KR20170012687A - Device for microfluidic - Google Patents

Device for microfluidic Download PDF

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Publication number
KR20170012687A
KR20170012687A KR1020150103746A KR20150103746A KR20170012687A KR 20170012687 A KR20170012687 A KR 20170012687A KR 1020150103746 A KR1020150103746 A KR 1020150103746A KR 20150103746 A KR20150103746 A KR 20150103746A KR 20170012687 A KR20170012687 A KR 20170012687A
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KR
South Korea
Prior art keywords
speed
porous substrate
microfluidic device
developing solution
barrier
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KR1020150103746A
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Korean (ko)
Inventor
이진기
임호섭
투라압 자프리 알리
Original Assignee
성균관대학교산학협력단
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Priority to KR1020150103746A priority Critical patent/KR20170012687A/en
Publication of KR20170012687A publication Critical patent/KR20170012687A/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

The present invention relates to a microfluidic device. According to the present invention, the microfluidic device comprises: a porous substrate in which a developing solution is developed; and a speed adjustment unit formed on a developing path in which the developing solution is developed on the porous substrate. To this end, the microfluidic device can control transfer speed of a fluid developed on the porous substrate.

Description

DEVICE FOR MICROFLUIDIC < RTI ID = 0.0 >

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a microfluidic device, and more particularly, to a microfluidic device capable of controlling a moving speed of a developing solution.

Analysis of biological fluids is useful for monitoring the health status of individuals or populations and for diagnosing diseases or conditions. In general, diagnostic assays not only require large, costly laboratory equipment that must be performed by skilled personnel, but also require a significant amount of biological samples.

However, medical diagnosis costs are becoming a serious problem as the age of the elderly increases and the proportion of older age groups increases. In addition, most current diagnostic assays are not useful in emergency situations or home health care (home care) situations.

In order to solve this problem, it is necessary to have a low-cost diagnosis apparatus capable of diagnosing disease at a low cost in a medical institution or a home, and therefore, it is possible to operate only a small amount of biological sample without inconvenience.

Recently, it is cheap, it does not require a separate pump due to the flow of fluid using the principle of capillary phenomenon, and it is easy to analyze chemical substances by applying colorimetric analysis method. Therefore, Application of microfluidic devices based on substrates has been attracting attention. A paper-based microfluidic device-based analysis device is called a microfluidic paper-based analytical device (μPAD) or paper-based microfluidic device.

1 is a schematic view illustrating an analysis process using a conventional paper-based microfluidic device. Referring to FIG. 1, when a fluid such as a developing solution D is dropped on a microfluidic device 10 where a sample S located at an end is located, the developing solution D spreads toward the sample S. That is, the developing liquid D moves toward the sample S along the capillary phenomenon, and reacts with the sample S to perform analysis.

However, there is a problem that the reaction time between the developing solution D and the sample S is insufficient when the moving speed of the developing solution D is too high.

When the temperature is raised, the reaction between the developing solution (D) and the sample (S) is promoted, but the velocity of the fluid is not affected, and development of a new medium for controlling the incubation time by controlling the velocity of the fluid is difficult There is a problem.

SUMMARY OF THE INVENTION Accordingly, it is an object of the present invention to provide a microfluidic device capable of controlling a moving speed of a fluid to be developed on a porous substrate by forming a speed regulating portion on a porous substrate.

Another object of the present invention is to provide a microfluidic device capable of increasing the flow path of a fluid to be developed on a porous substrate.

The present invention also provides a microfluidic device capable of controlling the moving speed of the fluid and the moving path of the fluid without being limited to the type of the developing fluid, because the material of the barrier and the speed adjusting portion can be changed depending on the nature of the developing fluid.

This object is achieved according to the present invention by providing a porous substrate on which a developing solution is developed; And a speed regulator formed on the development path where the development liquid is developed in the porous substrate.

Here, it is preferable that the speed regulator is formed of a material that does not absorb the developing solution.

Here, it is preferable that a plurality of the speed adjusting units are spaced apart from each other.

Here, the moving speed of the developing solution is preferably controlled by the number per unit area of the speed adjusting part.

