KR20170061815A - Kit and automatic apparatus for isolating or purifying nucleic or various biological materials - Google Patents

Kit and automatic apparatus for isolating or purifying nucleic or various biological materials Download PDF

Info

Publication number
KR20170061815A
KR20170061815A KR1020150166773A KR20150166773A KR20170061815A KR 20170061815 A KR20170061815 A KR 20170061815A KR 1020150166773 A KR1020150166773 A KR 1020150166773A KR 20150166773 A KR20150166773 A KR 20150166773A KR 20170061815 A KR20170061815 A KR 20170061815A
Authority
KR
South Korea
Prior art keywords
magnet
mounting rod
width direction
cover
magnetic
Prior art date
Application number
KR1020150166773A
Other languages
Korean (ko)
Other versions
KR101755563B1 (en
Inventor
박승교
Original Assignee
주식회사 파나진
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 주식회사 파나진 filed Critical 주식회사 파나진
Priority to KR1020150166773A priority Critical patent/KR101755563B1/en
Publication of KR20170061815A publication Critical patent/KR20170061815A/en
Application granted granted Critical
Publication of KR101755563B1 publication Critical patent/KR101755563B1/en

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/026—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having blocks or racks of reaction cells or cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0418—Plate elements with several rows of samples

Abstract

The present invention relates to a kit and an automation apparatus for extracting or purifying nucleic acids or various biological materials capable of enhancing the collection rate of magnetic substances and firmly attaching magnets while slimming the structure of the magnets, , A mounting rod cover (20) for inserting the magnet mounting rod (10) into the inner space, a sample holder (20) for holding the mounting rod cover (20) The magnet mounting rod 10 is formed to have magnetic poles of the magnet 11 on both sides in the width direction at the lower end.

Description

[0001] KIT AND AUTOMATIC APPARATUS FOR ISOLATING OR PURIFYING NUCLEIC OR VARIOUS BIOLOGICAL MATERIALS FOR EXTRACTION OR REFINING NUCLEIC ACID OR VARIOUS BIOLOGICAL MATERIALS [0002]

The present invention relates to a kit and an automation apparatus for extracting or purifying a nucleic acid or various biological materials capable of increasing the collection rate of a magnetic material and firmly attaching magnets while reducing the structure in which a magnet is attached.

Apparatuses for extracting or purifying nucleic acid or biological material from various biological samples such as human tissues and blood have been widely used in a variety of fields such as medicine and biotechnology. Recently, mutation of a gene has been detected by amplification through a polymerase chain reaction BACKGROUND OF THE INVENTION As molecular diagnostic methods become popular, devices for efficiently separating or purifying nucleic acids or various biological materials are being developed in succession.

Such a device can be applied not only to humans but also to samples collected from animals, plants, insects, seaweeds, microorganisms, bacteria, fungi, viruses and the like.

In order to extract or purify nucleic acids or various biological materials, it is necessary to perform various steps such as lysis, binding, washing, and elutting. , There is a fear of infection at the time of use, and reliability is deteriorated due to the inconsistency in the quantity and quality of the separated or purified nucleic acid or various biological materials, and thus it has also been developed as an automated apparatus. As an advantageous method for application to an automation device among these devices, a magnetic material having a property of adsorbing nucleic acids or various biological materials, that is, a method using magnetic beads or magnetic particles is widely used in the field of molecular diagnostics.

Apparatuses using magnetic material include magnet mounting rods provided with magnets at the bottom, mounting rods covering the magnet mounting rods except for the top, wells for charging the magnetic material after the biological sample is dispensed, wells for dispensing the cleaning liquid, A well for dispensing an eluent, and a plurality of wells necessary for extraction or purification. The automated apparatus further includes a magnet mounting rod, a lifting means for the mounting rod cover, and a moving means for moving the multiwell. . In order to simultaneously extract or purify nucleic acids or various biological materials from a plurality of biological samples, a plurality of wells having a plurality of wells required for extraction or purification are provided in a plurality of rows and a mounting rod cover and a magnet mounting rod arranged in the number of rows are used .

However, according to the conventional apparatus, the cylindrical magnet having the magnetic pole on the upper surface and the lower surface is vertically mounted on the lower bottom surface of the magnet mounting rod. That is, the upper surface having the magnetic pole in the anode was fixed to the bottom surface of the magnet mounting rod. With such a magnet mounting structure, the magnetic flux density is high only around the bottom surface of the magnet, and the magnetic substance material is locally attracted only around the bottom surface. Therefore, the adsorption rate should be low and the adsorption time must be long. Even if the adsorption time is long, a considerable amount of residual magnetic material may remain, which may cause a problem affecting the performance of the polymerase chain reaction.

