KR20190013131A - Apparatus for measuring bioelectric signals of micro-animals and method for measuring the same - Google Patents

Abstract

The present invention relates to a biometric signal measuring apparatus of a micro animal and a measuring method thereof. More specifically, the present invention relates to the biometric signal measuring apparatus which can quickly measure a biometric signal by simply and quickly fixing a micro animal while maintaining a life of a micro animal, and the method thereof. The biometric signal measuring apparatus comprises: a board placing a micro animal at an upper part; a fixing fluid receiving the micro animal in an inner space to make the micro animal to be fixed in a state of being placed on the board; and a needle electrode inserted into the micro animal received in the inner fixing fluid and measuring a biometric signal of the micro animal. The biometric signal measuring apparatus can be provided to make the micro animal to be fixed on the board by surface tension of the fixing fluid.

Description

TECHNICAL FIELD [0001] The present invention relates to an apparatus for measuring biological signals of a micro-animal and a method of measuring the same.

The present invention relates to an apparatus for measuring a biological signal of a micro-animal and a method of measuring the same, and more particularly, to a micro-animal signal measuring apparatus and method for measuring a biological signal of a micro- The present invention relates to an apparatus for measuring a biological signal of an animal and a method of measuring the same.

In general, EMG (Electromyography) is an examination method for evaluating the function of muscles by recording the activity potential generated during contraction of the muscle fiber. The EMG is a method of detecting the electrical change of the muscle in response to the nerve stimulation. Muscles are under the control of the nerves and the microcurrent is always flowing through the muscles themselves, so if you record them on an electromyographic device with a needle or an electrode, you can see whether the muscles are functioning properly from the peripheral nerve to the muscle itself. Therefore, electromyography is mainly used in cases of peripheral nerve and muscle problems, and is particularly used to detect muscular conditions that lead to muscle weakness such as muscle atrophy and neuropathy.

Recently, techniques for performing tests such as EMG, EEG, ECG and the like on micro-animals similar to human genetic information have been developed. As an example, a system is being developed for performing electromyography on Zebrafish, a vertebrate animal similar to human genetic information in microalgae.

However, it has been difficult to simultaneously measure microalgae in an EMG-based electromyography test system for microalgae, and it has been troublesome to insert microalgae into an agarose gel or a coil electrode and fix them.

Japanese Patent Application Laid-Open No. 2002-085362

An object of the present invention to solve the above-mentioned problems is to provide a device for measuring biological signals of micro-animals and a method for measuring biological signals of the micro-animals, in order to rapidly measure biological signals by fast and easy fixing of micro- Method.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise form disclosed. There will be.

According to an aspect of the present invention, there is provided a microfluidic device including: a substrate on which a micro-animal is placed; A fixer for accommodating the micro-animal in an inner space so that the micro-animal is fixed on the substrate; And a needle electrode inserted into the micro-animal accommodated in the fixation fluid and measuring a bio-signal of the micro-animal, wherein the micro-animal is fixed to the substrate by surface tension of the fixative. A biological signal measurement device is provided.

In an embodiment of the present invention, the substrate is made of a first comparison electrode, and when the needle electrode is inserted into the micro-animal, a potential difference between the needle electrode and the first comparison electrode is measured, And a signal is measured.

In an embodiment of the present invention, the substrate may further include a second comparison electrode provided on one surface of the substrate, and the second comparison electrode may be formed on a portion of the substrate where the micro-animal is placed on the substrate .

In an embodiment of the present invention, the plurality of second comparison electrodes may be provided on the substrate, and the plurality of second comparison electrodes may be spaced apart from each other.

According to an embodiment of the present invention, the apparatus may further include a partition wall portion extending upward from the substrate, wherein the partition wall portions are provided on the head side and the tail side of the micro-animal, respectively.

According to an embodiment of the present invention, the pair of barrier ribs are provided such that mutual spacing distances are adjustable, and the pair of barrier ribs are spaced apart from each other such that a distance corresponding to the length of the micro- And fixing the micro-animal.

According to an embodiment of the present invention, the apparatus may further include a circular wall extending upward from the substrate, wherein the circular wall is formed in a circular shape so as to surround the outer surface of the micro-animal.

In the embodiment of the present invention, the circular wall may be provided so as to be adjustable in diameter so as to correspond to the length of the micro-animal, thereby fixing the micro-animal having a different size.

