KR20170001386A - Floating structure and reagent transformation system having the same - Google Patents

Floating structure and reagent transformation system having the same Download PDF

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Publication number
KR20170001386A
KR20170001386A KR1020150091306A KR20150091306A KR20170001386A KR 20170001386 A KR20170001386 A KR 20170001386A KR 1020150091306 A KR1020150091306 A KR 1020150091306A KR 20150091306 A KR20150091306 A KR 20150091306A KR 20170001386 A KR20170001386 A KR 20170001386A
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KR
South Korea
Prior art keywords
fluid
liquid
reacted
present
floating
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KR1020150091306A
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Korean (ko)
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KR101753961B1 (en
Inventor
김중배
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고려대학교 산학협력단
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Priority to KR1020150091306A priority Critical patent/KR101753961B1/en
Priority to PCT/KR2016/006864 priority patent/WO2016209063A2/en
Priority to US15/739,445 priority patent/US10888839B2/en
Publication of KR20170001386A publication Critical patent/KR20170001386A/en
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Publication of KR101753961B1 publication Critical patent/KR101753961B1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/28—Moving reactors, e.g. rotary drums
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/30—Loose or shaped packing elements, e.g. Raschig rings or Berl saddles, for pouring into the apparatus for mass or heat transfer
    • B01J19/305—Supporting elements therefor, e.g. grids, perforated plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J8/00—Chemical or physical processes in general, conducted in the presence of fluids and solid particles; Apparatus for such processes
    • B01J8/008—Details of the reactor or of the particulate material; Processes to increase or to retard the rate of reaction
    • B01J8/009—Membranes, e.g. feeding or removing reactants or products to or from the catalyst bed through a membrane
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor

Abstract

The present invention relates to a floating structure. The floating structure comprises: body parts which support materials being reacted; and at least one floating part which is connected to the body parts and makes the body parts float on surface of two liquids having different density while preventing the body parts from being mixed with each other. The materials being reacted are formed to react with reagents on one of the two liquids. So, the present invention serves as a reagent transformation system having the floating structure.

Description

[0001] Floating structure and reagent conversion system having same [0002]

The present invention relates to a floating structure and a reaction material conversion system having the same.

Conventionally, a technique of converting into a product by an arbitrary catalyst and a technique of selectively converting only a specific substance by an arbitrary biosensor have been proposed so that a conversion reaction can be performed based on mass transfer between two liquid phases separated without another structure It was designed.

However, in such a case, there may arise a problem that the efficiency of the reaction is lowered because the substances and the bioelements which perform the catalytic reaction are spread evenly in one liquid.

On the other hand, in the past, there has been no device capable of protecting the catalyst material and the bioelement for a long time from the change of the external environment in order to complete the reaction.

In addition, there has been no floating body capable of floating at two liquid phase interfaces, which is important for catalytic reactions and coupling reactions occurring at two liquid phase interfaces in the past, and it has been difficult for reactants or product materials to move between two liquid phases.

An embodiment of the present invention is to provide a suspended structure capable of supporting a material to be reacted and capable of protecting the material to be reacted for a long period of time from changes in the external environment and a reaction material switching system having the same.

In one embodiment of the present invention, a floating structure capable of increasing the efficiency of conversion and coupling reaction by facilitating contact between a reaction material and a reaction material in a floating state at an interface between two liquids which are not mixed, and a reaction To provide a material conversion system.

Also, an embodiment of the present invention is to provide a floating structure for selectively detecting only a specific substance using a specific binding specific to a substance, and a reaction material conversion system having the same.

According to an aspect of the present invention, there is provided a liquid container comprising: a body for supporting a material to be reacted; and at least one floating part coupled to the body part to allow the body part to float on an interface between two liquids having different densities, Wherein the reactant material is formed to react with a reactant present in any one of the two liquids.

Wherein the two liquids include a first liquid that does not interact with each other and a second liquid that is less dense than the first liquid and that is located on the first liquid, Is greater than the density of the second liquid and less than the density of the first liquid, and can float on the first liquid.

In this case, the body may include a first body and a second body, and the first body and the second body may be positioned to support the material to be reacted.

At this time, each of the first body and the second body may be provided with an opening through which the reaction material can approach from the outside to the inside.

At this time, each of the first body and the second body may be a latticed planar structure.

At this time, the first body and the second body may be arranged in parallel to each other in the vertical direction, and the second body may be disposed adjacent to the surface of the first liquid.

At this time, the first body and the second body may have the same shape.

