KR20180082404A - Novle Rhodobacter sphaeroides KNU-04 strain having plant growth promoting effect, and uses thereof - Google Patents

Abstract

The present invention relates to a rhodobacter sphaeroides KNU-04 strain having plant growth promoting and nitrogen fixing activity, and a microbial preparation using the same. The novel strain of the present invention is experimentally confirmed to have plant growth promoting and nitrogen fixing ability, thereby being usefully used for environmentally friendly farming.

Description

A novel Rhodobacter sphaeroides KNU-04 strain having plant growth promoting effect, and uses thereof {Novle Rhodobacter sphaeroides KNU-04 strain having plant growth promoting effect, and uses thereof}

The present invention relates to a novel Rhodobacter sphaeroides KNU-04 strain (accession number KCTC 13065BP) having plant growth promoting activity, a culture thereof, and a microbial preparation composition for promoting plant growth containing them as an active ingredient. About.

The most important elements for plant growth are nitrogen, phosphoric acid, and potassium. These elements play an important role in the overall growth of plants, so they are known as macroelements necessary for the growth of plants, and nitrogen fertilizers are widely used in the cultivation of crops. However, due to excessive fertilization of nitrogen fertilizers, nutrient accumulation of soil occurs, which causes yellowing of plants or overgrowth, which rather interferes with the growth of plants.

Recently, in order to solve this problem, research on microorganisms having nitrogen circulation capability is in progress, and photosynthetic bacteria are representative of this. Photosynthetic bacteria refers to microorganisms that use light to assimilate carbon. Among these photosynthetic bacteria, there are photosynthetic bacteria that have the ability to fix nitrogen, and they exist in the rhizosphere and are known to play a beneficial role in plant growth such as promoting crop growth, fertilizing soil, and improving continuous cropping disorders.

Recently, problems such as safety discomfort, environmental pollution, and persistence of chemical pesticides have become a hot topic, so many studies on eco-friendly farming methods are underway, and development of eco-friendly farming methods using useful agricultural microorganisms is also underway. An eco-friendly agricultural product quality certification system, classified according to the amount of chemical pesticides and fertilizers applied, was introduced, and it is known that farmers implementing this system actually cultivate crops in an eco-friendly way by reducing fertilization of chemical pesticides and nitrogen. Consumers also have a growing interest in well-being, and the demand for agricultural products that have been certified as eco-friendly agricultural products is steadily increasing.

Currently commercially available agricultural useful microorganisms include microbial preparations that have an antagonistic effect on plant pathogenic microorganisms, solubilization of inorganic nutrients in the soil, and promotion of various plant growth, and the number of chemical pesticides and fertilizers currently available is also decreasing. Therefore, a microbial agent that can replace it is also needed. In a related study, the Enterobacter rudwijii SJR3 strain that promotes plant growth has been proposed.

Republic of Korea Patent Publication No. 2015-0105546

As a result of the present inventors trying to develop an eco-friendly microbial preparation for promoting plant growth, the novel Rhodobacter sp. sphaeroides ) By confirming that the KNU-04 strain has plant growth promoting activity, the present invention was completed.

Accordingly, an object of the present invention is to provide a Rhodobacter sphaeroides KNU-04 strain (accession number KCTC 13065BP) having plant growth promoting activity and a microbial preparation using the same.

However, the technical problem to be achieved by the present invention is not limited to the problems mentioned above, and other problems that are not mentioned will be clearly understood by those skilled in the art from the following description.

In order to achieve the object of the present invention as described above, the present invention is Rhodobacter speroidis having a plant growth promoting activity (Rhodobacter sphaeroides ) KNU-04 strain (accession number KCTC 13065BP) is provided.

In one embodiment of the present invention, the strain is characterized in that it comprises the 16s rRNA nucleotide sequence of SEQ ID NO: 1.

In another embodiment of the present invention, the strain is characterized in that it comprises a biological growth hormone function gene having a plant growth promoting ability of SEQ ID NO: 2 to SEQ ID NO: 5.

In another embodiment of the present invention, the strain is characterized in that it contains a nitrogen-fixing function gene having a nitric acid-fixing ability of SEQ ID NO: 6 to SEQ ID NO: 15.

In addition, the present invention is the Rhodobacter speroidis (Rhodobacter sphaeroides ) KNU-04 strain (accession number KCTC 13065BP), a culture of the strain, a concentrate of the culture, a dried product of the culture and a combination thereof, comprising as an active ingredient at least one selected from the group consisting of, plant growth It provides a microbial preparation for promotion.

In addition, the present invention provides a microbial fertilizer for promoting plant growth, comprising the microbial preparation.

In addition, the present invention provides a method for promoting plant growth, comprising the step of treating a microbial agent in soil, plants, or seeds of plants.

Rhodobacter speroidis of the present invention (Rhodobacter sphaeroides ) KNU-04 strain was experimentally confirmed to have plant growth promoting activity, so it can be usefully used as an eco-friendly agricultural method microbial preparation, and by identifying gene sequences related to plant growth promotion and nitrogen fixation ability, it is effectively used under various conditions. Can be used.

1 is a Rhodobacter speroidis of the present invention (Rhodobacter sphaeroides ) is a graph showing the amount of auxin production of KNU-04 strain.
Figure 2 is the Rhodobacter speroids of the present invention (Rhodobacter sphaeroides ) is a diagram showing the nitrogen-fixing ability activity produced by the KNU-04 strain.
Figure 3 is the Rhodobacter speroids of the present invention (Rhodobacter sphaeroides ) The morphological characteristics of the KNU-04 strain were shown using Gram staining, and a micrograph of the strain was shown.
Figure 4 is a Rhodobacter speroidis of the present invention (Rhodobacter sphaeroides ) is a diagram showing the nucleotide sequence of the gene (rDNA) encoding the 16S rRNA of the KNU-04 strain.
Figure 5 is a Rhodobacter speroidis of the present invention (Rhodobacter sphaeroides ) is a diagram showing a phylogenetic schematic diagram comparing the nucleotide sequence of the gene (rDNA) encoding the 16S rRNA of the KNU-04 strain with a known strain.
Figure 6 is the Rhodobacter speroids of the present invention (Rhodobacter sphaeroides ) Tryptophan synthase alpha chain (EC 4.2.1.20; SEQ ID NO: 2), Tryptophan synthase beta chain (EC 4.2.1.20; SEQ ID NO: 3), Anthranilate phosphoribosyltransferase (EC 4.2.1.20; SEQ ID NO: 3), which shows the role of plant hormones related to plant growth promoting ability of KNU-04 strain EC 2.4.2.18; SEQ ID NO: 4), Phosphoribosylanthranilate isomerase (EC 5.3.1.24; SEQ ID NO: 5).
7 is a Rhodobacter speroidis of the present invention (Rhodobacter sphaeroides ) KNU-04 strain Nitrogenase (molybdenum-iron) alpha chain (EC 1.18.6.1; SEQ ID NO: 6), Nitrogenase (molybdenum-iron) beta chain (EC 1.18.6.1; SEQ ID NO: 7) showing a role for nitrogen fixation ability ), Nitrogenase (molybdenum-iron)-specific transcriptional regulator NifA (SEQ ID NO: 8), Nitrogenase FeMo-cofactor synthesis FeS core scaffold and assembly protein NifB (SEQ ID NO: 9), Nitrogenase FeMo-cofactor scaffold and assembly protein NifE (SEQ ID NO: 10) ), Nitrogenase (molybdenum-iron) reductase and maturation protein NifH (SEQ ID NO: 11), Nitrogenase FeMo-cofactor scaffold and assembly protein NifN (SEQ ID NO: 12), Nitrogenase FeMo-cofactor synthesis molybdenum delivery protein NifQ (SEQ ID NO: 13), Nitrogenase This is the gene base sequence of the stabilizing/protective protein NifW (SEQ ID NO: 14) and Nitrogenase FeMo-cofactor carrier protein NifX (SEQ ID NO: 15).