According to an aspect of the present invention, there is provided a microfluidic device including: a substrate on which a micro-animal is placed; A fixer for accommodating the micro-animal in an inner space so that the micro-animal is fixed on the substrate; And a measuring unit for measuring a bio-signal of the micro-animal through state change information of the fixation fluid, wherein the micro-animal is fixed to the substrate by surface tension of the fixation liquid. Lt; / RTI >

In an embodiment of the present invention, the substrate may further include a third comparison electrode provided on one side of the substrate, and the third comparison electrode may be formed on a portion of the substrate where the micro-animal is placed on the substrate .

In an embodiment of the present invention, the measuring unit includes a working electrode, and the working electrode is inserted into the fixation solution accommodating the micro-animal, and the amount of current is measured through a potential difference with the third comparison electrode, And measuring a biological signal.

According to an embodiment of the present invention, the water tank may further include a water tank provided at an upper portion of the substrate to receive the fixer, wherein at least one micro animal is accommodated in the water tank.

In an embodiment of the present invention, it may further comprise a fourth comparison electrode extending upward from the substrate, the fourth comparison electrode being located inside the water receiving portion.

In an embodiment of the present invention, the measuring unit includes a working electrode, the working electrode being inserted into the fixation solution containing the micro-animal, measuring the amount of current through a potential difference with the fourth comparison electrode, And measuring a biological signal.

According to another aspect of the present invention, there is provided a method for measuring a biological signal of a micro-animal, comprising: fixing the micro-animal to the substrate; And measuring the biological signal by inserting the needle electrode into the micro animal, wherein in the step of fixing the micro animal to the substrate, the micro animal is fixed to the substrate by the surface tension of the fixation liquid And a measurement method of a biological signal measurement device for a micro animal.

In the embodiment of the present invention, in the step of measuring the bio-signal of the micro-animal, the needle electrode may be formed by a potential difference between the substrate formed by the first comparison electrode and the needle electrode, And measuring a potential difference between the needle electrode and the micro-animal to measure the bio-signal of the micro-animal.

In the embodiment of the present invention, in the step of fixing the micro-animal to the substrate, the micro-animal may be configured such that a distance between a pair of partition walls provided on the head side and the tail side of the micro- And is fixed and fixed so as to correspond to the first and second light sources.

In the embodiment of the present invention, in the step of fixing the micro-animal to the substrate, the micro-animal may have a diameter such that a circular wall formed in a circular shape to surround the outer surface of the micro-animal corresponds to the length of the micro- And is adjusted and fixed.

According to an aspect of the present invention, there is provided a method of measuring a biological animal signal measuring apparatus for a micro animal, the method comprising: fixing an animal to the substrate; And measuring a bio-signal of the micro-organ through the state change information of the fixation fluid measured by inserting a working electrode into the fixation fluid. In the step of fixing the micro-organ to the substrate, the micro- Wherein the immobilizing agent is immobilized on the substrate by the surface tension of the immobilizing liquid.

In the embodiment of the present invention, in the step of measuring the bio-signal of the micro-animal, the measurement unit may measure the potential difference between the fourth comparison electrode provided inside the water receiving unit and the working electrode inserted into the fixation solution accommodated in the water receiving unit And measuring the amount of current through the micro-organ to measure the bio-signal of the micro-animal.

The effect of the present invention with the above-described structure is that the micro-animal can be easily fixed and the biological signal of the micro-animal can be measured quickly.

In addition, since the fixative of the present invention is conductive, it can reflect the movement of muscles of the micro-animal accommodated and fixed within the fixative. Therefore, by inserting the working electrode into the fixative and measuring the amount of current, the electromyogram of the micro-animal can be inspected.

Further, in the present invention, in order to improve the electrical property of the fixative, the electrolyte solution may be further injected into the fixative to further improve the accuracy of the electromyographic test.

Further, according to the present invention, breathing of a micro-animal requiring a liquid for respiratory maintenance can be maintained for a long time.

In addition, according to the present invention, a drug can be injected into a fixative to easily perform a test on a micro animal.

