KR20180031438A - A natural nitrite containing spinach extract and color developing method of meat having the same - Google Patents

Abstract

The present invention relates to natural nitrite using a spinach extract, and a method for coloring raw meat by containing the same. To this end, the method comprises the following steps: producing the spinach extract by using powder obtained by grinding freeze-dried spinach; and adding sugar and Lactobacillus farciminis (KCTC 3618) to the spinach extract, and fermenting the same for 15-95 hours at 20-35°C. The nitrite can be used as a substitute for synthetic nitrite and used in the manufacture of meat products instead of synthetic nitrites.

Description

TECHNICAL FIELD The present invention relates to a natural nitrite-containing spinach extract and a color developing method of producing a natural nitrite salt,

The present invention relates to a natural nitrite obtained by converting a nitrogen source of a plant into a nitrite ion using a biotransformation technique that can replace synthetic nitrite used in meat products, and a method for coloring raw meat including the same.

Generally, in the production process of meat products in the field of meat processing, in the production of ham, sausage and the like, the meat color is fixed so as to give a unique reddish color to the meat product. In order to improve the storage property by suppressing the growth of microorganisms, 0.0 > nitrite < / RTI >

In other words, synthetic nitrite acts on myoglobin and hemoglobin, which are the coloring elements, to produce nitrosomoglobin and nitrosohemoglobin, which are responsible for the expression and stabilization of color, and Clostridium botulinum Inhibition of secretory toxin production, flavor impartation and inhibition of the occurrence of acidic patches.

Synthetic nitrite has these advantages, but its use is regulated because it is known to form nitrosoamines or cause methemoglobinemia in children or fetuses. Korea and Japan regulate the residual amount of synthetic nitrite in the final product to be less than 70 ppm, and most countries such as England and Germany also regulate the residual amount of synthetic nitrite to 10-200 ppm.

Particularly, in October 2015, IARC (International Cancer Research Institute) affiliated with WHO classified the processed meat such as ham and sausage as Group 1 carcinogen, after which consumers purchased a product without synthetic nitrite However, there is a problem that the color or flavor of the product is significantly lower than that of the product containing the synthetic nitrite.

In addition, nitrite itself shows toxicity, and when taken at a certain concentration or more, hemoglobin in the blood is oxidized to form methemoglobin, which can cause various addictive symptoms such as methemoglobinemia. Therefore, there is no commercially available technology to reduce the amount of synthetic nitrite in the world, but it is only a level of lowering the residual amount or adding substitutable materials by adding a functional material in the production of meat products.

Thus, there is a need to develop natural nitrite to replace the synthetic nitrite.

Korean Patent No. 308387 Korean Registered Patent No. 2006-17659

It is an object of the present invention to provide a process for producing natural nitrite which can replace synthetic nitrite used in meat products.

Another object of the present invention is to provide a natural nitrite prepared according to the process for producing the natural nitrite.

It is still another object of the present invention to provide a method of coloring raw meat using the natural nitrite.

It is still another object of the present invention to provide a meat product that has been developed in accordance with the coloring method.

In order to accomplish the above object, there is provided a method for producing natural nitrite, which is a coloring agent of the present invention, comprises the steps of: preparing a spinach extract using powder obtained by lyophilizing and spinning spinach; And adding the sugar and Lactobacillus farciminis ( KCTC 3618) to the spinach extract and fermenting at 20 to 35 ° C for 15 to 95 hours.

Further, after the fermentation step, the fermented spinach fermentation product may be irradiated with UV or pasteurized at a low temperature of 60 to 65 ° C.

The spinach extract may be an extract obtained by stirring the spinach powder with distilled water at 20 to 28 DEG C for 20 to 200 minutes.

The UV irradiation is for stopping the fermentation of the fermented product of spinach, and when the fermentation is not stopped, the produced natural nitrite may be lost by the microorganism.

The sugar can be sucrose or grape sugar.

In order to achieve the above-mentioned other objects, the natural nitrite of the present invention may be prepared according to the above-described method.

According to another aspect of the present invention, there is provided a method for coloring raw meat, comprising the steps of (A) 100 parts by weight of a raw material, 3 to 20 parts by weight of a natural nitrite prepared by the above method, and 90 to 200 parts by weight of a material Mixing; And (B) dewaxing the mixture.

Filling, shaping and heat treating said dirtied mixture; And cooling and cooling the heat-treated mixture.

The natural nitrite may be mixed in 5 to 10 parts by weight.

According to another aspect of the present invention, there is provided a meat product, which is colored according to a method of coloring the raw meat.

The meat product may be ham, bacon or sausage.

The natural nitrite (coloring agent) using the spinach extract of the present invention can be prepared by using Lactobacillus farciminis ( KCTC 3618) as a nitrate reducing bacterium, in the same manner as in the case of adding sodium nitrite without addition of synthetic sodium nitrite, It has flavor.

In addition, protein breakdown (VBN) and lipid rancidity (TBA) do not occur rapidly, and the residual nitrite content is much lower than when using synthetic sodium nitrite.

The present invention relates to a natural nitrite capable of replacing the synthetic nitrite used in meat products, and a method for coloring raw meat including the same.

Hereinafter, the present invention will be described in detail.

The method of producing natural nitrite of the present invention comprises the steps of: preparing a spinach extract using powder obtained by lyophilizing and spinning spinach; And fermenting the spinach extract with Lactobacillus farciminis ( KCTC 3618) for 15 to 95 hours at 20 to 35 ° C.

First, spinach extract is prepared by using spinach powder.

The spinach extract is prepared by lyophilizing a spinach having a nitrate level of 800 to 3700 ppm and pulverizing and pulverizing the mixture. The spinach powder and distilled water are mixed at a weight ratio of 1: 5 to 30, preferably 1: 8 to 12, Preferably at 23 to 26 DEG C for 20 to 200 minutes, preferably 30 to 100 minutes, more preferably 30 to 50 minutes at 50 to 200 rpm. In the case where the spinach powder is not lyophilized during the production, it is difficult to produce natural nitrite since the nitrate is lost.

In the present invention, when other vegetables such as lettuce, celery, and red beet are used instead of spinach, color development is poor, and VBN and TBA may be high. In addition, when extracted with an organic solvent such as ethanol, methanol or the like without extracting with distilled water, or when extract is used, the coloring ability is low and the deterioration can proceed quickly.

When the extraction temperature and time of the spinach extract are less than the lower limit, the coloring ability may not be excellent when used in the ginger base. If the extraction temperature and the extraction time of the spinach extract are higher than the upper limit, the breakage of the meat may progress rapidly.

Next, saccharide and lactobacillus farciminis ( KCTC 3618) are added to the spinach extract and fermented at 20 to 35 ° C for 15 to 95 hours to produce nitrite.

