KR20180056611A - Premixed of Oat with high beta-glucan for fried dish and Manufacturing Method Thereof - Google Patents
Premixed of Oat with high beta-glucan for fried dish and Manufacturing Method Thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/157—Farinaceous granules for dressing meat, fish or the like
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/50—Polysaccharides, gums
- A23V2250/502—Gums
- A23V2250/5034—Beta-Glucan
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Zoology (AREA)
- Cereal-Derived Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
본 발명은 베타글루칸 함량이 높은 귀리 부침믹스 및 이의 제조방법에 관한 것이다.The present invention relates to an oatmeal mix having a high beta-glucan content and a process for producing the same.
의학기술의 발달로 인한 평균 수명 증가로 최근 삶의 질 향상에 대한 관심이 높아지고 있으며, 이러한 현상은 식품, 건강식품 등의 분야에서도 두드러지게 나타나고 있다. With the increase in life expectancy due to the development of medical technology, there has been a growing interest in improving the quality of life in recent years, and this phenomenon is becoming more prominent in the fields of foods and health foods.
귀리(Avena sativa L.)는 세계 10대 슈퍼푸드 중 하나로 소비자 관심이 높은 작물 중 하나이다. 최근, 귀리에 관한 관심이 높이지면서, 건강한 귀리를 단순하게 혼반하는 수준이 아닌 다양하게 소비할 수 있는 가공소재 개발이 시급한 상황이다. Oats (Avena sativa L.) is one of the top 10 super foods in the world and one of the most popular crops for consumers. In recent years, as interest in oats has increased, it is urgent to develop processing materials that can be consumed variously instead of simply mixing healthy oats.
귀리의 원산지는 중앙 아시아 지역이며, 녹말을 포함한 식용 식물로서 껍질을 벗기고 납작하게 눌러 오트밀이나, 쿠키 등으로 만들어 먹는다. 탈곡과정에서 껍질이 벗겨지면 쌀귀리, 벗겨지지 않으면 겉귀리로 나뉘며, 쌀귀리는 껍질을 벗겨내는 작업을 생략할 수 있어 주로 식용으로 이용되고 있고, 겉귀리는 주로 조사료 생산용으로 이용되고 있다. 내한성과 분얼력(tillering capacity)은 겉귀리가 강하고, 초장은 쌀귀리가 크며, 겉귀리가 재배적 안정성이 높다.Origin of oats is the Central Asian region, which is an edible plant containing starch, peeled, flattened and made with oatmeal, cookies, and the like. When the husks are peeled off during the threshing process, rice oats are separated into oats if they are not peeled off. Rice oats are used mainly for edible purposes because they can be omitted from peeling, and oats are mainly used for the production of forage. Cold tolerance and tillering capacity are strong in the outermost layer, and the plant height is high in rice oats, and the outermost layer is highly cultivated.
식품 의약품 안전처에 따르면, 귀리 식이섬유를 하루 0.8g 이상 섭취 시 식후 혈당상승 억제에 도움을 줄 수 있고, 3g 이상 섭취 시 혈중 콜레스테롤 개선에 도움을 줄 수 있는 등 그 기능성이 인정되고 있다. According to the Food and Drug Administration, oat fiber intake of 0.8g or more a day can help to prevent postprandial hyperglycemia, and it can be helpful to improve blood cholesterol when ingested more than 3g.
또한, 귀리는 면역증강작용을 갖는 베타글루칸((1-3),(1-4)-β-D-glucan)을 함유하고 있다고 알려져 있다. 베타글루칸은 혈중 콜레스테롤 및 LDL-콜레스테롤 농도를 낯추는 동시에 HDL-콜레스테롤 농도를 높이고, 콜레스테롤의 간 축적을 억제하며 지방질의 소화기능을 저하시키는 유용한 생리활성물질이다. 베타글루칸은 체내에서 소화, 흡수되지 않는 식이섬유의 일종으로 효모, 버섯, 곡류 등으로부터 다양한 형태로 생산되고 있다. 일반적으로 베타글루칸은 원료에 따라 구조와 특성이 차이가 있는데, 곡류의 베타글루칸은 주로 세포벽에 존재하며, 그 구조는 β-1,3 및 β-1,4 결합이 다양한 비율로 이루어진 직쇄상의 다당류이다. 이러한 베타글루칸은 귀리나 보리에 다량 함유되어 있으며, 특히 귀리가 높은 베타글루칸을 함유하고 있다. It is also known that oats contain beta-glucans ((1-3), (1-4) -? - D-glucan) which have immunomodulating action. Beta Glucan is a useful physiologically active substance that lowers blood cholesterol and LDL-cholesterol concentration, increases HDL-cholesterol concentration, inhibits cholesterol liver accumulation and lowers lipid digestion function. Beta Glucan is a dietary fiber that is not digested or absorbed in the body and is produced in various forms from yeast, mushroom, and grains. Generally, beta-glucan differs in structure and characteristics depending on the raw material. The beta-glucan of cereal is mainly present in the cell wall, and its structure is a straight-chain type in which β-1,3 and β- It is a polysaccharide. Such beta-glucan is contained in a large amount in the ear or barley, and particularly contains high-beta-glucan.
귀리를 활용한 가공식품으로는 대한민국 공개특허로 분말 귀리를 물에 용해하여 밥을 제조하는 방법(한국공개특허 10-2003-0070513), 귀리 등 혼합 곡물 플레이크를 제조하는 방법(한국공개특허 10-2011-0046896) 및 귀리 즉석밥의 제조(한국공개특허 10-2013-0087863)등이 있으나, 다양한 가공식품으로 제조되는 데에는 아직 한계가 있으며, 귀리 고유의 맛과 향을 살리며 영양학적인 측면까지 고려한 가공식품의 개발을 아직 미미한 실정이다.Processed foods using oats are disclosed in Korean Patent Laid-Open Publication No. 10-2003-0070513, in which powdered oats are dissolved in water to produce rice flour (Korean Patent Laid- (Korean Patent Laid-Open No. 10-2013-0087863). However, there is still a limit to the production of various processed foods, and there is a problem in that the nutritive aspects of oat The development of food is still very limited.
이에, 본 발명자들은 건강성 및 편의성이 향상된 식품 소재를 개발하던 중, 높은 베타글루칸 함량을 갖는 귀리 입도 크기를 확인하고, 이를 이용하여 기능성 및 기호도가 증진된 귀리 부침믹스를 개발함으로써, 상기 귀리 부침믹스가 귀리 소비 증진 및 귀리 가공 식재료로 유용하게 이용될 수 있음을 밝힘으로써 본 발명을 완성하였다.Accordingly, the inventors of the present invention have developed an oat drop mix having improved oat particle size with high beta-glucan content and improved functionality and preference while developing a food material with improved health and convenience, The present invention has been completed by showing that the mix can be usefully used as oat-consuming and oat processing food ingredients.
본 발명의 목적은 혈당지수가 낮으면서 베타글루칸 함량이 높은 귀리 부침믹스 및 이의 제조방법을 제공하는 것이다.It is an object of the present invention to provide an oatmeal mix having a low blood glucose level and a high beta-glucan content and a method for producing the same.
상기 목적을 해결하기 위해, 본 발명은 40 내지 80 mesh의 겉귀리 또는 80 내지 120 mesh 쌀귀리에 쌀가루 또는 밀가루를 혼합하여 제조한 부침믹스 제조방법을 제공한다.In order to solve the above-mentioned problems, the present invention provides a method for manufacturing an infusion mix, which is prepared by mixing rice flour or wheat flour with 40 to 80 mesh oat or 80 to 120 mesh rice oats.
또한, 본 발명은 본 발명의 제조방법으로 제조된 귀리 부침믹스를 제공한다.The present invention also provides an oatmeal mix produced by the method of the present invention.
본 발명은 베타글루칸 함량이 높은 귀리 부침믹스 및 이의 제조방법에 관한 것으로, 구체적으로 귀리 부침믹스 개발을 위해, 베타글루칸 함량이 높은 귀리의 품종별 입도 크기를 조사한 결과, 쌀귀리 100 mesh 및 겉귀리 60 mesh에서 가장 높은 베타글루칸 함량을 나타내었고, 쌀귀리에는 밀가루를, 겉귀리에는 쌀가루를 혼합하는 것이 혈당지수(GI)가 낮은 것으로 나타내었다. 상기 베타글루칸 함량이 높은 귀리와 곡물가루를 다양한 배합비로 혼합한 부침믹스를 이용하여 부침개를 제조하고, 이의 성분, 물성 등을 조사하고, 베타글루칸 함량, 호화특성, 혈당지수 및 기호도 등을 조사한 결과, 베타글루칸 함량이 높은 귀리를 40%로 배합하여 제조된 귀리 부침믹스가 기호도 및 기능성이 가장 우수함을 확인함으로써, 본 발명의 귀리 부침믹스 및 이의 제조방법은 건강식품 소재로 유용하게 이용될 수 있습니다.The present invention relates to an oat drop mix having a high beta-glucan content and a method for producing the oat drop mix, and more particularly, to the development of a oat drop mix, 60 mesh showed the highest content of beta - glucan, while the addition of wheat flour to rice oats and rice flour to the outer rim showed lower glucose index (GI). The content of the beta-glucan, the degree of gelatinization, the blood glucose level and the degree of preference were investigated in the broth using an impregnation mix prepared by mixing the oat and grain powder having a high content of beta-glucan in various mixing ratios, , And the oat drop mix prepared by blending 40% oat with high beta-glucan content has the highest preference and functionality, the oat drop mix of the present invention and its manufacturing method can be used as a health food material .
도 1은 귀리 품종별 분쇄크기에 따른 외관을 나타낸 도이다.
도 2는 베타글루칸 함유량이 높은 귀리 입도 크기 분포를 나타낸 도이며;
도 2a는 겉귀리 베타글루칸 함유량이 높은 입도 크기 분포이고,
도 2b는 쌀귀리 베타글루칸 함유량이 높은 입도 크기 분포이다.
도 3은 귀리 품종별 분쇄크기에 따른 전분 소화율을 나타낸 도이며;
도 3a는 겉귀리의 분쇄크기에 따른 전분 소화율을 나타낸 도이고,
도 3b는 쌀귀리의 분쇄크기에 따른 전분 소화율을 나타낸 도이다.
도 4는 귀리와 곡물 가루(쌀가루 또는 밀가루)의 혼합을 쌀가루 100%, 밀가루:귀리(50:50), 쌀가루:귀리(50:50) 및 생귀리 100%로 혼합하여 반죽을 만들어 부침개(전)를 제조하였을 때 부침개 형태를 확인한 도이다.
도 5는 곡물 가루 대체 귀리가루 혼합비율별 부침개의 외관을 나타낸 도이다.Fig. 1 is a view showing the appearance according to the size of crushing by oat varieties.
2 is a diagram showing an oat grain size distribution with a high beta-glucan content;
2a shows a particle size distribution with a high content of oat beta-glucan,
Figure 2b is a particle size distribution with a high content of rice oat beta-glucan.
Fig. 3 is a graph showing the starch digestibility according to the crushing size of oat varieties; Fig.
