KR20170002162A

KR20170002162A - Kit for separating blood and method for separating blood

Info

Publication number: KR20170002162A
Application number: KR1020150092240A
Authority: KR
Inventors: 노정자
Original assignee: 노정자
Priority date: 2015-06-29
Filing date: 2015-06-29
Publication date: 2017-01-06
Also published as: KR101726948B1

Abstract

The present invention relates to a blood separation kit and a blood separation method for separating a desired component by centrifuging blood. The blood separation kit comprising: a body container in the form of a soft tube having an accommodation space therein and having an upper portion with an inlet, a lower portion below the upper portion, and a neck connecting the upper portion and the lower portion with a reduced cross section; An upper lid capable of closing said inlet; A clamp able to press the neck; .
According to such a configuration, the main body container is in the form of a soft tube having a neck, and the neck can be pressed and blocked by the forceps, so that the structure is simple and can be manufactured at low cost and the desired component can be separated from the blood by a simple method A blood separation kit and a blood separation method can be provided.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood separation kit,

The present invention relates to a blood separation kit and a blood separation method for separating a desired component by centrifuging blood.

In general, blood cells in the blood are mixed with different components of specific gravity. When they are separated by centrifugation, they form layers.

BACKGROUND ART It is known that platelets in a small amount in blood have an excellent effect on regeneration of tissues. Therefore, it is known that treatment of tissue regeneration by administering Platelet Rich Plasma (PRP) to worn tissues due to degenerative changes Many methods have been tried, and PRP has been applied in various fields such as skin care and skin treatment.

The reason for platelet regeneration is that platelet cells contain abundant growth factors. Injecting high concentrations of platelets into the treatment site causes platelet growth factors to activate the stem cells and regenerate the damaged tissue.

In general, PRP extraction is performed by first centrifuging blood from the human body. The blood is centrifuged and separated into individual layers according to specific gravity. The composition of the blood consists of a solution component and a blood cell component (granule cell). There are red blood cells, white blood cells, platelets and very small amount of antibodies. These blood cells are mixed in a solution having a salinity of 0.9%. The majority of the double blood cells constitute red blood cells.

When the blood is centrifuged, the blood is separated into three layers. The red blood cells with the highest specific gravity are collected at the bottom of the container, platelets and white blood cells are gathered right up to form a thin layer, and there are almost no remaining grains Solution components are located. Thin layer is called PRP while platelets and white blood cells are intensively gathered. Plasma layer consisting of solution without blood granules above it is called PPP (Platelet Poor Plasma, hereinafter referred to as PPP).

In order to extract the PRP on the red blood cell layer, the syringe is inserted into the test tube, and the end portion of the syringe is drawn into the PRP layer to be sucked out. In this process, red blood cells are injected with PRP, and these red blood cells are injected into the tissue together with PRP when they are administered to the patient.

When red blood cells are injected into the patient's tissues together with PRP, the concentration of PRP is lowered and the therapeutic effect is lowered, which causes patients to exert severe pain on the operation site.

For this reason, several methods have been used to prevent the inclusion of red blood cells in PRP extraction. The most commonly used method is to reduce the diameter of the centrifuge tube in the area where the PRP layer is expected to be formed, and to reduce the contact area with the erythrocyte layer in proportion to the reduced cross-sectional area.

When this method is used, the thickness of the lower erythrocyte layer varies from person to person, and in many cases, PRP is not actually located after centrifugation at a region where PRP is expected to be formed.

Thus, in order to adjust the height of the red blood cell layer in the lower part of the separator, the height of the red blood cell layer is adjusted by turning a screw, which is usually in the form of a screw, at the bottom of the centrifuge tube. However, in such a centrifuge tube, when the centrifuge tube is rotated at a high speed of 3000 to 4000 rpm, a great escape pressure of blood is applied to the lower portion of the centrifuge tube, and blood may flow out through the screw portion.

As a result, additional components and precise fabrication are required to prevent leakage, and the cost of the centrifuge tube is inevitably increased, resulting in a higher final treatment amount.

Therefore, there is a need for a blood separation method which can separate PRP from centrifuged blood, can be manufactured at low cost with simple structure, and is easy to separate.

