KR20180121031A - Method of manufacturing malt extract having low sweetness, and flavor enhanced non-alcohol beer-taste beverage comprising the malt extract - Google Patents
Method of manufacturing malt extract having low sweetness, and flavor enhanced non-alcohol beer-taste beverage comprising the malt extract Download PDFInfo
- Publication number
- KR20180121031A KR20180121031A KR1020170055181A KR20170055181A KR20180121031A KR 20180121031 A KR20180121031 A KR 20180121031A KR 1020170055181 A KR1020170055181 A KR 1020170055181A KR 20170055181 A KR20170055181 A KR 20170055181A KR 20180121031 A KR20180121031 A KR 20180121031A
- Authority
- KR
- South Korea
- Prior art keywords
- malt
- extract
- weight
- malt extract
- beverage
- Prior art date
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Seasonings (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
Description
본 발명은 저감미 맥아엑기스의 제조방법 및 이로 제조된 맥아엑기스를 포함하는 풍미가 향상된 무알코올 맥주맛 음료에 관한 것으로, 보다 상세하게는 기존 맥아엑기스보다 감미가 낮으며 활성탄 처리 및 정제 공정을 거친 맥아엑기스를 사용한 무알코올 맥주맛 음료를 제조함으로써 맥아엑기스의 사용량을 극대화할 수 있고, 무알코올 맥주맛 음료 제조 시 사용되는 엑기스분의 총량을 증가시키는 동시에 맥아 유래 잡미와 이취를 제거할 수 있기 때문에 맥주 특유의 풍부한 바디감을 가지면서 깔끔하고 풍미가 향상된 무알코올 맥주맛 음료에 관한 것이다.The present invention relates to a method for producing a reduced malt extract and a non-alcoholic beer flavored drink containing the malt extract prepared thereby. More particularly, the present invention relates to a method for producing a reduced malt malt extract which has lower sweetness than conventional malt extract, It is possible to maximize the use amount of malt extract by preparing a non-alcoholic beer flavored drink using malt extract, and to increase the total amount of the extract powder used in the production of non-alcoholic beer flavored beverage and to remove the malt- The present invention relates to a non-alcoholic beer-flavored beverage having a distinctive body feel characteristic of beer while improving the refreshing taste.
최근 음주운전사범 처벌 및 단속기준이 강화되고 음주운전에 대한 사회적 경각심이 강화되고 있으며, 음주로 인한 사회적 손실이 증가함에 따라 성인들이 흔히 즐기는 맥주와 유사한 맛과 향을 가지나 알코올이 없는 맥주맛 음료가 때와 장소에 대한 제약에 의해 음주를 할 수 없는 성인들에 각광을 받고 있다.Recently, drunken driving punishment and enforcement standards have been strengthened, social awareness about drunken driving has been strengthened, and as the social loss due to drinking increases, adults have similar taste and flavor to beer they enjoy, but alcohol-free beer taste drink It is attracting the attention of adults who can not drink because of restrictions on time and place.
이러한 무알코올 맥주맛 음료를 제조하는 방법으로는, 맥주와 동일한 방법으로 제조 공정에서 효모에 의한 알코올 발효를 진행 한 후 탈알코올 공정을 통해 알코올을 제거하여 효모에 의한 알코올 생성을 최소화하거나, 발효 공정 없이 배합을 통한 일반 음료 타입으로 제조하여 맥주와 유사한 맛과 향을 구현하는 방법이 알려져 있다.As a method for producing such a non-alcoholic beer flavored beverage, there is a method in which alcohol is fermented by a yeast in the manufacturing process in the same manner as beer, and then alcohol is removed through a de-alcohol process to minimize the production of alcohol by yeast, It is known that a beverage is produced in a general beverage type through mixing to realize a flavor and aroma similar to beer.
상기 무알코올 맥주맛 음료의 제조 방법 중에서 탈알코올 공정을 거치는 제조법은 알코올을 발생시켜 이후 여과 또는 역삼투를 통해 알코올을 제거하므로 알코올이 일부 잔존할 가능성이 존재하는 반면 맥주와 같거나 유사한 원재료를 사용하여 발효공정을 거치지 않은 비발효 무알코올 맥주맛 음료의 제조법은 원료단계부터 알코올의 혼입을 완전 차단하여 알코올의 잔존 가능성을 제거해 특정한 조건에서 술을 섭취할 수 없는 직장인이나, 임산부 등 소비자층에서도 음용이 가능하며, 제조에 오랜 시간이 소요되지 않으며, 단순한 제조 공정으로 맥주와 유사한 풍미를 구현 할 수 있는 장점이 있어 최근 각광받고 있는 제조 방법이다.Among the processes for producing non-alcoholic beer flavored beverages, the alcohol alcohol-producing process removes alcohol through filtration or reverse osmosis, so that there is a possibility that a part of alcohol is left, while a similar or similar raw material is used The non-fermented non-alcoholic beer flavored beverage which has not undergone the fermentation process completely blocks the incorporation of alcohol from the raw material stage, thereby eliminating the possibility of residual alcohol, so that the drink can not be consumed under certain conditions, It is possible to produce a beer-like flavor in a simple manufacturing process because it does not take a long time to manufacture, and it is a manufacturing method that has recently become popular.
한편, 알코올이 없으며, 맥주와 유사한 관능적 특성을 개선하고 품질 안정성을 확보하기 위한 기술이 활발하게 연구되고 있다.On the other hand, there is no alcohol, and techniques for improving sensory characteristics similar to beer and securing quality stability are actively researched.
대한민국공개특허 2014-0104970 에는 단당류 및 이당류의 비율이 높은 논알코올의 맥주 테이스트 음료를 제조하는 방법이 기재되어 있다. 이러한 논알코올의 맥주 테이스트 음료를 제조하는 방법은 엑기스분의 총량이 0.1 ~ 0.5 중량%이고, 삼당류의 중량에 대한 단당류 및 이당류의 중량의 합계의 비(단당류 및 이당류의 w/v%의 합계/삼당류의 w/v%)가 2.5 ~ 11인 논알코올의 맥주 테이스트 음료를 제조하는 것을 특징으로 한다. Korean Patent Laid-Open Publication No. 2014-0104970 discloses a method for producing a non-alcoholic beer taste drink having a high proportion of monosaccharides and disaccharides. The method for producing the non-alcoholic beer tasted beverage is characterized in that the total amount of the extract powder is 0.1 to 0.5 wt% and the ratio of the total weight of the monosaccharide and the disaccharide to the weight of the trisaccharide (the sum of w / v% / W / v% of trisaccharides) of 2.5 to 11 is produced from non-alcoholic beverages.
그러나, 상기 제조방법은 저당질과 저칼로리를 실현하기 위해 전체 엑기스분의 총량을 작게 하기 위해 당질 중 바디감을 느끼게 하는 삼당류 이상 탄수화물의 비율을 낮추어 제조하여, 뒷맛의 깔끔함이 부여될 수는 있을지 몰라도 최근 트렌드인 바디감이 풍부한 맥주와 유사한 맛을 구현하기는 어려운 문제가 있었다.However, in order to realize a low sugar content and a low calorie content, the above-described preparation method may be carried out by lowering the proportion of trisaccharide or higher carbohydrate that makes the body feel a sense of body fat in order to reduce the total amount of whole extract, There is a problem that it is difficult to realize a flavor similar to a beer rich in body sensation in recent trends.
본 발명은 기존 맥아엑기스보다 단당류와 이당류의 함량이 낮아 감미가 낮으며, 활성탄 처리 및 정제공정을 거쳐 잡미와 이취를 제거한 맥아엑기스를 제조하는 방법, 및 이러한 맥아엑기스를 포함하는 맥주 특유의 풍부한 바디감을 가지면서 깔끔하고 풍미가 향상된 무알코올 음료를 제공하는 것이다.The present invention relates to a method for producing a malt extract which has lower sweetness and disaccharide content than conventional malt extract and has a low sweetness and which is free from odor and odor through an activated carbon treatment and purification process and a method for producing a malt extract- To provide a refreshing, non-alcoholic beverage with improved flavor.
