KR20170104817A - Slide for single cell detection and picking - Google Patents

Abstract

Disclosed is a slide for detecting and collecting a single cell. The disclosed slide is used for the detection and collection of objects used in an analyzer, and includes a protrusion unit protruding upwardly from the surface of a support and having an opened structure. The opened region of the protrusion unit may include a blocking unit which prevents the leakage of a solution in the region surrounded by the protrusion unit.

Description

Slide for single cell detection and picking

The present disclosure relates to single cell detection and collection slides and relates to a slide used for detecting or collecting an object from a sample.

Generally, when an object such as a cell is to be detected or collected from a living body, a device such as a microscope or a micromanipulator is used. It is important to select only the analysis target and collect it accurately while observing the solution containing the analysis target using the analysis equipment.

For example, in the study of circulating tumor cells, nucleated red blood cells, embryonic cells or stem cells, it should be possible to identify and selectively collect from solutions containing such cells, for example body fluids. To do this, research equipment must be equipped to identify and collect analytes selectively from the solution.

In one aspect of the invention, there is provided a single cell detection and collection slide having some open end wall structure. And a single cell detection and collection slide including an end region formed in an open area of the slide structure such that solution is not discharged outside the barrier wall of the slide structure.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS In this disclosure, for a single cell detection and collection slide used in an analytical apparatus,

support fixture; And

And a protrusion protruded upward from the surface of the support,

Wherein the protruding portion has a shape in which at least one side is open,

In the region surrounded by the protrusions, a slide is provided which can include an object to be analyzed by the analysis device.

The protrusion includes a first protrusion formed in a first direction; And

And a second protrusion formed in a second direction different from the first direction.

The protrusion may include a third protrusion formed in a third direction identical to the first direction.

The first protrusion and the third protrusion may be opposed to each other and opposed to each other.

The second protrusion may be curved.

And a blocking portion formed on the support on at least one side of the protrusion.

The blocking portion may be relatively wettable relative to the surface area of the support enclosed by the protrusion.

The blocking portion may have a hydrophobic property.

The blocking portion may be formed of an organic solvent.

The blocking portion may be formed by self-assembled monolayer (SAM) formed by a microchannel (CH3) or silane process, by coating with Sigmacote (chlorinated organopolysiloxane in heptane) or by using a PAP pen (proprietary component) Marking or treated with paraffins.

The surface area of the support enclosed by the protrusions may have a hydrophilic character.

The surface area of the support frame surrounded by the protrusion may be a plasma treatment using oxygen plasma or a corona discharge treatment.

The surface area of the support enclosed by the protrusions may be a self-assembled monolayer (SAM) formed by a carboxyl group (COOH) treatment or a Biolipidure 405® (Proprietary component) coating.

According to the present disclosure, it is possible to provide a single cell detection and collection slide capable of selectively collecting a target by analyzing a solution containing the analysis target. It is possible to prevent the formation of a dead zone in the support by forming a protrusion in an open form on the support of the slide structure. It is possible to prevent the solution from leaking to the outside of the protrusion by forming a blocking portion in the open region of the protrusion. In addition, the slide structure according to the disclosure can store a relatively large amount of the solution without shaking even in the movement of the stage, as compared with a slide in which a general protrusion is not formed. In addition, the slide structure according to the disclosure may be carrying a solution without evaporation or shaking even when a long time is required to distinguish the object.

Brief Description of the Drawings Figure 1 shows a microscope apparatus comprising a single cell detection and collection slide according to an embodiment.
2A is a perspective view showing a single cell detection and collection slide according to an embodiment.
FIG. 2B is a plan view of a single cell detection and collection slide according to an embodiment. FIG.
FIGS. 3A through 3C are views showing various examples of single cell detection and collection slides according to an embodiment of the disclosure; FIG.
4A is a perspective view showing collecting a sample using a pipette in a liquid contained in a single cell detection and collection slide according to an embodiment.
4B is a cross-sectional view taken along the line A-A 'in FIG. 4A.
5A is a perspective view illustrating collecting a sample using a pipette in a liquid contained in a single cell detection and collection slide including a blocking part formed in an open area according to an embodiment.
5B is a cross-sectional view taken along line B-B 'of FIG. 5A.
FIG. 6A is a perspective view showing collecting a sample using a pipette in a liquid contained in a single cell detection and collection slide having a closed structure. FIG.
6B is a cross-sectional view taken along line C-C 'of FIG. 6A.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Reference will now be made in detail to the single-cell detection and collection slides according to embodiments of the present invention, with reference to the accompanying drawings. In the following drawings, like reference numerals refer to like elements, and the size of each element in the drawings may be exaggerated for clarity and convenience of explanation.

