KR20170005699A - Microalgae having exhaust gas tolerance and method for culuturing thereof - Google Patents

Abstract

The present invention relates to a technology for increasing lipid contents in microalgae via a method for injecting exhaust gas produced in thermoelectric power plants. More specifically, the present invention relates to a technology which involves the use of exhaust gas produced in thermoelectric power plants while culturing high concentration microalgae, in an attempt to increase lipid contents present in microalgae produced thereby. Additionally, reduction in an amount of carbon dioxide in the exhaust gas is possible as microalgae grow.

Description

TECHNICAL FIELD [0001] The present invention relates to a microalgae of exhaust gas resistance and a method of culturing microalgae,

Disclosed herein are novel microalgae, methods for culturing microalgae using the exhaust gas, methods for increasing the lipid content of microalgae, and methods for selecting microalgae with high lipid content.

The use of fossil fuels by highly industrialization has produced a large amount of greenhouse gases and has played a role in global warming. In addition, excessive use of fossil fuels leads to depletion of petroleum in the present economic society, and many researchers at home and abroad have developed alternative energy as a means of replacing fossil fuels.

Alternative energy should not be pollution-free and environmental pollution as well as replacement of fossil fuels. Alternative energy sources can include renewable energy and include solar, bio, wind, hydro, marine, waste, geothermal, fuel cells, and hydrogen. Bio-energy is a diverse range of raw materials and currently uses microalgae as a raw material for the third generation.

On the other hand, conventional microalgae are produced using commercial carbon dioxide as a raw material. In addition, the demand for microalgae containing useful substances is increasing.

Korean Patent Publication No. 10-2013-0091524

In one aspect of the present disclosure, there is provided a novel microalgae.

In another aspect of the present invention, there is also provided a method for culturing microalgae using an exhaust gas.

In another aspect of the present invention, there is provided a method for simultaneously cultivating microalgae and removing carbon dioxide from the exhaust gas.

In another aspect of the present disclosure, there is also provided a method for increasing the lipid content of microalgae.

In another aspect of the present invention, there is provided a method for selecting microalgae having an excellent lipid content.

One aspect of the present invention relates to a method for the treatment of acutodesmus sp . ) KGE 30 We want to provide microalgae.

One aspect of the present invention provides a method for simultaneously cultivating a microalgae and removing carbon dioxide from the exhaust gas, which comprises culturing a microalgae by supplying an exhaust gas containing carbon dioxide to the microalgae.

One aspect of the present invention provides a method for promoting the growth of microalgae, comprising culturing microalgae by supplying an exhaust gas containing carbon dioxide to the microalgae.

One aspect of the present invention provides a method for increasing lipid production in a microalgae, comprising culturing a microalgae by supplying an exhaust gas containing carbon dioxide to the microalgae.

An aspect of the present invention provides a method for increasing the production amount of fatty acids in a microalgae, comprising culturing a microalgae by supplying an exhaust gas containing carbon dioxide to the microalgae.

One aspect of the present invention is a method for screening microalgae that is superior in lipid productivity, the method comprising measuring one or more of the following: i) culturing microalgae using exhaust gas and commercial carbon dioxide, Measure; And ii) a method for measuring lipid content by culturing microalgae using exhaust gas and commercial carbon dioxide.

The method according to one aspect of the present invention can be expected to secure microbial biomass in a short time.

The method according to one aspect of the present invention can be applied without changing the existing facilities because the conventional carbon dioxide supplying microalgae culture apparatus can be used.

Microalgae productivity can be established at a rate equal to or higher than the rate of culturing using commercial carbon dioxide by the method according to one aspect of the present invention.

The method according to one aspect of the present invention has a cost saving effect on the supply of carbon dioxide.

The method according to one aspect of the present invention has the effect of increasing the lipid content of microalgae.

The microalgae produced by the method according to one aspect of the present invention can produce the biodiesel raw material with high productivity.