FIG. 3A is a graph showing the starch digestibility according to the crushing size of crustacea,
FIG. 3B is a graph showing the starch digestibility according to the crushing size of rice oats.
FIG. 4 shows that a mixture of oats and grain flour (rice flour or wheat flour) is mixed with
Fig. 5 is a view showing the appearance of a chopper according to a mixing ratio of grain powder replacement oat flour.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명은 40 내지 80 mesh의 겉귀리 또는 80 내지 120 mesh 쌀귀리에 쌀가루 또는 밀가루를 혼합하여 제조한 부침믹스 제조방법을 제공한다.The present invention provides a method for manufacturing an infusion mix, which is prepared by mixing rice flour or wheat flour in 40 to 80 mesh oat or 80 to 120 mesh rice oats.
또한, 본 발명은 본 발명의 제조방법으로 제조된 귀리 부침믹스를 제공한다.The present invention also provides an oatmeal mix produced by the method of the present invention.
상기 귀리는 재배한 것 또는 시판되는 것 등 제한 없이 사용할 수 있으며, 동속근연계 식물 어느 것도 이용가능하며 이에 한정하지 않는다.The oats may be cultivated or marketed without limitation, and any plant of the same genus may be used, but the present invention is not limited thereto.
상기 겉귀리는 20 내지 100 mesh로 분쇄하는 것이 바람직하고, 40 내지 80 mesh로 분쇄하는 것이 더욱 바람직하며, 50 내지 70 mesh로 분쇄하는 것이 가장 바람직하며, 본 발명의 바람직한 실시예에 의하면, 베타글루칸 함량이 높은 겉귀리 입도 크기는 60 mesh이다.The crust is preferably pulverized to 20 to 100 mesh, more preferably pulverized to 40 to 80 mesh, most preferably pulverized to 50 to 70 mesh. According to a preferred embodiment of the present invention, The size of the crusted oat grain is 60 mesh.
상기 쌀귀리는 60 내지 140 mesh로 분쇄하는 것이 바람직하고, 80 내지 120 mesh로 분쇄하는 것이 더욱 바람직하며, 90 내지 110 mesh로 분쇄하는 것이 가장 바람직하다. 본 발명의 바람직한 실시예에 의하면, 베타글루칸 함량이 높은 쌀귀리 입도 크기는 100 mesh이다.The rice oats are preferably pulverized to 60 to 140 mesh, more preferably to 80 to 120 mesh, and most preferably to 90 to 110 mesh. According to a preferred embodiment of the present invention, the grain size of rice oats having a high beta-glucan content is 100 mesh.
상기 혼합은 귀리가루가 10 내지 40 중량%인 비율로 혼합하는 것이 가장 바람직하다. 본 발명의 바람직한 실시예에 의하면, 40 중량% 초과의 귀리가루가 혼합되는 경우 부침개로서 형태 유지가 어렵다는 단점이 있으며, 40 중량%로 혼합하는 것이 가장 베타글루칸 함량이 높으며, 기호도가 우수하였다.It is most preferable that the mixing is carried out at a ratio of 10 to 40% by weight of the oat flour. According to a preferred embodiment of the present invention, when the oat flour of more than 40% by weight is mixed, there is a disadvantage that it is difficult to maintain shape as a flap, and when mixed at 40% by weight, the content of beta-glucan is the highest and the preference is excellent.
상기 부침믹스는 혈당지수(GI) 또는 전분소화율이 낮은 것을 특징으로 하며, 본 발명의 바람직한 실시예에 의하면, 밀가루에는 쌀귀리를, 쌀가루에는 겉귀리를 혼합하는 것이 혈당지수(GI)가 낮은 기능성 부침믹스 개발에 효과적이다.According to a preferred embodiment of the present invention, mixing the rice oats with wheat flour and oat flour with rice flour is effective in lowering the glucose index (GI) It is effective in development of infusion mix.
본 발명에 따른 부침믹스는 필요에 따라 조제 전분을 추가로 포함할 수 있으며, 타피오카 조제전분, 글리신, 덱스트린 등이 포함된다. 또한, 부침믹스는 적정량의 옥수수가루, 지미강화베이스, 베이킹파우더, 설탕, 소금, 양파분말, 마늘분말, 간장분말, 후추분말, 비타민 B2와 같은 통상적인 재료 5 내지 10중량%를 추가로 포함할 수 있다.The infusion mix according to the present invention may further comprise prepared starch, if necessary, and includes tapioca starch, glycine, dextrin and the like. In addition, the infusion mix further comprises 5 to 10% by weight of conventional ingredients such as an appropriate amount of corn flour, Jimmy fortified base, baking powder, sugar, salt, onion powder, garlic powder, soy flour, pepper powder, .
또한, 본 발명에 따른 부침믹스 조성물은 임의로 파, 양파, 마늘, 애호박, 감자, 부추, 실파, 당근, 고추로부터 선택되는 건조된 채소를 추가로 포함할 수 있으며, 부침의 맛을 좋게 하기 위하여 소금, 간장, 후추, 고춧가루, 생강, 양파, 마늘로부터 선택되는 향신 분말을 추가로 포함할 수 있다. 상기 건조 채소는 용도에 따라 임의의 배합비로 부침믹스에 포함되거나, 또는 따로 포장되어 제공될 수 있다.In addition, the infusion mix composition according to the present invention may further optionally contain a dried vegetable selected from par, onion, garlic, zucchini, potato, leek, green onion, carrot and pepper, , Soy sauce, pepper, red pepper powder, ginger, onion, and garlic. The dry vegetable may be included in the infusion mix at an arbitrary ratio depending on the use, or may be provided separately packaged.
본 발명의 구체적인 실시예에서, 본 발명자들은 베타글루칸 함량이 높은 귀리 부침믹스를 만들기 위해 귀리 품종별 일반 성분을 조사하였으며, 입도 크기에 따른, 색도 및 베타글루칸 함량을 조사하고, 품종별 분쇄크기에 따른 입도, 조지방 함량, 총전분, 아밀로오스 함량, 호화특성, 전분 소화율 및 eGI를 조사하였다. 그 결과, 귀리 부침믹스 제조를 위해 베타글루칸 함량이 높은 입도(mesh 별)를 겉귀리 60 mesh, 쌀귀리 100 mesh로 선정하였다(표 1 내지 표 10 참조).In a specific embodiment of the present invention, the present inventors investigated the general components of the oat varieties to make an oatmeal mix having a high content of beta-glucan, and examined the chromaticity and beta-glucan content according to the particle size, Crude fat content, total starch, amylose content, gelatinization characteristics, starch digestibility and eGI were investigated. As a result, grain size (mesh star) having a high content of beta-glucan was selected as 60 mesh of outer oats and 100 mesh of rice oats for the preparation of oat drop mix (see Tables 1 to 10).
또한, 상기 베타글루칸 함량이 높은 입도크기를 갖는 겉귀리(60 mesh) 및 쌀귀리(100 mesh)를 이용하여, 다양한 배합비로 혼합하여 부침개로 제조하였다. 이때, 곡물가루로 쌀가루 또는 밀가루를 혼합하였으며, 귀리가루의 혼합이 50% 이상인 경우 부침개로서의 형태 유지가 어려워, 귀리 부침개 제조 및 품질 검사에서는 귀리 함유량을 40% 이내로 배합하여 귀리를 제조하였다(도 4 참조).In addition, 60 mesh and oat (100 mesh) having a particle size of a high content of beta-glucan were mixed at various mixing ratios and made into a blender. In this case, rice flour or wheat flour was mixed with the grain flour. When the oat flour mixture was more than 50%, it was difficult to maintain its shape as a clogger. In oat flour production and quality inspection, oats were produced by mixing the oat flour content within 40% Reference).
상기와 같이 다양한 배합비로 제조된 부침개(전)의 외관, 색도, 흡유율, 조지방, 수분함량, 물성을 조사하고(표 11 내지 표 13 참조), 귀리 혼합비율별 부침개의 베타글루칸 함량 및 총전분 함량을 분석한 결과, 귀리의 혼합비율이 증가함에 따라 베타글루칸 함량이 증가함을 알 수 있었으며, 품종에 따른 함량 차이는 나타나지 않았다(표 14 참조). 따라서 부침믹스의 선정 기준인 베타클루칸 함량으로 볼 때 곡물가루와 귀리가루의 적정혼합비는 40%가 적정한 것으로 판단되었다.The appearance, color, absorbency, crude fat, moisture content and physical properties of the broth prepared in various mixing ratios were examined (see Tables 11 to 13), and the content of betaglucan and total starch As a result of analysis of content, it was found that the content of betaglucan was increased by increasing the mixing ratio of oats, and there was no difference in content according to the kinds of cultivars (see Table 14). Therefore, it was concluded that 40% of the optimum mixing ratio of cereal flour and oat flour is appropriate in terms of the content of betaclucan, which is the selection criterion of the infusion mix.
또한, 귀리 혼합비율별 부침개의 호화특성, 전분 소화율, eGI 및 기호도를 검사하였으며, 곡물첨가 귀리부침가루 믹스의 적정 곡물 선정을 위해 eGI 값을 살펴 본 결과, 밀가루 베이스일 때는 쌀귀리 부침믹스가, 쌀가루 베이스일 때는 겉귀리 부침믹스에서 eGI값이 낮게 나타남을 확인하여, 밀가루에는 쌀귀리를, 쌀가루에는 겉귀리를 혼합하는 것이 혈당지수(GI)가 낮은 기능성 부침믹스 개발에 효과적임을 확인하였다(표 18 참조).The eGI value for the selection of the proper grains of the cereal added oat flour mixture was investigated. As a result, the rice oat flour mix at the flour base, It was confirmed that the eGI value was lower in the oat flour mix for the rice flour base, and it was confirmed that mixing the oat flour with rice oat and the rice flour with the oat flour were effective in developing functional immersion mix with low glucose index (GI) 18).
또한, 기호도 검사 결과, 곡물가루 대비 귀리첨가에 따라 전반적으로 기호도가 좋아지는 처리구는 쌀가루 첨가대비 겉귀리 40%가 가장 좋았고, 쌀귀리 10 내지 40%간에는 유의적인 차이가 없었다. 이에, 귀리첨가 믹스의 적정 배합비는 함량이 높은 40%가 적정할 것으로 판단하였다(표 19 참조). 따라서, 본 발명의 혈당지수가 낮으면서 베타글루칸 함량이 높은 귀리 부침믹스 및 이의 제조방법은 귀리 원료를 이용한 우리 고유 식품의 활용 범위를 확대시키며, 건강식품 소재로 유용하게 이용될 수 있다.As a result of the preference test, 40% of the oat flour was the best in the treatments which showed better overall preference according to the addition of oats compared to the grain flour, but there was no significant difference between 10 and 40% of the rice oat. Therefore, it was judged that the optimum mixing ratio of the oat-added mix was proper at 40% (see Table 19). Accordingly, the oat drop mix having a low blood sugar level and a high beta-glucan content and a manufacturing method thereof according to the present invention broaden the application range of the native food using the oat ingredient and can be usefully used as a health food material.
이하, 본 발명을 실시예 및 실험예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.
