KR20180046091A - Korean hot pepper paste containing red bean and manufacturing method there of - Google Patents
Korean hot pepper paste containing red bean and manufacturing method there of Download PDFInfo
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- KR20180046091A KR20180046091A KR1020160140921A KR20160140921A KR20180046091A KR 20180046091 A KR20180046091 A KR 20180046091A KR 1020160140921 A KR1020160140921 A KR 1020160140921A KR 20160140921 A KR20160140921 A KR 20160140921A KR 20180046091 A KR20180046091 A KR 20180046091A
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- South Korea
- Prior art keywords
- fermentation
- red
- beans
- meju
- days
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Images
Classifications
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/01—Pulses or legumes in form of whole pieces or fragments thereof, without mashing or comminuting
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/05—Mashed or comminuted pulses or legumes; Products made therefrom
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
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- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
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- Chemical & Material Sciences (AREA)
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- Botany (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
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Abstract
Description
본 발명은 팥을 함유한 팥고추장 및 이의 제조방법에 관한 것이다.The present invention relates to a red bean paste containing red bean paste and a process for producing the same.
고추장은 두류 또는 곡류 등을 주원료로 하여 누룩균 등을 배양한 후 고춧가루(6 % 이상) 및 식염 등을 가하여 발효 또는 숙성하거나 숙성 후 고춧가루(6 % 이상) 및 식염 등을 가한 것으로 정의한다(식품과학기술대사전 한국식품과학회 (2008) 식품공전. 광일출판사 5-20-2). Kochujang is defined as the fermentation or aging by adding red pepper powder (6% or more) and salt after incubating koji bruch etc. with main raw materials such as bean curd or cereals or adding red pepper powder (more than 6%) and salt after aging Korean Society of Food Science and Technology (2008) Food Science and Technology.
우리나라 장류에 대한 기원은 확인할 수 없으나 삼국사기에 간장과 된장이 따로 만들어졌다는 기록이 있다는 것으로 보아 통일신라시대 이전부터 제조된 것으로 추정된다. 장류라 함은 동물성 또는 식물성 원료에 누룩균 등을 배양하거나 메주 등을 주원료로 하여 식염 등을 섞어 발효 또는 숙성시킨 것을 제조 또는 가공한 것으로 메주, 한식간장, 양조간장, 산분해간장, 효소분해간장, 혼합간장, 한식된장, 된장, 조미고추장, 춘장, 청국장 및 혼합장 등을 말한다(식품과학기술대사전 한국식품과학회 (2008) 식품공전. 광일출판사 5-20-1). 고추장에 대한 기록은 선조 때 태어나 임진왜란을 겪었던 허균(15691618)의 도문대작에 초시란 용어가 처음 나타나는데, 이것이 바로 오늘날 고추장으로 확인되고 있다. 홍만선이 집필한 산림경제(16431715)에서는 만초장법에 장 담그는 방법이 나오고 증보산림경제(유중림 1766)에는 말린 생선, 곤포 등을 첨가한 최초의 고추장에 대한 기록이 있다. 수문사설(이표 1740) 중 식치방에 순창고추장 조법에는 전복, 큰새우, 홍합, 생강 등을 첨가하여 담근 기록이 있으며, 역주방문(1800년대 중엽)에는 보리쌀을 섞은 고추장의 담금에 대한 기록이 보이고, 조선말기의 우수한 조리서인 빙허각 이씨의 규합총서(1815)에는 고추장 메주를 따로 만들어 고추장을 담그는 방법과 소금으로 간을 맞추는 방법 등을 적어 놓았으며 꿀, 육포, 대추를 섞는 등 소금 대신 청장으로 간을 맞추는 방법에 대하여도 기술하고 있다(Oh HI et al.(2000) Korean J. Food Sci. Technol. 29:1166-1174). The origins of Korean soy sauce can not be confirmed, but it is estimated that soy sauce and soybean paste were made separately in Samguksagi, and it was presumed to have been manufactured before the Unified Silla period. Soy sauce refers to soy sauce, soy sauce, brewed soy sauce, acid-decomposed soy sauce, enzyme-decomposed soy sauce, soy sauce soy sauce, soy sauce soy sauce, (Korean Society of Food Science and Technology (2008) Food Science and Technology Center, Korea Food & Drug Administration, 5-20-1 Kwangil Publishing Co.). The record for kochujang is the first to appear in the book of Hakusun (15691618), which was born during the ancestral time and underwent the Japanese invasion of the Imjin period (15691618), and this is now known as kochujang. In the forest economy (16431715) written by Hong Man Sun, there is a way to immerse the plant in the planting field, and in the case of the enhanced forestry economy (Yuchunlim 1766), there is a record of the first kochujang added with dried fish and packing. There is a record of immersion of the kojukjang in the mouth of the water gate (Table 1740) by adding abalone, big shrimp, mussel, ginger, etc. (1815), an excellent cooking book of the end of the Joseon Dynasty, made a special note on how to dip Kochujang in a separate preparation of Kochujang Meju, and how to mix the salt with the salt. In addition to mixing salt with honey, jerky and jujube, (Oh HI et al. (2000) Korean J. Food Sci. Technol. 29: 1166-1174).
