KR20180108216A - substance mixing device - Google Patents

Abstract

Disclosed is a device for mixing a substance. According to one aspect of the present invention, a device of mixing a substance comprises: a first multi-wall plate having a plurality of first walls, each of which stores a first sample therein and includes one opened side; a second multi-wall plate disposed on an upper portion of the first multi-wall plate, having a plurality of second walls, each of which has an opened one side and the other side with a non-permeable separator, and stores a second sample therein; a busting unit having a rotary shaft member which rotates at a predetermined angle with respect to the second walls and moves in a vertical direction, and a busting member which is formed on one end portion of the rotary shaft member and busts the separator; and a guide unit having a guide member and a guide housing which guide rotation and vertical movement of the rotary shaft member. The guide member may be formed on any one of the rotary shaft member and the guide housing, and a guide groove corresponding to the guide member may be formed in any one of the rotary shaft member and the guide housing wherein the guide member is coupled to the guide groove to be able to move. According to the present invention, the device of mixing a substance is capable of accelerating a mixing reaction by applying an external force such as a rotation force to a mixture in which different types of sample are mixed.

Description

[0001] The present invention relates to a substance mixing device,

The present invention relates to an apparatus for mixing heterogeneous materials.

Currently, compound tests using multi-well plate-based microplate readers are widely used in universities, companies and research institutes.

The multi-well plate is called a dowel plate or a dowel block, and a multi-well plate having a plurality of independent unit wells is constructed in accordance with a standardized format and is commercially available. For example, 96-well plates or 12X8 arrays are most commonly used.

A microplate reader is a device used to inject a substance into a standardized microplate reader and perform photochemical analysis of the substance, such as absorption fluorescence emission polarization.

In order to inject the substance into the multiwell plate, there is a method of injecting the substance using an injector or manually using a pipette. Thus, there is a problem that only a very limited number of substances can be injected at a desired time, and it is not suitable for drug development or drug screening, which requires testing and searching for tens of thousands of compounds.

According to one embodiment of the present invention, a tester carrying out a chemical biological biochemical test using a device having a plurality of wells or a multi-well plate can rapidly inject compounds ranging from tens of thousands to hundreds of thousands kinds, A material mixing apparatus useful for quickly measuring the reaction is provided.

Further, there is provided a material mixing apparatus for accelerating a mixing reaction by applying an external force such as a rotational force to a mixture of different kinds of samples,

Also, according to an embodiment of the present invention, a multi-well plate-based material mixing apparatus is provided so as to be compatible with a microplate reader.

According to an aspect of the present invention, there is provided a first multiwell plate including a plurality of first wells in which a first sample is accommodated and one side is opened; A second multi-well plate disposed on the upper side of the first multi-well plate, the second multi-well plate including a plurality of second wells in which a non-permeable separation membrane is formed on one side and a second sample is received therein; A rupturing part including a rotating shaft member which rotates at a predetermined angle with respect to the second well and moves up and down, and a rupture member which is formed at one end of the rotating shaft member and ruptures the separating membrane; And a guide part having a guide member and a guide housing for guiding rotation and vertical movement of the rotating shaft member, wherein the guide member is formed on one of the rotation shaft member and the guide housing, And a guide groove in which the guide member is movably coupled is formed in the other of the rotation shaft member and the guide housing.

In this case, the rupture unit may further include a mixing member formed on the rotating shaft member to promote mixing of the first sample and the second sample, and the mixing member may be disposed apart from the rupture member.

In this case, the guide member may include a guide protrusion formed to protrude outward of the rotating shaft member and inserted in the guide groove and moving along the guide groove.

At this time, the guide housing may be disposed on the upper side of the second multi-well plate so as to communicate with the second well.

At this time, both end portions of the guide groove are spaced apart from each other by a predetermined angle, and the rotating shaft member can be moved in the vertical direction while being rotated by the predetermined angle.

At this time, the guide groove may be formed obliquely with respect to the longitudinal direction of the guide housing.

In this case, the rupture portion may further include an elastic member disposed inside the guide housing, and the elastic member may provide an elastic force to the rotating shaft member such that the rotating shaft member is repeatedly moved in the vertical direction.

The support plate may further include a support plate disposed between the guide housing and the second multi-well plate. The support plate may have a support hole through which the rotation axis member is inserted.