단, 하기 실시예 및 실험예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예 및 실험예에 의하여 한정되는 것은 아니다.However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following Examples and Experimental Examples.
<실시예 1> 귀리 품종별 귀리의 일반성분 조사<Example 1> Examination of general composition of oats according to oat varieties
본 발명에서 2014년도 전북 정읍 귀리명품화사업단에서 생산한 쌀귀리(조양)와 겉귀리(조한) 품종을 사용하였으며, 대조구로는 수입산 귀리(바른약초, 캐나다산)를 시중에서 구입하여 사용하였다.In the present invention, rice oats (Chaiyang) and oat (Chojang) cultivars produced by the Jeonbuk Jeongeup Oat Rice Project in Jeonbuk Province in 2014 were used, and imported oats (barley herb, Canadian) were purchased from the market.
베타글루칸 함량이 높은 귀리 부침믹스를 만들기 위해 귀리 품종별 수분함량, 회분, 조지방, 조단백 및 식이섬유를 측정하였다. 구체적으로, 수분함량, 회분, 조지방, 조단백 등의 일반성분은 AOAC의 표준분석법에 준하여 수분은 105℃ 상압 가열 건조법으로 측정하였고, 조단백질은 Kjeldahl법, 조섬유는 건식회화법, 조지방은 Soxhlet추출법으로 분석하였으며 회분은 600℃ 회화로에서 직접 회화시켜 중량법으로 정량하였다. 탄수화물은 수분, 조단백질, 조지방, 회분의 함량(%)의 합을 100%로부터 뺀 값으로 구하였다. 식이섬유는 조섬유 추출기(Fibertek 1023 system, Foss SWE)를 사용하여 추출하였다. 분말 1.0±0.005 g에 MES/TRIS 용액 40mL를 가한 후 충분히 분산시켜 α-amylase 50㎕을 가하여 혼합시킨 후 95℃의 수욕(물중탕)에서 교반하여 40분간 유지하고 60℃로 냉각한 후, protease 100 ㎕를 가해 60℃에서 교반하면서 30분간 수욕에서 항온하였다. 그런 다음, 0.561N HCl 5 mL를 가하여 혼합한 후, 60℃에서 pH 4.0~4.7로 조정하였다. 그 후, amyloglucosidase 200 ㎕를 가한 후, 60℃에서 30분간 교반한 뒤 항온시켜 시험 용액으로 사용하였다. 시험용액에 60℃의 95% 에탄올 200 mL를 가하고 실온에서 1시간 방치하여 침전시켰다. 항량(constant weight)시킨 유리여과기에 셀라이트 0.5 g을 넣어 78% 에탄올 15 mL를 가하여 분산시킨 후, 시험용액을 넣어 여과하고, 용기의 잔류물은 78% 에탄올로 씻어주었다. 잔사는 78% 에탄올, 95% 에탄올, 아세톤 순으로 각각 2회 세척하고 105℃ 건조기에서 하루 동안 건조시켰다. 방냉 후, 무게를 측정하여 하나의 여과기 잔사는 조단백을, 다른 하나의 여과기 잔사는 조회분을 실시하여 식이섬유 함량을 하기의 식을 이용하여 계산하였다.Moisture content, ash, crude fat, crude protein and dietary fiber of oat varieties were measured to produce an oatmeal mix with high beta - glucan content. Specifically, the moisture content, ash, crude fat, crude protein and other general components were measured by the 105 ° C atmospheric pressure heating method according to AOAC standard method. The crude protein was analyzed by Kjeldahl method, the crude fiber was analyzed by dry method and the crude fat was analyzed by Soxhlet extraction method And the ash was quantitated by weight method directly in a painting furnace at 600 ℃. Carbohydrates were obtained by subtracting the sum of the contents (%) of moisture, crude protein, crude fat and ash from 100%. Dietary fiber was extracted using a fiber-optic extractor (Fibertek 1023 system, Foss SWE). After adding 40 μl of MES / TRIS solution to 1.0 ± 0.005 g of powder, 50 μl of α-amylase was mixed and dispersed. The mixture was stirred in a water bath (95 ° C) for 40 minutes, cooled to 60 ° C, And the mixture was incubated in a water bath for 30 minutes with stirring at 60 ° C. Then, 5 mL of 0.561N HCl was added and mixed, and the pH was adjusted to 4.0 to 4.7 at 60 ° C. After that, 200 μl of amyloglucosidase was added, and the mixture was stirred at 60 ° C for 30 minutes, and then the mixture was incubated and used as a test solution. To the test solution was added 200 mL of 95% ethanol at 60 DEG C and allowed to stand at room temperature for 1 hour to precipitate. 0.5 g of celite was added to a constant weight glass filter, and 15 mL of 78% ethanol was added to disperse it. Then, the test solution was added to the filtrate, and the residue of the container was washed with 78% ethanol. The residue was washed twice with 78% ethanol, 95% ethanol and acetone, respectively, and dried in a 105 ° C dryer for one day. After cooling, weighing was performed, and one filter residue was subjected to crude protein and the other filter residue was subjected to aspiration, and the dietary fiber content was calculated using the following equation.
식이섬유 함량(%) = (검체의 평균잔사 무게(㎎)-P-A-B)/검체의 평균 무게(㎎) *100(%) = (Mean residue weight of specimen (mg) -P-A-B) / average weight of specimen (mg) * 100
P: 단백질량(㎎), A: 회분량(㎎), B: 공시험 값(㎎)P: protein amount (mg), A: ash content (mg), B: blank test value (mg)
그 결과 표 1에 나타난 바와 같이, 곡류의 대표적인 쌀을 대조구로 일반성분을 비교한 결과, 탄수화물은 쌀(91.48%)의 함량이 가장 많았으며 품종별 수입산 귀리(79.89%), 쌀귀리(73.33%), 겉귀리(74.93%) 순으로 나타났다(표 1). 일반성분 중 회분, 조지방, 조단백의 함량은 쌀귀리, 겉귀리, 수입산 귀리 순으로 함량이 높게 나타났으며 이는 쌀보다 모두 높은 수치였다.As a result, as shown in Table 1, the content of rice (91.48%) was the highest in the carbohydrate, the imported oats (79.89%), the rice oats (73.33% ) And oat (74.93%), respectively (Table 1). The contents of ash, crude fat and crude protein were higher in rice than those of rice, oat, oat, and imported oat.
1)같은 행에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets in the same row mean a significant difference from each other at Duncan's multiple range test at p <0.05.
또한, 표 2에 나타난 바와 같이, 불용성 및 수용성 식이섬유의 함량은 귀리 품종별 유의적인 차이는 나타나지 않았지만, 쌀에 비해 약 10배 정도 높은 수치를 나타냄을 확인하였다(표 2).In addition, as shown in Table 2, insoluble and soluble dietary fiber content was about 10 times higher than that of rice, although there was no significant difference between the oat varieties (Table 2).
1)같은 행에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets in the same row mean a significant difference from each other at Duncan's multiple range test at p <0.05.
<실시예 2> 귀리 품종별 입도 크기에 따른 색도 확인<Example 2> Confirmation of Colors According to Particle Sizes of Oat Varieties
귀리 품종별 입도 크기에 따른 색도를 확인하고자, pin mil을 이용하여 귀리를 갈았고, 배출체의 mesh를 달리하여 각각 40, 60, 80, 100 mesh의 귀리가루를 제조하였다.Oat flour was prepared with 40, 60, 80, and 100 mesh, respectively, by using a pin mill to grind the oats and to differentiate the mesh of the discharge body.
구체적으로. 일정량의 시료를 색차계(Chroma Meter, CR-300, Minolta Co., Tokyo, Japan)를 이용하여 제조사의 지침을 따라 L(lightness), a(redness), b(yellowness) 값을 측정하였다.Specifically. L (lightness), a (redness) and b (yellowness) were measured by using a chromatic meter (CR-300, Minolta Co., Tokyo, Japan) according to the manufacturer's instructions.
그 결과 표 3에 나타난 바와 같이, L값은 겉귀리가 쌀귀리에 비해 밝았으며 mesh가 클수록 밝기가 증가하는 경향을 나타냈으며 그 중 80 mesh로 분쇄한 귀리분말의 밝기가 가장 밝았다(표 3). 또한, a값은 겉귀리가 쌀귀리에 비해 전체적으로 높았으며 mesh가 작을수록 높은 경향을 나타낸 반면, b값은 쌀귀리가 겉귀리에 비해 높게 나타났으며 mesh가 클수록 높은 수치를 나타내었다(표 3).As a result, as shown in Table 3, the L value was higher than that of rice oats, and the brightness increased with increasing mesh size, and the brightness of oat powder crushed by 80 mesh was the brightest (Table 3). The value of a was higher than that of rice oats, while the value of a was higher than that of rice oats, while the value of b was higher than that of rice oats (Table 3).
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
<실시예 3> 귀리 품종별 분쇄크기에 따른 입도 분석≪ Example 3 > Particle size analysis according to crushing size by oat varieties
귀리 품종별 분쇄크기에 따른 입도 크기를 분석하였다.The grain sizes of oat varieties were analyzed according to their size.
구체적으로, 분쇄한 귀리가루의 입도분석은 Particle Size Analyzer(HELOS, Berlin, Germany)를 이용하여 귀리가루 분말 2g을 취하여 분산시킨 후 3회 반복하여 입자의 크기를 측정하였다.Specifically, the particle size of pulverized oat flour was measured by repeating 3 times repeatedly after taking 2 g of oat flour powder using Particle Size Analyzer (HELOS, Berlin, Germany).
그 결과 표 4 및 도 1에 나타난 바와 같이, 쌀귀리가 곁귀리 품종에 비해 분쇄크기에 따른 입자크기가 전반적으로 높게 나타났으며, 분쇄 mesh가 작을수록 입도가 큰 경향을 나타내었으나, 80 mesh와 100 mesh는 유의적인 차이를 나타내지 않았다(도 1 및 표 4).As a result, as shown in Table 4 and FIG. 1, the grain size according to the crushing size was generally higher in the oats of rice oats than in the side oats, and the smaller the crushing mesh, the greater the grain size. 100 mesh showed no significant difference (FIG. 1 and Table 4).
1)같은 행에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets in the same row mean a significant difference from each other at Duncan's multiple range test at p <0.05.
<실시예 4> 귀리 품종별 입도 크기에 따른 베타글루칸 함량 확인Example 4 Confirmation of Beta Glucan Content According to Particle Sizes of Oat Varieties
베타글루칸 함량이 우수한 귀리를 선별하기 위해, 귀리 품종별 입도 크기에 따른 베타글루칸 함량을 확인하였다.In order to select oats with excellent beta - glucan content, beta - glucan content was determined by grain size according to oat varieties.
먼저, 분쇄 크기에 따른 베타글루칸 함량을 확인하고, 귀리 품종 및 분쇄크기에 따른 베타글루칸 함유량이 높은 입도 분포를 확인하였다. First, the content of betaglucan was determined according to the crushing size, and the particle size distribution of betaglucan content was determined according to oat varieties and crushing size.
