KR20170003001A - Agent for dissolving plaque matrix - Google Patents

Abstract

Disclosed is an agent for dissolving a plaque matrix. An oral composition comprises a solvent including polyethylene glycol for dissolving a plaque matrix inside the mouth, and an antibacterial agent for removing germs. The oral composition densifies plaque by forming a sticky glomeration, and dissolves a plaque matrix which helps reattachment of germs to prevent germs from detaching from the tooth surface. Also, antibacterial components penetrate inside the plaque. Thus, the agent effectively removes plaque.

Description

{AGENT FOR DISSOLVING PLAQUE MATRIX}

Pragm substrate solubilizers are disclosed herein.

The plaque is an organic tooth attachment consisting of microorganisms and their products attached to the surface of the tooth. It can not be removed by the internal action of the oral cavity and is removed only by physical methods such as brushing. Therefore, it is difficult to remove plaque that does not reach toothbrushing, which causes dental caries and gum disease.

Oral antimicrobial agents generally used have excellent antibacterial activity against microorganisms themselves, but antibacterial components are difficult to penetrate into plaque in microorganisms and substrates such as plaque. In particular, the poorly soluble glucan component, which is a polysaccharide derived from bacteria, which is a major component of the plaque matrix, forms a sticky compact so that the bacterial membrane is dense and the bacteria do not fall off from the tooth surface. The antimicrobial agent has a problem that it is difficult to effectively remove such plaque substrate.

Korean Patent Publication No. 10-2010-0003411

In one aspect, the present invention aims to provide a pragmatic substrate dissolving agent and oral composition capable of effectively removing praes by dissolving a prage substrate that makes the oral antimicrobial agent penetrate into the pore, .

In one aspect, the techniques disclosed herein provide a plaque matrix solubilizer comprising polyethylene glycol as an active ingredient.

In an exemplary embodiment, the plaque substrate may be one comprising oral bacterial glucan.

In an exemplary embodiment, the polyethylene glycol is selected from the group consisting of PEG-4, PEG-6, PEG-8, PEG-12, PEG-32, PEG-60, PEG- ≪ / RTI >

In another aspect, the techniques disclosed herein are directed to the use of the Prag substrate solubilizer; And an antimicrobial agent.

In an exemplary embodiment, the polyethylene glycol may be included in an amount of 0.1 to 10% by weight based on the total weight of the composition.

In an exemplary embodiment, the antimicrobial agent may be included in an amount of 0.001 to 3% by weight based on the total weight of the composition.

In one exemplary embodiment, the antimicrobial agent is selected from the group consisting of cetylpyridinium chloride, chlorhexidine digluconate, chlorhexidine hydrochloride, benzenethonium chloride, benzalkonium chloride, zinc chloride, zinc citrate, zinc lactate, isopropylmethyl phenol, , Eucalyptol, levomentol, and the like.

In one aspect, the plague substrate solubilizer disclosed herein forms a sticky compact to tighten the bacterial membrane and prevent the bacteria from falling off the surface of the dentifrice. Bacteria-derived polysaccharide insoluble glucan It has an effect of dissolving the components.

The oral composition disclosed herein includes a solvent for dissolving the oral plaque substrate containing polyethylene glycol and an antibacterial agent for removing bacteria so that the antibacterial component penetrates into the inside of the prage to effectively remove the prage, There is an effect that can remove the plaque until.

FIG. 1 is a graph showing the OD value of the mouse wash eradication efficacy according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

In one aspect, the techniques disclosed herein provide a plaque matrix solubilizer comprising polyethylene glycol as an active ingredient.

As used herein, the term "prag" refers to a colorless bacterial film continuously formed on a tooth surface, and is also called a plaque or biofilm.

For example, when a germ such as Streptococcus mutans secretes a sticky polysaccharide called glucan out of the cell using sugar, the polysaccharide causes the cells to clump together. This is because Streptococcus mutans produces an enzyme called glucosyltransferase, which synthesizes a polysaccharide called glucose, with sugar as a substrate.

Glucan is not soluble in water and has a very high tackiness, so it attaches to the surface of teeth and stagnates the plaque. Therefore, it is very important to dissolve the poorly soluble glucan in order to remove the prage.

As a result, plaque is a sticky substance made by bacteria living in the mouth, such as Streptococcus mutans, and is a sticky film adhered to the surface of teeth and gums. The membrane is composed of a dental floss membrane in which oral bacteria are trapped in a network of dextran, a glucose polymer. These plaques are generally known to be a major cause of tooth decay, gingival diseases and gum disease, which are important diseases in teeth and surrounding tissues.