고추장은 우리나라 식생활에서 빼놓을 수 없는 조미료이자 오랫동안 각 가정에서 먹고 있는 전통 발효식품이며, 소화를 촉진시키고, 양질의 단백질 섭취에 도움을 주는 것으로 알려져 있다. 고추장의 주재료인 고추의 매운맛은 위산의 분비를 촉진하고, 입과 위를 자극해 식욕을 증진시켜 주며, 매운맛을 내는 캡사이신은 혈액순환을 촉진시켜 신경통을 치료하는 데 도움을 주고, 체지방 분해와, 지방을 연소시켜 비만 예방과 치료에 효과가 있다. 또한 고추의 비타민 A는 호흡기 감염에 대한 저항력과 면역력을 높이고, 고추에는 비타민 B와 C가 풍부하며, 베타카로틴 성분이 많아 시력 보호에 효과가 있다.Kochujang is an indispensable seasoning food in Korea and it is a traditional fermented food that has been consumed in each household for a long time. It is known to promote digestion and help to obtain high quality protein. Capsicum, the main ingredient of kochujang, promotes the secretion of gastric acid, stimulates the mouth and stomach to promote appetite, and the spicy capsaicin promotes blood circulation to help treat neuralgia, Fat burning is effective in preventing and treating obesity. In addition, vitamin A in the pepper increases resistance and immunity against respiratory infections, pepper is rich in vitamins B and C, and has a high amount of beta carotene, which is effective in protecting eyesight.
고추장은 쌀, 밀가루, 율무 등의 전분질 원료를 수세한 후 가열해서 호화시킨 것에 소맥분 등을 이용하여 제국과정을 거쳐 제조된 코지와 소금 등을 혼합하여 적정조건에서 약 2개월 동안 당화 및 단백질 분해를 일으켜 발효·숙성시킨 것에 고추가루, 물엿, 소르브산, MSG 등을 배합하여 수분함량과 맛을 조정하여 제조하거나, 상기 제조방법에서 코지 대신 메주를 사용하여 건조 고추가루, 물엿, 간장 등으로 조미한 후 약 6개월 동안 숙성시켜 제조한다. 이러한 일반적인 고추장의 제조방법은 약 2 내지 6개월 동안 숙성시키는 단계가 필요하기 때문에 고추장의 제조에 장기간이 소요된다는 단점이 있다. 또한, 고추장은 주로 가정에서 소규모 단위로 제조되어 왔으나, 최근에는 식생활 양식의 변화에 따라 개량된 방식의 공업적 규모로 생산이 가능해졌고, 이러한 경향은 편리성을 추구하는 소비자 욕구와 더불어 점차 확대되고 있으며, 종래의 고추장에 함유되지 않은 새로운 기능성 물질을 첨가하여 영양 성분이 보다 증진된 고추장의 제조가 여전히 요구되고 있다.Kochujang is a mixture of koji and salt, which is prepared by emulsifying wheat flour, etc., after washing the starch raw materials such as rice, flour, and yulmu. And fermenting and aging the mixture to prepare a mixture of chili powder, starch syrup, sorbic acid, MSG and the like to adjust the moisture content and taste. In the above method, Meju is used instead of koji to prepare dried chili powder, syrup, soy sauce And then aged for about 6 months. This general method of producing kochujang requires a step of aging for about 2 to 6 months, which is disadvantageous in that it takes a long time to manufacture kochujang. In addition, Kochujang has been mainly manufactured in small scale at home, but recently it has become possible to produce it on an industrial scale in an improved manner in accordance with the change of the dietary style. This tendency is gradually expanded with the consumer desire for convenience And the addition of a new functional material not contained in the conventional kochujang to further improve the nutritional content is still required.
한편, 팥(赤豆)은 당질, 단백질 등의 에너지류를 함유하는 식품으로 알려져 있으며, 자세하게는 당질 51.13%, 단백질 20.94%, 수분 14.91%, 회분 3.63%, 지방 0.7% 외에도 비타민 B1과 사포닌이 많이 함유되어 있는 고영양가의 콩류로 분류된다. 예로부터 팥은 각기병에 놓은 식품으로 알려져 왔으며, 속이 열한 것과 소갈을 다스리는 식품으로 알려져 있다. 명의별록(名醫別錄)에는 '팥은 한열과 속이 열한 것을 다스리며 소명을 이롭게 하고 소갈에도 좋다'고 되어있고, 약성본초(藥性本草)에는 '팥은 열독을 다스리고 악혈을 없애며 또 비와 위를 튼튼하게 해준다'고 기록되어 있다.Red bean is known as a food containing energy such as carbohydrates and proteins. In detail, it contains 51.13% of carbohydrate, 20.94% of protein, 14.91% of moisture, 3.63% of ash, 0.7% of fat and vitamin B1 and saponin It is classified as high nutritive bean which is contained in large quantity. Since ancient times, red beans have been known to be foods that have been placed on each person's skin, and are known as foods that regulate the digestion. It is said that "red bean is good for the calligraphy, and it is also good for the calligraphy, and it is good for the calligraphy", and the "red bean paste" It is said that 'it strengthens'.
이에, 본 발명자들은 염도가 낮고, 빠른 시간 내에 제조할 수 있는 팥고추장의 제조방법을 설정하고, 이를 통해 팥고추장을 제조하여 고추장 발효중 품질분석을 통해 품질이 우수함을 확인함으로써, 본 발명을 완성하였다.Accordingly, the present inventors set up a process for producing bean kochujang which is low in saltiness and can be produced in a short time, and manufactured bean kochujang through the process, and confirmed that the quality was excellent through quality analysis during the fermentation of kochujang, Respectively.
본 발명의 목적은 찐 쌀과 삶은 콩 및 삶은 팥을 이용하여 팥고추장의 제조 방법 및 이로부터 제조된 팥고추장을 제공하는 것이다.The object of the present invention is to provide a process for producing red bean paste using steamed rice, boiled beans and boiled bean paste, and to provide red bean paste produced from the process.
상기 목적을 달성하기 위하여, 본 발명은In order to achieve the above object,
1) 찐 쌀, 삶은 콩 및 삶은 팥을 포함하는 혼합물을 성형(成型)한 후 발효하여 발효메주를 제조하는 단계;1) molding a mixture containing steamed rice, boiled beans and boiled red beans, and fermenting the mixture to prepare a fermented meju;
2) 상기 단계 1)의 발효메주를 파쇄(破碎)하여 메주가루를 제조하는 단계; 및2) crushing the fermented meju of step 1) to prepare meju powder; And
3) 상기 단계 2)의 메주가루에 당화액, 소금, 고춧가루 및 물을 포함하는 혼합물을 첨가하여 숙성하는 단계를 포함하는 팥고추장의 제조방법을 제공한다.And 3) adding a mixture containing saccharified liquid, salt, red pepper powder and water to the meju powder of step 2), and aging the red bean paste.
