KR20170087280A - Fat extraction and svf isolation kit - Google Patents

Fat extraction and svf isolation kit Download PDF

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Publication number
KR20170087280A
KR20170087280A KR1020160007049A KR20160007049A KR20170087280A KR 20170087280 A KR20170087280 A KR 20170087280A KR 1020160007049 A KR1020160007049 A KR 1020160007049A KR 20160007049 A KR20160007049 A KR 20160007049A KR 20170087280 A KR20170087280 A KR 20170087280A
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South Korea
Prior art keywords
fat
piston
support
body fat
stem cells
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KR1020160007049A
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Korean (ko)
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KR101782386B1 (en
Inventor
이홍기
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주식회사 이에이치엘바이오
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/50—Instruments, other than pincettes or toothpicks, for removing foreign bodies from the human body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/22—Implements for squeezing-off ulcers or the like on the inside of inner organs of the body; Implements for scraping-out cavities of body organs, e.g. bones; Calculus removers; Calculus smashing apparatus; Apparatus for removing obstructions in blood vessels, not otherwise provided for
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells

Abstract

The present invention relates to a fat collection and SVF separation kit, which comprises a collection means (101) for collecting a certain amount of body fat (A) by infiltrating the fat tissue to obtain adipose derived stem cells, and a collection means And separating means (102) for inducing a separation reaction between the substrate and the cells contained in the collected body fat (A) and filtering the lipid and the impurity (B) to obtain high purity stem cells (C) And to provide a simple and simplified fat collection and SVF separation kit which can carry out the separation of fat-derived stem cells together.

Figure P1020160007049

Description

[0001] FAT EXTRACTION AND SVF ISOLATION KIT [0002]

More particularly, the present invention relates to a kit for separating fat and SVF, and more particularly, to a kit for separating fat from fat tissue and separating lipids and impurities from stem cells, The present invention relates to a fat collection and SVF separation kit for easily obtaining and isolating adipose-derived stem cells.

The adipose tissue that is involved in the energy storage and metabolism of the body and occupies a large part of the human body includes stromal vascular fraction (SVF) including fibroblasts, microvascular endothelial cells and various immune cells in addition to adipocytes containing lipids. ). In particular, the fact that SVF contains stem cells has been reported, and related research is actively being done.

Stem cells are capable of differentiating into various human tissue cells under appropriate conditions under undifferentiated state, and have been applied to the field of cell therapy such as regenerating damaged tissue.

Among them, embryonic stem cells extracted from embryonic development are inevitably involved in ethical problems, and their research is limited. Especially, there is a possibility of inducing unnecessary cell proliferation in transplantation in vivo.

Therefore, it is effective to use adult stem cells extracted from already-grown body tissues such as bone marrow and fat for cell therapy by differentiating them into target organs to be treated.

However, adult stem cells extracted from bone marrow, umbilical cord blood, peripheral blood, nerve tissue, etc. are present in a small amount or cause pain upon harvesting.

In contrast, adult stem cells contained in adipose tissue have an advantage of being able to acquire abundant amounts with ease in harvesting and maintaining the characteristics and differentiation ability of stem cells at a level comparable to that of adult stem cells obtained from other sources, In particular, it has been shown that not only adipose tissue but also various tissues such as nerves and vascular tissues are differentiated, and the clinical application range of adipose derived adult stem cells is expected to be further expanded in the future.

In the meantime, the process of obtaining adipose-derived stem cells generally includes a process of collecting body fat from adipose tissue, a process of separating the extracellular matrix and SVF contained in the collected body fat, a centrifugation and a cell culture (Shaking incubation) To obtain stem cells.

The extraction of adipose tissue can be accomplished in a manner similar to liposuction as is commonly practiced in recent cosmetic procedures and is an example of a syringe-type liposuction device used in liposuction as disclosed in Published Japanese Patent Application No. 10-2009-0018295 A schematic configuration of a known liposuction device will be described below.

