KR20170000840A - A polysaccharide having improved immuno-activity from Enteromorpha prolifera and preparation method thereof - Google Patents
A polysaccharide having improved immuno-activity from Enteromorpha prolifera and preparation method thereof Download PDFInfo
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- KR20170000840A KR20170000840A KR1020150089471A KR20150089471A KR20170000840A KR 20170000840 A KR20170000840 A KR 20170000840A KR 1020150089471 A KR1020150089471 A KR 1020150089471A KR 20150089471 A KR20150089471 A KR 20150089471A KR 20170000840 A KR20170000840 A KR 20170000840A
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- polysaccharide
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- supernatant
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/324—Foods, ingredients or supplements having a functional effect on health having an effect on the immune system
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- A—HUMAN NECESSITIES
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- A23V2250/00—Food ingredients
- A23V2250/50—Polysaccharides, gums
- A23V2250/51—Polysaccharide
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract
The present invention relates to a polysaccharide derived from a visa-derived polysaccharide having enhanced immunological activity and a method for producing the polysaccharide. The present invention relates to a polysaccharide derived from a visa-derived polysaccharide having improved immunity, Derived polysaccharide obtained in the present invention has an excellent effect of providing a functional food composition for immunity enhancement.
Description
The present invention relates to a polysaccharide derived from a visa-derived polysaccharide having enhanced immunological activity and a method for producing the polysaccharide. More particularly, the present invention relates to a polysaccharide derived from a polysaccharide having enhanced immunological activity, The present invention relates to a process for producing a polysaccharide derived from a visa-derived polysaccharide.
Seaweeds are a group of birds in the sea, and are botanically classified into four groups of plants: green algae, brown algae, red algae, and southern algae. They contain chlorophyll a as a common chlorophyll, but b contained in higher plants are contained only in green algae and not in other algae (Nutrition Dictionary, March 15, 1998, Academy Book). The green algae are green, the brown algae are kelp and the seaweed, and the red algae is green (traditional Korean food, September 5, 2005). Seaweed has not only been used for edible purposes, but has also been used for feed, food additives, fertilizers, and so on. Fifty of the 500 species of seaweed in Korea are used for food (Powerfood Superfood, 2010.12.11, Blue Happiness), but in other countries such as Europe, raw materials of food additives such as alginic acid, carrageenan and agar, Etc., and a very small portion is used for food.
Enteromorpha prolifera is a marine algae of green algae. It is green or pale green in color and is 10 ~ 30cm high. Its shape and size are very diverse, sometimes reaching several meters. The whole body is tubular, with a little flattened side, with many side branches, and side branches give small branches again. The end of the branch is not a single cell, and the length of the cell is 10-12 μm when viewed from the surface. In the young part, the cells are arranged vertically and become irregular after they are all grown. The distribution area is distributed all over Korea and all over the world. The habitat is rich in nutrient and pollution-free river mouth due to the inflow of inner bay or fresh water. It grows mainly on rocks, dead branches or other seaweeds. The main production area of our country is Pusan Gadeokdo, Gyeongnam Sacheon, Jeonnam Jangheung and Muan. It is usually harvested 4 to 6 times from the habitats that form natural communities using hands and / or collectors. It is rich in minerals and vitamins, and has a unique flavor and taste. It is also used as an additive for jams, soups and confectionery. Although exporting more than 80t per year, the total amount depends on natural products. Recently, it has started to cultivate because of environmental pollution, and it has become more popular as a new breed of cultivar because it is easy to cultivate, easy to cultivate, easy to harvest and easier to collect than other seaweeds. (Green laver) ).
Visible parasites are rich in iron, potassium, iodine, inorganic salts, vitamin A, and vitamin C, which clears blood and contains nutrients. It is also known to be effective in neutralizing various toxic components of the human body and detoxifying nicotine. It is known to be effective in activating liver diseases, hangover, blood flow cells, and is rich in vegetable fiber, And colonic peristaltic movements to help the constipation has an excellent effect, which contains a large amount of ingredients to calm the inflammation of the body, has been reported to be effective in arthritis and muscle pain.