아울러, 상기 방법으로 제조된 팥고추장을 제공한다.The present invention also provides a red bean paste produced by the above method.
본 발명의 제조방법으로 제조된 팥고추장은 염도가 낮고, 빠른 시간 내에 제조할 수 있으며, 품질이 우수하므로 기능성 고추장으로 유용하게 사용될 수 있다.The red bean kochujang prepared by the method of the present invention has low salinity, can be produced in a short time, and can be usefully used as a functional kochujang since its quality is excellent.
도 1은 팥고추장의 제조공정을 나타낸 도이다.
도 2는 팥고추장의 발효 기간에 따른 호기성 총 균수 변화를 나타낸 도이다. Y축은 호기성 총 균수를, X축은 발효 기간을 나타낸다.
도 3은 팥고추장의 발효 기간에 따른 유산균 수 변화를 나타낸 도이다. Y축은 유산균 수를, X축은 발효 기간을 나타낸다.
도 4는 팥고추장의 발효 기간에 따른 단백질분해효소(protease) 활성 변화를 나타낸 도이다. Y축은 단백질분해효소(protease) 활성을, X축은 발효 기간을 나타낸다.
도 5는 팥고추장의 발효 기간에 따른 α-아밀라아제(amylase) 활성 변화를 나타낸 도이다. Y축은 α-아밀라아제(amylase) 활성을, X축은 발효 기간을 나타낸다.
도 6은 팥고추장의 발효 기간에 따른 아미노태 질소 함량 변화를 나타낸 도이다. Y축은 아미노태 질소 함량을, X축은 발효 기간을 나타낸다.
도 7은 팥고추장의 발효 기간에 따른 환원당 함량 변화를 나타낸 도이다. Y축은 환원당 함량을, X축은 발효 기간을 나타낸다.1 is a view showing a manufacturing process of red bean paste.
FIG. 2 is a graph showing changes in aerobic total bacterial counts according to the fermentation period of red bean paste koji. The Y axis represents the total aerobic count and the X axis represents the fermentation period.
FIG. 3 is a graph showing the change in the number of lactic acid bacteria according to the fermentation period of red bean paste. FIG. The Y axis represents the number of lactic acid bacteria, and the X axis represents the fermentation period.
FIG. 4 is a graph showing changes in protease activity of fermented soybean kochujang during fermentation. The Y axis represents the protease activity, and the X axis represents the fermentation period.
FIG. 5 is a graph showing changes in the amylase activity of fermented soybean kochujang during fermentation. The Y axis represents the amylase activity and the X axis represents the fermentation period.
6 is a graph showing changes in amino nitrogen content of fermented soybean kochujang during fermentation. The Y axis represents the amino nitrogen content and the X axis represents the fermentation period.
7 is a graph showing the change in reducing sugar content of fermented soybean kochujang during fermentation. The Y axis represents the reducing sugar content, and the X axis represents the fermentation period.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명은 1) 찐 쌀, 삶은 콩 및 삶은 팥을 포함하는 혼합물을 성형(成型)한 후 발효하여 발효메주를 제조하는 단계;The present invention relates to: 1) a step of preparing a fermented meju by molding a mixture containing steamed rice, boiled soybeans and boiled red beans, followed by fermentation;
2) 상기 단계 1)의 발효메주를 파쇄(破碎)하여 메주가루를 제조하는 단계; 및2) crushing the fermented meju of step 1) to prepare meju powder; And
3) 상기 단계 2)의 메주가루에 당화액, 소금, 고춧가루 및 물을 포함하는 혼합물을 첨가하여 숙성하는 단계를 포함하는 팥고추장의 제조방법을 제공한다.And 3) adding a mixture containing saccharified liquid, salt, red pepper powder and water to the meju powder of step 2), and aging the red bean paste.
상기 단계 1)의 찐 쌀은 90℃ 내지 110℃에서 30분 내지 70분 동안 찐 쌀인 것이 바람직하며, 95℃ 내지 105℃에서 50분 내지 70분 동안 찐 쌀인 것이 더욱 바람직하나 이에 한정되지 않는다. 필요 이상으로 온도가 높거나 시간이 길면 불필요한 에너지 낭비의 우려가 있으며, 온도가 지나치게 낮거나 시간이 짧으면 호화가 제대로 이루어지지 않아 메주(발효제)제조에 적절하지 않다. The steamed rice of step 1) is preferably steamed rice at 90 ° C to 110 ° C for 30 minutes to 70 minutes, more preferably steamed rice at 95 ° C to 105 ° C for 50 minutes to 70 minutes, but is not limited thereto. If the temperature is higher than necessary or the time is long, there is a risk of waste of unnecessary energy. If the temperature is too low or the time is short, it is not suitable for making meju (fermentation agent).
상기 단계 1)의 삶은 콩은 90℃ 내지 110℃에서 30분 내지 70분 동안 삶은 콩인 것이 바람직하며, 95℃ 내지 105℃에서 50분 내지 70분 동안 삶은 콩인 것이 더욱 바람직하나 이에 한정되지 않는다. 찌는 온도가 높고 시간이 지나치게 길면, 콩의 조직이 물러질 수가 있고, 찌는 온도가 낮고 시간이 지나치게 짧으면, 콩의 주성분인 단백질 및 전분 등의 구성성분이 발효에 용이하지 않은 상태로 남을 수 있어 바람직하지 않다. Preferably, the boiled beans in step 1) are boiled beans at 90 ° C to 110 ° C for 30 minutes to 70 minutes, more preferably, boiled beans at 95 ° C to 105 ° C for 50 minutes to 70 minutes. If the fermentation temperature is high and the time is too long, the structure of the soybean can be retracted. If the fermentation temperature is low and the time is too short, components such as protein and starch, which are the main components of soybean, I do not.