Here, the moving speed of the developing solution may be controlled by the size of the speed adjusting part.

Here, the porous substrate is preferably made of paper, fiber, non-woven fabric, or porous metal.

Here, it is preferable to further include a barrier formed on the porous substrate to form a flow path which is a development path of the developing solution.

Here, the barrier is preferably made of a material that does not absorb the developing solution.

According to the present invention, there is provided a microfluidic device capable of controlling the moving speed of a fluid to be developed on a porous substrate.

In addition, according to the present invention, various kinds of speed controllers can be used, so that speed control of a developing solution can be made according to the situation.

Further, the speed of the developing solution can be easily controlled by easily adjusting the density, size and arrangement of the speed adjusting portion.

Further, since the material of the speed adjusting section can be changed according to the nature of the developing solution, it can be applied without being limited to the type of developing solution.

In addition, since the barrier and the speed adjusting portion can be formed at the same time, the manufacturing is easy and the manufacturing time is short.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view illustrating an analysis process using a conventional paper-based microfluidic device,
2 is a schematic front view of a microfluidic device according to an embodiment of the present invention,
Figure 3 is a schematic perspective view of the microfluidic device of Figure 2,
FIG. 4 is a photograph of a microfluidic device of FIG. 2,
5 is a view schematically showing a movement path of the developing solution of the microfluidic device of FIG. 2,
FIG. 6 is an experimental graph showing the presence or absence of the speed regulator of the microfluidic device of FIG. 2,
FIG. 7 shows various modifications of the size and density of the speed regulator of the microfluidic device of FIG. 2,
FIG. 8 is an experimental graph according to a modification of FIG. 7,
Fig. 9 shows various modifications of the arrangement of the microfluidic device of Fig. 2,
10 is an experimental graph according to a modification of Fig. 8,
11 is a photolithography method of the microfluidic device of FIG. 2,
FIG. 12 shows a manufacturing method of the microfluidic device of FIG. 2 by wax coating.

Hereinafter, a microfluidic device according to an embodiment of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 2 is a schematic front view of a microfluidic device according to an embodiment of the present invention, FIG. 3 is a schematic perspective view of the microfluidic device of FIG. 2, and FIG. 4 is a photograph of a microfluidic device of FIG. 2 to 4, a microfluidic device 100 according to an embodiment of the present invention includes a porous substrate 110, a barrier 120 formed on the porous substrate 110, And a speed regulating unit 130 formed on the motor.

The porous substrate 110 constitutes the base of the microfluidic device 100 according to the present invention. The porous substrate 110 may be formed of paper, but not limited to, a paper, a porous material such as a nonwoven fabric, a porous metal, or the like. When the porous substrate 110 is made of paper, the paper is a polymer of fiber bundles composed of cellulose fibers, and since there are numerous pores and the capillary phenomenon easily occurs, liquid is permeated into the hydrophilic fibers by the capillary force without any device , And properties such as hydrophilicity and chemical reactivity can be changed very easily. Further, if the porous substrate 110 is made of paper, it may be provided with chromatography paper. 3, the microfluidic device 100 is slightly exaggerated, but the actual thickness of the porous substrate 110 is about 200 μm or less.

That is, the porous substrate 110 is a region where a fluid such as a developing solution D is prepared by a material such as paper or the like and developed by a capillary phenomenon. Herein, the fluid is absorbed by the porous substrate 110, As shown in FIG.

The barrier 120 is a structure for forming a flow path 111 through which the development liquid D moves on the porous substrate 110. The barrier 120 is made of a material that does not absorb the developing liquid D, and in this embodiment, the barrier 120 is made of a hydrophobic material.

In this embodiment, the barrier 120 is formed through the thickness of the porous substrate 110. 3, since the barrier 120 is formed by being absorbed by the porous substrate 110, the barrier 120 is hardly protruded from the upper surface of the porous substrate 110 in practice.

A boundary is formed by the barrier 120 which is made of a material which does not absorb fluid such as the developing liquid D and the developing liquid D is developed along the flow path 111 formed inside the boundary.