본 발명의 일 측면에 따른 저감미 맥아엑기스의 제조방법은, 맥아를 셀룰라아제(cellulase)로 1차 효소처리하는 단계; 상기 1차 효소처리한 맥아를 α-아밀라아제(amylase)로 2차 효소처리 하는 단계; 및 상기 2차 효소처리한 맥아를 활성탄 처리, 정제, 및 농축하는 단계를 포함한다.According to an aspect of the present invention, there is provided a method for producing a reduced malt extract, comprising the steps of: treating a malt with a cellulase; Treating the primary enzyme-treated malt with a secondary enzyme with? -Amylase; And treating the malt treated with the secondary enzyme with activated carbon, refining, and concentrating.
그리고, 상기 1차 효소처리는 40 내지 85 ℃에서 1 내지 5시간 동안 처리하고, 상기 2차 효소처리는 45 내지 95 ℃에서 1 내지 7시간 동안 처리할 수 있다.The primary enzyme treatment may be performed at 40 to 85 ° C for 1 to 5 hours, and the secondary enzyme treatment may be treated at 45 to 95 ° C for 1 to 7 hours.
또한, 상기 셀룰라아제는 상기 맥아 100중량부에 대하여 0.1 내지 10 중량부로 첨가될 수 있다.The cellulase may be added in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of the malt.
그리고, 상기 α-아밀라아제는 상기 맥아 100중량부에 대하여 0.1 내지 10 중량부로 첨가될 수 있다.The α-amylase may be added in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of the malt.
한편, 상기 활성탄 처리는 활성탄을 상기 맥아 중량 대비 100 내지 10,000 ppm 농도로 0.5 내지 3시간 동안 상온에서 처리할 수 있다.On the other hand, in the activated carbon treatment, the activated carbon may be treated at a concentration of 100 to 10,000 ppm based on the weight of the malt for 0.5 to 3 hours at room temperature.
본 발명의 다른 측면에 따른 저감미 맥아엑기스는 전체 맥아엑기스(20°Bx 기준) 중 단당류 및 이당류가 3 내지 15중량%로 포함된다.The reduced malt extract according to another aspect of the present invention contains 3 to 15% by weight of monosaccharide and disaccharide in the total malt extract (20 ° Bx standard).
본 발명의 또 다른 측면에 따른 무알코올 맥주맛 음료는 상기 저감미 맥아엑기스를 포함하는 엑기스분을 포함한다.A non-alcoholic beer flavored beverage according to another aspect of the present invention includes an extract powder containing the reduced malt extract.
그리고, 상기 맥아엑기스는 무알코올 맥주맛 음료 중 0.03 내지 15 중량%로 포함될 수 있다.The malt extract may be contained in an amount of 0.03 to 15% by weight of the non-alcoholic beer flavored beverage.
또한, 상기 무알코올 맥주맛 음료 중 단당류 및 이당류의 함량은 0.5g/100ml 이하일 수 있다.In addition, the content of the monosaccharide and the disaccharide in the non-alcoholic beer flavored beverage may be 0.5 g / 100 ml or less.
한편, 상기 엑기스분은 무알코올 맥주맛 음료 중 2 내지 4 중량%로 포함될 수 있다.On the other hand, the extract may be contained in an amount of 2 to 4% by weight of the non-alcoholic beer flavored beverage.
그리고, 상기 엑기스분은 덱스트린, 거품 생성제, 대두다당류, 및 쓴맛 물질을 포함할 수 있다.And, the extract powder may contain dextrin, a foaming agent, a soy polysaccharide, and a bitter taste material.
또한, 상기 덱스트린은 난소화성말토덱스트린, 말토덱스트린, 및 폴리덱스트로오스로 이루어지는 군으로부터 선택되는 하나 이상일 수 있고, 상기 덱스트린은 전체 엑기스분에서 10 내지 85중량%로 포함될 수 있다.The dextrin may be at least one selected from the group consisting of indominant maltodextrin, maltodextrin, and polydextrose, and the dextrin may be contained in an amount of 10 to 85% by weight of the whole extract.
한편, 상기 거품 생성제는 두류 단백 분해물일 수 있고, 상기 두류 단백 분해물은 대두 펩타이드 또는 완두 펩타이드 일 수 있다.On the other hand, the foam-forming agent may be a soybean protein degradation product, and the soybean protein degradation product may be a soybean peptide or pea peptide.
또한, 상기 거품 생성제는 전체 엑기스분에서 0.1 내지 20중량%로 포함될 수 있고, 상기 대두 다당류는 전체 엑기스분에서 0.1 내지 20중량%로 포함될 수 있다.In addition, the foaming agent may be contained in an amount of 0.1 to 20% by weight in the whole extract, and the soy polysaccharide may be contained in an amount of 0.1 to 20% by weight in the whole extract.
그리고, 상기 쓴맛 물질은 iso-αacid일 수 있고, 상기 iso-αacid 의 농도가 0.0001g/L 이상일 수 있다.The bitter taste material may be iso-αacid, and the concentration of iso-αacid may be 0.0001 g / L or more.
본 발명의 또 다른 측면에 따른 무알코올 맥주맛 음료는 NIBEM이 50 이상일 수 있고, 당류의 함량이 0.01 내지 0.5중량%이고, 100ml 당 칼로리가 0.1 내지 20 kcal 일 수 있으며, 비발효인 무알코올 맥주맛 음료일 수 있다.According to another aspect of the present invention, a non-alcoholic beer flavored drink may have a NIBEM of 50 or more, a saccharide content of 0.01 to 0.5% by weight, a calorie of 100 ml to 0.1 to 20 kcal, and a non-fermented non- It can be a flavored drink.
본 발명에 따른 저감미 맥아엑기스의 제조방법에 의하면, 단당류와 이당류의 함량이 낮아 기존 맥아엑기스보다 감미가 낮으며, 활성탄 처리 및 정제과정을 거쳐 잡미와 이취가 제거된 맥아엑기스를 제조할 수 있다.According to the method for producing the reduced malt extract according to the present invention, the malt extract can be prepared by removing the odor and odor from the malt extract by lowering the sweetness and disaccharide content, .
또한, 이러한 맥아엑기스를 포함하여 무알코올 맥주맛 음료를 제조 시 맥아엑기스의 사용량을 극대화할 수 있고, 무알코올 맥주맛 음료 제조 시 사용되는 엑기스분의 총량을 증가시킬 수 있기 때문에 맥주 특유의 풍부한 바디감을 가지면서 잡미와 이취가 제거되어 깔끔하고 풍미가 향상된 무알코올 맥주맛 음료를 제조할 수 있다.In addition, it is possible to maximize the amount of malt extract used in the production of non-alcoholic beer flavored beverages including malt extract, and to increase the total amount of extract fractions used in the production of non-alcoholic beer flavored beverage, And it is possible to produce a non-alcoholic beer flavored drink which is clean and has improved flavor by removing the odor and odor.
도 1은, 본 발명의 일 실시예에 따른 저감미 맥아엑기스의 제조방법을 나타낸 순서도이다.
도 2는, 본 발명의 일 실시예에 따른 저감미 맥아엑기스를 포함하는 무알코올 맥주맛 음료의 제조방법을 나타낸 순서도이다.1 is a flowchart showing a method for manufacturing a reduced malt extract according to an embodiment of the present invention.
FIG. 2 is a flowchart illustrating a method for producing a non-alcoholic beer flavored beverage including a reduced malt extract according to an embodiment of the present invention.
본 발명은 다양한 변환을 가할 수 있고 여러 가지 실시예를 가질 수 있는 바, 특정 실시예들을 도면에 예시하고 상세한 설명에 상세하게 설명하고자 한다. 그러나, 이는 본 발명을 특정한 실시 형태에 대해 한정하려는 것이 아니며, 본 발명의 사상 및 기술 범위에 포함되는 모든 변환, 균등물 내지 대체물을 포함하는 것으로 이해되어야 한다. 본 발명을 설명함에 있어서 관련된 공지 기술에 대한 구체적인 설명이 본 발명의 요지를 흐릴 수 있다고 판단되는 경우 그 상세한 설명을 생략한다.BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and described in detail in the detailed description. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.
