KR20190017137A - Portable oxygen generator using catalase-immobilized alginic acid bead - Google Patents

Portable oxygen generator using catalase-immobilized alginic acid bead Download PDF

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KR20190017137A
KR20190017137A KR1020170101481A KR20170101481A KR20190017137A KR 20190017137 A KR20190017137 A KR 20190017137A KR 1020170101481 A KR1020170101481 A KR 1020170101481A KR 20170101481 A KR20170101481 A KR 20170101481A KR 20190017137 A KR20190017137 A KR 20190017137A
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hydrogen peroxide
oxygen
catalase
water
control valve
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김홍기
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김홍기
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    • C01B13/00—Oxygen; Ozone; Oxides or hydroxides in general
    • C01B13/02—Preparation of oxygen
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01—Peroxidases (1.11.1)
    • C12Y111/01006—Catalase (1.11.1.6)

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Abstract

The present invention relates to a portable oxygen generator comprising a hydrogen peroxide decomposition vessel, in which alginic acid beads, on which catalase for decomposing hydrogen peroxide to generate oxygen is immobilized, are filled. The oxygen generator of the present invention is superior in safety to a chemical oxygen generator by using catalase which is an enzyme protein to decompose hydrogen peroxide, and stability and reusability of the enzyme are increased by immobilizing the catalase to the alginic acid beads.

Description

카탈라제가 고정화된 알긴산 비드를 이용한 휴대용 산소발생장치{Portable oxygen generator using catalase-immobilized alginic acid bead}TECHNICAL FIELD [0001] The present invention relates to a portable oxygen generator using catalase-immobilized alginic acid beads,

본 발명은 카탈라제가 고정화된 알긴산 비드를 이용한 휴대용 산소발생장치에 관한 것이다.The present invention relates to a portable oxygen generator using catalase-immobilized alginic acid beads.

최근 대기 오염이 심해지고, 냉난방기의 보급에 따라 밀폐된 공간에서 주거하는 시간이 많아졌으며, 건강에 대한 관심이 증가함에 따라, 산소발생장치가 주목을 받고 있다. 현재 산소를 공급하기 위해 일반적으로 사용되는 방법은 고압산소탱크, 액화산소, 산소농축장치 등이 이용되고 있으며, 산소저장장치는 고압의 기체 혹은 액체 상태의 산소를 보관하여 사용하는 방식이며, 산소농축장치의 경우 공기 중의 질소를 산소로부터 분리하는 질소분리막을 사용하는 방식이다.Recently, as the air pollution becomes worse and the supply of the air conditioner becomes more and more time to stay in the closed space, and the interest in health increases, the oxygen generating apparatus is getting attention. Currently, high pressure oxygen tanks, liquefied oxygen, and oxygen concentration devices are commonly used to supply oxygen. Oxygen storage devices are used to store high pressure gas or liquid oxygen, In the case of the apparatus, a nitrogen separator for separating nitrogen in the air from oxygen is used.

고압산소 및 산소농축장치 이외에 특히 항공기의 경우, 무기초과산화물(inorganic superoxide), 염소산염(chlorate), 과염소산염(perchlorate), 오존화물(ozonide)로부터 화학반응을 통해 현장에서 산소를 발생시키는 장치를 사용하고 있다. 초산화칼륨(potassium superoxide)은 소비에트연방의 유인우주선에서 사용되었고 소방관이나 탄광구조대의 산소공급을 위한 장치로 이용된 바 있다. 그러나, 산소 발생 반응은 발열반응이라 화학적 산소발생장치는 화재의 우려가 있다. 항공기의 경우 조종사와 승무원들은 산소통을 지급받지만 여객칸에서 비상시에 내려오는 산소호흡기에는 염소산나트륨(sodium chlorate)으로부터 화학적으로 발생하는 산소가 공급되는데, 이 화학반응은 매우 격렬하게 진행되어 온도가 260℃까지 상승하게 된다. 실제로 화학적 산소발생기로부터 발생한 화재로 인해 다수의 항공기 화재 및 추락사고가 보고되었다. 따라서, 보다 안전하고 효율적이며 환경친화적인 산소발생 및 공급장치의 개발이 요구되고 있는 실정이다.In addition to the high-pressure oxygen and oxygen concentrators, in particular for aircraft, a device that generates oxygen in the field through chemical reactions from inorganic superoxide, chlorate, perchlorate, and ozone is used . Potassium superoxide has been used in manned spacecraft in the Soviet Union and has been used as a device for oxygen supply to firefighters and coal mines. However, since the oxygen generating reaction is an exothermic reaction, the chemical oxygen generating apparatus may cause a fire. In the case of airplanes, pilots and crews receive oxygen cigarettes, but oxygen from the sodium chlorate is supplied to the oxygen respiratory system in the passenger compartment. This chemical reaction is very intense and the temperature is 260 ° C . In fact, a number of aircraft fires and falls have been reported due to fires from chemical oxygen generators. Therefore, there is a demand for development of safer, more efficient and environmentally friendly oxygen generating and supplying apparatuses.