The present invention provides a Rhodobacter sphaeroides KNU-04 strain (accession number KCTC 13065BP) having plant growth promoting activity.

The sequence of the gene (rDNA) encoding the 16S rRNA of the strain is represented by SEQ ID NO: 1.

The nucleotide sequence of SEQ ID NO: 1 is Rhodobacter, a publicly known strain. sphaeroides ATH 2.4.1 ^T and 99% homology, 1% difference between the two strains was recognized, it was found to be a new strain.

Accordingly, the present inventors named the obtained novel strain as Rhodobacter sphaeroides KNU-04 strain, and deposited with the Korea Research Institute of Bioscience and Biotechnology Biological Resource Center (KCTC) on August 19, 2016, and the accession number It was given KCTC 13065BP.

The strain of the present invention can be obtained by separating and identifying from soil samples, reducing nitric acid, using glucose as an energy source under anaerobic conditions, and using D-glucose, D-fructose, D-xylose, and horses. It was confirmed that carbon sources such as rate, lactate, and glycerol were used as energy sources.

The Rhodobacter speroidis (Rhodobacter sphaeroides ) KNU-04 strain has the ability to produce plant growth hormone and the ability to fix nitrogen.

In the present invention, plants to be targeted for growth promotion are preferred for rice crops such as wheat and barley, but are not limited thereto.

Plants have a system that copes with and adapts to various environmental changes by regulating growth and development. In particular, plant development regulation occurs mainly through the interaction between the external environment and the plant's intrinsic development program, and plant hormones are known to perform essential functions for such interaction. In particular, auxin (IAA) is a plant. It is known to germinate and grow from this seed, or to be involved in the elongation of the stem.

On the other hand, the plant growth promoting ability was confirmed through a measurement test of the production amount of auxin (IAA), which is a plant growth promoting hormone produced by the strain (Fig. 1), and the nitrogen fixation ability measured the nitrogen fixation ability produced by the strain. It was confirmed in the test (Fig. 2).

In addition, the morphological characteristics of the strain were confirmed by Gram staining method (FIG. 3), and the biochemical characteristics were confirmed in the Api kit 20 measurement test (Table 1).

Therefore, it was confirmed that the strain of the present invention has an effect of promoting plant growth, and accordingly, the Rhodobacter sp. sphaeroides ) The gene (rDNA) encoding the 16S rRNA of the KNU-04 strain was identified. The gene (rDNA) encoding the 16S rRNA of the strain was confirmed by 16S rRNA gene sequencing (SEQ ID NO: 1, Fig. 4).

The Rhodobacter speroidis (Rhodobacter sphaeroides ) The phylogenetic schematic diagram using a gene (rDNA) encoding 16S rRNA of the KNU-04 strain was confirmed and shown in FIG. 5. The phylogenetic schematic diagram of the strain was confirmed in Molecular Evolutionary Genetics Analysis 6.0 version.

In addition, in the present invention, the Rhodobacter sp. sphaeroides ) The results of confirming the genes related to plant growth promotion using the whole genome of the KNU-04 strain are shown in FIG. 6. The genes of the strain were identified by ion torrent PGM genome sequencing.

The Rhodobacter speroidis (Rhodobacter sphaeroides ) The results of confirming the genes related to nitrogen fixation ability using the whole genome of the KNU-04 strain are shown in FIG. 7. The genes of the strain were identified by ion torrent PGM genome sequencing.

In a specific embodiment of the present invention, the production amount of auxin, which is a plant growth promoting hormone, was measured and the activity of nitrogen fixation was confirmed, and Tryptophan synthase alpha chain (EC 4.2.1.20; SEQ ID NO: 2), which represents the role of plant growth hormone, and Tryptophan synthase The gene base sequence of beta chain (EC 4.2.1.20; SEQ ID NO: 3), Anthranilate phosphoribosyltransferase (EC 2.4.2.18; SEQ ID NO: 4), Phosphoribosylanthranilate isomerase (EC 5.3.1.24; SEQ ID NO: 5) plays a role in the ability to fix nitrogen. Nitrogenase (molybdenum-iron) alpha chain (EC 1.18.6.1; SEQ ID NO: 6), Nitrogenase (molybdenum-iron) beta chain (EC 1.18.6.1; SEQ ID NO: 7), Nitrogenase (molybdenum-iron)-specific transcriptional regulator NifA (SEQ ID NO: 8), Nitrogenase FeMo-cofactor synthesis FeS core scaffold and assembly protein NifB (SEQ ID NO: 9), Nitrogenase FeMo-cofactor scaffold and assembly protein NifE (SEQ ID NO: 10), Nitrogenase (molybdenum-iron) reductase and maturation protein NifH (SEQ ID NO: 11), Nitrogenase FeMo-cofactor scaffold and assembly protein NifN (SEQ ID NO: 12), Nitrogenase FeMo-cofactor synthesis molybdenum delivery protein NifQ (SEQ ID NO: 13), Nitrogenase stabilizing/protective protein NifW (SEQ ID NO: 14), the gene base sequence of the Nitrogenase FeMo-cofactor carrier protein NifX (SEQ ID NO: 15) was revealed.

Therefore, the present invention is Rhodobacter sp. sphaeroides ) KNU-04 strain, a culture of the strain, a concentrate of the culture, a dried product of the culture, and a microorganism preparation/fertilizer for promoting plant growth, comprising as an active ingredient at least one selected from the group consisting of It is possible to provide a method of promoting plant growth by treating the microbial agent/fertilizer.

In the present invention, the culture is preferably obtained by separating from a normal agar medium (or nutrient agar) medium, or a tryptic soy agar (TSA) medium in which the strain is cultured, but is not limited thereto.

The microbial preparation composition may be formulated for promoting plant growth by a conventional method, and may be prepared in the form of dry powder or liquid fertilizer. Specifically, the microbial preparation composition according to the present invention may be prepared in the form of a liquid fertilizer, and may be used in the form of powdered powder by adding an extender thereto, or may be formulated and granulated. However, it is not particularly limited to the formulation. Preferably, it can be formulated as a biofertilizer for promoting plant growth to replace the chemical fertilizer, that is, it can be formulated as a biofertilizer to overcome this in eco-friendly organic farming where the supply of chemical fertilizers is limited.

In the present invention, the microbial preparation can be prepared by adding additives such as additives, extenders, and nutrients to a strain or a culture thereof. At this time, additives include polycarboxylate, sodium lignosulfonate, calcium lignosulfonate, sodium dialkyl sulfosuccinate, sodium alkyl aryl sulfonate, polyoxyethylene alkyl phenyl ether, sodium tripolyphosphate, polyoxyethylene alkyl From the group consisting of aryl phosphoric esters, polyoxyethylene alkyl aryl ethers, polyoxyethylene alkyl aryl polymers, polyoxyalkylone alkyl phenyl ethers, polyoxyethylene nonyl phenyl ethers, sodium sulfonate naphthalene formaldehyde, Triton 100 and Tween 80 One or more selected can be used, and one or two or more selected from the group consisting of skim milk (medium), soy flour, rice, wheat, loess, diatomaceous earth, bentonite, dextrin, glucose, and starch as an extender and nutrient And, as the disintegrant, at least one selected from the group consisting of bentonite, talc, dialite, kaolin, and calcium carbonate may be used.

