KR20190022607A - A Cosmetic Composition for Relieving an Irritation from a Fine Particles - Google Patents

Abstract

The present invention relates to a cosmetic composition for relieving irritation caused by ultrafine particulate matters and, to be more specifically, a cosmetic composition for relieving irritation caused by ultrafine particulate matters, which can prevent and treat skin irritation caused by fine or ultrafine particulate matters included in the air in a dust form by antioxidant activities. In addition, the cosmetic composition for relieving irritation caused by ultrafine particulate matters comprises: substance 1 composed of vitamin E and quercetin; substance 2 composed of at least one selected as group 1 consisting of anthocyanin or proanthocyanin extracted from pine needles, epigallocatechin (EGCG), and flavonoid extracted from Caulerpa racemosa, at least one selected as group 2 consisting of beta-carotene or carotenoid, glutathione, and superoxide dismutase (SOD), and at least one selected as group 3 consisting of chitosan, alginic acid, beta-glucan, and gamma-PGA; and a solvent for making the whole composition to be 100 wt%.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for relieving ultrafine particle-induced irritation,

The present invention relates to a cosmetic composition for relieving ultrafine particle-induced irritation, and more particularly, to a cosmetic composition for preventing and / or improving skin irritation caused by fine or ultrafine particles contained in the air, To a cosmetic composition for alleviating particle-induced irritation.

Fine particulate matter generally refers to dust having a diameter of less than 2.5 μm, and may penetrate into the lungs of humans together with fine dust having a diameter of less than 10 μm, which may cause various respiratory diseases . Fine dust or ultrafine dust can be generated from fossil fuels such as automobile exhaust gas, and various regulations are being made to suppress the generation of such substances. Recent industrial developments have shown that nano-sized dust having a diameter smaller than that of ultrafine dust is generated, and such dust is deposited in the lungs to cause various types of diseases. These dusts are deposited in the lungs (In Panis, L. et al (2010), Atmosphere Environment 44: 2263-2270.), Oxidative stress (I. Romieu et al. : 179-197), or to damage organs, including the heart, by inducing an inflammatory response (L. Calderon-Garciduenas et al. (2008) Toxicology Pathology 36 (2): 289-310). These materials have also been reported to cause skin irritation by photooxidation (William J, Cosmetic and Toiletries, vol. 114, 75-82, 1999).

Generally, the photooxidation reaction occurs in various forms such as a reaction with free radicals bonded with oxygen, a reaction without free radical, an initial free radical generation reaction, or a late free radical generation reaction. Therefore, suppression of photooxidation by ultrafine dust needs to be done based on various types of photooxidation reactions. It is necessary to prevent the skin action of the ultrafine dust in order to suppress the photooxidation caused by the ultrafine dust, and to prevent the ultrafine dust from being removed when the skin action progresses and to suppress the progress of the oxidation reaction due to the ultrafine dust Means need to be used. For this purpose, it is necessary to use an antioxidant in various forms together with an oxygen scavenger which is capable of decomposing the hydroperoxide generated in the latter half of the reaction or inhibiting the formation, while suppressing the generation of initial free radicals. There is also a need to use means that can relieve irritation while simultaneously eliminating free radicals such as modulating cytokines or inhibiting phospholipases that produce inflammatory mediators. In addition, toothbrushing agents that adsorb ultrafine dust and block the action on the skin need to be used. Japanese Patent Laid-Open Publication No. JP08099820A discloses a method for preventing skin aging and inflammation by an antioxidant such as rutin and vitamin E as an external agent. However, it has a problem that it is difficult to cope with various types of stimulation by ultrafine dust due to the action mechanism of antioxidation. Patent Publication No. 10-2010-0056239 discloses a cosmetic composition containing at least one active ingredient selected from the group consisting of seaweed polysaccharides and ginseng polysaccharides and adsorbing heavy metals and fine dusts in dust, . Patent Publication No. 10-2016-0000959 discloses a cosmetic composition for removing heavy metals and fine dusts containing, as an active ingredient, a by-product produced in at least one fermentation process selected from distilled water, takju, fruit wine, sake, and Yakju .

The composition disclosed in the prior art is disadvantageous in that prevention of fine dust or heavy metal is difficult and removal of ultrafine dust is difficult.

The present invention has been made to solve the problems of the prior art and has the following purpose.

