KR20170106772A - Pseudomonas sp. DJ15 with Insecticidal Activity against Aphid - Google Patents

Abstract

Provided are a novel Pseudomonas sp. DJ15 strain (KACC 92116P) having controlling effects on aphid, and a composition for controlling aphid using the same. According to the present invention, the novel Pseudomonas sp. DJ15 strain and xantholysin A and B isolated from a culture solution thereof exhibit excellent controlling effects on aphid, and the composition for controlling aphid is an eco-friendly biological composition with little adverse effects on environmental pollution and the ecosystem.

Description

A novel Pseudomonas sp. DJ15 strain {Pseudomonas sp. DJ15 with Insecticidal Activity against Aphid}

The present invention relates to a novel Pseudomonas sp. DJ15 strain having an aphid control activity.

Aphids are one of the main pests of agriculture that occur continuously throughout the year, and their resistance to drugs is high due to the short repetition of generations. Plant diseases caused by aphid-mediated vegetal viruses and the nectar secreted by these insects significantly reduce crop production. Therefore, farmers use synthetic pesticides as a representative to control aphids. However, since the use of synthetic pesticides may cause adverse effects on environment and manpower, research is being conducted on other control methods. As an alternative to synthetic pesticides, natural pesticides and microbial pest control can be mentioned. Studies on the development of natural plant preparations have been actively carried out, but there have been very few studies on microbial preparations. Therefore, it is necessary to study the development of aphid control method that can be applied directly to the farm site in addition to the securing of microbial resources for the control of aphid.

Therefore, the present inventors have found that the possibility of harmful environmental pollution and ecosystem adversely affecting the aphid of aphids is low and the resistance to the aphids is low, In addition to securing these excellent microorganisms, we have continued to develop a method for controlling and controlling the peach aphid by using the same.

Conventionally, a method of spraying a machine oil emulsion to the winter or spraying aphid drug for the early stage of development is used for controlling the peach aphid. Commercially available antiseptic agents include Atala granules, chess granular granules, Atara granular granules, therapeutic granules, balloam fresh liquid waxes, and aerosols / emulsions.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.

KR Patent No. 10-1444214 KR Patent No. 10-0404767 KR Patent No. 10-0940231

The present inventors have sought to isolate a new indigenous microorganism having an aphid control activity and its active substance. As a result, the present invention was completed by isolating a novel Pseudomonas sp. DJ15 strain having a controlling activity against aphids and confirming the aphid control activity of xantholysin A and B, which are active substances for controlling the aphid.

Accordingly, an object of the present invention is to provide a novel Pseudomonas sp. DJ15 strain (KACC 92116P).

It is another object of the present invention to provide a composition for controlling aphids.

It is another object of the present invention to provide a method for controlling aphids.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, there is provided a novel Pseudomonas sp. DJ15 strain (KACC 92116P) having an aphid control activity.

The present inventors have sought to isolate a new indigenous microorganism having an aphid control activity and its active substance. As a result, a novel Pseudomonas sp. DJ15 strain having a controlling activity against aphids was isolated, and the aphid control activity of xantholysin A and B, which are active ingredients of the Pseudomonas sp.

The Pseudomonas sp. DJ15 strain according to the present invention is a domestic indigenous microorganism isolated from seaside soils and has a characteristic of producing insecticidal substances against aphids.

The novel Pseudomonas sp. DJ15 strain (KACC 92116P) of the present invention has a controlling activity against aphids.

According to one embodiment of the present invention, the aphid is a peach aphid ( Myzus persicae ).

The peach lycopodium aphid is a pest which causes a direct damage by stopping the growth of a plant by the sucking of the juice by attaching adults or nymphs to the back of young leaves or leaves. It also causes indirect damage by transferring hundreds of plant virus diseases. Leaves and stems, as well as flowers and fruits, such as parasitic by sucking the juice by eating the plant nutrients are lost, and the leaves are distorted, such as shriveling or drying. It also secretes nectar from faeces and causes soot disease. When soot disease occurs, the leaves become dark, photosynthesis is inhibited, and the plant becomes fragile. Secondary damage causes viruses such as PLRV (potato leaf blight virus), TuMV (turnip mosaic virus), CAMV (flowering cabbage mosaic virus) and CMV (cucumber mosaic virus) to be transferred to crops.