Further, according to the present invention, inspection of a large amount of microalgae contained in a fixative can be easily performed.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

1 is an exemplary view of an apparatus for measuring a biological animal signal of a micro animal according to a first embodiment of the present invention.
FIG. 2 is a diagram illustrating an examination of a single micro-animal using an apparatus for measuring a bio-signal of a micro-animal according to a second embodiment of the present invention.
FIG. 3 is a diagram illustrating an examination of a plurality of micro-animals using an apparatus for measuring vital signs of micro-animals according to a second embodiment of the present invention.
4 is a view illustrating an apparatus for measuring a biological animal signal of a micro animal according to a third embodiment of the present invention.
FIG. 5 is a view illustrating an apparatus for measuring a biological animal signal of a micro-animal according to a fourth embodiment of the present invention.
FIG. 6 is a view illustrating an apparatus for measuring a biological animal signal of a micro-animal according to a fifth embodiment of the present invention.
FIG. 7 is a diagram illustrating an apparatus for measuring a biological animal signal of a micro animal according to a sixth embodiment of the present invention.
FIG. 8 is a flowchart illustrating a method of measuring an apparatus for measuring vital signs of a micro-animal according to the first to fourth embodiments of the present invention.
FIG. 9 is a flowchart illustrating a method of measuring an apparatus for measuring vital signs of micro-animals according to fifth and sixth embodiments of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and similar parts are denoted by like reference characters throughout the specification.

Throughout the specification, when a part is referred to as being "connected" (connected, connected, coupled) with another part, it is not only the case where it is "directly connected" "Is included. Also, when an element is referred to as "comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is an exemplary view of an apparatus for measuring a biological animal signal of a micro animal according to a first embodiment of the present invention.

As shown in FIG. 1, the biological animal signal measuring apparatus 10 for a micro animal according to the first embodiment may include a substrate 11, a fixing liquid 12, and a needle electrode 13.

The substrate 11 may be provided with a micro-animal placed thereon. Herein, the micro-animal Z may be a micro-animal to be an experimental subject, particularly a micro-animal Z having a gene similar to a human gene. For example, the micro animal Z may be a zebrafish, but the kind of the micro animal Z is not limited thereto. As an example, the fish may further include a fish.

Further, the substrate 11 according to the first embodiment may be characterized by being composed of a first comparison electrode.

The fixing liquid 12 may accommodate the micro-animal Z in the internal space so that the micro-animal Z is fixed on the substrate 11. Here, the fixing liquid 12 may include water, drugs, and the like.

The fixing liquid 12 may be placed on the substrate 11 together with the micro-organ Z through a transfer tool such as a pipette. The fixation liquid 12 placed on the substrate 11 while the micro-animal Z is housed therein can fix the micro-animal Z on the substrate 11 by surface tension . In this way, the micro-animal Z accommodated in the fixation liquid 12 can maintain breathing for a longer time than when it is in the air by the dissolved oxygen of the fixation liquid 12. Therefore, a more accurate test is possible.

The needle electrode 13 is inserted into the micro-animal Z accommodated in the fixation fluid 12 to measure the bio-signal of the micro-animal Z. The needle electrode 13 may include a working electrode.

When the needle electrode 13 including the working electrode is inserted into the micro animal Z, the bioelectric signal of the micro animal can be measured by measuring the potential difference between the needle electrode 13 and the first comparison electrode have.

The biological animal signal measuring apparatus 10 according to the first embodiment of the present invention may be configured to measure an EMG, an electrocardiogram, an electroencephalogram, or the like according to a position at which the needle electrode 13 is inserted into the micro- Can be inspected.

FIG. 2 is a diagram illustrating an examination of a single micro-animal using an apparatus for measuring a bio-signal of a micro-animal according to a second embodiment of the present invention.

2, the biological animal signal measuring apparatus 20 according to the second embodiment includes a substrate 21, a second comparing electrode 22, a fixing liquid 23, and a needle electrode 24 can do.

The substrate 21 may be provided with a micro-animal placed thereon. Herein, the micro-animal Z may be a micro-animal to be an experimental subject, particularly a micro-animal Z having a gene similar to a human gene. For example, the micro animal Z may be a zebrafish, but the kind of the micro animal Z is not limited thereto. As an example, the fish may further include a fish.

The second comparison electrode 22 may be provided on one surface of the substrate 21 and the second comparison electrode 22 may be formed on a portion of the substrate 21 on which the micro- . More specifically, the second comparative electrode 22 may be metal patterned on one surface of the substrate 21.