본 발명은, 맥아를 셀룰라아제(cellulase)로 1차 효소처리하는 단계; 상기 1차 효소처리한 맥아를 α-아밀라아제(amylase)로 2차 효소처리 하는 단계; 및 상기 2차 효소처리한 맥아를 활성탄 처리, 정제, 및 농축하는 단계를 포함하는 저감미 맥아엑기스의 제조방법을 제공한다.The present invention relates to a method for producing maltose, comprising the steps of: treating a malt with a cellulase as a primary enzyme; Treating the primary enzyme-treated malt with a secondary enzyme with? -Amylase; And a step of treating, purifying, and concentrating the malt treated with the secondary enzyme with activated charcoal, thereby obtaining a reduced malt extract.
또한, 본 발명의 다른 측면에 따르면 전체 맥아엑기스(20°Bx 기준) 중 단당류 및 이당류가 3 내지 15중량%로 포함되는 저감미 맥아엑기스가 제공된다.According to another aspect of the present invention, there is provided a reduced malt extract having 3 to 15% by weight of monosaccharide and disaccharide in whole malt extract (20 ° Bx standard).
또한, 본 발명의 또 다른 측면에 따르면 상기 저감미 맥아엑기스를 포함하는 엑기스분을 포함하는 풍미가 향상된 무알코올 맥주맛 음료가 제공된다.According to another aspect of the present invention, there is provided a non-alcoholic beer-flavored beverage having improved flavor comprising the extract powder containing the reduced malt extract.
이하 발명의 구체적인 구현예에 따른 저감미 맥아엑기스의 제조방법 및 이를 포함하는 풍미가 향상된 무알코올 맥주맛 음료에 관하여 보다 상세하게 설명하기로 한다.Hereinafter, a method for producing the reduced malt extract and a non-alcoholic beer flavored drink having improved flavor according to a specific embodiment of the present invention will be described in detail.
종래 맥아엑기스는 감미가 높아 무알코올 맥주맛 음료에 사용 시 사용량에 제한이 있다는 문제가 있었고, 저당질과 저칼로리를 실현하기 위해 전체 엑기스분의 총량을 작게 하기 위해 당질 중 바디감을 느끼게 하는 삼당류 이상의 탄수화물의 비율을 낮추어 제조하는 경우, 바디감이 풍부한 맥주와 유사한 맛을 구현하기는 어려운 문제점이 있었다.Conventionally, malt extract has a high sweetness, so there is a problem that there is a limitation in the amount of the malt extract used when used in non-alcoholic beer flavored beverages. In order to realize low sugar content and low calorie content, malt extract There is a problem that it is difficult to realize a taste similar to a beer rich in body sensation when the carbohydrate ratio is lowered.
이에 본 발명자들은 특정 효소들로 1차, 2차 효소 처리를 행하는 경우 감미가 낮은 맥아엑기스를 제조할 수 있어 이러한 저감미 맥아엑기스를 사용하여 무알코올 맥주맛음료를 제조하는 경우, 맥주 특유의 드라이하며 풍부한 바디감을 가지면서 깔끔하고 풍미가 향상된 무알코올 맥주맛 음료를 제조할 수 있다는 점을 실험을 통하여 확인하고 발명을 완성하였다.Therefore, the inventors of the present invention can produce a malt extract having low sweetness when the first and second enzymes are treated with specific enzymes. In the case of producing a non-alcoholic beer flavored drink using such reduced malt extract, It was confirmed through experiments that the beverage can be made in a non-alcoholic beer with a rich body and a clean and improved flavor.
이하, 본 발명에 따른 저감미 맥아엑기스의 제조방법에 대해 상술한다.Hereinafter, a method for producing the reduced-malt extract according to the present invention will be described in detail.
본 발명의 일 측면에 따른 저감미 맥아엑기스의 제조방법은, 맥아를 셀룰라아제(cellulase)로 1차 효소처리하는 단계; 상기 1차 효소처리한 맥아를 α-아밀라아제(amylase)로 2차 효소처리 하는 단계; 및 상기 2차 효소처리한 맥아를 활성탄 처리, 정제, 및 농축하는 단계를 포함한다.According to an aspect of the present invention, there is provided a method for producing a reduced malt extract, comprising the steps of: treating a malt with a cellulase; Treating the primary enzyme-treated malt with a secondary enzyme with? -Amylase; And treating the malt treated with the secondary enzyme with activated carbon, refining, and concentrating.
도 1은, 본 발명의 일 실시예에 따른 저감미 맥아엑기스의 제조방법을 나타낸 순서도이다. 도 1에서 나타난 바와 같이 셀룰라아제로 1차 효소 처리 및 α-아밀라아제로 2차 효소 처리를 행한다는 점과, 활성탄 처리를 통해 감미와 이취가 제거된다는 점, 및 정밀 5차 여과 공정을 거친다는 점을 특징으로 하고 있다.1 is a flowchart showing a method for manufacturing a reduced malt extract according to an embodiment of the present invention. As shown in Fig. 1, the cellulase is subjected to the first enzyme treatment and the second enzyme treatment with? -Amylase, the sweetener and the odor are removed through the activated carbon treatment, and the fine fifth- .
상기에서 맥아란 발아한 보리를 건조시키고, 뿌리를 제거한 것을 의미한다.In the above, malt means that germinated barley is dried and the roots are removed.
상기 셀룰라아제는 섬유소분해효소로 Celluclast, Viscozyme, Ceramix, 및 Ultraflo 에서 선택되는 하나 이상을 사용할 수 있다.The cellulase may be one or more selected from Cellulase, Viscozyme, Ceramix, and Ultraflo as a cellulolytic enzyme.
상기 셀룰라아제는 상기 맥아 100중량부에 대하여 0.1 내지 10 중량부로 첨가될 수 있다.The cellulase may be added in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of the malt.
또한, 상기 α-아밀라아제는 액화효소로 Termamyl, BAN, 및 Fungamyl 에서 선택되는 하나 이상을 사용할 수 있다.Further, the? -Amylase can be one or more selected from Termamyl, BAN, and Fungamyl as liquefying enzymes.
상기 α-아밀라아제는 상기 맥아 100중량부에 대하여 0.1 내지 10 중량부로 첨가될 수 있다.The α-amylase may be added in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of the malt.
한편, 상기 1차 효소처리에서 셀룰라아제는 식물세포벽분해 복합효소로써 식물유래 원료에 존재하는 불용성의 고분자물질 등을 가용화하여 추출율을 향상시키는 역할을 하고, 2차 효소처리에서 α-아밀라아제는 글루코오스의 α-1,4 결합에 작용하여 글루코오스 사슬을 점차 분해하는 역할을 한다. 이러한 셀룰라아제 및 α-아밀라아제 등의 효소 처리를 통한 맥아엑기스 제조방법은 종래 맥아에서 유래한 맥아 자체 효소에 의해 제조된 맥아엑기스와 비교하여 액화 및 당화를 통한 당류의 함량을 조절할 수 있으며, 특히 셀룰라아제와 α-아밀라아제 효소를 순차적인 처리를 통해 효과적인 탄수화물의 분해가 가능해져 각각의 효소 마다 최적 반응 조건을 설정하여 결국 저감미 맥아엑기스를 제조할 수 있다.On the other hand, in the first enzyme treatment, the cellulase is a plant cell wall degradation complex enzyme, which solubilizes the insoluble polymer substances present in the plant-derived raw material to improve the extraction ratio. In the secondary enzyme treatment, the? -Amylase is? -1,4 bond and gradually decompose the glucose chain. The method for producing malt extract through enzyme treatment such as cellulase and? -Amylase can control the content of saccharide by liquefaction and saccharification compared with malt extract prepared by the malt enzyme derived from malt in the past. Especially, The α-amylase enzyme is capable of degrading the carbohydrate effectively through sequential treatment, and the optimal reaction conditions are set for each enzyme, thereby producing the reduced malt extract.
