KR20180029613A - method for producing amino acids from animal raw blood - Google Patents

Abstract

The present invent invention relates to a method for generating an amino acid from a livestock blood. More specifically, the present invention relates to a method for generating an amino acid which sequentially carries out a proteolytic by a proteolytic enzyme and a peptidolytic by a peptidolytic enzyme with a separate process and significantly improves a production yield of an amino acid instead of simultaneously adding the proteolytic enzyme and the peptidolytic enzyme and degrading protein into a single process. The method for generating an amino acid from a livestock blood comprises: a collection step; a pulverization step; a proteolytic step; a solid-liquid separation step; and a sterilization treatment step.

Description

Methods for producing amino acids from animal blood from animal blood raw blood

The present invention relates to a method for producing amino acids in livestock blood, and more particularly, to a method for producing amino acids from livestock blood, The present invention relates to an amino acid production method capable of progressing peptide degradation by a degradation enzyme sequentially in separate processes, thereby greatly improving the yield of amino acid production.

The blood of livestock (pigs, cows, chickens, ducks, etc.) is a livestock by-product produced from slaughterhouses and is a useful resource rich in various proteins.

In Korea, the blood of these livestock is not used as a resource but is mostly wasted, which is a waste of useful protein resources as well as an environmental harmful factor.

Currently, livestock blood is mainly used for the production of animal protein feeds. It is supplied as a dried and powdered blood meal after livestock blood from slaughterhouses is collected. And is used as a raw material for pharmaceuticals.

The livestock blood is rich in various proteins, nutrients and minerals. Particularly, since the plasma part contains protein components such as albumin and globulin, and the hemocyte part contains a large amount of the protein component called hemoglobin, if the protein components can be decomposed and treated by a biological or chemical method, There is a possibility that they can be recovered.

However, in Korea, the waste blood of livestock is not recycled as a resource, and almost all of it is simply disposed of. Therefore, waste of useful resources and major cause of environmental pollution are causing serious problems.

In recent years, there has been a move toward recognition as a useful resource that can be recycled, away from the conventional view that the waste livestock blood is regarded merely as waste.

Korean Patent No. 10-135614 discloses a method for producing amino acids from raw blood of livestock.

The method of producing amino acids described above includes a pretreatment step of spraying Hwang Kuk-gyun on a blood-producing paper of a slaughterhouse and a passage through which the blood moves, a collection step of collecting blood generated in a slaughterhouse, And decomposing the protein in the blood by adding proteolytic enzyme to the blood.

In the conventional methods for producing amino acids, a protease is decomposed into amino acids through a single process by mixing a protease and a peptide decomposing enzyme. However, since two different enzymes collide with each other to inhibit protein degradation, There is a problem that the yield is low.

Korean Patent No. 10-135614: Method for producing amino acid from raw blood of livestock

The present invention has been made to overcome the above-mentioned problems, and it is an object of the present invention to provide a method and an apparatus for decomposing a protein by proteolytic enzymes and peptidolysis by a peptide degrading enzyme, not by decomposing a protein into a single process by simultaneously adding a proteolytic enzyme and a peptide- And a method for producing an amino acid capable of greatly increasing the yield of amino acid production.

In order to accomplish the above object, there is provided a method for producing amino acids in livestock blood, comprising the steps of: collecting blood of livestock generated in a slaughterhouse; A pulverizing step of pulverizing the by-products contained in the blood collected in the collecting step; A protein decomposition step of adding an enzyme to blood after completion of the pulverization step to decompose the protein in blood to produce an amino acid; A solid-liquid separation step of removing an undifferentiated protein in blood after the proteolytic step to obtain an amino acid solution; And a sterilization treatment step of sterilizing the amino acid solution obtained in the solid-liquid separation step, wherein the protein decomposition step comprises decomposing the protein into a peptide by a proteolytic enzyme and decomposing the peptide into an amino acid by a peptide decomposing enzyme And the amount of amino acid production is increased.