한편, 한국공개특허 제2016-0110931호에는 '산소발생기'가 개시되어 있고, 한국등록특허 제0773121호에는 '과산화수소 분해기 및 이것을 이용한 산소발생기'를 개시하고 있으나, 본 발명의 카탈라제가 고정화된 알긴산 비드를 이용한 휴대용 산소발생장치에 대해서는 기재된 바가 없다.Korean Patent Laid-Open Publication No. 2016-0110931 discloses an oxygen generator, Korean Patent No. 0773121 discloses a hydrogen peroxide decomposer and an oxygen generator using the hydrogen peroxide decomposer. However, since the catalase of the present invention contains alginic acid beads There has been no description of a portable oxygen generating device using the same.

본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명자는 과산화수소의 분해를 통한 산소발생에 있어서, 카탈라제 효소를 반응 촉매로 사용하였고, 상기 효소의 안정성 및 재사용성 증가를 위해 칼슘알긴산 겔에 상기 효소를 고정화함으로써, 과산화수소로부터 지속적인 산소발생의 효과를 확인함으로써, 본 발명을 완성하였다.DISCLOSURE OF THE INVENTION The present invention has been made in view of the above-mentioned needs. The present inventors have found that, in the generation of oxygen through the decomposition of hydrogen peroxide, the catalase enzyme is used as a reaction catalyst, and in order to increase the stability and reusability of the enzyme, By confirming the effect of continuous oxygen generation from hydrogen peroxide by immobilizing the enzyme, the present invention has been completed.

상기 과제를 해결하기 위해, 본 발명은 카탈라제(catalase) 효소가 고정화된 칼슘알긴산 비드가 내부에 충전된 과산화수소 분해조를 포함하는 휴대용 산소발생장치를 제공한다.In order to solve the above problems, the present invention provides a portable oxygen generator comprising a hydrogen peroxide decomposition tank filled with calcium alginate beads immobilized with a catalase enzyme.

또한, 본 발명은 상기 산소발생장치를 이용하여 산소를 발생시키는 방법을 제공한다.The present invention also provides a method for generating oxygen using the oxygen generating apparatus.

본 발명의 산소발생장치는 과산화수소를 분해하여 산소를 생성함으로써, 산소발생기가 컴팩트해지는 장점이 있으며, 과산화수소를 분해하기 위해 효소 단백질인 카탈라제를 사용함으로써 화학적 산소발생장치에 비해 안전성이 우수하다. 또한, 상기 카탈라제를 알긴산 비드에 고정함으로써, 효소의 안정성 및 재사용성이 증가될 수 있어, 소형의 저가의 산소발생장치를 제공할 수 있을 것이다.The oxygen generator of the present invention is advantageous in that the oxygen generator is compact by decomposing hydrogen peroxide to generate oxygen, and the enzyme protein CATALASE is used to decompose hydrogen peroxide, which is superior to the chemical oxygen generator. In addition, by fixing the catalase to alginic acid beads, the stability and reusability of the enzyme can be increased, and a small-sized, low-cost oxygen generator can be provided.