At this time, the first body and the second body may be made of polyacrylonitrile, nylon, polyester, polyurethane, polyvinyl chloride, polystyrene, cellulose, chitosan, polylactic acid, polylactic-co- A carbon nanotube, a carbon compound, and a silica nanoparticle, or a magnetic nanoparticle composed of at least one of glycolic acid polycaprolactone, collagen, polypyrrole, polyaniline and poly (styrene-co-maleic anhydride) .

In this case, the apparatus may further include a fixing member positioned between the first body and the second body, and the material to be reacted may be fixed to the surface of the core member.

At this time, the fixing member may be a nano structure.

In this case, the floating portion may include a first floating body coupled to one end of the body portion and a second floating body coupled to the other end of the body portion, wherein the first floating body and the second floating body partially surround the body portion, And can be moved up and down while being immersed in the two liquids to float on the interface of the liquid.

At this time, the first subfluid or the second subfluid is formed with a fluid storage portion which is a space in which a fluid can be stored, and is connected to the first subfluid or the second subfluid, And a pump for introducing and exiting the refrigerant.

At this time, the fluid flowing into and out of the fluid reservoir through the pump may be air or the two liquids.

At this time, protrusions are formed at both ends of the body portion, and each of the first and second inflow fluids may have an engagement groove coupled to the protrusion.

At this time, the body portion and the floating portion may be formed of any one or more of polymer materials such as acrylonitrile-butadiene-styrene resin (ABS), polycarbonate (PC), polyvinyl alcohol (PVA), and polystyrene (PS).

The nano structure may be at least one selected from the group consisting of polyvinyl alcohol, polyacrylonitrile, nylon, polyester, polyurethane, polyvinyl chloride, polystyrene, cellulose, chitosan, polylactic acid, polylactic- A carbon nanotube, a carbon compound and a silica nanoparticle, and a magnetic nanoparticle composed of at least one of polyacrylonitrile, polycaprolactone, collagen, polypyrrole, polyaniline and poly (styrene-co-maleic anhydride) have.

At this time, the nanostructure includes a functional group, and the functional group reacts with the substance to be reacted, so that the substance to be reacted can be bound to the nanostructure.

At this time, the substance to be reacted may be bound to the nanostructure by being adsorbed on the nanostructure.

At this time, the material to be reacted may include a catalyst material or a biomolecule.

At this time, the catalytic material may include an enzyme including at least one of carbonic anhydrase, glucose oxidase, lipase, acylase, lactonase, protease, peroxidase, and Acinetobacter calcoaceticus. , Alcaligenes odorans, Aromatoleum aromaticum, Geobacter metallireducens, Dechloromonas aromatic, Arthrobacter sp., And the like. Microcrystalline nanoparticles containing at least one of Alcanivorax borkumensis, magnetic nanoparticles and metal elements containing at least one of platinum, silver, and lithium.

At this time, the reactant is a substance that specifically binds to a substance, and the bioelectrator can selectively detect only the reactant using the specific binding of the reactant.

At this time, the nanostructure may include a crosslinking agent or a precipitating agent in order to bind the reacted substance to the nanostructure.

The crosslinking agent may be selected from the group consisting of diisocyanate, dianhydride, diepoxide, dialdehyde, diimide, 1-ethyl-3-dimethylaminopropylcarbodiimide, glutaraldehyde, bis (imidoesters) Imidyl esters), and diacid chlorides.

At this time, the quenching agent may be selected from the group consisting of methanol, ethanol, 1-propanol, 2-propanol, butyl alcohol, acetone, polyethylene glycol, ammonium sulfate, sodium chloride, sodium sulfate, sodium phosphate, potassium chloride, potassium sulfate, May be used singly or in combination.

According to another aspect of the present invention, there is provided a floating structure including a water tank in which two liquids having different densities are not mixed with each other, and the above-described floating structure, which is located inside the water tank, Wherein the material to be reacted is formed to react with a reactant present in any one of the two liquids.

At this time, the floating structure When the liquid to be suspended contains an organic acid, the substance to be reacted may be lipase.

At this time, when the reactant includes at least one of a specific microorganism, a specific protein, and a specific DNA, the substance to be reacted includes at least one of an antibody, an antibody fragment comprising an antigen binding site of the antibody, and an aptamer .

The floating structure according to an embodiment of the present invention can support the material to be reacted including the body portion and can protect the material to be reacted for a long period of time from external environment change.

The floating structure according to an embodiment of the present invention includes the protrusions and the coupling grooves, so that the first body and the second body can be coupled without separating from the floating portion.

The floating structure according to an embodiment of the present invention includes a nanostructure that is a fixing member, so that the reaction target material can be fixed to the body part.

The floating structure according to an embodiment of the present invention may be formed to have a value between the density of two liquids so as to be floating at the interface of the first liquid and the second liquid which are two liquids.