In the present invention, Lactobacillus farciminis ( KCTC 3618) is used as a nitrate reducing bacteria capable of reducing nitrate to nitrite. If Lactobacillus farciminis ( KCTC 3618) is replaced by staphylococcus genus, micrococcus genus, Enterococcus genus, Lactococcus genus, ), Streptococcus genus, Pediococcus genus, Leuconostoc genus, etc. are used, the reactivity with the spinach extract is lowered and the color development is poor, and VBN and TBA Can be high.

The Lactobacillus farciminis ( KCTC 3618) is used in an amount of 0.01 to 1 part by weight, preferably 0.02 to 0.05 part by weight based on 100 parts by weight of the spinach extract.

Further, the saccharide is one capable of improving the production amount of nitrite, and specifically may be sucrose or grape sugar. When fructose or lactose is used as the saccharide, it is preferable to use sucrose or glucose since nitrite similar to the case of not using saccharide is produced.

The sugar is used in an amount of 5 to 30 parts by weight, preferably 10 to 20 parts by weight, based on 100 parts by weight of the spinach extract. If the content of sugar is less than the lower limit, the content of nitrite may not be increased. If the content of sugar exceeds the upper limit, the content of nitrite may be rapidly lowered.

The fermentation of the present invention is fermented at 20 to 35 ° C, preferably 22 to 33 ° C, for 15 to 95 hours, preferably 24 to 72 hours. When the fermentation time and temperature are lower than the lower limit, the content of nitrite may be low and the rate of decay of meat product may be high. If the fermentation time and temperature are higher than the upper limit, the content and the functionality of nitrite may be lowered.

Next, the nitrite-generated spinach fermentation product is pasteurized for 30 to 60 minutes at a low temperature of 60 to 65 ° C or UV irradiation so as to control microorganisms. The UV irradiation or pasteurization is performed to terminate the fermentation for producing the nitrite. When the UV irradiation or pasteurization is not performed, the content of nitrite is continuously lowered through fermentation. In addition, when it is carried out under severe conditions such as LTLT (ultrahigh-temperature short-time heating) or retort instead of UV irradiation or pasteurization, the content of nitrite decreases.

The UV is preferably irradiated for 5 to 60 minutes, preferably 10 to 20 minutes.

In addition, the present invention provides a method of coloring raw meat including the natural nitrite.

The method for coloring the raw meat of the present invention comprises the steps of (A) mixing raw meat, natural nitrite as described above and a sub ingredient; (B) dewaxing the mixture; (C) filling, shaping and heat-treating the dirtied mixture; And (D) cooling and packing the heat-treated mixture.

First, in step (A), 3 to 20 parts by weight, preferably 5 to 10 parts by weight, and 90 to 200 parts by weight of the raw material nitrite prepared above are mixed in 100 parts by weight of the raw material.

When the content of the natural nitrite is less than the lower limit value, the VBN and the TBA value may not be developed and the VBN and TBA may be higher.

Examples of the above materials include, but are not limited to, sodium chloride and sodium tripolyphosphate.

Next, in the step (B), the mixture is dyed.

The dyed jelly means a process in which a mixed coloring agent and a raw material are sufficiently reacted with raw meat and naturally fermented nitrite fixes the pigment of the raw meat to form a poultry meat color and form a unique flavor and taste.

The dirt is to homogenize the mixture for 3 to 30 minutes while maintaining the temperature at 0 to 10 [deg.] C.

Next, in the step (C), the dirtied mixture is filled, formed and heat-treated.

The filling refers to the process of injection or vacuum sealing the mixture into an edible casing (e.g., intestine, collagen or gelatin), plastic (film) or can.

Further, the heat treatment is performed at 70 to 120 캜, preferably 80 to 90 캜, for 40 to 100 minutes, preferably 50 to 70 minutes.

Next, in the step (D), the heat-treated mixture is cooled and then packed.

The cooling is carried out by cooling the heat-treated mixture at 15 to 25 DEG C for 1 to 5 hours.

Examples of meat products produced by the above method include ham, bacon or sausage.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

<By type of vegetables>

[Examples and Comparative Examples]

Control group. Omit the coloring agent

Fresh pork meat is not slaughtered only after 48 hours (castrated boars, Yorkshire X Landrace X Duroc; approximately 110 kg, M. biceps femoris, M. semitendinosus, M. semimembranosus) and pork fat (moisture 12.61%, fat 85.64 %) Were prepared and all subcutaneous and intramuscular fat connected to the tissue were removed using a knife.

100 parts by weight of pork meat, 50 parts by weight of pork fat and 50 parts by weight of ice were homogenized for 1 minute and 30 seconds with an automatic cutter (Nr-963009; Hermann Scharfen GmbH & Co., Germany), 2 parts by weight of sodium chloride, 0.3 parts by weight of sodium phosphate was added and homogenized for 3 minutes to prepare meat products.

Example 1: Spinach extract + Lactobacillus parciminis ( Lactobacillus farciminis, KCTC 3618) + sucrose

Cultured spinach extract

The spinach was lyophilized for 12 hours, and the pulverized spinach powder and distilled water were mixed at a weight ratio of 1:10, followed by stirring at 25 ° C for 30 minutes to obtain a spinach extract.

To 100 parts by weight of the spinach extract, 0.025 part by weight of Lactobacillus farciminis ( KCTC 3618) and 10 parts by weight of sucrose were added and cultured at 30 DEG C for 36 hours. Then, the fermented product was irradiated with a UV light for 15 minutes, And evaporated with a rotary evaporator (EYELA N-1000, Rikakikai, Japan) to obtain a coloring agent as a culture spinach extract.

Meat products

Fresh pork meat is not slaughtered only after 48 hours (castrated boars, Yorkshire X Landrace X Duroc; approximately 110 kg, M. biceps femoris, M. semitendinosus, M. semimembranosus) and pork fat (moisture 12.61%, fat 85.64 %) Were prepared and all subcutaneous and intramuscular fat connected to the tissue were removed using a knife.

100 parts by weight of pork meat, 50 parts by weight of pork fat and 50 parts by weight of ice were homogenized for 1 minute and 30 seconds with an automatic cutter (Nr-963009; Hermann Scharfen GmbH & Co., Germany), 2 parts by weight of sodium chloride, 0.3 parts by weight of sodium phosphate and 6 parts by weight of the above-mentioned cultured spinach extract were added, and homogenized for 3 minutes to prepare meat products.

Comparative Example 1. Synthesis of nitrite

Fresh pork meat is not slaughtered only after 48 hours (castrated boars, Yorkshire X Landrace X Duroc; approximately 110 kg, M. biceps femoris, M. semitendinosus, M. semimembranosus) and pork fat (moisture 12.61%, fat 85.64 %) Were prepared and all subcutaneous and intramuscular fat connected to the tissue were removed using a knife.