The fixation liquid 23 can accommodate the micro animal Z in the internal space so that the micro animal Z is fixed on the substrate 21. [ Here, the fixing liquid 23 may include water, drugs, and the like.

The fixation liquid 23 may be placed on the substrate 21 together with the micro-organ Z through a transporting tool such as a pipette. The fixation liquid 23 placed on the substrate 21 while the micro animal Z is housed therein can fix the micro animal Z on the substrate 21 by surface tension . In this way, the micro-animal Z accommodated in the fixation liquid 23 can maintain breathing for a longer time than when it is in the air by the dissolved oxygen of the fixation liquid 12. [ Therefore, a more accurate test is possible.

The needle electrode 24 may be inserted into the micro-animal Z accommodated in the fixation liquid 23 to measure the bio-signal of the micro-animal Z. The needle electrode 24 may include a working electrode.

The needle electrode 24 including the working electrode measures the potential difference between the needle electrode 24 and the second comparative electrode 22 when the needle electrode 24 is inserted into the micro animal Z, Can be measured.

The biological animal signal measuring apparatus 20 according to the second embodiment of the present invention may be configured to measure an EMG, an electrocardiogram, an electroencephalogram, or the like according to a position at which the needle electrode 24 is inserted into the micro- Can be inspected.

FIG. 3 is a diagram illustrating an examination of a plurality of micro-animals using an apparatus for measuring vital signs of micro-animals according to a second embodiment of the present invention.

3, in the biological animal signal measuring apparatus 20 according to the second embodiment, a plurality of the second comparing electrodes 22 are provided on the substrate 21, The two comparison electrodes 22 may be spaced apart from each other.

The apparatus 20 for measuring vital signs of micro-organisms according to the second embodiment of the present invention has a structure in which a large amount of micro-animals Z are fixed on a single substrate 21, and biological signals Can be measured. For example, the biological animal signal measuring apparatus 20 according to the second embodiment may be configured such that each micro-animal Z is received and fixed in a fixing liquid 23 containing different concentrations of drugs, Biological signals of the animal (Z) can be simultaneously measured and data can be obtained.

4 is a view illustrating an apparatus for measuring a biological animal signal of a micro animal according to a third embodiment of the present invention.

4, the biological animal signal measuring apparatus 30 according to the third embodiment includes a substrate 31, a second comparing electrode 32, a fixing liquid 33, a needle electrode 34, (35).

The substrate 31 may be provided so that a micro animal is placed thereon. Herein, the micro-animal Z may be a micro-animal to be an experimental subject, particularly a micro-animal Z having a gene similar to a human gene. For example, the micro animal Z may be a zebrafish, but the kind of the micro animal Z is not limited thereto. As an example, the fish may further include a fish.

The second comparison electrode 32 may be formed on one surface of the substrate 31 and the second comparison electrode 32 may be formed on a portion of the substrate 31 on which the micro- . More specifically, the second comparative electrode 32 may be metal patterned on one surface of the substrate 31.

The fixing liquid 33 can receive the micro-animal Z in the inner space so that the micro-animal Z is fixed on the substrate 31. [ Here, the fixer 33 may include water, drugs, and the like.

The fixing liquid 33 may be placed on the substrate 31 together with the micro-organ Z through a transfer tool such as a pipette. The fixation liquid 33 placed on the substrate 31 while the micro animal Z is housed therein can fix the micro animal Z on the substrate 31 by surface tension . In this way, the micro-animal Z accommodated in the fixation liquid 33 can maintain breathing for a longer time than when it is in the air by the dissolved oxygen of the fixation liquid 12. Therefore, a more accurate test is possible.

The needle electrode 34 may be inserted into the micro-animal Z accommodated in the fixing liquid 33 to measure the bio-signal of the micro-animal Z. The needle electrode 34 may include a working electrode.

When the needle electrode 34 including the working electrode is inserted into the micro animal Z, by measuring the potential difference between the needle electrode 34 and the second comparison electrode 32, Can be measured.

The partition wall portion 35 may extend from the substrate 31 to the upper portion and the partition wall portion 35 may be provided on the head side and the tail side of the micro animal Z, respectively.

The pair of partition walls 35 are arranged such that mutual spacing distances are adjustable and the pair of partition walls 35 are adjusted in spaced distance so as to correspond to the length of the micro- (Z) of the size of the micro-animal (Z).