Since the method according to one aspect of the present invention uses a thermal power plant exhaust gas, the carbon dioxide present in the exhaust gas can be removed to reduce greenhouse gas emissions.

The method according to one aspect of the present invention can generate carbon dioxide sales revenue through carbon emission trading during carbon trading.

Figure 1 shows the microalgae Acudodesmus < RTI ID = 0.0 > obliquus ) KGE 30 in air, commercial carbon dioxide, and thermal power plant exhaust gas.
Fig. 2 is a schematic view of an Acutodesmus Obliquus ) This is a schematic diagram in which the maximum growth rate is increased by continuously injecting thermal power plant exhaust gas into KGE 30.
FIG. 3 shows a schematic diagram of an Acutodesmus obliquus ) KGE 30, the rate of carbon dioxide removal in a thermal power plant.

Bio-energy can be used as a raw material for biodiesel and has a very high yield per unit area compared to other raw materials. The advantages of using microalgae are as follows: 1) Microalgae can be cultivated using only sun, water, atmospheric carbon dioxide or anthropogenic carbon source. 2) Photosynthesis works to fix carbon dioxide, which is the main cause of global greenhouse gas, 3) First generation lignocellulosic second generation The production per unit area is superior to the biomass of sugar cane. 4) The production efficiency is excellent because the lipid content is very high (20 ~ 70%) compared to the dry weight of microalgae. Do.

In addition, the large amount of carbon dioxide present in the thermal power plant can promote the growth of microalgae, and the amount of lipid present in the microalgae can be increased by SOx, NOx, etc. known as toxic substances.

In addition, the produced microalgae can be used as a raw material for functional food such as omega 3, carotenoid, etc., and the content can be increased or the production amount can be increased by injecting carbon dioxide.

In one aspect of the present invention, Acutodesmus obliquus KGE 30 microalgae are provided. Specifically, the microalgae may be microalgae having the accession number KCTC18367P.

In one aspect of the present invention, there is provided a method for simultaneously cultivating a microalgae and removing carbon dioxide in an exhaust gas, which comprises culturing a microalgae by supplying an exhaust gas containing carbon dioxide to the microalgae.

In one aspect of the present invention, there is provided a method for promoting the growth of microalgae, comprising culturing microalgae by supplying an exhaust gas containing carbon dioxide to the microalgae.

In one aspect of the present invention, there is provided a method for increasing lipid production in a microalgae, comprising culturing a microalgae by supplying an exhaust gas containing carbon dioxide to the microalgae.

In one aspect of the present invention, there is provided a method for increasing the production amount of fatty acids in a microalgae, comprising culturing a microalgae by supplying an exhaust gas containing carbon dioxide to the microalgae.

In one aspect of the present invention, the fatty acid content is at least 1.00% (w / w), at least 1.05% (w / w), at least 1.10% (w / 1.25% (w / w), 1.30% (w / w), 1.35% (w / w), 1.40% (w / w / w or more, 1.50% w / w, 1.55% w / w, 1.60% w / w, 1.65% w / w, 1.70% (w / w), not less than 1.80% (w / w), not less than 1.90% (w / w), not less than 2.00% (w / 2.30% (w / w), 2.40% (w / w), 2.50% (w / w), 2.60% (w / w), 2.70% (w / (w / w), not less than 2.80% (w / w), not less than 2.85% (w / w), not less than 2.90% (w / w), not less than 2.95% (w / (W / w), 3.10% (w / w), 3.15% (w / w), 3.20% (w / w), 3.22% (w / w / w).

In one aspect of the present invention, the concentration of carbon dioxide in the exhaust gas is at least 0.1%, at least 0.2%, at least 0.3%, at least 0.4%, at least 0.5%, at least 0.6%, at least 0.7%, at least 0.8% More than 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12% , At least 14%, at least 15%, at least 16%, at least 17%, at least 18%, or at least 19%.

