid, glutamic acid, and lactic acid. Water-soluble chitosan has advantages such as easy removal of foreign substances. In addition, chitosan having the same molecular structure may be included in all of them without limitation.

The lipid compounds of the present invention refer to lipids reported to have antimicrobial activity and lipid derivatives functionally similar thereto. Preferred are fatty acids having aliphatic residues between 6 and 18 carbon atoms, such as oleic acid and lauric acid, oleamide, erucamide and fatty alcohols, Refers to lipid compounds and derivatives thereof such as fatty acid derivatives or monoglycerides and sphingosine, more preferably oleamides and erucamides, even more preferably those having structural homology with oleamides.

The nano-activated charcoal of the present invention means activated carbon having a diameter of 200 nm or less, which is a material having a strong adsorption property and most constituent materials being carbon.

The method for producing a nanofluid of the present invention comprises the steps of 1) mixing a chitosan with a lipid compound or nanotubes.

In the mixing step, the organic solvent includes trifluoroacetic acid (HFA), hexafluoroisopropanol (HFIP), hexafluoropropanol (HFP), etc. The acidic solvent includes acetic acid, hydrochloric acid and the like. In the present invention, a mixed solvent of TFA and HFIP is preferable. The concentration of the lipid compound dissolved in the solvent may be 10% to 400% (w / v), preferably 100% (w / v) relative to chitosan. The mixing temperature is preferably 40 to 60 DEG C, more preferably about 50 DEG C. [ It is desirable to perform the sonication at the time of mixing, so that the viscosity of the solution can be reduced.

The nanofluid production method of the present invention includes the step of 2) preparing nanofibers by electrospinning a solution prepared by mixing in the first step.

Electrospinning is a technique of forming fibers by using electrical attraction and repulsion generated when a polymer solution or molten polymer is charged to a predetermined voltage. The electrospinning process is capable of producing fibers with various diameters of several nanometers to several thousands of nanometers, has a simple equipment structure, is applicable to a wide range of materials, and has an advantage in mass production. Specifically, in order to perform the electrospinning process, the chitosan and the lipid compound may be dissolved in an organic solvent alone or in a mixed solvent of an organic solvent and an acidic solvent.

The spinning process can be performed using an appropriate spinning device while applying at the appropriate voltage. The voltage applied during the spinning process may be set within the range of 10 to 40 kV, more preferably 20 to 30 kV, so that a stable spinning process can be performed. Further, the spraying rate is preferably 0.2 ml / hour to 0.9 ml / hour, more preferably 0.4 ml / hour to 0.8 ml / hour.

After the electrospinning, the method according to the present invention may further comprise drying the produced nanofibers.

The method for producing a nanofluid of the present invention comprises the step of 3) crushing the prepared nanofiber in an aqueous solution having a pH of 9 or less.

The nanoparticles produced by the crushing step are nanoparticles capable of forming a nanofluid by retaining a dispersed phase in an aqueous solution. The chopped nanoparticles comprise chitosan and a functional equivalent chitosan composition, a lipid compound and a functionally similar lipid derivative Nanoparticles produced by organic materials. The nanoparticles contained in the produced nanofluids preferably have an average diameter of 200 nm or less.

The prepared nanofibers are pulverized by using an ultrasonic pulverizer or a high-pressure pulverizer in order to suspend them in an aqueous solution. The aqueous solution may be distilled water or an aqueous solution having a pH of 9 or less. It may also be a buffer solution containing various electrolyte cell culture components suitable for the process. The pulverized aqueous solution can be used by diluting the nanofluid containing the dispersion of nanoparticles directly or in an appropriate concentration with a cell culture medium.

In some cases, the surfactant may be further included in the third step. The surfactant that may be included is the same as the list of surfactants that may be included in the second step.

The present invention provides a nanofluid prepared by the above-described method.

The present invention also provides a composition for accelerating the conversion of a C1 gas comprising a nanofluid produced by the above production method. The composition according to the present invention makes it possible to efficiently use a gas having a low solubility in water in an aqueous solution, thereby increasing the C1 gas consumption rate of the microorganism using the C1 gas, increasing the productivity of the fermented product, and promoting the cell growth rate. The nanofluids containing the novel organic nanoparticles of the present invention also solve the cytotoxicity problem of nanoparticles of microbial strains in the bioconversion process.

The C1 gas may be any one selected from the group consisting of carbon monoxide, carbon dioxide, and methane.

