KR20170054116A - A pharmaceutical composition comprising extract from germinated gemmule of bean for preventing or treating obesity - Google Patents
A pharmaceutical composition comprising extract from germinated gemmule of bean for preventing or treating obesity Download PDFInfo
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- KR20170054116A KR20170054116A KR1020150156867A KR20150156867A KR20170054116A KR 20170054116 A KR20170054116 A KR 20170054116A KR 1020150156867 A KR1020150156867 A KR 1020150156867A KR 20150156867 A KR20150156867 A KR 20150156867A KR 20170054116 A KR20170054116 A KR 20170054116A
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- Prior art keywords
- extract
- obesity
- composition
- soybean
- preventing
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Abstract
The present invention relates to a pharmaceutical composition for preventing or treating obesity, which comprises a soybean germinated embryo extract, and more specifically, to a pharmaceutical composition for preventing or treating obesity comprising a soybean germinated embryo extract or a fraction thereof, To a food composition for preventing or ameliorating obesity comprising a soybean germinated embryo extract or a fraction thereof, and a feed composition.
Since the soybean germinated embryo extract of the present invention inhibits the differentiation of adipocytes and reduces the size thereof and converts white adipocytes into brown adipocytes, it suppresses accumulation of unnecessary fat in vivo, and thus is useful for preventing or treating obesity .
Description
The present invention relates to a pharmaceutical composition for preventing or treating obesity, which comprises a soybean germinated embryo extract, and more specifically, to a pharmaceutical composition for preventing or treating obesity comprising a soybean germinated embryo extract or a fraction thereof, To a food composition for preventing or ameliorating obesity comprising a soybean germinated embryo extract or a fraction thereof, and a feed composition.
Obesity or obesity is a state in which excess fat is accumulated in the body and refers to a state in which the number of adipocytes in adipose tissue is increased or the size of adipocyte is increased and fat is increased. Although obesity itself may not be a problem, the resulting social impairment and secondary complications caused by excessive fat are serious. For example, obesity causes diseases such as hypertension, hyperlipidemia, heart disease, diabetes, atherosclerosis, fatty liver, joint disorders, arthritis, sexual dysfunction, and cancer. Particularly, since the fat cells once formed are reduced in size, they are not removed naturally and remain permanently in the body, so that interest in the prevention and treatment of obesity is increasing.
Balanced diet and regular exercise are necessary to prevent or treat obesity and maintain proper weight. Recently, there have been cases where obesity is treated by taking drugs that reduce fat absorption. Such diet drugs may cause side effects such as cardiovascular diseases such as hypertension and stroke, Occasionally. In the case of severe obesity, surgical procedures such as surgery to remove fat or gastroplasty or stomach band insertion to limit the amount of food that can digest the body are performed, but the side effects of the surgery or complications resulting therefrom are reported And even deaths have been reported.
Accordingly, it is urgently required to develop a therapeutic agent for obesity which has less toxicity and side effects. Recently, alternative therapies using various active ingredients derived from natural products such as herbal medicines and foods or methods for producing various extracts have been studied. Examples thereof include a composition for prevention and treatment of obesity including extracts of herbal medicines (Korean Patent Laid-Open Publication No. 2015-0105563) or a composition for improving, preventing or treating obesity including garlic and herbal extract (Korean Patent Laid- 0085543) have been developed. However, development of a natural pharmaceutical composition or a raw material thereof, which has superior therapeutic effects and less side effects than conventional synthetic pharmaceutical compositions for obesity, has not yet been developed.
Under these circumstances, the present inventors have made intensive research to develop a therapeutic agent for obesity using a natural-derived component, and as a result, it has been found that an extract of a soybean germinated embryo produced by germinating an abandoned soybean embryo in the process of producing a processed product using soybean, Inhibiting the differentiation, reducing the size of adipocytes, and converting white adipocytes into brown adipocytes, thereby inhibiting the accumulation of unnecessary fats in vivo. Thus, the present invention has been completed.