In order to solve the problem of lowering the adsorption rate, prolonging the adsorption time, and retaining the magnetic material, it is necessary to use a magnet having a large magnetic force. However, since the size of the magnet is large as the magnetic force is large, Which is undesirable.

Furthermore, when the magnet is fixed to the bottom surface of the magnet mounting rod by an adhesive method, there is a problem that the magnet is broken due to an impact or repeated use. Thus, although a rigid fixing structure can be employed, the manufacturing process becomes complicated and the slimming down will be restricted.

KR 10-0483684 B1 Apr. 07, 2005. KR 10-0720044 B1 2007.05.14.

Accordingly, a problem to be solved by the present invention is to provide a magnet mounting apparatus which can collect magnetic substance by using both of the poles of the magnet when the magnet is fixed to the lower end of the magnet mounting rod to improve the collection rate and slimness, And to provide a kit and an automation apparatus for extracting or purifying nucleic acids or various biological materials having durability.

In order to accomplish the above object, the present invention is characterized in that a magnet mounting bar 10 provided with a magnet 11 at a lower end is inserted into an inner space of a mounting rod cover 20 through an upper opening of a mounting rod cover 20 whose lower end is closed And the lower part of the mounting rod cover 20 is inserted into the well 31 into which the magnetic substance material is charged so that the nucleic acid of the sample contained in the well 31 or a magnetic substance to which various biological substances are adsorbed on the surface A kit for extracting or purifying nucleic acids or various biological substances by being collected at the lower end of a mounting rod cover (20) by magnetic force of a magnet (11), characterized in that the magnet mounting rod (10) (11). ≪ / RTI >

The magnet mounting bar 10 has a width direction through hole 12 formed at the lower end thereof and then a magnet 11 having a magnetic pole on both sides in the width direction is inserted and fixed in the width direction through hole 12, 11). ≪ / RTI >

The magnet mounting bar 10 is characterized in that a magnetic flux passage hole 13 in the width direction is formed on the width direction through hole 12 in parallel with the width direction through hole 12.

The lower portion of the width direction through hole 12 is open so that the lower portion of the magnet 11 faces the inner bottom surface of the mounting rod cover 20.

The lower bottom surface of the magnet mounting bar 10 and the bottom inner space bottom surface of the mounting rod cover 20 are in a shape that can be brought into close contact with each other and the bottom outer bottom surface of the mounting rod cover 20 and the bottom surface of the well 31 The inner bottom surfaces of the first and second plates have a shape that can be brought into close contact with each other.

The magnet mounting bar 10 is composed of a rod having a rectangular flat cross-sectional shape in which the thickness in the width direction is relatively small in comparison with the thickness in the longitudinal direction, and the magnetic pole area is relatively increased in comparison with the case where the magnetic pole is provided on both sides in the longitudinal direction, The mounting bar cover 20 has a rectangular flat cross-sectional shape so as to cover at least a portion where the magnet 11 is mounted at a uniform thickness in the magnet mounting bar 20, And the inner surface of the outer surface of the magnetic pole has an inner surface in which an interval between the inner surface and the surface having the magnetic pole is relatively larger than a distance between the inner surface and the surface having no magnet pole.

According to the present invention configured as described above, the nucleic acid or various biological substances are captured by the magnetic force of the magnetic poles provided on both sides in the width direction in the lower end of the magnet mounting rod, so that the collection rate is improved as compared with the prior art that is adsorbed by one magnetic pole.

In addition, since the structure of the present invention is capable of maintaining the area of the collecting surface formed on the mounting rod cover by the magnetic pole even if the width is reduced, compared with the prior art in which the area of the collecting surface is reduced when the width is reduced, Thus, it is easy to apply to narrow multiwells between wells such as a microplate for PCR (polymerase chain reaction), and has a favorable structure for multi-pipetting.

Further, since the magnet is inserted into the width direction through hole provided at the lower end of the magnet mounting rod, the magnet can be more firmly fixed than in the prior art in which the magnet is fixed to the lower end, The durability is ensured when the magnet mounting rod is inserted into the mounting rod cover.

Further, according to the present invention, magnetic flux lines are further concentrated on the collecting surface of a portion of the outer surface of the mounting rod cover that covers the magnetic pole of the magnet, thereby further improving the collection rate.