Korean Patent No. 10-1267379

SUMMARY OF THE INVENTION Accordingly, it is an object of the present invention to provide a blood separation kit and a blood separation method capable of separating PRP by a simple method by centrifuging blood, There is a purpose.

According to an aspect of the present invention, there is provided a blood separation kit including: an upper portion having an accommodation space therein and having an inlet; a lower portion below the upper portion; A flexible tubular body container having a neck; An upper lid capable of closing said inlet; A clamp able to press the neck; .

A thin plate-like first connection portion located at one side of the neck portion and connecting the upper portion and the lower portion adjacent to the neck portion; A second connecting portion located at the other side of the neck portion and connecting the upper portion and the lower portion adjacent to the neck portion; .

Further, the upper lid is hermetically connected to the inlet, and the upper lid can be separated from the inlet by applying a predetermined force.

Further, the forceps have a pressing portion for pressing the neck portion, and the pressing portion has inwardly protruding protruding portions facing each other.

According to another aspect of the present invention, there is provided a blood separation method for separating a desired component by centrifuging blood, the method comprising: an upper portion having a receiving space therein, an upper portion having an inlet, Providing a blood separation kit having a body container in the form of a flexible tube having a neck connecting a lower portion thereof, an upper lid capable of closing the inlet, and a clip capable of pressing the neck portion; Opening the upper lid and injecting hemorrhaged blood into the main container; Closing the inlet with the top lid; Centrifuging the main container in a centrifuge to separate blood into a red blood cell layer, a platelet rich plasma (PRP) layer and a platelet poor plasma (PPP) layer; Pressing the lower portion of the main container to place the PRP layer on the neck; Pressing the neck with the forceps to separate the PRP layer and the red blood cell layer; .

The blood separation kit may further include a thin plate-like first connection portion located at one side of the neck portion and connecting the upper portion and the lower portion adjacent to the neck portion, and a second connection portion located at the other side of the neck portion, And a second connection part in the form of a thin plate connecting the lower part.

According to the present invention, the main body container is in the form of a soft tube having a neck, and the neck can be pressed and blocked by a forceps, so that the structure is simple and can be manufactured at low cost and a desired component can be separated from the blood A blood separation kit and a blood separation method can be provided.

1 is a perspective view showing a blood separation kit according to an embodiment of the present invention.
Fig. 2 is a view showing a state in which the upper lid is separated from the main container in the blood separation kit of Fig. 1;
FIG. 3A is a cross-sectional view taken along the line A-A 'in FIG. 1, and FIG. 3B is a view showing a state in which the neck of the main body container is pressed by the forceps in FIG.
Figs. 4 to 9 are diagrams sequentially showing an embodiment of a method for separating blood using the blood separation kit of the present invention. Fig.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the drawings, like reference numerals refer to like elements throughout. The same reference numerals in the drawings denote like elements throughout the drawings.

1 is a perspective view showing a blood separation kit according to an embodiment of the present invention. Fig. 2 is a view showing a state in which the upper lid is separated from the main container in the blood separation kit of Fig. 1; FIG. 3A is a cross-sectional view taken along the line A-A 'in FIG. 1, and FIG. 3B is a view showing a state in which the neck of the main body container is pressed by the forceps in FIG.

When the blood is centrifuged, it is separated into a red blood cell layer, a PRP (Platelet Rich Plasma) layer and a PPP (Platelet Poor Plasma) layer. Three layers can be distinguished by color.

Since platelet cells contain abundant growth factors, therapeutic methods for regenerating tissue by administering a PRP layer to abraded tissues have been extensively attempted and have been applied to skin cosmetics and skin treatment.

The blood separation kit 100 according to the embodiment of the present invention can separate the PRP layer by a simple method after centrifugal separation of blood and can be manufactured at a low cost with a simple structure. The blood separation kit 100 includes a main body container 110, an upper lid 130, first and second connection portions 121 and 122, and a tongue 140.

The main body container 110 has a soft (or soft) tube shape and has a receiving space therein. The main body container 110 has a proper ductility so that the main body container 110 is pressed to allow the fluid inside to escape to the outside when a person presses the main body container 110 by hand. As long as the main container 110 has ductility, materials such as plastic and rubber can be appropriately selected by those skilled in the art. Further, it is preferable that the main body container 110 has appropriate elasticity so as to return to the original shape when the pressing force is released. The main container 110 may be provided with a scale 110a for indicating the amount of fluid.