상기 1차 효소처리는 40 내지 85 ℃에서 1 내지 5시간 동안 처리되는 것이 바람직할 수 있고, 2차 효소처리는 45 내지 95 ℃에서 1 내지 7시간 동안 처리되는 것이 바람직할 수 있다.The primary enzyme treatment may be preferably treated at 40 to 85 ° C for 1 to 5 hours, and the secondary enzyme treatment may be preferably treated at 45 to 95 ° C for 1 to 7 hours.
한편, 상기 활성탄 처리는 활성탄을 상기 맥아 중량 대비 100 내지 10,000 ppm 농도로 0.5 내지 3시간 동안 상온에서 처리할 수 있다.On the other hand, in the activated carbon treatment, the activated carbon may be treated at a concentration of 100 to 10,000 ppm based on the weight of the malt for 0.5 to 3 hours at room temperature.
상기와 같은 방법으로 제조되는 맥아엑기스는 전체 맥아엑기스(20°Bx 기준) 중 단당류 및 이당류가 5 내지 15중량%로 포함된 저감미 맥아엑기스 일 수 있다.The malt extract prepared as described above may be a reduced malt extract containing 5 to 15% by weight of monosaccharide and disaccharide in whole malt extract (20 ° Bx standard).
한편, 본 명세서에서의 당류란, 단당류, 이당류를 의미하며 삼당류 이상의 올리고당을 제외한 탄수화물을 의미할 수 있다. 단당류로는 글루코오스, 프룩토오스, 갈락토오스, 만노오스 등을 들 수 있고, 이당류로는 수크로오스, 유당, 맥아당, 트레할로오스, 셀로비오스 등을 들 수 있다. 삼당류 이상의 올리고당으로는, 스타키오스, 말토트리오스, 말토테트라오스, 말토펜타오스 등을 들 수 있다.On the other hand, in the present specification, sugars means monosaccharides and disaccharides, and can mean carbohydrates excluding oligosaccharides of trisaccharides or more. Examples of the monosaccharide include glucose, fructose, galactose and mannose. Examples of the disaccharide include sucrose, lactose, maltose, trehalose, and cellobiose. Examples of oligosaccharides having three or more trisaccharides include starch, maltotriose, maltotetraose and maltopentaose.
이러한 당류의 양은 음료 전체의 중량으로부터 단백질, 지질, 식물섬유, 회분, 알코올분, 및 수분의 양을 빼는 것에 의해 산출할 수 있다.The amount of this saccharide can be calculated by subtracting the amount of proteins, lipids, plant fibers, ash, alcohol, and water from the weight of the whole beverage.
상술한 저감미 맥아엑기스의 제조방법에 의하면, 잡미와 이취가 제거되고, 단당류와 이당류의 함량이 낮아 기존 맥아엑기스보다 감미가 낮고, 활성탄 처리 및 정제 공정을 통해 맥즙 특유의 잡미와 이취를 제거한 맥아엑기스를 제조할 수 있다.According to the method for producing the reduced malt extract, maltose and odor are removed, the content of the monosaccharide and the disaccharide is low and the sweetness is lower than that of the existing malt extract, and malt and malt The extract can be produced.
이하에서는 상술한 저감미 맥아엑기스를 포함하는 엑기스분을 포함하는 풍미가 향상된 무알코올 맥주맛 음료에 대하여 상세히 설명한다.Hereinafter, the non-alcoholic beer flavored beverage having improved flavor including the extract powder containing the above-described reduced malt extract will be described in detail.
도 2는, 본 발명의 일 실시예에 따른 저감미 맥아엑기스를 포함하는 무알코올 맥주맛 음료의 제조방법을 나타낸 순서도이다.FIG. 2 is a flowchart illustrating a method for producing a non-alcoholic beer flavored beverage including a reduced malt extract according to an embodiment of the present invention.
본 명세서에서 무알코올 맥주맛 음료란, 알코올 함량이 1% 미만인 맥주와 같은 풍미를 갖는 탄산 음료를 말하며, 효모에 의한 발효 공정의 유무에 관계 없이 맥주 풍미의 음료를 모두 포함할 수 있다.In the present specification, the non-alcoholic beer flavored drink refers to a carbonated drink having a flavor such as a beer having an alcohol content of less than 1%, and may include beer flavored beverages regardless of whether or not the fermentation process is performed by yeast.
또한, 본 발명은 비발효 무알코올 음료로 이것은 알코올을 1% 미만으로 함유하여 실질적으로 함유하지 않는 것으로 취급될 수 있다. 즉, 본 발명의 무알코올 맥주맛 음료는 검출할 수 없을 정도의 극미량의 알코올을 함유하는 음료를 포함할 수 있다.Further, the present invention can be regarded as a non-fermentation non-alcoholic beverage which contains less than 1% alcohol and is substantially free from alcohol. That is, the non-alcoholic beer-flavored beverage of the present invention may contain a beverage containing a trace amount of alcohol that can not be detected.
한편, 비발효란 미생물에 의한 유기물의 분해가 일어나지 않는 것을 말하고, 특히 효모에 의한 유기물의 분해에 의해 알코올이 생성되지 않는 것을 의미할 수 있다.On the other hand, non-fermentation means that organic matter is not decomposed by microorganisms, and in particular, alcohol is not produced by decomposition of organic matter by yeast.
무알코올 맥주맛 음료에 포함되는 저감미 맥아엑기스를 포함하는 엑기스분은 무알코올 맥주맛 음료의 주원료를 의미한다.Non-alcoholic beverages The extracts containing reduced malt extract contained in beverages mean the main ingredient of non-alcoholic beer-flavored beverages.
상술한 저감미 맥아엑기스는 감미가 낮으며, 맥즙 특유의 이취와 잡미를 제거하여 실제 음료 제품 중 첨가량을 극대화할 수 있고, 결국 무알코올 맥주맛 음료 제조 시 사용되는 엑기스분의 총량을 증가시켜 바디감을 향상 시킬 수 있다.The above-described low-malt malt extract has a low sweetness, and it is possible to maximize the addition amount of the actual beverage products by removing the odor and odor peculiar to the wort, thereby increasing the total amount of the extract fractions used in the non- Can be improved.
본 발명의 일 측면에 따르면, 맥아엑기스는 무알코올 맥주맛 음료 중 0.03 내지 15 중량%(20°Bx 기준) 로 포함될 수 있다. 또한, 총 엑기스분은 무알코올 맥주맛 음료 중 2 내지 4 중량%로 포함될 수 있다.According to one aspect of the present invention, the malt extract may be included in 0.03 to 15 wt% (based on 20 Bx) of the non-alcoholic beer flavored beverage. In addition, the total extract powder may be contained in an amount of 2 to 4% by weight in the non-alcoholic beer flavored drink.
한편, 무알코올 맥주맛 음료 제조 시 상술한 저감미 맥아엑기스를 사용하였기 때문에 무알코올 맥주맛 음료 중 단당류 및 이당류의 함량은 0.5g/100ml 이하로 포함될 수 있다.On the other hand, since the above-described low-sensitive malt extract is used in the production of non-alcoholic beer flavored drinks, the content of monosaccharides and disaccharides in non-alcoholic beer flavored beverages can be less than 0.5 g / 100 ml.
상기 엑기스분은 덱스트린, 거품 생성제, 대두다당류, 및 쓴맛 물질 등 일반적인 무알코올 맥주맛 음료 제조 시 사용될 수 있는 엑기스분을 포함한다.The extract powder includes an extract powder which can be used in the production of a general non-alcoholic beverage flavored drink such as dextrin, a foaming agent, a soy polysaccharide, and a bitter taste material.
상기 덱스트린은 일반적으로 무알코올 맥주맛 음료 제조 시 사용되는 것이라면 특별히 제한되지는 않으나, 난소화성말토덱스트린, 말토덱스트린, 및 폴리덱스트로오스로 이루어지는 군으로부터 선택되는 하나 이상일 수 있다.The dextrin may be one or more selected from the group consisting of indominant maltodextrin, maltodextrin, and polydextrose, although it is not particularly limited as long as it is generally used in the production of non-alcoholic beer flavored beverages.
그리고, 상기 덱스트린은 전체 엑기스분에서 10 내지 85중량%로 포함될 수 있다.The dextrin may be contained in an amount of 10 to 85% by weight in the whole extract.
