KR20190023324A - Imaging system for Obtaining multi-mode images - Google Patents

Abstract

The present invention relates to an image acquisition system capable of acquiring a bright field image, an optical differential phase difference image, and a quantitative optical phase image for a sample with single imaging. The image acquisition system includes a light source unit and an imaging unit for acquiring an image of the sample. The light source unit has a plurality of color patterns and simultaneously outputs a plurality of beams having different colors to the sample through the color patterns. The imaging unit receives the plurality of beams transmitted through the sample or reflected by the surface of the sample to generate each of a plurality of phase information for the sample and calculates phase information to obtain different images with respect to the sample. The image acquisition system includes a light source unit, a filter unit, and an imaging unit. The light source unit outputs light with respect to the sample. The filter unit has a pattern such that a plurality of different colors are given to the light output from the light source unit. The imaging unit receives the plurality of beams transmitted through the sample or reflected by the surface of the sample to generate each of a plurality of phase information for the sample and calculates phase information to obtain different images with respect to the sample.

Description

[0001] The present invention relates to an image acquisition system capable of acquiring a multi-mode image,

The present invention relates to an image acquisition system capable of acquiring both a bright field image, an optical differential phase difference image, and a quantitative optical phase image for a sample with only one photographing operation.

In general, optical microscopes are mainly used for medical and biological research purposes. Such an optical microscope is configured in such a manner that a light passing through the sample is irradiated with light, a real image magnified by the objective lens is formed by the sample, and the real magnified image is observed through the eyepiece lens.

However, since a general optical microscope is observed through a difference in contrast or color of a sample, there is a problem that a colorless transparent sample such as a living cell is not observed properly.

Accordingly, a phase contrast microscope has been developed in which a sample is observed in such a manner that the phase difference caused by the interference between the diffracted light and the non-diffracted light is expressed by the difference in contrast.

Such a phase contrast microscope is capable of observing a sample such as a living cell, which can not be observed in a conventional optical microscope, by converting the phase information changed by the refractive index difference into a light intensity distribution, but provides qualitative phase information , There was a limit to the precise analysis of biological specimens.

Thus, a microscope device has been developed that can provide quantitative optical phase information for a biological sample.

In order to obtain such optical phase information, two or more photographing operations are required. Specifically, in order to secure a quantitative optical phase image, a light source of a different pattern is irradiated, and the light source pattern is changed and photographed repeatedly to photograph two or more times, and one optical phase image is obtained each time the photographed image is photographed.

However, there is a problem that motion information loss due to a time difference exists in securing an image for a sample through two or more photographings. In addition, there is a problem that a photographing speed is limited to half or less, and a communication system for synchronization of a light source and photographing is required in response to photographing.

An object of the present invention is to provide a method and an apparatus for generating phase information of a sample using a plurality of lights having different colors and arithmetically processing the same to acquire a bright field image, an optical differential phase difference image, and a quantitative optical phase image, respectively, And an image acquiring system in which the image accuracy of a sample is improved while being inexpensive.

According to an aspect of the present invention, there is provided an image acquisition system including a light source unit and a photographing unit for acquiring images of a sample. The light source unit has a plurality of color patterns, and simultaneously outputs a plurality of lights having different colors to the sample through the color pattern. The photographing unit receives a plurality of lights transmitted through the sample to generate a plurality of phase information for the sample, and calculates a plurality of different images for the sample by calculating the phase information.

Further, the image acquisition system includes a light source section, a filter section, and a photographing section. The light source unit outputs light to the sample. The filter portion is formed with a pattern such that a plurality of different colors are given to light output from the light source portion. The photographing unit receives a plurality of lights transmitted through the sample to generate a plurality of phase information for the sample, and calculates a plurality of different images for the sample by calculating the phase information.