As verified in the following examples, the Pseudomonas sp. DJ15 (KACC 92116P) strain secretes an aphid control active substance.

According to one embodiment of the present invention, the Pseudomonas sp. DJ15 (KACC 92116P) strain produces at least one xanthoraicin selected from the group consisting of xanthorrhizin A and xanthorhindin B.

The Pseudomonas sp. DJ15 (KACC 92116P) strain of the present invention produces Zanthorrhizin A of the following formula (1).

[Chemical Formula 1]

The Pseudomonas sp. DJ15 (KACC 92116P) strain of the present invention produces Zantraisin B of the following formula (2).

(2)

Since the strain Pseudomonas sp. DJ15 (KACC 92116P) produces zotorhionin A and / or zantholysin B having an aphid control activity, all of the above strains and their cultures have a controlling activity against aphids.

According to one embodiment of the present invention, the xanthoraicin A exhibits a half-life lethal dose (Lethal Dose 50. LD ₅₀ ) at a concentration of 22-26 mg / L.

According to another embodiment of the present invention, the xanthorhythin A exhibits a half-life mortality at a concentration of 23-26 mg / L, 24-26 mg / L or 24-25 mg / L.

According to one embodiment of the present invention, the xanthorrhizin B exhibits a half-life lethal dose at a concentration of 10-15 mg / L.

According to another embodiment of the present invention, the xanthorhythin B exhibits a half lethal dose at a concentration of 11-15 mg / L, 12-15 mg / L, 13-15 mg / L or 13-14 mg / L.

The fact that the xanthorrhizin has an aphid control activity is the result of the first identification in the present invention.

The novel Pseudomonas sp. DJ15 strain (KACC 92116P) of the present invention has a chitinolytic activity.

As demonstrated in the following examples, the Pseudomonas sp. DJ15 strain of the present invention secretes chitinase capable of degrading chitin.

According to another aspect of the present invention, the present invention provides a method for producing a Pseudomonas sp. DJ15 (KACC 92116P), a culture of the Pseudomonas sp. DJ15 (KACC 92116P), an extract of the culture solution, xantholysin A and xantholysin B The present invention provides a composition for controlling aphids, comprising at least one component selected from the group consisting of

According to still another aspect of the present invention, there is provided a method of controlling aphids, comprising spraying or applying the composition for controlling aphids.

Crops to which the composition of the present invention can be applied include food crops including rice, wheat, barley, corn, soybeans, potatoes, wheat, red beans, oats and millet; Vegetable crops including Arabidopsis, cabbage, radish, pepper, strawberry, tomato, watermelon, cucumber, cabbage, melon, squash, onions, onions and carrots; Special crops including ginseng, tobacco, cotton, sesame, sugar cane, beet, perilla, peanut and rapeseed; Apple trees, pears, jujubes, peaches, sheep, grapes, citrus, persimmon, plums, apricots and banana; Roses, gladiolus, gerberas, carnations, chrysanthemums, lilies and tulips; And feed crops including ragras, red clover, orchardgrass, alpha-alpha, tall fescue and perennialla grass.

Since the composition for controlling aphids and aphid control method of the present invention uses the novel Pseudomonas sp. DJ15 strain (KACC 92116P), the description common to both of them is omitted in order to avoid the excessive complexity of the present specification.

The features and advantages of the present invention are summarized as follows:

(a) The present invention provides a novel Pseudomonas sp. DJ15 strain having a controlling activity against aphids and a chitin-degrading activity, and a composition for controlling aphids using the same.

(b) The novel Pseudomonas sp. DJ15 strain of the present invention and the zotorhizins A and B isolated from the culture thereof exhibit excellent aphid control activity.

(c) The aphid control composition of the present invention is an environmentally friendly biological composition, and is less likely to adversely affect environmental pollution and ecosystem.