The carbon dioxide concentration of the exhaust gas is 80% or less, 70% or less, 60% or less, 50% or less, 40% or less, 30% or less, 29% or less, 28% or less, 27% or less, Or less, 24% or less, 23% or less, 22% or less, 21% or less, 20% or less, 19% or less, 18% or less, 17% or less, 16% or less, 15% or less, 14% or less, 13% , 12% or less, 11% or less, or 10% or less.

When the carbon dioxide concentration of the exhaust gas is within the above range, the growth of the microalgae is effectively promoted and the production amount of the lipid or fatty acid of the microalgae can be effectively increased.

In one aspect of the present invention, the exhaust gas is an exhaust gas from a thermal power plant.

In one aspect of the present invention, the exhaust gas is injected at each growth slowing point of the microalgae, and the growth slowing point of the microalgae is measured by measuring the OD value measured at 680 nm at 680 nm 1.24 times, 1.21 times, 1.21 times, 1.21 times, 1.21 times, 1.21 times, 1.19 times, 1.18 times, 1.17 times, 1.16 times, or 1.15 times the OD value And a time point of time when the first time point is reached.

When the ratio of the OD value is within the above range, the growth of the microalgae is slowed down. By injecting the exhaust gas at the time of the slowing, the growth of the microalgae is effectively promoted and the lipid, fatty acid or unsaturated fatty acid The production amount can be effectively increased.

In one aspect of the present invention, the microalgae may be microalgae having a growth rate of 35% to 95% or more in growth rate at an optimum pH in an environment of pH 3 to 7.

In one aspect of the present invention, the microalgae have a growth rate of 35% or more, a growth rate of 40% or more, a growth rate of 45% or more, a growth rate of 50% or more of the growth rate at the optimal pH (optimal pH) % Growth rate, more than 55% growth rate, more than 60% growth rate, more than 65% growth rate, more than 70% growth rate, more than 75% growth rate, more than 80% growth rate, more than 85% growth rate, more than 90% Characterized in that the microalgae are microalgae representing the microalgae.

In one aspect of the present invention, the microalgae have a growth rate of 35% or more, a growth rate of 40% or more, a growth rate of 45% or more, a growth rate of 50% or more of the growth rate at the optimum pH (optimal pH) % Growth rate, more than 55% growth rate, more than 60% growth rate, more than 65% growth rate, more than 70% growth rate, more than 75% growth rate, more than 80% growth rate, more than 85% growth rate, more than 90% Characterized in that the microalgae are microalgae representing the microalgae.

In one aspect of the present invention, the microalgae has a growth rate of 35% or more, a growth rate of 40% or more, a growth rate of 45% or more, a growth rate of 50% or more of the growth rate at the optimal pH (optimal pH) % Growth rate, more than 55% growth rate, more than 60% growth rate, more than 65% growth rate, more than 70% growth rate, more than 75% growth rate, more than 80% growth rate, more than 85% growth rate, more than 90% Characterized in that the microalgae are microalgae representing the microalgae.

In one aspect of the present invention, the microalgae have a growth rate of 35% or more, a growth rate of 40% or more, a growth rate of 45% or more, a growth rate of 50% or more of the growth rate at the optimal pH (optimal pH) % Growth rate, more than 55% growth rate, more than 60% growth rate, more than 65% growth rate, more than 70% growth rate, more than 75% growth rate, more than 80% growth rate, more than 85% growth rate, more than 90% Characterized in that the microalgae are microalgae representing the microalgae.

In one aspect of the present invention, the microalgae have a growth rate of 35% or more, a growth rate of 40% or more, a growth rate of 45% or more, a growth rate of 50% or more of the growth rate at the optimum pH (optimal pH) % Growth rate, more than 55% growth rate, more than 60% growth rate, more than 65% growth rate, more than 70% growth rate, more than 75% growth rate, more than 80% growth rate, more than 85% growth rate, more than 90% Characterized in that the microalgae are microalgae representing the microalgae.