도 1은 본 발명의 휴대용 산소발생장치의 모식도이다.
도 2는 과산화수소수에 카탈라제를 첨가하고 산소 발생 여부를 확인한 사진이다.
도 3은 카탈라제가 고정화된 알긴산 비드를 제조하는 방법 및 이를 이용한 산소발생 과정의 모식도(a)와, 실제 제조된 카탈라제가 고정화된 알긴산 비드의 모습을 보여주는 사진(b)이다.
도 4는 과산화수소에 카탈라제가 고정화된 알긴산 비드를 첨가하고 산소 발생 여부를 확인한 사진이다.1 is a schematic diagram of a portable oxygen generator of the present invention.
Fig. 2 is a photograph showing catalase added to hydrogen peroxide water to confirm whether or not oxygen is generated. Fig.
FIG. 3 is a schematic view (a) of a process for producing alginic acid beads immobilized with catalase and an oxygen generating process using the same, and a photograph (b) showing the appearance of actually prepared catalase-immobilized alginate beads.
FIG. 4 is a photograph showing addition of catalase-immobilized alginic acid beads to hydrogen peroxide to confirm whether or not oxygen is generated.

본 발명의 목적을 달성하기 위하여, 본 발명은 카탈라제(catalase) 효소가 고정화된 칼슘알긴산 비드가 내부에 충전된 과산화수소 분해조를 포함하는 휴대용 산소발생장치를 제공한다.In order to accomplish the object of the present invention, the present invention provides a portable oxygen generator comprising a hydrogen peroxide decomposition tank filled with calcium alginate beads immobilized with a catalase enzyme.

이하에서는 도면을 참조하여 실시예에 대해서 구체적으로 설명하기로 한다.Hereinafter, embodiments will be described in detail with reference to the drawings.

도 1은 본 발명의 휴대용 산소발생장치의 모식도로, 본 발명의 과산화수소수로부터 산소기체를 발생시키는 산소발생장치(10)는, 일측은 과산화수소수를 공급받을 수 있는 주입관(21)이 연결되며 타측은 과산화수소수의 유입을 조절하는 제 1 조절밸브(15)와 연결된, 과산화수소수 저장조(11); 상기 제 1 조절밸브(15)와 일측이 연결되며 타측은 물의 배출을 조절하는 제 2 조절밸브(16)와 연결되고, 상단면에는 생성된 산소기체를 배출할 수 있는 산소배출관(18)이 연결된, 과산화수소수를 산소기체와 물로 분해하기 위한 과산화수소 분해조(12); 및 상기 제 2 조절밸브(16)와 일측이 연결되며, 타측에는 물을 배출할 수 있는 배출관(22)이 연결된, 과산화수소수 분해에 의해 발생한 물을 분리하기 위한 물 저장조(13);를 포함하는 것을 특징으로 하는 휴대용 산소발생장치를 제공한다.FIG. 1 is a schematic view of a portable oxygen generator of the present invention. In the oxygen generator 10 for generating oxygen gas from hydrogen peroxide water of the present invention, an injection pipe 21 capable of receiving hydrogen peroxide water is connected to one side And the other side is connected to a first control valve (15) for regulating the inflow of hydrogen peroxide water; a hydrogen peroxide water storage tank (11); The first control valve 15 is connected to the first control valve 15 and the other is connected to the second control valve 16 for controlling the discharge of water. The oxygen discharge pipe 18 for discharging the generated oxygen gas is connected to the upper surface of the second control valve 16 A hydrogen peroxide decomposition tank 12 for decomposing hydrogen peroxide water into oxygen gas and water; And a water storage tank (13) for separating water generated by the hydrogen peroxide decomposition, one end of which is connected to the second control valve (16) and a discharge pipe (22) Wherein the oxygen-containing gas is supplied to the oxygen-containing gas supply unit.

또한, 본 발명의 상기 과산화수소 분해조(12)에 연결된 산소배출관(18)은 과산화수소 분해조와 직접적으로 연결될 수도 있고, 또는 과산화수소 분해조와 산소배출관을 서로 연결하는 연결관(14)을 통해 연결될 수도 있으나, 이에 제한되지 않는다.The oxygen discharge pipe 18 connected to the hydrogen peroxide decomposition tank 12 of the present invention may be directly connected to the hydrogen peroxide decomposition tank or may be connected through the connection pipe 14 connecting the hydrogen peroxide decomposition tank and the oxygen discharge pipe, But is not limited thereto.

