KR20180008179A - Natural liposome comprising red yeast rice, process for the preparation thereof, and food or pharmaceutical composition comprising the same - Google Patents

Abstract

The present invention relates to a liposome-forming material comprising at least one natural emulsifier, a natural-derived solvent, a natural coating agent and a natural preservative; and a natural liposome comprising red yeast rice, which comprises a red yeast rice extract as a capturing substance, and which is formed by mixing the natural emulsifier, the natural-derived solvent, and the red yeast rice extract through ultrasonication. The present invention also relates to a method for manufacturing the natural liposome comprising red yeast rice, and to a food or pharmaceutical composition comprising the same. It is an object of the present invention to provide the natural liposome comprising the red yeast rice, which is harmless to a human body and can be easily added to a living body to exhibit excellent blood circulation improving effect, a manufacturing method thereof, and the food or pharmaceutical composition comprising the same.

Description

TECHNICAL FIELD The present invention relates to a natural liposome containing a red yeast, a method for producing the same, and a food or a pharmaceutical composition containing the same. BACKGROUND OF THE INVENTION 1. Field of the Invention [0002]

The present invention relates to a natural liposome containing red yeast, a method for producing the same, and a food or pharmaceutical composition containing the same.

Red yeast rice is fermented by inoculating rice with Monascus sp., And contains various beneficial metabolites produced during the fermentation process. In particular, monacolin K, one of the functional ingredients contained in red ginseng, is known to inhibit cholesterol biosynthesis in the liver, to lower the low density (LDL) cholesterol level and to increase the high density (HDL) cholesterol level . In addition, the red yeast contains gamma aminobutyric acid, which inhibits accumulation of cholesterol and triglycerides in blood vessels, thereby allowing blood to flow smoothly and reducing blood pressure.

In order to utilize the various effects of Hongkuk in this way, the development of products such as foods, health supplements, and medicines using Hongkuk is actively under way. Korean Patent Laid-Open Publication No. 2014-0037294 discloses a gum chip obtained by inoculating and fermenting Ganoderma sp. Gum. Korean Patent Laid-Open Publication No. 2013-0029890 discloses a composition containing monasin extracted from Ganoderma ganoderma, Lt; / RTI > When these products are consumed, the excellent effect by the red yeast can be expected, but the absorption rate of the red yeast is different for each individual, and the effect of the red yeast may also be different. Therefore, it is required to develop a technique for enhancing the absorption of red yeast into the body so that the same effect can be exhibited even when the red yeast-containing product is consumed. At this time, as a method for increasing the absorption of red yeast into the body, a biological drug carrier such as liposome can be used.

Liposomes are artificially produced vesicles composed of lipid bilayers and have a structure similar to a vital cell membrane. The liposomes are biocompatible and composed of a phospholipid, which is a major component of a vital cell membrane, and exist in the form of capsules in which a hydrophilic and hydrophobic substance in an aqueous solution is surrounded by a lipid bilayer of liposome. Such liposomes are capable of storing desired useful substances (drugs, nutrients, etc.) in the bilayer structure, and are capable of transporting unstable and difficult-to-handle substances into cells. However, in spite of these advantages, liposomes have problems in that they are unstable in formulation, have a very low collection efficiency, and contain synthetic materials such as solvents and preservatives used in the production of liposomes and are harmful to human body. In addition, the cholesterol used as a raw material of the liposome has a problem that it forms a liposome lipid membrane firmly and hinders the release of the trapping material and lowers the cell membrane permeability of the trapping material. It is still necessary to develop a technique for solving the problems of the liposome and increasing the absorption rate of a useful substance, that is, a stable state of the red yeast into the body.

In addition, the existing extract containing red ginseng is known, but the substance containing the red liposome containing red ginseng and the natural liposome containing red ginseng has not been known.

Korean Patent Publication No. 2014-0037294 Korea Patent Publication No. 2013-0029890

Disclosure of Invention Technical Problem [6] The present invention provides a natural red lip gum containing red yeast which is harmless to human body and can be easily added to a living body and exhibits excellent blood circulation improving effect, a process for producing the same, and a food or pharmaceutical composition containing the same.

One example of the present invention is a liposome comprising a natural emulsifier, a natural-derived solvent, a natural coating agent and a natural preservative; And a red yeast extract as a capturing substance, and the natural emulsifier, the natural-derived solvent, and the red yeast extract are ultrasonicated and mixed to provide a red yeast-containing natural liposome.

Another example of the present invention is a process for producing a microcapsule comprising (a) a first step of mixing at least one natural emulsifier with a natural-derived solvent and ultrasonication at 35 to 80 캜; (b) adding a red gingko extract to the mixture of the first step and ultrasonically treating the mixture at 35 to 80 캜 for mixing; (c) a third step of adding a natural coating agent to the mixture of the second step and ultrasonication at 35 to 80 캜; And (d) a fourth step of adding a natural-derived solvent and a natural preservative to the mixture of the third step and sonicating at 35 to 80 ° C.

Another example of the present invention provides a food or a pharmaceutical composition comprising the red rice bran-containing natural liposome.