또한, 상기 거품 생성제는 두류 단백 분해물일 수 있고, 상기 두류 단백 분해물은 대두 펩타이드 또는 완두 펩타이드일 수 있다. In addition, the foaming agent may be a soybean protein degradation product, and the soybean protein degradation product may be a soybean peptide or pea peptide.
그리고, 상기 거품 생성제는 전체 엑기스분에서 0.1 내지 20중량%로 포함될 수 있다.In addition, the foaming agent may be contained in an amount of 0.1 to 20% by weight in the whole extract.
한편, 상기 대두 다당류는 일반적으로 무알코올 맥주맛 음료 제조 시 사용되는 것이라면 특별히 제한되지 아니하고, 함량은 전체 엑기스분에서 0.1 내지 20중량%로 포함될 수 있다.On the other hand, the soy polysaccharide is not particularly limited as long as it is generally used in the production of non-alcoholic beer flavored beverages, and the content thereof may be 0.1 to 20% by weight in the whole extract.
그리고, 상기 쓴맛 물질로 호프 내에 포함된 iso-αacid 를 사용할 수 있으며, iso-αacid 의 농도가 0.0001g/L 이상인 것이 바람직할 수 있다.The iso-αacid contained in the hop may be used as the bitter taste material, and it may be preferable that the concentration of iso-αacid is 0.0001 g / L or more.
한편, 본 발명의 일 측면에 따른 무알코올 맥주맛 음료는 거품 유지 시간을 측정한 값인 NIBEM이 50 이상일 수 있다.Meanwhile, the non-alcoholic beverage flavored beverage according to one aspect of the present invention may have a NIBEM value of 50 or more, which is a value obtained by measuring the bubble retention time.
그리고, 본 발명의 일 측면에 따른 무알코올 맥주맛 음료는 당류의 함량이 0.01 내지 0.5중량%이고, 100ml 당 칼로리가 0.1 내지 20 kcal 일 수 있다.The non-alcoholic beer-flavored beverage according to one aspect of the present invention may have a saccharide content of 0.01 to 0.5% by weight, and a calorie per 100 ml may be 0.1 to 20 kcal.
한편, 본 발명에 있어 본 발명의 효과를 방해하지 않은 한 식품 첨가물로서 인정받고 있는 성분을 제한 없이 사용 가능하다.On the other hand, components which are recognized as food additives in the present invention can be used without limitation without interfering with the effect of the present invention.
이하, 본 발명의 바람직한 실시예를 첨부도면을 참조하여 상세히 설명하기로 한다. 다만, 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는다 할 것이다.Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. It should be understood, however, that these examples are for illustrative purposes only and are not to be construed as limiting the scope of the present invention.
실시예Example 1 : One : 저감미Reduced 맥아엑기스Malt extract 제조 Produce
맥아 1kg을 정제수 2배수에 침지 후 분쇄한 것을 추출탱크에 셀룰라아제 2g(0.2%)와 함께 투입을 하여 60℃에서 2시간 동안 1차 효소처리를 하였다. 1차 효소처리 후 α-아밀라아제 20g(2%)를 추가 투입하여 85℃로 승온하여 3시간 동안 2차 효소처리를 하였다. 효소처리가 끝난 추출물에 활성탄은 100 ~ 10,000ppm(맥아 중량 대비) 처리하고 데칸터, Filter Press 및 감압여과 등 3단계 정밀여과 과정을 거쳐 이취와 잡미가 제거된 1차 정제액을 수득하였다. 상기 액상의 1차 정제액을 원심분리기를 이용하여 4차 여과하여 얻은 2차 정제액을 60℃로 감압 농축하여 20 brix 농축액을 90℃에서 30분간 살균하여 수득하여 5차 정밀여과를 통해 최종 맥아엑기스를 수득하였다.1 kg of malt was immersed in two times of purified water and pulverized. The resulting mixture was put into an extraction tank together with 2 g of cellulase (0.2%) and subjected to a primary enzyme treatment at 60 ° C for 2 hours. 20 g (2%) of α-amylase was further added to the mixture after the primary enzyme treatment, and the mixture was heated to 85 ° C. and subjected to secondary enzyme treatment for 3 hours. The activated carbon was treated with 100 ~ 10,000ppm (relative to the weight of malt), and subjected to three-step microfiltration such as decanter, filter press, and reduced pressure filtration to obtain the first purified liquid from which odor and flavor were removed. The liquid first purified solution was subjected to fourth filtration using a centrifuge to obtain a second purified solution, which was then concentrated under reduced pressure at 60 DEG C to obtain a 20 brix concentrate, which was sterilized at 90 DEG C for 30 minutes. The extract was obtained.
비교예Comparative Example 1 : 추가 효소 첨가 없는 경우 1: When no additional enzyme is added
맥아 1kg을 정제수 2배수에 침지 후 분쇄한 것을 추출탱크에 투입을 하여 65℃에서 7시간 가열 추출하였다. 가열이 끝난 추출물을 활성탄은 100 ~ 10,000ppm(맥아 중량 대비) 처리하고 데칸터, Filter Press 및 감압여과 등 3단계 정밀여과 과정을 거쳐 이취와 잡미가 제거된 1차 정제액을 수득하였다. 상기 액상의 1차 정제액을 원심분리기를 이용하여 4차 여과하여 얻은 2차 정제액을 60℃로 감압 농축하여 20 brix 농축액을 90℃에서 30분간 살균하여 수득하여 5차 정밀여과를 통해 최종 맥아엑기스를 수득하였다.1 kg of malt was immersed in 2 times of purified water and pulverized. The resulting mixture was put into an extraction tank and heated at 65 ° C for 7 hours. The heated extract was treated with 100 ~ 10,000ppm (relative to malt weight) of activated carbon and subjected to three-step microfiltration such as decanter, filter press, and reduced pressure filtration to obtain a first purified liquid in which odor and flavor were removed. The liquid first purified solution was subjected to fourth filtration using a centrifuge to obtain a second purified solution, which was then concentrated under reduced pressure at 60 DEG C to obtain a 20 brix concentrate, which was sterilized at 90 DEG C for 30 minutes. The extract was obtained.
비교예Comparative Example 2 : 액화효소만 처리한 경우 2: When only liquefying enzyme is treated
맥아 1kg을 정제수 2배수에 침지 후 분쇄한 것을 추출탱크에 α-아밀라아제 30g (3%)와 함께 투입을 하여 85에서 5시간 동안 효소처리를 하였다. 효소처리가 끝난 추출물을 활성탄은 100 ~ 10,000ppm(맥아 중량 대비) 처리하고 데칸터, Filter Press 및 감압여과 등 3단계 정밀여과 과정을 거쳐 이취와 잡미가 제거된 1차 정제액을 수득하였다. 상기 액상의 1차 정제액을 원심분리기를 이용하여 4차 여과하여 얻은 2차 정제액을 60℃로 감압 농축하여 20 brix 농축액을 90℃에서 30분간 살균하여 수득하여 5차 정밀여과를 통해 최종 맥아엑기스를 수득하였다.1 kg of malt was immersed in 2 times of purified water and pulverized and added to 30 g (3%) of α-amylase in the extraction tank, followed by enzyme treatment at 85 for 5 hours. The enzyme-treated extract was treated with 100 to 10,000 ppm (by weight of malt) of activated carbon and subjected to three-step microfiltration such as decanter, filter press, and reduced pressure filtration to obtain a first purified liquid in which odor and flavor were removed. The liquid first purified solution was subjected to fourth filtration using a centrifuge to obtain a second purified solution, which was then concentrated under reduced pressure at 60 DEG C to obtain a 20 brix concentrate, which was sterilized at 90 DEG C for 30 minutes. The extract was obtained.