100 parts by weight of pork meat, 50 parts by weight of pork fat and 50 parts by weight of ice were homogenized for 1 minute and 30 seconds with an automatic cutter (Nr-963009; Hermann Scharfen GmbH & Co., Germany), 2 parts by weight of sodium chloride, 0.3 parts by weight of sodium phosphate and 6 parts by weight of synthetic nitrite were added and homogenized for 3 minutes to prepare meat products.

Comparative Example  2. Lettuce Extract + Lactobacillus Parsiminis ( Lactobacillus farciminis , KCTC  3618) + sucrose

The extract was prepared in the same manner as in Example 1 except that lettuce powder was used instead of spinach powder.

Comparative Example  3. Celery extract + Lactobacillus Parsiminis ( Lactobacillus farciminis , KCTC  3618) + sucrose

The extract was prepared in the same manner as in Example 1 except that celery powder was used instead of spinach powder.

Comparative Example  4. Leaf leaf extract + Lactobacillus Parsiminis ( Lactobacillus farciminis , KCTC  3618) + sucrose

The extracts were prepared in the same manner as in Example 1 except that radish leaves were used instead of spinach powder.

Comparative Example  5. Extract of parsley + Lactobacillus Parsiminis ( Lactobacillus farciminis , KCTC  3618) + sucrose

The extract was prepared in the same manner as in Example 1 except that the powder was replaced with the powder of spinach, and a meat product was prepared using the extract.

[ Test Example _One]

Test Example  1. Measurement of pH, color and nitrite content of vegetable extracts

1-1. pH: Measured three times using a pH meter (Model 340, Mettler-Toledo GmbH, Switzerland).

1-2. Chroma: It was observed with a colorimeter (Chroma meter CR-210, Minolta, Japan) using a 8 mm diameter measuring area and a 50 mm diameter illuminating area. (Positive = yellow, negative = blue) values of L * (100 = white, 0 = black), a * (positive = red, negative = green) and b * (positive = yellow, negative = blue). The standard white plate was normalized to an L value of 97.12, a value of -0.13 and b value of 2.14.

1-3. Nitrite Concentration (ppm): The nitrite concentration was measured using the diazotization method.

end. reagent

(1) Ammonium acetate buffer

100 g of ammonium acetate is dissolved in about 900 ml of water, adjusted to pH 9.1 with 10% ammonia water, and water is added to make 1,000 ml.

(2) Sulfanilamide solution

Sulfanilamide (0.5 g) was dissolved in 100 ml of hydrochloric acid (1 + 1).

(3) Naphthylethylenediamine solution

0.12 g of N- (1-naphthyl) ethylenediamine hydrochloride is dissolved in 100 ml of water, and if there are impurities, the solution is filtered.

(4) Nitrite standard solution

Sodium nitrite (NaNO _{2 best available} product) is dried in a sulfuric acid desiccator for 24 hours, and 0.150 g is precisely weighed and dissolved in sterilized water to make 1,000 ml. Water is added to 10 ml of the standard stock solution to make 100 ml, and 2 ml of the solution is diluted to 100 ml with water to prepare a standard solution (prepared immediately before use).

1 ml of nitrite standard solution = 0.2 NO NO ₂

I. Preparation of Test Solution

Thoroughly weigh accurately about 10 g of the test sample, add an appropriate amount of water at about 80 ° C and mix evenly. This is quantitatively transferred to a 200 ml volumetric flask, the container is flushed several times with hot water and placed in a flask. The amount of liquid in the flask is about 150 ml. ★ Add 10 ml of 0.5 N sodium hydroxide solution, shake well, add 10 ml of 12% zinc sulfate solution, shake well, and heat in a water bath at 80 ℃ for 20 minutes with occasional shaking. Subsequently, it is immersed in cold water, cooled to room temperature, and then 20 ml of ammonium acetate buffer solution and water are added to make 200 ml. Mix the contents thoroughly, leave for 10 minutes, filter (using a drying filter paper), discard about 20 ml of the first filtrate, and take the clear filtrate into an Erlenmeyer flask to give a test solution. Separately, 10 ml of water is used in place of the test sample, and the solution prepared in the same manner as in the above ★ is used as a blank test solution.

All. Test Methods

Add 1 ml of the sulfanilamide solution to 20 ml of the test solution and blank test solution, and mix. Add 1 ml of a solution of naphthyl ethylenediamine and water to make 25 ml. Mix well. Allow to stand for 20 minutes. Measure the absorbance at 540 nm with absorbance Aa and Ab at the wavelength of 540 nm using 20 ml of water. When the test solution is colored, 1 ml of HCl (1 + 1) and water are added to 20 ml of the test solution to make 25 ml, and the absorbance Ac is measured using water as a reference solution. Determine the difference in absorbance Aa-Ab or Aa- (AbAc), calculate the amount of nitrite ion A (㎍) in 20 ml of test solution from the calibration curve prepared previously, and calculate the concentration of nitrite ions in the test sample according to the following equation.

[Equation 1]

S = amount of test sample (g)

Preparation of the calibration curve: 2, 5, 10, 15 and 20 ml of nitrite ion standard solution are taken in a 25 ml volumetric flask and each water is added to make about 20 ml. Add 1 ml of the sulfanilamide solution, add 1 ml of the naphthened ethylenediamine solution, add water to make 25 ml, and mix well. 20 ml of water is used as a reference solution and 20 minutes later, the absorbance is measured at a wavelength of 540 nm to prepare a calibration curve.

division powder extract Nitrate concentration (ppm) pH L * value a * value b * value Example 1 2174 5.24 52.43 -0.26 7.07 Comparative Example 2 2412 4.77 22.40 -2.03 20.31 Comparative Example 3 2440 4.31 15.21 4.19 19.66 Comparative Example 4 4875 4.68 37.56 -0.54 8.09 Comparative Example 5 478 4.93 30.21 -0.49 9.06

Test Example 2. Measurement of pH, Chromaticity, Tone Angle and Color Difference of Raw Meat Products

The pH and the chromaticity were the same as in Test Example 1 above.

The hue angle ( H ) and the color difference ( E ^* ) were calculated as shown in the following equations (2) and (3), respectively.

&Quot; (2) &quot;

&Quot; (3) &quot;

division pH L * value a * value b * value Hue Angle Color difference Control group 6.41 61.10 11.82 12.28 46.11 - Example 1 6.46 62.04 8.93 12.84 55.20 3.15 Comparative Example 1 6.48 62.10 10.05 11.63 49.17 2.24 Comparative Example 2 6.45 58.27 3.45 13.72 75.88 9.00 Comparative Example 3 6.02 61.25 8.23 15.13 58.62 3.95 Comparative Example 4 6.35 61.45 7.02 15.94 63.68 5.31 Comparative Example 5 6.40 57.69 4.16 14.85 70.71 8.84

As shown in Table 2 above, it was confirmed that the green product produced according to Example 1 of the present invention had brighter and more redness than the meat products of Comparative Examples 2 to 5.