The main body container 110 includes an upper portion 111 having an inlet 111a and a lower portion 113 below the upper portion 111. A neck portion 112 connecting the upper portion 111 and the lower portion 113 with a reduced cross- . The neck portion 112 and the upper portion 111 and the neck portion 112 and the lower portion 113 may be connected in a curved shape with a smooth round.

The upper lid 130 is formed so as to be able to close and close the inlet 111a of the main body container 110. [ The upper lid 130 may be made of soft plastic or rubber as in the case of the main body container 110. The upper lid 130 may be hermetically connected to the inlet 111a of the main body container 110. [ For example, the upper lid 130 may be joined to the inlet 111a of the main body container 110 with an inert gas such as nitrogen filled in the main body container 110. [

The upper lid 130 can be detached from the inlet 111a of the main body container 110 while the joined portion is detached by applying a predetermined force to the upper lid 130 as a human being holds and bit. The upper lid 130 can be fitted into the inlet 111a so as to close the inlet 111a again while being separated from the inlet 111a of the main container 110. [ When the upper lid 130 is fitted in the inlet 111a, airtightness or an appropriate sealing force can be provided.

The first connection part 121 is formed in a thin plate shape on one side of the neck part 112 and the second connection part 122 is formed on the other side of the neck part 112 in a thin plate shape. The first connection portion 121 and the second connection portion 122 connect the upper portion 111 and the lower portion 113 adjacent to the neck portion 112. The first connection portion 121 and the second connection portion 122 are formed to have a rigidity higher than that of the main body container 110.

Since the main body container 110 has a flexible tube shape and the middle neck portion 112 has a reduced sectional shape, deformation such as bending of the main container 110 during centrifugal separation tends to occur. When blood is introduced into the main container 110 and centrifugal separation is performed in the centrifugal separator, if the main container 110 is deformed such as being bent, centrifugal separation of blood can not be performed properly. The first connection part 121 and the second connection part 122 connect the upper part 111 and the lower part 113 of the main container 110 to reinforce the rigidity thereof, ) From being deformed.

The tongue 140 is formed to press the neck portion 112 of the main body container 110. The tongue 140 has an elongated pressing portion 141 for pressing the neck portion 112. The pressing portion 141 may have a protruding portion 141a protruding inward and facing each other.

Referring to FIG. 3A, in a cross-sectional view taken along the neck 112, first and second connection portions 121 and 122 are positioned on both sides of the neck portion 112.

3B, the neck portion 112 is pressed to be completely pressed by the protrusion 141a, so that fluid movement through the neck portion 112 in the main body container 110 can be blocked. If the protrusion 141a is not provided in the tongue 140, it may be difficult to press the neck 112 completely due to the thickness of the first connection portion 121 and the second connection portion 122.

The blood separation kit 100 as described above is in the form of a flexible tube having a single body, so that the blood separation kit 100 can be manufactured at low cost with a simple structure. Further, as described later, the PRP layer can be separated from the blood by a simple method without complicated process using the blood separation kit 100 of the present invention.

Hereinafter, an embodiment for separating components of blood using the blood separation kit of the present invention will be described with reference to FIGS. 4 to 9. FIG. Figs. 4 to 9 are diagrams sequentially showing an embodiment of a method for separating blood using the blood separation kit of the present invention. Fig.

Referring to FIG. 4, a blood separation kit 100 of the same type as shown in FIGS. 1 and 2 is provided. The blood separation kit 100 has a main body container 110 having a flexible tube shape and having a receiving space therein. The main body container 110 includes an upper portion 111 having an inlet 111a, a lower portion 113 below the upper portion 111 and a neck portion 112 connecting the upper portion 111 and the lower portion 113 while the cross- . The blood separation kit 100 further includes an upper lid 130 capable of closing the inlet 111a of the main container 110 and a second lid 130 connecting the first lid 121 and the second lid 122, And a gripper 140 which can be pressurized.

Next, referring to FIG. 5, the upper lid 130 is opened and the blood in the main container 110 is injected with blood. The blood can be injected using the injector 10, and the anticoagulant can also be injected into the main container 110 at this time.