At this time, the elastic member is disposed between the guide member and the support plate, and can be supported by the support plate when the rotation axis member and the guide member are moved downward.

At this time, the second multi-well plate may be disposed above the first multi-well plate such that each of the second wells is at least partially inserted into each of the first wells.

Here, the first multiwell plate may include a first connecting member that interconnects the plurality of first wells, and the second multiwell plate may include a second connecting member that interconnects the plurality of second wells And the second connecting member is supported on the upper side of the first connecting member, so that the second multi-well plate can be supported on the first multi-well plate.

In this case, the plurality of first wells may be arranged side by side in the horizontal direction and the longitudinal direction on the same plane, and the plurality of second wells may be provided in the lateral direction and the longitudinal direction on the same plane.

At this time, the rupturing portion may be formed to correspond to the number of the second wells, and each of the rupturing portions may be arranged in the lateral direction and the longitudinal direction on the upper side of the second multi-well plate.

In this case, the driving unit may further include a driving unit that presses the rotating shaft member of the rupturing unit toward the lower side, and the driving unit may include a plurality of rupturing members corresponding to the number of the rupturing members arranged in a row in parallel to each other.

The apparatus may further include a control unit for controlling the moving unit and the first multi-well plate, the second multi-well plate, and the rupture unit in the longitudinal direction, When all of the separation membranes of a plurality of the second wells constituting one column are ruptured, the moving unit moves the first multiwell plate, the second multiwell plate, and the rupture unit to neighboring columns .

According to an exemplary embodiment of the present invention, a compatible material mixing apparatus is provided in a commercially available microplate reader. Through this, the first and second samples to be tested are injected into the first and second multi-well plates arranged up and down, and then the rupture member is operated to rapidly inject the desired compound into each well independently And the reaction can be measured.

Therefore, it can be usefully used for drug screening for the development of new drugs using hundreds of thousands of samples. It can also be used as a new high-speed screening method.

1 is an assembled perspective view of a material mixing apparatus according to an embodiment of the present invention.
FIG. 2 is a combined view of the rupture portion, the guide portion, the first well, and the second well of the material mixing apparatus according to an embodiment of the present invention.
3 is a combined view of a first well and a second well of a material mixing apparatus according to an embodiment of the present invention.
4 is an exploded perspective view of a guide part of a material mixing apparatus according to an embodiment of the present invention.
5 is a cross-sectional view of a material mixing apparatus according to an embodiment of the present invention.
6 is a cross-sectional view illustrating the operation of the material mixing apparatus according to one embodiment of the present invention.
7 is a cross-sectional view illustrating the operation of the material mixing apparatus according to one embodiment of the present invention.
8 is a cross-sectional view of a material mixing apparatus according to another embodiment of the present invention.
9 is a cross-sectional view illustrating the operation of the material mixing apparatus according to another embodiment of the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, which will be readily apparent to those skilled in the art to which the present invention pertains. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and the same or similar components are denoted by the same reference numerals throughout the specification.

In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, components, parts, or combinations thereof.

1 is an assembled perspective view of a material mixing apparatus according to an embodiment of the present invention. FIG. 2 is a combined view of the rupture portion, the guide portion, the first well, and the second well of the material mixing apparatus according to an embodiment of the present invention. 3 is a combined view of a first well and a second well of a material mixing apparatus according to an embodiment of the present invention. 4 is an exploded perspective view of a guide part of a material mixing apparatus according to an embodiment of the present invention. 5 is a cross-sectional view of a material mixing apparatus according to an embodiment of the present invention. 6 is a cross-sectional view illustrating the operation of the material mixing apparatus according to one embodiment of the present invention. 7 is a cross-sectional view illustrating the operation of the material mixing apparatus according to one embodiment of the present invention.

The material mixing apparatus 1 according to an embodiment of the present invention includes a first multi well plate 100, a second multi well plate 200, a rupturing unit 30, a guide unit 40, a driving unit 50, A moving part 60 and a control part.

Referring to FIGS. 1 and 3, the first multi-well plate 100 may be arranged such that a plurality of first wells 10 are arranged side by side and longitudinally on the same plane. The first well 14 is accommodated in the first well 10 and can be disposed on the lower side of the second well 20 with reference to Fig.

At this time, the second wells 200 may be arranged in the horizontal direction and in the longitudinal direction on the same plane on the same plane as the first multi-well plate 100. The second well (20) is accommodated in the second sample (24) and can be disposed on the upper side of the first multi-well plate (100).