In addition, the floating structure according to an embodiment of the present invention includes the first sub-fluid and the second sub-fluid to adjust the buoyancy in the state of being immersed in the first liquid and the second liquid, It is possible to allow floating.

The reactive material switching system having the floating structure according to an embodiment of the present invention facilitates the contact between the reactive material and the reactive material in a state where the floating structure floats on the interface between two liquids that are not mixed, The efficiency can be increased.

The floating structure according to an exemplary embodiment of the present invention can selectively detect only a specific substance using specific binding specific to a substance including a biomolecule.

1 is a schematic diagram showing a reactant conversion system having a suspended structure according to an embodiment of the present invention.
2 is a perspective view illustrating a floating structure according to an embodiment of the present invention.
3 is an exploded perspective view of a floating structure according to an embodiment of the present invention.
4 is a plan view showing a reaction material to be bonded to a fixing member of a floating structure according to an embodiment of the present invention.
5 is a cross-sectional view illustrating a floating portion of a floating structure according to an embodiment of the present invention.
6 is a cross-sectional view showing a first modified example of the floating structure floating unit according to an embodiment of the present invention.
7 is a cross-sectional view showing a second modified example of the floating structure floating unit according to an embodiment of the present invention.
8 is a cross-sectional view showing a third modified example of the floating structure body floating part according to an embodiment of the present invention.
FIGS. 9 to 11 are plan views illustrating a procedure for fabricating a floating structure according to an embodiment of the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, which will be readily apparent to those skilled in the art to which the present invention pertains. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and the same or similar components are denoted by the same reference numerals throughout the specification.

In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, parts, or combinations thereof. Also, where a section such as a layer, a film, an area, a plate, or the like is referred to as being "on" another section, it includes not only the case where it is "directly on" another part but also the case where there is another part in between. On the contrary, where a section such as a layer, a film, an area, a plate, etc. is referred to as being "under" another section, this includes not only the case where the section is "directly underneath"

Hereinafter, a floating structure according to an embodiment of the present invention will be described in detail with reference to the drawings.

1 is a schematic diagram showing a reactant conversion system having a suspended structure according to an embodiment of the present invention.

Referring to FIG. 1, a reactant conversion system 1 having a floating structure 2 according to an embodiment of the present invention may include a water tank 7 and a floating structure 2.

Meanwhile, in the embodiment of the present invention, the water tank 7 may contain two liquids having different densities without being mixed with each other.

At this time, in one embodiment of the present invention, the two liquids include a first liquid (3) and a second liquid (5) that do not interact, the second liquid having a density lower than that of the first liquid Can be located.

The floating structure 2 according to an embodiment of the present invention can support the reacted material 60 and can float on the interface of two liquids. At this time, the reacted material 60 may be formed to react with a reactant (not shown) present in any one of the two liquids.

The liquid in which the suspended structure 2 according to an embodiment of the present invention is suspended may be water containing an organic acid, and when the reactive substance is an organic acid, the reacted substance may be a lipase.

Meanwhile, in one embodiment of the present invention, the reacted material 60 may include, but is not limited to, a catalytic material or a biosensor.

Referring to FIG. 1, the organic acid present in the water, which is a reaction material, may react with a catalytic material supported on the body 10 of the suspended structure 2 to become a lipase.

1, the floating structure 2 according to an embodiment of the present invention can be formed by removing any reactant present in the liquid by the reacted material 60, You can switch.

Meanwhile, in one embodiment of the present invention, the reaction material may include at least one of a material having a specific binding specific to a material, for example, a specific microorganism, a specific protein, and a specific DNA. Thus, the biosensor can selectively detect only the reactant using the specific binding of the reactant.

2 is a perspective view illustrating a floating structure according to an embodiment of the present invention. 3 is an exploded perspective view of a floating structure according to an embodiment of the present invention.

Referring to FIG. 2, the floating structure 2 according to an embodiment of the present invention may include a body portion 10 and a floating portion 30. The floating structure 2 according to an embodiment of the present invention can support the material 60 to be reacted including the body portion 10 and can protect the material to be reacted for a long time from changes in the external environment.

Also, the floating structure 2 according to an embodiment of the present invention includes the floating portion 30 so that the body portion 10 can float on the interface between two liquids having different densities without mixing with each other.

The density of the floating structure 2 according to an embodiment of the present invention, that is, the sum of the density of the body portion 10 and the density of the floating portion 30 is greater than the density of the second liquid 5, ), The floating structure 2 according to an embodiment of the present invention can float on the first liquid.

At this time, the three-dimensional printer is manufactured so that the density of the suspended structure 2 according to the embodiment of the present invention is greater than the density of the second liquid 5 and smaller than the density of the first liquid 3, 2 liquid interface.