6, the upper lid 130 closes the inlet 111a of the main container 110 again. At this time, the inlet 111a of the main container 110 may be closed by a member other than the upper lid 130.

Next, the main container 110 is centrifuged in a centrifuge to cause blood to be separated into the red blood cell layer 21, the PRP layer 22, and the PPP layer 23. At this time, the PRP layer 22 is positioned below the neck 112 of the main body container 110.

Next, referring to FIG. 7, the operator presses the lower portion of the main body container 110 with the force, so that the PRP layer 22 is positioned on the neck portion 112.

Next, referring to FIG. 8, the neck 112 is pressed against the tongue 140, and the PRP layer 22 and the red blood cell layer 21 are separated by the tongue 140.

9, when the PRP layer 22 and the red blood cell layer 21 are separated by the forceps 140, the upper lid 130 is opened and the syringe 10 is inserted into the main container 110. [ So that only the PRP layer 22 can be taken out. Alternatively, the PRP layer 22 and the PPPP layer 23 may be taken out together with the syringe 10, or the PRP layer 22 and the PPPP layer 23 may be pulled out together by turning the main body container 110 upside down. Since the upper lid 130 is made of soft plastic or the like and the syringe needle is inserted into the upper lid 130 without opening the upper lid 130, the PRP layer 22 and the like in the main container 110 are removed It is possible.

According to the blood separation method of the present invention as described above, the blood is centrifugally separated without complicated processes, and the PRP layer 22 is positioned on the neck 112 by pressing the lower part of the main body container 110, The PRP layer 22 and the red blood cell layer 21 can be separated from each other. Then, the desired component can be separated from the blood by withdrawing only the PRP layer 22 with the syringe 10 or pulling out the PRP layer 22 and the PPPP layer 23 together.

The length or width of the neck portion 112 in the main body container 110 may be appropriately set so that the PRP layer 22 and the red blood cell layer 21 can be separated well. The length, shape, and width of the tongue 140 can also be appropriately designed so that the PRP layer 22 and the red blood cell layer 21 are best separated from the main container 110.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention will be.

10: Syringe
21: Red blood cell layer
22: PRP layer
23: PPP layer
100: Blood separation kit
110: main container
111: upper
111a: entrance
112: neck
113: Lower
121: first connection part
122: second connection portion
130: upper lid
140: tongs
141:
141a:

Claims

A blood separation kit comprising:
A container body in the form of a flexible tube having a receiving space therein and having an inlet with an upper portion, a lower portion below the upper portion, and a neck connecting the upper portion and the lower portion with a reduced cross section;
An upper lid capable of closing said inlet;
A clamp able to press the neck;
And a blood separator.

The method according to claim 1,
A first connecting portion located at one side of the neck portion and connecting the upper portion and the lower portion adjacent to the neck portion;
A second connecting portion located at the other side of the neck portion and connecting the upper portion and the lower portion adjacent to the neck portion;
Further comprising:

3. The method according to claim 1 or 2,
The top lid is hermetically connected to the inlet,
Wherein the upper lid can be detached from the inlet by applying a predetermined force.

3. The method of claim 2,
Wherein the clamp has a pressing portion for pressing the neck portion, and the pressing portion protrudes inward and has protruding portions facing each other.

A blood separation method for separating a desired component by centrifuging blood,
A main container in the form of a flexible tube having a receiving space therein and having an inlet, an upper portion with an inlet, a lower portion below the upper portion, and a neck connecting the upper portion and the lower portion with a reduced cross section, And a blood separating kit having a clip capable of pressing the neck portion;
Opening the upper lid and injecting hemorrhaged blood into the main container;
Closing the inlet with the top lid;
Centrifuging the main container in a centrifuge to separate blood into a red blood cell layer, a platelet rich plasma (PRP) layer and a platelet poor plasma (PPP) layer;
Pressing the lower portion of the main container to place the PRP layer on the neck;
Pressing the neck with the forceps to separate the PRP layer and the red blood cell layer;
Gt;

6. The method of claim 5,
The blood separation kit includes a thin plate-like first connection portion located at one side of the neck portion and connecting the upper portion and the lower portion adjacent to the neck portion, and a second connection portion located at the other side of the neck portion, And a second connection part of a thin plate shape connecting the lower part.