KR20160144229A - Lactobacillus plantarum KMM10 capable of fermenting at room temperature and composition for comprising the same - Google Patents

Abstract

The present invention relates to a novel Lactobacillus plantarum KMM10 isolated from Korean traditional fermented foods, and to a composition containing the same. According to the present invention, the Lactobacillus plantarum KMM10 exhibits excellent acid resistance and bile resistance while having antibacterial activities against harmful bacteria such as Helicobacter pylori or Streptococcus mutans, thereby being useful, as probiotics, for preventing cavity or intestinal and stomach disease for human and animal and also for strengthening immune functions. Furthermore, the Lactobacillus plantarum KMM10 can also be useful as a starter for fermentation owing to fermenting functions even at a room temperature.

Description

Lactobacillus plantarum KMM10 capable of fermentation at room temperature and a composition comprising the same,

The present invention relates to novel Lactobacillus plantarum KMM10 isolated from Korean traditional fermented foods and compositions comprising same.

Probiotics are collectively referred to as living microorganisms that have beneficial effects on the health of the host by improving the host microbial environment in the gastrointestinal tract of animals, including humans. Generally, probiotics include a range of beneficial bacteria and yeasts such as lactic acid bacteria and bifidobacteria. Lactic acid bacteria are widely found in nature and produce carbohydrates by anaerobic use of carbohydrates. The natural environment in which lactic acid bacteria are found is not only present in the intestines of humans and animals but also in various vegetables and fruits. It plays an important role in the fermentation process in yogurt, Korean kimchi and German fermented foods such as sauerkraut .

In order for lactobacillus to be effective as a probiotic, it must be ingested orally and adhered to the small intestine and maintained on the intestine surface, so that it should basically be excellent in acid resistance, bile acid resistance and adherent epithelial cells. Although various lactic acid bacteria have been reported so far, there is a growing demand for lactic acid bacteria suitable for room temperature fermentation.

The present invention relates to a novel Lactobacillus lactic acid bacterial strain having excellent antimicrobial activity against Helicobacter pylori and Streptococcus mutans strains and having an advantageous effect at room temperature fermentation as well as being excellent in acid resistance and bile resistance, ≪ / RTI >

Accordingly, the present inventors have made efforts to find lactic acid bacteria strains showing excellent effects as probiotics from traditional fermented foods. As a result, the present inventors have completed the present invention by isolating and identifying Lactobacillus plantarum KMM10, a novel lactobacillus lactic acid bacterium strain.

Lactobacillus plantarum KMM10 according to the present invention is a novel strain of Lactobacillus plantarum derived from traditional fermented food. The lactic acid bacteria strain showing excellent effect as probiotics from the conventional fermented food separated through the examples of the present invention has a nucleic acid sequence of SEQ ID NO: 1 as a result of 16S rDNA sequence analysis for identification and classification of microorganisms.

The microorganism of the present invention having the 16S rDNA sequence of SEQ ID NO: 1 was named Lactobacillus plantarum KMM10 and deposited on Apr. 13, 2015 (Accession No. KCTC 12794 BP ) in the microorganism conservation center. .

The Lactobacillus plantarum KMM10 of the present invention is a facultative anaerobe capable of growing in both aerobic and anaerobic conditions, is a Gram-positive bacterium, has no spore formation, has no mobility and takes the form of bacillus.

The lactobacillus plantarum KMM10 of the present invention is a probiotic, and has a general dressing effect and immunity enhancing effect of lactic acid bacteria.

In the present invention, 'probiotics' are understood to mean living microorganisms which improve the intestinal microbial environment of a host in the gastrointestinal tract of an animal including a human, thereby beneficially affecting the health of the host. Probiotics are live microorganisms with probiotic activity and can be beneficially beneficial to host intestinal microflora when fed in human or animal-dried cell form or in fermented product form in the form of single or complex strains.

In order to be used as probiotics, basically, it should have acid resistance and biliary properties. In the following examples, it was confirmed that the lactobacillus plantarum KMM10 of the present invention exhibited a high survival rate in artificial gastric juice of pH 2 and artificial bile juice of pH 8. In addition, it was confirmed that the lactobacillus plantarum KMM10 of the present invention exhibited high antimicrobial activity against Helicobacter pylori and Streptococcus mutans, which are harmful bacteria living in the human gastrointestinal tract or oral cavity.

Therefore, the Lactobacillus plantarum KMM10 according to the present invention can be used as a probiotic for various uses such as prevention of human or animal suits, gastrointestinal diseases, tooth decay, immunity enhancement and the like.

Thus, in one embodiment of the present invention, there is provided a probiotic composition comprising Lactobacillus plantarum KMM10.

