KR20180043699A - Mischell emulsionized tocopherol composition and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a composition for emulsifying tocopherol by using micellar emulsion technology and, more specifically, to a micellar emulsion tocopherol composition and a manufacturing method thereof to prevent oxidation by enclosing natural tocopherol in micelles, to increase an absorption speed by increasing storage stability and dispersibility, and to be easily added to food, cosmetics, feed, and the like. The micellar emulsion tocopherol composition manufactured by the manufacturing method of the present invention enables long term storage and easy handling by ensuring oxidation stability during storage which is a problem of an antioxidant by enclosing natural tocopherol in micro-micelles. The present invention has increased dispersibility such that there is no need to have food additives added to food for antioxidant purposes, is easily mixed with other components, and is well dispersed than in the body during digestion to increase an absorption speed, thereby being very useful in food, cosmetics, and feed industries. The micellar emulsion tocopherol composition includes: (a) 1-90 parts by weight of natural tocopherol; (b) 10-99 parts by weight of fat and oil; (c) 1-20 parts by weight of glycerin fatty acid ester; and (d) 1-30 parts by weight of polyglycerin fatty acid ester.

Description

TECHNICAL FIELD The present invention relates to a micelle-tocopherol composition and a method for producing the same,

The present invention relates to a composition for emulsifying tocopherol using micellar emulsification technology and a method for preparing the same, more particularly, to a composition for preventing the oxidation of natural tocopherol in micelles, increasing storage stability and dispersibility, And to be easily added to foods, cosmetics, feeds and the like, and to a process for producing the tocopherol composition.

In general, tocopherol is called vitamin E and is a fat-soluble ingredient distributed along with the fat. As a natural antioxidant in foods, it is widely known to prevent the oxidation of food. In living organisms, tocopherol has physiological functions and nutritional activities such as prevention of circulatory diseases and cancer, prevention of aging and degenerative diseases, and anti- It has been reported that periodic administration of alpha -tocopherol inhibits progression of arteriosclerosis and prevents cataracts. The blood cholesterol content in vivo has been reported to decrease the level of tocopherol, and not only promote wound healing, but also has an excellent effect on scar treatment of burns. In addition, it protects the respiratory mucous membrane from air pollution and has various physiological effects such as detoxifying action, preservation function of intracellular membrane, skin eczema, edema, improvement of blood lesion, and improvement of immune function. However, since it is impossible to directly synthesize tocopherol in vivo, it must be ingested from food or food. In order to maximize the effect of tocopherol, the water soluble component of tocopherol is added to the body, the water content of the body is about 70% It is an urgent necessity. To date, many studies on tocopherol have been carried out by many researchers, and many studies have been conducted on tocopherol, which is a soluble and soluble compound, in not only the action mechanism in vivo but also the antioxidant properties in food. However, studies on the water solubility of tocopherol have not been found in the field of food.

Conventional techniques for improving the body stability of fat-soluble ingredients include preparing macroscopic capsules or macroscopic capsules that are visible to the naked eye using starch, alginic acid, melamine, ethylcellulose, chitosan, It is also possible to prescribe a higher concentration than necessary in the prescription and to take into consideration the loss in the distribution period, or to add a stabilizer, antioxidant, and additives such as hydrulonic acid and vitamin C at a high concentration, A method of blending a large amount of a coating agent was used. However, in the conventional method, the stability of the raw material itself can be said to be constant with the functional material, the added stabilizer, the coating agent, the antioxidant and the like. However, once added to the formulations of foods, medicines, There is a problem that the physical properties such as viscosity, pH and the like change with time, which causes problems in the product itself.

On the other hand, liquid crystals behave like liquids in terms of viscosity and fluidity due to their relatively lower molecular mobility than liquid phases due to regular alignment between molecules, but they have unique properties such as crystalline properties in light scattering and reflection properties Lt; / RTI > Due to such relatively small intermolecular mobility, liquid crystals exhibit the property of improving the stability of unstable materials in the field of cosmetics, medicines and the like.