The red lipped natural liposome according to the present invention can increase the absorption rate of red ginseng extract, which is a capturing substance, and improve the effect caused by red ginseng. Specifically, the food or pharmaceutical composition containing the red lupus-containing natural liposome can absorb cholesterol biosynthesis, control cholesterol and triglyceride levels in blood, and improve blood circulation by absorbing functional ingredients of red yeast into the body.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view of a method for producing a red lentil-containing natural liposome according to an embodiment of the present invention.
2 is a graph showing the result of particle size analysis according to Experimental Example 1 (1).
Fig. 3 is a graph showing the results of skin absorption rate test according to (2) of Experimental Example 1. Fig.
FIG. 4 is a graph showing the results of measurement of total cholesterol secretion amount according to Experimental Example 2. FIG.
5 is a graph showing the results of measurement of blood triglyceride content according to Experimental Example 3 (1).
6 is a graph showing the results of measurement of total cholesterol content in blood according to Experimental Example 3 (1).
FIG. 7 is a graph of HDL cholesterol content measured in blood according to Experimental Example 3 (1). FIG.
8 is a graph showing the results of measurement of blood LDL cholesterol content according to Experimental Example 3 (1).
9 is a graph showing the results of measurement of AST content in blood according to (1) of Experimental Example 3. FIG.
10 is a graph showing ALT content in blood according to Experimental Example 3 (1).
11 shows the results of liver tissue observation according to (2) of Experimental Example 3. Fig.
12 shows the results of liver tissue observation according to (2) of Experimental Example 3. Fig.

Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings, wherein the natural liposome containing a red yeast according to the present invention, the method for producing the same, and the food or pharmaceutical composition containing the same. It should be understood, however, that such description is illustrative only and is not intended to limit the scope of the present invention.

< Hongkuk  Containing natural Liposome >

Liposomes according to an example of the present invention may comprise a liposome constituent comprising at least one natural emulsifier, a natural derived solvent, a natural coating and a natural preservative; And a red yeast extract as a collecting material, wherein the natural emulsifier, the natural-derived solvent and the red yeast extract are mixed by ultrasonic treatment to provide a red yeast-containing natural liposome. The natural liposome is distinguished from the conventional synthetic liposome in that it does not contain a synthetic component.

1. Liposome constituent material

The liposomes of the present invention are natural liposomes including natural emulsifiers, natural solvents, natural coatings and natural preservatives. Natural liposomes made of such natural materials are harmless because they are not toxic to the human body and can be ingested. In addition, the liposome can form a stable phospholipid bilayer, thereby effectively delivering the capturing material to the cell.

[Natural emulsifier]

Natural emulsifiers are emulsifiers derived from natural materials and constitute a lipid bilayer. The natural emulsifiers include phospholipids and fatty acids. The phospholipid contained in the natural emulsifier is a natural phospholipid and the fatty acid is a natural fatty acid derived from natural.

Phospholipids are contained in large amounts in egg yolk, soybean, etc., and they can be obtained by extraction, separation and purification from these. Examples of the phospholipid include phosphatidyl choline (lecithin), lysophosphatidyl choline, phosphatidyl ethanolamine, phosphatidic acid, phosphatidyl serine, phosphatidyl glycerol, , Phosphatidyl inositol, and their hydrogenation products (for example, hydrogenated phosphatidyl choline - derived from soy) can be used. Phosphatidyl choline, lysophosphatidyl choline, or hydrogene phosphatidyl choline, which are excellent in oxidation stability, can be used.

The fatty acid is contained in many seeds such as walnuts, pecans, almonds, pine nuts, olives, corn, flax, cotton, rapeseed flowers and evening primrose. The fatty acid may be at least one fatty acid selected from the group consisting of palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid .

The natural emulsifier may include 70 to 90% by weight of a natural phospholipid and 10 to 30% by weight of a natural fatty acid based on 100% by weight of a total amount of a natural phospholipid and a natural fatty acid. The natural emulsifier may contain remaining amount of other solvent. As the solvent, it is preferable to use a naturally derived solvent, and it is more preferable to use distilled water.

The natural emulsifier may be used by mixing two or more kinds thereof. For example, when two natural emulsifiers are mixed and used, for example, the first natural emulsifier may be selected from the group consisting of phosphatidyl choline, lysophosphatidyl choline, phosphatidyl ethanolamine, palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid (derived from soybean), and the second natural emulsifier may be hydrogeneated phosphatidyl choline phosphatidyl choline) can be used. In addition, when three natural emulsifiers are mixed and used, for example, in addition to the first natural emulsifier and the second natural emulsifier, cetearyl olivate and sorbitan olivate sorbitan olivate) can be used.

In addition, the natural emulsifier may further include a natural fatty acid. As the natural fatty acid, evening primrose oil may be used. The content of the natural fatty acid may be 0.1 to 5% by weight based on the weight of the whole natural emulsifier.

The content of the natural emulsifier is preferably 0.1 to 10% by weight based on the weight of the whole natural liposome.

[Natural solvent]

The natural-derived solvent allows the lipid bilayer of the natural emulsifier to be in the form of a capsule surrounding the capture material.

As the solvent, at least one solvent selected from the group consisting of natural solvents, for example, distilled water, natural-derived glycerin, natural ethanol, natural propanediol, natural glycerin and fermented alcohol can be used. Preferably, distilled water, natural-derived glycerin, and natural ethanol can be used, and more preferably, distilled water can be used.