The Lactobacillus plantarum KMM10 contained in the composition according to the present invention may exist as a living cell or a dead cell, and may be present in a form of dried or lyophilized. The forms and formulations of lactic acid bacteria suitable for inclusion in the various compositions are well known to those skilled in the art.

In one embodiment of the invention, there is provided a dressing composition comprising Lactobacillus plantarum KMM10. The composition according to the present invention can be used for prevention, treatment, and improvement of gastrointestinal diseases in animals including humans, and preferably the animals include cattle such as cows, horses, and pigs. Examples of the gastrointestinal diseases include infectious diarrhea caused by pathogenic microorganisms (Escherichia coli, Salmonella, Clostridium, etc.), gastrointestinal inflammation, inflammatory bowel disease, neurogenic enteritis syndrome , Intestinal microorganisms and growths, intestinal diarrhea, and the like.

 The composition of the present invention is preferably orally administered. The dosage may vary depending on the type of gastrointestinal disorder, the severity of the disease, age, sex, race, treatment or prevention purpose, but in general, from 10 million to 100 billion can be administered on an adult basis.

The present invention also provides a composition for inhibiting Helicobacter pylori comprising Lactobacillus plantarum KMM10. The present invention provides a composition for prevention and improvement of gastrointestinal diseases comprising Lactobacillus plantarum KMM10.

The present invention also provides a composition for inhibiting Streptococcus mutans bacteria comprising Lactobacillus plantarum KMM10. Further, the present invention provides a composition for preventing tooth decay comprising Lactobacillus plantarum KMM10.

The present invention also provides a composition for enhancing immunity comprising Lactobacillus plantarum KMM10. It is well known that Lactobacillus of the genus Lactobacillus has the effect of enhancing the effect of succulent effect.

Lactobacillus plantarum KMM10 according to the present invention may be contained in a pharmaceutical, health functional food, food, feed, or feed additive composition due to such advantageous action.

When the composition of the present invention is used as a pharmaceutical composition, the pharmaceutical composition of the present invention may be prepared by using pharmaceutically acceptable and physiologically acceptable adjuvants in addition to the above-mentioned active ingredients. Examples of the adjuvants include excipients, Release agents, sweeteners, binders, coating agents, swelling agents, lubricants, lubricants or flavors.

The pharmaceutical composition may be formulated into a pharmaceutical composition containing at least one pharmaceutically acceptable carrier in addition to the above-described active ingredients for administration.

For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Also, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic gums such as corn sweeteners, acacia, tracker candles or sodium oleate, sodium stearate, magnesium stearate, Sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum and the like. Acceptable pharmaceutical carriers for compositions that are formulated into a liquid solution include those suitable for sterilization and in vivo such as saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, One or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.

Further, it can be suitably formulated according to each disease or ingredient by using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field.

The present invention also provides a food composition comprising Lactobacillus plantarum KMM10 as an active ingredient. The food composition according to the present invention can be formulated in the same manner as the above pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, vitamin complexes, and health supplement foods.

The food composition of the present invention may contain components that are conventionally added during the manufacture of a food product, including, for example, proteins, carbohydrates, fats, nutrients, flavoring agents, and flavoring agents. Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings. For example, when the food composition of the present invention is prepared from a drink and a beverage, it may further include citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, and various plant extracts.

The composition according to the present invention can be used as a feed additive or feed.

When used as a feed additive, the composition may be 20 to 90% high concentrate or may be prepared in powder or granular form. The feed additive may be selected from the group consisting of organic acids such as citric acid, fumaric acid, adipic acid, lactic acid and malic acid, phosphates such as sodium phosphate, potassium phosphate, acid pyrophosphate, polyphosphate (polymerized phosphate), polyphenol, catechin, alpha-tocopherol, Extract, vitamin C, green tea extract, licorice extract, chitosan, tannic acid, phytic acid, and the like. When used as a feed, the composition may be formulated in conventional feed form and may contain conventional feed ingredients.

The feed additives and feeds may be selected from the group consisting of cereals, such as ground or crushed wheat, oats, barley, corn and rice; Feeds based on vegetable protein such as rapeseed, soybeans and sunflower; Animal protein feeds such as blood, meat, bone meal and fish meal; A sugar or a milk product, for example, a dry component comprising various powdered milk and whey powder, and may further include nutritional supplements, digestion and absorption enhancers, growth promoters, and the like.

The feed additive may be administered to animals singly or in combination with other feed additives in edible carriers. The feed additives can also be administered to the animal either as a top dressing or they can be mixed directly with the animal feed or in a separate oral form separate from the feed. When the feed additive is administered separately from an animal feed, it can be prepared in an immediate release or sustained release formulation, in combination with a pharmaceutically acceptable edible carrier as is well known in the art. Such edible carriers may be solid or liquid, such as corn starch, lactose, sucrose, soy flakes, peanut oil, olive oil, sesame oil and propylene glycol. When a solid carrier is used, the feed additive can be a tablet, capsule, powder, troche or emulsion or top-dressing in finely divided form. When a liquid carrier is used, the feed additive can be a gelatin soft capsule, or a syrup or suspension, emulsion, or solution formulation.