The speed adjusting unit 130 is a structure for adjusting the developing speed, that is, the moving speed, of the developing liquid D spreading along the flow path on the porous substrate 110. The speed regulator 130 is made of a material which does not absorb the developing liquid D like the barrier 120 and is made of a hydrophobic material in this embodiment. The speed regulator 130 is provided to have a width smaller than the width of the flow path 111, and a plurality of the speed regulator 130 are spaced apart from each other.

In this embodiment, the barrier 120 and the velocity regulator 130 are made of a hydrophobic material. However, it is obvious that the barrier 120 and the velocity regulator 130 may be made of a hydrophobic material. That is, the material is not limited unless the developing liquid D is absorbed.

Fig. 5 is a schematic view showing the movement path of the developing solution of the microfluidic device of Fig. 2; Referring to FIG. 5, the speed adjusting unit 130 acts as a resistance to the developing liquid D and increases the moving path of the developing liquid D, thereby slowing the moving speed of the developing liquid D. In the absence of the speed regulating unit 130, the developing liquid D is developed without resistance. However, if the speed regulating unit 130 is provided, And the velocity of the liquid D is slowed by the speed control unit 130 because the movement path becomes a curve rather than a straight line and the movement path itself increases.

Comparative Example

The time required for the development liquid D to travel a predetermined distance was measured in the case where the speed regulating unit 130 was formed on the flow path 111 and the case where the speed regulating unit 130 was not formed.

FIG. 6 is an experimental graph showing the presence or absence of the speed regulator of the microfluidic device of FIG. Referring to FIG. 6, it takes about 2200 seconds to move 15 mm when the speed regulator 130 is formed. When the speed regulator 130 is not formed, it takes about 1300 seconds to move 15 mm, It can be seen that the time required to travel the same distance is longer than when the speed controller 130 is not provided.

Experimental Example  One

The time required for the developing solution D to travel a predetermined distance was measured by adjusting the number of the speed adjusting portions 130 per unit area of the flow path 111. [

FIG. 7 shows various modified examples according to the size and density of the speed regulator of the microfluidic device of FIG. 2, and FIG. 8 is an experimental graph according to the modification of FIG.

8, when the distance between the speed adjusting portions 130 is 1.5 mm and the number of the speed adjusting portions 130 per unit area is minimized, that is, when the density of the speed adjusting portion 130 is the lowest, It took about 2200 seconds to travel. When the interval between the speed regulators 130 was 1.15 mm, the time required was about 2,400 seconds. When the interval between the speed regulators 130 was 0.65 mm, it took about 3000 seconds. Accordingly, it can be seen that the more the number of unit area speed regulators 130 is, the longer the time required to travel the same distance.

Experimental Example  2

The time required for the developing liquid D to travel a predetermined distance was measured by varying the size and arrangement of the speed regulating unit 130.

FIG. 9 shows various modified examples according to the arrangement of the microfluidic device of FIG. 2, and FIG. 10 is an experimental graph according to the modified example of FIG.

Referring to FIG. 10, when the velocity regulator 130 is not formed, the time required for the development liquid D to travel 30 mm is about 3200 seconds.

Next, when the diameter of the speed regulating part 130 is 0.3 mm, it takes about 5700 seconds for the developing liquid D to travel 30 mm when it is arranged to be offset from the neighboring speed regulating part 130, The time taken for the developing liquid D to move 30 mm when the developing liquid D is arranged in parallel with the speed adjusting unit 130 is about 5300 seconds.

When the diameter of the speed regulating part 130 is set to 0.5 mm and it is arranged to be offset from the adjacent speed regulating part 130, the time taken for the developing liquid D to travel 30 mm is about 6700 seconds, The time taken for the developing liquid D to move 30 mm when the developing liquid D is arranged in parallel with the adjusting unit 130 is about 5900 seconds.

Therefore, in the case of forming the speed adjusting portion 130 of the same size, the moving speeds of the adjacent speed adjusting portions 130 are slower than those of the adjacent speed adjusting portions 130 arranged side by side, It can be seen that the moving speed is slower when the size of the recording medium 130 is larger.

