KR20190044169A - Antiinflammatory composition comprising extracts of sonchus brachyotus - Google Patents

Abstract

The present invention relates to an anti-inflammatory composition comprising an extract of Sonchus brachyotus DC. as an active ingredient and, more specifically, to an anti-inflammatory composition comprising an extract of Sonchus brachyotus DC. as an active ingredient, which finds an anti-inflammatory effect of the extract of Sonchus brachyotus DC. and expands the related industry and contributes to the public health by applying the same to products such as soaps, cosmetics, or the like.

Description

TECHNICAL FIELD [0001] The present invention relates to an anti-inflammatory composition containing an extract of Sadecool as an active ingredient.

The present invention relates to an antiinflammatory composition containing an extract of Sadepur as an active ingredient, and more particularly, to an antiinflammatory effect of a sadepool extract and its application to products such as soap and cosmetics, And which can contribute to public health and health.

The human body suffers from many kinds of inflammatory diseases throughout its life. In particular, chronic irritable inflammation such as atopic dermatitis, allergic dermatitis, rheumatoid arthritis, bronchial asthma, and acne have not been established so far, so it is imperative to develop new drugs that can control and treat these diseases .

Inflammation is one of the defenses of biological tissue against external stimuli from cells and tissues. It causes cell reactions with nerves, blood vessels, tissues, blood and the like. As a result, if it is excessive, it may cause itching, fever, And the like. The inflammatory response begins with the recognition of the cell surface pattern that is specific to the pathogen as part of the innate immune response. Macrophages recognize cells with such specifically present cell surfaces as non-magnetic and attach to pathogens. The cause of inflammation is related to various biochemical phenomena. In particular, biosynthesis of nitric oxide synthase (NOS), which is an enzyme that produces nitric oxide (NO), and various prostaglandins (PGs) The mediating enzyme cyclooxygenase (COX) is an important mediator of the inflammatory response. Since NO and PGs are closely related to the inflammatory reaction, a substance capable of regulating the expression of the enzyme involved in the production and production of NO and PGs is attracting attention as a preventive and therapeutic agent for inflammatory diseases. Until now, the substances that have been used for antiinflammatory purposes are nasal steroids such as ibuprofen, indomethacin, etc. Steroids include prednisolone, dexamethasone, Antagonists, bradykinin antagonists, COX inhibitors and the like have been proposed. However, they have problems in terms of safety for skin and stability in formulation, and their use is limited.

Skin diseases called acne are skin diseases that occur in the hair follicles that show various skin lesions such as obstructive or open skin, papules, pustules, cysts, and nodules. The causes of acne include increased secretion of sebum, abnormal keratinization in follicles, and community formation of acne bacteria. These factors work together to cause acne lesions. That is, when the function of the sebaceous gland is enhanced by the secretion of the male hormone and the like, and the excess sebum generated thereby causes the follicular wall overgrowth and stagnates in the hair follicle, the follicle is formed, which is an early stage of the onset of acne. As the secretion of sebum increases and the hair follicle is excessively follicularized, sebaceous stasis in the hair follicles blocks the air circulation by blocking the hair follicle, which causes an environment in which the acne bacterium that resides in the hair follicle can grow well, do. In this case, acne bacterium secretes lipolytic enzymes and chemotactic factors to make free fatty acids and white blood cells around the hair follicle, and they stimulate and destroy the hair follicles, the contents of the hair follicles into the dermis into the early non-inflammatory acne inflammatory acne And it is exacerbated by purulent acne.

In general, the treatment of acne is accomplished by suppressing the cause of its occurrence. Examples of topical application agents used for treating acne by removing the above-mentioned acne bacteria include benzoyl peroxide, azelaic acid, retinoic acid, salicylic acid, Sulfur, topically applied antibiotics, and the like. However, these preparations do not exhibit sufficient antibacterial effect against acne bacterium only with a small amount, and there is a problem that side effects such as skin eruption or skin dryness may occur during excessive application.