In addition, the present invention provides a method for promoting plant growth by treating the microbial agent to soil or plants. At this time, treatment methods are generally used, that is, spraying (e.g. spraying, misting, atomizing, powder spraying, granule spraying, water surface application, regular use, etc.), soil application (e.g. mixing, irrigation, etc.) , Surface use (for example, coating, smearing, coating, etc.), immersion, poisoning, smoking, etc. can be carried out. The amount of use can be appropriately determined depending on the formulation, damage situation, application method, application location, etc.

In the present invention, the effective amount of microorganisms contained in the preparation to be treated according to the above method may be included as the number of microorganisms of ^{1 to 1×10 100 per cultivated land area (m 2 ).} In addition, the effective amount of microorganisms contained in the formulation treated by spraying among the above methods ^{may be included in a concentration of microorganisms of 1 to 1×10 100} per ml, and the effective amount of microorganisms contained in the composition treated by immersion is 1 per ml. It may be included in a microbial concentration of 1×10 ^100.

Hereinafter, a preferred embodiment is presented to aid the understanding of the present invention. However, the following examples are provided for easier understanding of the present invention, and the contents of the present invention are not limited by the following examples.

[ Example ]

Example One. Rhodobacter Spareroids ( Rhodobacter sphaeroides ) KNU -04 Isolation and identification of strains

1-1. From the soil Rhodobacter Spareroids ( Rhodobacter sphaeroides ) KNU -04 Isolation and identification of strains

Soil samples were collected from the root area of a melon field known to be of good quality in Sangju City, Gyeongsangbuk-do to separate microorganisms that can be used as plant growth accelerators. For separation of microorganisms from the soil, 1 g of a soil sample was added to 9 mL of 0.85% NaCl solution, stirred sufficiently, and diluted stepwise using 9 mL of new 0.85% NaCl solution. 100 μL was taken out from each diluted sample section, and then spread on nutrient agar. Microorganisms grown as single colonies were separated using a morphological classification method using the appearance of the colony, and the final isolate strain was selected by evaluating the activity as a plant growth promoter using the isolated microorganism. The final isolated strain was identified by amplifying the 16S rRNA gene region and analyzing the base sequence.

1-2. Measurement of plant growth promoting hormone production

To confirm the production of auxin, the isolated strain was inoculated into 5 mL of nutrient broth medium containing 3 mg/mL of L-Tryptophan, and then cultured for 16 hours under the same culture conditions. Of these, 1 mL was collected, centrifuged at 12,000 rpm for 5 minutes, and the supernatant was filtered through a 0.20 μm membrane filter to prepare a culture supernatant. 500 μL of the culture supernatant was transferred to a test tube, mixed with 1 mL of salkowski regent, and reacted under dark conditions for 30 minutes. After the reaction, the absorbance was measured at 535 nm, and the quantification of auxin was calculated by substituting it into a calibration curve prepared using an IAA standard (Sigma Aldrich, Germany).

As a result, as shown in FIG. 1, it was confirmed that the amount of auxin production of the KNU-04 strain, which is the final isolated strain, is significantly higher than that of other isolated strains.

1-3. Nitrogen fixation ability measurement

To confirm the nitrogen fixation ability, the isolated strain was streaked on nutrient agar medium and cultured under the same culture conditions to obtain a single colony. The isolated single colony was collected with nitrogen fixation agar (Sucrose 6 g, MgSO ₄ 7H ₂ O 0.2 g, Na ₂ HPO ₄ 13.9 g, KH ₂ PO4 1.7 g, NaCl 2 g, FeCl ₃ 6H ₂ O 8 mg, Na ₂ MoO ₄ 2H ₂ O 3 mg, Thiamin 1 mg, Agar 15 g) were used with sterilized toothpicks or streaked smeared, and nitrogen fixation ability was confirmed based on growth.

As a result, as shown in FIG. 2, it was confirmed that the KNU-04 strain, which is the final isolated strain, was grown while growing in a medium using nitrogen in the atmosphere as a nitrogen source, and it was confirmed that it has nitrogen fixing ability.

1-4. Identification of isolated strains

After extracting genomic DNA from the recovered cells (Thompson, 1980) for identification of strains with excellent plant growth promoting activity and nitrogen fixation ability, the polymerase chain was used as a template using the isolated genomic DNA. reaction; PCR) to amplify the 16S rRNA gene. The universal primer used at this time was 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3'), respectively (Lane 1991). The amplified PCR product was purified using a PCR purification system (Solgent, Daejeon, Korea). Sequencing to analyze the entire nucleotide sequence of the purified PCR product was performed by requesting Solgent (Solgent, Daejeon, Korea).

The analyzed nucleotide sequence results were compared using BLASTN of NCBI, and the homology and phylogenetic tree of nucleotide sequences were analyzed using neighbor-joining methods through Bioedit and Mega6 programs. . The results are shown in FIGS. 4 and 5.

The isolated strain is the previously reported strainRhodobacter sphaeroides ATH 2.4.1^TIt was confirmed to have a homology of 99%. The strain is a conventionalRhodobacter sphaeroides ATH 2.4.1^TSince it differs from 1%, it is recognized as a new strain belonging to the sp. sp. sp., and it is Rhodobacter sp. sp.Rhodobacter sphaeroides) Named KNU-04, and the strain was deposited with the Korea Research Institute of Bioscience and Biotechnology Biological Resource Center (accession number KCTC13065BP). In addition, as shown in Fig. 4, the present Rhodobacter sp.Rhodobacter sphaeroides) The nucleotide sequence of the gene (rDNA) encoding the 16S rRNA of the KNU-04 strain was confirmed.

Example 2. Rhodobacter Spareroids ( Rhodobacter sphaeroides ) KNU -04 Morphological and biochemical characteristics of strain

2-1. Morphological characteristics

Rhodobacter sp. sphaeroides ) Gram staining was performed to confirm the morphological characteristics of the KNU-04 strain. After culturing the KNU-04 strain in nutrient agar, which is a complete medium, using stroke smearing, a single colony was recovered. The recovered single colonies were suspended in 0.85% NaCl solution, placed on a slide glass, and thermally fixed using an alcohol lamp, followed by crystal violet staining, iodine staining, ethanol bleaching, safranin staining, and finally distilled water. After washing the dye with an optical microscope, the morphological appearance of the KNU-04 strain was confirmed. As a result, Rhodobacter sp. sphaeroides ) KNU-04 strain was confirmed to be a Gram-negative bacteria, and it is shown in FIG. 3.

2-2. Check sugar metabolism characteristics

Rhodobacter sp. sphaeroides ) The sugar metabolism ability of the KNU-04 strain was tested according to the supplier's experimental method using an API 20 NE kit (Bio Merioux), and was incubated for 48 hours in a 30°C incubator and then compared. The results were prepared using the color change of the API 20 NE kit and the turbidity according to the growth of the strain, and the results are shown in Table 1.

Active ingredient result Active ingredient result D-glucose + Malate + D-Fructose + Lactate + D-lactose - Citrate - D-Sucrose - Glycerol + D-maltose - D-mannitol - D-xylose + D-sorbitol -

As shown in Table 1 above, the Rhodobacter sphaeroides KNU-04 strain of the present invention uses D-glucose, D-fructose, D-xylose, malate, lactate, and glycerol as an energy source. It was confirmed to be used.