A piston inserted into the syringe and configured to suck and discharge liquid into and out of the syringe; a shield member formed detachably at the end of the syringe and having a through hole formed at the center thereof; A handle formed to be engaged with and separated from the piston by a detachment means and configured to be pulled or pushed by hand; a handle formed between the handle and the shield to pull the handle at all times, Locking means.

In addition, as another conventional technique similar to the above-described patent, the configuration of the liposuction syringe disclosed in Registration Utility Model No. 20-0376157 will be described.

A suction pipe having a small diameter to which a needle can be inserted can be formed at the center of the lower end surface and a predetermined size of the upper end opening of the suction pipe is inserted into the syringe through the upper end of the syringe, A plunger rod having a predetermined length at which a plurality of latching jaws are formed at regular intervals and a handle is formed at an upper end of the plunger rod, a piston which is slidably moved in close contact with the inner circumferential surface of the syringe cylinder in a state of being screwed to the lower end of the plunger rod, And a latching member for controlling the plunger rod so as to prevent the plunger rod from being pushed into the syringe barrel when the plunger rod is pulled out and pulled out in a state where the plunger rod is engaged with the upper end of the plunger rod.

Published Patent No. 10 - 2009 - 0018295 Registered Utility Model No. 20 - 0376157 - 0000 Registration No. 10 - 1202218 - 0000 Registration No. 10 - 0683280 - 0000

In the liposuction injector for a liposuction device for a liposuction device and a registration device of the prior art in which the above-described conventional techniques are applied, a syringe for liposuction is inserted into a syringe by pulling a plunger of the syringe, It is only for the purpose of collecting body fat simply by inhaling.

Therefore, in order to apply to the process of obtaining adipose stem cells from body fat, medical equipment such as a culture apparatus or a separation extractor must be provided for separating the extracellular matrix contained in body fat and SVF .

In addition, the above-described prior art has a difficulty in extracting a small amount of body fat due to a high sound pressure acting in the syringe, and the tissues other than the body fat are sucked in together, resulting in a side effect of rugged skin.

In order to prevent the piston or the plunger rod from being pushed into the syringe, the plunger rod is provided with a predetermined engaging member by applying a conventional locking syringe technology. However, in the process of pulling the plunger rod There is a disadvantage in that the operation process can not proceed smoothly due to the operation relationship in which the engaging jaw is repeatedly engaged in the groove.

Accordingly, the present invention has been made to solve the above-mentioned problems,

(101) that penetrates into adipose tissue to obtain adipose derived stem cells and collects a predetermined amount of body fat (A); and a body (101) that contains the body and cells contained in the body fat And separating means 102 for inducing a separation reaction and filtering lipids and impurities (B) to obtain high purity stem cells (C).

The harvesting means 101 includes a cylindrical body 110 having a hub 111 to which a body fat is charged at its tip and a first support 112 at a distal end,

A piston 120 having a second support table 121 installed at the rear end thereof for loading the body fat A into the body while forming a vacuum state by a backward operation while being inserted into the body 110,

A fixing tip 131 for forming a screw 133 on the inner circumferential surface so as to be attached to the hub 111 of the main body 110 and a cannula for collecting body fat through the suction port 132 formed at the tip of the tip 130,

The piston 120 is supported between the first support 112 and the second support 121 spaced apart from each other by a predetermined distance when the piston 120 moves backward to prevent the piston 120 from moving backward into the main body 110 And a lock bar (140).

In addition, the separating means 102 includes a separating tube 150 in the form of a cylindrical container for receiving the body fat A collected by the sampling means 101,

(B) layer and a stem cell (C) layer having a disk-like outer shape to be inserted into the separation tube (150) and having a mesh of several tens to several hundreds of micrometers ) Layer separator 170 for separating fat and stem cells from each other. The kit for isolating fat and isolating SVF can be provided.

The present invention is advantageous in that it is possible to conveniently perform a process of collecting fat and a process of separating stem cells to obtain adipose-derived stem cells.