The most important feature of such liquid crystal emulsification is that it has a lamellar structure film, which is superior in moisture retention to general emulsification. In addition, when the liquid crystal emulsification is carried out, there is an advantage that both the stabilization of the physiologically active substance which is significantly higher than that of the conventional oil phase / water phase and water / oil phase emulsion and simultaneously the sustained release of the physiologically active substance are satisfied. However, there was no composition in which natural tocopherol, which is a substance unstable to oxidation, was enclosed in micro-micelles in a stable form.

Accordingly, the inventors of the present invention have developed a micelle-emulsifying tocopherol composition having improved solubility and improved absorption so that many physiologically active functions of fat-soluble tocopherol can be expressed even in an aqueous solution state.

KR 10-2016-0008223 A KR 10-2015-0102058 A KR 10-2015-0058500 A KR 10-2003-0071009 A

In order to solve the problems of the prior art as described above, a problem to be solved by the present invention is to stabilize unstable natural tocopherol by a large change in oxidation and polarity, to improve functionality, to increase dispersibility upon addition to food, And to provide a method for producing the micelle-tocopherol composition.

In order to solve the above problems, the present invention provides a composition comprising (a) 1 to 90 parts by weight of natural tocopherol; (b) 10 to 99 parts by weight of a fat-containing fatty acid chain; (c) 1 to 20 parts by weight of a glycerin fatty acid ester; And (d) 1 to 30 parts by weight of a polyglycerol fatty acid ester.

Preferably, the natural tocopherols are selected from the group consisting of alpha-, beta-, gamma- and delta-tocopherol.

Preferably, the heavy chain fatty acid comprises at least 90% by weight of caprylic acid, capric acid and mixtures thereof.

The fatty acid constituting the glycerin fatty acid ester and the polyglycerin fatty acid ester is preferably selected from the group consisting of oleic acid, linoleic acid, linolenic acid, and mixtures thereof.

The polyglycerin constituting the polyglycerin fatty acid ester is preferably selected from the group consisting of diglycerin, triglycerin, tetraglycerin, pentaglycerin, hexaglycerin, decaglycerin and mixtures thereof.

The diameter of the micelle is preferably 0.1 to 500 탆.

The composition is preferably used in food, cosmetics or feed.

(B) 10 to 90 parts by weight of a fat-containing fatty acid-containing fat; (c) 1 to 20 parts by weight of a glycerin fatty acid ester at 35 to 40 占 폚; Mixing and homomixing at 2,000 to 3,000 rpm for 5 to 10 minutes while introducing nitrogen; (2) passing the homomixed mixture through an ultrahigh pressure homogenizer in step (1); And (3) adding 1 to 30 parts by weight of polyglycerol fatty acid ester to the mixture passed through the ultra-high pressure homogenizer in the step (2), mixing the mixture for 3 to 5 minutes and then cooling the mixture to 30 ° C or less The present invention provides a method for producing a micellar emulsified tocopherol composition.

The micellar emulsifying tocopherol composition prepared by the method of the present invention can be stored for a long period of time and is easy to handle since the natural tocopherol is enclosed in the micro-micelles and oxidation stability during storage, which is a problem of the antioxidant, is secured. In addition, since the dispersibility is increased, the food additive added to the food for the purpose of antioxidation is not needed, and it can be easily mixed with the other ingredients and can be more easily dispersed in the body at the time of ingestion, And is very useful in the feed industry.

FIG. 1 shows emulsion stability test results of a composition prepared from an embodiment of the present invention using a turboscan.
2 shows the emulsion stability test results of the composition prepared from the comparative example of the present invention using a turbine scan.

Hereinafter, the present invention will be described in detail.

The inventors of the present invention have conducted intensive studies in order to secure the oxidation stability in natural tocopherol and to increase the dispersibility and the absorption rate when added to foods. As a result, it has been found that the oil containing natural tocopherol is enclosed in micelles, It has been confirmed that the stability and dispersibility of natural tocopherol are improved due to the relatively low intermolecular mobility, and the present invention has been accomplished on the basis thereof.

