KR20180052908A - Device for continuous measuring pO2 and pCO2 of aquarium without biofouling on the sensors using air-water equilibrium - Google Patents

Abstract

The present invention relates to an apparatus for continuously measuring oxygen and carbon dioxide in a fish farm without biological contamination caused by a sensor, which comprises: a floating member some part of which is submerged in seawater where marine microbes live, and has a head space inside thereof; a bubble introduction pipe which is submerged in seawater to communicate with the lower part of the floating member; a dehumidifier one side of which communicates with the upper part of the floating member to dehumidify the air provided by the floating member; a first circulation pipe which circulates the air discharged by the dehumidifier to introduce the same into the floating member; a second circulation pipe which circulates the air discharged by the dehumidifier to introduce the same into the floating member; a carbon dioxide partial pressure measurement unit which measures partial pressure of carbon dioxide within the air introduced into the first circulation pipe; and an oxygen partial pressure measurement unit which measures partial pressure of oxygen within the air introduced into the second circulation pipe.

Description

[0001] The present invention relates to a device for continuous measurement of oxygen and carbon dioxide in a farm without biological contamination of the sensor,

An embodiment of the present invention relates to an apparatus for continuously measuring oxygen and carbon dioxide in a farm without biological contamination of the sensor.

In general, it is widely used as an indicator of the degree of acidity of seawater in order to study the effect of carbon dioxide partial pressure in aquaculture tank on marine habitat environment.

Generally, in order to measure the partial pressure of carbon dioxide in a water tank in which marine microorganisms are inhabited, a part of seawater in a water tank is continuously sampled and sampled seawater is injected into a closed measuring chamber through a high pressure pump to measure carbon dioxide partial pressure in seawater and carbon dioxide The equilibrium of the partial pressures is detected and the partial pressure of carbon dioxide in the seawater is measured.

However, when sampling a part of seawater in the tank and injecting high pressure into the measurement chamber through a high-pressure pump to measure the partial pressure of carbon dioxide in the seawater, micro-marine organisms in the tank enter the measurement chamber, It is impossible to apply it to the observation and culture of marine life because it is dead.

In order to solve such a problem, Korean Patent Registration No. 10-1363012 discloses a method for continuously monitoring the partial pressure of carbon dioxide in seawater in a marine aquarium, wherein the partial pressure of carbon dioxide We proposed a measuring device.

However, the apparatus for measuring partial pressure of carbon dioxide in seawater proposed in the above-mentioned Korean patent has a configuration in which a measuring device for measuring the partial pressure of carbon dioxide and an air pump for air circulation are arranged in series to supply the sampled air pressure supplied to the measuring device and air The amount of air to be sampled from the isolation space is not uniform and the pressure adjustment pipe is directly installed on the floating member. As a result, clogging occurred due to moisture contained in the air or fine droplets generated by the bubbles in the isolation space formed inside the floating member, so that the pressure of the air supplied to the measuring device is not uniform, Do it exactly on the meter The difficult to measure the carbon dioxide partial pressure, measurement of the oxygen partial pressure was not at all.

Dissolved oxygen is measured by an electrochemical sensor (galvanic sensor), optoelectronic sensor (optode), chemical titration method, etc. for water quality management in a water tank. Compared to the chemical titration method, which can measure intermittently, it is possible to measure continuously for long time when it is put into water with dissolved oxygen measurement sensor. However, when oxygen and carbon dioxide measurement sensor in water is operated in a long time underwater environment, And there was a problem that measurement could not be performed in the long term.

The present invention has been devised to solve the above-mentioned problem, and it is intended to enable continuous measurement of oxygen and carbon dioxide, which are indicators of respiration rate of living organisms, without bio-pollution by analyzing gaseous gas in the atmosphere and underwater environment.

The present invention relates to a method and apparatus for measuring the equilibrium oxygen and carbon dioxide partial pressures between two mediums by continuously contacting the culture medium of the cultured aquaculture and the gas of the non-circulating circulating medium to form an equilibrium between oxygen and carbon dioxide partial pressure We intend to be able to evaluate the quality of aquaculture using the measured oxygen and carbon dioxide partial pressures after continuously extracting the gas of the measurement medium.