The present invention relates to a method for extracting a water-soluble component by adding distilled water to Korean poultry and stirring the mixture and centrifuging the mixture to obtain a supernatant, or a method in which ammonium sulfate is added to the supernatant to precipitate a protein component, (Korean Patent No. 10-0617495) discloses that a lectin component can be effectively used as an agent for enhancing immunity that can effectively activate immunity without cytotoxicity to normal cells.
As a basic study for the efficient utilization of visible parasitoids cultured in Korea, the general components of visible parasites were analyzed, and water-soluble polysaccharides were extracted and fractionated to determine their chemical characteristics (2002) Extraction and chemical composition of water-soluble polysaccharide from barnyardgrass, Journal of Fisheries Science, 35 (5), 519-523).
The present invention relates to a process for the production of a food using an extract of an antioxidant and a method for producing the same, It is characterized by having an activity of suppressing NO production, inhibiting TNF-α production, inhibiting IL-6 production, inhibiting the production of IL-1β and inhibiting prostaglandin E 2 production Korean Patent No. 10-1110061 discloses an anti-inflammatory agent composition comprising a barnyardgrass extract. It has also been disclosed that it can be effectively used for the prevention and treatment of diabetes, a chronic disease, and thus can be used as an anti-diabetic functional food, concerning the use of a functional dietary food and a visa palatable containing an extract having an anti-diabetic function (Korean Patent Laid-Open No. 10-2010-0001635), Korean Patent No. 10-1179101 related to polysaccharides derived from algae having immunity enhancing activity and a method for producing the same, sequentially treats an organic solvent in a green alga, (TNF-α), interleukin 1β (IL-1β), and prostaglandin E 2 (PGE 2 ) of the produced polysaccharide. As a result, it was confirmed that the polysaccharide derived from the parasitoid was effective in secreting the immuno-enhancement-related cytokine, This can be used as a composition for immunity enhancement that may have been disclosed. However, none of the above documents discloses a method for producing polysaccharides derived from visa-derived polysaccharides treated with an organic acid in the extraction of polysaccharides derived from visa parasites in which the activity of immune cells is enhanced.
Accordingly, an object of the present invention is to provide a method for producing a polysaccharide derived from a visa-derived polysaccharide with enhanced immunological activity.
Another object of the present invention is to provide a food composition for enhancing immunostaining which contains polysaccharides derived from visible light.
The above object of the present invention can be accomplished by a method for producing a dried fruit of the present invention, comprising: drying a washed visa leaf after drying; adding 80% alcohol to the powdered visa leaf to remove the pigment component and filtering; Adding the distilled water to the visa pulp from which the pigment is removed in the above step, treating the citric acid to extract the polysaccharide component, and then centrifuging to recover only the supernatant; The supernatant recovered in the above step is concentrated under reduced pressure, and then added to the concentrated supernatant so as to be 70% (v / v) of the alcohol, followed by centrifuging to remove the supernatant and to obtain the remaining precipitate; Removing the remaining alcohol in the precipitate obtained in the step; The polysaccharide obtained in the above step is lyophilized and then pulverized to prepare a polysaccharide derived from the present invention, and the effect of enhancing the immune activity of the polysaccharide derived from the present invention is confirmed, .
The present invention has an excellent effect of providing a method for producing a polysaccharide derived from a visa-derived polysaccharide with enhanced immunological activity, and further has an excellent effect of providing a functional food composition for enhancing immunity activity containing a polysaccharide derived from a visa-derived polysaccharide as an active ingredient.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a diagram showing a method for producing a polysaccharide derived from a visa-like plant according to Example 1 of the present invention. FIG.
2 is a graph showing the results of measurement of the NO production ability of the polysaccharide derived from visas parasites according to Experimental Example 2 of the present invention.