Accordingly, the present invention provides a composition comprising: (a) 1 to 90 parts by weight of natural tocopherol; (b) 10 to 99 parts by weight of a fat-containing fatty acid chain; (c) 1 to 20 parts by weight of a glycerin fatty acid ester; And (d) 1 to 30 parts by weight of a polyglycerol fatty acid ester.

Tocopherol is a group of derivatives of fat-soluble vitamin E that has anti-infertility effect, and is a viscous-yellow viscous liquid, also called vitamin E. α-, β-, γ-, and δ-tocopherols exist naturally, and the most common and the most bioactive is α-tocopherol, which is used as a synonym for vitamin E. Vitamin E has been found as an antiserum vitamin in animals. An important function in living organisms is the oxidation of unsaturated fatty acids, which constitute cell membranes, as anti-oxidants, Inhibits cell membrane damage and prevents tissue damage. The daily requirement is 10 ~ 15mg for adults. It is especially abundant in the freezing of wheat and rice, but rarely in animal fats. Deficiency is red blood cell hemolysis, anemia, and infertility. The antioxidant effect of food is the highest in α-, followed by β- (33% of α-), γ-, and δ-, and generally used to prevent oxidation of persistent foods, but the antioxidant power is not so strong. The effect varies depending on the conditions of use. The natural tocopherols are preferably selected from the group consisting of alpha-tocopherol, beta-tocopherol, gamma-tocopherol and delta-tocopherol. The content of natural tocopherol is preferably 1 to 90 parts by weight. If the content is less than 1 part by weight, the effect of addition is insignificant. If the content is more than 90 parts by weight, synergistic effect is not obtained due to excess input.

The heavy chain fatty acid may be caprylic acid, capric acid, or a mixture thereof, and the content thereof is preferably 90 wt% or more.

The glycerin fatty acid ester is preferably selected from the group consisting of oleic acid, linoleic acid, linoleic acid, and mixtures thereof. It is most preferable that the glycerin fatty acid ester contains oleic acid in an amount of 80% or more in terms of oxidation stability and digestion and absorption Do. At this time, the glycerin fatty acid ester containing oleic acid serves as a base for forming the primary micelle. The content of the glycerin fatty acid ester is preferably 1 to 20 parts by weight. If the content of the glycerin fatty acid ester is less than 1 part by weight, the effect of forming the micelle upon addition is insignificant. If it exceeds 20 parts by weight, I can not.

The polyglycerin fatty acid ester acts as a secondary micelle forming agent together with a glycerin fatty acid ester which is a primary micelle forming agent and forms strong micelles to block natural tocopherol from the outside to maximize stability and to disperse hydrophilicity extremely It improves usability by making it good. Wherein the constituent fatty acid is a polyglycerin fatty acid prepared from a fatty acid selected from the group consisting of oleic acid, linoleic acid, linolenic acid, and mixtures thereof, and the polyglycerin is selected from the group consisting of diglycerin, triglycerin, tetraglycerin, pentaglycerin, hexaglycerin, decaglycerin, And mixtures thereof. When the content of the polyglycerol fatty acid ester is less than 1 part by weight, it is difficult to form micelles and the water dispersibility is poor. When the content of the polyglycerol fatty acid ester is more than 30 parts by weight, I can not.

The natural tocopherol in the composition of the present invention is contained inside the emulsifier film stably formed by the micelle forming agent. Because these micelles prevent direct contact with external stimuli, they protect tocopherol, which is weak to oxidation, and increase water dispersibility, which facilitates mixing when added to other foods and improves water absorption in the body. In addition, stability is improved due to the relatively small intermolecular mobility of the micelle.

The diameter of the tocopherol composition sealed in the micelle is preferably in the range of 0.1 to 500 mu m. When the diameter is less than 0.1 탆, the liquid crystal forming time is long, while the size is relatively small. However, when the diameter is more than 500 탆, the possibility of lipid shedding increases and the digestion absorption time becomes longer not.