Further, the image acquisition system includes a light source unit and a photographing unit. The light source unit has a plurality of color patterns, and simultaneously outputs a plurality of lights having different colors to the sample through the color pattern. The photographing unit receives a plurality of lights reflected on the surface of the sample to generate a plurality of phase information for the sample, and calculates a plurality of different images for the sample by calculating the phase information.

Further, the image acquisition system includes a light source section, a filter section, and a photographing section. The light source unit outputs light to the sample. The filter portion is formed with a pattern such that a plurality of different colors are given to light output from the light source portion. The photographing unit receives the light reflected on the surface of the sample, generates a plurality of phase information for the sample, and computes phase information to acquire a plurality of different images for the sample.

According to the present invention, it is possible to obtain a bright field image, an optical differential phase difference image, and a quantitative optical phase image for a sample with only one photographing operation, so that the sample can be inspected more precisely and accurately.

In addition, since the bright field image, the optical differential phase difference image, and the quantitative optical phase image can be obtained by only one photographing, the photographing speed is increased and the movement information of the sample due to the time difference during photographing is prevented from being lost .

In addition, since red light, green light, and blue light are simultaneously output through only a low-cost LED array or filter, the initial installation cost can be reduced.

In addition, a bright field image, an optical differential phase difference image, and a quantitative optical phase image for the sample can be obtained by calculating the phase values of the lights obtained through the photographing unit. Therefore, since an expensive additional device such as a prism or an interference optical system is not required as in the prior art, the initial installation cost is reduced and the device can be easily implemented.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 schematically shows the configuration of an image acquisition system according to an embodiment of the present invention; FIG.
Fig. 2 is a drawing showing the light source portion in Fig. 1; Fig.
FIG. 3 is a view showing the bright field image, the optical differential phase difference image, and the quantitative optical phase image obtained through the photographing unit in FIG. 1; FIG.
FIG. 4 schematically shows a configuration of an image acquisition system according to another embodiment of the present invention; FIG.
Fig. 5 is a drawing showing the filter unit in Fig. 4; Fig.
Fig. 6 is a view showing a comparative example of an area of a region through which light defined by a Fourier plane can be transmitted and an area of light generated by a light source and transmitted to a Fourier plane, in Fig.
7 is a view schematically showing a configuration of an image acquisition system according to another embodiment of the present invention.
Fig. 8 is a view showing a comparative example of an area of light generated in the light source unit to be transmitted to the Fourier plane and an area of a region through which light defined in the Fourier plane can be transmitted, in Fig.
9 to 11 are views schematically showing a configuration of an image acquisition system according to another embodiment of the present invention.

Hereinafter, an image acquisition system according to a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings. Here, the same reference numerals are used for the same components, and repeated descriptions and known functions and configurations that may obscure the gist of the present invention will not be described in detail. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art. Accordingly, the shapes and sizes of the elements in the drawings and the like can be exaggerated for clarity.

FIG. 1 is a view schematically showing a configuration of an image acquisition system according to an embodiment of the present invention, and FIG. 2 is a drawing showing an excerpt of a light source portion in FIG. FIG. 3 is a diagram showing the bright field image, the optical differential phase difference image, and the quantitative optical phase image obtained through the photographing unit in FIG.

1 to 3, the image acquisition system 100 is for acquiring an image of a sample S, and includes a light source unit 110 and a photographing unit 120. Here, the sample (S) may be a biological tissue such as a transparent cell, but is not limited thereto. The image acquisition system 100 according to the present embodiment can be applied to a transmission type optical microscope.

The light source unit 110 has a plurality of color patterns 110a and simultaneously outputs a plurality of lights having different colors to the sample S through the color pattern 110a. Specifically, the light source unit 110 may be configured to output red light, green light, and blue light, respectively, and each color pattern 110a may be uniformly divided .

For example, when the light source unit 110 is circular and the color pattern 110a is divided into red (R), green (G) and blue (B) regions, . That is, the color pattern 110a may be formed so as to have an angle of 120 degrees from the center of the circle. In other words, a plurality of lights output from the light source 110 at different positions are formed to have different phases from each other. That is, different light phases are generated at different positions of the light source unit 110, and light generated at different positions of the light source unit 110 is classified as a color.