Figure 1 shows a transmission electron micrograph showing the size of the novel Pseudomonas sp. DJ15 strain (KACC 92116P).
Fig. 2 shows the chitin degrading activity of the novel Pseudomonas sp. DJ15 strain.
Figure 3 shows the molecular phylogenetic location based on 16S gene sequencing analysis of the novel Pseudomonas sp. DJ15 strain.
Fig. 4 shows the peptic aphid insecticidal activity by the novel Pseudomonas sp. DJ-15 culture medium according to the culture medium.
FIG. 5 shows the result of confirming the insecticidal rate by culturing a novel Pseudomonas sp. DJ15 strain in an inorganic salt medium containing glucose, mannose, sucrose, fructose or galactose as a carbon source.
6A and 6B show results of MALDI-TOF / MS analysis of Zanthorrhizin A and B, insecticidal substances against aphids of peach aphid in the novel Pseudomonas sp. DJ15.
FIGS. 7A and 7B show the results of proton 2D TOCSY NMR analysis of Zanthorrhizin A and B, insecticidal substances against peach aphid in New Pseudomonas sp. DJ15.
Fig. 8 shows the formulas of the novel Pseudomonas sp. DJ15-derived zotolacins A and B; (1) is the xanthoraicin A, and the formula (2) is the formula of the xanthoraicin B.
9A and 9B show the mortality of peach aphid according to the concentrations of Zanthorrhizin A and B, insecticidal substances produced by the Pseudomonas sp. DJ15 strain of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Here, unless otherwise defined in the technical terms and scientific terms used, those having ordinary skill in the art to which the present invention belongs have a generally understood meaning. Repeated descriptions of the same technical constitution and operation as those of the conventional art will be omitted.

Example 1: Isolation and culture of insecticidal active microorganisms

1 g of soil collected from Gochang Beach was placed in CY medium and cultured for 5 days. At this time, the composition of the CY medium was composed of 2% Swellen chitin and 0.2% yeast extract. The culture was inoculated at a level of 1% (v / v), and a portion of the culture was plated on CY agar medium (2% swell chitin, 0.2% yeast extract, 2% agar) and cultured at 30 ° C.

After 3 days, the colonies on the CY agar medium were cultured for 96 hours in LB liquid medium (Tryptone 10.0 g, yeast extract 5.0 g, and sodium chloride 10.0 g), respectively, and the insecticidal activity against aphids was examined. Were selected. The strain with the highest activity of the selected aphid was obtained by repeated separation of subculture in an LB agar medium (10.0 g of tryptone, 5.0 g of yeast extract, 10.0 g of sodium chloride, 15.0 g of agar) and then its structure was examined by transmission electron microscope (Fig. 1). As can be seen in FIG. 1, the size of the strain was 1 to 2 (length) × 0.5 to 1 (width) μm, and the shape of the strain was a short rod shape. The purely isolated microorganism was identified as a non-pathogenic pseudomonas sp. Gram-negative bacterium in 16S ribosomal gene analysis. Further, it was confirmed that a newly identified Pseudomonas sp. Gram-negative bacterium formed a clear zone after 3 days when cultured in a CY agar medium containing chitin at 30 DEG C to confirm that it has a cleavage ability against chitin (Fig. 2).

Example 2: Identification of aphid insecticidally active microorganisms by base sequence analysis

The chromosomal DNA (chromosome DNA) was extracted for the identification of the aphid insecticidally active microorganism purely isolated in Example 1, and the gene sequence of 16S ribosomes was analyzed to confirm the nucleotide sequence of the 1st sequence of the sequence listing.

As shown in FIG. 3, the Pseudomonas mosselii CIP 105259 (AF072688) strain was taxonomically classified into a 99.7% strain as shown in FIG. 3 as a result of phylogenetic analysis by the neighbor-joining method. %.

Example 1 The purified aphids from insecticidally active microorganism Pseudomonas mosselii CIP 105259 (AF072688) strains and were similar On the phylogenetic classification based on the genetic analysis, yet Pseudomonas mosselii CIP 105259 (AF072688) strains in the aphid not the reported bar It was identified as a new microorganism having insecticidal activity and chitinolytic activity and named as DJ15 in Pseudomonas and deposited with the National Institute of Agricultural Science and Technology, National Institute of Agricultural Science and Technology and received the deposit number KACC 92116P on Jan. 13,

Example 3: Isolation and isolation of insecticidal activity of DJ-15 from Pseudomonas sp.