Example 3. Rhodobacter Spareroids ( Rhodobacter sphaeroides ) KNU -04 Promote plant growth of strains, phosphoric acid Solubilization Ability-related gene sequence information

The present inventors described Rhodobacter speroidis that can be used as a plant growth promoter in Example 1 above. sphaeroides ) In order to reveal the gene sequence related to plant growth promotion and nitrogen fixation ability of KNU-04 strain, after extracting genomic DNA from the cells, using Ion torrent PGM sequencing equipment, Rhodobacter sphaeroides sphaeroides ) The entire gene sequence of the KNU-04 strain was revealed.

For gene sequence information related to plant growth promotion and nitrogen fixation ability, the entire gene sequence information of the KNU-04 strain was uploaded to the RAST server, and gene sequence analysis was performed, and the gene sequence related to the corresponding ability was confirmed.

Tryptophan synthase alpha chain (EC 4.2.1.20), Tryptophan synthase beta chain (EC 4.2.1.20), Anthranilate phosphoribosyltransferase (EC 2.4.2.18), Phosphoribosylanthranilate isomerase (EC 5.3.1.24), which represent the gene sequences of enzymes related to plant growth promoting ability. ) Is shown in FIG. 6.

In addition, Nitrogenase (molybdenum-iron) alpha chain (EC 1.18.6.1), Nitrogenase (molybdenum-iron) beta chain (EC 1.18.6.1), Nitrogenase (molybdenum-iron)-specific transcriptional regulator NifA, which show a role for nitrogen fixation ability. , Nitrogenase FeMo-cofactor synthesis FeS core scaffold and assembly protein NifB, Nitrogenase FeMo-cofactor scaffold and assembly protein NifE, Nitrogenase (molybdenum-iron) reductase and maturation protein NifH, Nitrogenase FeMo-cofactor scaffold and assembly protein NifN, Nitrogenase FeMo-cofactor The gene base sequences of the synthesis molybdenum delivery protein NifQ, Nitrogenase stabilizing/protective protein NifW, and Nitrogenase FeMo-cofactor carrier protein NifX are shown in FIG. 7.

The above description of the present invention is for illustrative purposes only, and those of ordinary skill in the art to which the present invention pertains will be able to understand that other specific forms can be easily modified without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and are not limiting.