The higher the value of the hue angle, the more brownish the product. The product of Example 1 had lower color tone and a lower color difference than the product of Comparative Examples 2 to 5.

Test Example  3. After cooking Meat product  pH, color, hue angle and Color difference  Measure

The pH, chromaticity, hue angle and color difference were measured in the same manner as in Test Example 2.

division pH L * value a * value b * value Hue Angle Color difference Control group 6.50 66.29 3.21 11.18 73.99 - Example 1 6.53 65.97 4.75 11.78 68.02 2.01 Comparative Example 1 6.55 66.05 9.81 9.46 43.99 6.84 Comparative Example 2 6.51 62.80 0.25 13.54 88.95 5.16 Comparative Example 3 6.40 63.54 3.81 13.06 73.73 2.15 Comparative Example 4 6.47 63.73 3.01 13.67 76.88 2.37 Comparative Example 5 6.50 60.42 1.68 12.18 80.68 5.11

As shown in Table 3 above, meat products prepared according to Example 1 of the present invention were found to be brighter and more excellent in redness than meat products of Comparative Examples 2 to 5.

In addition, it was confirmed that the green product of Example 1 had lower color tone and lower color difference than the meat products of Comparative Examples 2 to 5.

Test Example  4. After cooking Meat product VBN  And TBA measurements

VBN and TBA measurements were taken after cooking the cooked meat products at 5 ° C for 10 days.

4.1 Protein Degradation (VBN: Volatile Basic Nitrogen): 5 g of sample was added with 50 g of distilled water, homogenized at 13,500 rpm for 1 min, and filtered through a filter paper (Whatman No. 1). Then, 50 μl of 0.01NH ₃ BO ₃ and 0.06% methyl red in ethanol (0.066% bromocresol green in ethanol = 1: 1) were added to the inner chamber using a conway vessel, Ml, and 1 ml of 50% K ₂ CO ₃ was added thereto, followed by quick sealing. Then, the reaction was carried out in a 37 ° C incubator for 90 minutes and titrated rapidly with 0.02 NH ₂ SO ₄ . The ball was not loaded with K ₂ CO ₃ . The degree of protein deterioration was calculated according to the following equation (4).

&Quot; (4) &quot;

VBN (mg%) = {(a-b) x f x 0.02 x 14.007} / S x 100

(ml), (b): (ml), (f): 0.02NH ₂ SO ₄ standardization index, and S: sample weight)

4.2 Fat Oxidation (TBA) (mg MA / kg): Lipid oxidation was performed by Tarladgis et al . (1960), and expressed as mg of malondialdehyde (MD) per kg of meat product. 50 ml of distilled water was added to 10 g of the sample, and the mixture was mixed with a homogenizer (AM-7, Nihonseiki, Kaisha Ltd., Japan) for 2 minutes. 47.5 ml of distilled water was then added, followed by 2.5 ml of 4N HCl, -73, Shin-Etsu Silicone Co., Ltd., Seoul, Korea). The mixture was distilled and 50 ml of distillate was collected.

5 ml of 0.02 M TBA (TBA reagent) in 90% acetic acid was added to a test tube containing 5 ml of distilled water and mixed. The tube was sealed, heated in boiling water for 30 minutes, and cooled to room temperature. Absorbance was measured at 538 nm for a blank made from 5 ml distilled water and 5 ml TBA reagent using a UV / VIS spectrophotometer (Optizen 2120 UV plus, Mecasys Co. Ltd., Seoul, South Korea).

division VBN (mg%) TBA (mg MA / kg) Control group 3.79 0.39 Example 1 2.33 0.16 Comparative Example 1 1.76 0.06 Comparative Example 2 5.26 0.22 Comparative Example 3 2.83 0.36 Comparative Example 4 3.19 0.39 Comparative Example 5 3.93 0.41

As shown in Table 4, the meat products prepared according to Example 1 of the present invention had lower VBN and TBA values than those of the control and the meat products of Comparative Examples 2 to 5.

This means that the meat product of Example 1 inhibits enzymes and microorganisms as compared with the meat products of the control and Comparative Examples 2 to 5, resulting in a slow deterioration.

Test Example  5. After cooking Meat product  Microbiological measurement

Microbiological measurements were made after storing the cooked meat products at 5 ° C for 10 days.

The 25 g sample was homogenized for 3 minutes and then transferred to a stomacher bag (IUL Instrument, Barcelona, Spain), diluted with 225 ml of 0.1% peptone aqueous solution, and 1 ml of the diluted sample was placed in a Petri dish Respectively. 20 ml of a plate count agar (PCA; Difco, Sparks, MD, USA) was applied onto the plate containing the diluted sample. After the medium solidified, the plate was incubated at 37 ° C for 48 hours, Were measured.

division Total number of bacteria E. coli (E. coli) Coliform bacteria Control group 2.10 N.D. N.D. Example 1 1.42 N.D. N.D. Comparative Example 1 1.61 N.D. N.D. Comparative Example 2 1.68 N.D. N.D. Comparative Example 3 1.64 N.D. N.D. Comparative Example 4 1.69 N.D. N.D. Comparative Example 5 1.68 N.D. N.D.

As shown in Table 5 above, E. coli and E. coli bacteria were not detected in all the groups, and meat products prepared according to Example 1 of the present invention were cooked in a total amount, as compared with the control group and Comparative Examples 1 to 5 And the number of bacteria was low.

Test Example 6. Measurement of residual nitrite in cooked meat product

Residual nitrite content was measured according to AOAC (1990) and expressed in ppm per kg of sample. All residual nitrite analysis was performed in duplicate, and all treatments were analyzed simultaneously to minimize bias in analysis at expiration time. The residual nitrite content was calculated using a nitrite solution (KFDA, 2013).

division Control group Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Residual nitrite
(ppm) 0.0 12.4 60.5 25.9 20.8 26.7 27.9

As shown in Table 6 above, the meat products prepared according to Example 1 of the present invention were found to have a lower residual nitrite content than Comparative Examples 1 to 5. Comparative Example 1 is the remaining amount of the synthesized nitrite.

In particular, the meat product of Example 1 was found to have significantly lower nitrite content than the meat product of Comparative Example 1 using synthetic nitrite.

Even if it originates in nature, there is a problem that nitrite causes cancer and the like. When the spinach extract of the present invention is used, the content of the residual nitrite is remarkably lowered.

