KR20170033681A

KR20170033681A - A method of algal phase separation

Info

Publication number: KR20170033681A
Application number: KR1020150131735A
Authority: KR
Inventors: 김종덕
Original assignee: 전남대학교산학협력단
Priority date: 2015-09-17
Filing date: 2015-09-17
Publication date: 2017-03-27
Also published as: KR101801743B1

Abstract

The present invention relates to a method for predicting the composition of algae, and more particularly, to a method for algae phase separation. The phase separation method of the present invention includes an alternating current applying step of applying an alternating current to water containing algae.

Description

A method of algal phase separation

The present invention relates to a pretreatment method for extracting constituent substances of algae, and more particularly to a method for algae phase separation.

Due to the drought and the influx of pollutants in the summer, there is a phenomenon of algal blooms in which the algae, including green algae, breed in the main rivers, causing serious problems such as the cause of fish and the difficulty of using them as drinking water in the vicinity of the water source It is becoming an economic problem. In order to deal with these green algae, the local governments are spreading them using water cannons mainly in the areas with severe green algae, or are carrying out the level of control activities using yellow loam. Even if these algae are collected, the collected algae are burned as organic wastes and buried.

However, green algae contains a large amount of carbon and lipid components, and when it is recycled, bioethanol and biodiesel can be produced, and by-products such as green algae produced during the production of these biofuels can be used as feed for livestock .

Therefore, in order to produce biofuels such as bioethanol using these algae, it is essential to develop a pretreatment process that economically recovers and crushes algae. With regard to the recovery of the algae, bioassay using bacteria such as chemical precipitation using chemical agents such as CaCl ₂ and FeCl ₂ (Kim et al ., Bioresource Technol ., 102: 3163-3168, 2011), Paenibacillus polymyxa (Bioflocculation, Oh et al ., Biotechnol. Lett ., 23: 1229-1234, 2001), eletcrocoagulation-electroflocculation, Valero et al ., Bioresource Technol. , 2015) have been reported.

However, chemical precipitation is costly due to the use of chemicals, requires additional procedures for post-treatment of water due to residual chemicals, and bioaggregation using bacteria also leads to increased costs due to the use of bacteria, In the case of the electric separation method using DC, there is a problem in that it is not a perfect phase separation, it takes a long time, and phase separation occurs only in a short distance between the electrodes, so that commercialization is difficult.

SUMMARY OF THE INVENTION It is an object of the present invention to provide a more economical and efficient method for separating algae particles. However, these problems are exemplary and do not limit the scope of the present invention.

According to one aspect of the present invention, there is provided a method for phase separation of algae including an AC current applying step of applying an AC current to water containing algae.

In the above method, the algae may be green algae, cyanobacteria, axal algae, euglena, brown algae, sulfur brown algae, sulfur algae or red algae.

In the above method, the water containing the algae may be water or an algal culture liquid contaminated with algae.

In the method, the alternating current may be a household alternating current having a voltage between 100 and 240 volts.

In this method, the AC current application step may be performed for 3 minutes to 1 hour.

The method according to one embodiment of the present invention as described above can be very useful for extracting useful components for bio-energy and the like from separated algae by separating algae and water from water containing algae by solid-liquid phase separation. Of course, the scope of the present invention is not limited by these effects.

FIG. 1 is a photograph of an AC AC applied to water containing algae according to an embodiment of the present invention and showing a state of solid phase separation according to the passage of time. FIG.
FIG. 2 is a photograph showing results of solid-liquid phase separation using various methods of algae according to an embodiment of the present invention.
Fig. 3 is a series of photographs of a phase change picture taken with DC current applied to the water containing algae (1 minute interval) with passage of time.

DETAILED DESCRIPTION OF THE INVENTION

In this method, the algae may be microalgae, and the microalgae may be selected from the group consisting of Cyanobacteria, Cryptophyta, Chrysophyta, Euglenophyta, Bacillariophyta, Phophophyta, Rholophyta, Chlorophyta, Charophyta, or a mixture of two or more thereof.

In the above method, the green algae are selected from the group consisting of Chlamydomonas sp., Botryococcus sp., Schizochytrium sp., Scenedesmus sp. in (Prymnesium sp.), cancer PD nium in (Amphidinium sp.), co Ella Sturm in (Coelastrum sp.), tetra-cell misses in (Tetraselmis sp.), tetracycline seutiseu in (Tetracystis sp.), prototype Chiffon Protosiphion sp., Hydrodictyon sp. Pediastrum sp., Chlorococcum sp., Ulothrix sp., Oedogonium sp. Oedogonium sp.), chlorella genus (chlorella sp.), styryl to Eau Ronnie help in (Stigeoclonium sp.), Fritz sh Ella in (Fritschiella sp.), spiro rep in (Spirogyra sp.), jig nematic in (Zygnema sp ) Or the genus Cladophora ( Cladophora sp.).

In the above method, the cyanobacteria are selected from the group consisting of Microcystis sp., Nostoc sp., Tolypothrix sp., Aulosira sp., Annabena sp. Anabaena sp., Planktothrix sp., Cylindrospermum sp., Fischerella sp., Gloeotrichia sp., Nordura sp. Nodularia sp., Oscillatoria sp., Aphanizomenon sp., Lyngbya sp., Rhaphidiopsis sp., Chrysosporum sp. Chrysosporum sp., Cuspidothrix sp., Synecoccus sp., Cylindrospermopsis sp., Dolichospermum sp., Porphyra spp. Medium speed (Phormidium sp.), in Tycho nematic (Tychonema sp.), woro niche in California (Woronichinia sp.) or Trojan are in Spira (Arthr ospira sp.).