The composition of the present invention may be characterized as being active at room temperature and high temperature, preferably at 0 ° C to 100 ° C, more preferably at 20 ° C to 100 ° C, even more preferably at 20 ° C to 80 ° C .

The present invention also relates to a pharmaceutical composition comprising a nanofluid comprising a chitosan and at least one lipid compound selected from the group consisting of oleic acid, lauric acid, oleamide, erucamide, monoglyceride, A composition for promoting gas conversion is provided. The composition of the present invention can be utilized in a dispersed phase at room temperature and high temperature, and is preferably used at 0 ° C to 100 ° C, more preferably at 20 ° C to 100 ° C, even more preferably at 20 ° C to 80 ° C.

Further, the composition of the present invention can be utilized under various pH conditions, preferably pH 2 to pH 9, more preferably pH 2 to pH 7.

The composition of the present invention can be used without limitation to a microorganism using a by-product gas generated upon combustion. Specifically, the microorganisms using the C1 gas of the present invention as a carbon source or an energy source include microorganisms using Cl gas (carbon monoxide, carbon dioxide, methane), more preferably Gram-negative bacteria using Cl gas, Gram-positive bacteria, Archaea , Thermophilic- bacteria, Halobacteria , Methanogenic- bacteria, Methanotrophic- bacteria, Acetic acid-producing bacteria, Acetogenic- bacteria and Hydrogenic bacteria may be included, and more preferably, Methanotrophic bacteria, Acetogenic- bacteria and Hydrogenic bacteria may be included.

The methanotrophic bacteria of the present invention may be selected from the group consisting of Methylomonas sp. DH-1, Methylosinus sporium type 5, Methylosinus tricosporium OB3b Methylosinus tricosporium OB3b, Methylomicrobium alcaliphilum 20z, Methylococcus capsulatus, Methylosphaera hansonii, Methylomicrobium agile, , library bakteo micro emptying album as methyl (Methylomicrobium album), methyl hwiten (Methylobacter whittenburyi), bakteo base Proteus (Methylobacter luteus), bakteo binel randiyi (Methylobacter vinelandii) to bakteo Vorbis (Methylobacter bovis) methyl, methyl, Methylomicrobium pelagicum, Methylomicrobium sporium, Methylomonospores, nas Strain, Methylomonas rubra, Methylomonas aurantiaca, Methylomonas fodinarum, Methylomonas methanica, Methylokonucus thermolum, Methylococcus thermophilus, Methylococcus sporium, Methylococcus capsulatas, Methylomonas trisporium, Methylosinus trichosporium , Methylocystis parvus, Methylocella palustris, Methylocapsa acidiphila, Methylomonas buryatense, Methylocystis spolium, Methylcholinesterase , Methylocystis parvus, Methylocella palustris, Methylocapsa acidiphila, Methylomonas buryatense, (Methylocystis sporium), Sinners methyl tricot sports Solarium (Methylosinus trichosporium), Candida tooth meth furnace Pere de dense (Candidatus Methanop erededens, nitroreducens, Methylosarcina fibrate, Methylocaldum szegediense, Candidatus Methylomirabilis limnetica, Methylobalcomycin, But is not limited to, at least one selected from the group consisting of Methylovulus psychrotolerans .

Acetogenic bacteria of the present invention include Acetobacterium woodii, Clostridium autoethanogenum, Clostridium carboxidivorans P7, Clostridium carboxydivorans P7, (Clostridium ljungdahlii), no Pasteurella Thermo acetoxy urticae (Moorella thermoacetica), oil cake Te Solarium remote island (Eubacterium limosum), carboxy diborane lance (carboxidivorans), oxo bakteo pen nigiyi (Oxobacter pfennigii), pepto streptococcus a production tooth (Peptostreptococcus but are not limited to, productus , Acetobacterium woodii, Butyribacterium methylotrophicum, Methanosarcina acetivorans, Moorella thermoautotrophica, Desulfotomaculum kuznetsovii, Desulfotomaculum thermobenzikumum (Desulfoto maculum thermobenzoicum, Thermosyntrophicum, and Archaeoglobus fulgidus. However, the present invention is not limited thereto.