(A) 10 to 90 parts by weight of natural tocopherol; (b) 10 to 90 parts by weight of a fat-containing fatty acid; (c) glycerin fatty acid ester 1 to 20 Mixing the parts by weight at 35 to 40 占 폚 and homomixing at 2,000 to 3,000 rpm for 5 to 10 minutes while introducing nitrogen; (2) passing the homomixed mixture through an ultrahigh pressure homogenizer in step (1); And (3) adding 1 to 30 parts by weight of polyglycerol fatty acid ester to the mixture passed through the ultra-high pressure homogenizer in the step (2), mixing the mixture for 3 to 5 minutes and then cooling the mixture to 30 ° C or less .

The above manufacturing method will be described in detail for each step.

First, 10 to 99 parts by weight of a fat-containing fatty acid selected from the group consisting of (a) 1 to 90 parts by weight of natural tocopherol, (b) caprylic acid, capric acid and mixtures thereof, (c) glycerin fatty acid ester 1 to 20 parts by weight are mixed at 35 to 40 占 폚 and homomixed at 2,000 to 3,000 rpm for 5 to 10 minutes while introducing nitrogen. If the heating temperature is lower than 35 ° C, the mixing is not uniform, and the formation of micelles between tocopherol and glycerin fatty acid esters becomes difficult due to the influence of the viscosity. When the temperature exceeds 40 ° C, oxidation of natural tocopherol occurs Which is undesirable. At this time, it is preferable that the homomixing rotational speed is 2,000 to 3,000 rpm, and when the rotational speed is 2,000 rpm or less, the shape of the micelles may be uneven. If the rotational speed is 3,000 rpm or more, The raw material may not be sufficiently enclosed in the micelle.

The homomixed mixture is then passed through a high pressure homogenizer in this step. The homomixed mixture passes through a high pressure homogenizer and the size of the particles is reduced to a fine size and the lipophilic portion of the amphipathic glycerin fatty acid ester tends to form micelles at the interface of natural tocopherol. In a preferred embodiment, the pressure of the high pressure homogenizer may be between 10,000 and 18,000 psi.

Finally, 1 to 30 parts by weight of a polyglycerin fatty acid ester as a micelle forming agent is added and mixed, and after homomixing for 3 to 5 minutes, the mixture is cooled to 30 캜 or lower. At this time, the lipophilic group of the polyglycerin fatty acid ester and the lipophilic group of the glycerin fatty acid ester associate with each other to form stable micelles. The fatty acid of the polyglycerin fatty acid ester is preferably selected from the group consisting of oleic acid, linoleic acid, linolenic acid, and mixtures thereof. Also, the polyglycerin may be diglycerol, triglycerol, tetraglycerol, pentaglycerol, hexaglycerol, decaglycerol and mixtures thereof.

The micellar emulsifying tocopherol composition thus prepared can be stored for a long period of time by ensuring its oxidation stability and its dispersibility is increased. When it is added to foods, it can be easily mixed with other components and dispersed more effectively in the body when consumed. It is expected to be.

The micellar emulsified tocopherol composition of the present invention can be used in foods, cosmetics or feed.

Hereinafter, the present invention will be described in more detail with reference to specific examples. The following examples are illustrative of preferred embodiments of the present invention, and it is apparent that the scope of the present invention is not limited by the description of the following examples.

[Example]

Example

2,250 g (trade name: d-alpha-Tocopherol DSM) of di-alpha tocopherol containing 96% of mixed tocopherol, 2,250 g (trade name: Radia 7104) of a fat containing medium chain fatty acid, 150 g of glycerin fatty acid ester containing 82% (Trade name: Excel O-95R KAO Corporation) was mixed at 35 DEG C and then homomixed at 3,000 rpm for 10 minutes while nitrogen was supplied. After the homomixed mixture was passed twice through a high-pressure homogenizer, 350 g of polyglycerol fatty acid ester (trade name: DS-100A Riken Vitamin) was added and homomixed for 5 minutes and then cooled to 25 캜 to prepare a micelle emulsion composition containing tocopherol Respectively.