In addition, the present invention makes it possible to simultaneously measure changes in oxygen and carbon dioxide, which are representative respiration indexes of marine life, and to increase the sensitivity of respiration rate measurement using the change in partial pressure of carbon dioxide compared to the dissolved oxygen method, We want to make long-term continuous measurement of oxygen and carbon dioxide partial pressure in water.

In addition, the present invention enables simultaneous measurement of oxygen and carbon dioxide to analyze the energy metabolism state of living organisms, and enables measurement of respiration rate according to the life history changes of living organisms moving in both directions from the atmosphere to seawater or freshwater.

The apparatus for continuously measuring oxygen and carbon dioxide in a farm without biological contamination of the sensor according to the present embodiment for solving the above-mentioned problems is characterized in that a head space is formed inside the micro- A floating member; A bubble introduction pipe that is submerged in the seawater and communicates with a lower portion of the floating member; A dehumidifier that communicates with one side of the upper portion of the floating member to dehumidify air provided from the floating member; A first circulation pipe through which air flowing out through the dehumidifier is circulated and flows into the floating member; A second circulation pipe through which air flowing out through the dehumidifier is circulated and flows into the floating member; A carbon dioxide partial pressure measuring device for measuring a partial pressure of carbon dioxide in the air provided into the first circulation pipe; And an oxygen partial pressure measuring device for measuring a partial pressure of oxygen in the air provided into the second circulation pipe.

According to another embodiment of the present invention, a first circulation pump installed on the first circulation pipe for introducing the air flowing out of the dehumidifier into the carbon dioxide partial pressure measuring instrument; And a second circulation pump installed on the second circulation pipe for introducing the air discharged from the dehumidifier into the oxygen partial pressure measuring device.

According to another embodiment of the present invention, there is provided an air conditioning system comprising: an air circulation pipe through which air flows through a pressure control pipe; And a bubble generator coupled to an end of the air circulation pipe and disposed in the bubble introduction pipe.

According to another embodiment of the present invention, there is provided a bubble introduction pipe pump arranged in the bubble introduction pipe at an upper portion of the bubble generator for supplying seawater containing bubbles generated in the bubble generator into the floating member .

According to another embodiment of the present invention, there is provided a water treatment system comprising: a temperature sensor installed on the floating member to measure a temperature of seawater in the floating member; And a salinity sensor installed on the floating member and measuring the salinity of the seawater in the floating member.

According to another embodiment of the present invention, there is further provided a water tank in which the seawater is received, and the floating member may be configured to float in the water tank.

According to the embodiment of the present invention, since the gaseous gas is analyzed in the atmosphere and underwater environment, it is possible to continuously measure oxygen and carbon dioxide, which are indicators of respiration rate of living organisms, without biological pollution.

According to the embodiment of the present invention, the oxygen and the carbon dioxide partial pressure are equilibrated between the two mediums by continuously contacting the culture medium of the measurement target culture plant with the gas of the non-circulating circulation measurement medium, and the oxygen concentration and the carbon dioxide partial pressure The equilibrium measurement media can be continuously extracted and the oxygen and carbon dioxide partial pressures can be used to evaluate the water quality of the farm.

In addition, according to the embodiment of the present invention, it is possible to simultaneously measure changes in oxygen and carbon dioxide, which are representative respiration indexes of marine organisms, and the sensitivity of respiration rate measurement using the change of the partial pressure of carbon dioxide relative to the dissolved oxygen method is improved by several times, Continuous long-term measurement of oxygen and carbon dioxide partial pressure in water without contamination is possible.

In addition, according to the embodiment of the present invention, it is possible to analyze the energy metabolism state of a living organism by measuring oxygen and carbon dioxide at the same time, and it is possible to measure the respiration rate according to the life history change of living organisms moving from the atmosphere to sea water or fresh water.