Particularly, in the process of separating the SVF from the collected fat, it is possible to perform the collection and separation of the SVF in the operation room only by the practitioner's procedure without using the medical equipment such as the conventional incubator (shaking incubator) or the expensive separating extractor There is an advantage in that a sufficient amount of SVF can be extracted by using.

Accordingly, it is possible to reduce the economic burden due to the provision of separate expensive medical equipment or to reduce the economic burden due to the maintenance cost as in the prior art. In addition, since the cannula can be used for disposable use, It is possible to exclude infectious disease through the use of the drug, and it is easy to use.

In addition, the present invention is advantageous in that it can be easily improved by using a lock bar in a structure for preventing the piston from reversing due to a difference in air pressure during the process of collecting body fat, .

In addition, there is an advantage that the length of the lock bar can be variously applied to a tunneling operation to prevent a small amount of fat from being extracted or bending of the skin at the harvesting site.

1 is a perspective view of an embodiment of a fat collection and SVF separation kit of the present invention.
2 is an assembled state configuration diagram according to an embodiment of the fat collection and SVF separation kit of the present invention.
3 is an exploded view according to an embodiment of a fat collection and SVF separation kit of the present invention.
FIGS. 4 to 8 illustrate examples of the use of the fat collection and SVF separation kit according to the embodiment of the present invention. FIG.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the construction and operation of a fat collection and SVF separation kit according to a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 is a perspective view of an embodiment of a fat collection and SVF separation kit of the present invention, FIG. 2 is an assembly view of the fat collection and SVF separation kit of the present invention, FIG. FIGS. 4 to 8 together illustrate the use state of the SVF separation kit according to the embodiment of the present invention. FIG.

The fat collection and SVF separation kit 100 to which the technique of the present invention is applied collects body fat (A) from fat tissue that is capable of collecting adult stem cells most easily and in an abundant amount, (B) and stem cells (C) from a single kidney by simply providing a simple means of collecting and separating fat, which can easily perform a series of component separation processes, and collecting high-purity fat-derived stem cells And to isolate and obtain the desired product.

To this end, the fat collection and SVF separation kit 100 of the present invention comprises a collection means 100 for collecting a predetermined amount of body fat A by infiltrating the fat tissue to obtain adipose-derived stem cells as shown in FIGS. 1 to 3 (C) is obtained by inducing a separation reaction between a substrate and a cell contained in the body fat (A) collected by the sampling means (101) and filtering the lipid and the impurity (B) And a separating means (102) for separating the liquid.

The sampling means 101 includes a cylindrical body 110, a piston 120 for loading the body fat A into the body 110 while being inserted into the body 110, A cannula 130 mounted on a hub 111 of the body 110 to collect the body fat A and a lock bar 140 for preventing the piston 120 from being reversely moved back into the body 110 by a difference in air pressure .

The separating means 102 includes a separating tube 150 in the form of a cylindrical container for receiving the body fat A collected by the collecting means 101, And a cell separator 170 separating the body fat A into a lipid and impurity (B) layer and a stem cell (C) layer, comprising a mesh having a size of several tens to several hundreds of micrometers (탆) .

The body 110 is formed as a transparent cylindrical body similar to a conventional syringe. The body 111 has a hub 111 at its front end for loading the body fat A and a first support 112 at its distal end.

And a predetermined screw 114 for easy detachment of the cannula 130, which will be described later, is formed on the outer circumferential surface of the hub 111 so that the hub 111 can be mated.

The first support 112 is formed to have a predetermined width outside the main body 110 so that one side 141 of the lock bar 140 contacts with the first support 112. A predetermined step 113 is formed on the outer edge of the first support 112, So that the lock bar 140 is prevented from being detached in a state in which the lock bar 140 is mounted.

The piston 120 has a rod of a predetermined length with a packing at its tip. When the body 120 is inserted into the body 110, To the inside.