Referring to FIG. 3, the body 10 may include a first body 11 and a second body 21 in one embodiment of the present invention. At this time, the reacted material 60 is positioned between the first body 11 and the second body 21 so that the reacted material can be supported between the first body and the second body.

The first and second bodies 11 and 21 may be made of acrylonitrile-butadiene-styrene resin (ABS), polycarbonate (PC), polyvinyl alcohol (PVA), polystyrene (PS) ). ≪ / RTI >

In an embodiment of the present invention, the first body 11 and the second body 21 may have the same shape but may have a rectangular cross section. However, the present invention is not limited thereto. In addition, the first body 11 and the second body may have openings 13 and 23 formed therein so that the reactive material can approach from the outside to the inside.

Referring to FIG. 2, the first body 11 and the second body 21 are arranged in parallel to each other in the vertical direction, and each may be a planar structure in the form of a lattice.

Meanwhile, in an embodiment of the present invention, the second body 21 may be disposed adjacent to the surface of the first liquid 3. [ That is, for example, the second body 21 may be immersed in the first liquid 3 or floated on the first liquid 3 in contact with the first liquid 3.

In an embodiment of the present invention, the first body 11 and the second body 21 are separated into a single structure and are arranged in parallel with each other, but the present invention is not limited thereto and the first body and the second body may be integrally formed.

In an embodiment of the present invention, protrusions 15 and 25 may be formed at both ends of the body 10 to be coupled to the floating unit 30. [ However, the protrusions 15 and 25 of the body 10 may be inserted into the coupling grooves 33a and 37b of the floating portion 30, but the present invention is not limited thereto.

4 is a plan view showing a reaction material to be bonded to a fixing member of a floating structure according to an embodiment of the present invention.

Referring to FIG. 4, in an embodiment of the present invention, a fixing member 50 is positioned between the first body 11 and the second body 21, As shown in FIG.

At this time, the material to be reacted 60 may be fixedly coupled to the surface of the fixing member 50. In one embodiment of the present invention, the fixation member 50 may be a nanostructure, but is not limited thereto.

In an embodiment of the present invention, the first body 11 and the second body 21 are formed of a nanostructure in order to couple the reacted material 60 to the first body 11 and the second body 21, 1 body and the second body.

At this time, the body 10, that is, the first body 11 and the second body 21, may be made of polyacrylonitrile, nylon, polyester, polyurethane, polyvinyl chloride, polystyrene, cellulose, chitosan, A carbon nanotube, a carbon compound, a silica, a silica, a silica, a silica, a silica, a silica, a silica, a silica, a silicic acid, a polyacid, a polyacid, a polyglycolic acid, a polycarboxylic acid polycaprolactone, collagen, polypyrrole, polyaniline and poly Nanoparticles, and magnetic nanoparticles, and the reacted material may be directly bonded to the first body and the second body.

Meanwhile, in one embodiment of the present invention, a biomedical device may be an element capable of selectively detecting only a specific substance using a specific binding specific to a substance

In this case, the biomolecule may be an antibody, an antibody fragment including an antigen-binding site of the antibody, and an aptamer, but is not limited thereto, and may be a substance capable of selectively detecting only a specific substance using specific binding It is not limited to any substance.

Meanwhile, in one embodiment of the present invention, the catalytic material may include an enzyme comprising at least one of carbonic anhydrase, saccharide oxidase, lipase, acylase, lactonase, protease, peroxidase, But are not limited to, Acinetobacter calcoaceticus, Alcaligenes odorans, Aromatoleum aromaticum, Geobacter metallireducens, Dechloromonas aromatic, A magnetic nanoparticle containing at least one of Arthrobacter sp. And Alcanivorax borkumensis, and an organic and / or inorganic material containing at least one of metal elements including at least one of platinum, It may be an inorganic catalyst.

Meanwhile, in one embodiment of the present invention, the nanostructure is made of polyvinyl alcohol, polyacrylonitrile, nylon, polyester, polyurethane, polyvinyl chloride, polystyrene, cellulose, chitosan, polylactic acid, polylactic- Carbon nanotubes, carbon compounds and silica nanoparticles composed of at least one of glycolic acid, polyglycolic acid polycaprolactone, collagen, polypyrrole, polyaniline and poly (styrene-co-maleic anhydride) And may include at least one.

In one embodiment of the present invention, the nanostructure includes a functional group, and the functional group reacts with the substance 60 to be reacted, so that the substance to be reacted can be bonded to the nanostructure. At this time, depending on the reactant, the functional group to be reacted varies, so that the functional group can be specified depending on the reactant.

Meanwhile, in one embodiment of the present invention, the material to be reacted 60 may be bonded by being adsorbed on the nanostructure.