Korea Research Institute of Bioscience and Biotechnology KCTC13065BP 20160819

<110> Kyungpook National University Industry-Academic Cooperation Foundation <120> Novle Rhodobacter sphaeroides KNU-04 strain having plant growth promoting effect, and uses thereof <130> MP16-305_division <160> 15 <170> KoPatentIn 3.0 <210> 1 <211> 1271 <212> RNA <213> Rhodobacter sphaeroides KNU-04 16S rRNA <400> 1 ggacgggtga gtaacgcgtg ggaacgtgcc ctttgcttcg gaatagcccc gggaaactgg 60 gagtaatacc gaatgtgccc tacgggggaa agatttatcg gcaaaggatc ggcccgcgtt 120 ggattaggta gttggtgggg taatggccta ccaagccgac gatccatagc tggtttgaga 180 ggatgatcag ccacactggg actgagacac ggcccagact cctacgggag gcagcagtgg 240 ggaatcttag acaatgggcg caagcctgat ctagccatgc cgcgtgatcg atgaaggcct 300 tagggttgta aagatctttc aggtgggaag ataatgacgg taccaccaga agaagccccg 360 gctaactccc gtgccagcag ccgcggtaat acggaggggg ctagcgttat tcggaattac 420 tgggcgtaaa gcgcacgtag gcggatcgga aagtcagagg tgaaatccca gggctcaacc 480 ctggaactgc ctttgaaact cccgatcttg aggtcgagag aggtgagtgg aattccgagt 540 gtagaggtga aattcgtaga tattcggagg aacaccagtg gcgaaggcgg ctcactggct 600 cgatactgac gctgaggtgc gaaagcgtgg ggagcaaaca ggattagata ccctggtagt 660 ccacgccgta aacgatgaat gccagtcgtc gggcagcatg ctgttcggtg acacacctaa 720 cggattaagc attccgcctg gggagtacgg ccgcaaggtt aaaactcaaa ggaattgacg 780 ggggcccgca caagcggtgg agcatgtggt ttaattcgaa gcaacgcgca gaaccttacc 840 aacccttgac atggcgatcg cggttccaga gatggttcct tcagttcggc tggatcgcac 900 acaggtgctg catggctgtc gtcagctcgt gtcgtgagat gttcggttaa gtccggcaac 960 gagcgcaacc cacgtcctta gttgccagca ttcagttggg cactctaggg aaactgccgg 1020 tgataagccg gaggaaggtg tggatgacgt caagtcctca tggcccttac gggttgggct 1080 acacacgtgc tacaatggca gtgacaatgg gttaatccca aaaagctgtc tcagttcgga 1140 ttggggtctg caactcgacc ccatgaagtc ggaatcgcta gtaatcgcgt aacagcatga 1200 cgcggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccatgg gaattggttc 1260 tacccgaagg c 1271 <210> 2 <211> 792 <212> DNA <213> Tryptophan synthase alpha chain (EC 4.2.1.20) <400> 2 atgacgcgga tcgacgacac cttccggcgg cttcgggccg aggggaagaa ggccttcgtg 60 gcctatatca tggcgggcga tcccgatctc gagacgtcgc ttgcggtgat gcggggcctg 120 ccggaggcgg gcgtggacat catcgagctg ggcatgccct tcaccgatcc gatggccgac 180 ggcccgacga tccagacggc cggccagcgc gccctcgagg gcggccagac gctgacccgg 240 acgctcgaga tggtgcgcgc cttccgggcg gagaatgccg agacgcccat cgtgatgatg 300 ggttactaca atccgatcta tgcgcgcggc gtcgagacct tcctcgccga ggcgacggag 360 gccgggatcg atgggctgat cgtggtggac ctgccgcccg aggaggatgc cgagctctgc 420 ctgcccgcac aggcggcggg gctgaacttc atccggctcg cgacccccac caccgacagc 480 cgccgcctgc ccaaggtgct gcagaacacc tcgggcttcg tctattacgt ctcgatcacc 540 ggcatcaccg gcgcggcggc cgcgcaggcg gccgatgtgg cgcccgaagt ggcgcgcatc 600 aaggccgcga cggatctgcc ggtcatcgtg ggcttcggca tcaccacccc cgaggccgcg 660 caggacctcg cgggtatcgc cgacggctgc gtggtgggct cggcgatcgt gaagctcgtg 720 ggcgagggcc ggccggtggc cgaggtgctg gaccgggtgg cggcgctcgc ggccggcgct 780 cacgcggcct ga 792 <210> 3 <211> 1230 <212> DNA <213> Tryptophan synthase beta chain (EC 4.2.1.20) <400> 3 atggccgagg acggcatcaa cagctacatg accggccccg acgagcaggg tcgtttcggc 60 atcttcggcg gacgcttcgt gtccgagacg ctgatgccgc tgattctcga tctcgaagcg 120 cgctatgaac atgccaagac cgacccggat ttctgggccg agatggacga cctgtggaag 180 aactacgtcg gtcgtccctc gccgctctat ttcgcgccgc ggctgaccga gcatctcggc 240 ggggcgaaga tctatctcaa gcgcgacgag ctgaaccata ccggcgcgca caagatcaac 300 aatgtgctgg gccagatcat cctcgcccgc cgcatgggca agacccggat catcgccgag 360 acgggcgcgg gccagcatgg cgtggcgacg gcgacggtct gcgccaagtt cgggctgaaa 420 tgcgtggtct acatgggcgc gcacgatgtc gaacggcagg cgccgaacgt gttccgcatg 480 cggctgctgg gcgccgaggt ggtgccggtg acctccgggc gcggcacgtt gaaggatgcg 540 atgaacgacg cgctgcgcga ctgggtcacc aacgtgcgcg acaccttcta ctgcatcggc 600 accgtggcgg gcccgcatcc ctatccggcg atggtgcgcg acttccagtc gatcatcggc 660 cgcgaagtgc gctggcagct ggccgagcag gaagagggcc gcctgcccga cacgctcgtg 720 gccgccatcg gcggcgggtc gaacgccatg gggctgttcc atccgttcct cgacgatccg 780 tcggtgcgga tcgtcggcgt cgaggccgga ggcaagggcg tcgacgaccg gatggaacat 840 tgtgcctcgc tgaccggcgg acgtccgggc gtgctgcacg gcaaccggac ctatctgctg 900 caggatgccg acggccagat cctcgagggc ttctcgatct cggccgggct cgactatccg 960 ggcatcgggc ccgaacatgc atggctccat gacacgggcc gggcggaata tgtctcgatt 1020 accgatgccg aggcgctgga ggcgttccag ctctgctgtg cgctcgaagg gatcattccc 1080 gcgctcgagc cgtcgcacgc gctggcccat gtcatcaaga tcgcgccgac gctgccgcgc 1140 gaccatatca tcgtgatgaa catgtgcggg cggggcgaca aggacatctt caccgtggcg 1200 aagcatctgg gcttcgacat gaagatctga 1230 <210> 4 <211> 1017 <212> DNA <213> Anthranilate phosphoribosyltransferase (EC 2.4.2.18) <400> 4 atgagcgacc ggctgaagcc cctgatcggc accgcggcca cccgccccct cagccgcgag 60 gaggccgagt tcgccttcga gtgcctgttc gagggcgagg ccacgcccgc gcagatgggg 120 ggcctgctga tggcgctgcg gacccgcggc gagacggtgg acgaatatgc cgccgccgcc 180 tcggtcatgc gggccaagtg ccacaaggtg cgcgccccgc acggcgccat cgacatcgtg 240 ggcaccgggg gcgacggcaa gggcacgctg aacatctcga ccgccacggc cttcgtggtg 300 gcgggggcgg gcgtgccggt cgccaagcac ggcaaccgca acctctcgtc gaagtccggc 360 gccgccgatg cgcttaccga gatgggcctc aatgtcatga tcggccccga acaggtcgag 420 gcctgcctgc tggaggccgg gatcggcttc atgatggcac cgatgcacca tccggccatg 480 cgccatgtcg ggccggtgcg ggccgagctc gggacgcgga cgatcttcaa catcctcggg 540 ccgctgacca atccggcggg ggtgaagcgc cagctgaccg gcgccttctc gcccgacctc 600 atccggccga tggccgaggt gctctccgcg ctcggctccg agaaggcatg gctcgtccat 660 ggcggcgacg ggacggacga gctcgcgatc tcggccgcct cgaaggtcgc ggcgctcgag 720 ggcgggcaga tccgcgaatt cgaactgcat cccgaggagg cgggtctgcc cgtccatccg 780 ttcgaggaga tcgtgggcgg cacacccgcc gagaatgcgc