KR20160145328A

KR20160145328A - Pharmaceutical composition for preventing or treating coccidiosis of chicken

Info

Publication number: KR20160145328A
Application number: KR1020150081751A
Authority: KR
Inventors: 유영국
Original assignee: 주식회사 케어사이드
Priority date: 2015-06-10
Filing date: 2015-06-10
Publication date: 2016-12-20

Abstract

The present invention relates to a pharmaceutical composition containing oregano oil and a Cucumis sativus extract for preventing or treating coccidiosis in chicken. The pharmaceutical composition further contains an emulsifier.

Description

[0001] PHARMACEUTICAL COMPOSITION FOR PREVENTING OR TREATING COCCIDIOSIS OF CHICKEN [0002]

The invention relates to a pharmaceutical composition for the treatment or prevention of coccidiosis in chicken.

The protozoa of genus Eimeria are an important cause of economic damage in the global poultry industry, about $ 3 billion a year (Dalloul and Lillehoj, 2006; Williams, 1999). In chickens infected with Eimeria spp., Weight loss due to decreased diarrhea, weakness, and feed efficiency is likely to occur, hemorrhage due to intestinal lesions is likely to occur, and infection with other pathogens such as bacteria or viruses (Morris and Gasser , 2006). The infection rate of domestic poultry farms by chicken bullpox has reached 5 ~ 70% (Du and Hu, 2004).

As a protozoa that induces bulbosis in chickens, seven species (E. acervulina, E. brunetti, E. maxima, E. mitis, E. necatrix, E. praecox, and E. tenella) Eimeria tenella, parasitic to the cecum, is the most virulent and has a high mortality, causing economic damage to poultry farms (Allen and Fetterer, 2002).

Although anticanc- aidic agents effectively inhibit chickenshopping, chaperones with drug resistance due to long-term use are likely to appear (Chapman, 1997; Usman et al., 2011) All were forbidden. In Korea, antibiotics are prohibited in 2012 and it is very urgent to develop anticoccidial substances among natural substances (Yang, 2006; Casewell et al., 2003).

The invention is intended to provide a pharmaceutical composition for treating or preventing coccidia in chicken using a natural substance as an active ingredient.

The present invention has been made in view of the above problems, and it is an object of the present invention to provide an apparatus and method for controlling the same.

The pharmaceutical composition for the treatment or prevention of coccidia in chicken according to the present invention includes oregano oil and a crude oil extract.

The pharmaceutical composition may further comprise an emulsifier. The emulsifier may be selected from the group consisting of Lecithin, Glycerin fatty acid ester, Glycerin fatty acid ester derivatives, Sucrose fatty acid fatty acid (Sugar ester), Sorbitan fatty acid ester acid ester, polyoxyethylene sorbitan fatty acid ester, polyglycerin fatty acid ester, glycerin, propylene glycol, and polysorbate.

The volume ratio of the oregano oil and the oil extract may be 1: 4 or more and 1: 5 or less.

The volume ratio of the oregano oil and the emulsifier may be 1: 4 or more to 1: 5 or less.

The bubbles of the oil extract and the emulsifier may be 1: 0.8 or more and 1: 1.2 or less. Preferably, the bubbles of the oil extract and the emulsifier may be 1: 1.

The details of other embodiments are included in the detailed description and drawings.

The present invention can provide a pharmaceutical composition for treating or preventing coccidiosis in chicken using a natural substance as an active ingredient.

The effects according to the invention are not limited by the contents exemplified above, and more various effects are included in the specification.

FIG. 1 is a graph showing the survival rate of the experimental group and the control group.
FIG. 2 is a graph showing a comparison of weight gain between the experimental group and the control group.
Figure 3 is a comparative graph of the number of feces in the feces of the experimental and control groups.
Figure 4 shows the results for the minimum inhibitory concentration for Salmonella typhimurium .

Advantages and features of the invention, and how to achieve them, will become apparent with reference to the following detailed examples.

The oregano oil may lower the activity of the chicken coccidium protozoa. The crude extract protects the damaged barrier from cocci chicken parasites and can not only stop the bleeding but also treat diarrhea.

Within this volume ratio, the Oregano oil can lower the activity of the chicken coccidium protozoa, which prevents the coccidiosis of the chicken from damaging the barrier, thereby preventing secondary infection by the coccidium chicken cockroach, treating diarrhea .

The bubbles of the oil extract and the emulsifier may be 1: 0.8 or more and 1: 1.2 or less. In order to improve the stability, the volume ratio of the oil extract to the emulsifier may preferably be 1: 1.

Experimental Example One: Anticoccid Resistant bacteria About oregano Oil-based Anticoccid Check the effect

Experiments were conducted according to the "Guidelines for the Management of Clinical Trial for Veterinary Drugs, etc." in Annex 2013-35 of the Agriculture, Forestry and Livestock Quarantine Division, by setting experimental groups and control groups as shown in Table 1 below. Specifically, survival rate, blood level, feed conversion ratio, relative growth rate, fecal incontinence (OPG) and intestinal tolerance test were performed.

Drug administration Insect infestation feed Target animal / number (horses) Control 1 Normal negative number × Non-antibiotic SPF chicken / 15 Experimental group Compound preparation ○ Non-antibiotic SPF chicken / 15 Control group 2 Normal negative number ○ Non-antibiotic SPF chicken / 15 Control group 3 Normal negative number ○ 1ppm Diclazuril supplemented feed SPF chicken / 15

Pest Infection: SPF Chicken Eimeria a tenella, E. acervulina mixed culture ^{8.8 × 10 4/300 ㎕ (} 1.3 × 10 4/300 ㎕ by E. tenella) were inoculated.

Compound preparation: 10% by volume of oregano oil, 40% by volume of a crude oil extract and 50% by volume of an emulsifier were used.