Comparative Example

Di-containing mixed tocopherols 96% alpha tocopherol and lecithin 2,250g 100g (trade name: SOLEC ^® Soy Lecithin DANISCO) preparing a mixture at 40 ℃, and 2,400g sorbitol (trade name: D- sorbitol solution ㈜ LG Household & Health Care) and 250 g of polyglycerin fatty acid ester (trade name: PO-100 Riken Vitamin) was mixed at 40 캜 and emulsified. Mixed tocopherol was gradually added to sorbitol while nitrogen was added, homomixed for 20 minutes, and then passed through a high pressure homogenizer to prepare an emulsion.

Experimental Example

Oxidation stability measurement

Oxidative stability was measured by induction time using Metrom 743 Rancimat at 130 ° C and 20 L / min aeration.

sample Oxidation induction period (hr) Example 9.2 hr Comparative Example 6.8hr

As shown in Table 1, the compositions of the examples of the present invention were confirmed to have high oxidation stability because the oxidation induction period was longer than that of the comparative examples.

Measurement of Antioxidant Efficacy

The antioxidative activity of the sample was measured indirectly by measuring the degree of reduction of radicals using a stable free radical, DPPH, using a spectrophotometer.

sample 1 mg / ml 5mg / ml Natural tocopherol 54% 68% Example
(Containing 45% natural tocopherol) 50% 66% Comparative Example
(Containing 45% natural tocopherol) 40% 52%

As shown in Table 2, it was confirmed that the composition of the present invention did not decrease the antioxidative activity of natural tocopherol, and the antioxidative activity of the comparative example was greatly reduced.

Emulsion stability experiment using the Turb scan

The emulsion stability of the emulsions prepared in Examples and Comparative Examples was measured using Turbiscan, which is used to measure the stability of emulsions.

Experimental conditions: temperature 55 ° C, duration 6 days

As shown in FIGS. 1 and 2, the micelle structure compositions of the examples prepared according to the method of the present invention are excellent in creaming, sedimentation and flocculation (coalescence) Stability.

Claims (8)

(a) 1 to 90 parts by weight of natural tocopherol;
(b) 10 to 99 parts by weight of a fat-containing fatty acid chain;
(c) 1 to 20 parts by weight of a glycerin fatty acid ester; And
(d) 1 to 30 parts by weight of a polyglycerol fatty acid ester. The method according to claim 1,
Wherein said natural tocopherol is selected from the group consisting of alpha-, beta-, gamma- and delta-tocopherol. The method according to claim 1,
Wherein the heavy chain fatty acid comprises at least 90% by weight of caprylic acid, capric acid and mixtures thereof. The method according to claim 1,
Wherein the fatty acid constituting the glycerin fatty acid ester and the polyglycerin fatty acid ester is selected from the group consisting of oleic acid, linoleic acid, linolenic acid, and mixtures thereof. The method according to claim 1,
Wherein the polyglycerin constituting the polyglycerin fatty acid ester is selected from the group consisting of diglycerin, triglycerin, tetraglycerin, pentaglycerin, hexaglycerin, decaglycerin, and mixtures thereof. 6. The method according to any one of claims 1 to 5,
Wherein the micelle has a diameter of 0.1 to 500 탆. (1) 10 to 90 parts by weight of (a) 10 to 90 parts by weight of natural tocopherol, (b) 10 to 90 parts by weight of a fat-containing fatty acid chain, (c) 1 to 20 parts by weight of a glycerin fatty acid ester, Homomixing at 2,000 to 3,000 rpm for 5 to 10 minutes while feeding;
(2) passing the homomixed mixture through an ultrahigh pressure homogenizer in step (1); And
(3) adding 1 to 30 parts by weight of a polyglycerol fatty acid ester to the mixture passed through the ultra-high pressure homogenizer in the step (2), mixing and homogenizing for 3 to 5 minutes and then cooling to 30 DEG C or less A process for preparing a micelle-emulsifying tocopherol composition. The method according to claim 6,
Wherein the composition is used in foods, cosmetics or feeds.

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