In one aspect of the present invention, the microalgae have a growth rate of 35% or more, a growth rate of 40% or more, a growth rate of 45% or more, a growth rate of 50% or more of the growth rate at the optimal pH (optimal pH) % Growth rate, more than 55% growth rate, more than 60% growth rate, more than 65% growth rate, more than 70% growth rate, more than 75% growth rate, more than 80% growth rate, more than 85% growth rate, more than 90% Characterized in that the microalgae are microalgae representing the microalgae.

When the growth rate of the microalgae is within the above range, an excellent growth rate can be shown in the exhaust gas containing carbon dioxide as a carbon source.

Specifically, the microalgae are selected from the group consisting of Scenedesmus sp .), Neoplaselmis sp . ), ≪ / RTI > Micractinium sp . And Acutodesmus sp. ) . ≪ / RTI > More specifically, the microalgae are selected from the group consisting of Scenedesmus obliquus , Micractinium reisseri , and Acutodesmus obliquus . In the present invention,

In one aspect of the present invention, the microalgae is Acutodesmus obliquus KGE 30. Specifically, the microalgae may be microalgae having the accession number KCTC18367P.

In one aspect of the present invention, culturing the microalgae comprises culturing the microalgae by supplying wastewater together with the exhaust gas in addition to the exhaust gas.

On the other hand, the wastewater may be wastewater containing any one of livestock manure, mine drainage, domestic wastewater and food wastewater.

In one aspect of the present invention, there is provided a method for screening microalgae that is superior in lipid productivity, the method comprising measuring one or more of the following: i) culturing microalgae using exhaust gas and commercial carbon dioxide, Measurement of each growth; And ii) culturing microalgae using exhaust gas and commercial carbon dioxide, respectively, to provide a measure of the respective lipid content.

In one aspect of the invention, the ⅰ) or ⅱ) any one or more measures of the _{KH 2 PO 4, CaCl 2 ·} 2H 2 O, MgSO 4 · 7H 2 O, NaNO 3, K 2 HPO 4, NaCl, H 3 BO ₃ , and Na ₂ EDTA in a synthetic medium.

In one aspect of the present invention, the method for selecting microalgae having excellent lipid productivity may be such that the lipid content using the exhaust gas of the above ii) has a higher content than the lipid content using commercial carbon dioxide.

As used herein, the term " exhaust gas " may include all gaseous substances generated by burning, synthesizing or decomposing a specific substance. Specifically, the exhaust gas in the present specification may include gas discharged from a thermal power plant, and such exhaust gas may include carbon dioxide.

As used herein, the term " microalgae " includes all single-celled organisms that are photosynthetic with photosynthetic pigments, and include, without limitation, algae that survive in freshwater and / or seawater. Specifically, the microalgae of the present specification can be species that survive well in an acidic or salty environment. For example, the microalgae of the present specification may be species exhibiting excellent viability in an environment of pH 4 to 6.

In the present specification, "microalgae of exhaust gas resistance" may include normal microalgae in the above-mentioned exhaust gas even when growth of microalgae as in Example 1 is concerned.

In one aspect of the present invention, it is preferable that the microalgae are cultured at 15 to 35 占 폚, pH 5 to 8. Also, BBM culture medium _{(KH 2 PO 4, CaCl 2} · 2H 2 O, MgSO 4 · 7H 2 O, NaNO 3, K 2 HPO 4, NaCl, H 3 BO 3, Na 2 medium containing EDTA and trace elements) It is more preferable to cultivate the cells. The trace elements are _{ZnSO 4 · 7H 2 O, MnCl} 2 · 4H 2 O, MoO 3, CuSO 4 · 5H 2 O and Co (NO _{3) 2 ·} may be at least one selected from the group consisting of 6H ₂ O.

In one aspect of the present invention, the lipid of the algae can be analyzed by Bligh and Dyer (CHCl ₃ + MeOH) method or the like. Fatty acid was analyzed by GC-FID (BRUKER) by fatty acid methyl ester by methylation using sulfuric acid and methanol, and palmitic acid It was confirmed that 34 wt%, oleic acid and 13 wt% of gamma-linolenic acid were contained in the total fatty acid content, 13 wt% and 35 wt%, respectively, The present invention can also include a method for producing biodiesel using the lipid.