The partition wall 35 may restrict the movement of the micro-animal Z so that the needle electrode 34 can be easily inserted into the micro-animal Z.

The bio-signal measuring device 30 according to the third embodiment of the present invention may be configured to measure an EMG, an electrocardiogram, an electroencephalogram, or the like according to a position at which the needle electrode 34 is inserted into the micro- Can be inspected.

FIG. 5 is a view illustrating an apparatus for measuring a biological animal signal of a micro-animal according to a fourth embodiment of the present invention.

5, the biological animal signal measuring apparatus 40 according to the fourth embodiment includes a substrate 41, a second comparing electrode 42, a fixing liquid 43, a needle electrode 44, May include a wall 45.

The substrate 41 may be provided so that a micro animal is placed thereon. Herein, the micro-animal Z may be a micro-animal to be an experimental subject, particularly a micro-animal Z having a gene similar to a human gene. For example, the micro animal Z may be a zebrafish, but the kind of the micro animal Z is not limited thereto. As an example, the fish may further include a fish.

The second comparison electrode 42 may be formed on one surface of the substrate 41 and the second comparison electrode 42 may be formed on a portion of the substrate 41 on which the micro- . More specifically, the second comparative electrode 42 may be metal patterned on one surface of the substrate 41.

The fixation fluid 43 may contain the micro-organism Z in the internal space so that the micro-organism Z is fixed on the substrate 41. Here, the fixing liquid 43 may include water, drugs, and the like.

The fixing liquid 43 may be placed on the substrate 41 together with the micro-organ Z through a transfer tool such as a pipette. The fixation liquid 43 placed on the substrate 41 while the micro animal Z is housed therein can fix the micro animal Z on the substrate 41 by surface tension . In this way, the micro-animal Z accommodated in the fixation liquid 43 can maintain breathing for a longer time than when it is in the air by the dissolved oxygen of the fixation liquid 12. [ Therefore, a more accurate test is possible.

The needle electrode 44 may be inserted into the micro-animal Z accommodated in the fixation fluid 43 to measure the bio-signal of the micro-animal Z. The needle electrode 44 may include a working electrode.

The needle electrode 44 including the working electrode measures the potential difference between the needle electrode 44 and the second comparison electrode 42 when the needle electrode 44 is inserted into the micro animal Z, Can be measured.

The circular wall 45 extends upward from the substrate 41 and the circular wall 45 may be formed in a circular shape so as to surround the outer surface of the micro animal Z. [ The circular wall 45 may be provided to adjust the diameter to correspond to the length of the micro-animal Z, and may fix the micro-animal Z having different sizes.

The circular wall 45 may be made of a material having flexibility to adjust the diameter.

The circular wall 45 thus provided can restrict the movement of the micro animal Z so that the needle electrode 44 can be easily inserted into the micro animal Z. [

The bio-signal measuring apparatus 40 according to the fourth embodiment of the present invention is provided with the micro-organism bio-signal measuring apparatus 40 according to the fourth embodiment of the present invention. Can be inspected.

FIG. 6 is a view illustrating an apparatus for measuring a biological animal signal of a micro-animal according to a fifth embodiment of the present invention.

6, the biological animal signal measuring apparatus 50 according to the fifth embodiment includes a substrate 51, a third comparing electrode 52, a fixing liquid 53, and a measuring unit (not shown) .

The substrate 51 may be provided with a micro-animal placed thereon. Herein, the micro-animal Z may be a micro-animal to be an experimental subject, particularly a micro-animal Z having a gene similar to a human gene. For example, the micro animal Z may be a zebrafish, but the kind of the micro animal Z is not limited thereto. As an example, the fish may further include a fish.

The third comparison electrode 52 may be formed on one surface of the substrate 51 and the third comparison electrode 52 may be formed on a portion of the substrate 51 on which the micro- . More specifically, the third comparison electrode 52 may be metal patterned on one surface of the substrate 51.

The fixing liquid 53 can accommodate the micro-animal Z in the internal space so that the micro-animal Z is fixed on the substrate 51. Here, the fixing liquid 53 may include water, drugs, and the like.

The fixing liquid 53 may be placed on the substrate 51 together with the micro-animal Z through a transfer tool such as a pipette. The fixation liquid 53 placed on the substrate 51 while the micro animal Z is housed therein can fix the micro animal Z on the substrate 51 by surface tension . In this way, the micro-animal Z accommodated in the fixation liquid 53 can maintain breathing for a longer time than when it is in the air due to the dissolved oxygen of the fixation liquid 12. Therefore, a more accurate test is possible.