It is an object of the present invention to provide a pharmaceutical composition for preventing or treating obesity comprising a soybean germinated embryo extract or a fraction thereof.
It is another object of the present invention to provide a method of preventing or treating obesity comprising the step of administering the composition to a subject other than a human.
It is still another object of the present invention to provide a food composition for preventing or ameliorating obesity comprising a soybean germinated embryo extract or a fraction thereof.
It is another object of the present invention to provide a feed composition for preventing or ameliorating obesity comprising a soybean germinated embryo extract or a fraction thereof.
One aspect for achieving the above object is to provide a pharmaceutical composition for preventing or treating obesity comprising a soybean germinated embryo extract or a fraction thereof.
The term "soybean germinated embryo" of the present invention means a germinated embryo obtained by germinating an embryo isolated from soybean. A method for producing a soybean germinated embryo has been known from previous studies of the present inventors (Korean Laid-Open Patent Application No. 2013-0057173). Specifically, the soybean germinated embryo may be obtained by germinating an embryo isolated from soybean, But is not limited thereto. The effect of treating the soybean germinated embryo extracts with obesity has not been known at all and has been first identified by the present inventors.
The soybean germinated embryo extract of the present invention may be obtained by extracting the soybean germinated embryo with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.
The term "extract " of the present invention means an extract obtained by extracting a soybean germinated embryo, a diluted solution or concentrate of the extract, a dried product obtained by drying the extract, a preparation or a purified product of the extract, Extracts themselves and extracts of all formulations which can be formed using extracts.
In the soybean germinated embryo extract of the present invention, the method for extracting the soybean germinated embryo is not particularly limited and may be carried out according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.
In the present invention, the kind of the extraction solvent used for extracting the soybean germinated embryo is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water, alcohol, and a mixed solvent thereof. These solvents may be used alone or in combination. When an alcohol is used as a solvent, an alcohol having 1 to 4 carbon atoms can be specifically used.
The term "fraction " of the present invention means a product obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.
The fractionation method for obtaining the fraction in the present invention is not particularly limited and may be carried out according to a method commonly used in the art. As a non-limiting example of the above-mentioned fractionation method, there can be mentioned a method of treating a soybean germinated embryo extract of the present invention with a predetermined solvent to obtain a fraction from the extract.
The kind of the fraction solvent used for obtaining the fraction in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fraction solvent include polar solvents such as water and alcohol; And non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of one or more. When an alcohol is used in the fraction solvent, an alcohol having 1 to 4 carbon atoms can be specifically used.
The soybean germination germ extract of the present invention may inhibit the differentiation of adipocytes.
The term "differentiation of adipocytes" of the present invention means that the adipocyte precursor cells are converted into adipocytes, and for the purpose of the present invention, the adipocyte accumulates fat in the cells.
In one embodiment of the present invention, the soybean germination germ extract was found to reduce fat contained in adipocytes to a similar extent as the CLA (conjugated linoleic acid) treated group, which is also used as a weight control health functional food, It was confirmed that the effect was more excellent than the extract obtained by germinating itself (FIG. 2). This suggests that the extract can effectively be used for the treatment of obesity because it exhibits an effect of removing excess accumulated fat.
In addition, the soybean germ extract of the present invention may reduce the size of adipocytes.
The term "size of fat cells" of the present invention means the diameter of fat cells. Since the size of the cells is determined by the amount of accumulated fat in the cells, the decrease in the size of the fat cells may be a decrease in the amount of fat in the cells.
In one embodiment of the present invention, the extract was administered to an animal model of obesity induced by menopause. As a result, it was confirmed that the diameter including the short axis and the long axis length of the adipocyte decreased (FIG. 4). This suggests that the extract has an effect of reducing the amount of fat in adipocytes, and thus can be effectively used for the treatment of obesity.
In addition, the soybean germination extract of the present invention may be one which converts white adipocytes into brown adipocytes.
The term "white adipocyte " of the present invention means a fat cell that stores fat, and is a cell that causes adult diseases such as diabetes and obesity.