상기 단계 1)의 삶은 팥은 90℃ 내지 110℃에서 30분 내지 70분 동안 삶은 팥인 것이 바람직하며, 95℃ 내지 105℃에서 50분 내지 70분 동안 삶은 팥인 것이 더욱 바람직하나 이에 한정되지 않는다. 찌는 온도가 높고 시간이 지나치게 길면, 팥의 조직이 물러질 수가 있고, 찌는 온도가 낮고 시간이 지나치게 적으면, 콩의 주성분인 단백질 및 전분 등의 구성성분이 발효에 용이하지 않은 상태로 남을 수 있어 바람직하지 않다. Preferably, the boiled red beans in step 1) are boiled beans at 90 ° C to 110 ° C for 30 minutes to 70 minutes, more preferably bees boiled at 95 ° C to 105 ° C for 50 minutes to 70 minutes. If the temperature of the fermentation is high and the time is too long, the structure of the red beans can be retracted. If the fermentation temperature is low and the time is too low, components such as protein and starch, which are the main components of the soybean, It is not preferable.
상기 단계 1)의 메주 성형 형상은 공 형상의 지름이 4cm 내지 6cm인 메주인 것이 바람직하며, 지름이 4.5cm 내지 5.5cm인 것이 더욱 바람직하나 이에 한정되지 않는다.The meju-forming shape of step 1) is preferably meju having a diameter of 4 cm to 6 cm, more preferably 4.5 cm to 5.5 cm, but is not limited thereto.
상기 단계 1)의 발효 기간은 5일 내지 9일이고, 숙성 온도는 26℃ 내지 30℃이며, 숙성 습도는 60% 내지 80%인 것이 바람직하며, 발효기간이 7일 내지 8일이고, 숙성온도는 27℃ 내지 28℃이며, 숙성 습도는 65% 내지 75%인 것이 더욱 바람직하나 이에 한정되지 않는다. 온도가 지나치게 높으면, 메주가 제조되기 전에 건조가 될수 있고, 온도가 지나치게 낮으면, 발효미생물이 유발되지 않아서 바람직 하지 않다. 발효기간이 길면, 유해미생물의 오염이 우려되고, 발효기간이 짧으면 유용미생물의 적절한 발현이 어려워 적절치 않다. Preferably, the fermentation period of step 1) is 5 to 9 days, the fermentation temperature is 26 to 30 ° C, the fermentation temperature is 60 to 80%, the fermentation period is 7 to 8 days, Is 27 DEG C to 28 DEG C, and the aging humidity is more preferably 65% to 75%, but is not limited thereto. If the temperature is excessively high, the Meju can be dried before the preparation, and if the temperature is too low, the fermentation microorganisms are not induced, which is not preferable. If the fermentation period is long, contamination of harmful microorganisms is a concern, and if the fermentation period is short, it is difficult to appropriately express useful microorganisms.
상기 단계 3)의 당화액은 쌀밥, 엿기름 및 물을 혼합하여 제조하는 것이 바람직하나 이에 한정되지 않는다.The saccharified solution of step 3) is preferably prepared by mixing rice, malt, and water, but is not limited thereto.
상기 전체 당화액 중량에 대해 엿기름은 5 내지 10 중량% 및 쌀밥은 10 내지 30 중량%로 혼합하는 것이 바람직하며, 엿기름은 6 내지 9 중량% 및 쌀밥은 15 내지 25 중량%로 혼합하는 것이 더욱 바람직하나 이에 한정되지 않는다. 엿기름대비 쌀밥의 양이 많으면, 기질 양이 많아 엿기름 유래 당화효소가 부족하게 되어 품질이 적절하지 않게 되며, 엿기름대비 쌀밥의 양이 적으면 필요 이상의 엿기름 유래 효소가 생성되어 바람직하지 않다. It is preferable to mix 5 to 10% by weight of malt with 10 to 30% by weight with respect to the weight of the whole saccharified liquid, more preferably 6 to 9% by weight of malt and 15 to 25% by weight of rice But is not limited thereto. When the amount of cooked rice is large compared to malt, the amount of rice is large, so the saccharification enzyme derived from malt is inadequate and the quality thereof becomes inadequate. When the amount of cooked rice to malt is small, an enzyme derived from malt is not preferred.
상기 단계 3)의 당화액은 6시간 내지 10시간 당화한 것이 바람직하며, 7시간 내지 9시간 당화한 것이 더욱 바람직하나 이에 한정되지 않는다. 당화 시간이 짧으면, 발효과정 중에 관여하는 발효미생물이 이용할 수 있는 탄소원으로 사용되기 위한 작은 분자량의 당들이 적게 되어 좋지 않고, 당화 시간이 길면 필요 이상의 시간 및 에너지 낭비가 되어 적절하지 않다.The saccharified solution of step 3) is preferably saccharified for 6 to 10 hours, more preferably saccharified for 7 to 9 hours, but is not limited thereto. If the saccharification time is short, a small molecular weight sugar for use as a carbon source that can be used by the fermenting microorganisms involved in the fermentation process is small, and if the saccharification time is long, it is not suitable because it requires more time and energy than necessary.