The content of the solvent is preferably a residual amount excluding a natural emulsifier, a natural coating agent, a natural preservative, and a capturing agent based on the weight of the whole red lug containing natural liposome.

[Natural preservative]

Natural preservatives are substances that maintain the stability of the final red yeast-containing natural liposome by preventing decomposition of liposome constituents (natural emulsifiers, natural solvents, natural coatings) and trapping substances present in liposomes.

The natural preservative may be any natural preservative that can be added to foods or medicines. For example, natural extracts can be used. Citrus extract, citron extract, eucalyptus extract, ginseng extract, clove extract and grapefruit extract may be used. Citrus extract and grapefruit extract may be preferably used. The preservative is a natural preservative and is usually used in food preservatives such as dehydroacetic acid, sorbic acid, benzoic acid, methyl p-hydroxybenzoate, propanoic acid ) And salts thereof, and is harmless to the human body because it is not toxic.

Natural preservatives that can be used as cosmetic preservatives when used as cosmetics can be used without limitation. For example, an extract of Chrysanthemum morifolium, a flower of Chrysanthemum morifolium, and an extract of Asnuria may be used.

The content of such a natural preservative is preferably 0.001 to 3% by weight, more preferably 0.01 to 0.3% by weight, based on the total weight of the natural liposome.

2. Collecting material

The capturing material according to the present invention is a substance trapped in the lipid bilayer of the liposome containing the natural emulsifier, the natural solvent, the natural coating agent and the natural preservative. As the capturing material, red yeast can be used.

The red ginseng contains beneficial metabolites produced during the fermentation process by the ginseng, and especially the effects of monacocholine K on blood lipid composition improvement and blood circulation are known. The red yeast is processed into powder or liquid form for easy ingestion, and is used as a liposome capturing material to increase the absorption rate in the body. Preferably, the red rice cake powder can be used as a capturing material. At this time, the molecule size can be reduced by ultrasonication in order to increase the absorption rate in the body while preventing the functional ingredient of the red yeast extract from being destroyed. These red yeast extracts can be used by being sufficiently dissolved in butylene glycol, propanediol, glycerin, and fermented alcoholic beverages derived from natural materials so that they can be easily mixed with the liposome constituting materials.

The content of the red yeast extract is preferably 0.1 to 30% by weight based on the total weight of the red yeast-containing natural liposome.

3. Other

A coating agent may be further included as a component for coating other capture materials. As the coating agent, a natural coating agent is preferably used. Examples of the coating agent include dietary fiber, starch, polysaccharide and the like, and examples thereof include maltodextrin, tapioca, guar gum and corn starch. It is preferable to use maltodextrin in view of its high stability so that it is well absorbed.

In addition, an absorption aid may be further included to assist the liposome surrounding the capture material to be well absorbed in the body. As the absorption aid, plant extract powder containing a large amount of caffeine may be used. For example, guarana extract powder and the like may be used. The natural liposome containing red ginseng root containing natural absorption aids has an increased intracellular absorption rate of the trapping substance in the body, and can exert excellent effects by the red ginseng root.

The content of the natural absorption adjuvant is preferably 0.001 to 3% by weight based on the total weight of the red lipid-containing natural liposome.

As described above, the red lentil containing the red yeast of the present invention is a capsule form surrounded by the lipid bilayer and capable of easily transferring the red yeast extract into cells. When the natural liposome containing the red yeast is transferred to the cells, the lipid bilayer and the cell membrane are easily combined with each other, thereby increasing the cell uptake rate of the red yeast extract. Thus, inhibition of cholesterol synthesis by the red yeast, control of cholesterol and triglyceride levels in the blood, Effect can be expected.

< Hongkuk  Containing natural Liposomal  Manufacturing method>

The present invention provides a method for preparing a red lentil containing natural liposome by treating ultrasound to uniformly mix the lipid and the liposome constituent. However, the present invention is not limited to such a production method, and the steps of each process may be modified or optionally mixed as necessary.

As shown in FIG. 1, a preferred method for preparing a red lipped natural liposome according to an embodiment of the present invention includes: (a) mixing a natural emulsifier with a natural-derived solvent, and performing ultrasonic treatment at 35 to 80 ° C ; (b) adding a red gingko extract to the mixture of the first step and ultrasonically treating the mixture at 35 to 80 캜 for mixing; (c) a third step of adding a natural coating agent to the mixture of the second step and ultrasonication at 35 to 80 캜; And (d) adding a natural-derived solvent and a natural preservative to the mixture of the third step and sonicating at 35 to 80 ° C. After the fourth step, it may further include a fifth step of repeatedly performing ultrasonic processing while gradually lowering the temperature.

Hereinafter, the manufacturing method will be described separately for each process step as follows.

(a) Step 1

This step is a step of mixing one or more natural emulsifiers with a natural-derived solvent and ultrasonication at 35 to 80 캜 for mixing.

As the natural solvent, distilled water may be used or butylene glycol, propanediol, glycerin, fermented alcohol and the like derived from a natural substance may be used.

As the natural emulsifier, at least one natural emulsifier containing a phospholipid and a fatty acid derived from a natural substance may be used.