구체적으로, 베타글루칸 함량은 총 함량, 지용성 및 수용성 함량을 Megazyme kit(K-BGLU)를 이용하여 측정하였다. 시료 500 mg에 50%(v/v) 에탄올 1 mL을 첨가 및 교반한 후 20 mM 인산나트륨 완충용액(sodium phosphate buffer)(pH6.5) 4.0 mL을 가하고 100℃에서 6분간 반응하였다. 추출액에 리체나아제(lichenase)(10 unit) 0.2 ml을 첨가한 후 40℃에서 1시간 효소처리 하였고, 증류수를 이용해 30 mL 정용하여 원심분리(1,000 × g, 10 min)하여 상등액을 취하였다. 상등액 0.1 mL을 각각 3개의 시험관에 담고 하나의 시험관에는 50 mM 아세트산나트륨 완충용액(sodium acetate buffer)(pH 4.0) 0.1 mL, 나머지 두 개의 시험관에는 베타글루코시다아제(β-glucosidase) 0.1 mL을 가한 후 40℃에서 15분 반응하였다. 반응액에 Glucose oxidase/peroxidase 시약 3 mL을 가하고 40℃에서 20분간 반응 후 510 nm에서 흡광도를 측정하여 최종적으로 베타글루칸 함량을 구하였다.Specifically, the content of beta-glucan was determined by using a Megazyme kit (K-BGLU). To 500 mg of the sample, 1 mL of 50% (v / v) ethanol was added and stirred, 4.0 mL of 20 mM sodium phosphate buffer (pH 6.5) was added, and the mixture was reacted at 100 ° C. for 6 minutes. 0.2 ml of lichenase (10 units) was added to the extract, and the mixture was treated at 40 ° C for 1 hour. The supernatant was centrifuged (1,000 × g, 10 min) using 30 ml of distilled water. 0.1 mL of the supernatant is added to each of three test tubes, 0.1 mL of 50 mM sodium acetate buffer (pH 4.0) is added to one test tube, and 0.1 mL of β-glucosidase is added to the remaining two test tubes Followed by reaction at 40 ° C for 15 minutes. 3 mL of glucose oxidase / peroxidase reagent was added to the reaction solution, and the reaction was carried out at 40 ° C for 20 minutes. Then, the absorbance was measured at 510 nm, and the content of beta-glucan was finally determined.
그 결과 표 5에 나타난 바와 같이, 베타글루칸 함량은 겉귀리 3.20~4.23%, 쌀귀리 3.40~4.26%로 나타났으며, 40 mesh를 제외하고 겉귀리는 mesh가 증가할수록 총 베타글루칸 함량이 낮게 나타난 반면, 쌀귀리는 증가하는 경향을 나타내었다(표 5). 지용성 베타글루칸은 mesh가 증가할수록 증가하였으며 수용성 베타글루칸의 비율은 낮아지는 경향을 확인하였다.As a result, as shown in Table 5, the content of beta-glucan was 3.20 ~ 4.23% in the oat, 3.40 ~ 4.26% in the oat of rice, and the total beta-glucan content was lower in the oat except for 40 mesh On the other hand, rice oats showed an increasing tendency (Table 5). It was confirmed that the fat soluble beta - glucan increased with increasing mesh and the water - soluble beta - glucan ratio decreased.
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
또한, 도 2 및 표 6에 나타난 바와 같이, 분쇄크기별 귀리가루의 입도 및 베타글루칸 함량을 분석한 결과, 베타글루칸의 함량이 입자크기에 따라 각각 달랐으며 이를 입도누적곡선분포의 중앙값을 기준으로 5%(45-55%), 10%(40-60%) 분포범위를 설정한 후 베타글루칸 함량이 높은 입도분포 영역을 선정하였다. 즉, 쌀귀리 100 mesh(150 ㎛), 겉귀리 60 mesh(250 ㎛)에서 베타글루칸 함량이 높은 입도분포를 갖는 것을 확인하였다(도 2 및 표 6).As shown in FIG. 2 and Table 6, the content of beta-glucan was different depending on the particle size and the content of beta-glucan was 5 (45-55%) and 10% (40-60%), respectively, and the particle size distribution region with high beta-glucan content was selected. That is, it was confirmed that the content of beta-glucan was high in the
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
이에, 상기 실시예 및 실험예의 결과를 바탕으로 기호성 및 기능성이 향상된 귀리부침가루 개발을 위해 입도별 베타글루칸 함량이 가장 높은 구간으로 겉귀리는 60 mesh, 쌀귀리는 100 mesh를 선정하였다.Based on the results of Examples and Experimental Examples, 60 grams of oat flour and 100 grams of oat rice were selected for the development of oat flour powder having improved palatability and functionality.
<실시예 5> 귀리 품종 및 분쇄크기에 따른 조지방 함량 확인<Example 5> Determination of crude fat content according to oat varieties and pulverization size
분쇄크기에 따른 조지방 함량을 확인하였다.The crude fat content according to the crushed size was confirmed.
구체적으로, 상기 <실시예 1>의 조지방 측정방법과 동일한 방법으로 40, 60, 80 및 100 mesh의 분쇄크기로 분쇄된 쌀귀리 및 겉귀리의 조지방을 측정하였다.Specifically, the crude fat of rice oats and crusted oats crushed at a crushing size of 40, 60, 80, and 100 mesh was measured in the same manner as the crude fat measurement method of Example 1 above.
그 결과 표 7에 나타난 바와 같이, 쌀귀리의 조지방은 8.77~9.54%이며, 겉귀리는 7.60~8.95%로 쌀귀리의 조지방 함량이 전반적으로 높게 나타났으며, 귀리 품종별 분쇄 크기에 따른 조지방 함량에 대하여 경향을 나타나지 않았으나 유의적인 차이를 나타내었다(표 7).As a result, as shown in Table 7, crude fat content of rice oats was 8.77 ~ 9.54% and that of oats was 7.60 ~ 8.95%. Overall, crude fat content of rice oats was high, and crude fat content (Table 7). However, there was no significant difference between the two groups.
1)서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets mean a significant difference from each other at Duncan's multiple range test at p <0.05.
<< 실시예Example 6> 귀리 품종별 분쇄크기에 따른 6> Depending on the crushing size of oat varieties 총전분Total starch , 아밀로오스 함량 및 호화특성 확인, Amylose Content and Characterization
귀리 품종 및 입도크기별 총전분 및 아밀로오스 함량을 확인하였다.Total starch and amylose content were determined by oat varieties and grain sizes.
구체적으로, 총전분 및 아밀로오스 함량은 각각 Megazyme kit(K-TSTA) 및 Megazyme kit(K-AMYL)를 사용하였으며, 간략한 실험 방법은 다음과 같다.Specifically, total starch and amylose content were determined by the Megazyme kit (K-TSTA) and Megazyme kit (K-AMYL), respectively.
귀리의 총전분 분석은 시료 100 mg에 80%(v/v) 에탄올 0.2 mL과 dimethyl sulphoxide 2 mL을 첨가 한 후, thermostable α-amylase 용액을 3 mL 가하고 100℃에서 6분간 반응하였다. 이후, 아밀로글루코시다아제(amyloglucosidase) 0.1 mL을 가하고 50℃ 에서 30분간 반응하였다. 반응이 끝난 시료는 100 mL로 정용하고 원심분리하여 상등액 0.1 mL에 Glucose oxidase/peroxidase 시약 3 mL을 첨가한 후, 50℃에서 20분간 반응하고 510 nm에서 흡광도를 측정하여 전분 함량을 구하였다. 표준물질로는 D-글루코즈(D-glucose)가 사용되었으며, 대조군으로는 옥수수 전분이 사용되었다. Total starch analysis of oats was performed by adding 0.2 mL of 80% (v / v) ethanol and 2 mL of dimethyl sulphoxide to 100 mg of sample, adding 3 mL of thermostable α-amylase solution and reacting at 100 ° C for 6 minutes. Then, 0.1 mL of amyloglucosidase was added and the mixture was reacted at 50 DEG C for 30 minutes. The reacted samples were diluted to 100 mL, centrifuged, and added with 3 mL of the glucose oxidase / peroxidase reagent to 0.1 mL of the supernatant, reacted at 50 ° C for 20 minutes, and the absorbance at 510 nm was measured to determine the starch content. D-glucose was used as a standard substance and corn starch was used as a control group.
귀리의 아밀로오스 함량 분석은 시료 20 mg에 1 mL dimethyl sulphoxide을 첨가한 후 15분간 반응시키고, 95% 에탄올 6 mL을 첨가한 후 100℃에서 15분간 반응시켰다. 이후, 2000×g에서 5분간 원심분리하여 침전물을 25 mL concanavalin A 용매에 녹인 후 0.5 mL concanavalin A 시약으로 1시간 동안 반응시켰다. 반응이 종료된 시료는 14000×g, 10분간 원심분리하여 1 mL의 상등액에 3 mL 100 mM sodium acetate buffer (pH 4.5)및 0.1 mL amyloglucosicase-amylase 용액을 첨가하여 반응시켰다. 이를 다시 2000×g에서 5분 동안 원심분리하여 상등액 1 mL을 취하고 4 mL의 Glucose oxidase/peroxidase 시약과 40℃에서 30분간 반응시킨 후, 510 nm에서 흡광도를 측정하였다.To analyze the content of amylose in oats, 1 mL of dimethyl sulphoxide was added to 20 mg of sample, and the reaction was carried out for 15 minutes. Then, 6 mL of 95% ethanol was added and reacted at 100 ° C. for 15 minutes. After centrifugation at 2000 × g for 5 minutes, the precipitate was dissolved in 25 mL concanavalin A solvent and reacted with 0.5 mL of concanavalin A reagent for 1 hour. After the reaction was completed, the sample was centrifuged at 14,000 × g for 10 minutes, and 3 mL of 100 mM sodium acetate buffer (pH 4.5) and 0.1 mL of amyloglucosicase-amylase solution were added to 1 mL of the supernatant. After centrifugation at 2000 × g for 5 minutes, 1 mL of supernatant was taken and reacted with 4 mL of glucose oxidase / peroxidase reagent at 40 ° C. for 30 minutes, and the absorbance was measured at 510 nm.