1 is a perspective view of a kit 1 according to an embodiment of the present invention;
Fig. 2 is a front view (b) showing a magnet mounting bar 10 after vertical incision in a side view (a) and a width direction (W) of the magnet mounting bar 10 shown in Fig. 1,
Fig. 3 is a top view (a) of the mounting bar cover 20 shown in Fig. 1, a front view (b) showing the mounting rod cover 20 after vertical incision in the width direction (W) (C) showing the mounting rod cover 20 after the vertical incision is made.
Fig. 4 is a top view (a) of the multi-well 30 shown in Fig. 1, a side view (b) showing the multiwell 30 after vertical incision in the longitudinal direction (W) (C) is a front view showing the multiwell 30 after the incision.
FIG. 5 is a view for explaining a state in which a magnetic material is adsorbed to the present invention and a conventional technique. FIG.
6 is a perspective view showing another embodiment of the magnet mounting bar 10. Fig.
FIG. 7 is a perspective view showing another embodiment of the multi-well 30. FIG.
FIG. 8 is an internal side view of an automation apparatus according to an embodiment of the present invention. FIG. 8 is a cross-sectional view of the multiwell, showing clearly the space to be dispensed such as a sample, a washing liquid, an eluting liquid, and the like.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. The detailed description of known functions and configurations incorporated herein will be omitted when it may obscure the subject matter of the present invention.

1 is a perspective view of a kit 1 according to an embodiment of the present invention.

2 is a side view (a) of the magnet mounting bar 10 shown in Fig. 1 and a front view (b) of the magnet mounting bar 10 cut perpendicularly in the width direction W. Fig.

3 is a top view (a) of the mounting bar cover 20 shown in Fig. 1, a front view (b) and a front view (b) of the mounting bar cover 20 cut vertically in the width direction (C) is a side view of the incision mounting bar cover 20.

Fig. 4 is a top view (a) of the multiwell 30 shown in Fig. 1, a side view (b) and a width direction (W) of the vertically incised multiwell 30 in the longitudinal direction (L) 30 is a front view (c) of FIG.

1 to 4, a kit 1 according to an embodiment of the present invention is a kit used for extracting or purifying nucleic acid or various biological materials from a biological sample, and includes a magnet 11 A mounting rod cover 20 formed so that the magnet mounting rod 10 and the magnet mounting rod 10 can be vertically erected and inserted into the inner space 21 and the mounting rod cover 20 are vertically erected and inserted A plurality of wells 30 are formed in a plurality of rows in the width direction W and a plurality of wells 31 arranged in the longitudinal direction L are provided in the longitudinal direction L, .

Here, the magnet mounting rod 10, the mounting rod cover 20 and the well 31 are generally formed of a non-magnetic material so as not to be magnetized by the magnet 11.

In order to facilitate understanding of the kit 1 configured as described above, an example of the use method will be described.

First, a biological sample is dispensed in accordance with the order of the plurality of wells 31 arranged in the longitudinal direction L, and a reaction well in which a magnetic substance (magnetic bead, magnetic particle) is put into a biological sample, a washing well in which a washing liquid is dispensed, And the eluent wells are sequentially prepared.

Next, the lower portion of the mounting rod cover 20 is immersed in the biological sample which has been dispensed into the reaction well, and then the mounting rod cover 20 is vibrated up and down to stir the magnetic substance into the biological sample. Thus, the nucleic acid or various biological substances of the biological sample are adsorbed on the surface of the magnetic substance substance.

The magnetic substance is attracted by the magnetic force of the magnet 11 attached to the lower end of the magnet mounting bar 10 and the surface of the mounting rod cover 20 .

Next, the mounting rod cover 20 in a state in which the magnet mounting rod 10 is inserted is lifted and immersed in a cleaning liquid in the cleaning well, whereby a magnetic substance, on which nucleic acids or various biological substances are adsorbed on the surface, The cleaning wafers can be moved to the cleaning wafers of the next order.

In the cleaning well, the magnet mounting rod 10 is taken out of the mounting rod cover 20 and then stirred with the mounting rod cover 20. Thereafter, the magnet mounting rod 10 is inserted again into the mounting rod cover 20, Is collected again. Then, with the magnet mounting bar 10 inserted, the mounting rod cover 20 is immersed in an eluting solution dispensed into the next eluting well.

Although the method of using the present invention has been described with reference to the process of collecting and moving the magnetic material for understanding the present invention, the present invention is not limited thereto.