비교예Comparative Example 3 : 액화효소만 처리한 경우 3: Only liquefied enzyme is treated
맥아 1kg을 정제수 2배수에 침지 후 분쇄한 것을 추출탱크에 α-아밀라아제 30g (3%)와 함께 투입을 하여 70℃에서 5시간 동안 효소처리를 하였다. 효소처리가 끝난 추출물을 활성탄은 100 ~ 10,000ppm(맥아 중량 대비) 처리하고 데칸터, Filter Press 및 감압여과 등 3단계 정밀여과 과정을 거쳐 이취와 잡미가 제거된 1차 정제액을 수득하였다. 상기 액상의 1차 정제액을 원심분리기를 이용하여 4차 여과하여 얻은 2차 정제액을 60℃로 감압 농축하여 20 brix 농축액을 90℃에서 30분간 살균하여 수득하여 5차 정밀여과를 통해 최종 맥아엑기스를 수득하였다.1 kg of malt was immersed in two times of purified water and pulverized. The resulting mixture was added to the extraction tank together with 30 g (3%) of? -Amylase, followed by enzymatic treatment at 70 ° C for 5 hours. The enzyme-treated extract was treated with 100 to 10,000 ppm (by weight of malt) of activated carbon and subjected to three-step microfiltration such as decanter, filter press, and reduced pressure filtration to obtain a first purified liquid in which odor and flavor were removed. The liquid first purified solution was subjected to fourth filtration using a centrifuge to obtain a second purified solution, which was then concentrated under reduced pressure at 60 DEG C to obtain a 20 brix concentrate, which was sterilized at 90 DEG C for 30 minutes. The extract was obtained.
비교예Comparative Example 4 : 섬유소분해효소만 처리한 경우 4: When only cellulose decomposing enzyme is treated
맥아 1kg을 정제수 2배수에 침지 후 분쇄한 것을 추출탱크에 셀룰라아제 2g (0.2%)와 함께 투입을 하여 60에서 2시간 동안 효소처리를 하였다. 효소처리가 끝난 추출물을 활성탄은 100 ~ 10,000ppm(맥아 중량 대비) 처리하고 데칸터, Filter Press 및 감압여과 등 3단계 정밀여과 과정을 거쳐 이취와 잡미가 제거된 1차 정제액을 수득하였다. 상기 액상의 1차 정제액을 원심분리기를 이용하여 4차 여과하여 얻은 2차 정제액을 60℃로 감압 농축하여 20 brix 농축액을 90℃에서 30분간 살균하여 수득하여 5차 정밀여과를 통해 최종 맥아엑기스를 수득하였다.1 kg of malt was immersed in 2 times of purified water and pulverized. Then, 2 g (0.2%) of cellulase was added to the extraction tank and enzyme treatment was performed at 60 for 2 hours. The enzyme-treated extract was treated with 100 to 10,000 ppm (by weight of malt) of activated carbon and subjected to three-step microfiltration such as decanter, filter press, and reduced pressure filtration to obtain a first purified liquid in which odor and flavor were removed. The liquid first purified solution was subjected to fourth filtration using a centrifuge to obtain a second purified solution, which was then concentrated under reduced pressure at 60 DEG C to obtain a 20 brix concentrate, which was sterilized at 90 DEG C for 30 minutes. The extract was obtained.
비교예Comparative Example 5 : 섬유소분해효소만 처리한 경우 5: When only cellulose decomposing enzyme is treated
맥아 1kg을 정제수 2배수에 침지 후 분쇄한 것을 추출탱크에 셀룰라아제 8g (0.8%)와 함께 투입을 하여 60에서 2시간 동안 효소처리를 하였다. 효소처리가 끝난 추출물을 활성탄은 100 ~ 10,000ppm(맥아 중량 대비) 처리하고 데칸터, Filter Press 및 감압여과 등 3단계 정밀여과 과정을 거쳐 이취와 잡미가 제거된 1차 정제액을 수득하였다. 상기 액상의 1차 정제액을 원심분리기를 이용하여 4차 여과하여 얻은 2차 정제액을 60℃로 감압 농축하여 20 brix 농축액을 90℃에서 30분간 살균하여 수득하여 5차 정밀여과를 통해 최종 맥아엑기스를 수득하였다.1 kg of malt was immersed in 2 times of purified water and pulverized. Then, 8 g (0.8%) of cellulase was added to the extraction tank and enzyme treatment was performed at 60 for 2 hours. The enzyme-treated extract was treated with 100 to 10,000 ppm (by weight of malt) of activated carbon and subjected to three-step microfiltration such as decanter, filter press, and reduced pressure filtration to obtain a first purified liquid in which odor and flavor were removed. The liquid first purified solution was subjected to fourth filtration using a centrifuge to obtain a second purified solution, which was then concentrated under reduced pressure at 60 DEG C to obtain a 20 brix concentrate, which was sterilized at 90 DEG C for 30 minutes. The extract was obtained.
비교예6Comparative Example 6 : 섬유소분해효소 → 액화효소 순서로 처리한 경우 : Cellulose degrading enzyme → liquefying enzyme treatment
맥아 1kg을 정제수 2배수에 침지 후 분쇄한 것을 추출탱크에 셀룰라아제 20g (2%)와 함께 투입을 하여 45에서 2시간 동안 효소처리를 하였다. 이후 α-아밀라아제 30g (3%)와 함께 투입을 하여 85에서 5시간 동안 효소처리를 하였다. 효소처리가 끝난 추출물을 활성탄은 100 ~ 10,000ppm(맥아 중량 대비) 처리하고 데칸터, Filter Press 및 감압여과 등 3단계 정밀여과 과정을 거쳐 이취와 잡미가 제거된 1차 정제액을 수득하였다. 상기 액상의 1차 정제액을 원심분리기를 이용하여 4차 여과하여 얻은 2차 정제액을 60℃로 감압 농축하여 20 brix 농축액을 90℃에서 30분간 살균하여 수득하여 5차 정밀여과를 통해 최종 맥아엑기스를 수득하였다.1 kg of malt was immersed in 2 times of purified water and pulverized. Then, 20 g (2%) of cellulase was added to the extraction tank and the mixture was subjected to enzyme treatment at 45 for 2 hours. Amylase was then added with 30 g (3%) of α-amylase and the enzyme was treated at 85 for 5 hours. The enzyme-treated extract was treated with 100 to 10,000 ppm (by weight of malt) of activated carbon and subjected to three-step microfiltration such as decanter, filter press, and reduced pressure filtration to obtain a first purified liquid in which odor and flavor were removed. The liquid first purified solution was subjected to fourth filtration using a centrifuge to obtain a second purified solution, which was then concentrated under reduced pressure at 60 DEG C to obtain a 20 brix concentrate, which was sterilized at 90 DEG C for 30 minutes. The extract was obtained.
비교예7Comparative Example 7 : 액화효소 → 섬유소분해효소 순서로 처리한 경우 : Liquefying enzyme → treated with enzymatic degrading enzymes
맥아 1kg을 정제수 2배수에 침지 후 분쇄한 것을 추출탱크에 α-아밀라아제 30g (3%)와 함께 투입을 하여 85에서 5시간 동안 효소처리를 하였다. 이후 셀룰라아제 20g (2%)와 함께 투입을 하여 45에서 2시간 동안 효소처리를 하였다. 효소처리가 끝난 추출물을 활성탄은 100 ~ 10,000ppm(맥아 중량 대비) 처리하고 데칸터, Filter Press 및 감압여과 등 3단계 정밀여과 과정을 거쳐 이취와 잡미가 제거된 1차 정제액을 수득하였다. 상기 액상의 1차 정제액을 원심분리기를 이용하여 4차 여과하여 얻은 2차 정제액을 60℃로 감압 농축하여 20 brix 농축액을 90℃에서 30분간 살균하여 수득하여 5차 정밀여과를 통해 최종 맥아엑기스를 수득하였다.1 kg of malt was immersed in 2 times of purified water and pulverized and added to 30 g (3%) of α-amylase in the extraction tank, followed by enzyme treatment at 85 for 5 hours. Subsequently, the cellulase was added with 20 g (2%) of the cellulase and the enzyme treatment was performed at 45 for 2 hours. The enzyme-treated extract was treated with 100 to 10,000 ppm (by weight of malt) of activated carbon and subjected to three-step microfiltration such as decanter, filter press, and reduced pressure filtration to obtain a first purified liquid in which odor and flavor were removed. The liquid first purified solution was subjected to fourth filtration using a centrifuge to obtain a second purified solution, which was then concentrated under reduced pressure at 60 DEG C to obtain a 20 brix concentrate, which was sterilized at 90 DEG C for 30 minutes. The extract was obtained.