본 발명의 산소발생장치에 있어서, 상기 과산화수소 분해조(12)는 카탈라제(catalase) 효소가 고정화된 알긴산 비드(17)가 내부에 충전되어 구비된 것으로, 카탈라제는 과산화수소를 분해하여 산소기체와 물을 발생시키는 촉매로 사용되었다. 효소 촉매는 낮은 온도 및 낮은 압력에서 효과적으로 과산화수소 분해 반응을 촉매하므로, 화학적 산소발생장치에 비해 안정하게 산소를 공급할 수 있는 장점이 있다.In the oxygen generator of the present invention, the hydrogen peroxide decomposition tank 12 is provided with an alginic acid bead 17 filled with catalase enzyme immobilized therein, and catalase decomposes hydrogen peroxide into oxygen gas and water Lt; / RTI > Since the enzyme catalyst effectively catalyzes the hydrogen peroxide decomposition reaction at low temperature and low pressure, it has an advantage that it can supply oxygen more stably than the chemical oxygen generating apparatus.

그러나, 100% 과산화수소에 카탈라제를 첨가한 경우, 처음 1분 이내에는 급격한 산소발생이 관찰되었으나, 카탈라제 첨가 2분 후에는 더 이상의 산소발생이 일어나지 않음이 관찰되었다(도 2). 이는 과산화수소에 의한 카탈라제의 불활성에 의한 것으로, 지속적인 산소발생을 위해 과산화수소를 순차적으로 희석하여 테스트한 결과, 10%(v/v)의 과산화수소수에서 30분 이상 지속적으로 산소가 발생되는 것을 확인하였다. 또한, 카탈라제를 과산화수소수에 그대로 첨가할 경우 과산화수소에 녹아있는 카탈라제의 재사용이 어렵고, 효소의 안정성을 유지하기 어렵다는 문제점이 있다. 따라서, 본 발명자는 칼슘알긴산 겔에 카탈라제를 고정화하여 효소가 고정화된 알긴산 비드를 제조하였다(도 3). However, when catalase was added to 100% hydrogen peroxide, abrupt oxygen evolution was observed within the first minute, but no further oxygen evolution occurred after 2 minutes of catalase addition (FIG. 2). This was due to the inactivation of catalase by hydrogen peroxide. As a result of successive dilution of hydrogen peroxide for continuous oxygen production, it was confirmed that oxygen was continuously generated for 10 minutes at a concentration of 10% (v / v) of hydrogen peroxide over 30 minutes. In addition, when catalase is added to the hydrogen peroxide solution as it is, it is difficult to reuse the catalase dissolved in hydrogen peroxide, and it is difficult to maintain the stability of the enzyme. Therefore, the present inventors immobilized catalase on calcium alginate gel to prepare alginic acid beads immobilized with enzyme (FIG. 3).

알긴산나트륨은 시그마사에서 구입하여 4%(w/v) 농도가 되도록 증류수에 녹여 준비하였다. 그 후, 카탈라제(시그마, cat.# C9322)를 1 g/ℓ의 농도로 증류수에 희석하여 상기 알긴산나트륨 용액과 1:1로 혼합하고, 혼합물을 12 gauge 크기의 구멍 크기를 갖는 주사기에 옮긴 후, 100mM 농도의 염화칼슘 용액에 적하(dripping)하여 카탈라제 효소가 고정화된 알긴산 비드를 제조하였다.Sodium alginate was purchased from Sigma and dissolved in distilled water to a concentration of 4% (w / v). Thereafter, catalase (Sigma, cat. # C9322) was diluted in distilled water to a concentration of 1 g / l, mixed with the sodium alginate solution 1: 1 and the mixture was transferred to a syringe having a pore size of 12 gauge , And dripped into a calcium chloride solution at a concentration of 100 mM to prepare alginic acid beads immobilized with catalase enzyme.