Hereinafter, the mounting structure of the magnet 11, which is a feature of the present invention, and the shapes of the magnet mounting bar 10, the mounting rod cover 20 and the multiwell 30 will be described in detail.

The magnet mounting bar 10 has a width direction through hole 12 formed at the lower end thereof and then a magnet 11 fitted in the width direction through hole 12 is inserted and fixed, Both sides in the width direction W of the housing 11 are exposed.

According to a specific embodiment of the present invention, the magnet mounting bar 10 is composed of a rod formed by vertically extending vertically, and is formed into a rectangular shape having a thickness in the width direction (W) Sectional shape, so that the two-sided spacing of the width direction W is relatively small and the area is relatively wide, as compared with both sides in the longitudinal direction L. Accordingly, the magnets 11 can be formed and mounted so as to expose the magnets 11 having a large area on both sides in the width direction (W).

Here, the magnet 11 is a magnet having magnetic poles (N poles, S poles) on both sides in the width direction W. In the embodiment of the present invention, the magnet 11 has a magnetic pole on both flat surfaces in the width direction A width direction through hole 12 corresponding to the diameter of the magnet is formed at the lower end of the magnet mounting bar 20 and is inserted and fixed.

Thus, the magnet mounting bar 10 is supported on both sides of the width direction W by the characteristic of the magnet 11 that enters the S-pole of the other surface after the magnetic force lines come out from the N-pole of one surface and pass the outside of the magnet 11. [ The magnetic flux density of the magnetic poles of the exposed magnet 11 is relatively large compared to the other portions.

In addition, when the magnetic pole is provided on both sides of the width direction W at the lower end of the magnet mounting bar 10, the area of the magnetic pole can be made relatively larger than that having the magnetic pole on both sides in the longitudinal direction L. In other words, the lower end structure of the magnet mounting bar 10 can occupy a very large area occupied by the magnetic poles along the circumferential direction. This shows that the lower end of the magnet mounting bar 10 can be made slimmer if the area occupied by the magnetic pole is maximized.

On the other hand, the lower structure of the magnet mounting bar 10 is provided with a magnetic flux passage hole 13 formed in the lateral direction through the through hole 12 in the width direction in parallel with the widthwise through hole 12. The magnetic force lines passing through the magnets 11 out of the magnetic poles of the magnetic poles 11 entering the magnetic poles of the other surface through the magnetic poles of the other surface do not pass through the magnet mounting rods 10 but pass through the air so that the magnetic permeability of the magnet mounting rods 10 permeability.

1 and 2, the magnet mounting rods 10 are arranged to be spaced apart from each other in the width direction W, but the upper portions of the magnet mounting rods 10 may be connected to each other. The bundling of the plurality of magnet mounting rods 10 as described above is for simultaneously treating a plurality of samples by one operation.

The mounting bar cover 20 is provided with an inner space 21 having a lower end closed and an upper end opened so that the magnet mounting rod 10 can be vertically installed and inserted into the inner space 21 through the upper opening 22 It is sufficient that the magnet mounting bar 10 can be inserted into the inner space 21 except for the upper end thereof in order to take out the magnet mounting bar 10 from the inner space 21. [

The portion of the magnet mounting bar 10 on which the magnet 11 is mounted is brought into contact with the inner wall of the inner space 21 as much as possible so that the magnetic force lines of the magnet 11 Through the widthwise outer surface.

That is, the mounting rod cover 20 is configured such that the magnet mounting rod 10 can be inserted into and removed from the inner space 21, and at least when the magnet mounting rod 10 is inserted into the inner space 21, It is sufficient that the lower bottom surface of the magnet mounting bar 10 is in close contact with the bottom surface of the inner space 21. [

In the embodiment of the present invention, a uniform thickness is provided so as to surround a portion where the magnet 11 is present in a uniform thickness, and a rectangular shape is obtained in a flat section as in the case of the magnet mounting bar 10. Therefore, the magnetic flux density at the portion where the magnet 11 is present is substantially uniform.

The mounting bar covers 20 are arranged in the width direction by the number of the magnet mounting rods 10 so that the plurality of magnet mounting rods 10 can be inserted and removed one by one, 23).

The multi-well 30 has a shape in which a plurality of wells 31 (wells) are spread in the lateral and longitudinal directions. Here, each of the wells 31 is provided with an inner space having a lower end closed and an upper end opened, but the mounting rod cover 20 can be vertically erected and inserted into the interior.

