KR20170082576A - Nutraceutical supplement with lactobacillus rhamnosus - Google Patents

Abstract

Compositions and their uses for reducing underlying symptoms of a variety of health problems, including arthritis, aging, and physical or athletic injuries, and for facilitating tissue healing and repair, may affect an individual's stem cells and inflammatory processes Lt; / RTI > and mixtures of natural plant chemicals.

Description

[0001] NUTRACEUTICAL SUPPLEMENT WITH LACTOBACILLUS RHAMNOSUS [0002]

 Technical field

The technology of the present invention is based on the finding that Lactobacillus The present invention relates to compositions and their uses for assisting joint support, aging, and sports medical recovery and performance, including lambsosus.

background

This section provides background information relating to the present application and is not necessarily prior art.

Treatment of a variety of musculoskeletal disorders and injuries is an ongoing problem. Structures that support the body's joints, ligaments, muscles, nerves, tendons, and limbs, neck, and back can be affected by degenerative diseases and inflammatory conditions that cause pain and impair normal activities. The body may also suffer from accidental injuries, including violent activity, repetitive activities, or abrasions, bruises, and fractures. Certain muscle skeletal problems arise from arthritis, aging, and participation in a variety of physical or athletic activities.

For example, arthritis is a common disease that causes inflammation that causes the joints and cartilage lining to deteriorate. Osteoarthritis is the most common form of arthritis. This is because aging "Wear and tear" and trauma to joints, such as sports injuries or fractures. The main symptoms of arthritic individuals include joint pain, swelling, warmth, weakness, weakness of joints, instability, catching, popping, stiffness, lack of sleep, muscle pain and fatigue . Autoimmune arthritis, such as rheumatoid arthritis, occurs when the body's immune system attacks itself. Osteoarthritis and rheumatoid arthritis are characterized by joint inflammation and cartilage degradation. Mesenchymal stem cells can reconstruct cartilage, where stem cells are present in the cartilage's superficial zone. Treatment of arthritis has relied on relieving symptoms by surgery, including exercise, straightening, weight loss, medication, and total arthroplasty. Agents, such as non-steroidal anti-inflammatory drugs (NSAIDs), have the risk of causing ulcers, heart attacks and kidney failure, including stomach, cardiovascular and kidneys. There is no treatment to improve osteoarthritis. Osteoarthritis is accelerated by increased joint response to obesity / weight gain.

Aging is a process of degeneration associated with the onset of many diseases. As people age, the prevalence of systemic inflammation-related conditions, such as obesity, increases, which is a sign of common aging. The body's ability to stimulate new bone marrow cells, including mesenchymal stem cells, diminishes during aging, resulting in weakened immune system and tissue regenerative capacity. Conditions that have been found to increase in prevalence according to current age and increased inflammation include osteoarthritis, rheumatoid arthritis, cardiovascular disease, diabetes, obesity, Alzheimer's disease, chronic kidney disease, autoimmune disease, cancer, and other diseases . Symptoms of aging are associated with underlying oxidative stress, increased inflammation, weakening of the immune system, and decreased ability of the body to form new cells and tissues. The main symptoms of aging include fatigue, drowsiness, changes in sleep patterns, poor memory, poor vision, wrinkles, poor dentition, sexual dysfunction, reduced sex drive, type 2 diabetes, increased weight gain, Constipation, skin changes including brown spots, loss of skin elasticity, menopause, hearing loss, increased bone fracture, arthritis.

Injuries resulting from physical and athletic activities include muscle contusion, joint sprains, ligament injuries, cartilage injuries, fractures and overuse conditions. Joints that orthopedists often treat as sports injuries include shoulders, knees, ankles, elbows, and wrists. Sports injuries can involve symptoms including pain, stiffness, swelling, unstable feelings, weakness, flushing, crepitation, bruising, and / or mechanical symptoms, such as locking or catching in the joints. Sports medicine has been developed professionally in the prevention and treatment of sport-related injuries from ligaments, muscles, tendons and bone. Conventional treatments for sports injuries include rest, ice, compression, and colossus. Other therapies include physical therapy and sports medicine rehabilitation, drugs, such as NSAIDs, to reduce inflammation that worsens injuries and causes surgery. There are no known drugs or existing treatments to accelerate the healing process after injury. Elderly athletes are at increased risk of sports injuries. Overweight obesity athletes are physically incompatible and increase the risk of injury, including arthritis, due to increased joint response.

Alleviation of one or more symptoms or problems resulting from joint problems, aging, and sports related injuries often involves the use of various anti-inflammatory treatments. Existing anti-inflammatory drugs show certain limitations. For example, the most commonly prescribed inflammatory / arthritic drugs are the various NSAID drugs. NSAIDs include generic medicines, such as ibuprofen, acetaminophen, naproxen, and aspirin, and also include prescription formulations of naproxen and celecoxib. Some of these drugs are associated with one or more side effects, some of which may be serious. Acetaminophen-related overdose is the reason for approximately 56,000 emergency room visits and about 26,000 hospitalizations and over 450 deaths annually, especially due to liver failure. Acetaminophen is also the first cause of acute liver failure and can lead to renal toxicity. NSAIDs can also induce nausea, heartburn, ingestion, abdominal pain, bleeding ulcers (GI symptoms), with approximately 16,500 people die each year as a result of NSAID-related gastrointestinal complications and an estimated 107,000 patients Are admitted each year with NSAID-related GI complications. NSAIDs may also provide complications related to heart attack, stroke (cardiovascular problems), congestive heart failure, atrial fibrillation, and renal damage.

It would be advantageous to have an alternative to NSAIDs for the treatment of a variety of health problems, including muscle skeletal disorders and injuries.

summary

In accordance with the present application, the techniques of the present invention include compositions and methods related to the treatment of various health problems, including muscle skeletal disorders and injuries.

In one embodiment, Lactobacillus < RTI ID = 0.0 > There is provided a dietary supplement comprising rhamnose , ginger, and vitamin D, wherein the dietary supplement can be administered to assist in health problems associated with any of joint support, aging, and sports medicine.

In another embodiment, the Lactobacillus < RTI ID = 0.0 > There is provided a dietary supplement comprising laminocus, ginger, vitamin D, curcumin, and Boswellia extract, wherein the dietary supplement provides for joint support and to alleviate one or more arthritis effects ≪ / RTI >

In yet another embodiment, the Lactobacillus < RTI ID = 0.0 > There is provided a dietary supplement comprising laminocus , ginger, vitamin D, boswellia extract, and green tea extract, wherein the dietary supplement may be administered to ameliorate one or more aging effects.

In yet another embodiment, the Lactobacillus < RTI ID = 0.0 > There is provided a dietary supplement comprising laminocus, ginger, vitamin D, curcumin, and Epimedium extract wherein the dietary supplement is administered to alleviate one or more effects resulting from body or athletic activities .