aggccttccg cgcgctgctc 840 gacggcgcgc cgggcgccta ccgcgatgcg gtgctgctga atgcggcggc ggcgctcgtg 900 gtggccgacc gcgcggcgca tctgcgcgaa ggggtggaga tcgccaccga cagcatcctg 960 tccggtgccg ccaaggcgaa ggtcgccctg ctggcccggc tgacgaacgc cgcctga 1017 <210> 5 <211> 591 <212> DNA <213> Phosphoribosylanthranilate isomerase (EC 5.3.1.24) <400> 5 gtgaaggcgg ccgcgtcctc gggcgcggcc tatgtcggtc tggtgttctt cccgaaatcg 60 ccgcgccatc tggagcttgc gcaggcgcag cggctcgcgc tcgccgcgcc gccgggggtg 120 gccaaggtgg cgctgacggt cgacgccagt gacgagacgc tcgacgccat cgtcgaggcg 180 gtgccgctcg acatgctgca gcttcacggc ggcgagagcc ccgagcgggt ggccgaggtg 240 cgggcgcgct acgggctgcc ggtcatgaag gccgtggggg tggcggacga aggcgacctg 300 ccgcagattc tcgaacagtc gctggccgcc gaccagatcc tgatcgatgc aaaaccgccg 360 aagggcgcgg cgctgcccgg cggcaatggc ctctccttcg actggcggct gatctcgggg 420 cggcactgga tcaggccctg gatgctggcg ggggggctga cggtcgaaaa tctggccgag 480 gccgtccggc gcacgggggc gtcgcaggtc gatgtctcct cgggcgtcga atcggcgccg 540 ggcgtcaagg atccggcccg gatcgcggcc ttcctgcagg ccgcccggtg a 591 <210> 6 <211> 1482 <212> DNA <213> Nitrogenase (molybdenum-iron) alpha chain (EC 1.18.6.1) <400> 6 atggcgaaag atatcgctga ctctgccgag accaacatga agctgatcga ggaggtgctg 60 gccgcctacc ccgacaaggc caggaagaag cgcgccaagc acctgaatgt cgcagcgccc 120 gtcgccgagg ccgaacccgg cctccagtcg aaatgcgaca atgtgaaatc gaacatcaag 180 tcggtccccg gcgtgatgac catccgcggc tgcgcctatg ccggctcgaa gggcgtggtc 240 tggggcccgg tcaaggacat gctgcacatc agccacggcc cggtcggctg cggccactac 300 agctggtccc agcgccgcaa ctactacacc ggcacgacgg gcgtggattc gttcgtgacc 360 atgcaggtca ccaccgactt ccaggaaaac gacatcgtct tcggcggtga caagaagctg 420 gaaaagacca tcgacgagct gaacatgctc ttcccgctga acaaggggat ctcgatccag 480 tcggaatgcc cgatcggcct gatcggcgac gacatcgagg cggtgtcgaa gaagaaggcc 540 aaggacatcg gcaagcgcgt cgttccggtg cgctgcgagg gcttccgcgg cgtgtcgcag 600 tcgctcggcc accatatcgc gaacgacatg atccgcgact gggtgctgga agcgggcgag 660 ggcgcgcgcg cgggctacga gcccggcccc tatgacgtga acatcatcgg cgactacaac 720 atcggcggcg acgcctggtc gagccggatc ctgctggaag agatcggcct caacgtcatc 780 gcgcaatggt cgggcgacgc caccatcgcc gagatggagc gcgctccggc ggcgaagctg 840 aacctcatcc actgctaccg ttcgatgagc tacatctgcc ggcacatgga agagaaccac 900 ggcgtgccgt ggatggagta caacttcttc ggcccctcgc agatcgcggc ctcgctgcgc 960 gccatcgccg cgaagttcga cgacaggatc caggccaatg ccgaagcggt catcgcgaaa 1020 taccagccgc tcgtcgatgc ggtgaacgcg aaatacaagc cgcgcctcga aggcaagaag 1080 gtgatgctct atgtgggcgg cctgcgtccg cgccacgtcg tcgacgccta ccatgacctg 1140 ggcatggaga tcgtgggcac cggctacgaa ttcgcccaca acgacgacta caagcgcacc 1200 ggccattaca tcaaggaagg cacgctgatc ttcgacgacg tctcgggcta cgagctggag 1260 aaattcgtcg aggcgatccg tcccgatctc gtgggctcgg gcatcaagga gaaatacaac 1320 acgcagaaga tgggcatccc gttccgtcag atgcactcct gggattattc cggcccctac 1380 cacggctacg acggctacgc gatcttcgcg cgcgacatgg atctcgcgat caacaacccc 1440 gtctggggca tgttcgatgc gccctggaag aagacggcct ga 1482 <210> 7 <211> 1521 <212> DNA <213> Nitrogenase (molybdenum-iron) beta chain (EC 1.18.6.1) <400> 7 atgccgcagt cggccgaaaa ggttctggat cacaaggatc tgttcaagga acccgaatat 60 caggcgatgc tcgagaagaa gcgcgccacc tacgagaatg cgacgcccgc cgagacggtg 120 gccgaaaccg cggactggac gaagtcctgg gactatcgcg agaagaacct cgcccgctcc 180 tgcgtgacca tcaacccggc caaggcctgc cagccgctcg gcgcggtctt cgccgccgcc 240 ggctatgaca gcaccatgag cttcgtgcac ggctcgcagg gctgcgtggc ctactatcgc 300 tcgcacctcg cccgccactt caaggagccg tcctcggcgg tgtcctcctc gatgaccgag 360 gatgcggcgg tgttcggcgg cctgaacaac atggtggaag gcctcgccaa cacctatgcg 420 ctctattcgc cgaagatgat cgccgtttcc accacctgca tggcggaagt catcggcgac 480 gacctcaact cgttcatcat caagtcgaag gagaaggaaa gcgtcccggc cgattttccg 540 gtgcccttcg cccatacgcc ggccttcgtg ggcagccacg tcgacggcta cgacaacatg 600 cagaagggca tcctgtcgaa cttctggaag gacgcgccgc gcaccgcggg cgaaggcctg 660 aacatcatcc cgggctttga cggctactgc gtgggcaacg tccgcgagat gaagcgcatg 720 ctcggcctga tgggcgtcga ggcgaccgtt ctgggcgatg cctcggacgt ctacgacacc 780 ccctcggacg gcgagtaccg catgtatgcg ggcggcacca cgcaggagga gatcaaggag 840 gccctgaacg cgaaggccac cctctcgctg caggaatatt gcacccgcag gacgctcgcc 900 ttctgcgagg aagtgggcca ggagaccgcg tcgttccact atccgatggg cgtcaaggcc 960 accgacgagt tcctgatgaa ggtctcggac ctgaccggca aggagatccc ggaagcgctc 1020 cgcctcgagc gcggccgcct gatcgacgcc atggccgaca gccaggccta cctgcacggc 1080 aagacctacg ccatcttcgg cgacccggac ttcgtctatg ccatggcccg cttcgtcatg 1140 gagatgggcg gcgagccgaa gcactgcctc gccaccaacg gcggcaagga ctgggaagtg 1200 cagatgaagg agctgctggc ctcctcgccc ttcggcgaag gctgccaggt ctgggcgggc 1260 aaggacctct ggcacctgcg ctcgatcctc gccacggaac cggcggacct gctgatcggc 1320 agcagctatg gcaagtatct cgagcgcgac tgcaacgtgc cgctgatccg cctgaccttc 1380 ccgatcttcg accgccacca ccaccaccgc ttcccgacct tcggctatca gggcgcgatc 1440 caggtgctgg tgaagatcct cgacaagatc ttcgacaagc tcgacgacga gtccgacatc 1500 tcgttcgacc tgacccgctg a 1521 <210> 8 <211> 1746 <212> DNA <213> Nitrogenase (molybdenum-iron)-specific transcriptional regulator NifA <400> 8 atggacacgt ccgcggcacg gtccggcgca gtggccgaac ggggtgagga atatctgacc 60 ctcgacgcgc tctgcgagat cgccaagctt ctgaccggcg cgagcgatcc gatcgcctgc 120 atgcctgcag tgttcggggt gctgggcgcc ttcatgggtc tgcggcacgg cgcgctcgcc 180 ctcctgcagg agggggcgca ggccgagacc cagcgcaatg cccgccacgt caatccctat 240 gtgatcgcgg ccaccgcctc gggcgtgccg cccgccgggg ccgaggcccg cgcgatcccc 300 gcgcaggtgg cgcggcatgt cttccgcaac ggcgtgtcgc tcgtttcctg cgacatcctc 360 gaggagttcg gcgccgaggc gctgccgccg ggcctcggcg acagccggca ggcgctggtg 420 gcggtgccga tccgcgatca ggccaattcg cccttcgtgc tgggcgtgct ctgcgcctac 480 cgcagcctca aggacaatgg cgcgcgctac ctcgacaccg acctgcgcgt cctgaacatg 540 gtggcggcgg