The plurality of wells 31 may include a number or a number of preliminary numbers for performing each step of the method for extracting or purifying nucleic acids or various biological substances as described above, for example, .

The plurality of wells 11 arranged in the longitudinal direction for improving the efficiency or for multipipetting are provided in a plurality of rows in the width direction, and the number of rows in the width direction is the number of the mounting rod covers 20 (or the number of the magnet mounting rods ). Thus, a plurality of mounting rod covers 20 arranged in the width direction can be inserted or withdrawn one by one in the plurality of wells 11 in the width direction.

According to the embodiment of the present invention, the outer surface of the mounting bar cover 20 has a rectangular flat cross section in which the interval between the outer surface of the mounting bar cover 20 and the surface on which the magnetic pole 11 is provided is larger than the interval between the surface And each well 31 was formed so as to have an inner surface of a shape.

Accordingly, when the biological sample or the cleaning liquid dispensed in the well 31 is brought into contact with the cover rod cover 20 to collect the magnetic material, a relatively large amount of sample or cleaning liquid .

5, the structure of the magnet mounting bar 10 in which the magnet 11 is mounted and the collecting efficiency of the magnetic substance material by the structure of the mounting rod cover 20 and the multi-well 30 will be described.

FIG. 5 is a sectional view showing a state in which the biological sample is dispensed and the mounting rod covers 20 and 20 'are inserted into the wells 31 and 31' into which the magnetic substance is charged, Sectional view of a state in which the magnetic material is adsorbed in a state where the magnet mounting rods 10 and 10 'are inserted into the inner spaces 21 and 21'

5 (a), the magnetic substance P 'is collected on the lower bottom surface of the mounting bar cover 20 substantially because the magnetic poles of the magnet 11 are present on the upper and lower sides. In this case, in order to increase the collection rate, the lower bottom surface where the magnetic material P 'is collected should be widened or the magnet 11' having a large magnetic force must be provided.

On the other hand, according to the present invention shown in Fig. 5 (b). Magnetic substance P is collected on both sides in the width direction in the lower end of the mounting bar cover 20 because the magnetic pole exists on both sides in the width direction. Accordingly, the collection area is relatively large as compared with the conventional technique while using one magnet 11, and the collection rate is relatively large. As a result, the present invention can be configured to have a collection rate equal to or higher than that of the prior art, even if the configuration is made slim.

In addition, when the present invention is applied to a PCR Microplate in which the interval between the adjacent wells 31 in the width direction is narrowed and the width of the well 31 is made small, the well 31 is formed as shown in FIG. 1 or FIG. The area occupied by the magnetic poles of the magnet 11 may not be reduced even if it is small in the width direction, so that the collection rate of the magnetic substance material can be maintained as it is.

On the other hand, when the width of the well 31 is reduced, the area of the irregularity is reduced and the collection rate is also reduced. Therefore, in order to satisfy the collection rate, a magnet having a large magnetic force must be used or extended in the longitudinal direction.

Accordingly, the present invention is advantageous in that it can be downsized and slimmed as compared with the prior art, and it is possible to maintain the collection rate with respect to the magnetic material even if it is downsized and slim.

1 to 4, the inner bottom surface of the well 31, the inner bottom surface and outer bottom surface of the mounting rod cover 20, and the bottom surface of the magnet mounting rod 10 are viewed in the width direction It is semicircular in shape when viewed, but may be triangular.

The lower bottom surface of the magnet mounting bar 10 can be brought into close contact with the bottom surface of the inner space 21 of the mounting rod cover 20 and the inner bottom surface of the well 31 and the outer bottom surface of the mounting rod cover 20 It is preferable to be able to uniformize the distance.

When the kit 1 of the present invention is used to bring the outer bottom surface of the mounting rod cover 20 into close contact with the inner bottom surface of the well 31, the sample (or washing liquid) So that the nucleic acid or various biological samples of the sample can be collected and collected in an amount minimized in the sample.

Fig. 6 is a perspective view showing another embodiment of the magnet mounting bar 10. Fig.

6, the lower portion of the width direction through hole 12 is open so that the lower portion of the magnet 11 can face the bottom surface of the inner space 21 of the mounting rod cover 20 have.

As a result, the lower end outer surface of the mounting bar cover 20 has the collecting surface formed on both sides in the width direction (i.e., the outer surface where magnetic flux density capable of collecting the magnetic substance material is generated) at a lower position. This makes it possible to increase the collection rate with respect to the magnetic material by lowering the leakage of the magnetic force lines passing through the magnet mounting rods 10 so as to reduce the width of the mounting rods 20 in the width direction The magnetic flux density of the collecting surface formed on both surfaces is further increased.