The term "brown adipocyte" of the present invention means a cell that burns fat as energy, and plays a role in thermoregulation and energy production.
When white adipocytes are converted to brown adipocytes, the amount of fat burned is higher than the amount of fat stored in the body, so it is easier to maintain weight even if the same amount of food is consumed.
In one embodiment of the present invention, administration of the extract to obesity-induced animal models of menopause resulted in an increase in the number of brown adipose cells and a decrease in the size of white adipocytes 5). This suggests that the extract can effectively be used for the treatment of obesity because it exhibits an effect of suppressing the accumulation of fat by saturating brown adipocytes with white adipocytes.
The term "obesity" of the present invention means excessive fat tissue in the body. A man's body fat is over 25% of body weight, a woman is over 30% of body weight is called obesity, and when the amount of calories consumed by the food is greater than the calories consumed by moving the body, obesity occurs. When obesity occurs, it appears to be fat in appearance, can easily breathe, and may be accompanied by joint pain, diabetes, and high blood pressure.
The term "prevention" of the present invention means all the actions of suppressing or delaying obesity by administration of a pharmaceutical composition containing the extract of the present invention.
The term "treatment" of the present invention means any suspicion of obesity and all symptoms that the symptoms of the onset individuals are alleviated or changed by administering the pharmaceutical composition.
The pharmaceutical composition of the present invention may contain the extract in an amount of 0.0001 to 50% by weight based on the weight of the total composition, and may include, but is not limited to, 0.01 to 10% by weight.
The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier, excipient or diluent conventionally used in the production of a pharmaceutical composition, and the carrier may include a non-naturally occuring carrier .
The term "pharmaceutically acceptable" of the present invention means that it exhibits properties that are not toxic to the cells or humans exposed to the composition.
Specifically, the pharmaceutical composition may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterilized injection solutions according to a conventional method . In the present invention, the carrier, excipient and diluent which may be contained in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, Calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose or lactose lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. As a base for suppositories, witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like can be used.
One aspect for achieving the above object is to provide a method for preventing or treating obesity comprising the step of administering the composition to a subject other than a human.
The term "administering" of the present invention means introducing a given substance into an individual in a suitable manner.
The term "individual" of the present invention means all animals such as rats, mice, livestock and the like, including humans who have developed or are capable of developing obesity. As a specific example, it may be a mammal including a human.
The method for preventing or treating obesity according to the present invention may specifically include the step of administering to a subject other than a human in a pharmaceutically effective amount a pharmaceutical composition for preventing or treating obesity comprising a soybean germinated embryo extract or a fraction thereof have.
The term "pharmaceutically effective amount" of the present invention means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment and not causing side effects. The effective dose level is determined by the sex, age And other medical fields, including drugs used in combination or concurrently, with respect to body weight, health status, type of disease, severity, activity of the drug, sensitivity to the drug, method of administration, administration time, route of administration, Can be readily determined by those skilled in the art according to well known factors.
Specifically, the composition of the present invention may be administered at a dose of 0.0001 to 100 mg / kg body weight per day, more specifically 0.001 to 100 mg / kg body weight, based on the solid content. The administration may be such that the recommended dose is administered once a day or divided into several doses.
In the method for preventing or treating obesity of the present invention, the administration route and method of administering the composition are not particularly limited, and any route of administration and administration may be used as long as the composition containing the composition can reach the desired site It is possible to follow the method. Specifically, the composition may be administered orally or parenterally through various routes. Non-limiting examples of routes of administration include oral, rectal, topical, intravenous, intraperitoneal, intramuscular, intraarterial, transdermal, Intramuscularly or through inhalation or the like.
One aspect of the present invention provides a food composition for preventing or ameliorating obesity comprising a soybean germinated embryo extract or a fraction thereof.
The term "improvement" of the present invention means all the actions in which the administration of the composition improves or alleviates obesity.
The term "food" of the present invention includes dairy products such as meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen and other noodles, gums, ice cream, various soups, drinks, tea, , A vitamin complex, a health functional food, and a health food, all of which include foods in a conventional sense.