Hereinafter, the present invention will be described in more detail by way of examples. It should be noted, however, that the following examples are illustrative of the invention and are not intended to limit the scope of the invention.

[Example 1] Selection of microalgae species

Experiments were conducted to select microalgae that can normally grow in a high concentration thermal power plant. A selection experiment was carried out in the culture medium in which the exhaust gas carbon dioxide concentration 14.1 volume% was continuously injected from the basic medium Bold Basal Media (hereinafter referred to as "BBM"). Three kinds of microalgae shown in Table 1 below were inoculated into the culture medium and incubated for 5 days on a constant-temperature shaker. At this time, the culture conditions were maintained at a temperature of 27 ° C, pH 6.0-6.5, stirring of 150 rpm, and illumination of 100 μmol / m 2 -sec. After the incubation, the degree of growth of each microalgae was obtained by using OD (optical density at 680 nm) Hach DR-2800 absorption spectrophotometer.

Algal species Strain ID Accession Number OD Scenedesmus obliquus KGE 9 HE861884 0.50 Nephroselmis sp . KGE 11 HE861883 0.10 Micractinium reisserier KGE 22 HE974910 0.15

Also, Acutodesmus The OD value of the obliquus (KGE30) was measured to be 1.15 as a result of measurement of the absorbance by the same method as that for measuring the degree of growth of the microalgae. Acutodesmus, the strain with the highest growth rate obliquus (KGE30) was deposited at Korea Biotechnology Research Institute on April 21, 2015. Since the OD value of the KGE 30 was high, it was judged to be easy to test the exhaust gas at a high concentration and it was used in the following experiments.

[Example 2] Analysis of exhaust gas components and replacement of culture liquid

After collecting the exhaust gas from the Yeongdong Thermal Power Plant, the components in the exhaust gas were analyzed using a testo 350K combustion efficiency analyzer (Testo, Germany). As a result, NOx 200 ppm, SOx 50 ppm, CO 100 ppm, and CO ₂ 14.1%. The exhaust gas was transferred by a pump and then replaced at a rate of 0.5 L / min for 30 minutes in the culture solution contained in the experimental bottle through a distributor.

[Example 3] Microalgae culture

The flask was filled with the test bottle so that the volume of the BBM became 300 mL, and the exhaust gas was pergaged (experimental group). The initial concentration of microalgae (KGE30) was adjusted to an initial OD ₆₈₀ concentration of 0.015 in the experimental bottle. The experimental vessel was incubated for 5 days at a temperature of 27 ° C, pH 5.5, 150 rpm, and 120 μmol / ㎡-sec using a thermostatic shaker equipped with a fluorescent lamp. At this time, in order to confirm the effect of the exhaust gas, 14.1% of commercial CO ₂ (Japanese gas) and each of the bottles in which atmospheric air was replaced were used as a control group.

The experimental results are shown in Fig. Experimental results showed that the growth rate and maximum growth rate were better in the case of using the exhaust gas than in the control group. In addition, the growth rate and maximum growth rate were similar in the control group and the experimental group cultured with commercial carbon dioxide. Therefore, there was no particular problem in the growth of microalgae when cultivating the microalgae using the exhaust gas as compared with the case of using the conventional commercial carbon dioxide.