Prior Art 1: Japanese Laid-open Patent Application No. JP08099820A (published by Kosei Co., Ltd. on Apr. 16, 1996) Prior Art 2: Patent Publication No. 10-2010-0056239 (published by AMOREPACIFIC Corporation, May 27, 2010) Cosmetic composition for adsorbing heavy metals and fine dusts containing polysaccharides Prior Art 3: Patent Publication No. 10-2016-0000959 (published by Kuksundang Co., Ltd., Jan. 06, 2016) Cosmetic composition for removing heavy metals and fine dusts

It is an object of the present invention to provide a cosmetic composition for alleviating ultrafine particle-induced irritation, which has an effect on various skin actions of ultrafine dust.

According to a preferred embodiment of the present invention, the cosmetic composition for ultra-fine particle-induced stimulation relief comprises one component consisting of vitamin E and quercetin; (Beta-carotene or carotenoid; glutathione; and a superoxide dismutase (SOD)) selected from the group consisting of pine needle extract anthocyanin or proanthocyanin, epigallocatechin (EGCG) At least one member selected from the group consisting of at least one member selected from the group consisting of (chitosan, alginic acid, betaglucan, and gamma-PGA), and a solvent to make the whole 100 wt%.

According to another preferred embodiment of the present invention, the amount of the component belonging to each of the vitamin E, quercetin and the two components is 0.001 to 20.0 wt%.

According to another preferred embodiment of the present invention, at least a part of the flavonoid, proanthocyanin, epilocalocatein, beta-carotene or carotenoid is fermented.

The cosmetic composition according to the present invention makes it possible to protect the skin from heavy metals and ultrafine dusts which are generated in an industrial process or daily life, or are contained in the smoke of a cigarette or a car. The cosmetic composition according to the present invention is applied to the skin to form an effective skin protection method system without side effects. Specifically, the effect of the antioxidant raw material is exhibited by the mechanism including the antioxidant which acts in the early stage of the photoreaction and the late stage antioxidant, and the effect is exhibited on the ultrafine dust-induced photoreaction of various modes of action. The composition according to the present invention is applied to the skin to prevent the adsorption of heavy metals, thereby causing cytokine control, phospholipase inhibiting effect and delayed aging of skin cells.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the disclosed embodiments, but the present invention is not limited thereto.

The cosmetic composition according to the present invention comprises one component consisting of vitamin E and quercetin; (Beta-carotene or carotenoid; glutathione; and a superoxide dismutase (SOD)) selected from the group consisting of pine needle extract anthocyanin or proanthocyanin, epigallocatechin (EGCG) Two components consisting of at least one selected from the three groups consisting of at least one selected from the group consisting of (chitosan; alginic acid; betaglucan; and gamma-PGA), and a solvent such as water or glycerin which makes the whole 100 wt% .

In the cosmetic composition according to the present invention, the amount of the component belonging to each of the vitamin E, quercetin and the two components is 0.001 to 20.0 wt%.

Vitamin E may have a function of inhibiting the generation of free radicals generated in a later process of the photoreaction, and may be contained in an amount of 0.001 to 20 wt% based on the total weight. Quercetin may also have a function of regulating phospholipase while controlling the cytokine, and may be contained in an amount of 0.001 to 20 wt% of the total weight. The cosmetic composition according to the present invention may contain vitamin E and quercetin as essential components, and may optionally contain 1, 2 and 3 groups forming two components. In addition, the composition according to the present invention may contain vitamin E or a derivative thereof in place of vitamin E. It may also contain components such as coenzyme Q10. Coenzyme Q10 may be an additional ingredient and may be added in an amount ranging from 0.01 to 15% by weight based on the total composition weight.

Group 1 may contain pine needle extract anthocyanin or proanthocyanin, epicalocatechin (EGCG) and sea grape extract flavonoids to block the generation of free radicals that may occur in the initial stage of ultraviolet irradiation. Anthocyanin or proanthocyanin can be extracted from pine needles, flavonoids can be extracted from sea grapes, and epicalocateins can be extracted from green tea. And the anthocyanin or proanthocyanin, epicalocatechin and sea grape extracted flavonoids may be included in amounts of 0.001 to 20.0 wt%, respectively.

The two groups may contain beta-carotene, carotenoid, glutathione and a superoxide dismutase (SOD), which may occur in later stages of the photoreaction, and may be included in amounts of 0.001 to 20.0 wt%, respectively.

3 group may contain chitosan, alginic acid, betaglucan and gamma-PGA, each having an ability to adsorb ultrafine dust or having a removal performance, and may be contained in an amount of 0.001 to 20.0 wt%, respectively.