Further applications will become apparent from the detailed description of the present application. The description and specific examples of the present summary are for illustration purposes only and are not intended to limit the scope of the present invention.

details

The following description is merely exemplary in nature, manufacturing, and use of one or more of the inventions, and is not intended to limit the scope of the present invention to other applications or applications Application, or use of any specific invention claimed in the claims. In the disclosed methods, the order of steps provided is exemplary in nature, so that the order of the steps in the various embodiments may be different. All quantities herein are to be understood as being modified by the word "about" in describing the broadest scope of the art, unless explicitly stated otherwise.

It is to be understood that while the open-ended term "comprising " as a synonym for non-limiting terms, e.g., comprising, containing, or retaining is used in this application to describe and claim embodiments of the present technology, May be described using more restrictive terms, such as "consisting" or "consisting essentially of. &Quot; Thus, in any given embodiment that enumerates materials, components, or process steps, the teachings of the present invention also encompass such additional materials, components, or processes, even if not explicitly stated in the present application, Components, or steps (except in the case of a substantially constructed embodiment) except for the additional materials, components, or processes that affect the critical properties of the embodiment Or < / RTI > substantially constructed embodiments. For example, a description of a composition or process listing elements A, B, and C may be found in A, B, and C, except that specifically, And < RTI ID = 0.0 > C. ≪ / RTI > In addition, it should be understood that all natural supplements disclosed in this application may be provided and used in synthetic form within the scope of the present application.

All composition percentages referred to in this application are by weight of the total composition, unless otherwise specified. The disclosure of ranges includes all endpoints and subdivided ranges, including endpoints, unless otherwise specified. Thus, for example, ranges "A to B" or "about A to about B" The disclosure of numerical values and ranges of numerical values for certain parameters (e.g., amounts, weight percentages, etc.) does not exclude ranges of other numerical values and values that are useful in the present application. It is contemplated that two or more specific exemplary values for a given parameter may define endpoints for a range of values that may be recited in the claims with respect to that parameter. For example, if the parameter X is illustrated in the present application as having a numerical value A and is also illustrated as having the value Z, the parameter X is expected to have a range of values from about A to about Z. Similarly, the disclosure of ranges of two or more numerical values with respect to one parameter is intended to encompass the use of ranges of values relating to numerical values that may be recited in the claims using endpoints of the disclosed ranges (whether these ranges are nested, overlapping or otherwise) It is expected to cover all possible combinations. For example, if parameter X is exemplified as having values in the range 1-10, or 2-9, or 3-8 in the present application, the parameter X may also be 1-9, 1-8, 1-3 , 1-2, 2-10, 2-8, 2-3, 3-10, 3-9, and so on.

The technique of the present invention is based on the ability of the probiotic organism lactobacillus To a composition comprising rhamnose and to methods of administering such compositions to aid in various health problems, including joint support, aging, and sports medicine recovery and performance. In addition to Lactobacillus lambusosus, the composition may comprise a constituent selected from the group consisting of curcumin, boswellia extract, ginger, vitamin D, green tea extract, epimide extract, and combinations thereof. Curcumin can be provided by herb or its extract. In addition, it should be understood that curcumin can be provided and used in synthetic form within the scope of the present application. Boswell Ria extracts can be obtained from Boswell Ria other Serra (Boswellia serrata) (ie, Indian Franc Kin sense, Indian Frankincense). Ginger can be obtained from the roots of the serious diver operational during nalre (Zingiber officinale). Vitamin D may include cholecalciferol. Green tea extract can be obtained from Camellia sinen system (Camellia sinesnsis). The epimedium extract can be obtained from epimedium sagitatum (i.e., Sanko leaf extract). The compositions may be formulated for oral administration, including as tablets or capsules, in liquid or slurry form, or as powders or granules. Other excipients can be included including other ingredients such as, for example, one or more of anti-tack, binders, coatings, disintegrants, flavoring agents, coloring agents, lubricants, lubricants, sorbents, preservatives and sweeteners. Examples of particular excipients include one or more of hypromelose, rice flour, magnesium stearate, cellulose, inulin, and silicon dioxide.

Various embodiments may provide tailored compositions and uses thereof for particular musculoskeletal disorders and / or injuries. In one embodiment, a composition for mitigating the effects of one or more of arthritis provides joint support is Lactobacillus Laminocaine , vitamin D, curcumin, boswellia extract, and ginger. The composition can be used to alleviate arthritic symptoms and pain, stimulate the production of cartilage tissue from endogenous stem cells, and increase the body's antioxidant activity. In another embodiment, the composition for alleviating the aging effects comprises Lactobacillus laminocus , Bosswellia extract, Ginger, Green tea extract, and Vitamin D. This composition can be used to provide protective effects on cells and tissues against oxidative stress, reduce inflammation, and regulate the immune system. In yet another embodiment, a composition for alleviating problems arising from body and athletic activities comprises Lactobacillus lambosus, curcumin, ginger, vitamin D, and epimedium extract. This composition can be used to provide protection against oxidative stress, anti-inflammatory action, and immunomodulatory effect. The various constituents used in this composition are approved by the US Food and Drug Administration (FDA) for human use as food and dietary supplements and have side effects such as gastrointestinal irritation, gastric ulcers, hypertension, cardiovascular disease, It may appear limited or not at all.

Lactobacillus Lymphoscos is approved by the FDA for human use and can provide one or more activities that address various health problems, including muscle skeletal problems. Lactobacillus rhamnosus can reduce the inflammatory symptoms of rheumatoid arthritis in humans. Oral ingestion of Lactobacillus can improve the Health Assessment Questionnaire (HAQ) score and reduce levels of plasma inflammatory cytokines. Lactobacillus rhamnosus can improve arthritis in animals. Exopolysaccharide (EPS), a major component of Lactobacillus lambsosus , has anti-arthritis-inducing properties. EPS or EPS-generated probiotics can inhibit active arthritis. Lactobacillus lambosus can inhibit inflammation in animals. Lactobacillus lambosus can reduce the inflammatory signal in the immature field of animals. It can therefore be expected to inhibit inflammatory bowel syndrome, such as necrotizing enterocolitis (NEC), idiopathic inflammatory bowel disease (IBD), or infectious enteritis, in newborns or children. Lactobacillus laminocus can reduce GI and respiratory tract infections. Lactobacillus lambosus can reduce the risk for gastrointestinal and airway infections. Mice treated with Lactobacillus lambsosus showed a weakened weight gain in high fat diets (HD) (not normal diets [ND]) compared to mice treated with PBS (P). Mice that received Lactobacillus lambosus showed rapid glucose clearance (higher insulin sensitivity) than HD. Lactobacillus lambosus can protect human colon muscles. Lactobacillus lambsosus can weaken lipid polysaccharide (LPS) -induced inflammation that produces higher IL-6, lower IL-10 (anti-inflammatory) and can restore contractility of inflamed muscle tissue and cells have. Lactobacillus lambosus may increase insulin sensitivity. Lactobacillus lambusus can reduce the oxidative stress of athletes in intense exercise. Treatment with Lactobacillus rhamnosus can reduce plasma levels of active oxygen metabolites (dROM) and biological antioxidant capacity (BAP) in athletes with intense athletic training. Lactobacillus lambusosus can reduce the occurrence of UV-induced tumorigenesis. Hairless mice treated with Lactobacillus lambsos showed delayed tumor enlargement in both number and size. Lactobacillus lambusosus can enhance both congenital and acquired immunity. Treatment with Lactobacillus lambosus may increase plasma levels of peripheral leukocytes and peritoneal macrophages (phagocytic activity) and antibodies suggesting that Lactobacillus lambosus increases both congenital and acquired immunity .