The ultrasonic treatment is a work for homogenizing a mixture of a natural solvent and a natural emulsifier, and an ultrasonic liquid processor may be used.

The natural-derived solvent and the natural emulsifier are mixed and then heated to 35 to 80 ° C, preferably 50 to 75 ° C, more preferably 60 to 70 ° C, and then ultrasonicated to obtain the phospholipid of the natural emulsifier And fatty acids can be homogenized. In this temperature range, the emulsifier is a temperature range favorable for stably forming the liposome.

(b) Step 2

In this step, the red yeast extract is added to the mixture of the first step, and then the mixture is ultrasonicated at 35 to 80 ° C to mix.

The red yeast extract may be obtained by pulverizing or extracting red yeast in a liquid phase and drying it under conditions of temperature, pressure and humidity that do not destroy functional ingredients. At this time, a method of crushing red rice can be used without limitation, a conventional crushing method known in the art. In addition, the method for extracting the red yeast into a liquid phase can be carried out by any conventional extraction method known in the art without limitation. For example, extracts in liquid or powder form can be obtained using extraction methods such as cold extraction, ultrasonic extraction, reflux cooling extraction, and hot water extraction. The red ginseng extract may be used in a form dissolved in butylene glycol, propanediol, glycerin, fermented alcohol, etc., derived from a natural substance so as to be uniformly mixed with the mixture of the first stage. When the red yeast extract is dissolved in a fermented mushroom, the mixture ratio of the red yeast extract and the fermented mash is 1: 1, preferably 0.3: 1, based on the weight ratio.

Through two steps, each component can be mixed homogeneously and a liposome with a small particle size can be formed. The ultrasonic treatment can be carried out, for example, after adding the red yeast extract to the mixture of the first step and mixing the mixture by heating to 35 to 80 캜. However, if the mixture of the first stage maintains the above-mentioned temperature range, ultrasonic treatment can be performed without a separate heating process.

(c) Step 3

This step is a step of adding a natural coating agent to the mixture of the second step and ultrasonication at 35 to 80 캜. The ultrasonic treatment can be carried out, for example, after adding a natural coating agent to the mixture of the second step and mixing by heating at 35 to 80 캜. However, if the mixture of the second stage maintains the temperature range, the ultrasonic treatment can be performed without a separate heating process.

The natural coating agent helps absorption of the red lupine-containing natural liposome to be finally produced.

(d) Step 4

This step is a step of adding a natural-derived solvent and a natural preservative to the mixture of the third step and sonicating at 35 to 80 ° C. The natural-derived solvent and the natural preservative may be preheated to the above temperature before ultrasound can be performed.

As the natural solvent, the same solvent as that used in the first step may be used.

The natural preservatives prevent the decay of liposome constituent materials (natural emulsifiers, natural solvents, natural coating agents) and the trapping substances present in the liposomes and thus maintain the stability of the red lipids containing red yeast. In addition, the natural preservative is derived from a natural substance and is not toxic to the human body and does not damage the natural liposome containing red yeast.

(b) the second step, (c) the third step, and (d) the fourth step, the ultrasonic treatment is carried out at 35 to 80 ° C, preferably at 60 to 70 ° C.

(e) Step 5

This step is repeatedly performing ultrasonic treatment while gradually lowering the temperature after the fourth step.

The red lip gloss containing red yeast produced by the above method is subjected to ultrasonic treatment at a temperature of 35 to 70 DEG C for a time of 1 to 10 minutes, preferably 3 to 8 minutes, while gradually lowering the temperature, Thereby producing a smaller and more uniform red lipid-containing natural liposome.

The ultrasonic treatment can be repeatedly carried out to produce a red lipid-containing natural liposome having a desired particle size, preferably 1 to 3 times.

As described above, the process for producing a natural liposome containing red yeast according to the present invention can form a natural liposome containing a red yeast extract which is harmless to the human body and has a small particle size, thereby increasing the absorption rate of red yeast to cells .

< Hongkuk  Containing natural Liposomes  Containing food or pharmaceutical composition &

One example of the present invention provides a food composition or a pharmaceutical composition containing the red luged natural liposome as an active ingredient or containing the red luged natural liposome as an active ingredient.

The natural liposome containing red yeast according to an embodiment of the present invention binds with the cell membrane to increase the cell uptake rate of the red yeast extract so that the functional ingredient of the red yeast can effectively function in the human body. When the natural liposome containing the red rice bran is ingested, it can be used as a food or a pharmaceutical composition containing the red lanugosium-containing natural liposome as an effective ingredient, because the red rice bran absorbed in the intestines may be effective.

The food or pharmaceutical composition may contain, as an active ingredient, a natural liposome containing red ginseng and a natural liposome containing red ginseng. The natural liposome containing the red ginseng is a liposome constituting material containing at least one natural emulsifier, a natural solvent, a natural coating agent, a natural preservative, and a red ginseng extract as a trapping material. The red ginseng extract is used by ultrasonication to reduce the particle size. The red ginseng extract, the natural emulsifier, and the natural solvent are ultrasonicated to form a uniformly mixed natural liposome containing red ginseng. Red ginseng is known to have antioxidant, antiinflammation, improvement of lipid composition in blood, and improvement of blood circulation. Therefore, the effect of red ginseng can be expected when the natural liposome containing the red ginseng is ingested. Therefore, in the composition containing the red lanthanum-containing natural liposome and the natural liposome containing red ginseng, the red ginseng effect as well as the red ginseng effect can be exhibited, and the synergy effect of these effects can be expected. In order to further enhance the effect of such a composition, one or more extracts selected from the group consisting of red ginseng extract, red ginseng extract, garlic extract, onion extract, cinnamon extract, 칡 extract, white tea extract and soybean extract may be further contained.