Sadpool is a perennial herb that belongs to the genus Chrysanthemum (Compositae) and Sonchus which are mainly distributed in wet areas of reclaimed land, northeastern regions of Korea, Japan, China and Russia, etc. The present inventors have used this to confirm the anti- Or cosmetics, it has been found that the effect for improving skin troubles is excellent, leading to the present invention.

The object of the present invention is to solve the problems of the prior art as described above, and its object is to elucidate the anti-inflammatory effect of the sadepool extract and apply it to products such as soap and cosmetics And a sardine extract which can contribute to the public health as an active ingredient.

The technical problem of the present invention as described above is achieved by the following means.

(1) An antiinflammatory composition comprising Sadupool extract as an active ingredient.

(2) The composition for anti-inflammation according to (1) above, wherein the extract is an ethanol extract.

(3) The composition for anti-inflammation according to (2), wherein the extract is a 70% ethanol extract.

(4) An antiinflammatory composition, wherein the composition of (1) is a soap.

(5) The composition for anti-inflammation according to (1), wherein the composition is cosmetics.

As described above, according to the present invention, by manufacturing soap or cosmetics using the sadepool extract having an anti-inflammatory effect, it can contribute to expansion of related industry and the public health.

Fig. 1 shows the results of measurement of NO inhibitory activity using sadupool extract.
FIG. 2 shows the results of measurement of PGE ₂ inhibitory activity using Sadupool extract.

Hereinafter, the contents of the present invention will be described in more detail.

The composition for anti-inflammation according to the present invention contains Sadupool extract as an active ingredient.

In the present invention, the sadecool extract includes a hot-water extract, an ethanol extract, or an extract using a mixture of water and ethanol, or a supercritical extract.

In the case of the hot-water extract, it is preferable to add 1 to 10 times of water and extract at 1 to 2 atm and 80 to 120 캜 for 1 to 12 hours. The ethanol extract may be an extraction solvent composed of 50 to 100% ethanol, and the supercritical extract may be performed under the pressure of 250 to 350 bar using ethanol as an extraction solvent. In the present invention, particularly preferably, the above extract is a 70% ethanol extract.

The composition of the present invention can be formulated into a soap composition, and in the present invention, the soap composition is used to include both solid soap and liquid soap.

In the present invention, the raw material of the soap can be obtained by the following process.

The above-obtained sadpool extract obtained according to the present invention is mixed with a conventionally used soap base, flavor, moisturizing agent and the like, and the mixture is mixed by a conventional homemade method (stirring with a wooden spatula). At this time, the content of the sadecool extract is not particularly limited, but may be about 0.1 to 10% by weight.

The soap base was prepared by heating and dissolving the fatty acid at 65 to 68 ° C and then adding an alkali salt at 82 to 87 ° C to saponify the reaction mixture. The upper layer was collected, washed several times with brine and dried in vacuo to a moisture content of 15% Of the total amount of water.

At this time, as the soap base, a fatty acid soap base, a synthetic surfactant soap base, or a mixture of the fatty acid soap base and a synthetic surfactant soap base may be used.

The fatty acid-based soap base is easily obtained by saponifying the vegetable oil selected from palm oil, palm oil, soybean oil, castor oil, olive oil, palm kernel oil, and animal fats selected from tallow, lard and fish oil, have. As the synthetic surfactant soap base, conventional ones selected from sodium lauryl sulfonate, sodium salt of alkylbenzene sulfonate, sodium cocoyl isethionate, and alkali metal salts of acyl glutamate can be used. The fatty acid-based soap base and the synthetic surfactant-based soap base may be mixed with each other.

It is needless to say that the soap composition according to the present invention may contain at least one selected from fragrance, dye, fungicide, anti-inflammatory agent, antioxidant and preservative in addition to the above-mentioned sadupool extract.

The soap composition according to the present invention can be easily produced by compression molding by a conventional method using the soap composition having the above composition. In particular, in the case of the soap prepared according to the present invention, skin protection such as whitening, anti-aging, moisturizing, Effect can be obtained.