Thus, Lactobacillus Lambosus offers several benefits including: Lactobacillus laminocus is a unique probiotic strain. Lactobacillus Lymphoscopy can weaken various types of arthritis. Lactobacillus Lymphocytes function as antioxidants that can make cartilage cells healthy. Lactobacillus Laminocus can reduce inflammatory cytokines that damage cartilage and joints. Lactobacillus Lymphoscopy can inhibit the expression of inflammatory genes that cause pain and stiffness associated with arthritis. Lactobacillus lambatus can improve rheumatoid arthritis. Lactobacillus lambatus can reduce oxidative stress and increase life span in the worm model, C. elegan. Lactobacillus lambosus can reduce inflammation. Lactobacillus lambosus can improve insulin-sensitivity and reduce fat accumulation. Lactobacillus laminocus can provide light protection against UV radiation. Lactobacillus lambosus shows anti-oxidative benefits in athletes in intense athletic training. Lactobacillus laminocus can reduce the risk of gastrointestinal and respiratory infections. Lactobacillus laminocus can reduce the incidence of diarrhea in high-fat diets. Lactobacillus laminocus can protect against UV-induced cancer formation. Lactobacillus lambatus is anti-inflammatory and immuno-regulated.

Lactobacillus Compositions comprising laminocus and their uses may be used in a variety of physical amounts of lactobacilli Ramno suspension and may have a Lactobacillus bar sealer's ramno suspension of varying amounts of colony forming units (CFU). Examples include physical amounts ranging from 1 mg to 1000 mg, 10 mg to 500 mg, 50 mg to 100 mg, and 67 mg. Examples also include CFU values in the range of 100 million to 1 trillion, 1 billion to 100 billion, 5 billion to 50 billion, and 10 billion. Other amounts of Lactobacillus lambusaceus may also be selected by the skilled artisan in the field as needed. Such quantities may be provided, for example, as a composition comprising a single dose or as multiple doses and administered as a single capsule or as a plurality of capsules and may be mixed with other components.

Curcumin is approved by the FDA for human use and can provide one or more activities that address a variety of health problems, including muscle skeletal problems. As mentioned, curcumin can be provided by herbal extracts or herbal extracts. In addition, it should be understood that curcumin can be provided and used in synthetic form within the scope of the present application. Curcumin can have the same analgesic effect as ibuprofen in patients with osteoarthritis. Curcumin can be as effective as ibuprofen for the treatment of osteoarthritis of the knee, but has fewer side effects than ibuprofen. Curcumin can inhibit the activity of human lubricating membrane cells that cause rheumatoid arthritis. Curcumin can inhibit the production of inflammatory cytokines, which cause rheumatoid arthritis in patients, from synovial cells. It can also inhibit the expansion of human synovial cells. Curcumin can inhibit inflammation and arthritis in animals. Curcumin can inhibit collagen-induced arthritis (CIA) as well as the production of inflammatory cytokines in animals. Curcumin can augment muscle regeneration after traumatic injury. Systemic treatment with curcumin can increase muscle mass (embryonic myosin heavy chain [EMHC]) and muscle cells in two different areas of the injured muscle (leg & tibial muscle [TA]). Curcumin can increase cardiac muscle repair and improve heart failure. Curcumin can inhibit the production of malononaldehyde (MDA [A]), lipid peroxides, and matrix metalloproteases (MMPs) that damage cardiac muscle in animal models of myocardial infarction. Curcumin can inhibit oxidative stress that causes muscle damage. Curcumin can inhibit the production of oxides (hydrogen peroxide & NADPH-oxidase) and muscle damage markers (MCP-1 & CXCL14) in muscles of animals that have performed quite a lot of exercise.

Thus, curcumin provides several advantages including: Curcumin can be as safe and effective as NSAID in the treatment of knee osteoarthritis. Curcumin can be anti-inflammatory. Curcumin can reduce inflammatory cytokines in cartilage. Curcumin can show anti-arthritic effects in animals. Curcumin can promote cartilage formation from mesenchymal stem cells. Curcumin can reduce inflammation in the submucosal layer of the human joint. Curcumin can inhibit collagen-induced arthritis in animals. Curcumin can have a therapeutic effect in the treatment of osteoarthritis. Curcumin can promote muscle recovery. Curcumin can prevent loss of muscle mass after traumatic injury and stimulate muscle regeneration. Curcumin can reduce inflammation and improve odd exercise-induced muscle damage. Curcumin can promote heart recovery. Curcumine can reduce oxidative stress after sloping running-induced muscle damage. Curcumin can have a therapeutic effect on the treatment of osteoarthritis.

The composition comprising curcumin and its uses may utilize various amounts. Examples include amounts ranging from 1 mg to 1000 mg, 10 mg to 700 mg, 100 mg to 500 mg, and 350 mg. Other curcumin quantities may also be selected by skilled artisans in the field as needed. Such quantities may be provided, for example, as a composition comprising a single dose or as multiple doses and administered as a single capsule or as a plurality of capsules and may be mixed with other components.

Curcumin is approved by the FDA for human use and can provide one or more activities that address a variety of health problems, including muscle skeletal problems. As mentioned, the boswellia extract may comprise boswellia ceratta extract. Bosswellia extracts can reduce osteoarthritis symptoms. Patients with osteoarthritis who received botulinum plant extracts reported knee pain and decreased swelling, increased knee motion, and increased walking distance. Bosswellia botanical extracts reduced osteoarthritis-related pain and bodily functions (visual pain scale), recurrence function index, and WOMAC pain and stiffness. Bosswellia extracts can reduce arthritis and inflammation in animals. Bosswellia extracts may reduce arthritis scores, plantar edema, and local tissue pre-inflammatory cytokine tumor necrosis factor alpha (TNF-a) and interleukin-1 beta (IL-1 beta) in Lewis rat adjuvant arthritis animal models . Bosswellia extract can restore memory in animals. Orally ingested boswellia extracts may reduce escape latency and travel distance, but have no effect on swim speed in the Morris water maze, suggesting that it enhances spatial memory in animals. Bosswellia extracts may have anti-tumor and anti-hyperlipidemic effects in animals. Oral ingested boswellia plant extracts may reduce TPA-induced skin inflammation and edema formation.