The light source unit 110 may include an LED array and a control unit.

The LED array outputs a plurality of lights having different colors. To this end, the LED array may include a plurality of light emitting diode arrays each of which is configured to emit beams of various colors. The light emitting diode array may be formed to output a plurality of lights having different colors through a known color pattern process.

The control unit is electrically connected to the LED array to change the brightness and color of the light output from the LED array.

The photographing unit 120 receives a plurality of lights transmitted through the sample S to generate a plurality of phase information for the sample S and calculates a plurality of phase information for the sample S, Obtain the image. Here, the different images denote the bright field image 10, the optical differential phase difference image 20, and the quantitative optical phase image 30, respectively.

The photographing unit 120 may include a color camera 121 and an operation unit 122.

The color camera 121 receives and photographs a plurality of light beams transmitted through the sample S and acquires images corresponding to phase information of the lights transmitted through the sample S, respectively. More specifically, the color camera 121 may be a CCD camera or a CMOS camera. The color camera 121 may include three image sensors for detecting red light, green light, and blue light, respectively . Accordingly, the color camera 121 can receive each of the plurality of lights through the image sensor and acquire respective color images.

Since the plurality of lights are received through the color camera 121 and can be separated for each color, the color information of the light sources can be separated at a low cost to acquire images.

The arithmetic operation unit 122 computes the phase information corresponding to each of the plurality of lights through the image. A process of calculating the phase information for the sample S through the calculation unit 122 will be described below.

First, the light source unit 110 outputs red light, green light, and blue light. Then, the color camera 121 receives the red light, the green light, and the blue light, and separately captures the color by the color.

At this time, since lights having different colors are outputted at the same time, there is a possibility that the frequency of the light arranged in the periphery may be invaded when the image is taken through the color camera 121. For example, when photographing red light through the color camera 121, part of green light or blue light is photographed together.

Therefore, it is desirable to correct the error that occurs when the calculation is performed through the calculation unit 122. For this purpose, it is preferable to apply a color leakage compensation method for correcting a phase error due to color leakage. The color leakage compensation method can be expressed by the following matrix.

은 컬러 카메라(121)의 각 이미지 센서에 의해 검출된 광의 정규화 된 색상 별 세기를 의미하며, l 은 색상 별 이미지 센서, k 는 입사되는 광의 색상을 의미한다. Here, I ^IMG is the intensity of the light incident on the image sensor of the color camera 121, and I ^CCD is the size of the signal of each color of the light detected by the image sensor of the color camera 121. And,

L denotes a color image sensor, and k denotes the color of the incident light. The color sensor 121 detects the color of the image sensor.

In this way, by applying the color leakage compensation method at the time of shooting the color camera 121, it is possible to prevent an error from being generated in the calculation through the following equations.

On the other hand, since the red image, the green image, and the blue image acquired through the color camera 121 each include different phase information with respect to the sample S, if the three images are used, The optical differential phase difference image 20, and the quantitative optical phase image 30 can be acquired.

First, a phase value I _R for an image of red light captured through the color camera 121, a phase value I _G for an image of green light, and a phase value I _B for an image of blue light are calculated , It is possible to acquire the bright field image (I _BF , 10). The equation is expressed as follows.

Next, the equation for obtaining the optical differential phase difference image 20 is as follows.

Here, l, m and n denote the colors of the respective light sources. For example, 1 means red light, m means blue light, n means green light, and u means space frequency coordinates. At this time, the relationship l? M, n is satisfied. That is, it is possible to obtain an optical differential phase difference image in three directions through three combinations of l, m and n.

Next, the optical differential phase difference image 20 can be reconstructed into a quantitative optical phase image 30 through a Phase Gradient Transfer Function or a Weak Object Transfer Function.