[Example 4] Analysis of lipid content

The microalgae, Acutodesmus ovicus , which had been cultivated in the culture medium of Example 3 for 4 days in the exhaust gas (experimental group) and the commercial carbon dioxide (control group) Obliquus KGE 30 was recovered and centrifuged at 3500 rpm in a swing type centrifuge (Hanil, Korea). Centrifuged microalgae Akudodesumesusuburikusu ( Acutodesmus Obliquus ) KGE 30 was lyophilized for 3 days using a freeze drier, 500 mg of which was collected and placed in a container for lipid extraction (a centrifugeable glass container and a lid with a Teflon liner inserted) ml and 2.5 ml of methanol were added using a glass pipette. Thereafter, the sample to which the solvent was added to the microalgae KGE30 was thoroughly mixed with a high-speed stirrer (Scinetific Ind) for 5 minutes and crushed for 30 minutes using a sonicator (Bransonic) to start extraction. The shredded microalgae KGE30 was extracted with stirring (30 ° C, 150 rpm) for one day on a constant-temperature shaker at 150 rpm and 30 ° C. 1.25 ml of chloroprene was added to the extracted sample using a glass pipette and further stirred for 2 hours on a thermostatic shaker. To the extracted sample, 1.25 ml of distilled water was added using a micropipette, centrifuged, and the organic layer and the water layer were separated. The separated organic layer (primary extract) was transferred to a new container (with a teflon liner inserted into a centrifugeable glass container and lid) using a pasture pipette, and 1.25 ml of distilled water was added to the remaining material, followed by one more centrifugation . The resulting organic layer (secondary extract) and water layer were separated, and the organic layer (secondary extract) was separated using a pasture pipette to obtain a lipid extract by combining the primary extract and the secondary extract. To the lipid extract thus obtained, 5 ml of NaCl (5%) was added and mixed. After centrifuging, only the organic layer was transferred, and the organic layer was evaporated using a rotary vacuum evaporator to separate the pure lipids. The weight of the separated lipid was measured and the weight of lipid was calculated to be 125 mg. The weight of the microalgae used was 500 mg. The total lipid content of microalgae KGE30 was calculated by substituting this into equation (1).

[Equation 1]

Lipid content (%) = dry weight of lipid extract (mg) / dry weight of microalgae (mg) X 100

The experimental conditions and experimental results are shown in Table 2 below.

Gas type Type of Wastewater or medium Cultivation period
(Day) Gas purging time
(min) Lipid content
( % biomass ) Commercial CO2
(Control group) BBM 4 30 12.3 ± 0.72 Flue gas CO2
(Experimental group) BBM 4 30 17.5 ± 2.25

As a result of the above lipid analysis, it was confirmed that the growth rate and growth amount of the lipid were increased by about 5% when the exhaust gas carbon dioxide was used even though the control group and the experimental group were similar. This can be a microalgae culture method for securing microalgae with high productivity in the production of lipids in the future.

[Example 5] Extraction and analysis of fatty acids

Fatty acid content and composition were determined by Lepage and Roy [Lepage, G., C.C. Roy (1984) Improved recovery of fatty acid through direct transesterification without prior extraction or purification, Journal of Lipid Research, 25, 1391-1396.

FAME Quantitative Standard Mix 37 comps. [AccuStandard, USA], a mixture of fatty acid methyl esters, was used as a standard. 2 mL of chloroform-methanol (2: 1, vol / vol) was added to the microalgae lipid sample of Example 4, which had been mass-measured in a glass tube [11 mL, DH.GL28020, Daihan Scientific, After injection, the mixture was mixed at room temperature for 10 minutes with a vortex mixer (Vorex Genius 3. Ika, Italy).

1 mL (500 μg / L) of chloroform containing an internal standard substance nonadecanoic acid (Sigma Co., USA), 1 mL of methanol, and 300 μL of sulfuric acid were sequentially added to a glass tube, Lt; / RTI > The tube was placed in a constant-temperature water bath and reacted at 100 ° C for 10 minutes. After cooling the tube to room temperature, 1 mL of distilled water was poured, mixed vigorously for 5 minutes with a mixer, and centrifuged at 4,000 rpm for 10 minutes to separate layers. The bottom layer (organic phase) was extracted with a disposable PP material syringe (Norm-ject, Germany) and analyzed by gas chromatography [Bruker] using a disposable 0.22 μm PVDF syringe filter [(Millex-Gv, Millipore, USA) Respectively. The results of the analysis are shown in Table 3 below.