또한, 호화특성은 신속점도측정기(RVA, Rapid Visco Analyser-4, Newport Scientific Pty, Ltd., Warriewood NSW, Australia)를 사용하여 측정하였다. 알루미늄 용기에 시료(14% 수분함량 기준) 3 g을 증류수 25 ml에 분산시킨 다음 빠르게 교반시켜 시료액을 제조하였다. 귀리가루의 수분을 각각 측정하여 수분 14% 기준의 메뉴얼에 따라 곡류의 종류 및 수분함량에 따라 증류수양을 달리하여 총수분량을 보정하였다. 호화조건은 초기온도를 50℃에서 1분간 유지한 다음 분당 12℃씩 95℃까지 가열하고, 95℃에서 2분 30초간 유지시킨 다음, 1분당 12℃씩 50℃까지 냉각시켜 2분간 유지시키면서 값을 측정하였다. RVA viscogram으로부터 최고점도(Peak viscosity), 최저점도(trough viscosity), 최종점도(final viscosity), 호화온도(pasting temperature), breakdown (peak-trough), setback(final-trough), 및 호화개시온도(peak time)를 구하여 비교하였으며, 점도단위는 Rapid Viscosity Unit(RVU)로 표시하였다.In addition, the enrichment characteristics were measured using an RVA (Rapid Visco Analyzer-4, Newport Scientific Pty, Ltd., Warriewood NSW, Australia). 3 g of a sample (based on 14% moisture content) was dispersed in 25 ml of distilled water in an aluminum container, followed by rapid stirring to prepare a sample liquid. The moisture content of oat flour was measured. The amount of distilled water was varied according to the type of cereal and water content according to the manual of 14% moisture content, and the total water amount was corrected. The gelatinization condition was maintained at 50 ° C for 1 minute, then heated to 95 ° C at 12 ° C per minute, maintained at 95 ° C for 2 minutes and 30 seconds, cooled to 50 ° C at 12 ° C per minute, Were measured. From the RVA viscogram, peak viscosity, trough viscosity, final viscosity, pasting temperature, breakdown (peak-trough), setback (final-trough) peak time), and the viscosity was expressed as a Rapid Viscosity Unit (RVU).
그 결과, 표 8에 나타난 바와 같이, 총 전분은 전체적으로 mesh가 증가할수록 낮아지는 경향을 나타내었으며, 겉귀리(63.64 ~ 69.82%)가 쌀귀리(52.45 ~ 63.71%) 품종에 비해 총전분 함량이 높게 나타났다(표 8).As a result, as shown in Table 8, total starch showed a tendency to decrease as the mesh was increased, and the total amount of starch (63.64 ~ 69.82%) was higher than that of rice oats (52.45 ~ 63.71% (Table 8).
또한, 아밀로오즈 함량은 겉귀리 품종이 쌀귀리에 비해 전체적으로 높았으며 겉귀리의 분쇄크기별 아밀로오즈는 mesh가 증가할수록 높게 나타난 반면, 쌀귀리의 아밀로오즈 함량은 40 mesh가 가장 낮았으며 60, 80, 100 mesh의 아밀로오즈 함량은 유의적인 차이를 보이지 않았다(표 8).Amylose contents of the oats were higher than those of rice oats. Amylose content of oat crumbs was higher than that of rice oats, while amylose content of rice oats was the lowest , 80 and 100 mesh showed no significant difference (Table 8).
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
또한, 표 9에 나타난 바와 같이, 분쇄크기별 호화특성을 비교한 결과 전반적으로 겉귀리가 쌀귀리에 비해 높게 나타났으며 특히 품종과 관계없이 최고점도, 최소점도, 강하점도는 100 mesh에서 가장 높게 나타났다(표 9). 아울러 호화 개시온도는 40 mesh가 겉귀리는 83.72℃, 쌀귀리가 87.48℃로 가장 높게 나타났으며 60~100 mesh는 유의적 차이를 보이지 않았다(표 9).In addition, as shown in Table 9, compared with the oat of rice, the overall appearance was higher than that of the oat, and the peak, the minimum viscosity, and the falling viscosity were highest at 100 mesh regardless of the varieties Table 9). In addition, there were no significant differences in the initial initiation temperature of 40 mesh, 83.72 ° C of outer oats, and 87.48 ° C of oats of rice and 60 ~ 100 mesh.
(mesh)smash
(mesh)
(Peak viscosity)(RVU)Peak
Peak viscosity (RVU)
(RVU)Breakdown
(RVU)
(Final Viscosity)(RVU)Final viscosity
(Final Viscosity) (RVU)
(RVU)Setback
(RVU)
(min)Peak Time
(min)
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
<실시예 7> 귀리 품종별 분쇄크기에 따른 전분 소화율 및 eGI 확인<Example 7> Determination of starch digestibility and eGI according to crushing size of oat varieties
귀리 품종별 분쇄크기에 따른 전분 소화율 및 eGI를 확인하였다.Starch digestibility and eGI were determined according to crushing size of oat varieties.
구체적으로, 전분 소화율은 Englyst 등의 방법을 수정하여 분석하였으며 0.45 g의 porcine pancreatin(P-7545, Sigma Aldrich, St. Louis, MO, USA)을 증류수 4 mL에 분산시키고 원심분리(2,000 rpm, 10분)한 후, 2.7 mL의 상층액을 분리하였다. 0.32 mL amyloglucosidase(A-7095, Sigma-Aldrich)는 증류수 0.4 mL과 혼합한 후 0.4 mL의 희석된 amyloglucosidase을 취하여, 앞서 제조한 2.7 mL의 porcine pancreatin 상등액을 혼합하여 전분 분해효소를 제조하였다. 전분시료 100 mg에 2 mL의 0.05 M HCl과 0.01 g pepsin, 10개의 유리구슬(4 mm 지름)을 넣고 일정한 속도에서 교반(150 rpm) 한 후 2 mL 0.5 M sodium acetate buffer(pH 5.2)와 미리 제조한 전분 분해효소 1 mL을 넣고 37℃에서 반응시켰다. 각 시간(0-180분)마다 시료(0.1 mL)를 취하여 80% 에탄올용액과 혼합한 후 glucose oxidase and peroxidase assay kits(GAGO-20, Sigma-Aldrich)를 이용하여 glucose 함량으로 측정하였다. 측정된 glucose 양과 total starch 양을 이용하여 20분 내에 분해된 전분함량을 RDS(rapidly digestible starch), 20분과 120분 사이에 분해된 전분함량을 SDS(slowly digestible starch), 120분 동안 분해되지 않는 전분함량을 RS(resistant starch)로 분석하였다. The starch digestibility was determined by the modified method of Englyst et al., And 0.45 g of porcine pancreatin (P-7545, Sigma Aldrich, St. Louis, Mo., USA) was dispersed in 4 mL of distilled water and centrifuged Min), and 2.7 mL of the supernatant was separated. 0.32 mL of amyloglucosidase (A-7095, Sigma-Aldrich) was mixed with 0.4 mL of distilled water. 0.4 mL of diluted amyloglucosidase was taken and starch degrading enzyme was prepared by mixing 2.7 mL of the previously prepared porcine pancreatin supernatant. To 100 mg of the starch sample, 2 mL of 0.05 M HCl, 0.01 g of pepsin and 10 glass beads (4 mm in diameter) were added and stirred at a constant rate (150 rpm) 1 mL of the prepared starch hydrolyzing enzyme was added and reacted at 37 ° C. The glucose concentration was measured by glucose oxidase and peroxidase assay kits (GAGO-20, Sigma-Aldrich) after mixing with 80% ethanol solution for each hour (0-180 min) The digestible starch content was measured by RDS (rapid digestible starch), the digestible starch content between 20 minutes and 120 minutes by SDS (slowly digestible starch) The content was analyzed by RS (resistant starch).
또한, eGI(expected glycemic index)는 Goni 등의 방법으로 측정하였으며, 구체적으로, 귀리시료와 표준물질(white bread)의 소화율 곡선의 면적 비율로 hydrolysis index(HI)를 계산한 후 Goni 등의 계산식(eGI= 39.71+0.549 HI)으로부터 eGI값을 구하였다.The expected glycemic index (eGI) was measured by the method of Goni et al. Specifically, the hydrolysis index (HI) was calculated as the area ratio of the digestibility curve of the oat sample and the standard bread (white bread) eGI = 39.71 + 0.549 HI).
그 결과 표 10 및 도 3에 나타난 바와 같이, 전분 소화율은 겉귀리가 쌀귀리에 비해 전체적으로 낮았으며 겉귀리의 분쇄크기별 전분의 소화율은 mesh가 증가할수록 소화율이 높게 나타나는 것을 확인하였다(표 10 및 도 3).As a result, as shown in Table 10 and FIG. 3, the starch digestibility was lower than that of rice oats, and the starch digestibility of crust of the crust was higher than that of rice oats ).
반면, eGI 분석값은 겉귀리가 쌀귀리 보다 전체적으로 낮은 경향이었으며, 한편 겉귀리에서는 40 내지 60mesh, 쌀귀리에서는 40 및 100mesh의 경우에서 낮은 값을 보였다(표 10). 식빵(100)을 기준으로 GI를 분석한 결과 쌀귀리(76.57 ~ 84.91)가 겉귀리(80.95 ~ 93.18) 품종에 비해 높은 GI 값을 보였으며, 전반적으로 mesh가 클수록 eGI가 높은 경향을 나타냈고, 쌀귀리의 경우 100 mesh는 80 mesh보다 낮으며 60 mesh와 동일한 수준을 나타내었다.On the other hand, eGI values were lower than those of rice oats, while those of 40 to 60 meshes in the oats and 40 and 100mesh in the oats of rice were low (Table 10). As a result of analysis of GI based on the bread (100), the GI values of rice oats (76.57 ~ 84.91) were higher than those of the oat (80.95 ~ 93.18), and the larger the mesh, the higher the eGI was, In the case of rice oats, 100 mesh was lower than 80 mesh and showed the same level as 60 mesh.
이는 귀리 부침믹스 입도 선정에 관련해 볼 때 믹스 개발 컨셉인 저열량, 저GI화에 부합된다고 판단되어 진다.This is considered to be in accordance with the concept of mix development, low calorie and low GI in relation to selection of grain size of oat drop mix.
(Mesh)smash
(Mesh)
1)RDS(rapidly digetible starch)는 빠르게 소화되는 전분, SDS(slowly digestible starch)는 느리게 소화되는 전분을 나타내며, RS(resistant starch)는 소화되지 않는 전분을 나타낸다. 1) RDS (fast digestible starch) is a fast digestible star; SDS (slowly digestible starch) is a slowly digestible starch; and RS (resistant starch) is an undigested starch.
2)eGI(expected glycemic index)는 예상 혈당지수를 나타냄. 2) eGI (expected glycemic index) indicates the expected blood glucose index.
3)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 3) The different alphabets in the same column mean a significant difference from each other at Duncan's multiple range test at p <0.05.
이에, 상기 <실시예1> 내지 <실시예7>의 결과를 바탕으로, 귀리 부침믹스 개발을 위해, 베타글루칸 함량이 높은 입도크기를 겉귀리 60, 쌀귀리 100 mesh로 선정하였으며, 이를 이용하여 부침믹스 소재로 개발하였다.Based on the results of Examples 1 to 7, for the development of an oatmeal mix, a grain size having a high content of beta-glucan was selected as 60 oat and 100 oat rice oats, It was developed as an infusion mix material.
<실험예 1> 귀리 부침개(전) 제조<Experimental Example 1> Production of oat floss (before)
상기 <실시예1> 내지 <실시예7>의 결과를 바탕으로, 베타글루칸 함량이 높은 입도크기를 갖는 겉귀리(60 mesh) 및 쌀귀리(100 mesh)를 이용하여, 다양한 배합비로 혼합하여 부침개로 제조하였다.(60 mesh) and rice oats (100 mesh) having a high beta-glucan content were mixed at various mixing ratios based on the results of Examples 1 to 7, .