A method for acquiring a quantitative optical phase image 30 using a phase gradient transfer function is as follows.

The three optical differential phase difference images photographed through the color camera 121 can be converted into optical differential phase difference images in the x-axis direction and the y-axis direction through the following equations.

The converted optical phase difference image in the x-axis direction and the y-axis direction can be calculated as the optical phase slope image through the following phase gradient transfer function.

The calculated optical phase slope image in the x-axis direction and the y-axis direction is calculated as a quantitative optical phase image through Fourier phase integration, and the quantitative optical phase image 30 can be acquired by this calculation .

A method for acquiring a quantitative optical phase image 30 using a weak object transfer function is as follows.

When the absorption of light due to the sample S, the phase change, and the scattering are small, the transmission function due to the sample S can be expressed as follows.

와, 위상변화

를 포함하는 아래의 식으로 표현될 수 있다. At this time, the color-by-color image of each light source is absorbed by the light source caused by the sample (S)

And phase change

The following equation can be expressed.

,

는 광원의 흡수, 위상 변화의 묽은 물체 전달함수(Weak Object Transfer Function)이다. here,

,

Is a weak object transfer function of the absorption and phase change of the light source.

When the optical differential phase difference image is calculated through the above equation, it is possible to approximate the optical differential phase difference image having only the phase difference component, so that the quantitative optical phase image 30 can be obtained.

Meanwhile, the image acquisition system 100 may further include a guide unit 130 for guiding a plurality of lights output from the light source unit 110. The guide unit 130 includes a guide mirror 131 for guiding the paths of a plurality of lights output from the light source unit 110 and a guide mirror 131 for guiding the plurality of lights reflected by the guide mirror 131 to the image pickup unit 120 And a tube lens 132.

An objective lens 140 may further be provided between the sample S and the photographing unit 120. At this time, the sample S may be disposed on a support capable of transmitting light, and the objective lens may be disposed below the sample S.

4 is a view schematically showing the configuration of an image acquisition system according to another embodiment of the present invention. 4 is an enlarged view of the filter unit shown in Fig. 4, Fig. 6 is an enlarged view of Fig. 4 showing the area of light generated in the light source unit and transmitted to the Fourier plane, Fig. 2 is a view showing a comparative example of the area of the region where the semiconductor device can be formed. In the present embodiment, differences from the above-described embodiment will be mainly described.

4 to 6, the image acquisition system 200 includes a light source unit 210, a filter unit 220, and a photographing unit 230. Here, the image acquisition system 200 according to the present embodiment can be applied to a transmission type optical microscope.

The light source unit 210 outputs light to the sample S. Here, the light source unit 210 may be an LED array that outputs light of a single color, more preferably white light.

The filter unit 220 may be patterned such that a plurality of different colors are imparted to the light output from the light source unit 210. Specifically, the filter unit 220 is disposed at a lower portion of the light source unit 210 and includes a color filter 230 for extracting three colors of red (R), green (G), and blue (B) And a color filter.

When the filter unit 220 is disposed on the light source unit 210 (or when the light source unit is composed of color LEDs), the area L of the light source unit 210, the area of light generated in the light source unit and transmitted to the Fourier plane And the area L of the light source unit 210 is equal to the area of the area through which the light defined by the Fourier plane F can be transmitted . This is because when the direction of the light emitted from the light source unit 210 (the white light passing through the filter unit) is reflected by the sample due to the difference in refractive index between the sample and the periphery of the sample, that is, when the position of the Fourier plane F is changed, The area of the light source unit 210 (L, the area of light generated in the light source unit and transmitted to the Fourier plane) and the area of the light that is defined by the Fourier plane F can be transmitted, L) is larger than the area of the area of the light-transmitting area defined by the Fourier plane (F), the image sensor can recognize that a difference in intensity of red, green, and blue light has occurred.

In other words, if the area L of the light source 210 is smaller than the area of the light-transmitting area defined by the Fourier plane F, information about the direction of the light changed by the sample S can be lost It is because.