Also, in one embodiment of the present invention, a crosslinking agent or a precipitating agent may be included in the nanostructure in order to bind the reactant to the nanostructure.

In one embodiment of the present invention, the crosslinking agent is selected from the group consisting of diisocyanate, dianhydride, diepoxide, dialdehyde, diimide, 1-ethyl-3-dimethylaminopropylcarbodiimide, glutaraldehyde, bis ), Bis (succinimidyl ester), and diacid chloride.

In one embodiment of the present invention, the precipitating agent is selected from the group consisting of methanol, ethanol, 1-propanol, 2-propanol, butyl alcohol, acetone, polyethylene glycol, ammonium sulfate, sodium chloride, sodium sulfate, sodium phosphate, potassium chloride, Phosphate and an aqueous solution thereof may be used singly or in combination.

Referring to FIG. 2, the floating portion 30 may include a first floating body 31 and a second floating body 35 coupled to the body portion 10 in one embodiment of the present invention.

Referring to FIG. 3, the first fluid 31 may be coupled to the left end of the body 10, and the second fluid 35 may be coupled to the other end of the body, , It can be coupled to the right end.

5 is a cross-sectional view illustrating a floating portion of a floating structure according to an embodiment of the present invention. 6 is a cross-sectional view showing a first modified example of the floating structure floating unit according to an embodiment of the present invention. 7 is a cross-sectional view showing a second modified example of the floating structure floating unit according to an embodiment of the present invention. 8 is a cross-sectional view showing a third modified example of the floating structure body floating part according to an embodiment of the present invention.

5 to 8, in an embodiment of the present invention, the first sub-fluid 31 and the second sub-fluid 35 are in the form of a rectangular parallelepiped and have a fluid storage portion S May be formed.

5, the floating structure 30 according to one embodiment of the present invention can be suspended on the interface between the first liquid 3 and the second liquid 5, ) May be made of a three-dimensional printer so that the sum of the densities of the first liquid and the second liquid is larger than the density of the first liquid and smaller than the density of the second liquid.

5, the floating portion 30 is formed by calculating the density value so that the body portion 10 can float on the interface between the first liquid 3 and the second liquid 5 in advance . At this time, any shape may be employed if the sum of the densities of the floating portion 30 and the body portion 10 is greater than the density of the first liquid 3 and less than the density of the second liquid 5.

6 to 8, the floating structure 2 according to one embodiment of the present invention is immersed in two liquids so as to float on the interface between the first liquid 3 and the second liquid 5, which are two liquids It can move in the vertical direction.

6 to 8, in an embodiment of the present invention, the first fluid 31 and the second fluid 35 are arranged in the fluid reservoir S, The amount affects the buoyancy, so adjust the buoyancy by adjusting it.

The fluid reservoirs S of the first fluid 31 and the second fluid 35 are spaces in which the air and the liquid 3 and 5 are stored. It may be made of a material which does not cause an increase or decrease in volume.

In one embodiment of the present invention, the buoyancy control may be more complicated because the change in the volume of the first and third secondary fluids 31 and 35 must be taken into consideration.

That is, in one embodiment of the present invention, the first fluid 31 and the second fluid 35 are made of acrylonitrile-butadiene-styrene resin (ABS), polycarbonate (PC), polyvinyl alcohol (PVA) Polystyrene (PS), or the like.

Referring to FIG. 6, a first valve 39, a first pump 41, a second valve 43, and an air tank 45 may be disposed in the first sub-

At this time, as shown in FIG. 6, on the side surfaces of the first and third subfluids 31 and 35, the inflow ports 31a and 31b are formed on the left and lower ends of the first and second subfluids, 35a are formed in the right and left lower ends thereof, and discharge ports 31b, 35b are formed in the lower right end thereof so that the fluid can be discharged.

In one embodiment of the present invention, the first and third subfluids 31 and 35 are connected to the fluid (S) present in the fluid reservoir S through the inlets 31a and 35a and the outlets 31b and 35b Or liquid 3, 5).

The first valve 39 and the third valve 46 are installed in the first and second fluids 31 and 35 at one end and connected to the inlet ports 31a and 35a, And controls the flow path for flowing the fluid into the interior of the secondary fluid and the secondary fluid.

Referring to FIG. 6, when the first valve 39 is opened, the first liquid 3 and the second liquid 5 outside the first fluid 31 flow into the interior. Accordingly, the floating structure 2 according to an embodiment of the present invention can move downward by reducing buoyancy.

The second valve 43 and the fourth valve 49 are installed inside the first and second float 31 and 35 and connected to the outlets 31b and 35b at one end, And controls the flow path for discharging the fluid to the outside of the secondary fluid and the secondary fluid.