Test Example  7. After cooking Meat product  Sensory test

The meat products obtained by cooking the meat products prepared in the examples and the comparative examples were sampled by 10 special panelists and then subjected to sensory evaluation by the 9-point scale method. The average value of the meat products was shown in Table 7 below.

- Appearance, taste, incense, texture and comprehensive preference: 1 point = very bad, 9 points = very good

division Exterior flavor incense Texture Comprehensive likelihood Control group 3.1 3.0 3.9 3.7 3.0 Example 1 7.9 8.1 8.2 8.3 8.3 Comparative Example 1 8.3 8.1 8.6 8.7 8.5 Comparative Example 2 3.2 3.5 3.9 3.4 3.4 Comparative Example 3 4.3 4.0 4.6 4.3 4.5 Comparative Example 4 4.1 3.6 4.5 4.0 4.2 Comparative Example 5 4.0 3.4 4.9 3.8 3.9

As shown in Table 7 above, meat products prepared according to Example 1 of the present invention had excellent appearance, taste, flavor, texture, and overall acceptability compared to the control and Comparative Examples 2 to 5 Respectively.

In addition, it was confirmed that the meat product of Example 1 showed similar functionality to the meat product of Comparative Example 1 using the synthetic nitrite.

As a result of experiments with other vegetables with similar or higher nitrate concentrations than those of spinach, it was found that nitrate concentrations were similar, higher or lower than those of spinach, but were not superior in color, deterioration, residual nitrite concentration and sensory properties Respectively.

<Nitrate Reducing bacteria  Category>

Control group. Omit the coloring agent

The control group was used.

Example  1. Spinach extract + Lactobacillus Parsiminis ( Lactobacillus farciminis , KCTC  3618) + sucrose

The above Example 1 was used.

Comparative Example  6. Spinach extract + Staphylococcus Carnosus staphylococcus carnosus ) + Sucrose

The extract was prepared by using staphylococcus carnosus instead of Lactobacillus farciminis ( KCTC 3618), and the meat products were prepared using the extracts in the same manner as in Example 1, except that Lactobacillus farciminis ( KCTC 3618) was used instead of Lactobacillus farciminis ( KCTC 3618).

Comparative Example  7. Spinach Extract + Staphylococcus Bitorinus staphylococcus vitulinus ) + Sucrose

The extract was prepared in the same manner as in Example 1 except that staphylococcus vitulinus was used instead of Lactobacillus farciminis ( KCTC 3618) , and meat products were prepared using the extract.

Comparative Example  8. Spinach Extract + Staphylococcus Xylosus (Staphylococcus xylosus ) + Sucrose

Except that Staphylococcus xylosus was used instead of Lactobacillus farciminis ( KCTC 3618), and a meat product was prepared using the extracts as described in Example 1, except that Lactobacillus farciminis ( KCTC 3618) was used instead of Lactobacillus farciminis .

[ Test Example _2]

Test Example  8. Raw raw Meat product  pH, color, hue angle and Color difference  Measure

The pH, chromaticity, hue angle and color difference were measured in the same manner as in Test Example 2.

division pH L * value a * value b * value Hue Angle Color difference Control group 6.41 61.10 11.82 12.28 46.11 - Example 1 6.46 62.04 8.93 12.84 55.20 3.15 Comparative Example 6 6.41 59.41 7.36 15.46 59.67 4.93 Comparative Example 7 6.40 56.15 6.14 14.93 63.71 5.87 Comparative Example 8 6.35 58.42 6.92 15.82 58.99 5.68

As shown in Table 8 above, it was confirmed that the green products produced according to Example 1 of the present invention are brighter in brightness and higher in redness than the meat products of Comparative Examples 6 to 8.

In addition, it was confirmed that the green product of Example 1 had lower hue angle and color difference than the meat products of Comparative Examples 6 to 8.

Test Example 9. pH, color, hue angle and color difference measurement of the meat product after cooking

The pH, chromaticity, hue angle and color difference were measured in the same manner as in Test Example 2.

division pH L * value a * value b * value Hue Angle Color difference Control group 6.50 66.29 3.21 11.18 73.99 - Example 1 6.53 65.97 4.75 11.78 68.02 2.01 Comparative Example 6 6.47 61.49 2.94 15.67 76.84 4.81 Comparative Example 7 6.35 57.62 1.53 15.84 70.61 5.09 Comparative Example 8 6.42 58.75 2.45 15.77 73.70 4.62

As shown in Table 9 above, meat products prepared according to Example 1 of the present invention were found to be brighter in brightness and better in redness than meat products of Comparative Examples 6 to 8.

It was also confirmed that the meat products of Example 1 had lower hue angles and color differences than the meat products of Comparative Examples 6 to 8.

Test Example 10. Measurement of VBN and TBA of cooked meat products

VBN and TBA were measured in the same manner as in Test Example 4.

division VBN (mg%) TBA (mg MA / kg) Control group 3.79 0.39 Example 1 2.33 0.16 Comparative Example 6 3.84 0.37 Comparative Example 7 3.91 0.42 Comparative Example 8 3.89 0.40

As shown in Table 10, it was confirmed that the meat products prepared according to Example 1 of the present invention had lower VBN and TBA values than those of the control and the meat products of Comparative Examples 6 to 8.

Test Example  11. After cooking Meat product  Measurement of residual nitrite

The residual nitrite was conducted in the same manner as in Test Example 6.

division Control group Example 1 Comparative Example 6 Comparative Example 7 Comparative Example 8 Residual nitrite
(ppm) 0.0 12.4 34.2 36.9 35.9

As shown in Table 11, it was confirmed that the meat product prepared according to Example 1 of the present invention had a lower residual nitrite content than Comparative Examples 6 to 8.

Test Example 12. Sensory evaluation of meat products after cooking

The functionalities were the same as in Test Example 7.

division Exterior flavor incense Texture Comprehensive likelihood Control group 3.1 3.0 3.9 3.7 3.0 Example 1 7.9 8.1 8.2 8.3 8.3 Comparative Example 6 4.2 3.8 4.0 6.7 6.3 Comparative Example 7 4.5 4.2 4.5 6.9 6.5 Comparative Example 8 4.2 4.3 4.3 6.8 6.3

As shown in Table 12 above, meat products prepared according to Example 1 of the present invention were found to have excellent appearance, taste, flavor, texture, and overall acceptability in comparison with Comparative Examples 6 to 8.

As a result of the microorganism measurement, E. coli and E. coli bacteria were also not detected in Comparative Examples 6 to 8. However, It was confirmed that the total number of bacteria was higher than that of the meat product of Example 1.

<By extraction solvent>

[ Example  And Comparative Example ]

Control group. Omit the coloring agent

The control group was used.

Example  1. Spinach water extract + Lactobacillus Parsiminis ( Lactobacillus farciminis , KCTC  3618) + sucrose

The above Example 1 was used.