3 is an HPSEC chromatogram of Sulfuric Acid Polysaccharide Derived from Visible Parasites according to Citric Acid Treatment Time of Experimental Example 3 of the present invention.
FIG. 4 is a graph showing the results of measurement of mitogenic activity of immature cells of Sulfur-containing polysaccharides derived from visible light according to citric acid treatment time in Experimental Example 4 of the present invention.
5 is a graph showing the results of measurement of the NO production ability of the sulfated polysaccharides derived from visible light according to the citric acid treatment time in Experimental Example 4 of the present invention.
6 is a graph showing the results of measurement of the NO production ability according to the concentration of Sulfuric Acid Polysaccharide derived from Visible Parasites according to Experimental Example 4 of the present invention.
Fig. 7 is a diagram of a process for producing a polysaccharide derived from a visa-derived polysaccharide to which an organic acid low molecular compounding method is added to Example 1 of the present invention.
The present invention discloses a polysaccharide derived from a visa-derived polysaccharide having enhanced immunological activity and a method for producing the polysaccharide.
Hereinafter, the present invention will be described in more detail by way of examples. However, the following examples are intended to illustrate the invention and are not intended to limit the scope of the invention.
[Example 1] Dispersion material of the present invention and polysaccharide extracted from visible light
In the present invention, barnyardgrass was purchased from domestic farming / local fishery farming corporation, washed with water, dried and pulverized and used as a disclosure material. As shown in FIG. 1, 500 kg of 80% alcohol was added to 10 kg of pulverized dried powder, and the mixture was reacted at 75 ° C for 1 hour. The pigment components were removed and filtered to remove impurities. The visually-pulsed samples obtained above were placed in a stirrer and a constant of 20 times as much as the weight of the sample was added thereto. The pump and the lines were connected at 65 ° C for 4 hours, followed by stirring and circulation-extraction. / hr. After centrifugation for 3 hours, the sludge and the extract were separated. The separated extract was concentrated to twice the volume using a vacuum concentrator. To the concentrated extract, the alcohol was added so that the total alcohol content was 70% (v / v), the mixture was stirred at 1,300 rpm for 5 minutes with a homomixer, stored at 4 ° C for 24 hours, centrifuged at 15,000 rpm using a tubular centrifuge The supernatant was removed and the remaining precipitated material was freeze-dried after removing the alcohol. The freeze-dried polysaccharide originated from the freeze-dried polysaccharide was pulverized and powdered. The polysaccharide derived from the powdered polysaccharide was referred to as crude polysaccharide.
[Example 2] Purification of polysaccharides by ion exchange column chromatography of the present invention
In this example, an ion exchange resin (DIAION HP-20) was charged in the column to separate the polysaccharide extracted from the visa parasites according to the degree of ionization. 100 mg of the crude polysaccharide obtained in Example 1 was dissolved in 10 mL of distilled water and heated in a microwave oven for 30 seconds using a microwave bomb. The mixture was filtered at 3.0 and then injected into a column to give a mobile phase of 0 to 1.5 M NaCl at a flow rate of 1.5 mL / min. The purified polysaccharide was injected into a dialysis membrane of Mw 8000, dialyzed in normal water for 2 days and distilled water for 1 day, and then lyophilized under reduced pressure to obtain a final purified polysaccharide derived from Pseudomonas sp. The thus obtained polysaccharide was referred to as a sulfur-containing polysaccharide.
[Experimental Example 1] Measurement of the total carbohydrate content and general components of the polysaccharide derived from the visa-derived polysaccharide of the present invention
The total carbohydrates and the general component contents of the polysaccharides derived from the visas of the present invention obtained in Examples 1 and 2 were measured. Protein, Sulfate, Uronic acid, Monosaccharide (L-Rhamnose, D-Xylose, D-Glucose)
The total carbohydrate content was determined by adding phenol to the obtained polysaccharide, adding sulfuric acid to decompose the polysaccharide into monosaccharides, reacting the phenol with the decomposed monosaccharide to make it colored, measuring the absorbance of the Rhamnose standard at 490 nm The total carbohydrate content of polysaccharides was measured.