FIG. 1 is a view showing an apparatus for continuously measuring oxygen and carbon dioxide in a farm without biological contamination of a sensor according to an embodiment of the present invention.
FIG. 2 is a view showing an apparatus for continuously measuring oxygen and carbon dioxide in a farm without biological contamination of a sensor according to another embodiment of the present invention.
FIGS. 3 and 4 are graphs for explaining a method for continuously measuring oxygen and carbon dioxide in a farm without biological contamination of the sensor.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description, well-known functions or constructions are not described in detail to avoid unnecessarily obscuring the subject matter of the present invention. In addition, the size of each component in the drawings may be exaggerated for the sake of explanation and does not mean a size actually applied.

The biological habitat environment of the farm changes rapidly due to the density and development stage of the organism, the feeding rate of feed, the decay of the remaining feed, the activity of microorganism, temperature, and excretion. In order to manage the environment of these farms in a stable manner, it is necessary to continuously measure changes in oxygen and carbon dioxide, which are indispensable indicators of water quality management, at the same time.

The device for continuous measurement of oxygen and carbon dioxide in the farm without biological contamination of the sensor according to the present invention can simultaneously measure oxygen and carbon dioxide in the culture water of the farm without biological contamination at the same time. In order to calculate two kinds of water quality indexes, the seawater is continuously contacted with the endless circulating gas in the floating member to make the equilibrium between the seawater and the air, and the part of the equilibrated gas is extracted to obtain the equilibrium state between the seawater and the air After the oxygen and carbon dioxide partial pressures are measured, the measured air (gas) is injected again into the floating member. At this time, the oxygen and carbon dioxide measurement sensor does not come into direct contact with water, so there is no contamination by aquatic organisms.

FIG. 1 is a view showing an apparatus for continuously measuring oxygen and carbon dioxide in a farm without biological contamination of a sensor according to an embodiment of the present invention.

Hereinafter, an apparatus for continuously measuring oxygen and carbon dioxide in a farm environment without biological contamination of a sensor according to an embodiment of the present invention will be described with reference to FIG.

As shown in FIG. 1, an apparatus for continuously measuring oxygen and carbon dioxide in a farm without biological contamination of a sensor according to an embodiment of the present invention includes a floating member 110, a bubble introduction pipe 120, a dehumidifier 130, A first circulation pipe 140, a second circulation pipe 150, a carbon dioxide partial pressure measuring device 145 and an oxygen partial pressure measuring device 155.

The floating member 110 is partially submerged in the seawater 161 in which micro-marine organisms live, and a head space is formed therein.

More specifically, the floating member 110 floats on the seawater 161 and partially floats on the float member 110. The floating member 110 has a head space 111, that is, An isolation space is formed and air is introduced into the head space 111. Therefore, the floating member 110 can prevent the water level fluctuation of the balancer between the liquid (seawater) and the gas (air) according to the water level change. The head space 111 can recover the gas (air) And the air (air).

A bubble introducing pipe 120 is connected to the lower portion of the floating member 110. That is, the bubble introduction pipe 120 may be configured to be submerged in seawater and communicate with the lower portion of the floating member 110.

A bubble generator 175 may be disposed in the bubble introduction pipe 120. The bubble generator 175 is coupled to the end of the air circulation pipe 170 and the air circulation pipe 170 can be supplied with air through the pressure control pipe 171.

Therefore, the air introduced from the pressure control pipe 171 through the air circulation pipe 170 can generate bubble-shaped air in the bubble introduction pipe 120 through the bubble generator 175.

The bubble-shaped air thus generated flows into the head space 111 of the floating member 110 and circulates through the dehumidifier 130 to the first circulation pipe 140 and the second circulation pipe 150.

More specifically, the dehumidifier 130 communicates with one side of the upper portion of the floating member 110 to dehumidify air supplied from the floating member 110, and the dehumidified air flows through the first circulation pipe 140, And the second circulation pipe (150).

At this time, a carbon dioxide partial pressure measuring instrument 145 is installed in the first circulation pipe 140, and an oxygen partial pressure measuring instrument 155 is installed in the second circulation pipe 150.

Therefore, the air introduced from the dehumidifier 130 into the first circulation pipe 140 can measure the partial pressure of carbon dioxide by the carbon dioxide partial pressure meter 145, and the second circulation pipe 150 The air can measure the oxygen partial pressure by the oxygen partial pressure measuring instrument 155.