A second support 121 is formed at a rear end of the piston 120 so as to have a predetermined width so that the other side 142 of the lock bar 140 is in contact with the outer side of the piston 120. Similarly to the first support 112, So that the release of the lock bars 140 mounted between the first and second supports 112 and 121 is prevented.

The cannula 130 is a metal tube to be inserted into the body and has a fixing tip 131 for forming a screw 133 on the inner circumferential surface so as to be attached to the hub 111 of the main body 110 at its distal end, The body cavity A is drawn through the inlet 132 formed in the body cavity.

In addition, a predetermined hermetic means (not shown) may be further provided on the inner side of the fixing tip 131 so as to be more closely attached to the hub 111.

The lock bar 140 supports the first support 112 and the second support 121 spaced apart from each other by a predetermined distance when the piston 120 is retracted so that the piston 120 is moved toward the inside of the main body 110 Prevent backlash.

The piston 120 is retracted while the packing at the tip end is in tight contact with the inner diameter of the main body 110 and the body fat A is loaded through the hub 111. This is because the piston 120 This is due to the mechanism in which the pressure difference occurs. Means for preventing the body fat A charged in the main body 110 from flowing out is the reverse of the piston 120 toward the front side of the piston 120 where the low pressure is created in a state where the piston 120 is backward It is indispensable.

Conventionally, a locking type syringe is used to prevent the backward movement of the piston 120 as described above. However, since the syringe has a complicated structure such as a means for engaging the piston, there is a disadvantage that the usability is not smooth. So as to support between the first support 112 and the second support 121 so as to maintain the piston 120 in a backward state.

More specifically, the lock bars 140 are provided as a body having a triangular cross section at both side surfaces 141 and 142 which are in contact with the first and second supports 112 and 121, respectively, The first piston rod 144 is stably installed in the groove 122 formed in the rod of the piston 120 to support the first piston rod 112 and the second piston rod 121.

As described above, since the first and second supports 112 and 121 have the predetermined steps 113 and 123, the edge portion 144 is formed in the groove 122 formed in the longitudinal direction of the piston 120 rod, The bottom surface 143 of the lock bar 140 on which the lock bar 140 is seated can be prevented from being separated by the stepped portions 113 and 123.

In addition, in the preferred embodiment of the present invention, the length of the lock bars 140 is variously configured so that the lock bars 140a having a short length can be used when collecting a small amount of body fat A as in a tunneling procedure.

The separation tube 150 is configured in the form of a cylindrical container to receive the body fat A and insert the cell separator 170, which will be described later, inside the upper part.

The cell separator 170 separates the extracellular matrix such as residual lipid and impurity B through a mesh by treating the collected body fat A with a predetermined enzyme agent 160 and performing chemical decomposition, Increase the purity of the cell (C).

The enzyme agent 160 acts on the body fat A stored in the separation tube 150 to induce a separation reaction between the substrate and the cells. In other words, it is intended to induce an effective component separation reaction by chemically decomposing a cell mass contained in adipose tissue, and it is preferable to use a collagenase type. However, it is preferable to use a collagenase type such as Dispase, Accutase, Enzyme may be used.

Accordingly, the cell separator 170 has a mesh of a size of several tens to several hundreds of micrometers (mu m) inside the outer wall corresponding to the inner diameter of the separation tube 150, and the body fat A treated with the enzyme agent 160 is stored (B) layer and a stem cell (C) layer by inserting it into the interior of the separation tube (150).

Accordingly, the body fat A collected by the sampling means 101 is accommodated in the separation tube 150 and used for the component separation process.

Hereinafter, the process of separating the fat-derived stem cells (C) from the body fat (A) using the fat collection and SVF separation kit (100) having the above-described structure of the present invention will be described.

4, the screw 114 formed on the hub 111 of the main body 110 in which the piston 120 is inserted and the screw 133 formed on the fixing tip 131 of the cannula 130, And inserted from the acute portion of the cannula 130 into the adipose tissue of the human body.

5, when the piston 120 is gradually moved backward while the position of the main body 110 is fixed, the body fat A is charged into the main body 110 through the suction port 132 of the cannula 130 . The practitioner collects a desired amount while checking the scale of the main body 110.