tgctcgaaca gtcgatccgc ttccgccgtc tcgtcgcccg cgaccgcgac 600 cggatcgtgc aggaggcgcg cgaggcgatc cgggtcgcgg ccgaggcgac cgcgggcccg 660 cccgtcgagg cgccggccga ggcgctcgag ggggtgatcg gctcctcccc cgccatccag 720 cgcgtgatcg gccagatccg caaggtggcg ggcacccaca cgcccgtcct gctgcgcggc 780 gagagcggca ccggcaagga ggtcttcgct cgcgcgctcc atgcgctgtc cgagcggcgc 840 gacaaggcct tcatcaaggt caactgcgcg gcgctgagcc agtcgctgct cgaatccgaa 900 ctgttcggac atgagaaggg ctccttcacc ggcgccgtcc agcagaagaa gggccggttc 960 gagatggccg agggcggcac gctgtttctc gacgagatcg gcgaaatcag cctcgagttt 1020 caggccaagc tcctgcgcat cctgcaggag ggcgagttcg agcgggtggg gggcacgcgc 1080 acgctgcgcg tcgatgtccg gctggtgacg gccacgaaca aggatctcga gcgggcggtg 1140 gcgaacggca ccttccgcgc cgacctctat ttccgcatct gcgtggtgcc catcgtgctg 1200 ccgccgctgc gcgaccgcaa ggaggacatc ggccttctgg cgcaggggct gctcgagcgg 1260 ttcaacaagc gcaacgggat gaagaagaag ctgcatccct cggctgtggc cgcgcttgcc 1320 cagtgcaact tccccggcaa cgtgcgcgag ctcgagaact gcatcgcgcg tgtggcggcc 1380 ctctcgcccg agacggtgat ccacgccgac gatctggcct gccaccacga ccattgcctg 1440 tcggccgatc tctggcggct ccagaccgga tcggcctcgc cggtgggcgg gctcgcgcag 1500 gggccgctgg agctgccggt tctgggcagc cgcccgcccg cagccgcccc cagcgcgccg 1560 ccacccccgc cgccgaccgt cccctccgcg ccgctcgacg gcgaggcggc cgagcgcgag 1620 gcgctgatcg aggcgatgga gcgagccggc tgggtgcagg ccaaggccgc gcgcctgcgc 1680 ggcatgaccc cgcgccagat cggctatgcg ctgaagaaat acaacatccg ggtcgagaag 1740 ttctag 1746 <210> 9 <211> 1476 <212> DNA <213> Nitrogenase FeMo-cofactor synthesis FeS core scaffold and assembly protein NifB <400> 9 atggccaaca tcatctcgct gggcggacta caggtcgcgt cgcgcgacga gctcgggcag 60 gcgatggcgg gcggctgcac cgcttcctcc tgcgggacga aggcgggacc cgccgacatg 120 gacccggcga cctgggccaa ggttaaggat cacccctgct attccgaaga ggcgcaccat 180 tatttcgcgc ggatgcatgt ctcggtggcg cccgcctgca acatccagtg caactactgc 240 aaccgcaagt atgactgcgc caacgagagc cgccccggcg tcgtctccga gcggctgacg 300 cccgagcagg cggcgcgcaa ggtgctggcc gtggccgccg aggtgccgca gctgtcggtg 360 ctgggcatcg cgggcccggg cgatgcggcc tatgactgga agaagacgaa ggccaccttc 420 gacaaggtcc agtcgcagct gcccgacatc aagctctgcc tctcctccaa cgggctcgcc 480 atgcccgatc atgtcgagga gatcgtggcg atgaacatcg accatgtgac gctgaccatc 540 aacacgctcg atcccgaggt gggagcgaag atctatccgt gggtcttctt ccgcggcaag 600 cgacacgagg gggttgaggg ggcggcgatc ctcctcgcgc gccagatgga ggcgctcgac 660 atgctggtcg cccgcggggt gctggtgaag gtgaattcgg tcctgatccc cggcatcaac 720 gatgcgggca tggtcgagct gaaccgggag gtgaaggccc ggggcgcgtt cctgcacaac 780 atcatgccgc tgatctcgga tccggcgcat ggcacccatt tcggcctcac cggccagcgt 840 ggcccgacgg cggccgagct tcgggcggtg caggaccagt gcggcgacgg ggcgaacctg 900 atgaagcact gccgccagtg ccgcgccgat gcggtgggga tgctgggcga ggaccgcggg 960 caggagttca cgctcgacaa gctgcccgag acggtcgcgg aggatggcga ggagaagcgc 1020 gcagcctatc gcgactgggt ggcgcgcgag cgggccgacc ggcgcgccgc gaccgaggcc 1080 gcgcaggccg aggccgccgc cctcgccgcg ccgccgatgc gcgtggccgt ctgcaccaag 1140 ggcggcgggc gcatcaacca gcatttcggc catgccaccg agttccagat ctacgaggtg 1200 gatgcgggcg gtgtgcgctt cctcactcac cggcgggccg acaattactg cgtgggcggc 1260 cacggcgaga gcgaccggct gaccgagatc gtccgcacgc tcgacggtgt gccggtcgtg 1320 ctctgcgccc gcatcggcga ggggccgcgg tcccggatgg ccgaagctgg catcgaggtg 1380 atcgacgcct gcgccatgga ttacatcgag accgcgctcc tcgacctcta tgccgaccgc 1440 aaccgcagcc ccgacgcggg cgcgctcagc gcctga 1476 <210> 10 <211> 1464 <212> DNA <213> Nitrogenase FeMo-cofactor scaffold and assembly protein NifE <400> 10 atgtcggaag ccttgaagca gaagatccag gacgcctttc acgagccggg ctgcgccacg 60 aacaccgcca agtccgaggg cgagcgccgg aagggctgcg cgaaacagct gacgcccggc 120 gcggcggccg ggggctgcgc cttcgacggg gcgatgatcg cgctgcagcc catcaccgac 180 gtggcccatc tcgtccatgc cccgctcgcc tgctggggca acggctggga caaccgcggc 240 tcggcctcgt cgggctccga cctctaccgt cgcggcttca ccaccgacct ctccgagctc 300 gacatcgtga tgggccgcgg cgaggccagg ctcttccgtg ccatccgcga agtgatcgcg 360 caggagaacc cggccgcagt cttcgtctat gccacctgcg tgacggcgct catcggcgac 420 gacatcggcg ccgtctgcaa ggccgccgcc gaacggttcg gccgcccggt gatcccgatc 480 aacgtgccgg gctatgtggg ctcgaagaac ctcggcaaca agctgggggt ggacgcgctg 540 gtcgaacatg tcgtggggac gatggagccc gcgacggcga ccgattgcga catcaacatc 600 ctcggcgact tcaacctgtc gggcgaactc tggcaggtga agccgctgct cgaccgcctc 660 ggcatccgca tcctcggctc ggtctcgggg gatgcgcgct atgcgcaggt ggccatgatg 720 caccgggcgc gggtgacgat gctcgtctgc tcgcacgcct tcctgggcat cgcccgcaag 780 ctcgaggacc gctacggcat cccgtggttc gagggcagct tctacggcat ctccgacacg 840 tccgacgcgc tgcggaccct gtgccggatg ctggtcgagc gcggcgcgcc cgcggacctc 900 gtgacccgct gcgaggcgct gatcgccgag gaggaggccc gcacctgggc cgcgctggaa 960 ccgctccgcc ccgccgtcgc cggccggcgc gtgctccttt acaccggcgg gcacaagacc 1020 tggtcggtgg tctcggcgct gcaggaactc ggcatggagg tggtcggcac ctcgatgcgc 1080 aaggccacgc ccggcgaccg cgcgcgcgtc accgagatca tgggcaccga ggcccacatg 1140 tacgagaaca tggcgccgaa ggagatgtat cggatgctgc gggacgcgcg ggccgatgtg 1200 cttatgtcgg gggggcggtc gcagttcgtg gcgctgaagg cccgcgtgcc ctggatcgac 1260 gtgaatcagg aaaagcacga gccctacgca ggctacatgg gcatggtcga tctcgtgcgc 1320 gccatcgacc ggtcgatcaa caacccgatg tgggccgagc tgcgcgaccc cgcgccttgg 1380 gacgtgccgg ccgaagaagc cgccgtgacg cccttcagcc tcgcggccgt tcccggctcg 1440 aaagccgatt tcgaggattg ctga 1464 <210> 11 <211> 876 <212> DNA <213> Nitrogenase (molybdenum-iron) reductase and maturation protein NifH <400> 11 atgggaaaac tccggcagat cgctttctac ggcaagggcg ggatcggcaa gtcgacgacc 60 tcgcagaaca ccctcgcggc actggtcgag atgggtcaga agatcctcat cgtcggctgc 120 gatcccaagg ccgactcgac ccgcctgatc ctgaacacca agctgcagga caccgtgctt 180 cacctcgccg ccgaagcggg ctccgtcgag gatctcgaac tcgaggatgt ggtcaagatc 240 