In this case, a pump 141 may be installed in the first circulation pipe 140 so as to more effectively introduce the air flowing out of the dehumidifier 130 into the carbon dioxide partial pressure measuring device 145, A pump 151 may be installed in the second circulation pipe 150 so as to more effectively introduce the outflowed air into the oxygen partial pressure measuring device 155.

Meanwhile, the dehumidifier 130 may be filled with a dehumidifying agent composed of a porous material in order to remove moisture present in the air. The dehumidifying agent may be a porous moisture absorbent such as a silica gel or an active bath.

The air circulated through the first circulation pipe 140 and the second circulation pipe 150 flows into the head space 111 of the floating member 110 again.

Therefore, according to the apparatus for continuously measuring oxygen and carbon dioxide in a farm environment without biological contamination of the sensor according to an embodiment of the present invention, the air is circulated from the air circulation pipe 170 through the bubble generator 175 into the bubble introduction pipe 120 The buoyancy of the introduced air raises the seawater in the bubble introduction pipe 120 and the air and the seawater continuously contact to reach the gas equilibrium state between the seawater and the air. At this time, the surrounding seawater is infinitely supplied and the air is continuously circulated in the closed space, and a part of the circulated air is extracted and the carbon dioxide and oxygen are measured simultaneously through the carbon dioxide partial pressure measuring instrument 145 and the oxygen partial pressure measuring instrument 155 can do. Thus, the measured gas is continuously reused, and the measured oxygen partial pressure of the gaseous phase can be estimated using the equilibrium coefficient between oxygen and liquid.

The temperature sensor 181 may be installed on the floating member 110 to measure the temperature of the seawater in the floating member 110 and the salinity sensor 182 may be installed on the floating member 110 The oxygen sensor 183 measures the salinity of the seawater in the floating member 110 and measures the oxygen concentration of the seawater in the floating member 110 installed on the floating member 110, And the salinity can be used to calculate the equilibrium coefficient correction factor between oxygen and liquid.

An apparatus for continuously measuring oxygen and carbon dioxide in a farm is capable of being measured and stored in the water tank 160. The floating member 110 floats in the water tank 160 .

FIG. 2 is a view showing an apparatus for continuously measuring oxygen and carbon dioxide in a farm without biological contamination of a sensor according to another embodiment of the present invention.

Hereinafter, an apparatus for continuously measuring oxygen and carbon dioxide in a farm environment without biological contamination of a sensor according to another embodiment of the present invention will be described with reference to FIG.

As shown in FIG. 2, the apparatus for continuously measuring oxygen and carbon dioxide in a farm environment without biological contamination of the sensor according to another embodiment of the present invention is similar to the embodiment of FIG. 1 in that a floating member 110, A first circulation pipe 140, a second circulation pipe 150, a carbon dioxide partial pressure measuring device 145, and an oxygen partial pressure measuring device 155. The oxygen partial pressure measuring device 155 may include a pipe 120, a dehumidifier 130, a first circulating pipe 140,

The floating member 110 is partially immersed in seawater inhabited by micro-marine organisms, and a head space is formed therein. A bubble introduction pipe 120 is connected to a lower portion of the floating member 110.

A bubble generator 175 may be disposed in the bubble introduction pipe 120. The bubble generator 175 is coupled to the end of the air circulation pipe 170 and the air circulation pipe 170 is connected to the pressure control pipe The air introduced from the pressure control pipe 171 through the air circulation pipe 170 is guided into the bubble introduction pipe 120 through the bubble generator 175 so that the bubble- Can be generated.

Since the bubble introduction pipe 120 is longer than the bubble introduction pipe 120 in the embodiment of FIG. 1, the bubble-shaped air formed in the longer bubble introduction pipe 120 can be separated from the head space of the floating member 110 The pump 125 may be arranged to facilitate the introduction of the fluid into the fluid conduit 111.