When the collection of the body fat A is completed, the corner portions 144 are brought into contact with the piston rod 120 such that both end faces 141 and 142 of the lock bars 140 contact the first and second supports 112 and 121, So that the piston 120 is prevented from moving backward in the direction of the hub 111 of the main body 110 by the air pressure difference.

As described above, in order to perform the component separation process for obtaining the adipose-derived stem cells (C) when the process of collecting the body fat (A) is completed, the cell separator 170 is first attached to the separation tube 150 .

The cell separator 170 is inserted into the separator tube 150 at a predetermined depth so that the outer periphery of the separator tube 150 contacts the inner diameter of the separator tube 150 using a mesh having tens to several hundreds of micrometers (preferably, about 100 μm) The body fat A is stored in the separation tube 150 from the body 110 from which the body fat is removed.

7, since the extracellular matrix such as lipid and impurity (B) is contained in the body fat A and the adipocytes are present in the form of aggregated cell clusters, (150) and undergoes chemical decomposition.

8, the components contained in the body fat A are filtered by the cell separator 170, and the lipid and the impurities B and the stem cells are separated by different density differences, The SVF containing (C) is layered. Thus, the lipid and impurity (B) layer is completely removed to obtain high-purity fat-derived stem cells (C).

As described above, the fat collection and SVF separation kit 100 according to the present invention simplifies the structure for separating the body fat A and the stem cells C, And a single kit that can easily perform a series of processes based on the collection and separation of ingredients, thereby making it possible to carry out the procedure more stably.

100: fat collection and SVF separation kit 101: harvesting means
102: separating means 110:
111: hub 112: first support
113: step 114: screw
120: piston 121: second support
122: groove 123: step
130: Cannula 131: Fixing tip
132: inlet 133: screw
140: Rock bars 141, 142:
143: bottom surface 144:
150: Separation tube 160: Enzyme
170: cell separator A: body fat
B: Lipids and impurities C: Stem cells

Claims (3)

(101) for collecting and storing a predetermined amount of body fat (A) by infiltrating the fat tissue to obtain adipose-derived stem cells,
(102) for inducing a separation reaction between the substrate and the cells contained in the body fat (A) collected by the sampling means (101) and filtering the lipid and the impurity (B) to obtain high purity stem cells Wherein the SVF separation kit comprises: The method according to claim 1,
The sampling means (101)
A cylindrical body 110 having a hub 111 to which a body fat is to be loaded and a first support 112 at a distal end,
A piston 120 having a second support table 121 installed at the rear end thereof for loading the body fat A into the body while forming a vacuum state by a backward operation while being inserted into the body 110,
A fixing tip 131 for forming a screw 133 on the inner circumferential surface so as to be attached to the hub 111 of the main body 110 and a cannula for collecting body fat through the suction port 132 formed at the tip of the tip 130,
The piston 120 is supported between the first support 112 and the second support 121 spaced apart from each other by a predetermined distance when the piston 120 moves backward to prevent the piston 120 from moving backward into the main body 110 And a lock bar 140,
The lock bars 140 are formed in the shape of a triangle with both side surfaces 141 and 142 which are in contact with the first and second supports 112 and 121 and have corner portions 144 parallel to the bottom surface 143, Is configured to be stably installed in a groove (122) formed in a rod of the piston (120), so as to support between the first support (112) and the second support (121). The method according to claim 1,
The separating means (102)
A separation tube 150 in the form of a cylindrical container for receiving the body fat A collected by the sampling means 101,
(B) layer and a stem cell (C) layer having a disk-like outer shape to be inserted into the separation tube (150) and having a mesh of several tens to several hundreds of micrometers ) Layer separator (170). ≪ RTI ID = 0.0 > 17. < / RTI >
KR1020160007049A 2016-01-20 2016-01-20 Fat extraction and svf isolation kit KR101782386B1 (en)

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