상기 단계 3)의 메주가루 중량에 대해 당화액은 2 내지 5 중량부, 소금은 0.4 내지 0.8 중량부, 고춧가루는 1 내지 3 중량부 및 물은 4 내지 7 중량부로 첨가되는 것이 바람직하며, 상기 단계 3)의 메주가루 중량에 대해 당화액은 3 내지 4 중량부, 소금은 0.5 내지 0.7 중량부, 고춧가루는 1 내지 2 중량부 및 물은 5 내지 6 중량부로 첨가되는 것이 더욱 바람직하나 이에 한정되지 않는다. 과도한 소금을 첨가할 경우 숙성 기간을 비약적으로 증가시킬 수 있고, 또한 고혈압 등 성인병을 유발할 수 있는 문제가 있으며, 소금 함량이 너무 낮으면 된장 부패 위험이 있다. Preferably, 2 to 5 parts by weight of saccharified liquid, 0.4 to 0.8 parts by weight of salt, 1 to 3 parts by weight of red pepper powder and 4 to 7 parts by weight of water are added to the meju powder in step 3) 3 to 4 parts by weight of saccharified liquid, 0.5 to 0.7 parts by weight of salt, 1 to 2 parts by weight of red pepper powder, and 5 to 6 parts by weight of water, based on the weight of meju powder of 3) . When excessive salt is added, the aging period can be dramatically increased, and also, there are problems that can cause adult diseases such as hypertension. When the salt content is too low, there is a risk of misappearance of miso.
상기 단계 3)의 숙성 기간은 20일 내지 50일인 것이 바람직하며, 20일 내지 40일인 것이 더욱 바람직하나 이에 한정되지 않는다. 발효기간이 지나치게 짧으면, 가식 가능한 정도의 발효가 이루어지지 않아 적합하지 않고, 발효기간이 지나치게 길면, 과발효가 일어날 수 있으며, 적절히 발효된 후에는 냉장보관하여 섭취하는 것이 바람직하다.The aging period of the step 3) is preferably 20 days to 50 days, more preferably 20 days to 40 days, but is not limited thereto. If the fermentation period is too short, fermentation is not feasible to the extent that edible fermentation is not possible. If the fermentation period is too long, over fermentation may occur.
아울러, 본 발명은 상기의 방법으로 제조된 팥고추장을 제공한다.In addition, the present invention provides a red bean paste produced by the above method.
본 발명의 구체적인 실시예에서, 본 발명자들은 팥고추장을 제조하고(도 1 참조), 발효기간에 따른 품질특성을 분석하였다. 팥고추장의 호기성 총 균수를 측정한 결과, 발효시작 0일째에는 7.84 log CFU/㎖의 호기성 총 균수를 나타냈으며, 18일까지 비슷한 수준을 유지하다 발효시작 38일 후, 호기성 총 균수는 6.88 log CFU/㎖를 나타냄을 확인하였다(도 2 참조).In a specific embodiment of the present invention, the present inventors prepared red bean paste (see Fig. 1) and analyzed the quality characteristics according to the fermentation period. The total number of aerobic bacteria in red bean paste was 7.84 log CFU / ㎖ at the beginning of fermentation and remained at the same level until 18th day. At 38 days after the start of fermentation, aerobic total bacterial count was 6.88 log CFU / Ml (see Fig. 2).
또한, 본 발명자들은 발효기간에 따른 팥고추장의 유산균 수 변화를 측정한 결과, 발효시작 0일째에는 7.73 log CFU/㎖로 높았으며, 점차 감소하여, 발효시작 38일 후에는 유산균이 검출되지 않음을 확인하였다(도 3 참조).In addition, the present inventors measured the change in the number of lactic acid bacteria in the red bean paste kochujang according to the fermentation period. As a result, it was 7.73 log CFU / ml on the 0th day of fermentation and gradually decreased, and no lactobacilli were detected 38 days after the start of fermentation (See FIG. 3).
또한, 본 발명자들은 발효기간에 따른 팥고추장의 단백질 분해효소(protease) 활성 변화를 측정한 결과, 발효시작 0일째에는 533.00 unit/㎖에서 점차 감소하여, 발효시작 38일 후에는 가장 낮은 활성을 보임을 확인하였다(도 4 참조).In addition, the present inventors measured the protease activity of bean kochujang according to the fermentation period, and it gradually decreased at 533.00 unit / ㎖ at the beginning of fermentation and showed the lowest activity at 38 days after the start of fermentation (See Fig. 4).
또한, 본 발명자들은 발효기간에 따른 팥고추장의 α-아밀라아제(amylase) 활성 변화를 측정한 결과, 발효시작 0일째에는 18.66 unit/㎖로 가장 높았으나 점차 감소하여, 발효시작 38일 후에는 가장 낮은 활성을 보임을 확인하였다(도 5 참조). As a result of measuring the change of amylase activity of bean kochujang according to fermentation period, the highest value was 18.66 unit / ㎖ at the beginning of fermentation, but gradually decreased, and the lowest value was found at 38 days after the start of fermentation (See Fig. 5).
또한, 본 발명자들은 발효기간에 따른 팥고추장의 아미노태 질소 함량 변화를 측정한 결과, 발효시작 0일째에 가장 낮은 13.56 mg%를 나타내었으나 점차 증가하여, 발효시작 28일 후에는 161.32 mg% 까지 증가함을 확인하였다(도 6 참조).The present inventors measured the amino nitrogen content of the red bean kochujang according to fermentation period and found that the lowest value was 13.56 mg% on the 0th day of fermentation but gradually increased to 161.32 mg% after 28 days of fermentation (See FIG. 6).
아울러, 본 발명자들은 발효기간에 따른 팥고추장의 환원당 함량 변화를 측정한 결과, 발효시작 0일째에는 1.00%였으나 점차 증가하여, 발효시작 18일 후에는 1.54%까지 증가하였으나, 발효시작 38일 후에는 초기와 비슷한 1.09%로 감소함을 확인하였다(도 7 참조).In addition, the present inventors measured the reducing sugar content of red bean paste kochujang according to the fermentation period and found that it increased to 1.54% after 18 days from the start of fermentation, though it was 1.00% at the 0th day of fermentation. And decreased to 1.09%, which is similar to the initial value (see FIG. 7).