Furthermore, the embodiments described below are merely illustrative, and various modifications are possible from these embodiments. Also, in the layer structures described below, the expression "upper" or "upper" may include not only being directly on, but also non-contacting.

Brief Description of the Drawings Figure 1 shows an analytical apparatus comprising a single cell detection and collection slide according to an embodiment.

Referring to FIG. 1, the slide structure 100 may be mounted at a predetermined location on the substructure 10 so that it can be viewed and analyzed by the analysis device 18. The slide structure 100 may have a structure including a flat support 12 and a protrusion 14 formed on the support 12.

The analyzer 18 may be, for example, a microscope, and the microscope is not limited to an inverted microscope or an upright microscope. When the analyzer 18 is a microscope, the slide structure 100 may be mounted at a position corresponding to the focal length of the objective lens of the analyzer 18 and the lower structure 10 may be mounted on a stage of the microscope Can be mounted and observed. At this time, the observer P can detect and collect the object by observing the solution containing the object on the supporter 12 of the slide structure 18 through the analyzing device 18. Fig. The observer P can detect or collect the object from the solution using the detector 16 while observing the solution containing the object located in the slide structure 18. [ The detector 16 may be a micropipette or a spit.

2A is a perspective view showing a single cell detection and collection slide according to an embodiment. FIG. 2B is a plan view of a single cell detection and collection slide according to an embodiment. FIG.

2A and 2B, the slide structure 100 may be a structure including a flat support 12 and a protrusion 14 formed on the support 12. The protrusion 14 of the slide structure 100 may have a shape protruding upward on the support 12 and may include a plurality of protrusions 14 formed in different directions.

The protrusions 14 are formed in a first direction, for example, an x-direction, and include a first protrusion 15a and a third protrusion 15c opposed to each other, For example, a second projection 15b formed in the y-direction. The protrusion 14 including the first protrusion 15a to the third protrusion 15c may have a rectangular shape with one side opened on the surface of the support base 12 having a "C" shape. The slide structure 100 according to the embodiment may have a structure in which a protrusion 14 having a region R11 at least partially opened on the support base 12 is formed. The solution containing the analyte can be inserted on the support 12 of the region surrounded by the first protrusion 15a, the second protrusion 15b and the third protrusion 15c.

Between the first projection 15a and the third projection 15c, the length of W1 or L of the region into which the solution containing the object is inserted, where W1 and L may each be a length of several millimeters to 10 centimeters have. In FIGS. 2A and 2B, the shape of the region surrounded by the protruding portion 14 is shown to be rectangular. However, the shape of the region is not limited thereto, and may have a shape in which a part of an ellipse or a polygon is removed. have.

Figures 3A-3C illustrate various examples of single cell detection and collection slides 100 in accordance with the teachings of the disclosure.

1 and 3A to 3C, the slide structure 100 includes projections 14a, 14b and 14c formed on supports 12a, 12b and 12c and supports 12a, 12b and 12c respectively Structure. The supports 12a, 12b, 12c may have a rectangular shape as shown in Figs. 3A and 3B, and may have a circular or elliptical shape as shown in Fig. 3C. The supports 12a, 12b and 12c can be determined in shape and size according to the analyzer 18 in which the slide structure 100 is used. The supports 12, 12a, 12b, and 12c may be formed of glass, plastic, or polymer-based materials, and may be formed of a material having high light transmittance.

The protrusions 14a, 14b, and 14c may include straight and curved shapes as shown in Fig. 3A, or polygonal shapes including a plurality of straight shapes having different directions as shown in Fig. 3B. The areas of the supports 12, 12a, 12b and 12c surrounded by the projections 14a, 14b and 14c may not be closed spaces but may have areas R12, R13 and R14 of which one side is open.