Such a food or pharmaceutical composition may contain 1 to 40% by weight of the red yeast-containing natural liposome, 10 to 50% by weight of the red ginseng-containing natural liposome and 5 to 50% by weight of the extract, based on the total weight.

The food composition of the present invention can be used without limitation in the kind of food, and can be used as an additive to general food or as a health supplement. Preferably a health supplement for blood circulation improvement. Since the food composition is composed of a natural food and its fermentation product having little toxicity and side effects, it can be safely used for prolonged use for preventive purpose. Such food compositions may include food additives known in the art and include, for example, sugars, amino acids, vitamins, minerals (inorganic salts), sweeteners, flavors, flavors, colorants, thickeners, Preservatives, and the like.

The pharmaceutical composition of the present invention can be used in the form of a general pharmaceutical preparation which can be administered orally or parenterally. Preferably as tablets, hard or soft capsules, solutions, suspensions, and the like. Such pharmaceutical compositions may comprise conventional pharmaceutically acceptable carriers, including, for example, excipients, binders, disintegrants, lubricants, solubilizers, suspending agents, preservatives, extenders and the like in the case of preparations for oral administration .

The food or pharmaceutical composition containing the natural liposome containing red kidney or the natural liposome containing red ginseng as an active ingredient is effective for improving lipid and blood circulation by controlling the levels of cholesterol and triglyceride in the blood, Or to alleviate ongoing arteriosclerotic symptoms.

Hereinafter, the present invention will be described concretely with reference to Examples. However, the following Examples are intended to illustrate one embodiment of the present invention, but the scope of the present invention is not limited by the following Examples.

[ Preparation Example  One] Hongkuk  Containing natural Liposomal  Produce

The natural liposomes containing red yeast were prepared according to the composition shown in Table 1 below.

Composition Content (% by weight) Natural emulsifier Distilled water 30.00 Lipoid P75
Or DS-Soya PC60 3.00 Soy lecithin 2.00 Capture material Red rice powder 3.00 Fermented alcohol 10.00 Natural coating agent Guarana extract 0.50 Natural preservative Grapefruit extract 0.03 menstruum Distilled water 51.47

First, distilled water warmed to 65 占 폚 was added with Lipoid P75 (67% by weight of phosphatidylcholine, 8% by weight of lysophosphatidylcholine, 8% by weight of phosphatidylethanolamine, 3% by weight of palmitic acid, (100% soybean lecithin containing 60.5% of phosphatidylcholine and 1.9% lysophosphatidylcholine) was put into an ultrasonic wave crusher (containing 1% by weight of acid, 3% by weight of oleic acid, 7% by weight of linoleic acid and 3% Ultrasonic Liquid Processor) and homogenized for 2 minutes.

The collected material was prepared by mixing red yeast powder (GUNZE LIMITED, Japan, GUNZE RED YEAST RICE 3P-D20) and a fermented alcohol at a weight ratio of 0.3: 1, then the collected material was slowly added to the homogenized natural emulsifier, And homogenized for 2 minutes in total for 3 seconds.

When the natural emulsifier and the capturing material were completely homogenized, the guarana extract heated to 65 ° C was added, and the mixture was further disintegrated for 10 seconds and then stopped for 3 seconds and homogenized for 2 minutes in total. The grapefruit extract and the remaining distilled water were added to the homogenized solution, disrupted for 10 seconds, stopped for 3 seconds, and homogenized for 2 minutes in total. Thereafter, the mixture was crushed for 10 seconds and stopped for 3 seconds to homogenize the whole for 2 minutes to prepare a red lupine-containing natural liposome.

[ Preparation Example  2] Red ginseng containing natural Liposomal  Produce

The natural liposome containing red ginseng was prepared according to the composition shown in Table 2 below.

Composition Content (% by weight) Natural emulsifier Lipoid P75
Or DS-Soya PC60 2.500 Capture material Red ginseng extract 5.000 Natural coating agent Maltodextrin 1,000 Natural preservative Grapefruit extract 0.015 Natural solvent Distilled water 91.485

First, a natural emulsifier (Lipoid s75, Lipoid GMBH) was mixed with distilled water, heated to 65 ° C and crushed using an ultrasonic liquid processor. The red ginseng extract (product containing 24.4 mg / g (at least 2.5 to 15 mg / g or more) of the sum of Bioland, red ginseng concentrate powder A and ginsenoside Rb1, Rg1 and Rg3) and distilled water were mixed at a weight ratio of 1: When the natural emulsifier was homogenized, the prepared collecting material was added, followed by ultrasonic disruption and mixing. When the natural emulsifier and the capturing material were completely homogenized, maltodextrin was added and the homogenization was performed by ultrasonic disintegration. Then, the remaining distilled water heated to 50 &lt; 0 &gt; C and grapefruit extract were added and ultrasonically disrupted to form liposome type particles. The temperature was gradually lowered, and finally ultrasonic disruption was repeated three times for 2 minutes and 30 seconds to homogenize, thereby preparing a natural liposome containing red ginseng.