The filter unit 220 has a circular shape and is divided into three equal widths. The light passing through the divided filter unit 220 can be output as red light, green light, and blue light. In other words, light passing through the filter unit 220 may be outputted at different positions and may have different phases from each other. That is, the light generated at different positions of the filter unit 220 is different in phase, and the light generated at different positions of the light source unit 210 is classified as a color.

The photographing unit 230 receives a plurality of lights transmitted through the sample S to generate a plurality of phase information for the sample S, Obtain another image. Here, the different images denote the bright field image 10, the optical differential phase difference image 20, and the quantitative optical phase image 30, respectively.

The photographing unit 230 may include a color camera 231 and an operation unit 232.

The color camera 231 receives and photographs a plurality of light beams transmitted through the sample S and acquires images corresponding to the phase information of the lights transmitted through the sample S, respectively. More specifically, the color camera 231 may be a CCD camera or a CMOS camera. The color camera 231 may include three image sensors for detecting red light, green light, and blue light, respectively .

Since the plurality of light beams are received through the color camera 231 and are separable by color, the color information of the light sources can be separated at low cost to acquire images.

The arithmetic unit 232 performs arithmetic processing on the phase information corresponding to the plurality of lights through the image. Here, the process of calculating the phase information for the sample S through the operation unit 232 is the same as described above, and thus will not be described.

Meanwhile, the image acquisition system 200 may further include a guide unit 240 for guiding a plurality of lights output from the light source unit 210. The guide unit 240 includes a guide mirror 241 for guiding the paths of a plurality of lights output from the light source unit 210 and a guide mirror 241 for guiding the plurality of lights reflected by the guide mirror 241 to form images on the photographing unit 230 And a tube lens 242. Between the sample S and the photographing unit 230, an objective lens 250 for adjusting the magnification may be further provided.

7 is a view schematically showing a configuration of an image acquisition system according to another embodiment of the present invention. 8 is a diagram showing a comparative example of an area of light generated in the light source unit to be transmitted to the Fourier plane and an area of a region through which light defined in the Fourier plane can be transmitted, in FIG. In the present embodiment, differences from the above-described embodiment will be mainly described.

7 to 8, the image acquisition system 300 may be applied to a transmission type optical microscope and includes a light source unit 310, a filter unit 320, and a photographing unit 330.

The light source unit 310 may be an LED array that outputs white light. The filter unit 320 may be disposed on a Fourier plane (F). Here, the Fourier plane (F) refers to the plane on which the spectrum of the sample S is formed with respect to the spatial frequency.

The Fourier plane F in the present embodiment can be disposed on the guide portion 340. [ When the filter unit 320 is disposed on the Fourier plane F, the area L of light (light generated in the light source unit and transmitted to the Fourier plane) introduced into the Fourier plane F and the area L of the filter unit 320 The area (L) of the light incident on the Fourier plane (F) is equal to or larger than the area of the filter part 320 (the light defined by the Fourier plane is transmitted Is smaller than the area of the surface of the substrate.

This is because the area (L) of the light entering the Fourier plane (F) when the light (white light) emitted from the light source part 310 is reflected from the sample and then tilted due to the difference in refractive index with the periphery of the sample, The area L of the light incident on the Fourier plane F is smaller than the area of the filter 320 so that the difference in intensity of red, green and blue light occurs when the light is tilted. This is because the sensor can recognize it.

In other words, when the area L of the light (light emitted from the light source) that flows into the plane F is larger than the area of the filter unit 320, the information about the light tilted by the sample S I can lose it.

9 is a view schematically showing a configuration of an image acquisition system according to another embodiment of the present invention. In the present embodiment, differences from the above-described embodiment will be mainly described.

As shown in FIG. 9, the image acquisition system 400 includes a light source unit 410 and a photographing unit 420. Here, the image acquisition system 400 according to the present embodiment can be applied to a reflection type optical microscope.