Here, the types of the first sample 14 and the second sample 24 are not limited, and they may be plural kinds of samples. In particular, when performing screening for the development of a new drug, the first sample 14 may be a cell material to be assayed for activity, and the second sample 24 may be a candidate for such a cell material activator.

The first multiwell plate 100 may include a first connecting member 12 connecting each of the plurality of first wells 10 to each other and the second multiwell plate 200 may include a plurality of second wells 10, And a second connecting member 22 connecting the first and second connecting members 20 to each other.

Further, the first connecting member and the second connecting member can function as a kind of flange. 3, the second connecting member 22 of the second multi-well plate 200 may be supported on the first connecting member 12 of the first multi-well plate 100. [

That is, the first multi-well plate 100 and the second multi-well plate 200 may be arranged so that the respective second wells 20 are inserted into the respective first wells 10, The connecting member 22 can be supported by the first connecting member 12. [

The diameter of the first well 10 is not particularly limited and the diameter of the second well 20 may be smaller than the diameter of the first well 10 to be inserted into the first well 10 .

The height of the second well 20 may be smaller than the height of the first well 10. This is because it is preferable that the second well 20 is not immersed in the first sample 14 contained in the first well 10.

In an embodiment of the present invention, the plurality of second wells 20 of the second multi-well plate 200 may have openings formed on the upper side and the lower side, respectively, so that both sides thereof are opened.

2, the rupturing member 30 and the rupturable member 30 may be inserted into the upper side and the rupturable member 36 and the rotary shaft member 32 may be inserted into the second well 20 through the upper side opening. Lt; / RTI > Further, the second sample 24 can be injected through the upper side opening.

At this time, a non-permeable separating film 28 may be formed in the lower side opening of the second well 20. The second sample 24 contained in the second well 20 does not flow out to the lower side until physical rupture occurs. The second sample 24 can be received in the second well 20 so that the upper side of the first well 10 in which the first sample 14 is accommodated is isolated by the separating film 28. [

In one embodiment of the present invention, the rupturable portion 30 includes a rupturable member 36 that ruptures the non-permeable membrane 28 formed in the second well 20, a second well A rotating shaft member 32 extending outwardly of the first and second wells 10 and 20 and moving in the vertical direction within the first and second wells 10 and 20, a mixing member 34 formed on the rotating shaft member 32, Member 38 as shown in FIG.

The rupture member 36 is formed at one end of the rotating shaft member 32 and can be moved to the second well 20 through the upper side opening of the second well 20 as shown in Fig.

The rupturable member 36 moves toward the non-permeable separator 28 formed at the lower side opening of the second well 20 when the rotating shaft member 32 moves downward from the inside of the second well 20, ) Can be ruptured.

In this case, the rupturable member 36 may be made of a suitable material to rupture the separation membrane 28 formed in the second well 20, which is generally made of iron, It may be made of stainless steel or the like so as not to rust. However, the rupturing member 36 may be disposed in the second well 20 at a position spaced apart from the second sample 24 so as not to be immersed in the second sample 24.

The rotating shaft member 32 may extend from one side of the rupturing member 36 to the upper side. The rotating shaft member 32 may extend out of the second well 20 and may be urged by a driving unit 50 located outside the second well 20 to rotate the second well 20 together with the rupturable member 36, Can be moved toward the separation membrane (28) side of the well (20).

At this time, one end of the rotating shaft member 32 can be coupled by screwing so as to be detachable from the rupturing member 36. When the rotating shaft member 32 is screwed to the rupturing member 36, can do. Alternatively, the rotatable shaft member 32 may be integrally formed with the rupturable member 36.

In one embodiment of the present invention, the rupturing portion 30 may further include a mixing member 34. [ In order to promote the reaction of the mixed first sample 14 and the second sample 24, a physical external force may be applied using the mixing member 34 in one embodiment of the present invention.

2, the mixing member 34 is formed on the rotating shaft member 32 and may be formed at a position spaced apart from the rupturing member 36 by a predetermined distance.

At this time, the predetermined distance between the mixing member 34 and the rupture member 36 may be an interval at which the mixing member 34 can be sufficiently submerged in the mixture contained in the first well 10.