The Hydrogenic bacteria may be selected from the group consisting of Rubrivivax gelatinosus, Rhodopseudomonas palustris, Rhodospirillum rubrum, Citrobacter sp Y19, There may be mentioned, for example, Moorella AMP, Carboxydothermus hydrogenoformans, Carboxydibrachium pacificus, Carboxydocella sporoproducens, Carboxydosella thermoautos, The present invention relates to a medicinal composition containing at least one compound selected from the group consisting of Carboxydocella thermoautotrophica, Thermincola carboxydiphila, Thermolithobacter carboxydivorans, Thermosinus carboxydivorans , Desulfotomaculum carboxydivorans ), Thermococcus strain AM4 (Thermococcus strain AM4), Thermococcus onnulinus (Thermococcus onnurineus) , but the present invention is not limited thereto.

Microorganism using said C1 gas as a carbon source or energy source is most preferably carbon monoxide (CO) and methane (CH ₄₎ Thermo nose kusu Onnuri Taunus NA1 (Thermococcus onnurineus NA1) (KCTC10859 ), acetonitrile tumefaciens woodiyi (Acetobacterium use. Woodii ), Methylomonas sp. DH-1 and Methylosinus sporium Type-5 can be applied.

The present invention relates to: 1) a step of dividing and culturing microorganisms using C1 gas as a carbon source or an energy source; And

2) administering the nanofluid of claim 8 to the microorganism cultured in the first step; And

3) injecting C1 gas to perform the C1 gas conversion reaction; The method comprising the steps of:

When performing the C1 gas conversion method, C1 gas from bio-methanol (CH ₃ OH), bio-hydrogen (H _2), propanol (C ₃ H ₆ OH), acetic acid (Acetic acid), succinic acid (Succinic aicd), maleic Maleic acid, and formate. However, the present invention is not limited thereto, and any compound which is not included in the compound may be further obtained.

The C1 gas conversion method of the present invention includes 1) a step of dividing and culturing microorganisms using C1 gas as a carbon source or an energy source.

The culture of the microorganism can be cultured under any medium condition in which the normal stomach microorganism is viable.

The C1 gas conversion method of the present invention includes the step of 2) administering the nanofluid of claim 8 to the microorganism cultured in the first step.

In the step of administering the nanofluid to the cultured microorganism, it is preferable to administer the nanofluid to a final concentration of 0.0001 to 0.01% (w / v).

The C1 gas conversion method of the present invention includes 3) performing a C1 gas conversion reaction by injecting a C1 gas. The injection of the C1 gas may be the injection of the C1 gas itself, or a mixed gas of the C1 gas and the nitrogen gas.

The microorganism using the C1 gas of the present invention as a carbon source or an energy source is the same as the list of microorganisms defined as a microorganism using the C1 gas as defined above as a carbon source or an energy source.

Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples.

Example 1. Fabrication of nanofibers by electrospinning using chitosan and oleamide

The medium chain length (Sigma) was mixed with TFA / HFIP mixed solution at a ratio of 8: 2 by volume of TFA (Trifluoroacetic Acid) and HFIP (1,1,1,3,3,3-hexafluoro-2- Was added to a glass vial such that the weight ratio of echitosan was 6.5% of the weight of the mixed solution. The oleamide was then added to the glass vial at various ratios to the weight of the chitosan. In addition, the glass bottle containing the solution was placed in a water bath set at 50 DEG C and subjected to sonication for 30 to 90 minutes so as to reduce the viscosity of the solution in the glass bottle. The solution with reduced viscosity was stirred with a magnetic bar at 60 to 90 rpm for about 12 to 24 hours. The prepared solution was transferred to a 5 ml syringe having a diameter of 12.46 mm, and then a metal needle having a diameter of 0.5 mm was bonded and applied to the electrospinning. Thereafter, the distance between the metal needle and the collectors was fixed at 15.9 cm, and the electrospinning was conducted at a relative humidity of less than 30% and at a temperature of 20 to 35 ° C. The voltage at the time of electrospinning is set at 20 kV to 30 kV, the feed rate is controlled at 0.4 ml / hr to 0.8 ml / hr, and the uniformity in the collector plate for 5 to 12 hours depending on the amount of the prepared solution Of the nanofibers were recovered. The recovered nanofibers were dried for one day to two days or at a vacuum dryer at 65 ° C for one day in a humidity-free reagent control stand. The results of production of nanofibers analyzed by a scanning electron microscope (SEM) are shown in FIGS. 1A to 1G. FIG. 2 shows the degree of hydrophilicity of the nanofibers as the oleamide content of the chitosan nanofibers is increased.