On the other hand, as shown in Fig. 6 (a), a structure for exposing the magnet 11 to an acute angle range, a structure for exposing the magnet 11 by the lower half as shown in Fig. 6 (b) However, it is possible to expose the magnet 11 in a range exceeding half. In the case of employing a structure in which the magnet 11 is exposed in a half or more than half of the magnet 11, the magnet 11 should be fixed to the lower end of the magnet mounting bar 10, and for example, an adhesive may be used. When the magnet 11 is exposed within an acute angle range, it is preferable to securely fix the magnet 11 using an adhesive.

On the other hand, as shown in FIG. 6, when the lower portion of the magnet 11 is exposed, the exposed portion has a shape to be brought into close contact with the bottom surface of the inner space 21 of the mounting rod cover 20.

7 is a perspective view showing another embodiment of the multi-well 30. FIG.

7, a plurality of unit well sets 30a of ppt type having only one row of the wells 31 in the longitudinal direction L are arranged in a required number in the width direction W to constitute the multiwell 30 . Here, the unit well set 30a may be a number or a preliminary number for performing each step of a method of extracting or purifying nucleic acid or various biological materials from a biological sample, And they can be used as multi wells shown in Fig. 3 by arranging a plurality of them in the width direction (W).

On the other hand, when extracting or purifying a sample of biological sample, only one unit well set 30a may be used.

8 is an internal side view of the automatic apparatus 2 in which the kit 1 is housed.

8, the automatic apparatus 2 includes a plurality of magnet mounting rods 10 arranged in the width direction at the same time, and simultaneously mounting and dismounting the plurality of magnet mounting rods 10 in a one-to-one manner to a plurality of mounting rod covers 20 arranged in the width direction, A cover lifting means 52 for simultaneous lifting and lowering of the plurality of mounting rod covers 20 and simultaneously arranging the plurality of mounting rod covers 20 in a single direction on a plurality of wells 11 arranged in the width direction, The sliding means 53 is mounted inside the case 40. The sliding means 53 is mounted on the inside of the case 40.

Here, by moving the multi-well 30 with the sliding means 53, the well into which the mounting rod cover 20 is inserted into the aligned wells in the longitudinal direction in the multi-well 30 can be changed.

Although not shown in the drawings, the mounting bracket bracket for fixing the plurality of magnet mounting rods 10 in the width direction, the mounting rod lifting means 51 for moving the mounting bracket bracket, the plurality of mounting rod covers 20, And a block on which the mounting bracket lifting and lowering means 52 for lifting and moving the cover bracket are mounted are moved along the longitudinal direction by the sliding means 53 and the multiwell 30 ) Can also be used. That is, the sliding means 30 is satisfactory if the multi-well 30 is relatively moved in the longitudinal direction relative to the block.

Here, the mounting rod lifting and lowering means 51 and the cover lifting means 52 can be constructed by employing a general device for raising and lowering the bracket along vertical rails. The sliding means 53 moves the object to be moved back and forth A general apparatus can be employed, so that a detailed description thereof will be omitted.

The operation of the automatic apparatus 2 for extracting or purifying nucleic acids or various biological substances is carried out by the operation of each of the operation steps for carrying out the extraction or purification by the mounting rod lifting and lowering means 51 to the controller 60 provided in the case 60, , The cover lifting means (52) and the sliding means (53), and these operations are known in the prior art and will not be described in detail.

The case 40 is provided with a door 41 on one side in the longitudinal direction so that the multiwell 30 can be inserted into the case 40 through the door 41 and the magnet mounting rod 10 and the mounting rod cover 20 Is also possible through the door (41).

Since the automatic apparatus 2 is structured by mounting the kit 1 described above, it is possible to increase the collection rate of the magnetic substance material while increasing the magnet mounting rod 10, the mounting rod cover 20, (30) can be slim and miniaturized, and the case (40) can be made compact and can be configured as an automation device which is easy to carry or move.

In addition, even when a case of the same size as the prior art is used, the multi-well in which the number of the wells 31 in the width direction (or the number of the magnet mounting rods and the mounting rod covers) is relatively larger is accommodated, It is also possible to adopt a structure in which a plurality of projections are accommodated in the width direction.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, . ≪ / RTI > Accordingly, such modifications are deemed to be within the scope of the present invention, and the scope of the present invention should be determined by the following claims.