As a result, when the rupture member 36 formed on the end side of the rotating shaft member 32 moves downward and ruptures the separation membrane 28 of the second well 20,

The first sample 14 and the second sample 24 are mixed in the first well 10 and the mixing member 34 can be immersed in the mixture contained in the first well. The mixing of the mixture can be promoted in the process of returning the rotating shaft member 32 to the upper side by the elastic force of the elastic member 38. [ That is, the mixing member 34 can stir the mixture contained in the first well 10 repeatedly in the vertical direction to promote the reaction.

Referring again to FIG. 2, the mixing member 34 may be formed in a disc shape formed in a radially outward direction of the rotating shaft member 32. When the diameter of the mixing member 34 is too small, If the diameter is too large, there is a problem that the inside of the second well 20 can not be smoothly moved.

Accordingly, the mixing member 34 can be formed to have a size that is smoothly movable within the first well 10 and the second well 20, and at the same time, the mixing member 34 can be sized to promote mixing of the mixture.

The mixing member 34 may be made of a material having an elastic force in the axial direction of the rotating shaft member 32.

In one embodiment of the present invention, the rupturing portion 30 may further include an elastic member 38 that provides an elastic force to the rotating shaft member 32.

2 and 5, the elastic member 38 may be a spring, and one end may be supported by the guide member 44 and the other end may be supported by the support plate 48.

The rupture member 36 and the rotating shaft member 32 which are locked to the first sample can be returned to the upper side again and the driving unit 50 can return the rotating shaft member 32 ) Is pressurized again to move up and down repeatedly. This allows the rupturing member 36 to move repeatedly through the first well 10 and the second well 20 to facilitate mixing of the first sample 14 and the second sample 24. [

The material mixing apparatus 1 according to an embodiment of the present invention may include a guide unit 40 for guiding the rotation of the rupturable member 36.

At this time, the guide portion 40 may include a guide housing 42 having an inner cylindrical shape and a guide member 44 formed on the rotary shaft member 32.

Referring to FIG. 4, the guide housing 42 has a hexahedron outside, and a cylindrical hole may be formed therein. The guide housing 42 may be formed so that one side and the other side of the guide housing 42 are opened as shown in FIG.

2 and 4, the rupture member 36 and the rotating shaft member 32 can be inserted through the upper side opening 45 based on Fig. The lower side opening 47 is aligned to communicate with the second well 20 so that the rupturable member 36 and the rotating shaft member 32 inserted through the guide housing 42 are moved downward to form a second well 20 into the interior of the first well 10.

Referring again to FIG. 2, a guide member 44 may be formed at the other end of the rotating shaft member 32. The guide member 44 may have a disk shape extending in the radially outward direction of the rotation shaft member 32.

At this time, at least one guide protrusion 46 may be formed on the outer side of the guide member 44. Referring to FIG. 2, two guide protrusions 46 may be formed and each protrusion may be disposed 180 degrees apart.

This is because the guide protrusion 46 moving along the guide groove 43 is stably supported.

Referring again to FIG. 4, guide grooves 43 may be formed on the inner surface of the guide housing 42. The guide groove 43 is formed with a path through which the guide protrusion 46 is inserted and moves from the upper side opening 45 to the lower side opening 47 of the guide housing 42.

At this time, the upper side end portion 43a of the guide groove 43 may be formed in the upper side opening 45 of the guide housing 42. 1, when the rupturable member 36 and the rotary shaft member 32 are inserted into the guide housing 42, the guide protrusion 46 of the guide member 44 is inserted into the upper portion of the guide groove 43 Side opening 45, as shown in Fig.

On the other hand, the lower side end portion 43b of the guide groove 43 may not extend to the lower side opening 47 of the guide housing 42. This is because the guide member 44 can not be moved to the lower side opening 47 of the guide housing 42 by the elastic member 38 to be compressed.

At this time, the guide groove 43 may be formed obliquely with respect to the longitudinal direction of the guide housing 42. The obliquely formed is to be rotated by a predetermined angle by the guide groove 43 when the rotating shaft member 32 is moved to the lower side.

In addition, the lower side end portion 43b of the guide groove 43 may be spaced apart from the upper side end portion 43a by a predetermined angle. Here, the predetermined angle may be an angle at which the rotating shaft member 32 is rotated, and preferably between 90 and 180 degrees.