[ Example  One] Hongkuk  Containing natural Liposome

The red lipped natural red liposome prepared in Preparation Example 1 was used.

[ Example  2] Hongkuk  Containing natural Liposomes  Natural red ginseng Liposomal  mixture

According to the composition shown in Table 3 below, a mixture was prepared using the red lip gloss containing red lips prepared in Preparation Example 1, the red lip rouge used in Preparation Example 1 and the red lip gloss containing red ginseng prepared in Preparation Example 2 above.

Raw material Content (% by weight) Red-noodle-containing natural liposome 2 Natural liposome containing red ginseng 30 Red rice powder 12 Garlic extract 15 sugars 25 Soy lecithin 15

[ Comparative Example  One] Hongkuk  powder

The red rice powder used in Preparation Example 1 was used.

[ Comparative Example  2] Red ginseng containing natural Liposome

The red liposome containing red ginseng prepared in Preparation Example 2 was used.

[ Comparative Example  3] Red ginseng extract

The red ginseng extract used in Preparation Example 2 was used.

[Comparative Example 4] Gamma linolenic acid

Gamma linolenic acid (Sanmark, China) was used.

[Comparative Example 5] Atorvastatin

Atorvastatin (SIGMA, USA, Atorvastatin Calcium, # PHR1422) was used.

[ Experimental Example  1] Characteristic analysis

(1) particle size analysis

The red lipstick of Example 1, the red lipstick of Comparative Example 1, the natural liposome containing red ginseng of Comparative Example 2 and the red ginseng extract of Comparative Example 3 were obtained using a laser particle size analyzer (Electrophoretic Light Scattering Spectrophotometer ELS-8000, Otsuka) Size, and particle size distribution. The results are shown in Fig.

As shown in Fig. 2, the particle size of the red lip gloss of red lentil (Example 1) was smaller than that of red lye powder (Comparative Example 1), and the natural liposome containing red ginseng (Comparative Example 2) 3).

(2) skin absorption rate experiment

The skin absorption rate was confirmed using a Franz diffusion cell (Permegear, V3A-02, USA) equipped with pig skin.

First, the red pigment-containing natural liposome of Example 1, the red liquor powder of Comparative Example 1, the natural liposome containing red ginseng of Comparative Example 2, and the red ginseng extract of Comparative Example 3 were each treated with 1 ml of each of the pig skin at 37 ° C. The absorbance was measured by taking a sample which was permeated through pig skin according to time and released, and the result is shown in Fig.

As shown in Fig. 3, the natural liposome containing red yeast (Example 1) showed higher absorption rate than the red yeast powder (Comparative Example 1), and the natural liposome containing red ginseng (Comparative Example 2) ) Than those of the others.

[ Experimental Example  2] Experiments using hepatocytes

Total cholesterol secretion was measured using human hepatic epithelial cell HepG2. Atorvastatin and gamma linolenic acid are substances known to reduce total cholesterol secretion.

First, HepG2 was added to 96 wells at 1 × 10 ⁵ cells / well using DMEM medium (glucose 1 mg / ml, 10% FBS, 100 U / ml penicillin, 100 U / ml streptomycin) After being dispensed, the cells were cultured in an incubator (condition: 37 ° C, 5% CO ₂ ) for 24 hours. As the control group, those treated with the sample were used. As the treatment group, the red lipid-containing natural liposome of Example 1 (prepared at a concentration of 5 mg / mL and treated with 0.06%), the red lipid-containing natural liposome of Example 2, Liposomes, red yeast powder of Comparative Example 1, natural liposome containing red ginseng of Comparative Example 2, red ginseng extract of Comparative Example 3, gamma linolenic acid of Comparative Example 4, and atorvastatin of Comparative Example 5 were treated at a concentration of 25 μg / mL After culturing in an incubator for 24 hours, the medium was recovered. The absorbance of total cholesterol in the recovered medium was measured at 500 nm with a spectrophotometer (TECAN, infinite M200pro, Austria), and the results are shown in FIG.

As shown in FIG. 4, the total lipid content of the red lipids (Example 1) was lower than that of the red lipid powder (Comparative Example 1), and the total lipid content of the red lipids 2) showed the lowest total cholesterol release than the other samples (Comparative Examples 1 to 5).

[ Experimental Example  3] Let's  Used animal experiment

The control group and the treatment group were fed diets containing high cholesterol diet (Research Diets, D12336, containing 12.5% cholesterol and 16% fat) for 4 weeks to SD (sprague dawley) Resulting in arteriosclerosis. One week after the adaptation period, And the control group was provided with water. In the treatment group, red lupine-containing natural liposome of Example 1, red lupe-containing natural liposome of Example 2 and natural liposome containing red ginseng, red yeast powder of Comparative Example 1, The natural liposome containing red ginseng, the red ginseng extract of Comparative Example 3, and the gamma linolenic acid of Comparative Example 4 were fed. After the blood was collected from the rats, the liver tissue was dissected out.