The light source unit 410 has a plurality of color patterns 410a and simultaneously outputs a plurality of lights having different colors to the sample S through the color pattern 410a. That is, a plurality of lights output from the light source unit 410 are outputted at different positions and are formed to have different phases from each other. Specifically, the light source unit 410 may be configured to output red light, green light, and blue light, respectively, and each color pattern 410a may be divided evenly .

The light source unit 410 may include an LED array and a control unit.

The LED array outputs a plurality of lights having different colors. To this end, the LED array may include a plurality of light emitting diode arrays each of which is configured to emit beams of various colors. The light emitting diode array may be formed to output a plurality of lights having different colors through a known color pattern process.

The control unit is electrically connected to the LED array to change the brightness and color of the light output from the LED array.

The photographing unit 420 receives a plurality of lights reflected on the surface of the sample S to generate a plurality of phase information on the sample S and calculates a plurality of phase information on the sample S Obtain different images.

The photographing unit 420 may include a color camera 421 and an operation unit 422.

The color camera 421 receives and photographs a plurality of lights reflected on the surface of the sample S and acquires images corresponding to the phase information of the lights reflected on the surface of the sample S, respectively. More specifically, the color camera 421 may be a CCD camera or a CMOS camera. The color camera 421 may include three image sensors for detecting red light, green light, and blue light, respectively . Accordingly, it is possible to acquire each color image by receiving a plurality of lights through the image sensor.

The arithmetic operation unit 422 computes information of phases corresponding to the plurality of lights through an image. Here, the process of calculating the phase information for the sample S through the arithmetic unit 422 is the same as described above, and thus will not be described.

The image acquisition system 400 may further include a condenser 430 for condensing the light output from the light source unit 410 and irradiating the condensed light to the sample S. The condenser 430 is a kind of condenser lens and an objective lens 440 for adjusting magnification may be provided between the condenser 630 and the photographing unit 420.

A transmissive / reflective portion 450 may further be provided under the condenser 430, more specifically, between the condenser 430 and the objective lens 440. A part of the light having passed through the condenser 430 through the transmission / reflection part 450 is guided to the sample S and the light reflected from the sample S is reflected by the transmission / reflection part 450, (420).

10 is a view schematically showing a configuration of an image acquisition system according to another embodiment of the present invention. In the present embodiment, differences from the above-described embodiment will be mainly described.

As shown in FIG. 10, the image acquisition system 500 includes a light source unit 510, a filter unit 520, and a photographing unit 530. Here, the image acquisition system 500 according to the present embodiment can be applied to a reflection type optical microscope.

The light source unit 510 outputs light to the sample S. Here, the light source unit 510 may be formed of an LED array that outputs single color light, more preferably white light.

A pattern is formed in the filter unit 520 such that a plurality of different colors are imparted to the light output from the light source unit 510. Specifically, the filter unit 520 is a color filter for extracting three colors of red (R), green (G) and blue (B) from white light output from the light source unit Lt; / RTI >

The filter unit 520 is circular and divided into three equal widths, and the light passing through the divided filter unit 520 can be output as red light, green light, and blue light. In other words, the plurality of lights passing through the filter unit 520 may be outputted at different positions and may have different phases from each other. That is, the light generated at different positions of the filter unit 520 is different in phase, and the light generated at different positions of the light source unit 510 is classified as a color.

The photographing unit 530 receives the light reflected on the surface of the sample S to generate a plurality of pieces of phase information for the sample S and calculates a plurality of phase information for the sample S, Obtain the image. Here, the different images denote the bright field image 10, the optical differential phase difference image 20, and the quantitative optical phase image 30, respectively.

11 is a view schematically showing a configuration of an image acquisition system according to another embodiment of the present invention. In the present embodiment, differences from the above-described embodiment will be mainly described.

11, the image acquisition system 600 may be applied to a reflection type optical microscope, and includes a light source unit 610, a filter unit 620, and a photographing unit 630. Here, the light source unit 610 may be an LED array that outputs white light.