In addition, the sadepole extract obtained according to the present invention can be used variously in a cosmetic composition or a pharmaceutical composition for prevention and treatment of various inflammatory skin diseases.

The components included in the composition of the present invention include conventional adjuvants and carriers such as stabilizers, solubilizers, vitamins, pigments, and perfumes, including components commonly used in compositions other than the above-mentioned sadecool extract as an active ingredient .

The composition of the present invention may be prepared in any form conventionally produced in the art and may be in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, , An emulsion foundation, a wax foundation, and a spray, but is not limited thereto. More particularly, when the composition of the present invention is used as a cosmetic composition, it is formulated into a form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder .

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, chlorofluorohydrocarbons, propane / Or propellants such as dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butylene glycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate, alkyl Aliphatic alcohols, fatty acid glycerides, fatty acid diethanol amides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these embodiments are for the purpose of understanding the present invention and should not be construed as limiting the scope of the present invention.

[Example 1]

Sadpool was collected from Seokmodo, Ganghwa-gun, Korea, washed with water, and stored at -80 ℃ for 1 day. The frozen samples were dried for 4 days using a freeze dryer. The dried samples were pulverized using a pulverizer to prepare powder form. 70% ethanol was added to the dry powdery form and extracted eight times with an ultrasonic extractor at room temperature for 1 hour. Each extract was filtered using a filter paper, and the filtered extract was concentrated using a vacuum concentrator. The concentrated samples were freeze-dried at -80 ° C for 1 day and dried for 4 days using a freeze dryer for complete drying. The dried extract was stored in a powder form and stored at -80 ° C until used in the experiment.

[Example 2] Production of soap

3% by weight of the sadecool extract obtained in the procedure of Example 1, 87% by weight of soap base (moisture content 15%) added with caustic soda to woji and palm oil, 0.5% by weight of fragrance, 0.5% by weight of moisturizer, 0.3% 0.1% by weight of glycerin, 0.5% by weight of coconut fatty acid, 0.1% by weight of surfactant, and the remaining amount were mixed with a substance usually contained in soap, finely divided by a pulverizer and extruded using a soap maker to prepare soap. Comparative Example 1 produced the soap by the same procedure except that the sedu grass extract was not added in Example 2 and the soap base was constituted to 90 wt%.

[Example 3] Production of soap

5% by weight of the sadecool extract obtained by the procedure of Example 1, 85% by weight of soap base (moisture content 15%) added with caustic soda to woji and palm oil, 0.5% by weight of fragrance, 0.5% by weight of moisturizer, 0.3% 0.1% by weight of glycerin, 0.5% by weight of coconut fatty acid, 0.1% by weight of surfactant, and the remaining amount were mixed with a substance usually contained in soap, finely divided by a pulverizer and extruded using a soap maker to prepare soap.

[Example 4] Production of soap

10% by weight of the sadecool extract obtained by the procedure of Example 1, 80% by weight of soap base (moisture content 15%) added with caustic soda to woji and palm oil, 0.5% by weight of fragrance, 0.5% by weight of moisturizer, 0.3% 0.1% by weight of glycerin, 0.5% by weight of coconut fatty acid, 0.1% by weight of surfactant, and the remaining amount were mixed with a substance usually contained in soap, finely divided by a pulverizer and extruded using a soap maker to prepare soap.

[Example 5] Preparation of cream

The cream of Example 5 was prepared by the ingredients and contents shown in Table 1 below. Comparative Example 2 was the same as Example 5, except that the sadepole extract was not added.