Protein content was determined by measuring the absorbance at 570 nm after reacting Reagent A and Reagent B in the dark room with the Lowry method using Bovine serum albumin as a standard.
The content of sulfate group was then measured by the barium chloride Gelatine-turbidimetric method, and the content of the Uronic acid is hydrolyzed for 2 hours in H 2 SO 4, was added 3,5-dimethylphenol Scott et al. (1984).
The monosaccharide was dissolved in Trifluoroacetic acid (TFA), hydrolyzed at 121 ° C for 2 hours, neutralized with NaOH, and analyzed by Carbohydrate analysis column (Waters, USA). Elution conditions were flow rate; 2.0 L / min, Eluent; 90% acetonitrile solution was used. The glucose, fructose, mannose, galactose and xylose solutions were measured as standard solutions, respectively, compared with the monosaccharides of the sample solutions.
As shown in Table 1, the total carbohydrate content of the polysaccharide obtained in Example 1 was 61.6%, the protein content was 4.5%, the sulfate content was 23.9%, and the uronic acid content was 19.4%. On the other hand, the monosaccharide constituting the carbohydrate was mainly L-Rhamnose (64.6%), and a considerable amount of D-Glucose (18.8%) and D-Xylose (16.6%) were added. Also, the polysaccharide obtained in Example 2 had a total carbohydrate content of 47.2 to 48.6%, a protein content of 11.0 to 12.1%, a sulfate content of 25.2 to 26.6% and a uronic acid content of 14.8 to 15.1%, and the monosaccharide constituting the carbohydrate It was determined that L-Rhamnose contained 58.7 ~ 60.1% and D-Xylose (15.3 ~ 16.1%) and D-Glucose (24.0 ~ 26.0%) were contained. It can be seen that this is totally different from the components disclosed in Korean Patent No. 10-0617495.
[Experimental Example 2] Measuring effect of enhancing immunity activity of polysaccharide derived from visipera of the present invention
In this Experimental Example, the effect of enhancing the immunoreactivity of Purified Sulfuric Acid Polysaccharide derived from Purified Purified Purified Sulfuric Acid in Example 2 was measured by NO production ability. Mouse macrophages used Raw 264.7 cells. Raw 264.7 cells were cultured at 10, 50, 100 and 200 ㎍ / mL concentration of Sulfuric Acid Polysaccharide. After culturing for 24 hours, Griess reagent was added to 100 μL of the culture supernatant, and incubated for 10 minutes at room temperature. The absorbance at 540 nm was measured and the concentration of nitrite, the metabolite of NO, was calculated from the standard curve obtained using sodium nitrite. Lipopolysaccharide (LPS) was used as a control.
As shown in FIG. 2, the sulfhydryl polysaccharide in Raw 264.7 cells at a concentration of 10-200 ㎍ / mL showed a slight NO production ability of <5 ㎍ / mL at all concentrations except for the second sample. These results confirm that the NO production ability of the sulfated polysaccharide extracted by the above Example 2 is not so large.
In recent studies, it has been reported that the immunopotentiating activity of the immune cells of Sulfuric acid polysaccharide is closely related to the structure of polysaccharide molecules (Kangnung Wonju National University, thesis, Mehdi Tabarsa, And structure-activity association). Among the main factors, the decrease in molecular weight was related to the relative molar ratio of the sulfated polysaccharide reacting with the immune cells, and the molecular weight of the sulfated polysaccharide , And have been shown to be effective in enhancing the activity of immune cells very efficiently. Therefore, in order to increase the activity of immune cells by decreasing the molecular weight of the sulfuric polysaccharide, citric acid, which is one kind of organic acid, is treated with sulfuric acid polysaccharide to decrease the molecular weight and to decrease the molecular weight, [Experimental Example 4].