The image acquisition system 600 may further include a condenser 640 for condensing light output from the light source unit 610 and irradiating the condensed light to the sample S. The condenser 640 is a kind of condenser lens and an objective lens 650 for adjusting magnification may be provided between the condenser 640 and the photographing unit 630.

A transmissive / reflective portion 660 may further be provided under the condenser 640, more specifically between the condenser 640 and the objective lens 650. A part of the light having passed through the condenser 640 through the transmission / reflection part 660 is guided to the sample S and the light reflected from the sample S is reflected by the transmission / reflection part 660, 630 < / RTI >

The image acquisition system 600 may further include a guide unit 670 for guiding a plurality of lights having passed through the transmission / reflection unit 660 to the photographing unit 630. The guide portion 670 includes a reflection portion 671 for guiding a plurality of paths of light that have passed through the transmission / reflection portion 660, and a plurality of light beams that have passed through the reflection portion 671 are converged by the image pickup portion 630 And a tube lens 672 for guiding the light.

The guide unit 670 may further include a collecting lens 680 for collecting a plurality of lights reflected from the sample S. [ Here, the collecting lens 680 may be disposed between the guide portion 670 and the transmissive / reflective portion 660, and may be formed as a pair.

On the other hand, the filter unit 620 may be disposed on a Fourier plane (F). Here, the Fourier plane F is the distance between the guide 670, specifically between the second collecting lens 680 and the tube lens 672 in FIG. 11, or between the first collecting lens 680 and the objective lens 650 As shown in FIG. The area L of the light (light emitted from the light source) and the area of the filter 320 are equal to each other or the area L of the light entering the Fourier plane F Is formed to be smaller than the area of the filter unit 320 as described above with reference to FIGS.

As described above, the image acquisition system can acquire the bright field image, the optical differential phase difference image, and the quantitative optical phase image of the sample with only one photographing, so that the sample can be more precisely and accurately inspected.

Further, since it is possible to acquire the bright field image, the optical differential phase difference image, and the quantitative optical phase image with respect to the sample by only one photographing operation, it is possible to prevent the loss of motion information due to the time difference at the time of photographing, .

In addition, since red light, green light, and blue light are simultaneously output through only a low-cost LED array or filter, the initial installation cost can be reduced.

In addition, a bright field image, an optical differential phase difference image, and a quantitative optical phase image for the sample can be obtained by calculating the phase values of the lights obtained through the photographing unit. Therefore, since an expensive additional device such as a prism or an interference optical system is not required as in the prior art, the initial installation cost is reduced and the device can be easily implemented.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation and that those skilled in the art will recognize that various modifications and equivalent arrangements may be made therein. It will be possible. Accordingly, the true scope of protection of the present invention should be determined only by the appended claims.

110, 210, 310, 410, 510, 610,
110a, 410a .. color pattern
120, 230, 330, 420, 530, 630,
121, 231, 331, 421, 531, 631.
122, 232, 432, 422, 532, 632. [
130, 240, 340, 670,
140, 250, 350, 440, 550, 650. The objective lens
220, 320, 520, 620,

Claims (22)