The measurement unit may measure the bio-signal of the micro-animal through the change information of the fixation fluid 53. Specifically, since the fixation fluid 53 is made of a liquid having conductivity, the amount of current of the fixation fluid 53 can reflect the muscle movement of the micro-animal Z. Therefore, the measuring unit includes a working electrode. The working electrode is inserted into the fixing liquid 53 containing the micro-animal Z and measures the amount of current through a potential difference with the third comparing electrode 52, It is possible to measure the bio-signal of the micro-animal (Z).

The state change information may include at least one of a temperature of the fixation fluid 53 and a change in the surface tension of the fixation fluid 53 as well as a change in the flow of the fixation fluid 53 when the microorganism Z moves in the fixation fluid 53. [ And the like.

FIG. 7 is a diagram illustrating an apparatus for measuring a biological animal signal of a micro animal according to a sixth embodiment of the present invention.

7, the biological animal signal measuring apparatus 60 according to the sixth embodiment includes a substrate 61, a water receiving unit 62, a fixing liquid 63, a fourth comparison electrode 64, (Not shown).

The substrate 61 may be provided with one or more micro-animals placed thereon. Herein, the micro-animal Z may be a micro-animal to be an experimental subject, particularly a micro-animal Z having a gene similar to a human gene. For example, the micro animal Z may be a zebrafish, but the kind of the micro animal Z is not limited thereto. As an example, the fish may further include a fish.

The receiving portion 62 may be provided on the substrate 61 so as to receive the fixing liquid 63 on the inside thereof. One or more micro-animals may be accommodated inside the water receiving portion 62.

The fixing liquid 63 may receive the micro-animal Z in the internal space so that the micro-animal Z is fixed on the substrate 61. [ Here, the fixer 63 may include water, drugs, and the like.

The fixing liquid 63 may be placed on the substrate 61 together with the micro-organ Z through a transporting tool such as a pipette. The fixation liquid 63 placed on the substrate 61 while the micro animal Z is housed therein can fix the micro animal Z on the substrate 61 by surface tension . In this way, the micro-animal Z accommodated in the fixation liquid 63 can maintain breathing for a longer time than when it is in the air by the dissolved oxygen of the fixation liquid 12. [ Therefore, a more accurate test is possible.

The fourth comparison electrode 64 extends upward from the substrate 61 and may be located inside the water receiving portion 62. For example, as shown in FIG. 7, the fourth comparison electrode 64 may be provided in a columnar shape at an inner center portion of the water receiving portion 62, but is not limited thereto.

The measurement unit may measure the bio-signal of the micro-animal through the change information of the fixation fluid 63. [ Specifically, since the fixation fluid 63 is made of a liquid having conductivity, the amount of current of the fixation fluid 63 can reflect muscle movement of the micro-animal Z. Therefore, the measuring unit includes a working electrode, and the working electrode is inserted into the fixing liquid 63 in which the micro-animal Z is housed, and measures the amount of current through a potential difference with the fourth comparing electrode 64, It is possible to measure the bio-signal of the micro-animal (Z).

The state change information includes at least one of the temperature of the fixation fluid 63 and the surface tension of the fixation fluid 63 as well as the change of the surface tension of the fixation fluid 63 when the microorganism Z moves within the fixation fluid 63. [ And the like.

The apparatus 60 for measuring biological signals of micro-animals according to the sixth embodiment of the present invention can measure the bio-signals of the micro-animals Z housed inside the water receiving unit 62 on a group basis. For example, the biological animal signal measuring apparatus 60 according to the sixth embodiment may be configured such that each micro animal Z is separated into a group in which a dysthymic disorder model group and a muscle relaxant group are administered and placed in the water receiving unit 62 It is possible to measure the bio-signals per group by measuring the amount of current.

FIG. 8 is a flowchart illustrating a method of measuring an apparatus for measuring vital signs of a micro-animal according to the first to fourth embodiments of the present invention.

As shown in FIG. 8, the method for measuring a biological animal signal measuring apparatus according to any one of the first to fourth embodiments may further include the steps of: (S110) fixing the micro-animal to the substrate; And measuring a bio-signal by inserting the bio-signal (S120).