If the angle of rotation of the rotating shaft member 32 is large, mixing of the first sample 14 and the second sample 24 can be further promoted as described later. However, when the rotation angle of the rotation shaft member 32 is determined to be large in consideration of the lengthwise length of the guide housing 42, the rotation of the rotation shaft member 32 may not be smooth due to the downward movement of the rotation shaft member 32.

That is, the rotation angle of the rotary shaft member 32 is set such that the vertical distance between the upper side end portion 43a and the lower side end portion 43b of the guide groove 43 and the vertical distance between the upper side end portion 43a and the lower side end portion 43b Can be determined in consideration of the angle. If the vertical distance between the upper side end portion 43a and the lower side end portion 43b of the guide groove 43 is increased, the spacing angle between the upper side end portion 43a and the lower side end portion 43b may also increase have. As described above, the rotatable angle of the rotating shaft member 32 can be determined to such an extent that it can be rotated along the guide groove 43 when the lower side pressing force is applied. Thereby, the rotatable angle of the rotating shaft member 32 can be increased to enable faster mixing.

However, since the vertical distance between both ends of the guide groove 43 is related to the height of the guide housing 42, it can be determined in consideration of the physical limit of the guide housing 42.

The material mixing apparatus 1 according to an embodiment of the present invention may further include a support plate 48.

At this time, the support plate 48 is disposed on the lower side of the guide housing 42 and can be disposed on the upper side of the second multi-well plate 200. 2 and 4, a support hole 49 may be formed on the support plate 48 to allow the guide housing 42 and the second well 20 to communicate with each other.

Here, the support hole 49 may be formed to have a diameter corresponding to the diameter of the rotation axis member 32 so that the rotation and the rotation of the rotation axis member 32 are supported.

At this time, as described above, when the elastic member 38 is compressed, the elastic member can be supported by the guide member 44 on the upper side and supported by the support plate 48 on the lower side.

The first and second multi-well plates 100 and 200, the rupturing portion 30, and the guide portion 40 have been described above. Hereinafter, the structure of the above-described structures will be described in general with reference to cross-sectional views of FIG. 5 and FIG. 5 is a cross-sectional view showing any one of the first well 10, the second well 20, the guide portion 40, and the rupturing portion 30 shown in FIG.

5, a first sample 14 is accommodated in the first well 10 and a part of the second well 20 is inserted into the first well 10 to such an extent that the second well 20 is not immersed in the first sample 14. [ . The second connection member 12 formed at the outer periphery of the first well 10 and formed at the outer periphery of the second well 20 by the first connection member 12 connecting the first wells 10, 22 can be supported.

At this time, a non-permeable separation membrane 28 is formed in the lower side opening of the second well 20 so that the second sample 24 is isolated from the first sample 14 and is accommodated in the second well 20 .

A support plate 48 may be disposed on the upper side of the second connection member 22 of the second well 20. The support plate 48 may be formed with a support hole 49 for supporting the movement and rotation of the rotation shaft member 32.

The guide housing 42 may be disposed on the upper side of the support plate 48 and may be arranged to communicate from the guide housing 42 through the support hole 49 to the second well 20. A guide groove 43 is formed in the inner side surface of the guide housing 42 and the guide protrusion 46 of the guide member 44 is movably coupled to the guide groove 43.

At this time, an elastic member 38 for providing an elastic force to the rotary shaft member 32 is disposed on the guide member 44 and the support plate 48, and the compressed elastic member 38 moves the guide member 44 to the upper side So that the rotating shaft member 32 can be returned to its original position.

The rupture member 36 may be formed at one end of the rotating shaft member 32 and the other end may extend outside the guide housing 42. At this time, the mixing member 34 may be formed at a position spaced apart from the one rupturing member 36, and the rupturing member 36 and the mixing member 34 may be located on the lower side of the support plate 48, 2 < / RTI >

The material mixing apparatus 1 according to an embodiment of the present invention may further include a driving unit 50.

In this case, the driving unit 50 may be a linear actuator driven in the vertical direction. However, the present invention is not limited thereto, and may be a structure in which a rotary actuator is coupled to a driver assembly that converts a rotary motion into a reciprocating motion. But is not limited to an electric actuator such as a motor, and may be a hydraulic cylinder.

1, the driving unit 50 may include a first multi-well plate 100, a second multi-well plate 200 and a rupturing unit 30 arranged in parallel in the longitudinal direction and the transverse direction. have. In this case, the driving unit 50 may be formed in a plurality of units corresponding to the number of the first and second multi-well plates 100 and 200 arranged in a row in the lateral direction.