Example 2. Preparation of nanofluids containing chitosan / oleamide nanoparticles

The nanofiber prepared in Example 1 was added in an amount of 0.01 g to 1 g to a 50 ml Falcon Tube containing 10 ml of the third distilled water according to the required nanofluid concentration, To 60%, operation time: 3 seconds, non-operation time: 3 seconds, and then crushed for 20 to 30 minutes. FIGS. 3A and 3B show the results of observation of nanoparticles contained in a nanofluid prepared by disrupting nanofibers containing 100% oleamide relative to chitosan by a scanning electron microscope, and the nanoparticles dispersed in nanofluids in an aqueous solution . 4A and 4B are graphs showing the relationship between the sizes of the nanoparticles containing the nanoparticles and the nanoparticles when the nanofluids were prepared in distilled water using 0.025% and 0.25% concentration of chitosan oleamide mixed solution, respectively, using dynamic light scattering The results of the analysis are shown. When the Zeta Potential of the nanoparticles was analyzed, it was confirmed that the dispersed phase was stable at a value of 40 mV or more (FIG. 5).

In order to analyze the stability of the nanofluid dispersion phase according to the pH change of the nanofluid, the particle size of the nanofluid prepared in distilled water was analyzed by dynamic light scattering method. As shown in FIGS. 6A and 6B, when the pH of the chitosan / oleamide nanoparticles was below 7, the size of the nanoparticles remained stable at a level below 100 nm. However, when the pH of the chitosan / oleamide nanoparticles was changed to a basic condition exceeding pH 7, coagulation rapidly occurred Respectively. However, when the nanofluid solution containing surfactant was added to the neutral and basic solution, the particle size of the dispersed phase gradually increased, but the stable dispersion state could be maintained at pH 9 or below. On the other hand, when chitosan / oleamide nanoparticles containing a surfactant were prepared by adding a certain concentration of a surfactant to a chitosan / oleamide mixed solution and electrospunning to obtain nanofibers and breaking the nanofibers, Was similarly maintained at a level of 100 nm or less. At the same time, even if the surfactant is not contained in the neutral and basic buffer solutions, it was confirmed that the size of the nanoparticles gradually increased with increasing pH, but the dispersed phase could be stably maintained and utilized (FIGS. 7A and 7B). In addition, when surfactant Tween 20 was added to 100% oleamide nanofluid versus chitosan and 100% oleamide nanofluid versus chitosan, and surfactant tween 20 was added to produce nanofibers, the stability of the nanofluid The change in zeta displacement in various pH solutions for the nanoparticles was analyzed and shown in Figs. 8A to 8E.

Example 3: Preparation of nanofluids using a mixture solution of chitosan and nano-activated carbon

Using the same method as the chitosan / oleamide-based nanofluid manufacturing method, it was investigated whether nanofluids could be prepared by stably dispersing nano-sized activated carbon, which is known to exhibit a variety of gases and strong adsorption ability, in an aqueous solution. As a result, nanotubes having a particle size of 200 nm or less, which are commercially available, were used to prepare a mixed solution of chitosan and activated carbon, and the nanofibers were prepared by electrospinning the nanotubes, and the nanoparticles were dispersed in a similar manner . As in the case of oleamide, the dispersion phase was stably maintained at a pH of 8 or less, and in the pH region larger than pH 8, the particle size of the dispersed phase (Fig. 9).

Example 4. Thermococus Onnurinus NA1 ( Thermococcus onnurineus  Comparison of CO consumption / hydrogen production / cell growth rate analysis using nanofluids for NA1

The nanofluid using the chitosan / oleamide / tween 20 mixed solution and the chitosan / nano-activated carbon mixed solution in the prepared nanofluid were applied to Thermococus onnurus NA1, which is a hydrogen producing strain using carbon monoxide , And how the cell growth rate / CO consumption / hydrogen production changes according to the applied nanofluid concentration as compared with that of the thermococcus onninus NA1 strain itself.