Comparative Example  9. Spinach Heat number  Extract + Lactobacillus Parsiminis ( Lactobacillus farciminis , KCTC  3618) + sucrose

Instead of extracting the spinach powder with distilled water at 25 ° C, the spinach powder was extracted with distilled water 100 to prepare a hot water extract.

Comparative Example  10. Spinach ethanol extract + Lactobacillus Parsiminis ( Lactobacillus farciminis , KCTC  3618) + sucrose

Instead of extracting the spinach powder with distilled water at 25 ° C, the spinach powder was extracted with 80% ethanol 25 to prepare a meat product.

Comparative Example  11. Spinach juice + Lactobacillus Parsiminis ( Lactobacillus farciminis , KCTC  3618) + sucrose

The same procedure as in Example 1 was carried out except that the spinach powder was extracted with distilled water at 25 ° C, and a spinach juice obtained by spinach spinach was used to produce meat products.

[ Test Example _3]

Test Example  13. Raw raw Meat product  pH, color, hue angle and color

The pH, chromaticity, hue angle and color difference were measured in the same manner as in Test Example 2.

division pH L * value a * value b * value Hue Angle Color difference Control group 6.41 61.10 11.82 12.28 46.11 - Example 1 6.46 62.04 8.93 12.84 55.20 3.15 Comparative Example 9 6.47 54.38 5.80 15.96 61.45 7.39 Comparative Example 10 6.49 58.12 6.58 16.11 59.37 6.17 Comparative Example 11 6.45 51.69 4.10 15.87 63.72 8.95

As shown in Table 13 above, it was confirmed that the green product produced according to Example 1 of the present invention had a brighter brightness and a better redness than the meat products of Comparative Examples 9 to 11.

It was also confirmed that the green product of Example 1 had lower hue angle and color difference than the meat products of Comparative Examples 9 to 11.

Test Example  14. After cooking Meat product  pH, color, hue angle and Color difference  Measure

The pH, chromaticity, hue angle and color difference were measured in the same manner as in Test Example 2.

division pH L * value a * value b * value Hue Angle Color difference Control group 6.50 66.29 3.21 11.18 73.99 - Example 1 6.53 65.97 4.75 11.78 68.02 2.01 Comparative Example 9 6.49 59.47 2.46 15.49 77.35 5.09 Comparative Example 10 6.50 60.43 2.69 15.31 75.19 5.88 Comparative Example 11 6.47 58.19 1.98 14.59 80.47 6.11

As shown in Table 14, it was confirmed that the meat products prepared according to Example 1 of the present invention were superior in brightness and redness to the meat products of Comparative Examples 9 to 11.

It was also confirmed that the meat products of Example 1 had lower hue angles and color differences than the meat products of Comparative Examples 9 to 11.

Test Example 15. Measurement of VBN and TBA of cooked meat products

VBN and TBA were measured in the same manner as in Test Example 4.

division VBN (mg%) TBA (mg MA / kg) Control group 3.79 0.39 Example 1 2.33 0.16 Comparative Example 9 3.68 0.38 Comparative Example 10 3.42 0.30 Comparative Example 11 3.91 0.43

As shown in Table 15, it was confirmed that the meat products prepared according to Example 1 of the present invention had lower VBN and TBA values than those of the control and the meat products of Comparative Examples 9 to 11.

Test Example  16. After cooking Meat product  Measurement of residual nitrite

The residual nitrite was conducted in the same manner as in Test Example 6.

division Control group Example 1 Comparative Example 9 Comparative Example 10 Comparative Example 11 Residual nitrite
(ppm) 0.0 12.4 39.5 35.8 43.2

As shown in Table 16 above, the meat products prepared according to Example 1 of the present invention were found to have a lower residual nitrite content than Comparative Examples 9 to 11.

Test Example 17: Sensory evaluation of meat products after cooking

The functionalities were the same as in Test Example 7.

division Exterior flavor incense Texture Comprehensive likelihood Control group 3.1 3.0 3.9 3.7 3.0 Example 1 7.9 8.1 8.2 8.3 8.3 Comparative Example 9 3.8 3.8 3.7 4.0 3.8 Comparative Example 10 3.9 3.4 3.1 4.5 4.0 Comparative Example 11 3.1 3.2 4.3 3.7 3.3

As shown in Table 17, it was confirmed that meat products prepared according to Example 1 of the present invention had excellent appearance, taste, flavor, texture, and overall acceptability in comparison with Comparative Examples 9 to 11.

As a result of the measurement of microorganisms, neither Escherichia coli nor Escherichia coli bacteria were detected in Comparative Examples 9 to 11. However, It was confirmed that the total number of bacteria was higher than that of the meat product of Example 1.

<By type of party>

[Examples and Comparative Examples]

Control group. Omit the coloring agent

The control group was used.

Example 1: Spinach extract + Lactobacillus parciminis ( Lactobacillus farciminis, KCTC 3618) + sucrose

The above Example 1 was used.

Example 2: Spinach extract + Lactobacillus parciminis ( Lactobacillus farciminis, KCTC 3618) + glucose

The extract was prepared in the same manner as in Example 1 except that glucose was used instead of sucrose and meat products were prepared using the extract.

Comparative Example 12. Spinach Extract + Lactobacillus Parsiminis ( Lactobacillus farciminis, KCTC 3618) + fructose

The extract was prepared in the same manner as in Example 1 except that fructose was used instead of sucrose and meat products were prepared using the extract.

Comparative Example 13. Spinach Extract + Lactobacillus Parsiminis ( Lactobacillus farciminis, KCTC 3618) + lactose

The extract was prepared in the same manner as in Example 1 except that lactose was used instead of sucrose, and meat products were prepared using the extract.

[Test Example _4]

Test Example 18. pH, color, hue angle and color difference measurement of raw meat product

The pH, chromaticity, hue angle and color difference were measured in the same manner as in Test Example 2.

division pH L * value a * value b * value Hue Angle Color difference Control group 6.41 61.10 11.82 12.28 46.11 - Example 1 6.46 62.04 8.93 12.84 55.20 3.15 Example 2 6.45 61.34 8.16 13.02 56.09 3.39 Comparative Example 12 6.48 56.73 5.83 16.12 61.30 6.72 Comparative Example 13 6.43 57.35 6.10 15.77 62.78 6.59

As shown in Table 18 above, it was confirmed that the green products produced according to Examples 1 and 2 of the present invention are brighter in brightness and higher in redness than the meat products of Comparative Examples 12 to 13.

It was also confirmed that the green products of Examples 1 and 2 had lower hue angles and color differences than the meat products of Comparative Examples 12 to 13.