The new microorganism pseudomonas sp. DJ15 of Example 1 was cultured overnight in LB medium, and then a part of the culture (1%, v / v) was inoculated into an inorganic salt medium containing 0.2% glucose as a carbon source, , And shake cultured at 150 rpm for 4 days. The composition of the inorganic salt medium was 1.0 g KH ₂ PO ₄ , 2.0 g Na ₂ HPO ₄ , 0.4 g (NH ₄ ) ₂ SO ₄ , 0.4 g MgSO ₄揃 7H ₂ O and 0.2% (v / v). 0.05 g KI, 0.05 g LiCl, 0.08 g MnSO ₄ .4H ₂ O, 0.05 g H ₃ BO ₃ , 0.1 g ZnSO ₄ , 0.1 g Al ₂ O ₃ , 0.05 g SnCl ₂ .2H ₂ O, and 7H consisted ₂ O, 0.1 g CoCl ₂ and 6H ₂ O, 0.1 g NiSO ₄ and _{6H 2 O, 0.05 g BaCl 2} , 0.05 g (NH 4) 6 Mo 7 O and 4H ₂ O and 0.08 g FeSO ₄ .

The shake cultured stock solution was sprayed on aphids to investigate the mortality rate after 24 hours. The insecticidal rate was determined by Abbott's formula, insecticidal rate = {[(untreated aphid aphid aphid aphid aphid aphid aphid)] / aphid aphid aphid aphid} × 100 based on the ratio of green peach aphid Respectively. The results are shown in Fig.

As shown in FIG. 3, the aphid kill rates of the DJ-15 culture medium cultured in the LB medium, the inorganic salt medium and the CY medium were each 70% or more, indicating that the culture medium of the present invention also had an insecticidal effect , Indicating that the culture medium contained insecticidal substances. The DJ15 strain is a strain first isolated from CY medium, and the CY medium is a medium suitable for selecting a strain using chitin as a carbon source. Chitin can be isolated from the crab shell and is used as an economically superior material for commercialization. Therefore, the bacteria isolated from the CY medium can be grown by using chitin, that is, a strain having chitinolytic activity, which can be economically advantageous in commercialization. DJ15 isolated from CY medium showed about 70% insecticidal activity against aphids when cultured in CY medium. CY medium provides only minimal nutrients. In order to investigate better insecticidal rate, LB medium, which is a high-quality medium, was used. As a result, a large amount of secondary metabolites were produced due to abundant nutrients. (Fig. 4). Experiments were carried out in order to determine the optimum medium. DJ15 was cultivated in a mineral salt medium using a small amount of various carbon sources (20 g / L), showing a high insecticidal rate in glucose and mannose in carbon sources (FIG. 5). To isolate the insecticidal material, DJ15 was cultured in CY medium, inorganic salt medium (carbon source: 20 g / L of glucose) and LB medium. CY medium was excluded because it was cultured for 5-6 days at 30 ° C and 150 rpm. The culture medium of DJ15 (culture for 4 days, 30 ° C, 150 rpm) was cultured on inorganic salt medium and LB medium And purified by TLC and HPLC. Inorganic salt medium showed lower insecticidal rate than LB medium. However, in the case of DJ15 cultured in LB medium during extraction and purification, a variety of substances other than aphid insecticide substances were found, which made purification difficult. Therefore, the aphid insecticide was isolated from the culture medium in which DJ15 was cultured with an inorganic salt medium which was considered to be easier to purify and extract the insecticidal substance.

In order to investigate the insecticidal substances, the DJ15 strain cultured in a glucose mineral medium was centrifuged, and then the supernatant was extracted with ethyl acetate and chloroform. As a result of examining the aphid kill rate of the extracts, the highest activity Was investigated.

The ethyl acetate extract having the highest activity was subjected to silica gel chromatography purification. 50 g of the silica gel was wet-packed in a glass column (3.5 cm in diameter × 21 cm in length) with 100% chloroform, and the ethyl acetate extract obtained above was placed in a column. The column was washed with chloroform (CHCl ₃ ) System was eluted. The solvent system used for purification was eluted with CHCl ₃ -EtOAc = 55: 45 (v: v). Each eluate was obtained and confirmed by TLC. As a result, it was confirmed that the part which develops on TLC has insecticidal activity against peach aphid.

The active fraction obtained from the silica gel purification was further purified using a Supelclean LC18 column. The above-mentioned active fractions were put into a column and eluted with methanol, and 6 ml aliquots were collected. After collecting the eluate, the insecticidal activity was examined by examining the insecticidal activity of aphids, followed by analysis by liquid chromatography to collect a single peak compound. The column used was ODS (diameter 4.6 mm x length 20 cm), and an aqueous solution containing 84% (v / v) methanol was used as the solvent.