[Experimental Example 3] Measurement of molecular weight and molecular size by the citric acid treatment and the citric acid treatment of the sulfuric acid polysaccharide derived from Visafalam
In this Experimental Example, citric acid treatment and molecular weight were measured on the sulfhydryl-derived polysaccharide derived from Vasopalas obtained in Example 2.
First, the citric acid treatment of Sulfuric Acid Polysaccharide Derived from Visible Paraffin was carried out by dissolving 15 g of Sulfuric Acid Polysaccharide derived from Visible Paraffin in 5 mL of 0.1 M citric acid, heating at 70 ° C for 30 minutes, 60 minutes, 120 minutes and 180 minutes respectively and then neutralizing with 1 M NaOH Respectively. After neutralization, dialysis and freeze-drying were conducted to measure the molecular weight.
The HPSEC-MALLS-RI system was used to measure molecular weight and molecular size. The citric acid-treated sphalerite polysaccharide was dissolved and filtered through 3.0 쨉 m membrane and loaded onto a high performance size exclusion chromatography-multiangle laser light scattering-refractive index detector (HPSEC-MALLS-RI) system. The HPSEC-MALLS-RI system consists of three SEC columns (TSK G5000 PW (7.5 mm × 600 mm), TSK G3000 PWxl and TSK G2500 PWxl (7.8 mm × 300 mm), TosoBiosep) and a multiangle laser light scattering detector (HELEOS, Wyatt Technology Corp., Santa Barbara, CA, USA) and Refractive Index Detector (RI-150, Thermo Electron Corp., Yokohama City, Japan). Elution conditions are as follows: Flow rate; 0.4 mL / min, Eluent; 0.15M Na-Nitrate. Molecular weight (Mw) and molecular size (Rg) were calculated from ASTRA 5.3 software from data obtained from MALLS and RI.
As a result, the HPSEC chromatogram of the sulfuric polysaccharide treated with citric acid over time showed that the sulfuric polysaccharide molecules not treated with citric acid eluted at 25 to 53 minutes from the SEC column as in [FIG. 3] Polysaccharide molecules eluted around the peak at 32.5 min and appeared to elute small amounts of molecules at very small peaks around 45 min. Sulfuric acid polysaccharides treated with citric acid for 30 minutes showed similar elution times with those of non-citric acid-treated sulfuric polysaccharides. The main peak appeared at 32.5 min, but a small shoulder peak appeared at 40 min. The citric acid treatment seemed to break down enormous molecules and elute them. Sulfuric acid polysaccharide treated with citric acid for one hour showed similar main peak to sulphate polysaccharide treated with citric acid and sulphate polysaccharide treated with 30 min. However, the peak at 40 min was significantly larger than the sulphated polysaccharide treated at 30 min And the decomposition of the molecules was more advanced. On the other hand, citric acid polysaccharide treated with citric acid for 120 minutes and 180 minutes showed significant shift of the main peak (about 35 minutes) and the size of the second peak after 40 minutes was considerably larger. Showed significant breakdown of the molecules. The measurement results of the molecular weight and the molecular size of the sulfated polysaccharide by citric acid treatment are shown in [Table 2]. As shown in [Table 2], the major peak of 1,247 × 10 3 g / mol of citrate-free polysaccharide was 443 × 10 3 g / mol. The molecular weights of the sulfated polysaccharides treated for 30 min and 60 min were 502.8 × 10 3 g / mol and 429 × 10 3 g / mol for the major peaks, 143.7 × 10 3 g / mol and 136 × 10 3 g / mol for the small peaks, Respectively. On the other hand, in the 120 minutes, the shift of the peak occurs, the hwangham polysaccharide processed 180 minutes the molecular weight was measured by a further decrease 398.8 × 10 3 g / mol to 353.8 × 10 3 g / mol, a small peak 99.9 × 10 3 g / mol and 29.5 × 10 3 g / mol, respectively. In this experiment, it was found that significant decomposition took place in the sulfuric acid dihydrate treated with organic acid citric acid over time.