The feed may comprise any protein-containing organic fructose commonly used to meet an animal's dietary needs. These protein-containing flours usually consist mainly of corn, soy flour, or corn / soy flour mix.

The feed additives and feeds may also contain adjuvants such as preservatives, stabilizers, wetting or emulsifying agents, solution promoters and the like. The feed additive may be used by adding to the animal's feed by pouring, spraying or mixing.

The feed or feed additive of the present invention can be applied to a number of animal diets including mammals, poultry, and fish.

The above-mentioned mammals can be used for pets (for example, dogs and cats) as well as pigs, cows, sheep, goats, laboratory animals and laboratory animals as well as poultry such as chicken, turkey, duck, goose, And quail, and the fish can be used as trout, but the present invention is not limited thereto.

 Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described in detail below. The present invention may, however, be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. Is provided to fully convey the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims.

Lactobacillus plantarum KMM10 according to the present invention is not only excellent in acid resistance and bile resistance but also exhibits antimicrobial activity against harmful bacteria such as Helicobacter pylori or Streptococcus mutans. Therefore, lactobacillus plantarum KMM10 can be used as a probiotic, Or prevention of tooth decay, immunity enhancement, and the like. Furthermore, since fermentation can be performed at room temperature, it can be usefully used as a starter for fermentation.

1 shows Lactobacillus plantarum KMM10 observed through an electron microscope of the present invention.
Fig. 2 shows the acid resistance test results of Lactobacillus plantarum KMM10 according to the present invention.
FIG. 3 shows the results of the bile test of lactobacillus plantarum KMM10 according to the present invention.
FIG. 4 shows the results of measurement of viable counts of Streptococcus mutans strains mixed with Lactobacillus plantarum KMM10 of the present invention.

Hereinafter, the present invention will be described in detail with reference to examples. The following examples illustrate the invention and are not intended to limit the scope of the invention.

[ Example ]

Example  1: Isolation and selection of lactobacillus that can be incubated at room temperature

The strains cultured at room temperature (25 ℃) in Korean traditional fermented foods were selected. The whole contents of traditional fermented foods such as kimchi, soybean paste, soy sauce, etc. were crushed and then filtered with sterile gauze. The filtrate was diluted stepwise with sterilized water, and then 100 μL of the solution was firstly plated on a MRS (Lactobacilli MRS agar, Difco) plate medium supplemented with 2% calcium carbonate (CaCO3) and cultured at 30 ° C. The culture broths which showed a transparent ring during culture and were separated were isolated and plated on MRS plate medium containing 0.006% BCP (Bromocresol Purple, Sigma, St. Louis, Mo., USA) Respectively. Among them, the purple culture medium of BCP was changed into yellow, and the culture was applied to a new BCP medium. The culture was repeated three times until pure lactic acid bacteria were obtained.

Lactic acid bacteria were isolated and identified by confirming the mycological characteristics of isolated strains. Lactic acid bacteria were inoculated on MRS plate medium and MRS liquid medium and incubated at 25 ° C for 12 hours.

The acidic and biliary properties of the selected lactic acid bacteria were measured as in the following examples. In the case of strains having excellent acid resistance and biliary excretion, Gram stainability is positive, cell type is negative for bacillus, catalase is negative, mobility is negative, apoploid is negative, and lactic acid production ability is positive. Thus, strains belonging to the genus Lactobacillus , And the lactic acid bacteria belonging to Lactobacillus plantarum were finally selected. Figure 1 shows Lactobacillus plantarum KMM10 observed through an electron microscope.

Example  2: Lactobacillus Planta Rum KMM10 The identification of

As a result of 16S rDNA sequencing analysis for identification of microorganisms, the strains isolated through the examples of the present invention have the nucleotide sequence of SEQ ID NO: 1.

As a result of the similarity analysis of the 16S rDNA sequence of the selected lactic acid bacteria, the basic local alignment search tool (BLAST) of the National Center for Biotechnology Information (NCBI, www.ncbi.nlm.nih.gov ) It belongs to lanta rum.

Thus, the microorganism of the present invention was named Lactobacillus plantarum KMM10 and deposited at the Korean Microorganism Conservation Center on Apr. 13, 2015 (accession number KCTC 12794BP).