In summary, the speed of the developing liquid D is increased by the speed adjusting unit 130, and the speed adjusting unit 130 adjusts the size, arrangement and density of the developing liquid D so that the developing speed of the developing liquid D is precisely adjusted Can be controlled.

Next, a method of manufacturing a microfluidic device according to an embodiment of the present invention will be described.

11 is a photolithographic method for manufacturing the microfluidic device of FIG.

Referring to FIGS. 11A and 11B, the porous substrate 110 is immersed in a photoresist P to sufficiently impregnate the porous substrate 110 with the photoresist P. The photoresist P is selectively removed as a light-receiving portion and a non-light-receiving portion in a subsequent developing process using the characteristic that the solubility of the developing solution is changed by receiving light of a specific wavelength.

Since the photoresist P has a hydrophobic property, the barrier 120 and the velocity regulator 130 can be formed by the mask M using the photoresist.

Thereafter, the organic solvent remaining in the photoresist P is removed, and the mask M is aligned as shown in Fig. 11 (c). As shown in FIG. 11 (d), exposure under ultraviolet light forms a flow path 111, which is a hydrophilic channel in which the developing liquid D spreads, and a barrier 120 and a speed adjusting part 130, which are hydrophobic parts .

Since the barrier 120 and the velocity adjusting unit 130 can form a shape and a region corresponding to the patterning of the mask M, the flow path 111 and the velocity adjusting unit 130 can be formed at the same time.

FIG. 12 shows a manufacturing method of the microfluidic device of FIG. 2 by wax coating.

12A and 12B, the wax W is coated on the porous substrate 110 using a wax printer in a shape corresponding to the barrier 120 and the speed adjusting part 130. 12 (c), when the porous substrate 110 coated with the wax W is heated, the wax W is melted and penetrated into the porous substrate 110, and the wax W is developed Since the wax W does not absorb the liquid D, the wax W impregnated therein becomes the barrier 120 and the speed adjusting part 130.

Since the barrier 120 and the speed adjusting unit 130 are formed by the wax W impregnated into the porous substrate 110, the flow channel 111 and the speed adjusting unit 130 can be formed at the same time.

Thus, according to the present invention, there is provided a microfluidic device capable of controlling the rate of movement of a fluid developed on a porous substrate.

The scope of the present invention is not limited to the above-described embodiments, but may be embodied in various forms of embodiments within the scope of the appended claims. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the present invention as defined by the appended claims.

100: Microfluidic device 110: Porous substrate
120: barrier 130: speed control unit

Claims (8)

A porous substrate on which a developing solution is developed; And
And a velocity regulator formed on the development path in which the development liquid is developed in the porous base material. The method according to claim 1,
Wherein the speed regulator is made of a material that does not absorb the developing solution. The method according to claim 1,
Wherein a plurality of the speed adjusting portions are spaced apart from each other. The method of claim 3,
Wherein the moving speed of the developing solution is controlled by the number per unit area of the speed adjusting part. The method of claim 3,
Wherein the moving speed of the developing solution is controlled by the size of the speed adjusting part. The method according to claim 1,
Wherein the porous substrate is made of paper, fiber, non-woven fabric, or porous metal. 7. The method according to any one of claims 1 to 6,
Further comprising a barrier formed on the porous substrate to form a flow path as a development path of the development liquid. 8. The method of claim 7,
Wherein the barrier is made of a material that does not absorb the developing solution.
KR1020150103746A 2015-07-22 2015-07-22 Device for microfluidic KR20170012687A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190058249A (en) * 2017-11-20 2019-05-29 주식회사 엘지화학 A device and method for qualitative and quantitative analysis of heavy metals using a rotary disc system

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190058249A (en) * 2017-11-20 2019-05-29 주식회사 엘지화학 A device and method for qualitative and quantitative analysis of heavy metals using a rotary disc system
KR20190058248A (en) * 2017-11-20 2019-05-29 주식회사 엘지화학 A device and method for qualitative and quantitative analysis of heavy metals using a rotary disc system
KR20190058247A (en) * 2017-11-20 2019-05-29 주식회사 엘지화학 A device and method for qualitative and quantitative analysis of heavy metals using a rotary disc system

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