The active fractions purified in the liquid chromatography were concentrated and then loaded onto a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF / MS, FIG. 6) The chemical structures of the insecticidal materials (Formulas 1 and 2) were predicted by analyzing with nuclear magnetic resonance (NMR, FIG. 7) (FIG. 8).

[Chemical Formula 1]

(2)

As a result of MALDI-TOF MS analysis, as shown in FIGS. 7A and 7B, the molecular weight of [M + Na] ⁺ was 1798.0403, the molecular weight of Zantorysin A was [M + Na] ⁺ (1784.0956) were detected, respectively. This was a xanthorrhicin compound having a molecular weight similar to that previously reported (Li et al., 2013, PLoS one, 8 (5): e62946).

As a result of NMR analysis of this compound, it was confirmed that the compound has a typical tendency of the xanthorrhizine compound as shown in Table 1 below.

Peptides order HN Hα H? Other Zantoraicin A LEU1 8.44 4.22 1.65, 1.61 Hg: 1.61; Hd: 0.85 GLU2 8.72 4.20 1.94, 2.06 Hg: 2.40 GLN3 8.31 4.21 2.04 Hg: 2.28, 2.33; Hd: 7.48, 6.85 VAL4 7.67 3.99 2.18 Hg: 0.87. 0.91 LEU5 7.92 4.12 1.63 Hg: 1.63; Hd: 0.84 GLN6 8.21 4.07 1.95 Hg: 2.21; Hd: 7.28, 6.72 SER7 7.89 4.64 4.26 VAL8 8.27 4.15 2.38 Hg: 0.88, 1.00 LEU9 8.17 4.13 1.74 Hg: 1.46; Hd: 0.84, 0.77 GLN10 7.91 4.23 1.99 Hg: 2.31; Hd: 7.37, 6.74 LEU11 7.94 4.20 1.54, 1.73 Hg: 1.54; Hd: 0.88, 0.81 LEU12 7.88 4.14 1.61 Hd: 0.84 GLN13 7.24 4.10 1.87 Hg: 2.14, 2.26; Hd: 7.37, 6.96 ILE14 7.56 4.30 1.81 Hg: 1.10, 1.29; Hd: 0.78 Zantoraicin B LEU1 8.42 4.23 1.65, 1.61 Hg: 1.61; Hd: 0.85 GLU2 8.69 4.20 1.94, 2.06 Hg: 2.4 GLN3 8.30 4.22 2.04 Hg: 2.30; Hd: 7.47, 6.86 VAL4 7.668 4.00 2.18 Hg: 0.89 LEU5 7.91 4.13 1.62 Hg: 1.62; Hd: 0.84 GLN6 8.22 4.08 1.95, 2.13 Hg: 2.21; Hd: 7.27, 6.72 SER7 7.90 4.66 4.27 VAL8 8.27 4.14 2.36 Hg: 0.88, 0.99 LEU9 8.12 4.16 1.73, 1.45 Hg: 1.45; Hd: 0.83 GLN10 7.91 4.23 2.00 Hg: 2.30; Hd: 7.36, 6.73 LEU11 7.99 4.18 1.54, 1.72 Hg: 1.54; Hd: 0.88, 0.80 LEU12 7.87 4.15 1.61 Hd: 0.84 GLN13 7.35 4.12 1.87 Hg: 2.15, 2.27; Hd: 7.38, 6.85 VAL14 7.63 4.24 2.04 Hg: 0.79

Example 4: Aphid insect test of Zanthorrhizin A and B isolated from Pseudomonas spp DJ-15

Zymoliticins A and B isolated from the Pseudomonas sp. DJ-15 cultures of Formulas 1 and 2 were sprayed on the leaf surface of Chinese cabbage leaflets transplanted with second-day peach aphids, and then examined for aphid kill rate after 24 hours. As shown in FIGS. 9a and 9b, when the aphid insecticidal rate was examined, the zotolite A was found to have a rate close to 100% and the half-life lethal dose was 24.6 mg / L when treated with 50 mg / L or more. Zanthorrhizin B showed a close to 100% aphid kill rate at 20 ㎎ / L or more, and the half-life lethal dose was 13.4 mg / L.