In addition, when the dental floss is left for a long time without removing the dental floss, it forms an insoluble salt by combining with calcium, magnesium and phosphorus in the oral saliva to form tartar having hardness similar to that of a tooth. As the teeth continue to form, the gingiva and dental gut are destroyed, causing bleeding easily, and inflammation caused by bacteria in the mouth, resulting in gingivitis and periodontal disease.

As used herein, a "plaque matrix " refers to a sticky material that acts to attach and fix plaque to a tooth surface or gums. Plags consist of germs and sticky substances derived from these bacteria or saliva, which are present in the network of adhesive substances derived from bacteria or saliva. That is, the Prag substrate refers to a sticky substance containing glucan or the like derived from bacteria or saliva.

In an exemplary embodiment, the plaque substrate may be one comprising oral bacterial glucan.

In one exemplary embodiment, the oral bacteria are selected from the group consisting of Streptococcus mutans , Streptococcus sanguinis , Streptococcus sobrinus , Streptococcus ratti , Streptococcus mutans , For example, Streptococcus criceti , Streptococcus anginosus , Streptococcus gordonii , Actinobacillus actinomycetemcomitans , Fusobacterium nucleatum ( Fusobacterium nucleatum) ), Prevotella intermedia , and Porphyromonas gingivalis . ≪ Desc / Clms Page number 2 > In another aspect, an oral composition comprising a plague substrate solubilizer according to the present disclosure has an antibacterial activity against said oral bacteria.

In an exemplary embodiment, the polyethylene glycol is selected from the group consisting of PEG-4, PEG-6, PEG-8, PEG-12, PEG-32, PEG-60, PEG- ≪ / RTI >

In another aspect, the techniques disclosed herein are directed to the use of the Prag substrate solubilizer; And an antimicrobial agent.

In an exemplary embodiment, the polyethylene glycol is included in an amount of from 0.1 to 10% by weight based on the total weight of the composition, thereby minimizing irritation to the oral mucosa, thereby increasing use stability and exhibiting remarkably improved plaque removal efficacy It is effective. In this respect, the polyethylene glycol may be present in an amount of at least 0.2%, at least 0.3%, at least 0.4%, at least 0.5%, at least 0.6%, at least 0.7%, at least 0.8% At least 9 wt.%, At most 8 wt.%, At most 7 wt.%, At most 6 wt.%, At most 5 wt.%, At most 4 wt.%, 3 wt% or less, 2.5 wt% or less, or 2 wt% or less.

In an exemplary embodiment, the antimicrobial agent is included in an amount of 0.001 to 3% by weight based on the total weight of the composition, thereby minimizing irritation to the oral mucosa, thereby increasing the stability of use and exhibiting a remarkably improved effect of removing plaque . In this regard, the antimicrobial agent may be present in an amount of 0.002 wt.%, 0.003 wt.%, 0.004 wt.%, 0.005 wt.%, 0.006 wt.%, 0.007 wt.%, 0.007 wt.%, 0.008 wt. Not more than 0.01% by weight, not less than 0.05% by weight or not less than 0.1% by weight, not more than 2.5% by weight, not more than 2% by weight, not more than 1.5% by weight, not more than 1% by weight, not more than 0.9% Up to 0.6 wt.%, Up to 0.5 wt.%, Up to 0.4 wt.%, Up to 0.3 wt.%, Up to 0.2 wt.%, Up to 0.1 wt.

In one exemplary embodiment, the antimicrobial agent is selected from the group consisting of cetylpyridinium chloride, chlorhexidine digluconate, chlorhexidine hydrochloride, benzenethonium chloride, benzalkonium chloride, zinc chloride, zinc citrate, zinc lactate, isopropylmethyl phenol, , Eucalyptol, levomentol, and the like.

In one exemplary embodiment, the oral composition may be a formulation of a toothpaste, a mouth wash, a cream or a patch.

The dentifrice in the present specification includes liquid or solid dentifrice, and there is no limitation in its formulation, and mouthwash is also referred to as an oral dentifrice. Further, the cream includes an oral ointment, and the patch means to be used by being stuck on the tooth surface.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Examples 1-2. Mouse wash manufacture

The mouthwash oral compositions of Examples 1 and 2 were prepared according to the following composition.