Wherein the proteolytic step comprises the steps of: a) a primary disaggregation step of adding proteolytic enzyme to the blood to decompose the protein in the blood into peptides; and b) a secondary disaggregation step of decomposing the peptide into amino acids by adding a peptide degrading enzyme after the primary disaggregation step .

In the primary disaggregation step, 0.02 part by weight of savinase as the protease is added to 100 parts by weight of blood, and the digestion is performed at 38 to 42 ° C for 1 to 4 hours. 0.15 parts by weight of flavorzyme is added to 100 parts by weight of blood, and the mixture is decomposed at 63 to 67 ° C for 1 to 5 hours.

In the sterilization treatment step, the amino acid solution is sterilized by heating to 88 to 92 占 폚.

As described above, the present invention can sequentially increase the amount of amino acid production by progressing the protein degradation by the protease and the peptide degradation by the peptide degrading enzyme sequentially in separate processes.

1 is a block diagram schematically illustrating a method for producing amino acids in livestock blood according to an embodiment of the present invention,
Figure 2 is a graph of amino acid content according to a comparative example,
Figures 3 and 4 are graphs of amino acid content according to the examples,
5 is a schematic view illustrating a deodorizing apparatus for deodorizing according to another embodiment of the present invention.

Hereinafter, a method for producing amino acids in livestock blood according to a preferred embodiment of the present invention will be described in detail with reference to FIG.

A method for producing amino acids in livestock blood according to an embodiment of the present invention includes a collecting step of collecting blood of livestock generated in a slaughterhouse, a pulverizing step of pulverizing the by-products contained in the blood collected in the collecting step, A protein-decomposing step of adding an enzyme to the blood to decompose the protein in the blood to produce an amino acid; a solid-liquid separation step of obtaining an amino acid solution by removing undissolved proteins in the blood after the protein decomposition step; And a sterilization treatment step of sterilizing the solution. Let's look at each step.

1. Collection stage

Collect the blood of livestock from the slaughterhouse. The slaughter of livestock is carried out at slaughter facilities such as slaughterhouses. Cattle can be cattle, pigs, chickens, and ducks. Blood generated during slaughter of livestock can be prevented from contamination by transporting the blood to the storage tank through a pipeline installed at the slaughterhouse.

Blood is stored in a storage tank kept at 4 ° C or lower because corruption proceeds after collection.

2. Grinding step

Blood collected during the slaughter of livestock in slaughterhouses contains by-products such as blood vessels and tendons. Therefore, before the protein is decomposed, the by-products are finely pulverized and, at the same time, subjected to a pulverizing step to obtain uniform properties. The decomposition of the protein can be facilitated through such a grinding process.

Conventional pulverizers can be used to pulverize the by-products contained in the blood. A high-speed liquid pulverizer can be used as a conventional pulverizer. In addition, a pulverizer can be installed inside the above-mentioned pipeline to simultaneously perform pulverization while passing blood through a pipeline.

3. Proteolysis step

After completion of the grinding step, an enzyme is added to the blood to decompose the protein in the blood to produce an amino acid.

After the pulverized blood is put into the reactor, the protein in the blood is decomposed into amino acids in the reactor using the enzyme. An agitating impeller is installed inside the reactor to decompose the blood while stirring. The blood of livestock is rich in various proteins. Particularly, the plasma part contains protein components such as albumin and globulin, and the hemocyte part contains a large amount of protein component called hemoglobin.

The present invention uses a protease that hydrolyzes a protein to a peptide as an enzyme and a peptide degradase that hydrolyzes the peptide to an amino acid. An enzyme that decomposes a protein into peptides, such as pepsin or trypsin, can be used as a protease. And sabinase of NOVOZYMES commercialized as a protease can be used.

An enzyme that decomposes a peptide into an amino acid, such as a peptidase, may be used as a peptide degrading enzyme. And flavorzyme of NOVOZYMES commercialized with such a peptide degrading enzyme can be used.

In the present invention, the protease and the peptide decomposing enzyme are sequentially processed in separate steps to improve the yield of amino acid production.