Thus, the boswellia extract provides several advantages including: Bosswellia extract significantly improves pain scores and physical function in patients with osteoarthritis. Bosswellia extract can inhibit the activation of the inflammatory immune system. Bosswellia extract can block cartilage matrix breakdown and increase type 2 collagen and aggrecan in human osteoarthritic cartilage cells. Bosswellia extract can reduce cartilage-degrading enzymes and inflammation in osteoarthritis patients. Bosswellia extracts may be anti-inflammatory, anti-arthritic, and analgesic. Bosswellia extracts can reduce knee pain and increase knee motion and walking distance. Bosswellia extract may reduce joint inflammation and spondylarthritis. Bosswellia extract can improve memory in aging animal models. Bosswellia extracts may have anti-oxidant and anti-thrombotic effects. Bosswellia extract can increase the elasticity of light-aging skin. Bosswellia extract can exert anti-tumor and anti-hyperlipidemic effects.

Compositions comprising boswellia extracts and their uses may have varying amounts of boswellia extract and varying amounts of boswellelic acid. Examples include a physical amount in the range of 1 mg to 1000 mg, 10 mg to 800 mg, 100 mg to 600 mg, and 500 mg. In addition, it should be understood that the boswellia extract may be provided and used in synthetic form within the scope of the present application. Other amounts of other boswellia extracts may also be selected by the skilled artisan in the field as needed. Examples also include various boswellic acid percentages, including physical quantities of 1% to 100%, 10% to 90%, 25% to 75%, and 65%. These physical amounts and percentages may be provided and administered as a composition comprising, for example, a single dose or as multi-dose dosages, provided as a single capsule or as multiple capsules and mixed with other components .

Ginger is approved by the FDA for human use and can provide one or more activities to address a variety of health problems, including muscle skeletal problems. As mentioned, the ginger can be obtained from the root of the displacement member during the operational nalre (Zingiber officinale). Ginger may be as effective as NSAIDs in reducing arthritis pain without gastric disease in patients. Ginger-containing supplements (Zinaxin) reduced arthritis pain (visual pain scale [VAS]) to the same degree as diclofenac, NSAID. Furthermore, Zinaxin does not cause gastric diseases accompanied by NSAIDs. Ginger can alleviate symptoms of osteoarthritis. Patients with gingival osteoarthritis showed no signs of any negative effects and showed significant improvement in osteoarthritis symptoms (based on the Health Assessment Questionnaire) and gradual improvement. Ginger can reduce inflammation and muscle pain caused by exercise in patients. In 98 patients treated with ginger for 6 weeks, there was a significant reduction in muscle pain and inflammation.

Thus, ginger provides several advantages including: Ginger therapy can exhibit remarkable relief of osteoarthritis symptoms with almost no side effects. Ginger can inhibit joint inflammation by reducing inflammatory cytokines in rheumatoid arthritis. Ginger may be as effective as betamethasone, a potent steroid, in reducing osteoarthritis and rheumatoid arthritis. Ginger can reduce arthritis pain in patients by as much as diclofenac (NSAID). Ginger can have antioxidant and protective effects against acetaminophen in animals. Ginger may have anti-oxidative, anti-inflammatory, anti-cancer, and anti-diabetic effects. Ginger can reduce muscle pain caused by exercise. Ginger can reduce muscle pain caused by exercise. Long - term treatment with ginger can relieve symptoms of osteoarthritis with few side effects.

Compositions comprising ginger and their uses may have a variety of physical quantities of ginger wherein the physical amount may include various concentration ratios. Examples include a physical amount in the range of 1 mg to 1000 mg, 10 mg to 800 mg, 100 mg to 600 mg, and 500 mg. Examples also relate to a range of ginger concentrations, including physical amounts ranging from 1.5: 1 to 20: 1, 2: 1 to 15: 1, 5: 1 to 10: 1, and 8: Equivalent concentrations of gingerol (active ingredients of ginger) may also be used. In addition, it should be understood that ginger may be provided and used in synthetic form within the scope of the present application. Other amounts of ginger may also be selected by the skilled artisan in the field as needed. Such quantities may be provided, for example, as a composition comprising a single dose or as multiple doses and administered as a single capsule or as a plurality of capsules and may be mixed with other components.

Vitamin D is approved by the FDA for human use and can provide one or more activities that address a variety of health problems, including muscle skeletal problems. As mentioned, vitamin D may include cholecalciferol. Vitamin D can reduce the effects of osteoarthritis in humans. Daylight exposure and serum vitamin D (25 [OH] D) levels are positively correlated with decreased knee cartilage loss. Thus, implementing vitamin D sufficiency can prevent and / or delay cartilage loss in osteoarthritis of the knee. Vitamin D (1,25 (OH) 2D3) can reduce arthritis production of matrix metalloprotease-9 and prostaglandin E2 (PGE2) in human articular cartilage cells. Vitamin D may increase adequate bone formation and may reduce unwanted aortic calcification in high phosphate diet. In chronic kidney disease, patients lose the production of appropriate bone-forming factors and thus increase the unnecessary aortic calcification. Paricalsitol, which contains vitamin D, can reverse all of these. Vitamin D can increase bone formation. Vitamin D (1,25 (OH) 2D3) can increase the expression of protein factors (type 1 collagen, osteopontin, sialoprotein, osteocalcin, alkaline phosphatase, and BMP-2) have.

Thus, vitamin D provides several benefits including: Vitamin D levels are positively associated with improved knee cartilage volume. Vitamin D can inhibit inflammation in osteoarthritis and rheumatoid arthritis. Vitamin D can exert an anti-inflammatory action on the submucosal layer cells. Vitamin D levels can be associated with bone mineral density and cartilage protection in osteoarthritis knees. Vitamin D-deficient athletes may have smaller heart sizes. Vitamin D deficiency can be associated with a lower body mass of professional soccer players. Vitamin D directly affects skeletal muscle structure and function. Vitamin D can prevent overuse-induced injuries, such as fatigue fractures. Vitamin D can modulate the age-related decline of muscle function, which benefits older athletes. Vitamin D is used for DNA repair and can exert an anti-aging effect. Vitamin D can have protective effects on cardiovascular disease, diabetes, autoimmune diseases and cancer. Vitamin D may be important for anti-aging of bone. Vitamin D can increase the expression of anti-aging genes. Vitamin D may include bone formation in mesenchymal stem cells.

Compositions comprising vitamin D and their uses may have varying amounts of vitamin D, including a wide range of international units (IU). Examples include IU values ranging from 1 IU to 10,000 IU, 10 IU to 7,500 IU, 100 IU to 5,000 IU, 500 IU to 2,000 IU, and 1,000 IU. In addition, it should be understood that vitamin D may be provided and used in synthetic form within the scope of the present application. Other amounts of vitamin D may also be selected by the skilled artisan in the field as needed. Such quantities may be provided, for example, as a composition comprising a single dose or as multiple doses and administered as a single capsule or as a plurality of capsules and may be mixed with other components.