The bubble-shaped air thus generated flows into the head space 111 of the floating member 110 and circulates through the dehumidifier 130 to the first circulation pipe 140 and the second circulation pipe 150, The carbon dioxide partial pressure measuring instrument 145 and the oxygen partial pressure measuring instrument 155 provided in the first circulation pipe 140 and the second circulation pipe 150 respectively can measure the carbon dioxide partial pressure and the oxygen partial pressure.

FIGS. 3 and 4 are graphs for explaining a method for continuously measuring oxygen and carbon dioxide in a farm without biological contamination of the sensor.

3 shows the composition ratio of oxygen and carbon dioxide on the air in the head space of the floating member of the present invention and the simultaneous variation of the dissolved oxygen of the surrounding seawater.

As shown in Fig. 3 (a), when the dissolved oxygen in the seawater starts to decrease due to the breathing of the marine life, the gas immediately reaches the equilibrium state between the seawater and the air in contact with the air and the seawater.

As a result, oxygen in the headspace decreases and carbon dioxide increases as shown in Figs. 3 (b) and 3 (c).

Also, as shown in FIG. 4, continuous monitoring values of oxygen and carbon dioxide partial pressure in the equilibrium state between the dissolved oxygen of the seawater in which the organism is inhabited and the gas have a very high explanatory power of more than 99%.

In the foregoing detailed description of the present invention, specific examples have been described. However, various modifications are possible within the scope of the present invention. The technical spirit of the present invention should not be limited to the above-described embodiments of the present invention, but should be determined by the claims and equivalents thereof.

110: Floating member
111: Head space
120: Bubble introduction pipe
125: Pump
130: dehumidifier
140: first circulation pipe
141: Pump
145: Carbon dioxide partial pressure meter
150: second circulation pipe
151: Pump
155: oxygen partial pressure measuring instrument
160: aquarium
161: Seawater
170: air circulation piping
175: Bubble generator
181: Temperature sensor
182: Salinity sensor
183: Oxygen sensor

Claims (6)

A floating member in which a head space is formed by being partially submerged in seawater inhabited by micro-marine organisms;
A bubble introduction pipe that is submerged in the seawater and communicates with a lower portion of the floating member;
A dehumidifier that communicates with one side of the upper portion of the floating member to dehumidify air provided from the floating member;
A first circulation pipe through which air flowing out through the dehumidifier is circulated and flows into the floating member;
A second circulation pipe through which air flowing out through the dehumidifier is circulated and flows into the floating member;
A carbon dioxide partial pressure measuring device for measuring a partial pressure of carbon dioxide in the air provided into the first circulation pipe; And
An oxygen partial pressure measuring device for measuring the oxygen partial pressure in the air provided into the second circulation pipe;
And the oxygen partial pressure of the seawater.
The method according to claim 1,
A first circulation pump installed on the first circulation pipe for introducing the air flowing out of the dehumidifier into the carbon dioxide partial pressure measuring instrument; And
A second circulation pump installed on the second circulation pipe for introducing the air flowing out of the dehumidifier into the oxygen partial pressure measuring device;
Further comprising means for measuring the partial pressure of carbon dioxide and oxygen partial pressure of seawater.
The method according to claim 1,
An air circulation pipe through which air flows through the pressure control pipe; And
A bubble generator coupled to an end of the air circulation pipe and disposed in the bubble introduction pipe;
Further comprising means for measuring the partial pressure of carbon dioxide and oxygen partial pressure of seawater.
The method of claim 3,
A bubble introduction pipe pump disposed above the bubble generator in the bubble introduction pipe for supplying seawater containing bubbles generated in the bubble generator into the floating member;
Further comprising means for measuring the partial pressure of carbon dioxide and oxygen partial pressure of seawater.
The method according to claim 1,
A temperature sensor installed on the floating member and measuring the temperature of seawater in the floating member; And
A salinity sensor installed on the floating member for measuring salinity of seawater in the floating member;
Further comprising means for measuring the partial pressure of carbon dioxide and oxygen partial pressure of seawater.
The method according to claim 1,
A water tank in which the seawater is stored;
Further comprising:
Wherein the floating member continuously measures the partial pressure of carbon dioxide and the partial pressure of oxygen in seawater floating in the water tank.

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