The micro-organism Z is fixed to the substrate 11, 21, 31, 41 by surface tension of the fixation liquid in the step of fixing the micro-animal to the substrate (S110).

In the method of measuring an apparatus for measuring a biological animal signal of a living animal according to the second embodiment, in the step (S110) of fixing the micro-animal to the substrate, the micro-animal (Z) The distance between the pair of partition walls 35 provided on the side of the micro animal Z is adjusted and fixed so as to correspond to the length of the micro animal Z. [

In the method of measuring an apparatus for measuring a biological animal signal of a small animal according to the third embodiment, in step S110 of fixing the micro animal to the substrate, the micro animal Z surrounds the outer surface of the micro animal Z And the diameter of the circular wall 45 is adjusted to correspond to the length of the micro animal Z. In this case,

The needle electrodes 13, 24, 34, and 44 are formed on the substrate 11 formed with the first reference electrode and the electrodes 11, 12, 13, 14, The potential difference of the needle electrodes 13 or the potential difference between the second comparison electrodes 22, 32, 42 formed on one surface of the substrate 21, 31, 41 and the needle electrodes 24, 34, And the bio-signal of the micro-animal (Z) is measured.

FIG. 9 is a flowchart illustrating a method of measuring an apparatus for measuring vital signs of micro-animals according to fifth and sixth embodiments of the present invention.

As shown in FIG. 9, the measuring method of a biological animal signal measuring apparatus according to the fifth and sixth embodiments includes the steps of fixing the micro-animal to the substrate (S210), inserting a working electrode into the fixed liquid And measuring the bio-signal of the micro-animal through the state change information of the fixation fluid (S220).

In the step S210 of fixing the micro-animal to the substrate, the micro-animal Z may be fixed to the substrate 51 or 61 by surface tension of the fixation liquid.

In the measuring method of a biological animal signal measuring apparatus according to the sixth embodiment, in step S220 of measuring the biological signal of the micro-organ through the state change information of the fixation liquid measured by inserting the working electrode into the fixation liquid, The measurement unit measures the amount of current through the potential difference between the fourth comparison electrode 64 provided inside the water receiving unit 62 and the working electrode inserted into the fixing liquid 63 received in the water receiving unit 62, And the bio-signal of the animal (Z) is measured.

The apparatus and method for measuring a biological animal signal of a micro animal as described above can easily fix the micro animal Z through surface tension of a water droplet fixation liquid and the micro animal Z can perform a breathing Can be maintained. That is, the micro-animal Z can be easily fixed and the respiration can be maintained, so that the biological signal of the micro-animal Z can be measured more quickly and accurately.

Further, according to the present invention, by changing the drug constituting the fixation liquid, it is possible to easily perform a test on the micro animal.

Further, according to the present invention, inspection of a large amount of microalgae contained in a fixative can be easily performed.

In particular, the present invention may further improve the accuracy of electromyography testing by further injecting an electrolyte into the fixative to improve the electrical properties of the fixative.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

10: Biological signal measurement device of micro-animal
11: substrate
12: fixative
13: Needle electrode
20: Bio-signal measurement device of micro-animal
21: substrate
22: second comparison electrode
23: fixative
24: Needle electrode
30: Apparatus for measuring biological signals of micro-animals
31: substrate
42: second comparison electrode
33: fixative
34: Needle electrode
35:
40: Biomedical signal measuring device for micro-animals
41: substrate
42: second comparison electrode
43: fixative
44: Needle electrode
45: Circular wall
50: Bio-signal measuring device of micro-animal
51: substrate
52: third comparison electrode
53: fixative
60: Apparatus for measuring biological signals of micro-animals
61: substrate
62:
63: fixative
64: fourth comparative electrode

Claims (20)