[Experimental Example 4] Measuring effect of enhancing immune activity by treatment with Citric Acid of Sulfur-derived polysaccharide
In the present Experimental Example 3, the immunopotentiating activity of citric acid polysaccharide treated with citric acid over time was confirmed by measurement of mitogenic activity and measurement of NO production ability on immune cells.
(1) Measurement of mitogenic activity on immune cells
Mitochondrial activity of immune cells was examined using mouse macrophage (Raw 264.7) cells. Raw 264.7 cells were plated at a density of 1 × 10 6 cells / well on a 96-well plate and cultured in an incubator at 37 ° C. and 5% CO 2 for 24 hours. In Experimental Example 3, the concentration of sulfuric polysaccharide treated with
As a result, as shown in FIG. 4, the citric acid-free sulfated polysaccharide did not significantly affect the proliferative activity of RAW 264.7 cells at the concentration of the experiment. However, at the highest concentration (200 ㎍ / mL), about 120% of the cells were able to proliferate. However, the citric acid - treated saururus polysaccharide exhibited significantly higher RAW 264.7 cell proliferation regardless of treatment time. In addition, a significant increase in RAW 264.7 cell proliferation was observed at a concentration of 10 μg / mL. Therefore, it was found that when low - molecular weight Sulfuric acid polysaccharide was lowered by citric acid, low molecular weight Sulfuric acid polysaccharide had better ability of RAW 264.7 cell proliferation. In addition, the results showed that the low molecular weight red ginseng polysaccharide did not show cytotoxicity to RAW 264.7 cells.
(2) Measurement of NO production ability
The NO production ability measurement of this experimental example was measured by the method of Experimental Example 2 above. As a result, as shown in Fig. 5, the sulfur-containing polysaccharides not treated with citric acid showed a remarkably weak NO production ability at almost all concentrations. On the other hand, citric acid polysaccharide treated with citric acid for 30 minutes was found to have a considerable amount of NO production ability even at a concentration of 10 μg / mL. As the concentration of sulfuric polysaccharide increased, the NO production capacity also increased. However, at a concentration of 200 ㎍ / mL, the NO production capacity is slightly decreased, and the maximum dose is preferably less than 200 ㎍ / mL. Sulfuric polysaccharides treated at 60 and 120 min showed a NO production pattern very similar to that of 30 min. On the other hand, Sulfuric acid polysaccharide treated for 180 min showed relatively increased NO production ability as compared with other sulfur polysaccharides. From the above results, it was found that the treatment concentration of sulfuric polysaccharide was considerably important. When RAW 264.7 cells were treated at a slightly lower concentration in the experimental group, the NO production ability was examined. As a result, citric acid treated saururus polysaccharides Regardless of the treatment time, the NO generation ability was found to be substantially similar (Fig. 6). This was significantly different from the NO production performance disclosed in Korean Patent No. 10-1179101.
Thus, as can be seen from the results of Experimental Example 4, when polysaccharides derived from visually-pollinated polysaccharides are added to citric acid, which is an organic acid, by a low-molecular-weight method, the stimulation effect of the immune cells is enhanced It was shown that the polysaccharide-derived polysaccharide was increased by adding an organic acid low molecular weight method in Example 1 (FIG. 7).
The Sulfur-containing polysaccharide of the present invention having the immunopotentiating effect according to the present invention may have conventional formulations and may be in the form of tablets, capsules, rings, granules, liquids, powders, flakes, pastes, syrups, gels, ≪ / RTI > The composition of each of these formulations may contain various kinds of bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art. Hereinafter, when the effect of enhancing the immune activity is excellent, a granule and a liquid composition are exemplified as a typical application example of a food composition.