Green tea extracts are approved by the FDA for human use and can provide one or more activities to address a variety of health problems, including muscle skeletal problems. As mentioned, green tea extracts can be obtained from Camellia sinensis and can contain a variety of weight percentages of polyphenols, catechins, and epigallocatechin gallates (EGCG). Green tea extract can inhibit skin damage and aging induced by UV light. Cream containing green tea extract may reduce UV-induced light-aging and subsequent immunosuppression. Green tea extracts can increase learning and memory in older rats. Both young and old rats treated with green tea extract find shorter periods of time to find a safe place (closed [dark] space, closed [dark] arm) and take appropriate avoidance actions for pain-inducing conditions gave. Green tea extracts may have bone-forming and anti-fat effects. Green tea extracts can increase the activity of bone-forming alkaline phosphatase (ALP) and inhibit the proliferation of fat cells (fat cells, adipocytes).

Thus, the green tea extract provides several advantages including the following. Green tea extracts may be effective in enhancing learning and memory. Green tea extracts may have bone-forming and anti-obesity effects. Green tea extracts may have anti-oxidation and anti-aging effects. Green tea extracts can have a UV protection effect. Green tea extracts can protect blood cells from aging-induced oxidative stress.

Compositions comprising green tea extracts and their uses may have various physical amounts of green tea extract and varying amounts of polyphenols, catechins, and epigallocatechin gallates. Examples include a physical amount in the range of 1 mg to 1000 mg, 10 mg to 800 mg, 100 mg to 600 mg, and 500 mg. Examples also include polyphenols with a physical amount of 1% to 100%, 10% to 100%, 50% to 100%, and 98%. Examples also include those wherein the physical amount comprises 1% to 100%, 10% to 100%, 50% to 100%, and 80% catechins. Examples also include epigallocatechin gallate with a physical amount of 1% to 100%, 10% to 90%, 25% to 75%, and 50%. In addition, it is to be understood that the green tea extract may be provided and used in synthetic form within the scope of the present application. Other amounts of other green tea extracts may also be selected by the skilled artisan in the field as needed. Such quantities may be provided, for example, as a composition comprising a single dose or as multiple doses and administered as a single capsule or as a plurality of capsules and may be mixed with other components.

Epimedium extracts are approved by the FDA for human use and can provide one or more activities to address a variety of health problems, including muscle skeletal problems. The epimedium extract as mentioned can be obtained from epimedium sagitatum (i.e., Sanshipu mushroom extract) and may have various weight percentages of the iscarin . Epimedium extracts can increase the formation of bone cells. Treatment with epimedium extract increased the formation of bone cells from mesenchymal stem cells. Epimedium extract has a vaso-relaxing effect in animals. Icarine, a major component of epimedium extract, can reduce blood pressure by relaxing the coronary arteries and increasing the activity of antioxidant enzymes and eNOS in blood vessels. Epimedium extracts can enhance peripheral nerve regeneration. The epimeric extract (Icarin: major component) can promote peripheral nerve regeneration and improve the function of damaged nerves in crush injury animal models. Epimedium extracts can mimic one or more effects of testosterone. Icarine (a major component of the ICA-epimedium extract) can increase the level of serum bone Gla-protein (BGP) and serum testosterone circulation, which are markers of bone growth.

Thus, Epimedium Extract provides several advantages including the following. The epimedium extract may be a testosterone-mimetic. Epimedium extracts can enhance the activity of anti-oxidants, eNOS and NO. Epimedium extracts can enhance the formation of bone cells from mesenchymal stem cells. Epimedium extract can reduce bone resorption by improving bone formation. Epimedium extracts may have cardiovascular therapeutic effects. Epimedium extract can restore peripheral nerve regeneration by restoring damaged nerve function.

Compositions comprising epimeric extracts and their uses may have various physical amounts of epimediamine extract having various weight percentages of the iscarinic. Examples include a physical amount in the range of 1 mg to 1000 mg, 10 mg to 800 mg, 100 mg to 600 mg, and 500 mg. Examples also include various weight percent iscarins, including 0.1% to 100%, 1% to 90%, 1% to 50%, 5% to 20%, and 10% physical quantities. In addition, it should be understood that the epimedium extract may be provided and used in synthetic form within the scope of the present application. Other amounts of other epimediate extracts may also be selected by the skilled artisan in the field as needed. Such quantities may be provided, for example, as a composition comprising a single dose or as multiple doses and administered as a single capsule or as a plurality of capsules and may be mixed with other components.

The compositions can typically be formulated in a variety of ways for oral administration. Examples include formulating the composition into various tablets or capsules, providing the composition in liquid or slurry form, or providing the composition as a powder or granules. The composition components may each be provided as separate parts and may be mixed together as a single part, or the various components may be mixed, wherein the total composition is provided by one or more parts, . Other dosage forms suitable for oral administration may be used.

(For example, magnesium stearate), a binder (e.g., saccharide, gelatin, a polymer), a coating agent (e.g., hydroxypropyl methylcellulose, a dressing agent such as wax, a plasticizer, a fiber, etc.) (For example, talc, silica, fat), a lubricant (e.g., fumed silica, talc, magnesium carbonate), a hygroscopic agent, a lubricant such as polyvinylpyrrolidone, carboxymethylcellulose, modified starch, Various excipients may be included in the compositions of the present invention, including preservatives (e.g., antioxidants such as vitamins A, E, and C), and sweeteners. Examples of particular excipients include one or more of hypromelose, rice flour, magnesium stearate, cellulose, inulin, and silicon dioxide.

The compositions of the invention and methods of administering such compositions can affect how the body's stem cells respond to a variety of health problems, including muscle skeletal problems. Stem cells contain distinct types of cells with notable potential for developing many different cell types. Stem cells can differentiate into other cell types, including fat, muscle, bone, cartilage, nerves, and blood vessels. These are important during their youthful growth and in restoring / supplementing their organizations. When stem cells develop into specific cells, such as adipocytes, they typically can not form other types of tissues, such as bone, cartilage, and muscle. The composition of the present invention does not affect stem cells, thereby reducing the inflammatory process and improving essential symptoms. Keeping the tissues healthy can also improve disease and reduce symptoms / progress in healthy conditions.

Cells and tissues can be damaged by stress, sleep deprivation, travel, certain drugs, smoking, injury, aging, poor diet, toxins, autoimmune diseases and many other causes. Stem cells may be involved in the healing and repair of these damaged cells and tissues. Stem cells provide a renewable source of cells and tissues for treating a number of diseases and conditions, including arthritis, aging, and sports medicine injuries. Damaged cells and tissues can lead to accelerated injury, including aging and sports injuries. Currently, obesity is a pandemic that results in stem cells forming more fat, resulting in defects of bone, cartilage and blood vessels, leading to diabetes, hypertension, osteoporosis, arthritis, accelerated aging and sports medicine injuries .