시험예Test Example 1 : One : 맥아엑기스의Malt extract 당류 함량 측정 Sugar content measurement
상기 실시예1, 비교예1 내지 7에 의해 제조된 맥아엑기스의 당류를 분석하였다. 상기 당류 분석은 고속액체크로마토그래피를 사용하였고, 고속액체크로마토그래피의 분석 조건은 아래의 표 1과 같았다.The saccharides of the malt extract prepared in Example 1 and Comparative Examples 1 to 7 were analyzed. The saccharides were analyzed by high performance liquid chromatography and the analysis conditions of high performance liquid chromatography were as shown in Table 1 below.
[표 1][Table 1]
실시예 1, 비교예 1 내지 7에서 수득한 맥아엑기스의 당류 총 함량을 측정한 결과를 하기 표 2에 나타내었다.The results of measuring the total sugar content of the malt extracts obtained in Example 1 and Comparative Examples 1 to 7 are shown in Table 2 below.
[표 2] [Table 2]
상기 표 2에서 볼 수 있듯이, 실시예 1은 7.10 로 비교예 1 내지 7까지 당류함량대비 낮은 수준으로 측정되었다. 상기와 같이 실시예 1에 따른 맥아엑기스의 당류 함량이 비교예들에 비해 매우 낮다는 것을 알 수 있었다.As can be seen in Table 2 above, Example 1 was determined to be 7.10 and to a lower level of saccharide content than Comparative Examples 1-7. As described above, it was found that the saccharide content of the malt extract according to Example 1 was much lower than that of the comparative examples.
실시예2 :무알코올Example 2: Non-alcohol 맥주맛Beer flavor 음료 제조 Beverage Manufacturing
맥아 1kg을 정제수 2배수에 침지 후 분쇄한 것을 추출탱크에 셀룰라아제 2g(0.2%)와 함께 투입을 하여 60℃에서 2시간 동안 1차 효소처리를 하였다. 1차 효소처리 후 α-아밀라아제 20g(2%)를 추가 투입하여 85℃로 승온하여 3시간 동안 2차 효소처리를 하였다. 효소처리가 끝난 추출물에 활성탄은 100 ~ 10,000ppm(맥아 중량 대비) 처리하고 데칸터, Filter Press 및 감압여과 등 3단계 정밀여과 과정을 거쳐 이취와 잡미가 제거된 1차 정제액을 수득하였다. 상기 액상의 1차 정제액을 원심분리기를 이용하여 4차 여과하여 얻은 2차 정제액을 60℃로 감압 농축하여 20 brix 농축액을 90℃에서 30분간 살균하여 수득하여 5차 정밀여과를 통해 최종 맥아엑기스를 수득하였다.1 kg of malt was immersed in two times of purified water and pulverized. The resulting mixture was put into an extraction tank together with 2 g of cellulase (0.2%) and subjected to a primary enzyme treatment at 60 ° C for 2 hours. 20 g (2%) of α-amylase was further added to the mixture after the primary enzyme treatment, and the mixture was heated to 85 ° C. and subjected to secondary enzyme treatment for 3 hours. The activated carbon was treated with 100 ~ 10,000ppm (relative to the weight of malt), and subjected to three-step microfiltration such as decanter, filter press, and reduced pressure filtration to obtain the first purified liquid from which odor and flavor were removed. The liquid first purified solution was subjected to fourth filtration using a centrifuge to obtain a second purified solution, which was then concentrated under reduced pressure at 60 DEG C to obtain a 20 brix concentrate, which was sterilized at 90 DEG C for 30 minutes. The extract was obtained.
상기 맥아엑기스와 덱스트린, 대두단백분해물, 대두다당류, 및 쓴맛 물질을 혼합하여 여과 공정을 통해 여과액을 수득하였다. 상기 여과액에 탄산을 혼합하여 용기에 밀봉한 후 살균하여 무알코올 맥주맛 음료를 수득하였다.The malt extract, dextrin, soybean protein degradation product, soy polysaccharide, and bitter taste material were mixed and filtered to obtain a filtrate. The filtrate was mixed with carbonic acid, sealed in a container, and sterilized to obtain a non-alcoholic beer flavored drink.
비교예Comparative Example 8 : 8 : 맥아엑기스Malt extract 첨가 없는 경우 Without addition
덱스트린, 대두단백분해물, 대두다당류 및 쓴맛 물질을 혼합하여 여과 공정을 통해 여과액을 수득하였다. 상기 여과액에 탄산을 혼합하여 용기에 밀봉한 후 살균하여 무알코올 맥주맛 음료를 수득하였다. The dextrin, the soybean protein decomposition product, the soy polysaccharide and the bitter substance were mixed and the filtrate was obtained through the filtration process. The filtrate was mixed with carbonic acid, sealed in a container, and sterilized to obtain a non-alcoholic beer flavored drink.
비교예9Comparative Example 9 : 효소 처리 공정이 포함되지 않은 : Enzyme treatment not included 맥아엑기스를Malt extract 첨가한 경우 When added
맥아 1kg을 정제수 2배수에 침지 후 분쇄한 것을 추출탱크에 투입을 하여 65℃에서 7시간 가열 추출하였다. 가열이 끝난 추출물을 효소처리가 끝난 추출물에 활성탄은 100 ~ 10,000ppm(맥아 중량 대비) 처리하고 데칸터, Filter Press 및 감압여과 등 3단계 정밀여과 과정을 거쳐 이취와 잡미가 제거된 1차 정제액을 수득하였다. 상기 액상의 1차 정제액을 원심분리기를 이용하여 4차 여과하여 얻은 2차 정제액을 60℃로 감압 농축하여 20 brix 농축액을 90℃에서 30분간 살균하여 수득하여 5차 정밀여과를 통해 최종 맥아엑기스를 수득하였다.1 kg of malt was immersed in 2 times of purified water and pulverized. The resulting mixture was put into an extraction tank and heated at 65 ° C for 7 hours. Activated charcoal is treated with 100 ~ 10,000ppm (relative to the weight of malt) to the extracted extract, which has been treated with the enzyme, and subjected to three stages of microfiltration such as decanter, filter press and vacuum filtration, ≪ / RTI > The liquid first purified solution was subjected to fourth filtration using a centrifuge to obtain a second purified solution, which was then concentrated under reduced pressure at 60 DEG C to obtain a 20 brix concentrate, which was sterilized at 90 DEG C for 30 minutes. The extract was obtained.
수득한 맥아엑기스와 덱스트린, 대두단백분해물, 대두다당류 및 쓴맛 물질을 혼합하여 여과 공정을 통해 여과액을 수득하였다. 상기 여과액에 탄산을 혼합하여 용기에 밀봉한 후 살균하여 무알코올 맥주맛 음료를 수득하였다.The resulting malt extract, dextrin, soy protein degradation product, soy polysaccharide and bitter taste substance were mixed and filtered to obtain a filtrate. The filtrate was mixed with carbonic acid, sealed in a container, and sterilized to obtain a non-alcoholic beer flavored drink.
비교예10Comparative Example 10 : 액화효소만 처리한 : Liquefied enzyme only 맥아엑기스를Malt extract 첨가한 경우 When added
맥아 1kg을 정제수 2배수에 침지 후 분쇄한 것을 추출탱크에 α-아밀라아제 30g (3%)와 함께 투입을 하여 60에서 5시간 동안 효소처리를 하였다. 효소처리가 끝난 추출물에 활성탄은 100 ~ 10,000ppm(맥아 중량 대비) 처리하고 데칸터, Filter Press 및 감압여과 등 3단계 정밀여과 과정을 거쳐 이취와 잡미가 제거된 1차 정제액을 수득하였다. 상기 액상의 1차 정제액을 원심분리기를 이용하여 4차 여과하여 얻은 2차 정제액을 60℃로 감압 농축하여 20 brix 농축액을 90℃에서 30분간 살균하여 수득하여 5차 정밀여과를 통해 최종 맥아엑기스를 수득하였다.1 kg of malt was immersed in 2 times of purified water and pulverized. Then, 30 g (3%) of α-amylase was added to the extraction tank and the mixture was subjected to enzymatic treatment at 60 ° C. for 5 hours. The activated carbon was treated with 100 ~ 10,000ppm (relative to the weight of malt), and subjected to three-step microfiltration such as decanter, filter press, and reduced pressure filtration to obtain the first purified liquid from which odor and flavor were removed. The liquid first purified solution was subjected to fourth filtration using a centrifuge to obtain a second purified solution, which was then concentrated under reduced pressure at 60 DEG C to obtain a 20 brix concentrate, which was sterilized at 90 DEG C for 30 minutes. The extract was obtained.