1. An image acquisition system for acquiring an image of a sample,
A light source unit having a plurality of color patterns and simultaneously outputting a plurality of lights having different colors to the sample through the color pattern; And
A photographing unit that receives a plurality of lights transmitted through the sample to generate a plurality of phase information for the sample, and calculates a plurality of different images for the sample by calculating the phase information;
And an image acquisition system.
1. An image acquisition system for acquiring an image of a sample,
A light source unit having a plurality of color patterns and simultaneously outputting a plurality of lights having different colors to the sample through the color pattern; And
A photographing unit for receiving a plurality of lights reflected on a surface of the sample to generate a plurality of phase information for the sample and calculating a plurality of different images for the sample by calculating the phase information;
And an image acquisition system.
3. The method according to claim 1 or 2,
The light source unit includes:
An LED array for outputting a plurality of lights having different colors,
And a controller capable of changing the brightness and color of light output from the LED array.
3. The method according to claim 1 or 2,
Wherein the plurality of lights output from the light source unit are outputted at different positions and have different phases from each other.
3. The method according to claim 1 or 2,
Wherein the light source unit outputs red light, green light, and blue light, respectively.
3. The method according to claim 1 or 2,
Wherein the color pattern of the light source unit is divided equally.
3. The method according to claim 1 or 2,
Wherein,
A color camera for receiving and photographing a plurality of lights transmitted through the sample or reflected on the surface of the sample and acquiring images corresponding to phase information of the lights transmitted through the sample or reflected on the surface of the sample,
And an arithmetic unit for arithmetically processing information of a phase corresponding to each of the plurality of lights through the image.
8. The method of claim 7,
Wherein the color camera comprises a plurality of image sensors each for receiving the plurality of lights to acquire respective color images.
8. The method of claim 7,
Wherein the color camera is a CCD camera or a CMOS camera.
The method according to claim 1,
An objective lens disposed between the sample and the photographing unit,
And a guide unit for guiding a plurality of lights output from the light source unit.
11. The method of claim 10,
The guide portion
A guide mirror for guiding a path of a plurality of lights output from the light source unit,
And a tube lens for guiding a plurality of lights having passed through the guide mirror to form images with the photographing unit.
3. The method of claim 2,
A condenser for condensing the light outputted from the light source part and irradiating the light to the sample,
An objective lens disposed between the condenser and the photographing unit,
And a transmission / reflection unit provided between the condenser and the objective lens, for guiding a part of the light having passed through the condenser to the sample.
1. An image acquisition system for acquiring an image of a sample,
A light source for outputting light to the sample;
A filter unit having a plurality of colors different from each other in a light output from the light source unit; And
A photographing unit that receives a plurality of lights transmitted through the sample to generate a plurality of phase information for the sample, and calculates a plurality of different images for the sample by calculating the phase information;
And an image acquisition system.
1. An image acquisition system for acquiring an image of a sample,
A light source for outputting light to the sample;
A filter unit having a plurality of colors different from each other in a light output from the light source unit; And
A photographing unit for receiving light reflected on a surface of the sample to generate a plurality of phase information for the sample and calculating a plurality of different images for the sample by calculating the phase information;
And an image acquisition system.
The method according to claim 13 or 14,
Wherein the filter unit is disposed on the light source unit or on a Fourier plane.
The method according to claim 13 or 14,
Wherein the light source portion includes an LED array that outputs light of a single color.
The method according to claim 13 or 14,
Wherein light passing through the filter unit is output at different positions and has different phases from each other.
The method according to claim 13 or 14,
Wherein the filter unit is divided into three parts and the light having passed through the divided filter unit is output as red light, green light, and blue light, respectively.
14. The method of claim 13,
An objective lens disposed between the sample and the photographing unit,
And a guide unit for guiding a plurality of lights output from the light source unit.
20. The method of claim 19,
The guide portion
A guide mirror for guiding a path of a plurality of lights output from the light source unit,
And a tube lens for guiding a plurality of lights having passed through the guide mirror to form images with the photographing unit.
15. The method of claim 14,
A condenser for condensing the light outputted from the light source part and irradiating the light to the sample,
An objective lens disposed between the condenser and the photographing unit,
A transmission / reflection part provided between the condenser and the objective lens, for guiding a part of light having passed through the condenser to the sample,
And a guide unit for guiding a plurality of lights having passed through the transmission / reflection unit to the photographing unit.
22. The method of claim 21,
The guide portion
A reflecting portion for guiding a path of a plurality of light beams passed through the transmission / reflection portion,
And a tube lens for guiding the plurality of light beams passing through the reflection section to form images with the photographing section.

Images