따라서, 본 발명의 제조방법으로 제조된 팥고추장은 염도가 낮고, 빠른 시간 내에 제조할 수 있으며, 품질이 우수하므로 기능성 고추장으로 유용하게 사용될 수 있다.Thus, the red bean paste produced by the process of the present invention has low salinity, can be produced in a short time, and can be usefully used as a functional kochujang since its quality is excellent.
이하, 본 발명을 하기 실시예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail by the following examples.
단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 의해 한정되는 것은 아니다.However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.
팥고추장의 제조Manufacture of red bean paste
팥고추장을 제조하기 위하여, 쌀, 콩 및 팥을 각각 100℃에서 60분 동안 증자하였다. 상기의 방법으로 제조된 찐쌀가루 4kg과 삶은 콩 8kg 및 삶은 팥 6kg을 혼합하여, 분쇄하고, 지름 5cm 공모양으로 메주를 성형한 후, 메주를 양지에서 1~2일 동안 겉 말림하고, 7-8일 동안 28℃, 상대습도 70%에서 메주를 발효시켰다. 7-8일 후, 발효된 메주를 자연건조 또는 건조기를 사용하여 수분함량이 10%가 되도록 건조시켜 분쇄하고, 상기의 방법으로 분쇄된 4kg의 메주가루에 쌀밥 4kg과 엿기름 1.5kg에 물 15ℓ를 가하여 8시간 당화한 당화액과 2.4kg의 소금, 5kg의 고춧가루 및 21ℓ의 물을 넣고 혼합하여 팥고추장을 제조하였다. 상기의 방법으로 제조된 팥고추장을 항아리에 담아 38일 동안 발효 숙성하였다(도 1). 제조된 날을 0일째로 하여 18일, 38일에 시료를 채취하여 하기 품질분석의 시료로 사용하였다.To make red bean paste, rice, bean and red bean were respectively added at 100 ℃ for 60 minutes. 4 kg of the steamed rice powder prepared by the above method, 8 kg of boiled beans and 6 kg of boiled red beans were mixed and pulverized. The beans were shaped into a ball having a diameter of 5 cm, and the beans were sown for 1-2 days at 7- Meju was fermented at 28 ° C and 70% relative humidity for 8 days. After 7 to 8 days, the fermented meju was dried and pulverized to a moisture content of 10% by using a natural dryer or a dryer. To 4 kg of rice flour and 1.5 kg of malt was added 15 kg of water to 4 kg of meju flour , Saccharified saccharide solution for 8 hours, 2.4 kg of salt, 5 kg of red pepper powder, and 21 liters of water were mixed to prepare red bean paste. The red bean paste prepared in the above manner was put into a jar and fermented for 38 days (Fig. 1). A sample was taken on
팥고추장의 발효 기간에 따른 품질특성 분석Analysis of quality characteristics of fermented soybean kochujang during fermentation
상기 <실시예 1>의 방법으로 제조된 팥고추장의 품질을 분석하기 위하여, 호기성 총 균수와 유산균 수 측정, 단백질 가수분해 효소(protease)활성 측정, α-아밀라아제(α-amylase) 활성 측정, 아미노태 질소 측정 및 환원당 함량 측정을 하기와 같은 방법으로 수행하였다.In order to analyze the quality of red bean paste produced by the method of Example 1, aerobic total bacteria and lactic acid bacteria count, protease activity assay,? -Amylase activity assay, amino Measurement of nitrogen and reducing sugar content were carried out in the following manner.
<2-1> 호기성 총 <2-1> Aerobic gun 균수Number of bacteria 측정 Measure
멸균 생리식염수에 팥고추장 1g을 10진 희석법으로 10단계 희석하고, 희석액을 plate count agar(Difco, Sparks, MD, USA) 배지에 도말하여 30℃에서 24시간 동안 배양한 후, 균수를 계수하여 측정하였다. 실험은 세번 반복하여 수행하였으며, 평균±표준편차(mean±SD)로 값을 나타내었다.1 g of red bean paste was diluted 10-fold with 10-fold dilution method in sterilized physiological saline and the diluted solution was plated on a plate count agar (Difco, Sparks, MD, USA) and cultured at 30 ° C for 24 hours. Respectively. The experiment was repeated three times and the mean ± SD (mean ± SD) value was shown.
그 결과, 발효시작 0일째에는 7.84 log CFU/㎖의 호기성 총 균수를 나타냈으며, 18일까지 비슷한 수준을 유지하다 발효시작 38일 후, 호기성 총 균수는 6.88 log CFU/㎖를 나타냄을 확인하였다(도 2).As a result, the aerobic total number of bacteria was 7.84 log CFU / ml on the 0th day of fermentation and remained at the same level until 18th day. After 38 days from the start of fermentation, the aerobic total number of bacteria was found to be 6.88 log CFU / ml 2).
<2-2> <2-2> 유산균수Number of lactic acid bacteria 측정 Measure
멸균 생리식염수에 팥고추장 1g을 10진 희석법으로 10단계 희석하고, 희석액을 MRS agar(Difco, Sparks, MD, USA) 배지에 도말하여 37℃에서 48시간 동안 배양한 후, 균수를 계수하여 측정하였다. 실험은 세번 반복하여 수행하였으며, 평균±표준편차(mean±SD)로 값을 나타내었다.1 g of red bean paste was diluted 10-fold with 10-fold dilution method in sterilized physiological saline, and the diluted solution was plated on a MRS agar (Difco, Sparks, MD, USA) medium and cultured at 37 ° C for 48 hours. . The experiment was repeated three times and the mean ± SD (mean ± SD) value was shown.
그 결과, 발효시작 0일째에는 7.73 log CFU/㎖로 높았으며, 점차 감소하여 발효시작 18일 후에는 6.12 log CFU/㎖를, 발효시작 38일 후에는 유산균이 검출되지 않음을 확인하였다(도 3).As a result, it was confirmed that lactic acid bacterium was not detected after 38 days after the start of fermentation and 6.12 log CFU / ml after 18 days from the start of fermentation, which was found to be 7.73 log CFU / ).