수득한 맥아엑기스와 덱스트린, 대두단백분해물, 대두다당류 및 쓴맛 물질을 혼합하여 여과 공정을 통해 여과액을 수득하였다. 상기 여과액에 탄산을 혼합하여 용기에 밀봉한 후 살균하여 무알코올 맥주맛 음료를 수득하였다.The resulting malt extract, dextrin, soy protein degradation product, soy polysaccharide and bitter taste substance were mixed and filtered to obtain a filtrate. The filtrate was mixed with carbonic acid, sealed in a container, and sterilized to obtain a non-alcoholic beer flavored drink.
시험예Test Example 2 : 2 : 무알코올Non alcohol 맥주맛Beer flavor 음료 관능평가 결과 Results of beverage sensory evaluation
상기 실시예2, 비교예8, 비교예9, 및 비교예10에 따라 제조된 무알코올 맥주맛 음료에 대하여 훈련된 검사 요원 60명을 대상으로 전반적 만족도, 맥주 유사 풍미, 맛, 바디감, 부드러움, 깔끔함, 고미, 외형(거품, 색상 등), 및 향기 등 9 가지 항목에 대한 선호도 평가를 하였다. 선호도 평가는 9점 척도로 진행하였다.The results of this study were as follows: 60 non-alcoholic beverage beverage drinkers prepared according to the example 2, the comparative example 8, the comparative example 9 and the comparative example 10 were tested for general satisfaction, beer-like flavor, taste, body, (Bubble, color, etc.), And fragrance. The preference evaluation was conducted on a 9 point scale.
관능선호도는 순위합으로 표현하였다. 순위합은 상시 실시예2, 비교예8, 비교예9, 및 비교예10에 따라 제조된 무알코올 맥주맛 음료를 각각 시음한 후, 관능적 선호도를 순위(1위 : 1점, 2위 : 2점, 3위 : 3점, 4위 : 4점)로 나타내고 순위에 따른 점수를 합산하여 산출하였다.The sensory preference was expressed as rank sum. The non-alcohol beer flavored beverages prepared in accordance with Examples 2, 3, 4, 5, and 10 were respectively tasted, and their sensory preferences were ranked 1 Points, 3 points: 3 points, 4 points: 4 points), and the scores according to the rankings were added up.
상기 9가지 항목은 점수가 클수록 선호도가 높음을 의미하고, 순위합은 낮을수록 우수함을 의미한다. 그 결과는 아래의 표 3과 같았다.The above nine items indicate that the higher the score, the higher the preference, and the lower the rank sum, the better. The results are shown in Table 3 below.
[표 3][Table 3]
상기 표 3을 참조하면, 모든 평가 항목에서 실시예 2에 따른 무알코올 맥주맛 음료가 비교예 8, 비교에 9, 및 비교예10 에 따라 제조된 무알코올 맥주맛 음료에 비해 좋은 결과를 보임을 알 수 있다.Referring to Table 3 above, the non-alcoholic beer flavored beverage according to Example 2 in all the evaluation items showed a better result than the non-alcoholic beer flavored beverage prepared according to Comparative Example 8, Comparative Example 9, and Comparative Example 10 Able to know.
상기와 같이 본 발명의 실시예 2에 따른 무알코올 맥주맛 음료의 관능평가 결과 비교예들에 비해 매우 높은 수준의 관능적 품질을 가진다는 것을 알 수 있었다.As described above, the sensory evaluation of the non-alcoholic beer-flavored beverage according to Example 2 of the present invention showed a very high level of sensory quality as compared with the comparative examples.
본 발명에 따른 저감미 맥아엑기스의 제조방법에 의하면, 잡미와 이취가 제거되고, 단당류와 이당류의 함량이 낮아 기존 맥아엑기스보다 감미가 낮으며, 맥주 특유의 풍부한 바디감을 가지면서 깔끔하고 풍미가 향상된 무알코올 맥주맛 음료를 제조할 수 있다는 점을 알 수 있었다.According to the method for producing the reduced malt extract according to the present invention, the smell and odor are removed, the content of the monosaccharide and the disaccharide is low and the sweetness is lower than that of the malt extract, and a clean and flavor- Alcohol beer-flavored beverage.
이상으로 본 발명 내용의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적 기술은 단지 바람직한 실시 양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다고 할 것이다.While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereby. something to do. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.
Claims (23)
상기 1차 효소처리한 맥아를 α-아밀라아제(amylase)로 2차 효소처리 하는 단계; 및
상기 2차 효소처리한 맥아를 활성탄 처리, 정제, 및 농축하는 단계를 포함하는 저감미 맥아엑기스의 제조방법.
Treating the malt with a cellulase as a primary enzyme;
Treating the primary enzyme-treated malt with a secondary enzyme with? -Amylase; And
And treating the malt treated with the secondary enzyme with activated carbon, refining, and concentrating the malt.
The method according to claim 1, wherein the primary enzyme treatment is carried out at 40 to 85 캜 for 1 to 5 hours.
The method according to claim 1, wherein the secondary enzyme treatment is performed at 45 to 95 캜 for 1 to 7 hours.
The method according to claim 1, wherein the cellulase is added in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of the malt.
The method according to claim 1, wherein the? -Amylase is added in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of the malt.
The method according to claim 1, wherein the activated carbon is treated at a concentration of 100 to 10,000 ppm based on the weight of the malt for 0.5 to 3 hours at room temperature.
A reduced-malt extract prepared by the method of any one of claims 1 to 6, wherein the total malt extract (20 ° Bx standard) contains 3 to 15% by weight of monosaccharides and disaccharides.
A flavor-enhanced non-alcoholic beer-flavored drink comprising an extract powder containing the reduced malt extract of claim 7.
[9] The beverage according to claim 8, wherein the malt extract comprises 0.03 to 15% by weight of the non-alcoholic beer flavored beverage.
The beverage according to claim 8, wherein the content of monosaccharides and disaccharides in the non-alcoholic beer flavored beverage is 0.5 g / 100 ml or less.
[9] The beverage according to claim 8, wherein the extract powder comprises 2 to 4% by weight of non-alcoholic beer flavored beverages.
9. The flavorless beverage according to claim 8, wherein the extract has improved flavor, including dextrin, a foaming agent, a soy polysaccharide, and a bitter substance.
The beverage according to claim 12, wherein the dextrin is at least one selected from the group consisting of indigestible maltodextrin, maltodextrin, and polydextrose.
[12] The beverage according to claim 12, wherein the dextrin comprises 10 to 85% by weight of the whole extract.
13. The beverage according to claim 12, wherein the bubbling agent is a flavorless alcohol-free beer flavored beverage.
[16] The beverage according to claim 15, wherein the beef protein degradation product is a soybean peptide or pea peptide.
[12] The beverage according to claim 12, wherein the foaming agent comprises 0.1 to 20% by weight of the whole extract.
[12] The beverage according to claim 12, wherein the soy polysaccharide comprises 0.1 to 20% by weight of the whole extract.
13. The flavor enhancer of claim 12, wherein the NIBEM is 50 or more.
The beverage according to claim 12, wherein the bitter taste substance is iso-αacid.
The beverage according to claim 20, wherein the iso-αacid concentration is 0.0001 g / L or more.
The flavorless alcohol-free beer flavored drink according to claim 8, wherein the content of saccharides is 0.01 to 0.5% by weight per 100 ml, and the calories per 100 ml are 0.1 to 20 kcal.
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