ggctacaagg gcatcaaatg caccgaagcc ggcgggccgg agccgggcgt gggctgcgcg 300 ggccgcggcg tcatcaccgc catcaacttc ctggaagaga acggcgccta tgacgacgtc 360 gactacgtct cctacgacgt gctgggcgac gtggtctgcg gcggcttcgc catgccgatc 420 cgcgagaaca aggcgcagga aatctacatc gtcatgtcgg gcgagatgat ggcgctctat 480 gcggccaaca acatcgccaa gggcatcctg aaatacgcga actcgggcgg cgtgcgcctc 540 ggcggcctga tctgcaacga gcgcaagacc gaccgcgagc tggaactggc cgaggccctc 600 gccgcgcgtc tgggctgcaa gatgatccac ttcgttccgc gcgacaatat cgtgcagcac 660 gccgagctcc gccgcgagac ggtcatccag tatgcgcccg agagcaagca ggcgcaggaa 720 tatcgcgaac tggcccgcaa gatccacgag aactcgggca agggcgtgat cccgaccccg 780 atcaccatgg aagagctgga agagatgctg atggatttcg gcatcatgca gtccgaggaa 840 gaccggctcg ccgccatcgc cgccgccgag gcctga 876 <210> 12 <211> 1368 <212> DNA <213> Nitrogenase FeMo-cofactor scaffold and assembly protein NifN <400> 12 atggcccgcc tcatccaccc cgaccgcgcg ctctcgacca atccgctgaa ggtctcggcc 60 ccgctcggcg ccgccatggc ctatctcggc atcgagggcg cgatcccgct ctttcacggc 120 gcgcagggct gcaccgcctt cgcgatggtc catatggtgc gccacttcaa ggaggcgatc 180 ccgcttcaga ccacggcgat gaacgaggtc tcggcgatcc tcggcggcgg cgaacagatc 240 gaagaggcga tcgagaatct gcggaagcgt gcctccccga agttcatcgg catcgcctcg 300 accgcgctcg tcgagacgcg cggcgaggat atcgcaggcg aactgcgcga gatgctggcc 360 cggcgccgcg actttgcgga tacggcggtg gtctatgcgg ccacaccgga tttcgcgggc 420 gggctcgaag agggctgggc ccgcgcggtc gaagccatca tcgaggcgct ggtgaccgag 480 gggccgcggc ggctccggca ggtgaacctc ctgcccggcg ccaacatgac cgccgccgac 540 atcgaggaga tcgcgggcct gatccgtgcc ttcggcctcc atccgctgat cctgcccgat 600 ctctcgctct cgctcgacgg ccatctggcc gaggactggc gcggccattc gctgggcggc 660 acgcggctgg ccgacattgc gacgatgggc ggctccatcg cgacgctggc gctgggcgag 720 gcgatgcgcc cggcggccga gaagctggcc gctctgggcg tgcccgcgca tgtctttccc 780 tcggtgacgg ggctcaaggc ggtggatgcc ttcgttgcga ccctcatgcg gctttctggg 840 gcggaggtgc ccgcaggcgt ccggcgcgac cgggcgcggc tggcggacgc gatgctcgat 900 gcgcatttcc acatcggcgg cctgaaggtt gccatgggtc tcgacccgga cctcggcctc 960 gcgctcggga gcacgctggc cgccatgggc gccgagctga cggtcgtcgc cagcacggcg 1020 agccccgccg tggaacgcct gccggtcgag gaggtgctga tcggcgatct cggcgatctc 1080 gagcggctgg ccgaagcctc cggcgcgcag cttctgctga cccacgccca cggccggatg 1140 atggccgagc ggctgcatct gccccatgtc cgggcgggtt ttccgatctt cgaccggctg 1200 ggcacgatgg atgcgtgccg caccggatac cgcggcacgc gcgccttcct tttcgagatc 1260 gccaatgccg tgcttgcgca cccgcaccgg ccgcgtccgg aggatttcgg cgccgcccgt 1320 ctctccccgg agttcgacca tgcccccccg ccgcctcaga ctcattga 1368 <210> 13 <211> 540 <212> DNA <213> Nitrogenase FeMo-cofactor synthesis molybdenum delivery protein NifQ <400> 13 atgaaccagt ggagcgctga cctcttcacc ggtgcggcgg ccgaccgggc cgacatggcg 60 gcgatcctcg cccatgcgct gcgcgagcag gcggcggggc ggggggagct gtgcgacctt 120 ctggggctcg agcccgcggc gctcgaggcg ctggtcgcgc gctggtgccc gggcctcacg 180 cttcccgcct tggccccgcc cgggccgctg cccatcgaac agtccgatct ggcgacgctt 240 ctcctctggc ggggcgggcg gaccagcgac gaggcgcgct ggcttgccgc catcctcgcc 300 cgccgcgcgc tcgaggccag ccatctgtgg gaggatctgg gcctgccctc gcgcgcccat 360 ctgggccggt taatcgagcg ccatttcccc cgcctccatg ccgccaatac ccgaaacatg 420 cgctggaagc gcttcttcta ccgccagatc tgcaccgacc atggcggcac gatgtgcctt 480 gcgcccaact gcgaggcctg cgccgagcag ccgctctgct tcggacccga cgccgactga 540 540 <210> 14 <211> 327 <212> DNA <213> Nitrogenase stabilizing/protective protein NifW <400> 14 atgaccccgg gaaccgccgt gctcgaggag ctgaagcgac tgtcctctgc cgaggagatc 60 ttcgacgccc tcgaccatcc ctaccggccg gaggtggtgc aggtcgcgcg cctccatatc 120 atgaagcggc tgggccagta tctcgccgcc gtcgatttcg cgacgctgga tccggccgac 180 gcccgcgccg ccgcgcgcga cgcgctctcg cgggcctata ccgacttcgt ggacagctcg 240 cccctcgagc agaaggtgtt caaggtcttc gccaagccct cgcgcgcctt cgtgccgctc 300 tcgggcctgt cggtggtcga ggactag 327 <210> 15 <211> 435 <212> DNA <213> Nitrogenase FeMo-cofactor carrier protein NifX <400> 15 atgaccgacc cggccgacgt gcccctcagg atcgccatcg cgaccaacga cctcgaaacc 60 ctcaacgccc atttcggctc ctgccggagt ttcgcgctct gggacgtctc ggcgaaatcc 120 gcgcgcttcg tggaggccgt aggcttcgac gagaccaccg atcagggcgg caagcacgac 180 gacagcgccg accgcatcac ccccaaggtc gaggcgctga cgggctgcgc gctcctgttc 240 gtgctggcca tcggcggtcc cgccgcggcc cgcgtggtgc gcgcgggcgt ccatccgatc 300 aagcgcaagg agcccgagcc gatctcggcc atcgtcgctc aggtgcagga catgctgaac 360 ggcaacgtgc cgcccttcct gcgcaaggtg ctcggccgtc ctgcccccag ctttctcgag 420 gaggagacgc gatga 435

Claims (7)

Rhodobacter sphaeroides strain KNU-04 (Accession No. KCTC13065BP) with plant growth promoting effect.
The method according to claim 1,
Characterized in that the strain comprises the 16S rRNA base sequence of SEQ ID NO: 1, the Rhodobacter sphaeroides strain KNU-04 (Accession No. KCTC 13065BP).
The method according to claim 1,
The strain is Rhodobacter sphaeroides strain KNU-04 (Accession No. KCTC 13065BP), which comprises a plant growth hormone function gene having the plant growth promoting ability of SEQ ID NOS: 2 to 5.
The method according to claim 1,
The strain is Rhodobacter sphaeroides strain KNU-04 (Accession No. KCTC 13065BP), which comprises the nitrogen fixative gene having the nitrogen fixation ability of SEQ ID NOS: 6 to 15.
The Rhodobacter spores of claim 1, sphaeroides KNU-04 strain, a culture of the strain, a concentrate of the culture, a dried product of the culture, and a combination thereof.
A microorganism fertilizer for promoting plant growth, comprising the microorganism preparation of claim 5.
A method for promoting plant growth, comprising treating the microbial agent of claim 5 to a soil, plant, or plant seed.

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