Fatty acid composition Fatty acid composition ( % , w / w) Exhaust fumes Synthesis gas Palmitic acid (C16: 0) 34.0 43.4 Oleic acid (C18: 1n9c) 13.0 12.4 Linolelaidic acid (C18: 2n6t) 7.8 3.7 γ-Linolenic acid (C18: 3n6) 36.0 17.2 Other 9.2 23.3 Total 32.2 ± 0.33 (mg / g) 9.4 ± 0.37 (mg / g)

As a result of the experiment, the growth rate and the amount of growth were similar in the control group and the experimental group, but the total amount of fatty acid was in the control group (32.2 ± 0.33 (mg / g)) using the exhaust gas carbon dioxide (9.4 ± 0.37 mg / g)). This can be a microalgae culture method for securing microalgae with high productivity in fatty acid production later.

In addition, the fatty acid content of the experimental group was 3.22% higher than the total dry biomass of 1 g, and the fatty acid content was higher than that of the control group, which had a fatty acid content of about 0.94% based on 1 g of total dry biomass.

In addition, the content of unsaturated fatty acids (C18: 1 n9c), linolelaidic acid (C18: 2n6t) and γ-linolenic acid (C18: 3n6) increased from 33% to 56% Can be used as a raw material for production of high quality biodiesel.

The lipid content, fatty acid content, and unsaturated fatty acid content of the experimental group were higher than those of the control group as described above. It is considered that the lipid content in the microalgae was increased due to SOx and NOx which are known as toxic substances. In addition, the microalgae produced can be used as raw materials for functional foods such as omega 3, carotenoids and the like.

[Example 6] Maximal growth of microalgae was secured by injecting carbon dioxide into exhaust gas

The flask was filled with a test bottle so that the volume of the BBM became 300 mL, and the exhaust gas of Example 1 was purged. The initial concentration of microalgae KGE30 was adjusted to an initial OD ₆₈₀ concentration of 0.015 in the experimental bottle. Experimental containers were incubated for 16 days under conditions of temperature of 27 ° C, pH 5.5, 150 rpm, and 120 μmol / ㎡-sec, using a thermostatic shaker equipped with a fluorescent lamp. During the incubation, the cells were replanted on days 4, 8, and 11 for 30 min. The microalgae of the present invention emit OH ^- during growth and the pH is raised. When the pH is above 10, the growth is slowed down. After the substitution, the pH was decreased to about 7 after the substitution and the pH was restored to 11 as the growth progressed. The experimental results are shown in Fig.

Experimental results show that the maximum growth of the microalgae KGE 30 grown by the first displacement is increased to OD 1.15, OD 1.6 after the second substitution, and OD 2.9 after the fourth substitution of OD 2.5 after the third substitution. This is a method capable of culturing microalgae at a high concentration as a result of continuously increasing the maximum growth amount of microalgae using the exhaust gas carbon dioxide.

[Example 7] Removal of carbon source in exhaust gas

The test bottle was filled with 300 mL of BBM and purged of exhaust gas (experimental group) and commercial carbon dioxide (control group). The initial concentration of microalgae was adjusted to an initial OD ₆₈₀ value of 0.015 in the experimental bottle. The experimental vessel was incubated for 5 days at a temperature of 27 ° C, pH 5.5, 150 rpm, and 120 μmol / ㎡-sec using a thermostatic shaker equipped with a fluorescent lamp. At this time, TOC analyzer (Simadzu) was used to confirm the type and amount of carbon source present in the culture solution. The form of the carbon source was divided into inorganic carbon (TIC) and organic carbon (TOC), and the total amount of inorganic carbon and organic carbon was defined as total carbon (TC). For the analysis sample, the culture solution was sampled using a sterilized syringe in a culture vessel, the microalgae and the solution were separated using a PVDF filter, and the solution was injected into the TOC analyzer. The experimental results are shown in Fig.