First, 10 ml of nanofluid was added to a 50 ml Falcon tube, and then sterilized for 30 minutes to 2 hours using ultraviolet (UV) light at a bio-safety test stand. Then, sterilized water 1L NaCl 35g, KCl 0.7g, CaCl 2 · 2H2O 0.4g, NH 4 Cl 0.3g, cysteine -HCl 0.5g, yeast extract _{10g, CuSO 4 · 5H 2 O} 0.04g, ZnSO 4 · 7H 2 per 0.4 g of CoCl ₂ .6H ₂ O, 0.8 g of MnCl ₂ .4H ₂ O, 0.4 g of Na ₂ MoO ₄ .2H ₂ O, 0.2 g of KBr, 0.2 g of KI, 0.4 g of H ₃ BO ₃ , 0.2 g of LiCl, 0.2 g of Al ₂ (SO ₄ ) 3, 0.04 g of NiCl ₂ .6H ₂ O, 0.02 g of VOSO ₄ .2H ₂ O, 0.02 g of H ₂ WO _4, 0.02 g of Na ₂ SeO ₄ , 6H ₂ O, 0.02 g of BaCl ₂ , 0.005 g of paranaminobenzoic acid, 0.002 g of Biotin, 0.005 g of calcium pantothenate, 0.0001 g of cyanocobalamin (B12), 0.002 g of folic acid, 0.005 g of nicotinic acid, 0.005 g of riboflavin, 0.005 g of thiamin-HCl and 0.005 g of lipoic acid (hereinafter referred to as NM1 medium). Subsequently, 36 ml of the NM1 medium prepared in 100 ml of glass serum bottle was dispensed, and 2 ml of Thermococcus onnurineus NA1 156T seed belonging to the genus Thermococcus was dispensed. The sterile nanofluid was then fed to a 100 ml glass serum bottle to a final concentration of 0 to 0.01% (w / v). Sterile water was then fed to a final volume of 40 ml of all samples. Next, using a rubber stopper and an aluminum seal, an inlet of a 100 ml glass serum bottle was closed, a 3 ml syringe needle was inserted into one side of each of 100 ml glass serum bottles containing nanofluid, and nitrogen (N ₂ ) Gas was supplied at a rate of 100 cc / min for 20 minutes to maintain the inside of the glass serum bottle under the anaerobic culture condition. Then, carbon monoxide (CO) gas was supplied at a rate of 100 cc / min for 30 minutes, followed by batch culture at 80 ° C for 6 hours. The growth rate of the cultured microorganisms was measured by using a spectrophotometer at 600 nm and the growth rate of microorganisms was compared. The amount of carbon monoxide (CO) gas consumption and hydrogen (H ₂ ) Was measured. The column used for gas chromatography was Agilent's Carboxen-1000 (mesh 60/80).

As shown in FIG. 10, when the nanofluids containing the nanoparticles prepared from the chitosan / oleamide / tween 20 mixed solution were applied, the cell growth rate tended to decrease slightly in the early stage and the nanoparticle concentration gradually increased And the growth rate of the strain was increased in proportion to the amount of the strain.

Also, as shown in FIG. 11, the cell growth rate of the Thermococus onnulinus NA1 strain was increased in the NM1 medium to which the nanofluids and the chitosan / nano-activated carbon mixed solution were applied using the chitosan / oleamide / tween 20 mixed solution I could confirm.

On the other hand, the amount of carbon monoxide consumption and the amount of hydrogen production of the Thermococcus onninus strain NA1 in the NM1 medium to which nanofluids containing nanoparticles prepared by mixing chitosan / oleamide / tween 20 were applied were also compared with the control group.

12 and 13, it was confirmed that the amount of hydrogen production also increased at the nanofluid application concentration where the CO consumption was increased.

It is presumed that this is due to the use of enzyme extract, which is a carbon source other than CO, due to the cell membrane affected by nanofluid.

Example 5. Acetobacterium woody applied with nanofluid ( A.woodii ) Formal Productivity of CO Using CO

It was confirmed that the productivity of formic acid was changed compared with that of the strain itself by applying to the acetobacterium woody strain producing formic acid using nanofluid and carbon monoxide using chitosan / oleamide / tween 20 mixed solution in the prepared nanofluid .