(1) Check blood

The collected blood was centrifuged at 5000 rpm for 10 minutes at room temperature to separate the serum and used.

Absorbance was measured at 550 nm with a spectrophotometer using a reagent for measuring triglyceride (AS15, manufactured by Asan Pharmaceutical Co., Ltd., AM157S-k) in order to confirm the content of triglyceride (TG).

Absorbance was measured at 500 nm with a spectrophotometer using a total cholesterol measuring reagent (AM202, manufactured by Asan Pharmaceutical Co., Ltd.) in order to determine the total cholesterol amount, and the results are shown in Fig.

In order to confirm the HDL cholesterol level, it is shown in the reagent for measuring HDL cholesterol (Asan Pharmaceutical Co., Ltd., AM203-k).

The LDL cholesterol level was measured with an automatic clinical analyzer (HITACHI, 7600) using LDL-C plus (Roche, Cat No. 04711220-190) for LDL cholesterol measurement, and the results are shown in FIG.

As shown in Figs. 5 to 8, when the natural liposome containing red yeast (Example 1) was fed, the content of triglyceride, total cholesterol and LDL cholesterol was lower than that of red yeast powder (Comparative Example 1) Respectively.

In addition, when the mixture of the natural liposome containing red yeast and the natural liposome containing red ginseng (Example 2) was fed, the content of triglyceride, total cholesterol and LDL cholesterol was lower than those of other samples (Comparative Examples 1 to 5) Cholesterol content was similar.

Thus, it was confirmed that the mixture of the natural liposome containing red yeast (Example 1) or the natural liposome containing red yeast and the natural liposome containing red ginseng (Example 2) had an effect of improving the blood lipid composition.

In order to measure the AST value, the absorbance at 505 nm was measured with a photoelectric colorimetric system using an AST measurement reagent (Asan Pharmaceutical Co., Ltd., AM103-k). The results are shown in FIG.

In order to measure the ALT value, the absorbance at 505 nm was measured with a photoelectric colorimetric system using an ALT measurement reagent (Asan Pharmaceutical Co., Ltd., AM102-k). The results are shown in FIG.

As shown in FIGS. 9 and 10, the total ALT and AST contents of the red algae-containing natural liposome (Example 1) were lower than those of the red algae powder (Comparative Example 1).

In addition, the lowest ALT and AST values were obtained when the mixture of the natural liposome containing red yeast and the natural liposome containing red ginseng (Example 2) was fed.

Therefore, it was confirmed that the mixture of the natural liposome containing red yeast (Example 1) or the natural liposome containing red yeast and the natural liposome containing red ginseng (Example 2) was low in liver damage.

(2) Confirmation of liver tissue

The extracted liver tissue was immersed in 10% formalin, liver tissue was transferred to a cassette and washed with distilled water. Liver tissues were sequentially dehydrated in 70% ethanol, 80% ethanol, 90% ethanol, 95% ethanol and 100% ethanol. To remove the ethanol remaining in liver tissue, xanthrene was immersed in xylene three times and the liver tissue block was made with dissolved paraffin solution. The liver tissue block was cut at 3 μm using a microtome and transferred to a slide. Subsequently, the tissues of the slide were sequentially immersed in xylene, 100% ethanol, 95% ethanol, 80% ethanol, 70% ethanol and distilled water to remove the paraffin. After immersing in a hematoxylin solution for 10 minutes, it is washed with distilled water for 5 minutes, immersed in 1% hydrochloric acid-ethanol (HCl-EtOH) 5 times, and then washed with distilled water for 3 minutes. After immersing in an eosin solution for 2 minutes, dehydrated in 70% ethanol, 80% ethanol, 90% ethanol, 95% ethanol and 100% ethanol successively. Finally, it was immersed twice in xylene. The size and area of lipid spheres were observed with an optical microscope, and the results are shown in Fig.

As shown in Fig. 11, when the natural liposome containing red ginseng (Example 1) was fed, and the mixture of natural red liposome containing red ginseng and the natural liposome containing red ginseng (Example 2) 5) showed little lipid production.

Liver tissues were extracted and immediately embedded in OCT compound for frozen section and cooled at -80 ° C. The cooled liver tissue was sectioned at a size of 7 [mu] m using a cryostat as a cryostat. After staining, the slides were air dried for 30 minutes at room temperature. The dried slides were immersed in 10% formalin for 5 minutes and then the slides were washed three times with distilled water filtered on ice. Rinsed with 85% propylene glycol for 5 minutes at 60 &lt; 0 &gt; C, washed again with filtered distilled water, and mounted with 20% glycerol PBS. The size and area of the lipid stained red with a microscope were observed, and the results are shown in FIG.

As shown in Fig. 12, when the natural liposome containing red ginseng (Example 1) was fed and the mixture of natural red liposome containing red yeast and the natural liposome containing red ginseng was administered (Example 2) 5) showed little lipid production.