The term "functional food" as used herein means the same term as "food for special health use" (FoSHU). In addition to nutrition, It means food. Here, the term "function" means that the structure and function of the human body have a beneficial effect for health use such as controlling nutrients or physiological action. The food of the present invention can be prepared by a method commonly used in the art and can be prepared by adding raw materials and ingredients which are conventionally added in the art. In addition, the formulations of the food can also be produced without restrictions as long as they are formulations recognized as food. The composition for food of the present invention can be manufactured in various forms, and unlike general pharmaceuticals, it has the advantage that there is no side effect that may occur when a drug is used for a long period of time, and is excellent in portability, Can be ingested as an adjuvant to promote the effect of preventing or improving obesity.
The health food refers to a food having an active health promotion or promotion effect compared with a general food, and a health supplement food refers to a food for health assistance. In some cases, the terms health functional foods, health foods, and health supplements are used.
Specifically, the health functional food is a food prepared by adding the extract of the present invention to food materials such as beverage, tea, spice, gum and confectionery, or by encapsulation, powdering, suspension or the like, But it has the advantage that there is no side effect that can occur when the food is used as a raw material and long-term use of the drug.
Since the food composition of the present invention can be routinely ingested, high efficacy against prevention or improvement of obesity can be expected, and thus it can be very usefully used.
The food composition may further comprise a physiologically acceptable carrier. The type of carrier is not particularly limited, and any carrier conventionally used in the art can be used.
In addition, the food composition may contain additional components that are commonly used in food compositions and can improve odor, taste, visual appearance, and the like. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid and the like. In addition, it may include minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu) It may also include amino acids such as lysine, tryptophan, cysteine, valine, and the like.
In addition, the food composition may further contain antiseptic agents (such as potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate), bactericides (Sodium nitrite), bleach (sodium sulfite), seasoning (sodium MSG glutamate, etc.), sweeteners (dicin, cyclamate, saccharin, etc.), coloring agents , Sodium, etc.), perfume (vanillin, lactones, etc.), swelling agents (alum, potassium hydrogen D-tartrate), emulsifiers, thickeners (foams), encapsulating agents, gum bases, foam inhibitors, solvents, And may include food additives. The additives may be selected and used in appropriate amounts depending on the type of food.
The extract of the present invention can be added intact or used together with other food or food ingredients, and can be suitably used according to conventional methods. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the food composition of the present invention may be added in an amount of not more than 50 parts by weight, specifically not more than 20 parts by weight, based on the food or beverage, when the food or drink is prepared. However, in case of long-term ingestion for health and hygiene purposes, the active ingredient may be contained in an amount not exceeding the above range and there is no problem in terms of safety.
As an example of the food composition of the present invention, it can be used as a health beverage composition. In this case, various flavors or natural carbohydrates can be added as an additional ingredient like ordinary beverages. The above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose, sucrose; Polysaccharides such as dextrin, cyclodextrin; Xylitol, sorbitol, erythritol, and the like. Sweeteners include natural sweeteners such as tau Martin and stevia extract; Synthetic sweetening agents such as saccharin and aspartame, and the like can be used. The ratio of the natural carbohydrate may be generally about 0.01 to 0.04 g, specifically about 0.02 to 0.03 g per 100 mL of the health beverage composition of the present invention.
In addition to the above, the health beverage composition may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid, salts of pectic acid, alginic acid, salts of alginic acid, organic acid, protective colloid thickener, pH adjuster, stabilizer, Alcohols or carbonating agents, and the like. It may also contain flesh for the production of natural fruit juices, fruit juice drinks, or vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health beverage composition of the present invention.
The food composition of the present invention may be contained in various weight percentages as long as it can exhibit the effect of preventing or improving obesity. Specifically, the extract of the present invention may be contained in an amount of 0.00001 to 100% by weight or 0.01 to 80% by weight, But are not limited thereto.
One aspect of the present invention provides a feed composition for preventing or ameliorating obesity comprising a soybean germinated embryo extract or a fraction thereof.