The composition according to the present invention may comprise two components including at least two groups from the group consisting of the first group, the second group and the third group. And may further comprise a solvent such as water or glycerin to bring the total to 100 wt%. If necessary, the composition according to the present invention may contain a cosmetic additive, and the present invention is not limited by the addition of such additional ingredients.

The composition according to the present invention may comprise at least a portion of the ingredients listed above as a fermented ingredient. For example, epicllo catechin corresponding to green tea extract, flavonoid corresponding to pine leaf extract, anthocyanin or proanthocyanin, and carotenoid may be added as a fermentation component. For fermentation, for example, Lactobacillus fermentum, Lactobacillus brevis, Lactobacillus plantarum, Aspergillus niger, Lactobacillus cellobionium, Lactobacillus spp. Bacteria such as Lactobacillus acidophilus or Lactobacillus delbrueckil can be used. The fermentation can be carried out at a temperature of 20 to 45 캜 for 1 to 4 days. Thereafter, the fermentation broth is centrifuged at 5,000 to 20,000 rpm for 5 to 40 minutes in a centrifuge, after which the supernatant solution can be separated and concentrated. Alternatively, pine needle or green tea can be solid fermented, and flavonoid, anthocyanin or proanthocyanin, or epicalocatein component can be obtained from the solid fermentation component. Specifically, the pine needle or green tea is washed and dried, and then the leaves of the dried component can be finely pulverized into powder form. Thereafter, it may be fermented in the MRS medium with lactobacillus bacteria or preferably with lactobacillus plantarum for 1 to 4 weeks. The fermentation can be carried out, for example, at 20 to 45 캜.

The fermentation component may be used, for example, in place of or entirely substitute for an anthocyanin or proanthocyanin, flavonoid or epicalocatechin component corresponding to a natural product.

The composition according to the present invention may contain various solvents known in the art, and may contain various functional ingredients as required.

In order to test the efficacy of the composition according to the invention, the composition according to the invention was prepared as follows.

<Examples> 1 group 2 groups 3 groups Sol TE GR EV β-Ca Glu SOD Ch Al beta -Gl γ-P One 20 10 10 57 2 10 15 10 10 52 3 15 15 0.009 10 57 4 10:10 10 10 57 5 5: 5 15 10 10 52 6 7: 8 15 0.009 10 57 7 Coenzyme Q10, which is 5% by weight based on the total weight, is added to Example 1

In the examples 1 to 7, TE is green tea, GR is sea berry, EV is pine leaf, beta-Ca is beta-carotene, Glu is glutathione, SOD is superoxide dismutase, Ch is chitosan, Gl represents beta-glucon, and gamma-P represents gamma-PGA. Examples 4, 5 and 6 show the weight ratio of the natural ingredient to the fermented ingredient, and the fermented ingredient was fermented with lactobacillus platelum at a temperature of 30 to 35 DEG C for 2 days The efficacy was tested for Examples 1-7.

result

1. cytokine modulating effect

The skin cells were treated with heavy metals, and the skin was biopsied 18 hours after the irradiation, and a 20-fold volume of potassium phosphate saline buffer buffer was added to make it homogeneous. The supernatant solution was then obtained by centrifuging the solution at 15,000 rpm for 5 minutes. Then, the RNA (ribonucleic acid) was separated into TRIZOL reagent and subjected to electrophoresis to perform only RT-PCR. RT-PCR was performed by reverse transcription using reverse transcriptase, and PCR reaction (gene specific amplification reaction) was performed on IL-1 and IL-10, respectively. The cytokine regulation experiment on the animals was carried out according to the method described in Clin. Exp. Immunol., Vol. 120 (No 1), p 213-223 (2000).

The degree of expression of the stimulus was compared with the case where no heavy metal of 100 ppm was treated (non-treated group) and the case where only heavy metal as a cause of stimulation was treated and when each sample was treated together. Expression ratio was obtained by the following equation.

(%) = (Value at the time of inclusion of the test substance-blank value) * 100 / blank value

In this case, the average value of the non-treatment group (blank) corresponding to the absence of the test substance in the above formula was set to 100. In this case, the error rate of the IL-1 expression was 3.35, The error was ± 2.75. After the PCR reaction, each was subjected to agarose electrophoresis and its value was corrected by densitometry to the value of internal standard substance GAPDH (Glyceraldehyde 3-phosphate dehydrogenase).