The protrusions 14, 14a, 14b, and 14c may be formed of glass, plastic, or polymer-based materials, and may be formed of a material having high light transmittance. The protrusions 14, 14a, 14b and 14c may be formed of the same material as the supports 12, 12a, 12b and 12c and the protrusions 12, 12a, 12b, and 12c. For example, in the process of forming the supports 12, 12a, 12b, and 12c from glass, plastic, or polymer materials, predetermined regions of the surfaces of the supports 12, 12a, 12b, , 14a, 14b, 14c can be formed. If the analyzer 18 is a microscope, it may be formed vertically upward with respect to the surfaces of the supports 12, 12a, 12b, 12c to maintain a constant distance from the objective lens with respect to the focal length with the objective lens. The heights of the protrusions 14, 14a, 14b, and 14c can be selectively determined depending on the type of solution including the analyzer 18 or the object to be analyzed, and there is no limitation. For example, the protrusions 14, 14a, 14b, 14c may be formed with a height of 0.1 millimeters to 1 cm.

4A is a perspective view showing collecting a sample using a pipette in a liquid contained in a single cell detection and collection slide according to an embodiment. 4B is a cross-sectional view taken along the line A-A 'in FIG. 4A.

Referring to Figs. 1, 4A and 4B, it is possible to include a protrusion 14 having one side opened on the surface of the support 12, and within the region surrounded by the protrusion 14, the object 22a, 22b can be accommodated. The objects 22a and 22b may be biological cells such as blood tumor cells, nucleated red blood cells, embryonic cells, or stem cells as a substance to be analyzed by the analyzer 18. And, the solution 20 may be a bodily fluid containing such cells. When the observer P wants to observe and collect the objects 22a and 22b of the slide structure 100 through the analyzer 18, the ends of the detectors 16a and 16b are moved to the position where the objects 22a and 22b Access to existing locations should be possible. Even when the objects 22a and 22b are located in the edge region of the solution 20, that is, in the region adjacent to the protrusion 14, the object 22a and 22b can be easily detected and detected without any limitation on the angles of the detectors 16a and 16b. It can be collected. A structure including a projection having a closed structure will be described with reference to Figs. 6A and 6B.

FIG. 6A is a perspective view showing collecting a sample using a pipette in a liquid contained in a single cell detection and collection slide having a closed structure. FIG. 6B is a cross-sectional view taken along line C-C 'of FIG. 6A.

6A and 6B, the slider structure 120, 140 may include a protrusion 140 protruding from the surface of the support 120 on the surface of the support 120. The protrusion 140 has a closed shape and the solution 200 containing the objects 220a and 220b is accommodated in the support surface 120 surrounded by the protrusion 140. [ The ends of the detectors 160a and 160b should approach the objects 220a and 220b in the solution 200 in order to detect and collect the objects 220a and 220b in the solution 200 accommodated in the slide structures 120 and 140 . However, if the protrusion 140 has a closed configuration, there may be a dead zone (R2) where the detectors 160a and 160b are difficult to access. For example, when the analyzer for analyzing the objects 220a and 220b is a microscope, the solution is positioned at the focal length of the objective lens, and the angle at which the detectors 160a and 160b approach the protrusion 140 is limited have. Thus, even though the detectors 160a and 160b are difficult to access, some of the regions adjacent to the protrusions 140 are in close proximity to each other, even though the observer is aware of the positions of the objects 220a and 220b in the solution 200 through the analyzer It is difficult to detect and collect the object 200b existing in the dead zone R2.

1, 4A and 4B, the slide structure 100 according to the embodiment has the projections 14, 14a, 14b and 14c formed on the supports 12, 12a, 12b and 12c, The objects 22a and 22b of the solution 20 contained in the region surrounded by the protrusions 14 and 14a and 14b and 14c are detected and collected by the detectors 16a and 16b, .

FIG. 5A is a perspective view illustrating sampling of a liquid contained in a slide structure including a blocking portion formed in an open region according to an embodiment using a pipette. FIG. 5B is a cross-sectional view taken along line B-B 'of FIG. 5A.

Referring to Figures 1, 5A and 5B, a protrusion 14 having a side opening or open area on the surface of the support 12 may be included, and within the area surrounded by the protrusion 14, 22a, 22b) can be accommodated. A blocking portion 30 is formed on the surface of the support 12 of the region opened by the protrusion 14 to prevent the solution 20 contained in the region surrounded by the protrusion 14 from escaping to the outside of the protrusion 14 .