[Formulation Example 1] Preparation of granules containing the polysaccharide derived from Visafalala of the present invention
Examples of the granules containing the polysaccharides derived from the visa-derived polysaccharides prepared in the present invention are as shown in Table 3, in which a mixture prepared from polysaccharides derived from visible-ray-derived polysaccharides is supplied to the granulator at a constant speed, -in weight feeder), the supplied powder moves by its own vibration, water of a certain temperature is injected by the compressed air of the nozzle at the upper part of the powder layer, and hot air is supplied at the bottom of the powder, And the supplied hot air was discharged to the outside through the air filter installed on the upper part. When the granulated particles were discharged to the outlet, they were passed through a granule of 40 mesh to prepare granules.
[Formulation Example 2] Preparation of a liquid phase containing polysaccharides derived from visible light of the present invention
Table 4 shows the formulation examples of the liquid phase containing the polysaccharide derived from visa parasites prepared in the present invention. The liquid form of the liquid form was prepared at the composition ratios as shown in [Table 4]. The emulsifier used in [Formulation Example 2] was prepared by adding 0.5 to 5% of a fatty acid ester of sorbitan and a fatty acid ester of glycerin to obtain a liquid form of a liquid form so that the total solid content of the product was 20.5%.
The present invention has an effect of providing a polysaccharide having an enhanced immunosuppressive activity derived from visible light and has an excellent effect of providing a food composition containing a polysaccharide derived from visible light as an active ingredient and thus is very useful as a health functional food industry It is an invention.
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|---|---|---|---|---|
| KR20190078182A (en) * | 2017-12-26 | 2019-07-04 | 동신대학교산학협력단 | Composition for Removing Hangover Using an Extract of Enteromorpha prolifera, Sprout Ginseng etc. |
| CN111333743A (en) * | 2019-12-04 | 2020-06-26 | 青岛科技大学 | Decolorizing process method of enteromorpha polysaccharide |
| CN115679689A (en) * | 2022-12-12 | 2023-02-03 | 杨开健 | Flame-retardant fabric finishing agent and fabric finishing method |
| CN118063643A (en) * | 2024-04-18 | 2024-05-24 | 康源领鲜科技有限公司 | Enteromorpha polysaccharide extraction process and application thereof in salmon feed |
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| KR101949885B1 (en) | 2017-08-02 | 2019-02-19 | 주식회사 비케이바이오 | Polysaccharide obtained from red cabbage with immune-enhancing activity, anti-tumor activity and method for producing the same |
| KR102429824B1 (en) | 2020-08-03 | 2022-08-05 | 충남대학교산학협력단 | Composition for Prophylaxis or Treatment of Cognitive Dysfunction Comprising Enteromorpha Prolifera |
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| KR20030020334A (en) * | 2003-01-30 | 2003-03-08 | 배태진 | Manufacturing process of functional helth beverage using fucoidan from sea mustard |
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| KR20030020334A (en) * | 2003-01-30 | 2003-03-08 | 배태진 | Manufacturing process of functional helth beverage using fucoidan from sea mustard |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190078182A (en) * | 2017-12-26 | 2019-07-04 | 동신대학교산학협력단 | Composition for Removing Hangover Using an Extract of Enteromorpha prolifera, Sprout Ginseng etc. |
| CN111333743A (en) * | 2019-12-04 | 2020-06-26 | 青岛科技大学 | Decolorizing process method of enteromorpha polysaccharide |
| CN115679689A (en) * | 2022-12-12 | 2023-02-03 | 杨开健 | Flame-retardant fabric finishing agent and fabric finishing method |
| CN118063643A (en) * | 2024-04-18 | 2024-05-24 | 康源领鲜科技有限公司 | Enteromorpha polysaccharide extraction process and application thereof in salmon feed |
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| KR101696076B1 (en) | 2017-01-13 |
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