The other end of the second valve 43 and the fourth valve 49 are respectively connected to the first pump 41 and the second pump 47 so that the first auxiliary fluid 31 and the second auxiliary fluid 31, And moves the first liquid 3 and the second liquid 5, which are the fluids inside the two-part fluid 35, to the outside.

Referring to FIG. 6, when the first pump 41 operates to discharge fluid, the first liquid 35 and the second liquid 5, which are the fluids contained in the first fluid 31, 43, so that the amount of fluid in the first sub-fluid is reduced and the buoyancy of the first sub-fluid is raised.

At this time, the first pump 41 is a pump for discharging the fluid in one direction. However, the present invention is not limited to this, and the pump may be a pump for allowing fluid to flow in both directions. If the first pump 41 is a bi-directional pump, the first pump can be operated to allow fluid to flow through the first valve 39.

Meanwhile, the first pump 41 discharges the first liquid 3 and the second liquid 5, which are fluids in the first sub-fluid 31, to reduce the density of the first sub- Increases the buoyancy of the fluid.

Referring to FIG. 6, when the fluid is discharged by the first pump 41, the air tank 45 separates the first fluid 3 and the second fluid 5 from the first fluid 31 ) To increase the buoyancy of the first subfluid.

At this time, the air tank 45 may be an air cylinder tank and may be filled with compressed air. Further, the air tank 45 is effective to adjust the buoyancy when the first float 31 is in a position where air can not be obtained from the atmosphere.

Referring to FIG. 7, in an embodiment of the present invention, a fluid exists in the first sub-fluid 31 and a first pump 41 is provided outside the first sub-fluid, And may be connected to the left end of the first subfluid.

At this time, the first pump 41 may be a one-directional pump or a two-way pump, and the buoyant force may be controlled by introducing or discharging air or liquid 3, 5, which is fluid, into the first fluid 31.

Referring to FIG. 8, a first valve 39, a first pump 41, a second valve 43, and an air tank 45 may be disposed in the first fluid 31. At this time, as shown in FIG. 8, on the side surfaces of the first sub-fluid 31 and the second sub-fluid 35, outlets 31b, 31b are provided at the lower right end of the first sub- 35b are formed.

The first valve 39 and the third valve 46 are installed in the first and second fluids 31 and 35 and are connected to the air tank 45 at one end, And controls the flow path for flowing the fluid into the fluid and the second sub-fluid.

When the first valve 39 is opened in the embodiment of the present invention, compressed air having a high density inside the air tank 45 of the first sub-fluid 31 flows into the fluid reservoir S of the first sub- . Accordingly, the floating structure 2 according to an embodiment of the present invention can move downward by reducing buoyancy.

The other end of the second valve 43 is connected to the first pump 41 so that the air, which is the fluid inside the first subsidiary fluid 31, is moved to the outside by the pump dispensing operation.

Referring to FIG. 8, when the first pump 41 operates to send fluid, the fluid, which is the fluid inside the first fluid 31, may be discharged to the outside through the second valve 43.

Accordingly, the amount of the air in the first sub-fluid is reduced and the buoyancy of the first sub-fluid is increased, so that the floating structure 2 according to the embodiment of the present invention can move upward.

The operation principle of the first auxiliary fluid 31 is the same as that of the third valve 46, the second pump 47 and the fourth valve 49 of the second auxiliary fluid 35,

Referring to FIG. 2, the protrusions 33 and 37 may be formed in a rectangular parallelepiped shape in each of the first and second fluid 31 and 35 in one embodiment of the present invention.

At this time, the projections 33 and 37 are formed on one side where the first fluid 31 is coupled to the body 10, for example, on the right side or the left side where the second fluid 35 is coupled to the body 10 .

2, the protrusions 33 and 37 may be formed with coupling grooves 33a and 37b into which the protrusions 15 and 25 of the body 10 are inserted and fitted. At this time, the coupling grooves 33a and 37b may be formed so as to correspond to the protrusions so as to be fitted to the protrusions 15 and 25. [

9 to 11 are plan views illustrating a procedure for fabricating a floating structure according to an embodiment of the present invention.

The floating structure 2 according to an embodiment of the present invention can be manufactured by 3D printing. 9 and 10, a first body 11 and a second body 21, which are lattice-shaped planar structures, are manufactured by 3D printing.

At this time, the first body 11 and the second body 21 may have the same shape, and protrusions 15 and 25 may be formed at both ends.

11, a fixing member 50 coupled with an enzyme is coupled to the upper surface of the second body 21, and the reaction material 60 is placed between the first body and the second body by covering the first body 11, Lt; / RTI >

The protrusions 15 and 25 of the first body 11 and the second body 21 are connected to the coupling grooves 33a formed in the first float 31 and the second float 35, , 37b.