Here, the transverse direction means the x-axis direction shown in Fig. 1, and the longitudinal direction means the y-axis direction. Further, C1 means a first row arranged in a row in a row.

At this time, as shown in FIG. 5, an impact piece 52 may further be formed at an output end of the driving unit 50. The impact piece 52 can move the rotating shaft member 32 down by applying an impact to the end portion of the rotating shaft member 32. [

At this time, when the rotary shaft member 32 is moved to the lower side by the impact piece 52, the elastic member 38 supported by the support plate 48 can move the rotary shaft member 32 to the upper side again.

At this time, driving can be repeatedly performed in such a manner that the rotating shaft member 32 returned to the home position is pressed again to the lower side.

The rupture member 36 coupled to one end of the rotating shaft member 32 can provide additional external force to the mixture of the first well 10 and thereby the first sample 14 and the second sample 24 ) Can be achieved more quickly.

The material mixing apparatus 1 according to an embodiment of the present invention may further include a moving unit 60.

At this time, the moving unit 60 can move the first multi-well plate 100, the second multi-well plate 200, the rupturing unit 30, and the guide unit 40 in unison.

1, a driving unit 50 may be disposed on one side of the first and second multi-well plates 100 and 200, and the moving unit 60 may include a first and a second multi-well plates 100 and 200 To the driving unit 50 side.

8, 8 wells and 8 rupturable parts 30 are formed in the first column C1 and impact pieces 52 of the driving part 50 are arranged on the upper side of the rupturable part 30. [ At this time, the impact piece 52 of the driving unit 50 presses the rotating shaft member 32 to rupture the separation membrane 28 of the second well 20 to form the first sample 14 of the first column C1, And the second sample 24 can be mixed.

At this time, when all of the separation membranes 28 of the first column C1 are ruptured, the moving section 60 is moved so that the second row C2 is positioned below the impact piece 52 of the driving section 50. In this case, The plate and the rupturing portion 30 can be moved.

The material mixing apparatus 1 according to an embodiment of the present invention may further include a control unit.

The controller may further include a sensor capable of detecting whether the first sample 14 and the second sample 24 are mixed to cause a reaction. When the reaction of the first column C1 is completed, the control unit moves the multi-well plate so that the rupturing unit 30 of the second row C2 is positioned below the impact piece 52 .

5 and 6, the operation of the material mixing apparatus 1 according to the embodiment of the present invention will be described below.

5, the guide member 44 formed on the rotary shaft member 32 can be coupled to the guide groove 43 on the inner side of the guide housing 42. [ At this time, the elastic member 38 may be disposed between the guide member 44 and the support plate 48 in an uncompressed state, and the second sample 24 of the second well 20 may be disposed between the separation membrane 28 The first sample 14 and the second sample 14 can be separated and accommodated.

As shown in Fig. 6, the impact piece 52 of the driving unit 50 can press the rotating shaft member 32 downward. The guide protrusion 46 of the guide member 44 formed on the rotating shaft member 32 can be moved downward along the guide groove 43. At this time, since the guide groove 43 is formed obliquely with respect to the longitudinal direction of the guide housing 42, the guide member 44 can be lowered while being rotated by a predetermined angle.

The rupturable member 36 and the rupturable member 36 can also be lowered while being rotated by a predetermined angle and the rupturable member 36 is lowered toward the first well 10, The separation membrane 28 of the well 20 can be ruptured. Thereafter, the first sample 14 and the second sample 24 are mixed in the first well 10 and then the rotating shaft member 32 and the rupturable member 36 are further rotated inside the first well 10 .

The mixing and reaction of the first sample 14 and the second sample 24 can be further promoted by the rupturing member 36 rotating in the first well 10. The mixing member 34 can further promote the mixing of the first sample 14 and the second sample 24 by pressing the mixture in the first well 10 along with the descent of the rotating shaft member 32 .

The compressed elastic member 38 disposed between the guide member 44 and the support plate 48 can move the guide member 44 to the upper side in accordance with the descent of the rotary shaft member 32. [ The rupturing member 36 can be rotated in the opposite direction by a rotated angle when the rotating shaft member 32 is moved downward while the rotating shaft member 32 is moved to the upper side again. The mixing of the first sample 14 and the second sample 24 can be promoted as the rotating shaft member 32 and the rupturable member 36 are rotated and raised.