<2-3> 단백질분해효소(protease) 활성 측정<2-3> Measurement of protease activity
단백질분해효소(protease)의 활성도는 Anson의 방법을 변형하여 측정하였다(Kim HJ et al.1998. Korean J Food Sci Technol. 30:1333-1338.). 시료 추출액 1㎖에 0.6% 카세인(casein) 기질용액(0.2M phosphate buffer, pH 7.0)을 넣고 37℃에서 10분간 반응시켰다. 반응 후, 0.44M 트라이클로로아세트산(trichloroacetic acid; TCA) 5㎖를 넣어 반응을 중지시켰다. 실온에서 30분간 방치한 다음 상기 시료 추출액을 여과지(No.2, Whatman)로 여과시키고, 여과한 여액 2㎖에 0.55M Na2CO3용액 5㎖와 3배 희석된 폴린시약(Folin reagent) 용액 1㎖를 넣어 실온에서 30분간 반응시킨 후, 660nm에서 흡광도를 측정하였다. 이 반응조건하에서 1분 동안 타이로신(tyrosine) 1㎍을 유리시키는 효소량을 1unit으로 환원하여 나타내었다. 실험은 세번 반복하여 수행하였으며, 평균±표준편차(mean±SD)로 값을 나타내었다.The activity of protease was measured by modifying Anson's method (Kim HJ et al. 1998. Korean J Food Sci Technol. 30: 1333-1338). A 0.6% casein substrate solution (0.2 M phosphate buffer, pH 7.0) was added to 1 ml of the sample extract and incubated at 37 ° C for 10 minutes. After the reaction, 5 ml of 0.44 M trichloroacetic acid (TCA) was added to terminate the reaction. After allowing to stand at room temperature for 30 minutes, the sample extract was filtered with a filter paper (No. 2, Whatman), and 5 ml of a 0.55
그 결과, 발효시작 0일째에는 533.00 unit/㎖에서 점차 감소하여, 발효시작 18일 후에는 366.33 unit/㎖를, 발효시작 38일 후에는 가장 낮은 223.17unit/㎖의 활성을 보임을 확인하였다(도 4).As a result, it was confirmed that the activity decreased gradually from 533.00 unit / ml on the 0th day of fermentation to 366.33 unit / ml after 18 days of fermentation and 223.17unit / ml of activity after 38 days of fermentation 4).
<2-4> α-아밀라아제(amylase) 활성 측정≪ 2-4 > Measurement of? -Amylase activity
α-아밀라아제(amylase)활성 측정은 1% 가용성 전분(0.02M phosphate buffer, pH 7.0) 3㎖에 시료 추출액 1㎖를 첨가하여 40℃에서 10분간 반응시킨 후, 1M HCl 10㎖를 넣어 반응을 중지시켰다. 반응을 중지시킨 후, 요오드화 용액(0.005% I2+0.05% KI) 10㎖를 넣고 발색시킨 후, 660nm에서 흡광도를 측정하여, 효소액 1㎖가 나타내는 흡광도를 효소 역가로 표시하였다. 실험은 세번 반복하여 수행하였으며, 평균±표준편차(mean±SD)로 값을 나타내었다.Amylase activity was measured by adding 1 ml of sample extract to 3 ml of 1% soluble starch (0.02 M phosphate buffer, pH 7.0), reacting at 40 ° C for 10 minutes, adding 10 ml of 1M HCl to stop the reaction . After discontinuing the reaction, 10 ml of iodination solution (0.005% I 2 + 0.05% KI) was added and developed. The absorbance at 660 nm was measured and the absorbance of 1 ml of the enzyme solution was indicated in the enzyme reverse phase. The experiment was repeated three times and the mean ± SD (mean ± SD) value was shown.
그 결과, 발효시작 0일째에는 18.66 unit/㎖로 가장 높았으나 점차 감소하여, 발효시작 18일 후에는 7.52 unit/㎖을 보였고, 발효시작 38일 후에는 1.50 unit/㎖ 까지 감소하여 가장 낮은 활성을 보임을 확인하였다(도 5). As a result, the highest activity was 18.66 unit / ㎖ at the beginning of fermentation, but gradually decreased to 7.52 unit / ㎖ at 18 days after fermentation and decreased to 1.50 unit / ㎖ at 38 days after fermentation. (Fig. 5).
<2-5> <2-5> 아미노태Amino acid 질소 함량 측정 Nitrogen content measurement
아미노태 질소 함량은 단백질분해효소(protease)의 작용에 의하여 단백질이 아미노산의 형태로 분해되는 정도를 나타낸 것으로, 발효도 평가 및 장류 발효식품의 품질과 구수한 맛의 지표로 사용되고 있다. 아미노태 질소 함량은 Formol 적정법으로 측정하였다. 시료추출액 5㎖, 중성 포르말린(formalin)용액, 증류수 10㎖를 넣은 플라스크에 0.5% 페놀프탈레인(phenolphthalein) 용액을 2~3방울 가한 후, 0.1N NaOH로 미홍색이 될 때까지의 적정량과 시료 5㎖, 증류수 20㎖를 넣은 플라스크에 0.5% 페놀프탈레인(phenolphthalein) 용액을 2~3방울 가한 후, 0.1N NaOH로 미홍색이 될 때까지의 적정량을 이용하여 아미노태 질소 함량을 산출하였다. 실험은 세번 반복하여 수행하였으며, 평균±표준편차(mean±SD)로 값을 나타내었다.Amino nitrogen content is the degree of degradation of protein in the form of amino acid by the action of protease, and it is used as an index of fermentation evaluation, quality of fermented food, and taste. Amino nitrogen content was measured by the Formol titration method. Add 2 ~ 3 drops of 0.5% phenolphthalein solution to a flask containing 5 ml of sample extract, formalin solution and 10 ml of distilled water. Then, add 0.1 ml of 0.1 N NaOH, , And 20 ml of distilled water was added 2 ~ 3 drops of 0.5% phenolphthalein solution, and then the amount of amino nitrogen was calculated using 0.1N NaOH in an appropriate amount from the time until it became deep red. The experiment was repeated three times and the mean ± SD (mean ± SD) value was shown.