At this time, the floating structure 2 according to an embodiment of the present invention may be formed to have a value between two liquid densities so as to be floating at the interface of the first liquid 3 and the second liquid 5, which are two liquids.

The floating structure 2 according to an embodiment of the present invention includes a first fluid 31 and a second fluid 35 and is immersed in the first liquid 3 and the second liquid 5 It is possible to float the interface between the first liquid and the second liquid by adjusting the buoyancy.

At this time, in one embodiment of the present invention, the buoyant force can be adjusted by adjusting the amount of air present in the fluid reservoir of the first reservoir 1, and the first liquid 3 and / The buoyant force can be adjusted by adjusting the amount of the second liquid 5.

That is, when the floating structure 2 according to an embodiment of the present invention is positioned below the interface between the two liquids, the air existing in the fluid storage portion S of the first and second sub- Or the first liquid (3) and the second liquid (5) are discharged so as to be made smaller in density so as to be positioned at the interface of the two liquids.

In addition, when the floating structure 2 is positioned above the interface of the two liquids, the second liquid 5 existing in the fluid storage portion S of the first fluid 31 and the second fluid 35 flows The density is increased so that it is located at the interface of the two liquids.

Accordingly, the floating structure 2 according to an embodiment of the present invention is capable of performing material separation in a mixture, separation of enantiomers and interfacial reaction through catalytic reaction by a substance to be reacted bonded to a fixing member 50 Lt; / RTI >

The floating structure according to an embodiment of the present invention can support the material to be reacted including the body portion and can protect the material to be reacted for a long period of time from external environment change.

The floating structure according to an embodiment of the present invention includes the protrusions and the coupling grooves, so that the first body and the second body can be coupled without separating from the floating portion.

The floating structure according to an embodiment of the present invention includes a nanostructure that is a fixing member, so that the reaction target material can be fixed to the body part.

The floating structure according to an embodiment of the present invention may be formed to have a value between the density of two liquids so as to be floating at the interface of the first liquid and the second liquid which are two liquids.

In addition, the floating structure according to an embodiment of the present invention includes the first sub-fluid and the second sub-fluid to adjust the buoyancy in the state of being immersed in the first liquid and the second liquid, It is possible to allow floating.

The reactive material switching system having the floating structure according to an embodiment of the present invention facilitates the contact between the reactive material and the reactive material in a state where the floating structure floats on the interface between two liquids that are not mixed, The efficiency can be increased.

The floating structure according to an exemplary embodiment of the present invention can selectively detect only a specific substance using specific binding specific to a substance including a biomolecule.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

1: Reactant Conversion System 2: Floating Structure
3: first liquid 5: second liquid
7: Water tank 10: Body part
11: first body 13, 23: opening
15, 25: protrusion 21: second body
30: Floating portion 31: Part 1 Fluid
33, 37: projections 33a, 37a:
35: second fluid 39: first valve
41: first pump 43: second valve
45: Air tank 46: Third valve
47: second pump 49: fourth valve
50: fixing member 60: substance to be reacted

Claims (27)