The term "feed " of the present invention means any natural or artificial diet, single meal, or the like ingredients for feeding, ingesting, digesting, or suitable for the animal.
The feed may comprise a feed additive or supplementary feed.
The kind of the feed is not particularly limited, and feeds conventionally used in the art can be used. Non-limiting examples of such feeds include vegetable feeds such as cereals, muscle roots, food processing busines logistics, algae, fibers, pharmaceutical buses, oils, fats, pastes or grain by-products; Animal feeds such as proteins, inorganic substances, fats, oils, fats, oils, monocellular proteins, animal plankton or foods. These may be used alone or in combination of two or more.
Since the soybean germinated embryo extract of the present invention inhibits the differentiation of adipocytes and reduces the size thereof and converts white adipocytes into brown adipocytes, it suppresses accumulation of unnecessary fat in vivo, and thus is useful for preventing or treating obesity .
1 is a graph showing the toxicity of soybean germinated embryo extract against lipogenic precursor cell line 3T3-L1. Soybean germinated embryo extracts were treated at concentrations of 50, 100, 200, 300, 400 and 500 ㎍ / mL.
Fig. 2 is a graph showing the effect of soybean germinated embryo extract on adipocyte differentiation. After induction of adipocyte differentiation, soybean germinated embryo extracts were prepared at the concentrations of 50, 100, 200, 300, 400 and 500 / / mL and treated with the cells. As a positive control, 100 μM of CLA (conjugated linoleic acid ). Control refers to non-differentiated control adipocytes, and control + insulin refers to differentiated control adipocytes. Soybean germ extract was prepared at a concentration of 500 μg / mL and treated with the cells.
FIG. 3 shows the effect of soybean germinated embryo extract (SA) and soybean germination extract (SG) on gene expression associated with adipocyte differentiation, wherein A is a Western blot image showing the amount of gene expression, B is the expression amount The results are shown in FIG. PPARγ, SREBP-1c and CEBPα were genes promoting the differentiation of adipocytes and Actin gene was used as a control group for comparing the expression levels of the genes.
FIG. 4 shows the effect of soybean germinated embryo extract on adipocyte size, A is an image showing the size of fat cells, and B is a graph showing the results of measuring the size. Obesity-induced mouse (OVX) was used as a negative control group by surgically removing ovaries. The mice were administered with 0.1 mg / kg, 1 mg / kg and 5 mg / kg of soybean germinated embryo extract, Respectively.
FIG. 5 shows the effect of soybean germinated embryo extract on white adipocytes and brown adipocytes. FIG. 5A shows the size of white adipocytes and FIG. 5B shows the number of brown adipocytes relative to the number of white adipocytes This is a graph showing the results. Obesity-induced mouse (OVX) was used as a negative control group by surgically removing ovaries. The mice were administered with 0.1 mg / kg, 1 mg / kg and 5 mg / kg of soybean germinated embryo extract, Respectively.
Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.
Example 1. Beans Germination embryo Evaluation of cytotoxicity of extracts
In order to prepare the soybean germinated embryo extract, the soybean germinated embryo prepared according to the previous study of the present inventor was used. Specifically, the soybean embryo was immersed in running water and germinated. After about 24 hours, the soybean germinated embryo was harvested, lyophilized and powdered to obtain a soybean germinated embryo powder. The resulting soybean germinated embryo powder was extracted by adding a fermented syrup, followed by filtration to obtain a liquid component, and the liquid component was lyophilized to obtain a soybean germana extract.
Then, in order to confirm the safety of the soybean germinated embryo extract, cytotoxicity of the extract to the cells was evaluated. Specifically, the lipogenic precursor cell line 3T3-L1 was subcultured in a 96-well plate at a density of 2 × 10 2 cells / well, and seed extracts of 50, 100, 200, 300, 400 and 500 μg / And cultured for 24 hours or 72 hours. After the completion of the incubation, the water-insoluble formazan formed by the addition of the MTT solution was dissolved in a dimethyl sulfoxide solution, and the optical density (OD value) of the dissolved formazan at 595 nm was measured. The relative cell viability (%) was assessed by measurement.