Abbreviation:

TC (Total Carbnon)

TIC (Total Inorganic Carbon)

TOC (Total Organic Carbon)

As a result, the concentration of TIC decreased to near zero at 2 ~ 3 days when the culture reached its maximum. Exhaust gas Carbon dioxide was supplied with TIC in the culture medium with inorganic carbon, and microalgae were grown using all of them. This is a culture method that can be utilized to remove carbon dioxide in the exhaust gas through the continuous supply of carbon dioxide present in the exhaust gas.

The foregoing description is merely illustrative of the technical idea of the present invention and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the drawings disclosed in the present invention are intended to illustrate and not limit the technical spirit of the present invention, and the scope of the technical idea of the present invention is not limited by these drawings. The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

Korea Biotechnology Research Institute KCTC18367P 20150406

Claims (20)

Acutodesmus obliquus ) KGE 30 microalgae. The method according to claim 1,
Wherein said microalgae is KCTC18367P. A method for simultaneously cultivating a microalgae and removing carbon dioxide in an exhaust gas, the method comprising culturing a microalgae by supplying an exhaust gas containing carbon dioxide as a carbon source to the microalgae. A method for promoting the growth of microalgae, comprising feeding microalgae with an exhaust gas containing carbon dioxide as a carbon source to cultivate a microalgae. A method for increasing lipid production in a microalgae, comprising feeding microalgae with an exhaust gas containing carbon dioxide as a carbon source to cultivate microalgae. A method for increasing production of fatty acids in microalgae, comprising feeding microalgae with an exhaust gas containing carbon dioxide as a carbon source to cultivate microalgae. 7. The method according to any one of claims 3 to 6,
Wherein the carbon dioxide concentration in the exhaust gas is from 1 vol% to 20 vol% concentration based on the total exhaust gas volume. 7. The method according to any one of claims 3 to 6,
Wherein the exhaust gas is an exhaust gas of a thermal power plant. 7. The method according to any one of claims 3 to 6,
Wherein the exhaust gas is injected at each of the growth slowing points of the microalgae. 7. The method according to any one of claims 3 to 6,
Wherein the microalgae are microalgae which exhibit growth of 50% or more of the growth rate during cultivation at an optimal pH (optimal pH) of the microalgae when cultured in an environment of pH 5.5. 11. The method of claim 10,
The microalgae may be selected from the group consisting of Scenedesmus sp . ), Nephroselmis sp . , Micractinium sp . sp . ) And Acutodesmus sp. ). 11. The method of claim 10,
The microalgae may be selected from the group consisting of Scenedesmus obliquus , Micractinium reisseri , and Acutodesmus obliquus . 7. The method according to any one of claims 3 to 6,
The microalgae may be selected from the group consisting of Acutodesmus obliquus ) KGE 30 method. 14. The method of claim 13,
Wherein said microalgae is KCTC18367P. 7. The method according to any one of claims 3 to 6,
Culturing the microalgae comprises culturing the microalgae by supplying wastewater in addition to the exhaust gas. 7. The method according to any one of claims 3 to 6,
Wherein the wastewater is wastewater containing any one of livestock manure, mine drainage, domestic wastewater and food wastewater. The method according to claim 6,
Wherein the fatty acid content is 1.00% (w / w) or more of the total lipid. As a method for selecting microalgae having excellent lipid productivity,
The method comprising measuring one or more of the following:
I) measuring the amount of growth of microalgae by culturing microalgae using exhaust gas and commercial carbon dioxide, respectively;
Ii) Measurement of individual lipid content by culturing microalgae using exhaust gas and commercial carbon dioxide, respectively. 19. The method of claim 18,
The ⅰ) or ⅱ) any one or more measures of the _{KH 2 PO 4, CaCl 2 ·} 2H 2 O, MgSO 4 · 7H 2 O, NaNO 3, K 2 HPO 4, NaCl, H 3 BO 3, and Na ₂ EDTA And a method for screening microalgae having excellent lipid productivity. 19. The method of claim 18,
The microalgae having a lipid content higher than that of commercial carbon dioxide by the exhaust gas of the step (ii) is selected as microalgae having excellent lipid productivity.

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