In the method, the alternating current may be a household alternating current having a voltage between 100 and 240 volts. However, if the total volume of contaminated water in the culture or microalgae is increased, the applied alternating voltage may be increased through boosting.

In this method, the AC current application step may be performed for 3 minutes to 1 hour. The application time may be adjusted depending on the total volume of contaminated water of the culture medium or microalgae.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. It should be understood, however, that the invention is not limited to the disclosed embodiments, but may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, Is provided to fully inform the user.

Example

Example 1: Phase separation of cultured algae

The algae used for the phase separation experiments was Microcystis aeruginos , which is the main cause of the algae phenomenon. First, a phase change was observed for 5 minutes while applying a current of 100 to 240 V (5 A) for domestic use to 5 liters of microsystic erythromycin culture medium. The application of the household AC was performed by inserting a plug exposed to the electric wire into a household AC outlet and inserting the exposed wire into a beaker containing the culture liquid without using a special power supply, In order to prevent such a problem, a partition wall is installed in a beaker and both wires are inserted between the partition walls.

As a result of the experiment, algae flocculated on the top of the beaker over time, and the culture liquid at the bottom became gradually transparent (Fig. 1).

Example 2:

Then, the present inventors carried out the same experiment as that of Example 1 in order to differentiate the microalgae phase separation method according to the embodiment of the present invention from other kinds of microalgae (purchased from a microalgae bank).

Specifically, the culture broth of Chlrolella vulgaris , Botryococcus braunii , Chlamidomonas reinharditi , and Arthrospira platensis was poured into a 12 L bottle, And a household alternating current of 100 to 240 V was applied for 30 minutes.

As a result, as shown in FIG. 2, it can be observed that all microalgae, regardless of green algae and blue algae, flocculate on the top of the beaker by application of alternating current.

As shown in the above results, the bird-phase phase separation method according to an embodiment of the present invention can be applied to a microalgae culture medium or micro-algae contaminated water without using any chemical agent and without using a special apparatus, It was confirmed that the microalgae could be separated into a liquid phase and an organic phase efficiently. The separated microalgae can be used as a raw material for the production of biodiesel or bioethanol, and the separated water can be discharged to the river or lake again without any additional treatment or with minimal post treatment. Therefore, the method of the present invention can be used as a very environmentally friendly and economical microalgae separation method.

Comparative Example: Separation of microalgae using DC

The inventors of the present invention have found that, in order to confirm whether or not floating micro-algae can be removed even by using direct current, the direct current of 100 V, 5 A was used in place of the alternate current used in Examples 1 and 2, In the same manner, a direct current of 100 V (5 A) was applied to the culture solution of Microcystis aeruginos in a 5 L beaker and the phase change was observed for 5 minutes (FIG. 3).

As a result, as shown in Fig. 3, floating aggregation of microalgae was not observed at all when DC current was applied. In addition, in the case of the conventional method of floating micro-algae flocculation removing method, there is a disadvantage in that an electrode using a special metal such as aluminum is used, and when the distance between electrodes is shortened, the efficiency is drastically decreased. Able to know. On the other hand, it can be seen that the AC current applying method according to an embodiment of the present invention can be very useful for purifying water contaminated with green algae by flocculating and flocculating microalgae quickly without using special chemicals.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

Claims

A method for phase separation of algae, comprising applying an alternating current to water containing algae.

The method according to claim 1,
Wherein the alga is a green alga, a cyanobacteria, an axal alga, a euglena, a brown alga, a yellow algae, a sulfur bird, or a red algae.

The method according to claim 1,
Wherein the water containing the alga is a water or algal culture contaminated with algae.

The method according to claim 1,
Wherein the voltage of the alternating current is 100 to 240 volts.

The method according to claim 1,
Wherein the step of applying the alternating current is performed for 3 minutes to 1 hour.

3. The method of claim 2,
The green alga may be selected from the group consisting of Chlamydomonas sp., Botryococcus sp., Schizochytrium sp., Scenedesmus sp., Prymnesium sp. ), cancer PD nium in (Amphidinium sp.), co Ella Sturm in (Coelastrum sp.), tetra-cell misses in (Tetraselmis sp.), in tetracycline seutiseu (Tetracystis sp.), prototype chiffon in (Protosiphion sp. ), Hydrodictyon sp., Pediastrum sp., Chlorococcum sp., Ulothrix sp., Oedogonium sp., Pseudomonas sp. chlorella genus (chlorella sp.), styryl to Eau Ronnie help in (Stigeoclonium sp.), Fritz sh Ella in (Fritschiella sp.), spiro rep in (Spirogyra sp.), jig nematic in (Zygnema sp.) or climb also Cladophora sp., Phase separation method.

3. The method of claim 2,
The blue-green algae are micro during seutiseu in (Microcystis sp.), No stock in (Nostoc sp.), Tall report Riggs in (Tolypothrix sp.), OLLO Shirakawa in (Aulosira sp.), Ana vena in (Anabaena sp.), Planck Saturday matrix in (Planktothrix sp.), cylinder de los percha drought in (Cylindrospermum sp.), piswe mozzarella in (Fischerella sp.), in Autry teeth glow (Gloeotrichia sp.), Notre dulra Ria in (Nodularia sp , Oscillatoria sp., Aphanizomenon sp., Lyngbya sp., Rhaphidiopsis sp., Chrysosporum sp., Chrysosporum sp. Such as Cuspidothrix sp., Synecoccus sp., Cylindrospermopsis sp., Dolichospermum sp., Phormidium sp. Sp. ), Tichonema sp., Woronichinia sp., Or Arthrospira sp. method.