A substrate on which a micro animal is placed;
A fixer for accommodating the micro-animal in an inner space so that the micro-animal is fixed on the substrate; And
And a needle electrode inserted into the micro-animal accommodated in the fixer to measure a bio-signal of the micro-animal,
Wherein the micro-animal is fixed to the substrate by surface tension of the fixation liquid. The method according to claim 1,
Wherein the substrate comprises a first comparison electrode and measures a potential difference between the needle electrode and the first comparison electrode when the needle electrode is inserted into the micro animal to measure the biological signal of the micro animal. Apparatus for measuring biological signals of micro - animals. The method according to claim 1,
And a second comparison electrode provided on one surface of the substrate,
Wherein the second comparison electrode is formed on a portion of the micro-animal placed on the substrate. The method of claim 3,
Wherein the plurality of second comparison electrodes are provided on the substrate and the plurality of second comparison electrodes are spaced apart from each other. The method of claim 3,
Further comprising a partition wall portion extending upward from the substrate, wherein the partition wall portions are provided on the head side and the tail side of the micro-animal, respectively. 6. The method of claim 5,
The pair of barrier ribs,
Wherein the distance between the pair of partition walls is adjusted so that the distance between the pair of partition walls can be adjusted to correspond to the length of the micro-animal, thereby fixing the micro-animal having different sizes. Device. The method of claim 3,
Further comprising a circular wall extending upward from the substrate, wherein the circular wall is formed in a circular shape so as to surround the outer surface of the micro-animal. 8. The method of claim 7,
Wherein the circular wall is provided so as to be adjustable in diameter so as to correspond to the length of the micro-animal, thereby fixing the micro-animal having a different size. A substrate on which a micro animal is placed;
A fixer for accommodating the micro-animal in an inner space so that the micro-animal is fixed on the substrate; And
And a measurement unit for measuring a bio-signal of the micro-organ through the state change information of the fixation liquid,
Wherein the micro-animal is fixed to the substrate by surface tension of the fixation liquid. 10. The method of claim 9,
And a third comparison electrode provided on one surface of the substrate,
Wherein the third comparison electrode is formed in a part of the micro-animal placed on the substrate. 11. The method of claim 10,
Wherein the measuring portion includes a working electrode,
Wherein the working electrode is inserted into the fixation solution in which the micro-animal is housed, and measures the amount of current through a potential difference with the third comparison electrode to measure the bio-signal of the micro-animal. 10. The method of claim 9,
Further comprising a water tub provided at an upper portion of the substrate to receive the fixer,
Wherein at least one micro animal is accommodated in the water receiving part. 13. The method of claim 12,
Further comprising a fourth comparison electrode extending from the substrate toward the upper side and located inside the water receiving portion. 14. The method of claim 13,
Wherein the measuring portion includes a working electrode,
Wherein the working electrode is inserted into the fixation fluid in which the micro-animal is housed, and measures the amount of current through a potential difference with the fourth comparison electrode to measure the bio-signal of the micro-animal. 9. A method for measuring a biological animal signal of a living animal according to any one of claims 1 to 8,
Fixing the micro-animal to the substrate; And
And inserting the needle electrode into the micro animal to measure a living body signal,
Wherein in the step of fixing the micro-animal to the substrate, the micro-animal is fixed to the substrate by surface tension of the fixation liquid. 16. The method of claim 15,
In the step of measuring the bio-signal of the micro-animal,
The needle electrode measures the biological signal of the micro-animal by measuring a potential difference between the substrate formed of the first reference electrode and the needle electrode, or a potential difference between the second reference electrode formed on one surface of the substrate and the needle electrode And measuring the bio-signal of the micro-animal. 16. The method of claim 15,
In fixing the micro-animal to the substrate,
Wherein the micro animal is fixed and adjusted so that a distance between a pair of partition walls provided on the head side and the tail side of the micro animal corresponds to the length of the micro animal. Way. 16. The method of claim 15,
In fixing the micro-animal to the substrate,
Wherein the micro animal is fixed with a diameter controlled so that a circular wall formed in a circular shape to surround the outer surface of the micro animal corresponds to the length of the micro animal. The measuring method of an apparatus for measuring biological signals of micro-organisms according to any one of claims 9 to 14,
Fixing the micro-animal to the substrate; And
And measuring the bio-signal of the micro-organ through the state change information of the fixation liquid measured by inserting the working electrode into the fixation liquid,
Wherein in the step of fixing the micro-animal to the substrate, the micro-animal is fixed to the substrate by surface tension of the fixation liquid. 20. The method of claim 19,
In the step of measuring the bio-signal of the micro-animal,
Wherein the measurement unit measures the amount of electric current through a potential difference between a fourth comparison electrode provided inside the water receiving unit and the working electrode inserted into the fixation solution accommodated in the water receiving unit to measure the living body signal of the micro animal Measuring method of a living body signal measuring device of the present invention.

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