The compositions of the present invention and their uses entail a paradigm shift in terms of dealing with the aging process. In order to slow the aging process, it is believed that it is important to maintain the health of cells that supplement tissue (e.g., mesenchymal and hematopoietic stem cells). Aging reduces the number of stem cells. Numerous scientific studies indicate that individual supplements can protect these cells in the compositions of the present invention. However, the combinations used in the present compositions revolutionize current thinking and approach to support joint health, anti-aging, and sports medicine performance / recovery. For example, without being limited to any particular theory, the compositions of the present invention and methods of using such compositions may allow one's own stem cells to directly form excess fat muscle, bone, cartilage, nerves, and blood vessels I think. In research studies, individual supplements of the compositions of the present invention have shown a significant improvement in the ability to downregulate inflammatory chemicals and genes that are responsible for the disease process. These effects have been found through extensive research including clinical studies involving patients with various conditions. In the compositions of the present invention, the individual components have shown the ability to protect cells and promote cartilage differentiation of mesenchymal stem cells (MSCs).

Example

One specific example of a composition that provides joint support and alleviates one or more arthritic effects includes Lactobacillus Laminocaine , vitamin D, curcumin, boswellia extract, and ginger. The composition is formulated as a capsule and the serving size is two capsules which contain ingredients or ingredients as shown in Table 1 below.

 Constituent  Amount per serving %  Daily figure Vitamin D3 (as cholecalciferol) 1000 IU 250% Ginger root extract 500 mg n / a Bosswellia Serratapae extract 500 mg n / a Curculum as Root Extract 350 mg n / a Lactobacillus lambosus 10 billion CFU n / a

n / a = Daily number is not determined.

Other Ingredients: Hypromellose (capsules), rice flour, magnesium stearate.

Adipogenesis testing with the composition of Table 1:

Differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) into adipocytes.

Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA). After thawing mononuclear cells were incubated with α-minimal essential medium (α-MEM, Invitrogen, Carlsbad, CA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Invitrogen, Carlsbad, Calif.) And 1% antibiotics and antifungal , Carlsbad CA). The cells were plated at 1-5 x10 ⁶ cell density per 100 cm ² dish. The culture was maintained in a 5% CO ₂ incubator at 37 ° C and the medium was changed after 48 hours and every 3-4 days thereafter. When the MSCs were dense, cells were harvested by addition of 0.25% trypsin / EDTA (Invitrogen, Carlsbad, Calif.). MSCs (2-3 passages) were plated in 75-cm ² flasks or 24-well plates and cultured in a-MEM with 20% FBS to a density of up to 2.0 x 10 ⁴ cells / cm ² . This medium was replaced with fat-producing medium, and cells were cultured for an additional 14 days. This fat-producing medium contained the COX-1 inhibitor (2-valeryloxybenzoic acid, Cayman, Ann Arbor MI) and the COX-2 inhibitor (3- (4- methylsulfonylphenyl) -4-phenyl- 20-HETE agonist (20-5,14-HEDE) or 20-OH-PGE ₂ and in conjunction with 20-HETE, 20-OH-PGE ₂ and in the presence or absence of DMEM-high glucose , Complete culture medium supplemented with 10% (v / v) FBS, 10 μg / ml insulin, 0.5 mM dexamethasone (Sigma-Aldrich, St. Louis, Mo.), and 1% antibiotic and antifungal agent (Invitrogen, Carlsbad, CA) . 20-HETE, 20-HETE agonists or 20-OH-PGE ₂ were added three times per week at 0.1 and 1 μM concentrations. The inhibitors of COX-1 and COX-2 were added three times per week in a volume of 100 [mu] M and 5 [mu] M, respectively.

Oil Red O dyeing.

On the 14th day of fat production, 0.21% Oil Red O (Sigma-Aldrich, St. Louis, MO) in 100% isopropanol was used. In summary, adipocytes were fixed in 10% formaldehyde, washed in Oil-red O for 10 min, rinsed in 60% isopropanol (Sigma-Aldrich, St. Louis, Mo.) and oil red O was added with 100% isopropanol , Eluted for 10 minutes and read at 490 nm for 0.5 seconds to measure the OD. MSC-derived adipocytes were measured with Oil red O staining (OD = 490 nm) after 14 days. Each Oil red O staining value was normalized to cell number (OD = 490 nm).

In the test, it was observed that the formulation of Table 1 in the Oil Red O staining test resulted in a statistically significant decrease in fat production compared to untreated human MSC. In addition, the test showed that, in the absence of Lactobacillus lambusosus , there was no statistically significant reduction in fat production despite the addition of other ingredients in the formulation of Table 1. The addition of Lactobacillus rhamnosus is thought to be a cause of synergy in combination with other ingredients in reducing fat production.

Anti-oxidative stress.

Percentages of live and dead cells were determined simultaneously by measuring intracellular esterase activity and plasma membrane integrity using the LIVE / DEAD® Viability / Cytotoxicity Assay Kit (Life Technologies). In summary, human MSCs were plated on 24-well plates. The next day, cells were treated with the components and 100 μM H ₂ O ₂ for 12 hours. Supernatant medium from each well was collected in microtubes, the cells were washed with 1X PBS, and the washings were collected in microtubes, respectively, to collect any floating or dead cells. Cells attached at the bottom of plate were incubated in 1X PBS in a 37 ° C incubator. The microtube was centrifuged at 2000 rpm for 3 minutes to obtain cell pellet. After removal of the supernatant, the cell pellet was resuspended in 1X PBS containing 2 [mu] M calcerin AM and 4 [mu] M ethidium / homodimer. The re-suspended cells were again poured into each well and the plates were incubated at 37 [deg.] C for 15 minutes. The cells were then photographed using a fluorescence microscope (Olympus IX71) at 10X magnification.

The anti-oxidative stress test showed that the other ingredients of the formulation of Table 1, when tested alone, did not show a significant improvement in cell viability after 12 hours exposure to H ₂ O ₂ . However, the preparations in Table 1 resulted in a noticeable improvement in cell viability after 12 hours exposure to H ₂ O ₂ . These test results further support the idea that the combination of the above components has a synergistic effect in protecting cells against oxidative stress and ultimate apoptosis.

It is also believed that the formulations of Table 1 will have beneficial effects on cartilage and osteogenic differentiation of human MSCs. Inflammation is detrimental to cartilage formation and cartilage cell formation from human MSCs. The formulation of Table 1 shows an anti-inflammatory effect as implied by a significant reduction in fat production in the experiments described above.

One specific example of a composition for alleviating the aging effect is lactobacillus Lambsrus , boswellia extract, ginger, green tea extract, and vitamin D. The composition is formulated as a capsule, the serving size is two capsules, which contain ingredients or constituents as shown in Table 2 below.