Test Example  19. After cooking Meat product  pH, color, hue angle and Color difference  Measure

The pH, chromaticity, hue angle and color difference were measured in the same manner as in Test Example 2.

division pH L * value a * value b * value Hue Angle Color difference Control group 6.50 66.29 3.21 11.18 73.99 - Example 1 6.53 65.97 4.75 11.78 68.02 2.01 Example 2 6.49 65.10 4.38 12.27 68.59 2.41 Comparative Example 12 6.48 56.17 2.68 15.68 77.81 5.88 Comparative Example 13 6.50 58.49 3.03 15.33 75.43 5.19

As shown in Table 19 above, meat products prepared according to Examples 1 and 2 of the present invention were found to be brighter and more excellent in redness than meat products of Comparative Examples 12 to 13.

It was also confirmed that the meat products of Examples 1 and 2 had lower hue angles and color differences than the meat products of Comparative Examples 12 to 13.

Test Example 20. Measurement of VBN and TBA of cooked meat products

VBN and TBA were measured in the same manner as in Test Example 4.

division VBN (mg%) TBA (mg MA / kg) Control group 3.79 0.39 Example 1 2.33 0.16 Example 2 2.56 0.18 Comparative Example 12 3.41 0.30 Comparative Example 13 3.20 0.26

As shown in Table 20 above, the meat products prepared according to Examples 1 and 2 of the present invention were found to have lower values of VBN and TBA than the meat products of the control and Comparative Examples 12 to 13 .

Test Example  21. After cooking Meat product  Measurement of residual nitrite

The residual nitrite was conducted in the same manner as in Test Example 6.

division Control group Example 1 Example 2 Comparative Example 12 Comparative Example 13 Residual nitrite
(ppm) 0.0 12.4 13.1 20.4 19.5

As shown in Table 21, the meat products prepared according to Examples 1 and 2 of the present invention were found to have a lower residual nitrite content than Comparative Examples 12 to 13.

Test Example 22. Sensory Evaluation of Meat Products after Cooking

The functionalities were the same as in Test Example 7.

division Exterior flavor incense Texture Comprehensive likelihood Control group 3.1 3.0 3.9 3.7 3.0 Example 1 7.9 8.1 8.2 8.3 8.3 Example 2 7.5 7.6 7.7 8.2 7.9 Comparative Example 12 6.1 6.0 7.3 7.8 7.3 Comparative Example 13 6.3 6.2 7.2 7.9 7.4

As shown in Table 22, the meat products prepared according to the examples 1 and 2 of the present invention had excellent appearance, taste, flavor, texture, and overall acceptability as compared with the comparative examples 12 to 13 Respectively.

As a result of the microorganism measurement, E. coli and E. coli bacteria were also not detected in Example 2 and Comparative Examples 12 to 13. However, It was confirmed that the meat products of Comparative Examples 12 to 13 had higher total bacteria than the meat products of Examples 1 and 2.

<By fermentation time and temperature>

[ Example  And Comparative Example ]

Control group. Omit the coloring agent

The control group was used.

Example  1. Spinach extract (30 ℃, 36 hours)

The above Example 1 was used.

Example  3. Spinach extract (30 ℃, 72 hours)

The extract was prepared in the same manner as in Example 1 except that the spinach extract and Lactobacillus parciminis cultured for 72 hours.

Example  4. Spinach extract (24 ℃, 36 hours)

The extract was prepared in the same manner as in Example 1 except that the spinach extract and Lactobacillus parciminis were cultivated at a temperature of 24 ° C.

Comparative Example  14. Spinach extract (30 ℃, 18 hours)

The extract was prepared in the same manner as in Example 1 except that the spinach extract and Lactobacillus parciminis cultured for 18 hours to prepare meat products.

Comparative Example  15. Spinach extract (30 ℃, 96 hours)

The extracts were prepared in the same manner as in Example 1 except that the spinach extract and Lactobacillus parciminis cultured for 96 hours.

Comparative Example  16. Spinach extract (36 ℃, 36 hours)

The extract was prepared in the same manner as in Example 1 except that the spinach extract and Lactobacillus parciminis were cultured at a temperature of 36 째 C to prepare a meat product.

[ Test Example _5]

Test Example  23. Pre- Meat product  pH, color, hue angle and Color difference  Measure

The pH, chromaticity, hue angle and color difference were measured in the same manner as in Test Example 2.

division pH L * value a * value b * value Hue Angle Color difference Control group 6.41 61.10 11.82 12.28 46.11 - Example 1 6.46 62.04 8.93 12.84 55.20 3.15 Example 3 6.42 61.29 8.51 12.71 55.98 3.26 Example 4 6.45 60.89 7.89 12.66 56.37 3.41 Comparative Example 14 6.42 43.12 5.03 14.36 62.99 6.81 Comparative Example 15 6.43 56.87 6.39 15.10 60.17 5.44 Comparative Example 16 6.47 53.71 5.97 14.99 61.54 5.71

As shown in Table 23 above, it was confirmed that the green products produced according to Examples 1, 3 and 4 of the present invention had higher brightness and better redness than the meat products of Comparative Examples 14 to 16.

It was also confirmed that the green products of Examples 1, 3 and 4 had lower hue angles and color differences than the meat products of Comparative Examples 14 to 16.

Test Example 24. pH, color, hue angle and color difference measurement of the meat product after cooking

The pH, chromaticity, hue angle and color difference were measured in the same manner as in Test Example 2.

division pH L * value a * value b * value Hue Angle Color difference Control group 6.50 66.29 3.21 11.18 73.99 - Example 1 6.53 65.97 4.75 11.78 68.02 2.01 Example 3 6.50 65.01 4.51 11.94 68.37 2.38 Example 4 6.51 64.52 4.00 12.05 68.98 2.71 Comparative Example 14 6.49 55.13 2.18 15.39 80.46 6.38 Comparative Example 15 6.50 59.35 3.47 16.17 73.69 5.17 Comparative Example 16 6.48 57.68 2.90 15.92 77.58 5.86

As shown in Table 24 above, meat products prepared according to Examples 1, 3 and 4 of the present invention were found to be brighter in brightness and better in redness than meat products of Comparative Examples 14 to 16 .

It was also confirmed that the meat products of Examples 1, 3 and 4 had lower hue angles and color differences than the meat products of Comparative Examples 14 to 16.

Test Example  25. After cooking Meat product VBN  And TBA measurements

VBN and TBA were measured in the same manner as in Test Example 4.

division VBN (mg%) TBA (mg MA / kg) Control group 3.79 0.39 Example 1 2.33 0.16 Example 3 2.51 0.18 Example 4 2.64 0.19 Comparative Example 14 4.67 0.30 Comparative Example 15 3.69 0.24 Comparative Example 16 4.15 0.28

As shown in Table 25, the meat products prepared according to Examples 1, 3 and 4 of the present invention were lower in VBN and TBA values than those of the control and the meat products of Comparative Examples 14 to 16 Respectively.