1: Kit 2: Automation device
10: Magnet mounting rod
11: magnet 12: width direction through hole 13: magnetic flux passage hole
20: Mounting rod cover
21: inner space 22: upper opening 23: plate
30: Multi well 31: Well
40: Case 41: Door
50: Driving device
51: Mounting rod lifting means
52: Cover lifting means
53: Sliding means
60: controller

Claims (7)

It is possible to insert the magnet mounting rod 10 provided with the magnet 11 at the lower end into the space inside the mounting rod cover 20 through the upper opening of the mounting rod cover 20 with the lower end closed, The lower portion of the mounting rod cover 20 is inserted into the well 31 into which the substance is introduced so that the nucleic acid of the sample contained in the well 31 or the magnetic substance in which various biological substances are adsorbed on the surface is immobilized by the magnetic force of the magnet 11 In a kit for extracting or purifying nucleic acid or various biological materials by being collected at the lower end of the cover 20,
Wherein the magnet mounting bar (10) has magnetic poles of the magnet (11) on both sides in the width direction of the lower end. The method according to claim 1,
The magnet mounting bar 10 has a width direction through hole 12 formed at the lower end thereof and then a magnet 11 having a magnetic pole on both sides in the width direction is inserted and fixed in the width direction through hole 12, Lt; RTI ID = 0.0 > 11). ≪ / RTI > 3. The method of claim 2,
Characterized in that the magnet mounting bar (10) has a magnetic flux passage (13) in the width direction formed on the width direction through hole (12) in parallel with the widthwise through hole (12). 3. The method of claim 2,
The lower portion of the width direction through hole (12) is open so that the lower portion of the magnet (11) faces the inner bottom surface of the mounting rod cover (20). The method according to claim 1,
The lower bottom surface of the magnet mounting bar (10) and the bottom space inner bottom surface of the mounting rod cover (20) can be in close contact with each other,
Wherein the lower end outer bottom surface of the mounting rod cover (20) and the inner bottom surface of the well (31) have a shape that can be brought into close contact with each other. The method according to claim 1,
The magnet mounting bar 10 is composed of a rod having a rectangular flat cross-sectional shape in which the thickness in the width direction is relatively small in comparison with the thickness in the longitudinal direction, and the magnetic pole area is relatively increased in comparison with the case where the magnetic pole is provided on both sides in the longitudinal direction,
The mounting bar cover 20 has a rectangular flat cross-sectional shape so as to cover at least a portion where the magnet 11 is mounted at a uniform thickness in the magnet mounting bar 20,
Wherein the well 31 has an inner surface in which an outer surface of the mounting rod cover 20 has a space between the inner surface and the surface on which the magnetic pole is provided, Kits. A plurality of wells 11 arranged in the longitudinal direction in a plurality of rows along the width direction and a plurality of wells 11 arranged in the width direction and arranged in the width direction can be inserted or withdrawn one to one And a plurality of magnet mounting rods (10) arranged in a plurality in the width direction and capable of being inserted or withdrawn one by one to a plurality of mounting rod covers (20) Lt; / RTI >
The multiwell 30 is inserted through the door 41 provided at one side in the longitudinal direction,
Mounting rod lifting means (51) for simultaneously lifting and lowering a plurality of magnet mounting rods (10) simultaneously to and from a plurality of mounting rod covers (20), a plurality of mounting rod covers A cover lifting means 52 for lifting and lowering the mounting rod cover 20 in the longitudinal direction by relative movement between the mounting rod cover 20 and the multiwell 30 in the longitudinal direction, (53) for changing the well to be formed,
Wherein said kit is as set forth in any one of claims 1 to 6.
KR1020150166773A 2015-11-26 2015-11-26 Kit and automatic apparatus for isolating or purifying nucleic or various biological materials KR101755563B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020150166773A KR101755563B1 (en) 2015-11-26 2015-11-26 Kit and automatic apparatus for isolating or purifying nucleic or various biological materials

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020150166773A KR101755563B1 (en) 2015-11-26 2015-11-26 Kit and automatic apparatus for isolating or purifying nucleic or various biological materials

Publications (2)

Publication Number Publication Date
KR20170061815A true KR20170061815A (en) 2017-06-07
KR101755563B1 KR101755563B1 (en) 2017-07-10

Family

ID=59223538

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020150166773A KR101755563B1 (en) 2015-11-26 2015-11-26 Kit and automatic apparatus for isolating or purifying nucleic or various biological materials