Specifically, the proteolytic step comprises the steps of: a) a primary disaggregation step of adding proteolytic enzyme to the blood to decompose proteins in the blood into peptides; and b) a step of dissolving the peptide into amino acids by adding a peptide degrading enzyme after the primary disaggregation step It is made up of the stages of distillation.

A proteolytic enzyme is added to the reactor to perform primary digestion, and then the peptide digestion enzyme is added to the reactor, followed by secondary digestion.

In the primary disaggregation step, 0.02 parts by weight of protease is added to 100 parts by weight of blood, and decomposed at 38 to 42 캜 for 1 to 4 hours. In the second disintegration step, 0.15 part by weight of peptide decomposing enzyme is added to 100 parts by weight of blood, and the digestion is carried out at 63 to 67 ° C for 1 to 5 hours.

As described above, unlike the conventional method of decomposing a protein into an amino acid through a single process by mixing a protease and a peptide degrading enzyme, the protease and the peptide decomposing enzyme are sequentially processed by separate processes, Can be increased.

4. Solid-liquid separation step

After the protein is decomposed, solid-liquid separation is performed in order to remove undissolved proteins in the blood in the reactor.

For solid-liquid separation, a conventional centrifugal separator or a filter press may be used. The solid residue is separated from the amino acid solution by solid-liquid separation.

5. Sterilization treatment step

The amino acid solution obtained through solid-liquid separation is sterilized.

The sterilization can be performed by sterilizing the amino acid solution by heating to 88 to 92 占 폚. The sterilized amino acid solution can be stored in a sealed container. The amino acid solution can be diluted 1000 times or more to be useful for fertilizer and feed.

As another example of the present invention, a deodorization step may be further performed after the solid-liquid separation step.

5, the amino acid solution obtained in the solid-liquid separation step is introduced into the deodorization tank 10, and then the amino acid solution is stirred using the stirring impeller 25 provided in the deodorization tank 10. The stirring impeller 25 is rotated by a motor 20 installed on the deodorizing tank 10. [ Then, with the stirring of the amino acid solution, the air is aerated into the amino acid solution to remove the odor component. To this end, an air diffuser 30 is installed below the deodorization tank 10, and the air diffuser 30 is connected to the air compressor 35 to supply air. By operating the air compressor (35), air is aerated into the amino acid solution and the odor component in the ammonia solution is deaerated. The odor component can be discharged to the outside through an exhaust pipe provided on the deodorization tank (10).

After the deodorization step, the amino acid solution is sterilized.

Hereinafter, the present invention will be described by way of examples. However, the following examples are intended to illustrate the present invention in detail, and the scope of the present invention is not limited to the following examples.

(Example)

The pig blood collected from the slaughterhouse was pulverized by a pulverizer, 0.02 part by weight of sabinase (Novozymes, Denmark) was added to 100 parts by weight of blood, and the protein was first degraded with the peptide at 40 ± 2 ° C with stirring. 0.15 parts by weight of flavorzyme (Novozymes, Denmark) was added to 100 parts by weight of blood, and the peptide was secondarily degraded with an amino acid while stirring at 65 ± 2 ° C.

(Comparative Example)

The pig blood used in the examples was digested with the same method as the conventional technique (Patent Registration No. 10-135614).

Namely, 0.1 part by weight of an enzyme obtained by mixing Protease (Novozymes, Denmark), a protease, and Flavois 500MG (Novozymes, Denmark), a peptide degrading enzyme, in the same weight ratio was added to 100 parts by weight of blood, The mixture was stirred at 50 ± 2 ° C and decomposed into amino acids.

<Blood analysis>

The blood used in the examples was analyzed by the Kjeldahl flask decomposition method and the contents of water, protein and amino acid in the blood are shown in Table 1 below.

moisture protein amino acid 87.8 (% / 100g) 12.23 (% / 100g) 183 (mg / 100g)

The blood before the digestion showed 87.8% moisture, 12.23% protein and 183 mg amino acid.