The blocking portion 30 may be a region having a relatively low wettability in comparison with the surface region of the support 12 surrounded by the projections 14 in which the solution 20 is present. In this case, it is possible to prevent the solution 20 present in the surface region of the support table 12 in the region surrounded by the protrusion 14 from escaping to the outside of the protrusion 14. The blocking portion 30 may also be hydrophobicized to prevent the solution 20 from escaping through the blocking portion 30 from the region surrounded by the protrusion 14 to the outside. It is also possible to prevent the solution 20 from escaping out of the region surrounded by the protruding portion 14 by subjecting the region surrounded by the protruding portion 14 to a hydrophilic treatment.

For the hydrophobic treatment of the blocking portion 30, the blocking portion 30 outside the region surrounded by the protruding portion 14 may be coated with an organic solvent. The organic solvent may be, for example, a material such as a papain, mannyquee with hydrophobic properties. In addition, the blocking portion 30 may be formed by forming a self-assembled monolayer (SAM) to perform a hydrophobic treatment, and may be treated with a non-tilted series (CH3), silane treatment, or the like. It can be coated with Sigmacote (chlorinated organopolysiloxane in heptane), or can be treated with marking using PAP pen (proprietary component) or paraffin.

Further, the region surrounded by the projecting portion 14 can be subjected to a hydrophilic treatment. For example, the surface of the supporter 12 surrounded by the protrusion 14 is subjected to a plasma treatment using oxygen plasma or a corona discharge treatment, and the surface of the supporter 12 is negatively charged, . A self-assembled monolayer (SAM) can be formed on the surface of the support 12 in the region surrounded by the protrusion 14 to perform the hydrophobic treatment and the treatment with the carboxyl group system (COOH) or the like. It can be coated with Biolipidure 405® (Proprietary component). The blocking portion 30 of the region opened by the protrusion 14 on the surface of the support 12 is hydrophobic and the region surrounded by the protrusion 14 is selectively hydrophilic so that the portion 30 surrounded by the protrusion 14 It is possible to prevent the solution in the region from leaking.

Up to now, an exemplary embodiment of a single cell detection and collection slide used in an analytical apparatus to facilitate understanding of the present invention has been described and shown in the accompanying drawings. It should be understood, however, that such embodiments are merely illustrative of the present invention and not limiting thereof. And it is to be understood that the invention is not limited to the details shown and described. Since various other modifications may occur to those of ordinary skill in the art.

100: slide structure, 10: lower structure
12, 12a, 12b, 12c: Supports, 14, 14a, 14b, 14c:
16: detector, 18: analyzer
20: solution, 22a, 22b: object

Claims (13)

support fixture; And
And a protrusion protruded upward from the surface of the support,
Wherein the protruding portion has a shape in which at least one side is open,
Wherein the area enclosed by the protrusions can include a subject to be analyzed by the analyzer. The method according to claim 1,
The protrusion includes a first protrusion formed in a first direction; And
And a second protrusion formed in a second direction that is different from the first direction. 3. The method of claim 2,
And the protrusion includes a third protrusion formed in the same third direction as the first direction. The method of claim 3,
Wherein the first protrusion and the third protrusion are opposed to each other and facing each other. 3. The method of claim 2,
Wherein the second protrusion is formed in a curved shape. The method according to claim 1,
And a blocking portion formed on the support on at least one side of the protrusion. The method according to claim 6,
Wherein the blocking portion is relatively wettable relative to a surface area of the support enclosed by the protrusion. The method according to claim 6,
Wherein said blocking portion is a single cell detection and collection slide having hydrophobic properties. 9. The method of claim 8,
Wherein the blocking portion is formed of an organic solvent. 9. The method of claim 8,
The blocking portion may be formed by self-assembled monolayer (SAM) formation by a microchannel (CH3) or silane treatment, by coating with Sigmacote (chlorinated organopolysiloxane in heptane) or by using PAP pen (proprietary component) Single cell detection and collection slides treated with marking or paraffin. 9. The method of claim 8,
Wherein the surface area of the support enclosed by the protrusions is hydrophilic. 12. The method of claim 11,
Wherein the surface area of the support enclosed by the protrusions is a plasma treatment using oxygen plasma or a corona discharge treatment. 12. The method of claim 11,
The surface region of the support enclosed by the protrusions may include a self-assembled monolayer (SAM) formed by a carboxyl group (COOH) treatment or a single cell detection and collection slide coated with a Biolipidure 405® (Proprietary component) .

Images