그 결과, 발효시작 0일째에 가장 낮은 13.56 mg%를 나타내었으나 점차 증가하여, 발효시작 18일 후에는 86.51 mg%에서 발효시작 28일 후에는 161.32 mg% 까지 증가하였다. 상기 <실시예 2-4>의 단백질분해효소(protease) 활성과의 관계를 보면, 단백질분해효소(protease)의 경우 발효기간 중 감소하는 반면 아미노태의 경우 발효가 될수록 증가하여 반비례함을 확인하였다(도 6).As a result, the lowest value of 13.56 mg% was shown on the 0th day of fermentation but it gradually increased to 86.51 mg% after 18 days of fermentation and 161.32 mg% after 28 days of fermentation. The relationship between protease activity of the above <Example 2-4> and the protease activity of the protease was decreased during the fermentation period, while it was inversely proportional to the fermentation of the protease activity 6).
<2-6> 환원당 함량 측정<2-6> Measurement of reducing sugar content
환원당 함량은 디니트로살리실산 법(Dinitrosalicylic acid method, DNS 법)(Miller, G. L. 1960. Anal. Biochem. 2, 127-132.)으로 측정하였다. 시험관에 DNS 시약 3㎖와 희석한 시료용액 1㎖를 가하고 진탕한 후, 끓는 수조에서 5분간 방치하고, 즉시 얼음 수조에서 냉각시킨 다음 550nm에서 흡광도를 측정하고, 글루코스(glucose)를 이용한 표준곡선으로부터 환원당 함량을 산출하였다. 실험은 세번 반복하여 수행하였으며, 평균±표준편차(mean±SD)로 값을 나타내었다.The reducing sugar content was measured by the dinitrosalicylic acid method (DNS method) (Miller, G. L. 1960. Anal. Biochem. 2, 127-132). 3 ml of the DNS reagent and 1 ml of the diluted sample solution were added to the test tube, shaken, left in a boiling water bath for 5 minutes, immediately cooled in an ice water bath, and then absorbance was measured at 550 nm. From the standard curve using glucose The reducing sugar content was calculated. The experiment was repeated three times and the mean ± SD (mean ± SD) value was shown.
그 결과, 발효시작 0일째에는 1.00%였으나 점차 증가하여, 발효시작 18일 후에는 1.54%까지 증가하였으나, 발효시작 38일 후에는 초기와 비슷한 1.09%로 감소함을 확인하였다(도 7).As a result, it was 1.00% at the 0th day of fermentation but gradually increased to 1.54% at the beginning of fermentation, but decreased to 1.09% at the 38th day after the start of fermentation (Fig. 7).
Claims (12)
2) 상기 단계 1)의 발효메주를 파쇄(破碎)하여 메주가루를 제조하는 단계; 및
3) 상기 단계 2)의 메주가루에 당화액, 소금, 고춧가루 및 물을 포함하는 혼합물을 첨가하여 숙성하는 단계를 포함하는 팥고추장의 제조방법.
1) molding a mixture containing steamed rice, boiled beans and boiled red beans, and fermenting the mixture to prepare a fermented meju;
2) crushing the fermented meju of step 1) to prepare meju powder; And
3) adding a mixture containing saccharified liquid, salt, red pepper powder, and water to the meju powder of step 2), and aging the red bean paste.
The method of claim 1, wherein the steamed rice of step 1) is steamed rice at 90 ° C to 110 ° C for 30 minutes to 70 minutes.
The method of claim 1, wherein the boiled beans in step 1) are boiled beans at 90 ° C to 110 ° C for 30 minutes to 70 minutes.
The method of claim 1, wherein the boiled red beans in step 1) are boiled beans at 90 to 110 ° C for 30 to 70 minutes.
[2] The method according to claim 1, wherein the meju-molding in step 1) is a meju having a diameter of 4 cm to 6 cm.
The method of claim 1, wherein the fermentation period of step 1) is 5 days to 9 days, the fermentation temperature is 26 to 30 ° C, and the fermentation temperature is 60 to 80%.
The method according to claim 1, wherein the saccharified solution of step 3) is prepared by mixing rice, malt, and water.
[7] The method of claim 7, wherein the malt is 5 to 10 wt% and the rice is 10 to 30 wt% based on the total weight of the saccharified liquid.
[7] The method of claim 7, wherein the saccharified liquid is saccharified for 6 to 10 hours.
[3] The method according to claim 1, wherein 2 to 5 parts by weight of saccharified liquid, 0.4 to 0.8 parts by weight of salt, 1 to 3 parts by weight of red pepper powder and 4 to 7 parts by weight of water are added to the meju powder in step 3) ≪ RTI ID = 0.0 > 1.
The method of claim 1, wherein the fermentation period of the step 3) is 20 days to 50 days.
A red bean paste produced by the method of any one of claims 1 to 11.
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KR20200114277A (en) * | 2019-03-28 | 2020-10-07 | 이미건 | Manufacturing method of apricot korean Kochujang using fermented rice-soybean lump |
KR20200129886A (en) * | 2019-05-10 | 2020-11-18 | 엔텍바이오 주식회사 | Composition of traditional red pepper paste using natural fermented species and it's manufacturing method |
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KR20200114277A (en) * | 2019-03-28 | 2020-10-07 | 이미건 | Manufacturing method of apricot korean Kochujang using fermented rice-soybean lump |
KR20200129886A (en) * | 2019-05-10 | 2020-11-18 | 엔텍바이오 주식회사 | Composition of traditional red pepper paste using natural fermented species and it's manufacturing method |
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