First, a microorganism culture medium prepared in a container (for example, a glass serum bottle) located inside the anaerobic chamber was prepared and dispensed. At this time, 0.2 g of KH ₂ PO ₄ , 0.25 g of NH ₄ Cl, 1.16 g of NaCl, 1.45 g of MgSO ₄ .7H ₂ O, 0.11 g of CaCl ₂ , 0.50 g of KCl, 2.0 mg of biotin, 0.04 mg of folic acid, 0.2 mg of pyridoxine hydrochloride, 0.1 mg of thiamine hydrochloride, 0.1 mg of riboflavin hydrochloride, 0.1 mg of nicotinic acid, 0.1 mg of DL-pantothenic acid, 0.002 mg of vitamin B12, 0.10 g of para-aminobenzoic acid 0.1 Nitric acid (N ₂ ) gas was added to the solution at about 100 ° C. for 1 hour. After the addition of 0.1 mg of L-histidine, 0.15 g of liposanic acid, 0.15 g of Na ₂ S.9H ₂ O, 0.3 g of cysteine hydrochloride, 1 ml of a trace element solution and 1 ml of selenite- Lt; RTI ID = 0.0 > cc / min < / RTI > for about 20 minutes to form an anaerobic condition. Subsequently, the medium prepared in the anaerobic chamber was dispensed into each 100 ml glass serum bottle in a volume of 40 ml, and then Gram-positive bacteria, Acetobacterium woody, were dispensed, And sealed with a rubber stopper and an aluminum seal. After incubation in a 37 ° C shaking incubator for about 16 to 19 hours, the absorbance (OD ₆₀₀ ) at ₆₀₀ nm was measured using a UV-spectrometer, and when it reached 1.5 or more, the concentration of acetobacterium woody The cells were transferred from the chamber to a 50 ml Falcon tube, sealed, and then the medium and microorganisms were separated using a centrifuge at 11.300 g and 4 ° C for 10 minutes. Then, the microorganism Acetobacterium woodyman was recovered And then washed twice with imidazole buffer (50 mM imidazole, 20 mM MgSO 4, 20 mM KCl, 4 mM DTE, pH 7.0) in the anaerobic chamber. Subsequently, the microorganisms recovered together with 10 ml of the imidazole buffer containing 20 mM NaCl and 30 μM ETH2120 in an anaerobic condition were dispensed into a 50 ml glass serum bottle at a concentration of 1 mg / ml, Was added in such a manner that final concentrations thereof were 0.045% and 0.1% (w / v), respectively. Thereafter, the container was sealed with a rubber stopper and an aluminum seal, and a 3 mL syringe needle was inserted into one side of each sealed 50 mL glass serum bottle to introduce nitrogen (N ₂ ) gas and carbon monoxide (CO) gas Was charged at a rate of 100 cc / min in a mixed gas at a ratio of 1: 1 for 10 minutes. Then, the syringe needle was carefully removed, and the reaction was continued for 24 hours in a 37 ° C shaking incubator while maintaining the inner anaerobic state of the glass serum bottle. High performance liquid-chromatography (HPLC) was then used to compare the amount of formic acid produced. The column used for the analysis was Hi-Plex H (7.7 * 300 mm 8 um, UV 210 nm) .

As can be seen from FIG. 14, the amount of formic acid produced when the nanofluid was used was significantly increased compared to the control.

Example 6 Pseudomonas genus as methyl (Methylomonas sp.) When nanofluids are applied to DH-1 cell Growth rate comparison

10 ml of each of the nanofluids prepared in Example 3 was added to a 50 ml nanofluid Falcon tube using a chitosan / oleamide / tween 20 mixed solution. Then, 10 ml of each of the nanofluids was irradiated with ultraviolet rays (UV) And sterilized for 30 minutes to 2 hours. Then, 1 g of MgSO ₄ .7H ₂ O, 1 g of KNO ₃ , 0.2 g of CaCl ₂ .2H ₂ O, 0.0038 g of iron chelate, 0.0006 g of Na ₂ MoO ₄ .2H ₂ O, 0.26 g of KH ₂ PO _{4 , Na 2 HPO 4 · 7H 2} O 0.62g, CuSO 4 · 5H 2 O 2.5㎎, 0.02㎎ biotin, folic acid 0.02㎎, thiamine hydrochloride 0.05㎎ 0.05㎎ potassium pantothenate, vitamin -B12 0.001㎎, 0.05㎎ riboflavin, nicotinamide _{0.05㎎, FeSO 4 · 7H 2 O} 0.5㎎, ZnSO4 · 7H 2 O 0.4㎎, MnCl 2 · 7H 2 O 0.02㎎, CoCl 2 · 6H 2 O 0.05㎎, NiCl 2 · 6H 2 O 0.01㎎, boric acid 0.015 Mg, and 0.25 mg of EDTA was prepared. 45 ml of the prepared NMS medium was dispensed in a 500 ml flask, and 4 ml of the methylmonomer DH-1 seed belonging to Gram-negative bacteria consuming methane was dispensed. Subsequently, 1 ml of sterile nanofluid was fed into a 500 ml flask such that the final concentration was 0.0001%, 0.001%, 0.01% (w / v) in the solution. In the control group, 1 ml of sterilized water was added to adjust the volume to 50 ml. Thereafter, the inlet of the 500 ml flask was closed using a rubber stopper and a flask cap. A 5 ml syringe needle was inserted into one side of each 500 ml flask containing the nanofluid, and a mixed gas of methane (CH ₄ ) gas and air gas (6: 4) was fed at a rate of 300 cc / min After incubation for 8 minutes and 30 seconds, the cells were incubated at 32 ° C for a certain period of time. Cell viability was measured by measuring the absorbance at 600 nm using a spectrophotometer.