Country Status (1)

Country Link
KR (1) KR101755563B1 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190031999A (en) * 2017-09-19 2019-03-27 (주)로봇앤드디자인 Dna processing apparatus
KR20200074684A (en) * 2018-12-17 2020-06-25 주식회사 제놀루션 Single Cartridge and Extraction Instrument for Nucleic Acid
KR20200110829A (en) * 2019-03-18 2020-09-28 주식회사 파나진 Nucleic acid concentration and extraction device
EP3828548A4 (en) * 2018-07-26 2022-03-30 Bioneer Corporation Target material extraction apparatus with replacement of magnetic bar block allowed
US11634703B2 (en) * 2018-03-02 2023-04-25 Psomagen, Inc. Method and system for high-throughput particle handling by use of magnetic fields and device

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102110647B1 (en) * 2018-05-03 2020-05-14 주식회사 파나진 Nucleic acid Extraction System and Nucleic acid Extraction Method from Large Volume Sample

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI120863B (en) * 2002-10-18 2010-04-15 Biocontrol Systems Inc Magnetic transfer method and microparticle transfer device
JP2010506172A (en) * 2006-10-06 2010-02-25 プロメガ・コーポレーション Apparatus and method for separating magnetic particles from solution

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190031999A (en) * 2017-09-19 2019-03-27 (주)로봇앤드디자인 Dna processing apparatus
US11634703B2 (en) * 2018-03-02 2023-04-25 Psomagen, Inc. Method and system for high-throughput particle handling by use of magnetic fields and device
EP3828548A4 (en) * 2018-07-26 2022-03-30 Bioneer Corporation Target material extraction apparatus with replacement of magnetic bar block allowed
KR20200074684A (en) * 2018-12-17 2020-06-25 주식회사 제놀루션 Single Cartridge and Extraction Instrument for Nucleic Acid
KR20200110829A (en) * 2019-03-18 2020-09-28 주식회사 파나진 Nucleic acid concentration and extraction device

Also Published As

Publication number Publication date
KR101755563B1 (en) 2017-07-10

Similar Documents

Publication Publication Date Title
KR101755563B1 (en) Kit and automatic apparatus for isolating or purifying nucleic or various biological materials
US5976369A (en) Magnetic separation apparatus
US7329488B2 (en) Kit for separating and purifying nucleic acids or various biological materials, and system for automatically performing separation or purification of biological materials using the same
ES2674264T3 (en) Method and device for microparticle treatment
EP2565260B1 (en) Automatic biological sample purification device having a magnetic-field-applying unit, a method for extracting a target substance from a biological sample, and a protein expression and purification method
EP2871460B1 (en) Kit for nucleic acid extraction, and nucleic acid extractor
US8685322B2 (en) Apparatus and method for the purification of biomolecules
US20130236903A1 (en) Automatic necleic acid purification apparatus and method for aerosol-protecting
US11242519B2 (en) Discontinuous wall hollow core magnet
US20130037471A1 (en) Methods and Device Employing Centrifugal Force
EP0589636B1 (en) Magnetic separation method and apparatus therefor
US10441957B2 (en) Magnetic particle separating device, and method of separating and purifying nucleic acid or protein using same
CN109562374B (en) Sample preparation rack and method of use thereof
KR101282841B1 (en) Apparatus and method for isolating nucleicacids or biological materials
US20020070173A1 (en) Apparatus and method for use in magnetic separation of magnetically attractable particles in a liquid
KR102225639B1 (en) Nucleic acid concentration and extraction device
JP2011234671A (en) Nucleic acid extraction device
US8129174B2 (en) Separating method and an apparatus performing such a method
WO2016134870A1 (en) Apparatus and method for the analysis; isolation and/or enrichment of target structures in a fluid sample
CN110945363A (en) Module for transferring magnetic beads, automated system comprising the module, and method for extracting nucleic acid using the module
KR102110647B1 (en) Nucleic acid Extraction System and Nucleic acid Extraction Method from Large Volume Sample
CN203355863U (en) Magnetic separation device for separating biological material
EP3759244B1 (en) Method and system for high-throughput particle handling by use of magnetic fields
KR20210108813A (en) Mixing rod array and multi well cartridge for apparatur of extracting nucleic acid
US20140024136A1 (en) Magnetic particle separator

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
AMND Amendment
E601 Decision to refuse application
AMND Amendment
X701 Decision to grant (after re-examination)
GRNT Written decision to grant