 Constituent Amount per serving %  Daily figure Vitamin D (as cholecalciferol) 1000 IU 250% Green Tea (Leaf) Extract (Camellia sinen system, Camellia sinensis)
(Standardized for 98% polyphenol, 80% catechin, and 50% EGCG) 500 mg n / a Bosswellia sera extract
(Standardized for 65% boswellel acid) 500 mg n / a Ginger roots (from 8: 1 concentrate) ( genitalia ) 500 mg n / a Lactobacillus lambosus 10 billion CFU n / a

n / a = Daily number is not determined.

Other ingredients: Cellulose (capsule), inulin, silicon dioxide, vegetable magnesium stearate.

Fat Production Test Using the Composition of Table 2:

Differentiation of human bone marrow-derived MSC into adipocytes.

Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA). After thawing mononuclear cells were incubated with α-minimal essential medium (α-MEM, Invitrogen, Carlsbad, CA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Invitrogen, Carlsbad, Calif.) And 1% antibiotics and antifungal , Carlsbad CA). The cells were plated at 1-5 x10 ⁶ cell density per 100 cm ² dish. The culture was maintained in a 5% CO ₂ incubator at 37 ° C and the medium was changed after 48 hours and every 3-4 days thereafter. When the MSCs were dense, cells were harvested by addition of 0.25% trypsin / EDTA (Invitrogen, Carlsbad, Calif.). MSCs (2-3 passages) were plated in 75-cm ² flasks or 24-well plates and cultured in a-MEM with 20% FBS to a density of up to 2.0 x 10 ⁴ cells / cm ² . This medium was replaced with fat-producing medium, and cells were cultured for an additional 14 days. This fat-producing medium contained the COX-1 inhibitor (2-valeryloxybenzoic acid, Cayman, Ann Arbor MI) and the COX-2 inhibitor (3- (4- methylsulfonylphenyl) -4-phenyl- 20-HETE agonist (20-5,14-HEDE) or 20-OH-PGE ₂ and in conjunction with 20-HETE, 20-OH-PGE ₂ and in the presence or absence of DMEM-high glucose , Complete culture medium supplemented with 10% (v / v) FBS, 10 μg / ml insulin, 0.5 mM dexamethasone (Sigma-Aldrich, St. Louis, Mo.), and 1% antibiotic and antifungal agent (Invitrogen, Carlsbad, CA) . 20-HETE, 20-HETE agonist or 20-OH-PGE ₂ were added at a concentration of 0.1 and 1 μM three times per week. The inhibitors of COX-1 and COX-2 were added three times per week in a volume of 100 [mu] M and 5 [mu] M, respectively.

Oil Red O dyeing.

On the 14th day of fat production, 0.21% Oil Red O (Sigma-Aldrich, St. Louis, MO) in 100% isopropanol was used. In summary, adipocytes were fixed in 10% formaldehyde, washed in Oil-red O for 10 min, rinsed in 60% isopropanol (Sigma-Aldrich, St. Louis, Mo.) and oil red O was added with 100% isopropanol , Eluted for 10 minutes and read at 490 nm for 0.5 seconds to measure the OD. MSC-derived adipocytes were measured with Oil red O staining (OD = 490 nm) after 14 days. Each Oil red O staining value was normalized to cell number (OD = 490 nm).

In the test, it was observed that the formulation of Table 2 in the Oil Red O staining test resulted in a statistically significant reduction in fat production compared to untreated human MSC. In addition, the test showed that, in the absence of Lactobacillus lambusosus, the addition of other ingredients in the formulation of Table 2 did not result in any statistically significant reduction in fat production. The addition of Lactobacillus rhamnosus is thought to be a cause of synergy in combination with other ingredients in reducing fat production.

Anti-oxidative stress.

Percentages of live and dead cells were determined simultaneously by measuring intracellular esterase activity and plasma membrane integrity using the LIVE / DEAD® Viability / Cytotoxicity Assay Kit (Life Technologies). In summary, human MSCs were plated on 24-well plates. The next day, cells were treated with the components and 100 μM H ₂ O ₂ for 12 hours. Supernatant medium from each well was collected in microtubes, the cells were washed with 1X PBS, and the washings were collected in microtubes, respectively, to collect any floating or dead cells. Cells attached at the bottom of plate were incubated in 1X PBS in a 37 ° C incubator. The microtube was centrifuged at 2000 rpm for 3 minutes to obtain cell pellet. After removal of the supernatant, the cell pellet was resuspended in 1X PBS containing 2 [mu] M calcerin AM and 4 [mu] M ethidium / homodimer. The re-suspended cells were again poured into each well and the plates were incubated at 37 [deg.] C for 15 minutes. The cells were then photographed using a fluorescence microscope (Olympus IX71) at 10X magnification.

The anti-oxidative stress test showed that the other ingredients of the formulation of Table 2, when tested alone, did not show a significant improvement in cell viability after 12 hours exposure to H ₂ O ₂ . However, the preparations in Table 2 resulted in a noticeable improvement in cell viability after 12 hours exposure to H ₂ O ₂ . These test results further support the idea that the combination of the above components has a synergistic effect in protecting cells against oxidative stress and ultimate apoptosis.

It is also believed that the formulations of Table 2 will have beneficial effects on cartilage and osteogenic differentiation of human MSCs. Inflammation is detrimental to cartilage formation and cartilage cell formation from human MSCs. The formulation of Table 2 shows the anti-inflammatory effect as implied by the marked reduction in fat production in the experiments described above.

One example of a composition for alleviating problems arising from body and athletic activities is lactose Ramno include suspension, curcumin, ginger, vitamin D, and epi medium extract. The composition is formulated as a capsule and the serving size is two capsules which contain ingredients or ingredients as shown in Table 3 below.

 Constituent Amount per serving %  Daily figure Vitamin D (as cholecalciferol) 1000 IU 250% Curcumin 350 mg n / a Epimemic Sagittumu
(Standardized for 10% iscrin) 500 mg n / a Ginger roots (from 8: 1 concentrate) ( genitalia ) 500 mg n / a Lactobacillus lambosus 10 billion CFU n / a

n / a = Daily number is not determined.

Other Ingredients: Hypromellose (capsules), rice flour, magnesium stearate.

Fat Production Test Using the Composition of Table 3:

Differentiation of human bone marrow-derived MSC into adipocytes.

Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA). After thawing mononuclear cells were incubated with α-minimal essential medium (α-MEM, Invitrogen, Carlsbad, CA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Invitrogen, Carlsbad, Calif.) And 1% antibiotics and antifungal , Carlsbad CA). The cells were plated at 1-5 x10 ⁶ cell density per 100 cm ² dish. The culture was maintained in a 5% CO ₂ incubator at 37 ° C and the medium was changed after 48 hours and every 3-4 days thereafter. When the MSCs were dense, cells were harvested by addition of 0.25% trypsin / EDTA (Invitrogen, Carlsbad, Calif.). MSCs (2-3 passages) were plated in 75-cm ² flasks or 24-well plates and cultured in a-MEM with 20% FBS to a density of up to 2.0 x 10 ⁴ cells / cm ² . This medium was replaced with fat-producing medium, and cells were cultured for an additional 14 days. This fat-producing medium contained the COX-1 inhibitor (2-valeryloxybenzoic acid, Cayman, Ann Arbor MI) and the COX-2 inhibitor (3- (4- methylsulfonylphenyl) -4-phenyl- 20-HETE agonist (20-5,14-HEDE) or 20-OH-PGE ₂ and in conjunction with 20-HETE, 20-OH-PGE ₂ and in the presence or absence of DMEM-high glucose , Complete culture medium supplemented with 10% (v / v) FBS, 10 μg / ml insulin, 0.5 mM dexamethasone (Sigma-Aldrich, St. Louis, Mo.), and 1% antibiotic and antifungal agent (Invitrogen, Carlsbad, CA) . 20-HETE, 20-HETE agonist or 20-OH-PGE ₂ were added three times per week at 0.1 and 1 μM concentrations. The inhibitors of COX-1 and COX-2 were added three times per week in a volume of 100 [mu] M and 5 [mu] M, respectively.

Oil Red O dyeing.

On the 14th day of fat production, 0.21% Oil Red O (Sigma-Aldrich, St. Louis, MO) in 100% isopropanol was used. In summary, adipocytes were fixed in 10% formaldehyde, washed in Oil-red O for 10 min, rinsed in 60% isopropanol (Sigma-Aldrich, St. Louis, Mo.) and oil red O was added with 100% isopropanol , Eluted for 10 minutes and read at 490 nm for 0.5 seconds to measure the OD. MSC-derived adipocytes were measured with Oil red O staining (OD = 490 nm) after 14 days. Each Oil red O staining value was normalized to cell number (OD = 490 nm).

In the test, it was observed that the formulation of Table 3 in the Oil Red O staining test resulted in a statistically significant reduction in fat production as compared to untreated human MSC. In addition, the test showed that, in the absence of Lactobacillus lambusosus, the addition of other ingredients in the formulation of Table 3 did not result in any statistically significant reduction in fat production. The addition of Lactobacillus rhamnosus is thought to be a cause of synergy in combination with other ingredients in reducing fat production.

Anti-oxidative stress.

Percentages of live and dead cells were determined simultaneously by measuring intracellular esterase activity and plasma membrane integrity using the LIVE / DEAD® Viability / Cytotoxicity Assay Kit (Life Technologies). In summary, human MSCs were plated on 24-well plates. The next day, cells were treated with the components and 100 μM H ₂ O ₂ for 12 hours. Supernatant medium from each well was collected in microtubes, the cells were washed with 1X PBS, and the washings were collected in microtubes, respectively, to collect any floating or dead cells. Cells attached at the bottom of plate were incubated in 1X PBS in a 37 ° C incubator. The microtube was centrifuged at 2000 rpm for 3 minutes to obtain cell pellet. After removal of the supernatant, the cell pellet was resuspended in 1X PBS containing 2 [mu] M chalcene AM and 4 [mu] M ethidium / homodimer. The re-suspended cells were again poured into each well and the plates were incubated at 37 [deg.] C for 15 minutes. The cells were then photographed using a fluorescence microscope (Olympus IX71) at 10X magnification.

The anti-oxidative stress test showed that the other ingredients of the formulation of Table 3, when tested alone, did not show a significant improvement in cell viability after 12 hours exposure to H ₂ O ₂ . However, the formulations of Table 3 showed a noticeable improvement in cell viability after 12 hours exposure to H ₂ O ₂ . These test results further support the idea that the combination of the above components has a synergistic effect in protecting cells against oxidative stress and ultimate apoptosis.

It is also believed that the formulations of Table 3 will have beneficial effects on cartilage and osteogenic differentiation of human MSCs. Inflammation is detrimental to cartilage formation and cartilage cell formation from human MSCs. The formulation of Table 3 shows an anti-inflammatory effect as implied by a significant reduction in fat production in the experiments described above.

Illustrative embodiments are provided so that this application will fully convey the scope of the invention to those skilled in the art. In order to provide a thorough understanding of the embodiments of the present application, numerous specific details are provided, for example, examples of specific components, devices, and methods. It will be apparent to those skilled in the art that these specific details need not be used and that the illustrative embodiments may be embodied in many different forms and should not be construed as limiting the scope of the present application . In some illustrative embodiments, well-known processes, widely-known device structures, and widely-known techniques are not described in detail. Modifications, variations, and variations on some embodiments, materials, compositions and methods that have substantially similar results within the scope of the present invention can be made.

Claims (20)

Dietary supplements including:
Lactobacillus rhamnosus ;
ginger; And
Vitamin D. The dietary supplement according to claim 1, wherein the Lactobacillus laminocus comprises about 10 billion CFU of Lactobacillus lambosus . The dietary supplement according to claim 1, wherein the ginger comprises about 500 mg of ginger. The dietary supplement according to claim 1, wherein the ginger comprises about 8: 1 ginger root concentrate. The dietary supplement according to claim 1, wherein the vitamin D comprises about 1,000 IU of vitamin D. The dietary supplement according to claim 1, further comprising curcumin. 7. The dietary supplement according to claim 6, wherein the curcumin comprises about 350 mg of a turmeric root extract. The dietary supplement according to claim 1, further comprising a Boswellia extract. 9. The method of claim 8, wherein the boswellia extract comprises about 500 mg boswellia Dietary supplements characterized by including other Serra (Boswellia serrata) extract. The dietary supplement according to claim 1, further comprising curcumin and boswellia extract. The dietary supplement according to claim 1, further comprising a green tea extract. The method according to claim 11, green tea extract is about 98% polyphenols, 80% catechins, and standardized Camellia for catechin gallate in 50% epi-go And about 500 mg of green tea leaf extract of Camellia sinensis . The dietary supplement according to claim 1, further comprising a boswellia extract and a green tea extract. The dietary supplement according to claim 1, further comprising an Epimedium extract. The method according to claim 14, epi medium extracts the epitaxial medium for the standardization Ica Lin about 10% fraud tatum (Epimedium sagittatum ) extract of about 500 mg. The dietary supplement according to claim 1, further comprising curcumin and epimedium extract. A health problem aiding method associated with any one of joint support, aging, and sports medicine, including:
Administering a dietary supplement according to claim 1. Providing joint support and alleviating arthritic effects, including:
10. A method of administering a dietary supplement as claimed in claim 10. An aging effect mitigation method comprising:
13. The method of claim 13, wherein the dietary supplement is administered. A method of alleviating problems arising from either physical activity or athletic activity, including:
16. The method of claim 16, wherein the dietary supplement is administered.