Composition (% by weight) content Sadecool extract (Example 2) 10 Stearic acid 15 Cetanol 1.0 Potassium hydroxide 0.7 glycerin 5.0 Propylene glycol 3 antiseptic 1.0 incense 1.0 Purified water Balance

[Experimental Example 1] Measurement of NO inhibitory activity

RAW 264.7 cells were cultured in a 5% CO ₂ incubator by adding RPMI 1640 medium containing 10% FBS to a 24-well plate to measure NO inhibitory activity. The cells were subjected to artificial LPS treatment to induce NO concentration excessively, and then treated with Sadepul 70% ethanol extract at a concentration of 100, 200, 300 ug / mL, and the concentration of NO released from the cells was measured using Griess reagent Respectively. After 24 hours of incubation with the extract and LPS-treated cells, 100 μL of the culture medium was added to the 96-well plate, and 100 μL of the Giress reagent was added to the culture medium for 10 minutes in the dark And the NO concentration was measured at a wavelength of 540 nm.

The inhibitory effect of the 70% ethanol extract of Sadepul on the NO production in the concentration range not showing cytotoxicity was 88.65%, 68.37%, and 22.90% at the concentration of 100, 200 and 300 ug / ml, (Fig. 1).

[Experimental Example 2] Measurement of PGE ₂ inhibitory activity

To determine the PGE ₂ inhibitory activity of RAW 264.7 cell lines, the number of macrophage cells was measured, and the cells were cultured in a 5% CO ₂ incubator by adding RPMI 1640 medium containing 10% FBS to a 24-well plate. The cells were treated with LPS to induce excessive concentration of PGE ₂ , and then treated with Sadepul 70% ethanol extract at 100, 200, and 300 ug / mL to determine the concentration of PGE ₂ secreted from the cells. Linked Immunosorbant assay (ELISA) kit.

As a result of the inhibition of PGE ₂ production by the 70% ethanol extract of Sadepul without cytotoxicity as described above, the inhibitory effect of PGE _{2 on the} concentration of 100 μM, 200 μM and 300 μg / mL was found to be 51.84%, 40.04% and 12.11% (Fig. 2). ≪ tb >< TABLE >

[Experimental Example 3] Measurement of skin irritation mitigation effect

In general, how the compounds according to the present invention alleviate skin irritation caused by lactic acid, which is known to stimulate the skin, is examined. The cream of Example 5 and Comparative Example 2 was rubbed by a spatula each time by 20ul each on a circular portion of about 1.2 cm in diameter on the skin of 15 women.

At this time, the first two times were applied to the hill top chamber without the filter paper in the inside after the application of the composition, and the closure conditions were established by fixing with an adhesive. In the chamber reaction by the hilltop chamber, when the composition was applied three to eight times, the filter paper was placed immediately after application and fixed with an adhesive. Spreaded once every 23 hours, and inspected 1 hour after removal of the patches according to the CTFA guidelines. On the 4th day after the end of the patch, an additional test was performed to see the delayed type response and the result was reflected in the results. Data were analyzed by post-test (p <0.05) using ANOVA after confirming equal distribution of Levin test using the last test result. The results were classified as grade 0 (no visible response), grade 1 (+, weak erythema), grade 2 (++, strong erythema), grade 3 (+++, strong erythema and edema), grade 4 ++++, strong erythema, edema, blister), and the results are shown in Table 2 below. The results shown in Table 2 are the sum of the response indices of the subjects who responded to the stimulus by the number of times.

Number of Patches Example 5 Comparative Example 2 One 0 0 2 0 4 3 0 3 4 One 4 5 0 4 6 0 5 7 0 4 8 One 4 Four days after the end One 6 Sum 3 34

As shown in Table 2, the cream of Comparative Example 2 showed a very high skin irritation response by lactic acid, whereas the composition of Example 5 containing the sadepole extract of the present invention showed skin irritation Was relatively low.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It can be understood that

Claims (5)

A composition for antiinflammation comprising an extract of Sadecool as an active ingredient. The composition according to claim 1, wherein the extract is an ethanol extract. 3. The composition according to claim 2, wherein the extract is a 70% ethanol extract. The composition for anti-inflammation according to claim 1, wherein the composition is a soap. The composition for anti-inflammation according to claim 1, wherein the composition is cosmetic.

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