As shown in FIG. 15, when the nanofluid was cultured in NMS medium at a concentration of 001% (w / v), it was confirmed that the cell growth was enhanced when DH-1 nanofluid, which was a control group, was used after culturing for 16 hours. In addition, since DH-1 does not provide a separate carbon source in addition to methane gas during culture, it is considered that the improved cell growth consumes more methane gas than the control group.

Example 7. Methylosynthosporium type -5 ( Methlylosinus sporium . Type-5) when the nanofluid is applied to the cell growth rate analysis

10 ml each of nanofluid using a chitosan / oleamide / tween 20 mixed solution was put into a 50 ml Falcon tube and sterilized for 30 minutes to 2 hours using a UV light at a biohazard laboratory. Then, 1 g of MgSO ₄ .7H ₂ O, 1 g of KNO ₃ , 0.2 g of CaCl ₂ .6H ₂ O, 0.0002 g of iron ammonium citrate, 0.0004 g of EDTA sodium salt, 0.26 g of KH ₂ PO ₄ , Na ₂ HPO _{4 · 7H 2 O 0.62g, FeSO 4} · 7H 2 O 0.1㎎, ZnSO4 · 7H 2 O 0.005㎎, MnCl 2 · 4H 2 O 0.0015㎎, CoCl 2 · 6H 2 O 0.01㎎, NiCl 2 · 6H 2 O 0.001㎎ , Boric acid (0.015 mg) and EDTA (0.25 mg) were mixed to prepare an ATCC medium (NMS 1306). A frequency divider for 45㎖ 500㎖ the ATCC production medium flasks then with methyl belonging to the methane bacteria magnetization (Methanotroph) Sinners sports Solarium And seeded with 4 ml of type-5 ( Methlylosinus sporium . Type-5) seeds. The sterilized nanofluid was then fed into the 500 ml flask at 1 ml to a final concentration of 0.001%, 0.0025%, 0.005%, 0.01% (w / v) in the solution. In the control group, 1 ml of sterilized water was added to adjust the volume to 50 ml. Thereafter, the inlet of the 500 ml flask was closed using a rubber stopper and a flask cap. A 5 ml syringe needle was inserted into one side of each 500 ml flask containing the nanofluid, and a mixed gas of methane (CH ₄ ) gas and air gas (6: 4) was fed at a rate of 300 cc / min After incubation for 8 minutes and 30 seconds, the cells were incubated at 32 ° C for a certain period of time, and then the absorbance was measured at 600 nm using a spectrophotometer to confirm the cell growth rate.

As shown in FIG. 16, the methylosynthosphosphereum type -5 of the control group showed a maximum growth of 34% in the concentration of the nanofluids. Similarly, in the case of methylosynthosphosphereum type-5, the cell growth was improved in comparison with the control group because methane gas was not provided in addition to the methane gas, which is considered to be due to consumption of methane gas.