<Modulation effect of cytokine by heavy metal treatment of cells> Test substance IL-1 (mean value / error) IL-10 (mean value / error) heavy metal 200.5 / 4.35 215 / 3.86 Heavy metal + 0.1% quercetin 125 / 2.37 127 / 2.43 Heavy metal + pine needle OPC 0.1% 128 / 2.45 137 / 3.72 Heavy metal + sea grape extract 0.1% 140 / 1.36 143 / 4.54 Heavy Metal + Gamma Fiji AA 0.1% 176 / 3.21 156 / 3.48 Untreated group 100 / 3.35 100 / 2.75

As shown in the results, the expression of IL-1 and IL-10 is increased by heavy metals, but their expression is decreased by quercetin, pine needle anthocyanin, sea grape extract, gamma-peji and such flavonoids. 2. Removal of Heavy Metals Lead (100 ppm) and cadmium (100 ppm) solutions were reacted with each of Examples 1 to 7 for 1 hour and then filtered. The heavy metal content of the remaining solution was measured by ICP-OES Respectively. As a control group, a group containing only purified water was used. Adsorption ratios were calculated by dividing the concentration of heavy metals and adsorbed sample by the concentration of the control sample. The higher the adsorption ratio, the more effective adsorption and removal of heavy metals. The removal rate of the heavy metal was calculated as (1 - adsorption ratio) * 100, and the results are shown below.

&Lt; Heavy metal removal effect & Example Lead Removal Rate (%) Cadmium removal rate (%) One 75.8 80.4 2 76.9 82.3 3 77.1 82.1 4 81.2 84.4 5 87.9 89.2 6 88.7 90.1 7 80.2 82.3 Control group 0 0

3. Prevention of Heavy Metals The cream of the example is applied to pig skin (2 cm * 2 cm), placed on a square acrylic box of 30 cm in height and 30 cm in height, and sprayed with a liquid containing heavy metals to be applied to the skin. And a case in which the cream containing no ingredient was used as a negative control.

The removal rate of the heavy metal was calculated as (1 - adsorption ratio) * 100, and the results are shown below.

<Heavy metal prevention effect> Example Lead adsorption (%) Cadmium removal rate (%) One 1.5 2.8 2 2.1 2.9 3 3.1 3.5 4 1.1 1.9 5 1.3 2.2 6 1.5 2.3 7 1.3 2.5 Control group 88.5 82.1

4. Skin protection effect Human-derived keratinocytes were plated at a concentration of 1 * 10 ⁴ per well in a 96-well plate incubator, cultured for 24 hours, treated with ultrafine dust solution containing 100 ppm each of lead and cadmium Treated with purified water containing no lead and cadmium as the negative control group and treated with the ultrafine dust as the positive control group.

<Skin protection effect> Example Cell viability (100 ppm lead) (%) Cell viability (100 ppm cadmium) (%) One 90.5 91.2 2 89.4 90.8 3 88.6 90.2 4 91.3 92.8 5 91.8 93.1 6 92.1 93.4 7 93.3 92.9 Control group 40.2 41.1

As can be seen from the results, the cosmetic composition according to the present invention makes it possible to protect the skin from heavy metals and ultrafine dusts which are generated in an industrialization process or daily life, or are contained in smoke of a car or the smoke of a car. The cosmetic composition according to the present invention is applied to the skin to form an effective skin protection method system without side effects. Specifically, the effect of the antioxidant raw material is exhibited by the mechanism including the antioxidant which acts in the early stage of the photoreaction and the late stage antioxidant, and the effect is exhibited on the ultrafine dust-induced photoreaction of various modes of action. The composition according to the present invention can be applied to the skin to prevent the adsorption of heavy metals, thereby causing cytokine control, phospholipase inhibiting effect, and delayed aging of skin cells. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. The invention is not limited by these variations and modifications, but is limited only by the claims appended hereto.

Claims (1)

0.001 to 20.0 wt% of one component consisting of vitamin E and quercetin;
(Beta-carotene or carotenoids; glutathione; and a superoxide dismutase (SOD)) selected from the group consisting of atropine (proanthocyanin, epigallocatechin (EGCG), and sea grape extract flavonoid) (Chitosan; alginic acid; beta-glucan and gamma-PGA); and at least one of the following two components:
0.01 wt% to 15 wt% coenzyme Q10; And
A solvent to make the whole 100 wt%
The flavonoid, proanthocyanin or epicalocatechin is a fermentation product obtained by fermenting an extract containing flavonoid, proanthocyanin or epicalocatechin, and the group 3 for adsorption or removal of ultrafine dust is 10 to 20 wt% , Quercetin, sea grape extracted flavonoids or gamma-peptides, whereby the cytokine is regulated.