As a result, as shown in Fig. 1, it was confirmed that the soybean germinated embryo extract did not show cytotoxicity at all concentrations. As a result, the soybean germinated embryo extract of the present invention was found to be a safe substance free from harm to human body.
Example 2. Soybean for fat cell differentiation Germination embryo Analysis of the effect of extracts
Example 2-1. bean Germination embryo Analysis of lipid-lowering activity of extracts
To examine the effect of the extract on obesity treatment, the effect of soybean germinated embryo extract on the degree of adipocyte differentiation was analyzed.
3T3-L1 cells (Korean Cell Line Bank, Korea) were purchased from a fetal bovine serum (FBS) and 1% antibiotics (100 U / ml penicillin and 0.1 mg / mL streptomycin) ) In DMEM (Dulbecco's modified eagle medium) medium at 37 ° C under 5% CO 2 (95% air).
The cultured 3T3-L1 cells were treated with soybean germinated embryo extracts at a concentration of 50, 100, 200, 300, 400 and 500 / / mL, and the differentiation inducers (FBS, IBMX, dexanmethasone and Insulin) for 72 hours to differentiate into adipocytes. At this time, adipocyte without any substance was used as a control group and adipocyte treated with the same concentration of CLA (conjugated linoleic acid) was used as a comparative group.
As a result, as shown in FIG. 2, differentially treated control adipocytes (control + insulin) treated with insulin compared to control non-differentiated control fat cells (control) showed an increase in accumulated fat in the cells. In addition, it was confirmed that the soybean germinated embryo extract reduced the fat content of the differentiated adipocytes to the extent similar to that of CLA (conjugated linoleic acid) Was found to be better as the concentration of the extract increased. In addition, the soybean germinated embryo extract of the present invention was superior to the extract obtained by germinating the soybean itself, in that the fat cell differentiation inhibition effect was superior and the fat accumulation inhibition effect was also superior.
Example 2-2. Soy bean for fat cell differentiation Germination embryo Analysis of the effect of extracts
In order to analyze the effect of soybean germinated embryo extract on differentiation of adipocytes, the effect of the extract on the expression of biomarkers associated with adipocyte differentiation was examined.
Specifically, the degree of protein expression of PPARγ, SREBP-1c and CEBPα, genes promoting differentiation of adipocytes, was confirmed by Western blotting.
As a result, as shown in FIG. 3, the expression of PPARγ, SREBP-1c and CEBPα was decreased in the experimental group treated with the extract, as compared with control cells treated with DMSO. These results indicate that the soybean germinated embryo extract can inhibit fat cells from accumulating fat by inhibiting differentiation of adipocytes. In addition, when the soybean germinated embryo extract of the present invention was compared with the extract (SG) obtained by germination of the soybean itself, it was confirmed that the gene expression suppressing effect was superior and the fat accumulation inhibiting effect was also superior .
Example 3. Beans for fat cells in vivo Germination embryo Analysis of the effect of extracts
Example 3-1. Soybeans for fat cell size Germination embryo Analysis of the effect of extracts
In order to evaluate the effect of the extract on obesity, the effect of soybean germinated embryo extract on the fat cell size of obesity - induced animal models was analyzed by surgically removing ovaries. After menopause, as the amount of hormone secretion decreases, the basal metabolic rate decreases, and as fat decomposition decreases, the likelihood of obesity increases.
Specifically, the ovaries were removed from the ovaries by using 8-week-old female C57BL / 6 mice. For ovariectomy, a 1-2 cm skin incision was made in the lower middle region of the abdomen and forceps were applied to the ovaries (ovary) and oviduct (oviduct). One week after ovariectomy, randomized grouping was divided into randomized groupings using body weight measurement. Sonde was orally administered with 0.1, 1 and 5 mg / kg of soybean germinated embryo extracts six times a week.