제조된 카탈라제가 고정화된 알긴산 비드를 10% 과산화수소수에 첨가한 결과, 10분 이상 지속적으로 산소기체가 발생함을 확인할 수 있었다(도 4). 또한, 반응에 사용한 알긴산 비드를 수거하여 신선한 10% 과산화수소수에 재첨가한 결과에서도 산소기체가 10분 이상 지속적으로 발생함을 확인할 수 있었다.The prepared catalase-immobilized alginate beads were added to 10% aqueous hydrogen peroxide solution, and it was confirmed that oxygen gas was continuously generated for 10 minutes or more (FIG. 4). Also, it was confirmed that the alginic acid beads used in the reaction were collected and added to fresh 10% hydrogen peroxide water continuously for 10 minutes or more.

본 발명의 휴대용 산소발생장치에 있어서, 과산화수소수 저장조(11)에 저장되는 과산화수소수는 원액 또는 희석된 과산화수소수일 수 있고, 바람직하게는 5~15%(v/v) 농도의 희석된 과산화수소수일 수 있으나, 이에 제한되지 않는다. 과산화수소수는 강한 산화력을 가지고 있으며 강한 자극성이 있으므로, 5~15%(v/v) 농도의 희석된 과산화수소수를 사용하는 본 발명의 산소발생장치는 원액의 과산화수소수 사용에 따른 위험성이 경감된, 안전성이 우수한 장치이다.In the portable oxygen generator of the present invention, the hydrogen peroxide solution stored in the hydrogen peroxide solution storage tank 11 may be the undiluted solution or diluted hydrogen peroxide solution, and may preferably be a diluted hydrogen peroxide solution at a concentration of 5 to 15% (v / v) But is not limited thereto. Since the hydrogen peroxide water has a strong oxidizing power and is strongly irritant, the oxygen generating apparatus of the present invention using diluted hydrogen peroxide water at a concentration of 5 to 15% (v / v) It is an excellent safety device.

본 발명의 휴대용 산소발생장치에 있어서, 제 1 조절밸브(15)는 산소발생이 필요하지 않을 경우, 카탈라제가 고정화된 알긴산 비드가 충전된 과산화수소 분해조(12)와 과산화수소의 혼합을 방지하는 것으로, 산소발생이 필요한 경우에 과산화수소수 저장조(11)에 보관되어 있던 과산화수소수를 분해조(12)로 유입시키는 기능을 수행한다. 또한, 제 2 조절밸브(16)는 산소발생이 요구되는 상황에서 과산화수소 분해조(12)내에서 카탈라제에 의한 과산화수소의 분해 반응 결과 생성된 물을 배출하기 위한 것으로, 반응이 완료된 과산화수소 분해조(12)로부터 물을 배출시키는 기능을 수행한다. 상기 제 1 조절밸브와 제 2 조절밸브는 각각 당업계의 공지된 기술을 통해 다양한 형태로 제조할 수 있고, 조절 방식 또한 다양한 방법으로 이루어질 수 있다.In the portable oxygen generator of the present invention, the first control valve 15 prevents mixing of hydrogen peroxide with the hydrogen peroxide decomposition tank 12 filled with alginic acid beads to which catalase is immobilized when oxygen generation is not required, And performs the function of introducing the hydrogen peroxide solution stored in the hydrogen peroxide solution storage tank 11 into the decomposition tank 12 when oxygen generation is required. The second control valve 16 is for discharging the water produced as a result of the decomposition reaction of hydrogen peroxide by the catalase in the hydrogen peroxide decomposition tank 12 in a situation where oxygen generation is required. The hydrogen peroxide decomposition tank 12 To discharge the water. The first regulating valve and the second regulating valve may be manufactured in various forms through known techniques in the art, and the regulating method may also be performed by various methods.

본 발명은 또한, 상기 휴대용 산소발생장치를 이용하여 산소를 발생시키는 방법을 제공한다. 본 발명의 휴대용 산소발생장치는 과산화수소와 카탈라제를 이용하여 산소를 발생시키는 것으로, 휴대용 산소발생장치 내부에 과산화수소를 저장하는 저장조와, 산소발생 반응의 지속성 및 재연성을 위해, 카탈라제 효소 고정화된 알긴산 비드가 내부에 충진된 분해조로 구비되어 있어, 안정적이고 지속적으로 산소를 발생할 수 있다.The present invention also provides a method for generating oxygen using the portable oxygen generator. The portable oxygen generator of the present invention generates oxygen by using hydrogen peroxide and catalase, and comprises a storage tank for storing hydrogen peroxide in the portable oxygen generator, and a storage tank for storing the hydrogen peroxide and the algae beads immobilized with catalase enzyme It is equipped with a decomposition tank filled in the inside, and can generate oxygen stably and continuously.