먼저, 쌀가루 100%, 밀가루:귀리(50:50), 쌀가루:귀리(50:50) 및 생귀리 100%로 네가지 군으로 반죽을 만들어 부침개를 제조하였다.First, dough was made into four groups of
그 결과 도 4에 나타난 바와 같이, 귀리가루가 50% 이상 첨가된 부침개의 경우, 표면이 평평하지 않고 울퉁불퉁하며, 겉 테두리가 찢어지는 등 형태 유지가 어려웠다(도 4). 이에, 귀리 부침개 제조 및 품질 검사에서 귀리 함유량을 40% 이내로 배합하여 부침개를 제조하였다.As a result, as shown in Fig. 4, in the case of a flour made of oat flour added in an amount of 50% or more, the surface was not flat and uneven, and it was difficult to maintain the shape such as tearing of the outer surface (Fig. Thus, in the manufacture of oat floss and quality inspection, the content of oats was adjusted to within 40% to prepare a floss.
또한, 귀리 혼합 비율별 부침 믹스의 품질 재현성을 확보하여 품질 특성을 조사하기 위해, 부침개를 하기와 같은 방법으로 제조하였다.In order to ensure the quality reproducibility of the ooze mix according to the oat mixing ratio and to examine the quality characteristics, the bladder was manufactured by the following method.
귀리가루를 혼합한 밀가루 및 쌀가루전은 밀가루, 쌀가루 및 귀리가루의 수분량을 각각 측정한 후 반죽의 수분이 63%가 되도록 물을 추가하여 반죽을 제조하였으며, 지름 8 ㎝, 높이 0.5 ㎝의 원형 스테인레스 스틸틀을 사용하였다. 상기 틀에 5 ㎖의 충분한 기름을 두른 후, 상기 반죽 25 ㎖를 부어 크기와 두께가 균일한 전을 제조하였다. 전 제조 시 온도가 일정하게 유지되는 전자 프라이팬을 이용하여 일정한 온도(150 ℃)에서 5분간 전을 구워내고 10분간 식힌 후 무게를 측정하고, 물성 및 품질 특성 측정을 위한 시료로 사용하였다.The flour and rice flour mixed with oat flour were prepared by measuring the moisture content of wheat flour, rice flour and oat flour, respectively, and adding water to make the dough moisture to 63%. The dough was made into a circular stainless steel having a diameter of 8 ㎝ and a height of 0.5 ㎝ A steel frame was used. The mold was filled with 5 ml of a sufficient amount of oil, and 25 ml of the dough was poured to prepare a uniform size and uniform thickness. The frying pan was heated for 5 minutes at a constant temperature (150 ℃) using an electronic frying pan, which was kept at a constant temperature during the manufacturing process. After cooling for 10 minutes, the weight was measured and used as a sample for measuring physical properties and quality characteristics.
<실험예 2> 귀리 혼합비율별 부침개(전)의 외관 및 색도 분석<Experimental Example 2> Appearance and colorimetric analysis of the clam (front) by oat mixture ratio
상기 <실험예 1>의 방법으로 제조된 귀리 부침개의 외관 및 색도를 조사하였다. The outer appearance and chromaticity of the oat flour made by the method of <Experimental Example 1> were examined.
구체적으로, 색차계(Chroma Meter, CR-300, Minolta Co., Tokyo, Japan)를 이용하여 상기 <실시예 2>와 동일한 방법으로 부침개의 색도를 조사하였다.Specifically, the chromaticity of the decanter was examined in the same manner as in Example 2 using a colorimeter (Chroma Meter, CR-300, Minolta Co., Tokyo, Japan).
그 결과 도 5 및 표 11에 나타난 바와 같이, 귀리 품종에 관계없이 귀리 첨가 비율이 증가할수록 L값은 낮아지고, a값은 증가하는 경향을 나타내었다. 반면, b값은 대조구인 쌀가루전 밀가루전에 비해 유의적으로 낮았으며, 귀리 첨가비율이 증가할수록 b값이 증가하였다.As a result, as shown in FIG. 5 and Table 11, the L value decreased and the a value increased with increasing oat addition rate regardless of the oat varieties. On the other hand, b value was significantly lower than that of whole rice flour before control, and b value increased with increasing oat addition ratio.
(100:0)Flour: Oat
(100: 0)
±0.30d 1.31
± 0.30 d
±0.30d 1.31
± 0.30 d
(90:10)Flour: Oat
(90:10)
±0.25c 1.98
± 0.25 c
±0.19c 2.75
± 0.19 c
(80:20)Flour: Oat
(80:20)
±0.35bc 2.37
± 0.35 bc
±0.28b 3.63
± 0.28 b
(70:30)Flour: Oat
(70:30)
±0.13ab 2.88
± 0.13 ab
±0.07bc 3.50
± 0.07 bc
(60:40)Flour: Oat
(60:40)
±0.04a 3.29
± 0.04 a
±0.93a 5.15
± 0.93 a
(100:0)Rice Flour: Oat
(100: 0)
(90:10)Rice Flour: Oat
(90:10)
±0.63c 2.60
± 0.63 c
±0.37b 1.50
± 0.37 b
(80:20)Rice Flour: Oat
(80:20)
±0.43b 3.92
± 0.43 b
±1.39ab 2.83
± 1.39 ab
±0.32a 7.28
± 0.32 a
±0.87a 4.20
± 0.87 a
(60:40)Rice Flour: Oat
(60:40)
±0.41a 6.42
± 0.41 a
±1.52a 4.32
± 1.52 a
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
<실험예 3> 귀리 혼합비율별 부침개의 흡유율, 조지방 및 수분함량 분석<Experimental Example 3> Analysis of the absorption rate, crude fat and moisture content of the broth according to the mixing ratio of oats
귀리 혼합비율별 부침개의 흡유율, 조지방 및 수분함량을 조사하였다.The absorption rate, crude lipid and moisture content of broiler were investigated by oat mixing ratio.
구체적으로, 수분함량 및 지방함량은 AOAC 방법에 따라 분석하였고, 각 시료를 3회 반복 측정하여 평균값을 %로 나타내었다. 일정량의 반죽으로부터 제조된 전의 무게를 측정하여 반죽과 전의 무게비를 구한 후 흡유율 계산에 사용하였다. 흡유율은 일정량의 반죽이 전을 제조하는 동안 흡수된 기름의 양으로 하기 계산식으로 계산하였다. Specifically, moisture content and fat content were analyzed according to the AOAC method, and each sample was repeated three times and the average value was expressed as%. The weight of the dough and the weight of the dough before and after the dough were measured and used for the absorption rate calculation. The absorption rate was calculated by the following equation, which is the amount of oil absorbed during the production of a certain amount of dough.
흡유율(%) = (전 100g의 지방 × 반죽과 전의 무게비율) - 반죽 100g의 지방Absorption rate (%) = (ratio of weight of 100g fat x paste before) - fat of 100g dough
그 결과 하기 표 12에 나타난 바와 같이, 전의 흡유율은 부침개의 수분함량과 조지방 함량에 따라 다르게 나타났으며, 품종에 관계없이 대부분 조지방 함량이 높은 전에서 흡유율이 높게 나타났고, 수분함량은 40.59~53.46%, 조지방은 4.83~11.27%로 귀리 혼합비율에 따른 뚜렷한 양상이 나타나지 않았다.As a result, as shown in Table 12, the absorption rate of the former was different according to the moisture content and the crude fat content of the blusher, and the absorption rate of the former was higher than that of the latter, regardless of the breed, and the moisture content was 40.59 ~ 53.46% and crude fat 4.83 ~ 11.27%, respectively.
(100:0)Flour: Oat
(100: 0)
±0.25c 6.52
± 0.25 c
±0.22b 6.20
± 0.22 b
±0.22b 6.20
± 0.22 b
(90:10)Flour: Oat
(90:10)
±0.51b 5.22
± 0.51 b
±0.08d 5.31
± 0.08 d
±0.43b 4.83
± 0.43 b
±0.34ab 8.16
± 0.34 ab
(80:20)Flour: Oat
(80:20)
±1.45a 8.23
± 1.45 a
±0.07a 9.88
± 0.07 a
±1.32a 8.21
± 1.32 a
±2.45ab 8.27
± 2.45 ab
(70:30)Flour: Oat
(70:30)
±0.26a 8.40
± 0.26 a
±0.17a 9.98
± 0.17 a
±0.25a 8.94
± 0.25 a
(60:40)Flour: Oat
(60:40)
±0.18a 7.99
± 0.18 a
±0.08b 9.46
± 0.08 b
±0.16a 8.75
± 0.16 a
±0.26ab 7.47
± 0.26 ab
(100:0)Rice Flour: Oat
(100: 0)
±0.62c 7.63
± 0.62 c
±0.62d 7.63
± 0.62 d
±0.52e 5.32
± 0.52 e
±0.52b 5.32
± 0.52 b
±1.5146.25
± 1.51
(90:10)Rice Flour: Oat
(90:10)
±0.41d 7.15
± 0.41 d
±0.02e 6.32
± 0.02 e
±0.34d 6.53
± 0.34 d
±0.67b 7.09
± 0.67 b
±3.3146.68
± 3.31
(80:20)Rice Flour: Oat
(80:20)
±0.30b 7.69
± 0.30 b
±0.05c 7.92
± 0.05 c
±0.25b 7.80
± 0.25 b
±0.99b 7.63
± 0.99 b
±1.2547.81
± 1.25
±0.32a 8.22
± 0.32 a
±0.07a 8.90
± 0.07 a
±0.20a 8.85
± 0.20 a
±0.1345.62
± 0.13
(60:40)Rice Flour: Oat
(60:40)
±0.42e 6.88
± 0.42 e
±0.01b 8.50
± 0.01 b
±0.36c 7.43
± 0.36 c
±3.1547.86
± 3.15
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
<실험예 4> 귀리 혼합비율별 부침개의 물성 분석<Experimental Example 4> Analysis of physical properties of the humidifier by the mixing ratio of oats
귀리 혼합비율에 따른 부침개(전)의 물성을 확인하였다.The physical properties of the broccoli (before) according to the mixing ratio of oats were confirmed.
구체적으로, 부침개(전)의 물성은 Texture analyser(TA-XT.plus, Stable Micro Systems Co. Ltd., London, England)을 이용하여 측정하였다.Specifically, the physical properties of the blusher were measured using a texture analyzer (TA-XT.plus, Stable Micro Systems Co. Ltd., London, England).
그 결과 하기 표 13에 나타난 바와 같이, 쌀귀리를 이용한 밀가루전은 응집성과 점착성이 감소하는 경향을 나타내었으며, 겉귀리의 경우 쌀가루전은 귀리 첨가비율이 증가할수록 응집성, 접착성은 유의적으로 감소한 반면, 밀가루전은 유의적 차이를 나타내지 않았다(표 13).As a result, as shown in Table 13, the cohesion and adhesiveness of the wheat flour using rice oats tended to decrease, while the cohesiveness and adhesiveness of the oat flour before rice flour increased significantly , And wheat flour showed no significant difference (Table 13).