PEG, cetylpyridinium chloride, sodium benzoate, and sodium saccharin were dissolved in purified water until they became transparent, and a solution prepared by mixing the flavor and polyoxyethylene hardened castor oil was added to a solution in which the water soluble ingredients were dissolved, A composition was prepared.

< Example  1>

PEG-75 1%

Cetylpyridinium chloride 0.05%

Sodium benzoate 0.3%

Flavor 0.1%

Saccharin sodium 0.05%

Polyoxyethylene hardened castor oil 0.5%

Purified water quantity

&Lt; Example 2 >

PEG-8 1%

Cetylpyridinium chloride 0.05%

Sodium benzoate 0.3%

Flavor 0.1%

Saccharin sodium 0.05%

Polyoxyethylene hardened castor oil 0.5%

Purified water quantity

Comparative Examples 1 and 2. Mouse wash manufacture

The mouth wash oral compositions of Comparative Examples 1 and 2 were prepared according to the following composition, while the mouth wash was prepared in the same manner as in the above Examples.

&Lt; Comparative Example 1 &

Cetylpyridinium chloride 0.05%

Sodium benzoate 0.3%

Flavor 0.1%

Saccharin sodium 0.05%

Polyoxyethylene hardened castor oil 0.5%

Purified water quantity

&Lt; Comparative Example 2 &

PEG-800 1%

Cetylpyridinium chloride 0.05%

Sodium benzoate 0.3%

Flavor 0.1%

Saccharin sodium 0.05%

Polyoxyethylene hardened castor oil 0.5%

Purified water quantity

Experimental Example 1. Confirmation of elimination of plaque

The plaque elimination ability of the mouse wash prepared in the above Examples and Comparative Examples was confirmed by the following method.

1) Streptococcus mutans (KCTC 3065) was cultured in Brain Heart infusion medium for 24 hours at 35 ° C.

2) The hydroxyapatite plate, which is a dental primer, was immersed in 70% ethanol for 30 minutes, sterilized, and washed three times with sterile ionized water. The washed hydroxyapatite plates were rubbed one by one into a 24-well plate.

3) 1 ml of sterile ionized water containing 1% mucin was added to each well, and the mixture was stored at 35 ° C for 30 minutes to coat the hydroxyapatite plate with mucin.

4) The cultured Streptococcus mutans culture was inoculated and cultured at 35 DEG C for 72 hours to form a biofilm.

5) Each mouse wash of the Examples and Comparative Examples was diluted 20% in PBS buffer, and 1 ml was taken out. The treated hydroxyapatite plate was treated for 3 minutes, and washed three times with sterile ionized water.

6) 0.1% crystal violet solution was treated, stained and completely dried, and 70% ethanol was added to extract the dye.

7) The concentration of the extracted dye diluent was measured by optical density OD (Optical Density, 590 nm).

As a result, as shown in FIG. 1, the OD value of mouse wash according to the example was significantly lower than that of the mouse wash according to the comparative example, indicating that the biofilm was removed. Accordingly, it has been confirmed that the oral composition containing polyethylene glycol disclosed in the present invention has an effect of effectively dissolving the bio-film by dissolving the protein substrate.

Having described specific portions of the present invention in detail, it will be apparent to those skilled in the art that this specific description is only a preferred embodiment and that the scope of the present invention is not limited thereby. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (7)

A plaque matrix solubilizer comprising polyethylene glycol as an active ingredient.
The method according to claim 1,
Wherein the Prag substrate comprises oral bacterial glucan.
The method according to claim 1,
Wherein the polyethylene glycol is selected from the group consisting of PEG-4, PEG-6, PEG-8, PEG-12, PEG-32, PEG-60, PEG- .............
13. The Prag substrate solubilizer according to any one of claims 1 to 3; And
An oral composition for removing plaque comprising an antibacterial agent.
5. The method of claim 4,
Wherein the polyethylene glycol is contained in an amount of 0.1 to 10% by weight based on the total weight of the composition.
5. The method of claim 4,
Wherein the antibacterial agent is contained in an amount of 0.001 to 3% by weight based on the total weight of the composition.
5. The method of claim 4,
Wherein the antimicrobial agent is selected from the group consisting of cetylpyridinium chloride, chlorhexidine digluconate, chlorhexidine hydrochloride, benzenethonium chloride, benzalkonium chloride, zinc chloride, zinc citrate, zinc lactate, isopropylmethyl phenol, methyl salicylate, thymol, eucalyptol, levomenthol Wherein the oral composition is at least one selected from the group consisting of:

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