Claims (22)

A liposome-forming material containing at least one natural emulsifier, a natural-derived solvent, a natural coating agent and a natural preservative; And
Hongkuk extract as a capturing substance
/ RTI &gt;
Wherein the natural emulsifier, the natural-derived solvent, and the red lotus extract are ultrasonicated and mixed. The method according to claim 1,
Based on the total weight of the red rice bran-containing natural liposome. Containing natural liposome containing 0.1 to 10% by weight of the natural emulsifier, 0.001 to 3% by weight of the natural coating agent, 0.001 to 3% by weight of the natural preservative, 0.1 to 30% by weight of the Hong Kong extract, and the remaining amount of the natural derived solvent. The method according to claim 1,
Natural liposome containing red yeast for improving lipid and blood circulation. (a) a first step of mixing at least one natural emulsifier with a natural-derived solvent and sonicating at 35 to 80 캜;
(b) adding a red gingko extract to the mixture of the first step and ultrasonically treating the mixture at 35 to 80 캜 for mixing;
(c) a third step of adding a natural coating agent to the mixture of the second step and ultrasonication at 35 to 80 캜; And
(d) adding a natural-derived solvent and a natural preservative to the mixture of the third step and ultrasonically treating the mixture at 35 to 80 캜
4. The method according to claim 1, wherein the lipid is a lipid. 5. The method of claim 4,
And further comprising a fifth step of repeatedly ultrasonifying the solution while gradually lowering the temperature after the fourth step. A food composition comprising, as an active ingredient, a natural liposome containing red lips according to any one of claims 1 to 3 as an active ingredient or a natural liposome containing red lips and a red lip gloss containing red ginseng. The method according to claim 6,
Wherein the red ginseng-containing natural liposome comprises at least one natural emulsifier, a natural-derived solvent, a natural coating agent and a natural preservative; And
And a red ginseng extract as a capturing substance. The method according to claim 6,
Wherein the natural liposome containing red ginseng is formed by ultrasonication and mixing the natural emulsifier, the natural-derived solvent and the red ginseng extract. The method according to claim 6,
Wherein the composition further comprises one or more extracts selected from the group consisting of red ginseng extract, red ginseng extract, garlic extract, onion extract, cinnamon extract, 칡 extract, bamboo extract, and soybean extract. 10. The method of claim 9,
Wherein the food composition comprises 1 to 40% by weight of the red noodle-containing natural liposome, 10 to 50% by weight of the natural red liposome containing the red ginseng, and 5 to 50% by weight of the extract based on the total weight of the food composition. The method according to claim 6,
Food composition for improving lipid and blood circulation. 8. The method of claim 7,
The natural emulsifier contained in the red-noodle-containing natural liposome and the natural emulsifier contained in the red-lipid-containing natural liposome are the same or different from each other,
Wherein the natural emulsifier is at least one selected from the group consisting of lecithin, phosphatidyl choline, lysophosphatidyl choline, phosphatidyl ethanolamine, hydronephosphatidyl choline, phosphatidic acid, phosphatidylserine, phosphatidyl glycerol, phosphatidylinositol, Phospholipids. &Lt; / RTI &gt; 8. The method of claim 7,
The natural emulsifier contained in the red-noodle-containing natural liposome and the natural emulsifier contained in the red-lipid-containing natural liposome are the same or different from each other,
Wherein the natural emulsifier comprises at least one fatty acid selected from the group consisting of palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid. 8. The method of claim 7,
The natural-derived solvent contained in the red-noodle-containing natural liposome and the natural-derived solvent contained in the natural liposome containing red gins are the same as or different from each other,
Wherein the natural-derived solvent is at least one solvent selected from the group consisting of distilled water, butylene glycol, propanediol, glycerin, and fermented alcohol. 8. The method of claim 7,
The natural absorption promoter contained in the red lentil-containing natural liposome and the natural absorption promoter contained in the red lipid liquid containing the red ginseng are the same or different from each other,
Wherein the natural coating agent is at least one selected from the group consisting of guarana extract and natural derived dextrin. 8. The method of claim 7,
The natural preservative contained in the red lentil-containing natural liposome and the natural preservative contained in the natural liposome containing red ginseng are the same or different,
Wherein the natural preservative is one or more natural extracts selected from the group consisting of citrus extract, citron extract, eucalyptus extract, gossam extract, clove extract, grapefruit extract, cherry pine fruit extract, horseradish flower extract and earth flower extract. A pharmaceutical composition comprising, as an active ingredient, a natural liposome containing red yeast according to any one of claims 1 to 3 as an active ingredient, or a natural liposome containing red yeast containing the red yeast and red ginseng. 18. The method of claim 17,
Wherein the red ginseng-containing natural liposome comprises at least one natural emulsifier, a natural-derived solvent, a natural coating agent and a natural preservative; And
And a red ginseng extract as a capturing substance. 19. The method of claim 18,
Wherein the natural liposome containing red ginseng is formed by ultrasonication and mixing the natural emulsifier, the natural-derived solvent and the red ginseng extract. 18. The method of claim 17,
Wherein the extract further comprises one or more extracts selected from the group consisting of red ginseng extract, red ginseng extract, garlic extract, onion extract, cinnamon extract, 칡 extract, white rye extract and soybean extract. 21. The method of claim 20,
A pharmaceutical composition comprising 1 to 40% by weight of the red lentil-containing natural liposome, 10 to 50% by weight of the natural red liposome containing the red ginseng, and 5 to 50% by weight of the extract based on the total weight of the pharmaceutical composition. 18. The method of claim 17,
A pharmaceutical composition for improving lipid and blood circulation.

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