10 : 산소발생장치 16 : 제 2 조절밸브
11 : 과산화수소수 저장조 17 : 카탈라제 효소가 고정화된 알긴산 비드
12 : 과산화수소 분해조 18 : 산소배출관
13 : 물 저장조 21 : 주입관
14 : 연결관 22 : 배출관
15 : 제 1 조절밸브10: oxygen generator 16: second control valve
11: hydrogen peroxide water storage tank 17: alginic acid beads immobilized with catalase enzyme
12: hydrogen peroxide decomposition tank 18: oxygen discharge pipe
13: Water reservoir 21: Injection pipe
14: Connector 22: Discharge tube
15: first control valve

Claims (4)

과산화수소수로부터 산소기체를 발생시키는 산소발생장치에 있어서, 일측은 과산화수소수를 공급받을 수 있는 주입관이 연결되며 타측은 과산화수소수의 유입을 조절하는 제 1 조절밸브와 연결된, 과산화수소수 저장조; 상기 제 1 조절밸브와 일측이 연결되며 타측은 물의 배출을 조절하는 제 2 조절밸브와 연결되고, 상단면에는 생성된 산소기체를 배출할 수 있는 산소배출관이 연결된, 과산화수소수를 산소기체와 물로 분해하기 위한 과산화수소 분해조; 및 상기 제 2 조절밸브와 일측이 연결되며, 타측에는 물을 배출할 수 있는 배출관이 연결된, 과산화수소수 분해에 의해 발생한 물을 분리하기 위한 물 저장조를 포함하는 것을 특징으로 하는 휴대용 산소발생장치.An oxygen generator for generating oxygen gas from hydrogen peroxide water, comprising: a hydrogen peroxide solution storage tank connected to a first control valve, one end of which is connected to an inlet pipe capable of receiving hydrogen peroxide solution, and the other of which is connected to a first control valve for regulating inflow of hydrogen peroxide solution; The first control valve is connected to one side of the first control valve and the other side is connected to a second control valve for controlling the discharge of water, and an oxygen exhaust pipe for discharging the generated oxygen gas is connected to the upper surface, and hydrogen peroxide water is decomposed into oxygen gas and water A hydrogen peroxide decomposition tank; And a water storage tank connected to one side of the second control valve and connected to a discharge pipe for discharging water on the other side, for separating water generated by hydrogen peroxide decomposition. 제1항에 있어서, 상기 과산화수소 분해조는 카탈라제(catalase) 효소가 고정화된 알긴산 비드가 내부에 충전된 것을 특징으로 하는 휴대용 산소발생장치.The portable oxygen generator according to claim 1, wherein the hydrogen peroxide decomposition tank is filled with alginic acid beads immobilized with a catalase enzyme. 제1항에 있어서, 상기 과산화수소수는 5~15%(v/v)의 농도인 것을 특징으로 하는 휴대용 산소발생장치.The portable oxygen generator according to claim 1, wherein the hydrogen peroxide solution has a concentration of 5 to 15% (v / v). 제1항 내지 제3항 중 어느 한 항의 산소발생장치를 이용하여 산소를 발생시키는 방법.A method for generating oxygen using the oxygen generating apparatus according to any one of claims 1 to 3.
KR1020170101481A 2017-08-10 2017-08-10 Portable oxygen generator using catalase-immobilized alginic acid bead KR20190017137A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021101728A1 (en) * 2019-11-18 2021-05-27 University Of Florida Research Foundation Modular, oxygen-generating microbead materials for supporting cell viability and function

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021101728A1 (en) * 2019-11-18 2021-05-27 University Of Florida Research Foundation Modular, oxygen-generating microbead materials for supporting cell viability and function

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