The rupturable member 36 and the mixing member 34 pass through the ruptured separation membrane 28 of the second well 20 and then through the ruptured separation membrane 28 of the second well 20, 1 < / RTI > well 10 to provide mixing and reaction of the mixture.

Referring to FIG. 7, a first well 10 arranged side by side in the lateral direction of the first multi-well plate 100 is shown, wherein C1 is referred to as a first column and C2 is referred to as a second column. The term "column" as used herein refers not only to the first well 10 parallel to the lateral direction of the first multiwell plate 100 but also to the second well 20 corresponding to the first well 10, The rupturing portion 30 and the guide portion 40, which are respectively disposed on the upper side of the guide portion 20, are referred to together.

The first column C1 of FIG. 7 represents the first longitudinal row of the first multi-well plate 100 and the corresponding first column of the multi-well plate 200 and the first column of the first row of the second multi- (30) and a guide portion (40) arranged on the side of the guide portion (40).

At this time, when all the separation membranes 28 of the second well 20 constituting the first row C1 of the first multi-well plate 200 and the second multi-well plate 200 are ruptured, It is possible to move the multiwell plate so that the second row C2 is disposed below the impact piece 52 of the driving unit 50 by controlling the controller 60. [

Hereinafter, a material mixing apparatus according to another embodiment of the present invention will be described with reference to FIGS. 8 and 9. FIG.

8 is a cross-sectional view of a material mixing apparatus according to another embodiment of the present invention. 9 is a cross-sectional view illustrating the operation of the material mixing apparatus according to another embodiment of the present invention.

The material mixing apparatus according to another embodiment includes a first multi-well plate 100, a second multi-well plate 200, a rupturable portion 30, a support portion 70, a driving portion 50, a moving portion 60, . ≪ / RTI > In this case, since the first multi-well plate, the second multi-well plate, the driving unit, the moving unit, and the control unit are as described above, the description is omitted.

The material mixing apparatus according to another embodiment of the present invention is a material mixing apparatus in which the rupture member 36 moves vertically without rotating to rupture the separation membrane 28 of the second well 20, A supporting portion 70 for guiding the up and down movement of the supporting rod member 72 and the supporting rod member 72 to be engaged and an elastic member 74 for providing an elastic force to the supporting rod member.

In another embodiment of the present invention, the support 70 may include a first support plate 73a and a second support plate 73b and may be located on the upper side of the first and second wells 10,20 . At this time, the first support plate 73a is located on the upper side of the first and second wells, and the second support plate 73 is located on the upper side of the first support plate 73b.

According to another embodiment of the present invention, the first and second support plates 73a and 73b are arranged such that two plates are parallel to each other while a support rod member 72 is vertically inserted into the first and second support plates 723a and 73b A through hole 71 may be formed so as to be movable. The two plates are provided with two through holes 71 arranged in parallel in the vertical direction, and the supporting rod member 72 inserted therein can stably move up and down without shaking in the lateral direction.

The two support plates 73a and 73b may be spaced apart. An elastic member 74, which provides an elastic force to the support rod member 72, may be added to the spaced-apart space between the two support plates.

The resilient member 74 can provide sufficient resilience when the support bar member 72 is compressed as it ruptures the membrane of the second well 20 and returns to its equilibrium position after rupture. A tension spring may be generally included and one end of the tension spring is coupled to the outward end of the support rod member 72 and the other end is connected to the first support plate 73a located below the first and second support plates 73a and 73b Can be combined.

The upper portion of the support bar member 72 can be manually operated by a user. For this purpose, a pushing portion 75 may be formed on the upper portion of the support bar member 72.

In another embodiment of the present invention, the driving portion 50 can press the pressing portion 75 of the supporting rod member 72. The support member 72 and the rupture member 36 formed at the end portion move to the lower side and the rupture member 28 of the second well 20 can rupture as the drive unit 50 is pressed have.

When the separation membrane 28 ruptures, the second sample may be injected into the first well 10 and mixed with the first sample. At this time, the elastic member 74 formed on the supporting rod member 72 is compressed, and the elastic member 74 provides an elastic force to push the supporting rod member 72 upward again. The driving unit 50 can further pressurize the pressing part 75 of the supporting rod member 72 returned to the home position to repeatedly promote the mixing of the mixture in the first well 10. [

The material mixing apparatus 1 according to the exemplary embodiment of the present invention can rapidly measure various kinds of samples by mixing them.