FIG. 1 is a graph showing the survival rate of the experimental group and the control group. As shown in FIG. 1, the survival rate was up to 14 days after infection. The survival rate of the experimental group was significantly higher than that of the control group (0.005 <p, log-rank test) It looked.

FIG. 2 is a graph showing a comparison of weight gain between the experimental group and the control group. 2, the average value of body weight gain from 7 to 14 days after infection was significantly higher in the experimental group (0.05 <p, t-test) than in the control group 2, and the feed efficiency (FCR) 2 and 3, respectively. It was confirmed that the combination preparation could affect the intake and the amount of body weight after infection.

* Feed efficiency = (feed intake / weight gain)

At the time of visual confirmation, the experimental group showed petechial hemorrhage in the duodenum but only a few hemorrhages were observed in the cecum, and the content was normal (lesion score 1) without thickening and the appearance was better than the control group 2 which was filled with blood and cheese- lesion score 4). The experimental group showed similar results to the control group 3.

In the case of hemodilution, the experimental group, the control group 2 and the control group 3 showed similar pattern, but the control group 2 had a longer duration and a severe degree of hemorrhage. Table 2 below shows the results of hemodilution from 4 to 8 days after infection. The severity of the stool was marked with a score from 0 to 4. Normal feces was 0, weak blood was 1, weak blood was 2, severe blood was 3, and very severe blood was 4.

4 days 5 days 6 days 7 days 8 days Control 1 0 0 0 0 0 Experimental group 2 3 One 0 0 Control group 2 3 4 2 One 0 Control group 3 2 3 One 0 0

Figure 3 is a comparative graph of the number of feces in the feces of the experimental and control groups. As shown in FIG. 3, the number of ovipositor feces in the feces was observed at 3 days, 7 days, 10 days, 14 days, and 16 days after the infection. As a result, Respectively.

Experimental Example 2: Length measurement of villi

The length of the villi by H & E staining was similar to that of the control group 1 and significantly different from that of the control group 2 and 3.

Duodenum Chairman Caecum Control 1 135.26 ± 8.62 88.41 + - 7.01 63.05 + - 4.36 Experimental group 121.53 + - 4.88 69.60 ± 3.23 65.06 + - 5.63 Control group 2 102.77 + - 8.93 72.573 + - 6.94 62.08 + - 14.18 Control group 3 42.43 + - 6.48 56.97 + - 6.83 56.73 ± 19.12

Experimental Example 3: oregano Comparison of Productivity and Economicality of Oil Combination Preparations

Control group and administration groups were set up as shown in Table 4 below, and the mortality rate, growth rate and the like according to the administration of the oregano oil combination preparation were confirmed. The feed was fed with no antibiotics.

Duration of administration (30 days) administration Target animal / number (horses) Control group Meitou × Local chicken / 50 Treatment group 1 Before Compound preparation Local chicken / 50 Group 2 Up to 2 weeks Compound preparation Local chicken / 50 Group 3 Up to 3 weeks Compound preparation Local chicken / 50 Group 4 Up to 4 weeks Compound preparation Local chicken / 50

There was a difference in body weight of approximately 360 g at the time of shipment (90 days old) in the treatment group 2 compared to the control group and the treatment groups 3 and 4. In other words, the administration group 2 was the most effective.

Experimental Example 4: oregano Salmonella of oil typhimurium The inhibitory effect of

Sample 1 was a mixture of 100% crude oil extract and the concentration of 100% crude oil extract was changed from 0.02% by volume to 1.00% by volume.

Sample 2 was a mixture of 100% oregano oil, and the concentration of 100% oregano oil was varied from 0.005% by volume to 0.030% by volume.

Sample 3 was a combination preparation containing 10 vol% oregano oil, 40 vol% of an oil-in-water extract and 50 vol% emulsifier, and the concentration of the combination preparation was varied from 0.05 vol% to 0.30 vol%.

Table 5 below shows the results of the minimum inhibitory concentrations of Salmonella typhimurium of Samples 1 to 3.

MIC Sample 1 1.00 vol% Sample 2 0.015% Sample 3 0.15 vol%

Referring to Table 5 and FIG. 4, it was confirmed that the combined preparation had an inhibitory effect of Salmonella typhimurium at 0.15% by volume.

The above-described experimental examples and the like are provided to aid understanding of the invention, and the technical idea of the invention is not limited to the above-described experimental examples and the like. Substitutions, additions, deletions, and the like of other elements within the scope of not hurting the technical idea of the invention will still be included in the present invention and constitute the contents of the invention.

Claims

Oregano oil; And
Oil extracts;
Or a pharmaceutically acceptable salt thereof.

The method according to claim 1,
A pharmaceutical composition for treating or preventing coccidia in chicken, which further comprises an emulsifier.

3. The method of claim 2,
The emulsifier may be selected from the group consisting of Lecithin, Glycerin fatty acid ester, Glycerin fatty acid ester derivatives, Sucrose fatty acid fatty acid (Sugar ester), Sorbitan fatty acid ester wherein the composition is at least one of polyoxyethylene sorbitan fatty acid esters, polyglycerin fatty acid esters, glycerin, propylene glycol, and polysorbate.

3. The method of claim 2,
Wherein the volume ratio of the oregano oil and the oil extract is 1: 4 or more to 1: 5 or less,
Wherein the volume ratio of the oregano oil and the emulsifier is 1: 4 or more to 1: 5 or less.

5. The method of claim 4,
Wherein the ratio of the crude oil extract to the emulsifier is 1: 0.8 or more to 1: 1.2 or less.

6. The method of claim 5,
A pharmaceutical composition for the treatment or prophylaxis of coccidia in chicken, wherein the ratio of the ratio of the crude oil extract to the emulsifier is 1: 1.