After the end of the administration, the adipose tissue surrounding adipose tissue was removed, fixed with 10% formalin, embedded in paraffin, and the adipose tissue was sectioned to 4 μm. Then, the specimens were stained with HE (Hematoxylin and Eosin) staining. Observation of adipocytes was performed at a magnification of 400 × under a microscope. The lengths of long and short axes were measured for 10 cells in one field of view. The mean value of the long axis and short axis observed at 3 fields per individual was calculated and the average value of the groups was calculated.
As a result, as shown in FIG. 4, it was confirmed that the short axis and the long axis length of the adipocyte were increased in the negative control group (OVX) caused by menopause due to obesity as compared with the normal control group (con). On the other hand, it was confirmed that the diameter of the adipocyte was decreased in the test group administered with the extract, and the higher the concentration of the extract was, the higher the concentration of the extract (5 mg / kg / day) In the experimental group, the size of adipocytes was decreased to a level similar to that of the normal control group.
Example 3-2. Beans for white adipocytes and brown fat cells Germination embryo Analysis of the effect of extracts
Since white adipocytes are responsible for the development of adult diseases such as diabetes or obesity, brown adipocytes play a role in preventing obesity by burning fat as energy. Therefore, in order to evaluate the therapeutic effect of the extract on obesity, The effect of ovariectomy on white adipocytes or brown adipose cells in obesity - induced animal models was analyzed.
Specifically, the soybean germinated embryo extract was administered to an obesity-inducing animal model according to the method of Example 3-1, and the number of white adipocytes and brown adipocytes of the animal were examined. Immunohistochemistry using fatty acid oxidation-related UCP1 (mitochondrial uncoupling protein 1) protein was performed on adipose tissue cells, and brown adipocytes (UCP1) showing positive UCP1 response at three fields per subject at a microscope magnification of 200 times Were measured using LAS image analysis software.
As a result, as shown in FIG. 5, it was confirmed that the negative control group (OVX) and the normal control group (con) caused by obesity caused by menopause had fewer cells showing UCP1 positive reaction around and inside gonadal fat . On the other hand, in the test group administered with the extract, the number of UCP1-positive cells was increased compared to the negative and normal control groups, and at the same time, the size of white adipocytes decreased. This effect was better as the concentration of the extract was higher, and the number of brown adipocytes increased about 3.2 times in the experimental group administered with the high concentration of the extract (5 mg / kg / day) Respectively.
From the above results, it was found that the soybean germinated embryo extract showed a decrease in the size of white adipocytes and saturating the cells with brown adipocytes, thereby suppressing accumulation of adipocytes.
From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.
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| PCT/KR2016/002069 WO2017082479A1 (en) | 2015-11-09 | 2016-03-02 | Pharmaceutical composition for prevention or treatment of obesity comprising bean germinated embryo extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2005145826A (en) * | 2003-11-11 | 2005-06-09 | Kazuhiro Kotani | Composition containing sprouted bean for prevention and/or treatment of lifestyle disease |
| KR100706279B1 (en) * | 2005-11-11 | 2007-04-12 | (주)아모레퍼시픽 | Medicine and food compositions for improvement of obesity |
| KR101640258B1 (en) * | 2010-07-05 | 2016-07-15 | 주식회사 엘지생활건강 | Anti-obesity composition comprising red grape extracts, green tea extracts, soybean extracts, and L-carnitine |
| KR102200014B1 (en) * | 2013-05-06 | 2021-01-08 | (주)아모레퍼시픽 | Composition for preventing and treating climacteric symptoms comprising extract of bean containing coumestrol |
| KR20140141871A (en) * | 2013-05-31 | 2014-12-11 | 고려대학교 산학협력단 | Composition comprising germinated soybean peptides for anti-obesity |
-
2015
- 2015-11-09 KR KR1020150156867A patent/KR101749967B1/en active IP Right Grant
-
2016
- 2016-03-02 WO PCT/KR2016/002069 patent/WO2017082479A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017082479A1 (en) | 2017-05-18 |
| KR101749967B1 (en) | 2017-06-23 |
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