This can be used as a new high throughput high-speed screening device which can be useful for drug screening for development of new drugs using hundreds of thousands of samples.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the scope of the present invention is not limited to the disclosed exemplary embodiments, Other embodiments may easily be suggested by adding, changing, deleting, adding, or the like of a component, but this also belongs to the scope of the present invention.

1: material mixing apparatus 100: first multi-well plate
200: second multi-well plate 30: rupture part
40: guide portion 50:

Claims (15)

A first multiwell plate including a plurality of first wells in which a first sample is accommodated and one side is opened;
A second multi-well plate disposed on the upper side of the first multi-well plate, the second multi-well plate including a plurality of second wells in which a non-permeable separation membrane is formed on one side and a second sample is received therein;
A rupturing part including a rotating shaft member which rotates at a predetermined angle with respect to the second well and moves up and down, and a rupture member which is formed at one end of the rotating shaft member and ruptures the separating membrane; And
And a guide portion having a guide member and a guide housing for guiding rotation and vertical movement of the rotating shaft member,
Wherein the guide member is formed on one of the rotating shaft member and the guide housing,
Wherein a guide groove corresponding to the guide member and to which the guide member is movably coupled is formed on the other of the rotation axis member and the guide housing. The method according to claim 1,
Wherein the rupture unit further comprises a mixing member formed on the rotating shaft member to promote mixing of the first sample and the second sample,
Wherein the mixing member is spaced apart from the rupturing member. The method according to claim 1,
Wherein the guide member is protruded outside the rotating shaft member and has a guide protrusion inserted into the guide groove and moving along the guide groove. The method according to claim 1,
And the guide housing is disposed on an upper side of the second multi-well plate so as to communicate with the second well. The method of claim 3,
Both end portions of the guide groove are spaced apart from each other by a predetermined angle,
And the rotating shaft member is moved in the vertical direction while being rotated by the predetermined angle. 6. The method of claim 5,
Wherein the guide groove is obliquely formed with respect to the longitudinal direction of the guide housing. The method according to claim 1,
Wherein the rupture portion further comprises an elastic member disposed inside the guide housing,
Wherein the elastic member provides an elastic force to the rotating shaft member such that the rotating shaft member is repeatedly moved in the vertical direction. 8. The method of claim 7,
Further comprising a support plate disposed between the guide housing and the second multi-well plate,
Wherein the support plate is formed with a support hole through which the rotation axis member is inserted. 9. The method of claim 8,
Wherein the elastic member is disposed between the guide member and the support plate and is supported by the support plate when the rotation axis member and the guide member are moved downward. The method according to claim 1,
And the second multi-well plate is disposed on top of the first multi-well plate such that each of the second wells is at least partially inserted into each of the first wells. The method according to claim 1,
Wherein the first multiwell plate includes a first connecting member for interconnecting the plurality of first wells,
Wherein the second multi-well plate includes a second connecting member for interconnecting the plurality of second wells,
Wherein the second multi-well plate is supported on the first multi-well plate by supporting the second connection member above the first connection member. The method according to claim 1,
Wherein the plurality of first wells are arranged in a lateral direction and a longitudinal direction on the same plane,
Wherein the plurality of second wells are arranged in a lateral direction and a longitudinal direction on the same plane. 13. The method of claim 12,
Wherein the rupturing portion comprises a plurality of rupturing portions corresponding to the number of the second wells,
Wherein each of the rupture parts is arranged in parallel in the transverse and longitudinal directions on the upper side of the second multi-well plate. 14. The method of claim 13,
Further comprising a driving portion for pressing the rotating shaft member of the rupturing portion toward the lower side,
Wherein the driving unit comprises a plurality of rupturing members so as to correspond to the number of the rupturing members arranged in a row in a row in the lateral direction. 15. The method of claim 14,
Further comprising: a first multi-well plate, a second multi-well plate, a moving unit moving the rupturing unit in the longitudinal direction, and a control unit controlling the moving unit,
The control unit may be configured such that, when all of the separation membranes of the plurality of second wells constituting a column of the second multiwell plate are ruptured, the moving unit moves the first multiwell plate, And a rupturing portion is moved to a neighboring column.

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