A body portion for supporting the reaction target material; And
And at least one floating portion coupled to the body portion to allow the body portion to float on an interface between two liquids having different densities,
Wherein the material to be reacted is formed to react with a reactive substance present in any one of the two liquids. The method according to claim 1,
Wherein the two liquids comprise a first liquid that does not interact and a second liquid that is less dense than the first liquid and that is located on the first liquid, wherein the sum of the body portion and the floating portion density is greater than the second Wherein the first liquid is suspended on the first liquid, the second liquid being larger than the density of the liquid and less than the density of the first liquid. 3. The method of claim 2,
The body
A first body and a second body,
Wherein the reaction target material is positioned between the first body and the second body so as to support the reaction target material. The method of claim 3,
Wherein each of the first body and the second body has an opening through which the reactive material can approach from the outside to the inside. The method of claim 3,
Wherein each of the first body and the second body is a lattice type planar structure. 6. The method of claim 5,
Wherein the first body and the second body are arranged in parallel to each other in the vertical direction, and the second body is disposed adjacent to the surface of the first liquid. 6. The method of claim 5,
Wherein the first body and the second body are formed in the same shape. The method of claim 3,
Wherein the first body and the second body are made of a material selected from the group consisting of polyacrylonitrile, nylon, polyester, polyurethane, polyvinyl chloride, polystyrene, cellulose, chitosan, polylactic acid, polylactic- A suspension structure formed of at least one of a nanofiber, a carbon nanotube, a carbon compound, a silica nanoparticle, and a magnetic nanoparticle composed of at least one of polycaprolactone, collagen, polypyrrole, polyaniline and poly (styrene- . The method of claim 3,
And a fixing member positioned between the first body and the second body,
Wherein the material to be reacted is fixed to a surface of the core member. 10. The method of claim 9,
Wherein the fixing member is a nano structure. The method according to claim 1,
The floating portion
A first body fluid coupled to one end of the body portion and a second fluid fluid coupled to the other end of the body portion,
Wherein the first float and the second float allow the body to be moved up and down while being immersed in the two liquids so as to float on the interface of the two liquids. The method according to claim 1,
Wherein the first sub-fluid or the second sub-fluid is formed with a fluid storage portion which is a space in which a fluid can be stored,
And a pump connected to the first sub-fluid or the second sub-fluid to cause the fluid to flow in and out of the fluid reservoir. 13. The method of claim 12,
Wherein the fluid flowing into and out of the fluid reservoir through the pump is air or the two liquids. 12. The method of claim 11,
Protruding portions are formed at both ends of the body portion,
Wherein each of the first sub-fluid and the second sub-fluid has an engaging groove coupled to the protrusion. 11. The method of claim 10,
Wherein the body portion and the floating portion are formed of at least one polymer material selected from acrylonitrile-butadiene-styrene resin (ABS), polycarbonate (PC), polyvinyl alcohol (PVA), and polystyrene (PS). 16. The method of claim 15,
The nanostructure may be at least one of polyvinyl alcohol, polyacrylonitrile, nylon, polyester, polyurethane, polyvinyl chloride, polystyrene, cellulose, chitosan, polylactic acid, polylactic-co- A suspension structure comprising at least one of nanofibers, carbon nanotubes, carbon compounds, silica nanoparticles, and magnetic nanoparticles made of at least one of lactone, collagen, polypyrrole, polyaniline and poly (styrene-co-maleic anhydride). 11. The method of claim 10,
Wherein the nanostructure comprises a functional group, and the functional group reacts with the substance to be reacted, whereby the substance to be reacted is bound to the nanostructure. 11. The method of claim 10,
Wherein the substance to be reacted is bound to the nanostructure by being adsorbed on the nanostructure. The method according to claim 1,
Wherein the substance to be reacted comprises a catalytic material or a biomolecule. 20. The method of claim 19,
The catalyst material
An enzyme comprising at least one of carbonic anhydrase, glucose oxidase, lipase, acylase, lactonase, protease, and peroxidase, an enzyme comprising at least one of Acinetobacter calcoaceticus, Alcaligenes odorans, Aromatoleum aromaticum, Geobacter metallireducens, Dechloromonas aromatic, Arthrobacter sp., Alkali borax borcumen, Wherein the microcapsule is at least one selected from the group consisting of microorganisms including at least one of cesium (Alcanivorax borkumensis), magnetic nanoparticles, and metallic elements containing at least one of platinum, silver and lithium. 20. The method of claim 19,
The reactant is a substance which has a specific binding specific to a substance,
Wherein the biomolecule selectively detects only the reactant using specific binding of the reactant. 11. The method of claim 10,
Wherein the nanostructure includes a crosslinking agent or a precipitating agent for binding the reacted substance to the nanostructure. 23. The method of claim 22,
The cross-
(Imidoesters), bis (succinimidyl esters), and di (diisocyanates), diisocyanates, dianhydrides, diepoxides, dialdehydes, diimides, 1-ethyl-3-dimethylaminopropylcarbodiimide, glutaraldehyde, bis Wherein the at least one compound is at least one compound selected from the group consisting of an acid chloride and an acid chloride. 23. The method of claim 22,
The quasi-
Solely or as a mixture of methanol, ethanol, 1-propanol, 2-propanol, butyl alcohol, acetone, polyethylene glycol, ammonium sulfate, sodium chloride, sodium sulfate, sodium phosphate, potassium chloride, potassium sulfate, potassium phosphate and their aqueous solutions Floating structure. A water tank in which two liquids having different densities are accommodated without being mixed with each other; And
A floating structure according to any one of claims 1 to 24, which is located inside the water tank, supports the material to be reacted and is floating at the interface of the two liquids,
Wherein the material to be reacted is formed to react with a reactant present in any one of the two liquids. 26. The method of claim 25,
Wherein when the liquid in which the floating structure is suspended comprises an organic acid, the substance to be reacted is lipase. 26. The method of claim 25,
When the reaction material comprises at least one of a specific microorganism, a specific protein and a specific DNA, the substance to be reacted includes at least one of an antibody, an antibody fragment comprising an antigen binding site of the antibody, and an aptamer Reactant conversion system.
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