Test Example 26. Residual nitrite measurement of meat products after cooking

The residual nitrite was conducted in the same manner as in Test Example 6.

division Control group Example 1 Example 3 Example 4 Comparative Example 14 Comparative Example 15 Comparative Example 16 Residual nitrite
(ppm) 0.0 12.4 12.8 13.5 21.1 25.4 22.1

As shown in Table 26, it was confirmed that the meat products prepared according to Examples 1, 3 and 4 of the present invention had a lower residual nitrite content than Comparative Examples 14 to 16.

Test Example  27. After cooking Meat product  Sensory test

The functionalities were the same as in Test Example 7.

division Exterior flavor incense Texture Comprehensive likelihood Control group 3.1 3.0 3.9 3.7 3.0 Example 1 7.9 8.1 8.2 8.3 8.3 Example 3 7.5 7.8 8.0 8.1 8.1 Example 4 7.8 7.7 7.9 8.0 7.9 Comparative Example 14 5.1 6.3 6.5 6.4 6.4 Comparative Example 15 6.8 6.9 7.0 7.2 6.8 Comparative Example 16 6.0 6.1 6.7 6.5 6.4

As shown in Table 27 above, the meat product prepared according to the examples 1, 3 and 4 of the present invention had better appearance, taste, smell, texture and overall acceptability than the comparative examples 14 to 16 Respectively.

As a result of the microorganism measurement, E. coli and E. coli bacteria were also not detected in Examples 3 and 4 and Comparative Examples 14 to 16. However, It was confirmed that the meat products of Comparative Examples 14 to 16 had a higher total number of bacteria than the meat products of Examples 1, 3 and 4.

<By colorant content>

[ Example  And Comparative Example ]

Control group. Omit the coloring agent

The control group was used.

Example  1. Color developer 6 Weight portion

The above Example 1 was used.

Example  5. Colorant 15 Weight portion

The same procedure as in Example 1 was carried out except that 15 parts by weight of a coloring agent was used instead of 6 parts by weight to prepare meat products.

Comparative Example  17. Coloring Agent 25 Weight portion

The same procedure as in Example 1 was carried out except that 25 parts by weight of a coloring agent was used instead of 6 parts by weight to prepare a meat product.

Comparative Example  18. Color developer 1 Weight portion

The procedure of Example 1 was repeated except that 6 parts by weight of a coloring agent was used in an amount of 1 part by weight.

[ Test Example _6]

Test Example  28. After cooking Meat product  pH, color, hue angle and Color difference  Measure

The pH, chromaticity, hue angle and color difference were measured in the same manner as in Test Example 2.

division pH L * value a * value b * value Hue Angle Color difference Control group 6.50 66.29 3.21 11.18 73.99 - Example 1 6.53 65.97 4.75 11.78 68.02 2.01 Example 5 6.51 64.12 4.00 12.14 68.97 2.61 Comparative Example 17 6.50 57.13 3.11 15.88 76.84 5.88 Comparative Example 18 6.52 51.46 1.04 15.97 82.96 7.16

As shown in Table 28, the meat products prepared according to Examples 1 and 5 of the present invention were found to be brighter and more excellent in redness than the meat products of Comparative Examples 17 to 18.

It was also confirmed that the meat products of Examples 1 and 5 were lower in hue angle and color difference than the meat products of Comparative Examples 17 to 18.

Test Example 29. Measurement of VBN and TBA of cooked meat products

VBN and TBA were measured in the same manner as in Test Example 4.

division VBN (mg%) TBA (mg MA / kg) Control group 3.79 0.39 Example 1 2.33 0.16 Example 5 2.89 0.20 Comparative Example 17 5.14 0.39 Comparative Example 18 6.89 0.51

As shown in Table 29, it was confirmed that the meat products prepared according to Examples 1 and 5 of the present invention had lower VBN and TBA values than those of the control and the meat products of Comparative Examples 17 to 18 .

Test Example  30. After cooking Meat product  Measurement of residual nitrite

The residual nitrite was conducted in the same manner as in Test Example 6.

division Control group Example 1 Example 5 Comparative Example 17 Comparative Example 18 Residual nitrite
(ppm) 0.0 12.4 14.15 31.48 9.8

As shown in Table 30, it was confirmed that the meat product prepared according to Examples 1 and 5 of the present invention had a lower residual nitrite content than Comparative Example 17. In Comparative Example 18, the content of residual nitrite was low by using a small amount of the color developer itself.

Test Example 31. Sensory evaluation of meat products after cooking

The functionalities were the same as in Test Example 7.

division Exterior flavor incense Texture Comprehensive likelihood Control group 3.1 3.0 3.9 3.7 3.0 Example 1 7.9 8.1 8.2 8.3 8.3 Example 5 6.5 7.5 7.6 7.7 7.6 Comparative Example 17 4.6 5.1 5.4 5.5 5.6 Comparative Example 18 2.9 3.9 3.5 3.9 3.4

As shown in Table 31, the meat products prepared according to Examples 1 and 5 of the present invention had excellent appearance, taste, flavor, texture, and overall acceptability compared to Comparative Examples 17 to 18 Respectively.

As a result of the microorganism measurement, E. coli and E. coli bacteria were also not detected in Example 5 and Comparative Examples 17 to 18. However, It was confirmed that the meat products of Comparative Examples 17 to 18 had a higher total bacterial count than the meat products of Examples 1 and 5.

Claims (8)

Preparing a spinach extract by using a powder obtained by lyophilizing and spinning spinach; And
Adding lactose and Lactobacillus farciminis ( KCTC 3618) to the spinach extract, and fermenting the mixture at 20 to 35 ° C for 15 to 95 hours. (2). The method of claim 1, further comprising, after the step of fermenting, fermenting the fermented spinach fermentation product by UV irradiation or pasteurizing the fermented spinach fermentation product at a low temperature of 60 to 65 ° C. A process for the preparation of natural curing. The method according to claim 1, wherein the spinach extract is an extract obtained by mixing spinach powder and distilled water and stirring at 20 to 28 ° C for 20 to 200 minutes at 50 to 200 rpm. Gt; The method of claim 1, wherein the saccharide is sucrose or glucose. A natural nitrite utilizing bioconversion technology produced according to the process of any one of claims 1 to 4. (A) mixing 100 parts by weight of raw material, 3 to 20 parts by weight of natural nitrite produced by the manufacturing method of claim 1 and 90 to 200 parts by weight of the material; And
(B) dewatering the mixture. &Lt; Desc / Clms Page number 20 &gt; 7. The method of claim 6, further comprising: filling, shaping, and heat treating the salified mixture; And cooling and cooling the heat-treated mixture, and packaging the raw material. 7. Meat product developed according to the method of claim 6 or 7.