These results are the result of confirming that Zanthorrhizin produced by Pseudomonas sp. DJ15 of the present invention can be used as aphid control agent. Thus, it is the first result of the present invention that Zanthorrhizin has aphid insecticidal power.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Agricultural Biotechnology Research Institute KACC92116P 20160113

<110> INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY <120> Novel Pseudomonas sp. DJ15 Strain having Control Activity against          Aphid <130> PN160069 <160> 1 <170> Kopatentin 2.0 <210> 1 <211> 1419 <212> RNA <213> Pseudomonas sp. DJ15 16s rRNA <400> 1 ctacacatgc agtcgagcgg atgacgggag cttgctcctt gattcagcgg cggacgggtg 60 agtaatgcct aggaatctgc ctggtagtgg gggacaacgt ttcgaaagga acgctaatac 120 cgcatacgtc ctacgggaga aagcagggga ccttcgggcc ttgcgctatc agatgagcct 180 aggtcggatt agctagtagg tgaggtaatg gctcacctag gcgacgatcc gtaactggtc 240 tgagaggatg atcagtcaca ctggaactga gacacggtcc agactcctac gggaggcagc 300 agtggggaat attggacaat gggcgaaagc ctgatccagc catgccgcgt gtgtgaagaa 360 ggtcttcgga ttgtaaagca ctttaagttg ggaggaaggg cagtaagtta ataccttgct 420 gtttggggt taccgacaga ataagcaccg gctaactctg tgccagcagc cgcggtaata 480 cagagggtgc aagcgttaat cggaattact gggcgtaaag cgcgcgtagg tggttcgtta 540 agttggatgt gaaagccccg ggctcaacct gggaactgca tccaaaactg gcgagctaga 600 gtatggtaga gggtggtgga atttcctgtg tagcggtgaa atgcgtagat ataggaagga 660 acaccagtgg cgaaggcgac cacctggact gatactgaca ctgaggtgcg aaagcgtggg 720 gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcaa ctagccgttg 780 gaatccttga gattttagtg gcgcagctaa cgcattaagt tgaccgcctg gggagtacgg 840 ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca caagcggtgg agcatgtggt 900 ttaattcgaa gcaacgcgaa gaaccttacc aggccttgac atgcagagaa ctttccagag 960 atggattggt gccttcggga actctgacac aggtgctgca tggctgtcgt cagctcgtgt 1020 cgtgagatgt tgggttaagt cccgtaacga gcgcaaccct tgtccttagt taccagcacg 1080 tcatggtggg cactctaagg agactgccgg tgacaaaccg gaggaaggtg gggatgacgt 1140 caagtcatca tggcccttac ggcctgggct acacacgtgc tacaatggtc ggtacagagg 1200 gttgccaagc cgcgaggtgg agctaatctc acaaaaccga tcgtagtccg gatcgcagtc 1260 tgcaactcga ctgcgtgaag tcggaatcgc tagtaatcgc aaatcagaat gttgcggtga 1320 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaccagaa 1380 gtagctagtc taaccttcgg gaggacggta tacatcggt 1419

Claims (7)

A novel Pseudomonas sp. DJ15 strain (KACC 92116P) having an antifungal activity and chitinolytic activity.
The strain of claim 1, wherein the aphid is Myzus persicae .
The strain of claim 1, wherein the Pseudomonas sp. DJ15 (KACC 92116P) strain produces at least one xanthoraicin selected from the group consisting of xanthorrhizin A and xanthorhindin B.
4. The strain according to claim 3, wherein the Zanthoraicin A exhibits a half-life lethal dose (Lethal Dose 50) at a concentration of 22-26 mg / L.
4. The strain according to claim 3, wherein the Zanthoraicin B exhibits a half-life (Lethal Dose 50) at a concentration of 10-15 mg / L.
At least one component selected from the group consisting of Pseudomonas sp. DJ15 (KACC 92116P), the Pseudomonas sp. DJ15 (KACC 92116P), the culture solution extract, xantholysin A and xantholysin B Or a pharmaceutically acceptable salt thereof.
A method for controlling aphids, comprising spraying or applying the composition for controlling aphids of claim 6.

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