또한, 대조구인 밀가루전은 쌀가루전에 비해 경도, 탄력성, 씹힘성 등 모든 물성특성이 높게 나타났으며, 품종에 관계없이 밀가루와 귀리의 혼합전이 쌀가루를 혼합한 전에 비해 일부 경도를 제외한 탄력성, 응집성, 점착성 등이 모두 높게 나타남을 확인하였다(표 13).In addition, all the properties such as hardness, elasticity and chewiness were higher in the control flour before the rice flour than in the case of rice flour before the mixing of flour and oat regardless of the varieties. (Table 13).
혼합비율(%)Oat type
Mixing ratio (%)
(90 : 10)Flour: Rice Oat
(90:10)
(80 : 20)Flour: Rice Oat
(80:20)
(70 : 30)Flour: Rice Oat
(70:30)
(60 : 40)Flour: Rice Oat
(60:40)
(90 : 10)Rice Flour: Rice Oat
(90:10)
(80 : 20)Rice Flour: Rice Oat
(80:20)
(70 : 30)Rice Flour: Rice Oat
(70:30)
(60 : 40)Rice Flour: Rice Oat
(60:40)
(90 : 10)Wheat flour: oat
(90:10)
(80 : 20)Wheat flour: oat
(80:20)
(70 : 30)Wheat flour: oat
(70:30)
(60 : 40)Wheat flour: oat
(60:40)
(90 : 10)Rice flour: Oatmeal
(90:10)
(80 : 20)Rice flour: Oatmeal
(80:20)
(70 : 30)Rice flour: Oatmeal
(70:30)
(60 : 40)Rice flour: Oatmeal
(60:40)
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
<실험예 5> 귀리 혼합비율별 부침개의 베타글루칸 및 총전분 함량 분석 <Experimental Example 5> Analysis of Beta Glucan and Total Starch Contents of Broccoli by Oat Mixing Ratio
귀리 혼합비율별 부침개의 베타글루칸 및 총전분 함량을 분석하였다.The content of beta - glucan and total starch in the broiler was analyzed by oat mixing ratio.
구체적으로, 베타글루칸 함량은 상기 <실시예 4>와 동일한 방법으로 Megazyme kit(K-BGLU)를 사용하여 측정하였으며, 총전분 함량은 상기 <실시예 6>과 같은 방법으로 Megazyme kit(K-TSTA)사용하여 측정하였다.Specifically, the content of beta-glucan was measured using a Megazyme kit (K-BGLU) in the same manner as in Example 4, and the total starch content was measured using a Megazyme kit (K-TSTA ).
귀리를 혼합하여 제조한 밀가루전과 쌀가루전의 베타글루칸 함량을 측정한 결과, 표 14에 나타난 바와 같이 귀리의 혼합비율이 증가함에 따라 베타글루칸 함량이 증가함을 알 수 있었으며, 품종에 따른 함량 차이는 나타나지 않았다(표 14).As shown in Table 14, the betaglucan content before and after the addition of oats was found to increase with increasing oat mixing ratio. The content of betaglucan before rice flour was increased, (Table 14).
따라서 부침믹스의 선정 기준인 베타클루칸 함량으로 볼 때 곡물가루와 귀리가루의 적정혼합비는 40%가 적정한 것으로 판단되었다.Therefore, it was concluded that 40% of the optimum mixing ratio of cereal flour and oat flour is appropriate in terms of the content of betaclucan, which is the selection criterion of the infusion mix.
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
또한, 귀리가루 혼합비별 전의 총전분 함량을 확인한 결과, 하기 표 15에 나타난 바와 같이, 밀가루전은 쌀가루전에 비해 TS 함량이 전반적으로 낮았으며 품종에 상관없이 귀리 첨가비율이 증가할수록 함량이 낮아짐을 알 수 있었다(표 15).As shown in Table 15 below, the total amount of starch before the addition of the oat flour was lower than that before the rice flour. As shown in Table 15, the content of TS was lower than that before the rice flour, (Table 15).
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
<실험예 6> 귀리 혼합비율별 부침개의 호화특성 분석 EXPERIMENTAL EXAMPLE 6 Analysis of Gelatinization Characteristic of Broccoli by Oat Mixing Ratio
귀리 혼합비율별 부침개의 호화특성을 분석하였다.This study was carried out to analyze the gelatinization characteristics of broiler by oat mixing ratio.
구체적으로, 실험 방법은 <실시예 6>과 동일한 방법으로 수행하였다.Specifically, the experimental method was performed in the same manner as in Example 6.
그 결과 하기 표 16에 나타난 바와 같이, 품종에 관계없이 최고점도, 최소점도, 강하점도, 최종점도 모두 밀가루전에 비해 쌀가루전이 높게 나타났으며, 쌀귀리를 첨가한 밀가루전의 강하점도, 최종점도, 치반점도는 쌀가루전과는 반대로 귀리첨가비율이 증가할수록 높게 나타났으며 호화개시온도에 따른 유의적인 차이는 나타나지 않았다(표 14).As a result, as shown in Table 16, rice flour transition was higher than that of wheat flour in all of the highest, lowest, lowest, and final viscosity irrespective of the variety, and the lowering viscosity, final viscosity, The degree of discoloration was higher with the addition of oats than with rice flour, and there was no significant difference according to the initiation temperature (Table 14).
반면, 겉귀리는 밀가루전의 경우 귀리 첨가비율에 따른 호화개시온도의 유의적 차이가 없었으며, 쌀가루전은 귀리가 첨가됨에 따라 높아짐을 알 수 있었다(표 14). 아울러 쌀귀리와 마찬가지로 밀가루전과 쌀가루전의 최고점도, 최소점도, 강하점도, 최종점도는 귀리 첨가비율에 따른 경향이 반대로 나타났다.On the other hand, there was no significant difference in the onset temperature of oat flour according to the addition rate of oats, and that of rice flour increased with the addition of oats (Table 14). In addition, as in rice oats, the maximum viscosity, minimum viscosity, drop viscosity, and final viscosity before and after rice flour were inversely related to the addition of oats.
혼합비율(%)Oat varieties
Mixing ratio (%)
(RVU)Peak viscosity
(RVU)
(RVU)Trough
(RVU)
(RVU)Breakdown
(RVU)
(RVU)Final Viscosity
(RVU)
(RVU)Setback
(RVU)
(min)Peak Time
(min)
(℃)Pasting Temp
(° C)
(100:0)Flour: Rice Oat
(100: 0)
(90:10)Flour: Rice Oat
(90:10)
(80:20)Flour: Rice Oat
(80:20)
(70:30)Flour: Rice Oat
(70:30)
(60:40)Flour: Rice Oat
(60:40)
(100:0)Rice Flour: Rice Oat
(100: 0)
(90:10)Rice Flour: Rice Oat
(90:10)
(80:20)Rice Flour: Rice Oat
(80:20)
(70:30)Rice Flour: Rice Oat
(70:30)
(60:40)Rice Flour: Rice Oat
(60:40)
(90:10)Wheat flour: oat
(90:10)
(80:20)Wheat flour: oat
(80:20)
(70:30)Wheat flour: oat
(70:30)
(60:40)Wheat flour: oat
(60:40)
(90:10)Rice flour: Oatmeal
(90:10)
(80:20)Rice flour: Oatmeal
(80:20)
(70:30)Rice flour: Oatmeal
(70:30)
(60:40)Rice flour: Oatmeal
(60:40)
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
<실험예 7> 귀리 혼합비율별 부침개의 전분 소화율 및 eGI 분석 <Experimental Example 7> Starch digestibility and eGI analysis of the beef broiler according to oat mixing ratio
곡물가루 대체 귀리가루 혼합비율별 부침개의 전분 소화율 및 eGI를 분석하였다. The starch digestibility and eGI of corn flour were analyzed by oat flour mixing ratio.
구체적인 실험 방법은 상기 <실시예 7>과 동일한 방법으로 수행하였다.A specific experimental method was carried out in the same manner as in <Example 7>.
그 결과 하기 표 17에 나타난 바와 같이, 쌀귀리의 RDS 및 SDS는 각각 1.22~5.88%, 19.89~32.46%로 귀리 혼합비율에 따른 함량 차이는 없었으나, RS는 혼합비율 증가에 따라 유의적으로 증가하였다(표 17).As shown in Table 17, RDS and SDS of the oats of rice were 1.22 ~ 5.88% and 19.89 ~ 32.46%, respectively, but there was no significant difference according to the mixing ratio of oats, (Table 17).
또한, 겉귀리의 RS는 밀가루전과 쌀가루전 모두 귀리첨가 비율에 따라 증가하였으며, RDS와 SDS는 첨가비율에 따른 밀가루전과 쌀가루전의 함량이 각각 다른 경향을 나타내었다. 즉 귀리 첨가비율이 증가됨에 따라 밀가루전의 RDS와 SDS는 각각 감소, 증가한 반면 쌀가루전의 RDS 및 SDS는 증가, 감소하였음을 알 수 있다. In addition, the RS of crust increased with the addition of oats to both wheat flour and rice flour. RDS and SDS showed different contents of wheat flour before rice flour and before rice flour, respectively. That is, RDS and SDS before wheat flour decreased and increased with increasing oat addition ratio, respectively, whereas RDS and SDS before rice flour increased and decreased, respectively.
또한, 곡물(밀, 쌀)가루 대체 귀리 40% 혼합비율의 처리구에서 SDS값은 밀가루 베이스와 쌀가루 베이스일 때 서로 상반된 결과를 보이는 것을 확인하였다.It was also confirmed that the SDS values of the wheat and rice flour substitutes were comparable to those of the wheat flour and rice flour base at the 40% oat mixture ratio.
(100:0)Flour: Oat
(100: 0)
(90:10)Flour: Oat
(90:10)
±1.76ab 3.00
± 1.76 ab
(80:20)Flour: Oat
(80:20)
±0.19b 1.46
± 0.19 b
(70:30)Flour: Oat
(70:30)
±0.96b 1.22
± 0.96 b
(60:40)Flour: Oat
(60:40)
(100:0)Rice Flour: Oat
(100: 0)
±0.46b 1.62
± 0.46 b
(90:10)Rice Flour: Oat
(90:10)
(80:20)Rice Flour: Oat
(80:20)
±2.96a 7.20
± 2.96 a
(70:30)Rice Flour: Oat
(70:30)
(60:40)Rice Flour: Oat
(60:40)
1)RDS(rapidly digetible starch)는 빠르게 소화되는 전분, 2)SDS(slowly digestible starch)는 느리게 소화되는 전분을 나타내며, 3)RS(resistant starch)는 소화되지 않는 전분을 나타낸다. 1) Rapid digestible starch (RDS) is rapidly digested starch; 2) SDS (slowly digestible starch) is slowly digested starch; and 3) RS (resistant starch) is non-digestible starch.
4)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 4) The different alphabets in the same column mean a significant difference from each other at Duncan's multiple range test at p <0.05.
또한, 하기 표 18에 나타난 바와 같이, 전분소화율(eGI)은 품종에 관계없이 귀리 첨가비율이 증가함에 따라 감소하는 경향을 나타내었으며, 이는 귀리의 베타글루칸의 함량과 상관성이 있을 것으로 판단된다. 또한, 밀가루전이 쌀가루전에 비해 다소 낮은 eGI 수치를 나타내었다(표 18).As shown in the following Table 18, the starch digestibility (eGI) tended to decrease with increasing oat addition rate regardless of the type of the starch, and it was considered that the starch digestibility (eGI) was correlated with the content of betaglucan in oats. Also, the flour transfer showed a somewhat lower eGI value compared to before rice flour (Table 18).