<Amino acid content>

In order to examine the proteolytic effect, the amino acid content (mg / 100 g) with time was measured in the comparative example, and the results are shown in Table 2 and FIG.

division After 1 hour After 2 hours After 3 hours After 4 hours After 5 hours After 6 hours After 7 hours Comparative Example 213 247 253 269 290 314 306

Referring to Table 2, when the protease and the peptide degrading enzyme were mixed and applied simultaneously, the amino acid content was 213 mg / 100 g after 1 hour of reaction and 290 mg / 100 g after 5 hours.

Meanwhile, in the examples, the amino acid content with time was measured.

First, the results of the first decomposition by sabinase enzyme are shown in Table 3 and FIG. 3, respectively.

division 0.5 hours  1 hours 1.5 hours 2 hours 2.5 hours 3 hours 3.5 hours 4 hours 5 hours 1st disintegration 213 235 242 248 254 266 275 288 280

Referring to Table 3, it was revealed that the protein was degraded into peptides and partially decomposed into amino acids at the first decomposition by the enzyme sabinase. As a result, the content of amino acid was 235 mg / 100 g after 1 hour of the first decomposition with the enzyme sabinase, and 280 mg / 100 g after 5 hours. It was found that there was no significant difference when the two enzymes were mixed and applied at the same time in comparison with the comparative example.

On the other hand, the amino acid content was lower than that after 4 hours of reaction after 5 hours of reaction at the first decomposition by sabinase enzyme. Therefore, it was confirmed that the reaction time in the first decomposition was suitable from 1 to 4 hours.

The results of the secondary degradation by the flavorzyme enzyme are shown in Table 4 and FIG. 4, respectively.

division 1 hours 2 hours 3 hours 4 hours 5 hours 6 hours Secondary disintegration 455 967 1650 3490 4329 3923

As a result, the content of amino acids was 455 mg / 100 g after 1 hour of reaction, and 1650 mg / 100 g after 3 hours and 4329 mg after 5 hours from the first decomposition by enzymatic digestion with flavinzyme / 100g, the amino acid content was significantly increased over time. After 6 hours of reaction, amino acid content was lower than after 5 hours of reaction. Therefore, it was confirmed that the reaction time in the secondary decomposition was 1 to 5 hours.

From the above experimental results, it was shown that the amino acid production can be greatly increased by decomposing the protein at a different reaction temperature and reaction time suitable for each enzyme.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation. Accordingly, the true scope of protection of the present invention should be determined only by the appended claims.

Claims (5)

A collecting step of collecting blood of livestock in a slaughterhouse;
A pulverizing step of pulverizing the by-products contained in the blood collected in the collecting step;
A protein decomposition step of adding an enzyme to blood after completion of the pulverization step to decompose the protein in blood to produce an amino acid;
A solid-liquid separation step of removing an undifferentiated protein in blood after the proteolytic step to obtain an amino acid solution;
And a sterilizing treatment step of sterilizing the amino acid solution obtained in the solid-liquid separation step,
Wherein the proteolytic step comprises separately performing a process of decomposing a protein into a peptide by a protease and a process of decomposing the peptide into an amino acid by a peptide degrading enzyme to increase the amount of amino acid produced, / RTI &gt; The method according to claim 1, wherein the proteolytic step comprises the steps of: a) a primary disaggregation step of adding proteolytic enzyme to the blood to decompose the protein in the blood into peptides; and b) adding the peptide degrading enzyme And a second disintegration step of decomposing the amino acid in the animal blood. [3] The method according to claim 2, wherein the first decomposing step comprises the step of adding 0.02 part by weight of savinase as the protease to 100 parts by weight of blood, decomposing at 38 to 42 [deg.] C for 1 to 4 hours,
Wherein the second digestion step comprises adding 0.15 parts by weight of flavorzyme as a peptide degrading enzyme to 100 parts by weight of blood and digesting the mixture at 63 to 67 ° C for 1 to 5 hours to produce an amino acid How to. The method according to claim 1, wherein the sterilization treatment step comprises sterilizing the amino acid solution by heating to 88 to 92 占 폚. The method according to claim 1, further comprising a deodorization step of deodorizing the amino acid solution by aerating the air into the amino acid solution after the solid-liquid separation step.

Images