Claims (17)

1) mixing chitosan with a lipid compound or nanocarrier,
Here, the lipid compound is at least one selected from the group consisting of oleic acid, lauric acid, oleamide, erucamide, monoglyceride, and sphingosine,
The diameter of the nano-activated carbon is 200 nm or less;
2) preparing nanofibers by electrospinning the solution prepared by mixing in the first step; And
3) crushing the prepared nanofibers in an aqueous solution having a pH of 9 or less;
≪ / RTI > The method according to claim 1, wherein the chitosan is at least one selected from the group consisting of water-soluble chitosan, insoluble chitosan and chitosan derivatives. 3. The method according to claim 2, wherein the water-soluble chitosan is at least one selected from the group consisting of chitosan HCL, chitosan acetate, chitosan glutamate and chitosan lactate. The method of claim 1, wherein the average diameter of the nanoparticles contained in the nanofluid is 200 nm or less. The method according to claim 1, wherein in the first step, chitosan and a lipid compound or nano-activated carbon are added to an acidic mixed solvent selected from the group consisting of trifluoroacetic acid, hexafluoroisopropanol, hexafluoropropanol, acetic acid, ≪ / RTI > The method of manufacturing a nanofluid according to claim 1, wherein a twin-system or a triton-type surfactant is further contained in the solution when the nanofiber is produced by electrospinning in the second step. 2. The method of claim 1, wherein in the second step, the electrospinning is performed at a flow rate of 0.4 ml / hr to 0.8 ml / hr and a voltage of 10 kV to 40 kV. A nanofluid produced by the process according to any one of claims 1 to 7. A composition for promoting C1 gas conversion comprising a nanofluid produced by the production method according to any one of claims 1 to 7. 10. The composition for promoting C1 gas conversion according to claim 9, wherein the C1 gas is at least one selected from the group consisting of carbon monoxide, carbon dioxide, and methane. Promoting C1 gas conversion including nanofluids comprising chitosan and at least one lipid compound selected from the group consisting of oleic acid, lauric acid, oleamide, erucamide, monoglyceride, and sphingosine; / RTI > 1) dividing and culturing microorganisms using C1 gas as a carbon source or an energy source; And
2) administering the nanofluid of claim 8 to the microorganism cultured in the first step; And
3) injecting C1 gas to perform the C1 gas conversion reaction; Gt; C1 < / RTI > 13. The method according to claim 12, wherein the C1 gas is at least one selected from the group consisting of carbon monoxide, carbon dioxide, and methane. 13. The method according to claim 12, wherein the microorganism using the C1 gas as a carbon source or an energy source is at least one selected from the group consisting of methane magnetism bacteria, acetic acid-producing bacteria, and hydrogen-producing bacteria. 15. The method according to claim 14, wherein the methanotrophic bacterium is selected from the group consisting of Methylomonas genus DH-1, Methylosynthospholium type-5, Methylorcinus tricosporium, Methylorubicium alkaline pyrazine, But are not limited to, methylcellulose, methylcellulose, hydroxypropylcellulose, hydroxypropylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, hydroxypropylmethylcellulose, But are not limited to, microorganism strains, microorganism strains, microorganism strains, methylromonas strains, methylromonas strains, methylromonas aurantiaca, methylromonas podinarum, methylromonas methanika, methylrococcus thermopillar, methylrocoquosporium, But are not limited to, cucurbituric acid, succinic acid, succinic acid, succinic acid, succinic acid, succinic acid, succinic acid, Pilar, methyl Monastir beak Oh Martens, Sinners in seutiseu Spokane Solarium, methyl City methyl tricot Spokane Solarium, Candida tooth meta Notre Pere de dense, nitro redu sense, to live with methyl Sinai fibrates, methyl local bush's me Dien's, Wherein the at least one gas selected from the group consisting of Candida albicans, Candida albicans, Candida albicans, and Candida albicans. 15. The method according to claim 14, wherein the acetic acid-producing bacterium is selected from the group consisting of Acetobacterium woody, Clostridium otetanogeninium, Clostridium carboxy diborance, Clostridium radix, Mullera thermoacetica, , Oxobacter filamentous fungi, peptostreptococcus prodactus, acetobacterium woody, butyryl bacterium methylotrophicum, methanosalcinase acetylborans, Mullera thermoautotrophica, Wherein the gas is at least one selected from the group consisting of desulfomaculum thermobenzoicum, thermosynthropicum, and aceoglobus perlidus. 15. The method according to claim 14, wherein the hydrogen-producing bacteria are selected from the group consisting of Lubribici boxel gelatinosus, Rhodopseudomonas palustris, Rhodospirillum lubrum, Citrobacterium strain Y19, Mullera AMP, Carboxydoserthus hydrogeloformans, Carboxydosaparasol prodose, carboxydosella thermoautotrophica, thermomicola carboxy dipillar, thermosyphobacter carboxy diborance, thermosynergy carboxy diborance, disulfomaculam carboxy diborance , Thermococus AM4, Thermococus onnurus, and the like.

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