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
곡물첨가 귀리부침가루 믹스의 적정 곡물 선정을 위해 eGI 값을 살펴 본 결과, 밀가루 베이스일 때는 쌀귀리 부침믹스가, 쌀가루 베이스일 때는 겉귀리 부침믹스에서 eGI값이 낮게 나타났다. 따라서 곡물첨가 귀리부침가루 믹스의 적정 곡물을 선정하고자 할 때 밀가루 베이스일 경우는 쌀귀리를, 쌀가루 베이스 일는 겉귀리를 이용하는 것이 혈당지수(GI)가 낮은 기능성 부침믹스 개발에 효과적이다.The eGI value for the grain selection of cereal added oat flour mix was lower than that of rice oat flour mix for wheat flour base and for oat flour mix for rice flour base. Therefore, it is effective to develop functional impregnated mix with low blood glucose index (GI) by using rice oat for flour base and rice oat for rice flour base when trying to select the proper grain of grain added oat flour mixture.
<실험예 8> 귀리 혼합비율별 부침개의 기호도 검사<Experimental Example 8> Examination of the preference degree of the broiler according to the mixing ratio of oats
마지막으로, 귀리 혼합비율별 부침개의 외관, 풍미, 맛에 대한 기호도를 조사하였다.Finally, preference for appearance, flavor, and taste of broiler was investigated by oat mixing ratio.
구체적으로, 농촌진흥청에 근무하는 25-37세 여성 15명을 대상으로 실험목적 및 평가항목들에 대해 설명한 후 관능검사를 실시하였다. 각 시료는 만든지 10분 경과 후, 일정한 크기로 잘라 흰색의 동일한 접시에 담아 동시에 제공하였으며 평가 사이에 입을 헹굴 수 있도록 정수한 물과 함께 제시하였다. 평가항목은 색(color), 향(flavor), 맛(taste), 조직감(texture), 전반적 기호도(overall preperence)에 관한 특성을 9점 척도로 평가하였다. Specifically, 15 women aged 25 to 37 who worked at the Rural Development Administration conducted the sensory evaluation after explaining the purpose and evaluation items of the experiment. Each sample was cut at a constant size and placed on the same white plate at the same time after 10 minutes of being made, and was presented with purified water so that the mouth could be rinsed between evaluations. The evaluation items were evaluated on a 9 point scale for color, flavor, taste, texture, and overall preperence.
그 결과 하기 표 19에 나타난 바와 같이, 귀리 품종에 상관없이 대조구인 밀가루전과 쌀가루전에 비해 외관 및 풍미, 전반적 기호도가 높았으며 쌀귀리전의 관능평가 결과 외관, 풍미, 맛에 대한 기호도는 밀가루전에 비해 쌀가루전이 높았으나 유의적인 차이는 없었다(표 19).As a result, as shown in Table 19, the appearance, flavor and overall acceptability were higher than before the control flour and rice flour regardless of the oat varieties, and the sensory evaluation before rice oats was favorable for the appearance, flavor and taste, There was no significant difference between the two groups (Table 19).
반면, 겉귀리는 귀리가 첨가됨에 따라 밀가루전의 풍미와 쌀가루전의 외관이 유의적으로 증가하였으며, 전반적으로 귀리가 첨가됨에 따라 기호도가 다소 높아지는 것을 알 수 있었다.On the other hand, the addition of oats to the crust of oat increased the pre - flour flavor and appearance before rice flour, and it was found that the degree of preference increased slightly with the addition of oats.
따라서 곡물가루 대비 귀리첨가에 따라 전반적 기호도가 좋아지는 처리구는 쌀가루 첨가대비 겉귀리 40%가 가장 좋았고, 쌀귀리 10~40%간에는 유의적인 차이가 없었다. Therefore, the treatments which showed better overall acceptability by adding oats to grain flour were 40% and 40%, respectively, compared with rice flour.
이에, 귀리첨가 믹스의 적정 배합비는 함량이 높은 40%가 적정할 것으로 판단하였으며, 쌀가루에서도 같은 경향이 나타났다.Therefore, the optimum mixing ratio of oat - added mix was 40%, which was high, and the same tendency also appeared in rice flour.
±2.194.73
± 2.19
±1.244.60
± 1.24
±1.735.47
± 1.73
±1.50b 4.33
± 1.50 b
(90:10)Flour: Oat
(90:10)
±1.615.80
± 1.61
±1.775.00
± 1.77
±1.37ab 5.20
± 1.37 ab
±1.965.40
± 1.96
±1.22ab 5.27
± 1.22 ab
(80:20)Flour: Oat
(80:20)
±1.635.33
± 1.63
±1.634.33
± 1.63
±1.00ab 5.00
± 1.00 ab
±1.715.27
± 1.71
±1.44ab 5.07
± 1.44 ab
(70:30)Flour: Oat
(70:30)
±1.925.87
± 1.92
±2.024.33
± 2.02
±1.96ab 4.87
± 1.96 ab
±2.075.53
± 2.07
±1.80ab 5.40
± 1.80 ab
(60:40)Flour: Oat
(60:40)
±1.445.27
± 1.44
±2.074.00
± 2.07
±1.72a 5.60
± 1.72 a
±1.595.60
± 1.59
±1.64a 5.60
± 1.64 a
±1.64c 3.40
± 1.64 c
±1.824.80
± 1.82
±1.524.20
± 1.52
±1.88b 4.47
± 1.88 b
±1.15b 3.80
± 1.15 b
(90:10)Rice Flour: Oat
(90:10)
±1.76b 4.67
± 1.76 b
±1.534.93
± 1.53
±1.194.87
± 1.19
±1.49a 5.73
± 1.49 a
±1.39a 5.07
± 1.39 a
(80:20)Rice Flour: Oat
(80:20)
±1.91ab 5.73
± 1.91 ab
±1.195.47
± 1.19
±1.015.20
± 1.01
±1.30a 5.87
± 1.30 a
±1.21a 5.80
± 1.21 a
(70:30)Rice Flour: Oat
(70:30)
±1.33a 6.27
± 1.33 a
±1.595.60
± 1.59
±1.495.27
± 1.49
±1.28a 5.93
± 1.28 a
±1.52a 5.80
± 1.52 a
(60:40)Rice Flour: Oat
(60:40)
±1.55ab 5.53
± 1.55 ab
±2.094.93
± 2.09
±2.025.33
± 2.02
±1.58a 6.27
± 1.58 a
±1.96a 5.47
± 1.96 a
±1.594.67
± 1.59
±1.23b 4.33
± 1.23 b
±1.254.13
± 1.25
±1.415.53
± 1.41
±1.594.60
± 1.59
(90:10)Flour: Oat
(90:10)
±1.674.93
± 1.67
±1.35b 4.67
± 1.35 b
±1.704.80
± 1.70
±1.625.27
± 1.62
±1.225.27
± 1.22
(80:20)Flour: Oat
(80:20)
±1.795.07
± 1.79
±1.67ab 5.07
± 1.67 ab
±1.495.27
± 1.49
±1.395.73
± 1.39
±1.585.27
± 1.58
(70:30)Flour: Oat
(70:30)
±1.905.80
± 1.90
±1.78ab 4.80
± 1.78 ab
±1.534.73
± 1.53
±1.645.13
± 1.64
±1.595.40
± 1.59
(60:40)Flour: Oat
(60:40)
±1.575.80
± 1.57
±1.93a 6.00
± 1.93 a
±1.475.20
± 1.47
±1.405.40
± 1.40
±1.595.40
± 1.59
±1.86c 3.80
± 1.86 c
±1.904.80
± 1.90
±1.31b 4.00
± 1.31 b
±1.764.60
± 1.76
±1.33b 4.07
± 1.33 b
(90:10)Rice Flour: Oat
(90:10)
±1.64bc 4.40
± 1.64 bc
±1.634.67
± 1.63
±1.28ab 4.73
± 1.28 ab
±1.465.47
± 1.46
±1.68ab 5.13
± 1.68 ab
(80:20)Rice Flour: Oat
(80:20)
±1.76ab 5.33
± 1.76 ab
±1.405.40
± 1.40
±1.51a 5.47
± 1.51 a
±1.725.60
± 1.72
±1.95ab 5.33
± 1.95 ab
(70:30)Rice Flour: Oat
(70:30)
±1.62a 5.93
± 1.62 a
±1.445.07
± 1.44
±1.80ab 4.60
± 1.80 ab
±1.844.40
± 1.84
±1.52ab 4.80
± 1.52 ab
(60:40)Rice Flour: Oat
(60:40)
±1.70a 6.20
± 1.70 a
±1.665.20
± 1.66
±1.25a 5.47
± 1.25 a
±1.504.67
± 1.50
±1.46a 5.47
± 1.46 a
1)같은 열에 위치한 서로 다른 알파벳은 Duncan's multiple range test에 의하여 p<0.05에서 서로 간의 유의성있는 차이를 의미한다. 1) The different alphabets located in the same column indicate a significant difference between each other at Duncan's multiple range test at p <0.05.
Claims (5)
상기 혼합은 귀리 첨가 비율이 10 내지 40 중량%인 부침믹스 제조방법.
A method for producing an infusion mix wherein the content of betaglucan prepared by mixing rice flour or wheat into oats of 50 to 70 mesh or oats of 90 to 110 mesh is enhanced,
Wherein the mixing is carried out in an amount of 10 to 40% by weight of oats.
The method of claim 1, wherein the infusion mix has a low glycemic index (GI) or a low starch digestibility.
[Claim 2] The method of claim 1, wherein the infusion mix is prepared by mixing rice oats with wheat flour and oats with rice flour.
6. An oatmeal mix according to claim 1, wherein the content of betaglucan is increased.
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KR20120020160A (en) * | 2009-05-18 | 2012-03-07 | 다우 글로벌 테크놀로지스 엘엘씨 | Methods for reducing oil uptake of fried foods |
KR20130088364A (en) * | 2012-01-31 | 2013-08-08 | 김은정 | A METHOD OF MANUFACTURING SOYBEAN PASTE CONTAINING β-GLUCAN AND THE SOYBEAN PASTE BY THE METHOD |
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KR20120020160A (en) * | 2009-05-18 | 2012-03-07 | 다우 글로벌 테크놀로지스 엘엘씨 | Methods for reducing oil uptake of fried foods |
KR20130088364A (en) * | 2012-01-31 | 2013-08-08 | 김은정 | A METHOD OF MANUFACTURING SOYBEAN PASTE CONTAINING β-GLUCAN AND THE SOYBEAN PASTE BY THE METHOD |
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Title |
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이유영 외 12명. 국내 육성 귀리 품종의 이화학적 특성 및 식이섬유 함량 분포. Korean J. Breed. Sci. : 2016년 3월, 48(1) : pp. 37~47. 1부. * |
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