≪ SEQ ID NO: 1 > 16S rDNA of Lactobacillus plantarum KMM10

TGCTCCATCAGACTTTCGTCCATTGTGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTGTCTCAGTCCCAATGTGGCCGATTACCCTCTCAGGTCGGCTACGTATCATTGCCATGGTGAGCCGTTACCCCACCATCTAGCTAATACGCCGCGGGACCATCCAAAAGTGATAGCCGAAGCCATCTTTCAAACTCGGACCATGCGGTCCAAGTTGTTATGCGGTATTAGCATCTGTTTCCAGGTGTTATCCCCCGCTTCTGGGCAGGTTTCCCACGTGTTACTCACCAGTTCGCCACTCACTCAAATGTAAATCATGATGCAAGCACCAATCAATACCAGAGTTCGTTCGACTAACATTAT

Example  3: Lactobacillus Planta Rum KMM10 of Acid resistance  And My bile  analysis

The resistance of lactic acid bacteria to artificial gastric juice and artificial bile juice was investigated by Huang and Adams (In vitro assessment of the upper gastrointestinal tolerance of probiotic dairy propionibacteria. International Journal of Food Microbiology 91: 253-260, 2004), and proceeded using artificial gastric juices and artificial bile solutions.

Artificial gastric juice consisted of phosphate buffer solution adjusted to pH 2, 12 mM NaCl, 7 mM KCl, 45 mM NaHCO3 and 1 mg / ml pepsin (Sigma-Aldrich, USA).

The artificial bile solution was adjusted to pH 8 with NaOH after adding 1 mg / ml pancreatin (Sigma-Aldrich, USA) and 0.5% bovine bile solution (Sigma-Aldrich, USA) to phosphate buffer solution.

The acid tolerance test in artificial gastric juice was carried out by adding 1 g of Lactobacillus plantarum KMM10 isolated and identified in Examples 1 and 2 to artificial gastric juice, incubating at 37 ° C for 30 minutes, 60 minutes and 120 minutes Thereafter, 1 ml of the culture broth was collected. The cultured medium was diluted with phosphate buffered saline (pH 7), cultured on standard MRS agar medium by standard agar plate culture, viable counts were measured and expressed as cfu.

In the artificial bile test, the bacterium was collected from the artificial bile solution after 60 minutes, 120 minutes and 240 minutes after inoculation of 1 g of the lactic acid bacterium. The viability of the culture was measured by the same method as the acid resistance evaluation method.

The results are shown in Fig. 2 and Fig. 2 is a graph showing the acid resistance test results for Lactobacillus plantarum KMM10. FIG. 3 is a graph showing the results of the bile test for lactobacillus plantarum KMM10. FIG.

According to the results shown in FIG. 2 and FIG. 3, Lactobacillus plantarum KMM10 showed about 59.8% of the bacterial count before incubation 120 minutes after the incubation, It was confirmed that about 59.8% of them had acid resistance, and about 71.1% of them had biliary properties. This indicates that the novel strain according to the present invention can reach the intestine without being affected by gastric juice in the body and survive in the intestines without being affected by bile.

Example  4: Lactobacillus Planta Rum KMM10 Antimicrobial activity of

(a) Helicobacter Pyorrhoid ( Helicobacter pylori ) Infection Test

To determine the antimicrobial activity of Lactobacillus plantarum KMM10, Helicobacter pylori (ATCC 43504) strain was used.

As a control, Helicobacter pylori was inoculated into a 5% (v / v) fetal bovine serum (FBS; Gibco BRL. USA), 0.2% (w / v) 2,6- (10% CO ₂ , Anoxomat system, MART co., Netherland, NJ) was inoculated on a brucella broth medium (Difco) containing β-cyclodextrin and antibiotics (ceftarodine, vancomycin, trimethoprim and amphotericin B) ) At 37 < 0 > C for 48 hours. The cultured Helicobacter pylori culture broth was centrifuged at 7,000 rpm for 10 minutes at 4 ° C, and the cell precipitate was recovered and washed twice with phosphate buffer solution of pH 7. 1x10 ⁵ cfu / ml of Helicobacter pylori cell suspension was inoculated into 10 ml of brucella broth without antibiotics. Lactobacilli were added as a sample and incubated at 37 ° C under aerobic conditions (10% CO ₂ , Anoxomat system, MART co., Netherland) For 48 hours, and the number of Helicobacter pylori was determined by standard agar plate culture method. Helicobacter pylori and Lactobacillus plantarum KMM10 were mixed and cultured to test the antimicrobial activity of the novel strains. Helicobacter pylori and Lactobacillus plantarum KMM10 were added to 5% and 10%, respectively, and cultured in the same manner as the above-mentioned control to determine the number of Helicobacter pylori bacteria. The results of observing changes in the number of Helicobacter pylori bacteria at 12 hours and 24 hours after culturing in brucella broth are shown in Table 1 below.

Lactic acid Viable cell counts (Log CFU / ml) Control 5% 10% 0h 12h 24h 0h 12h 24h 0h 12h 24h Lactobacillus plantarum
KMM10 5.48 ±
0.42 6.25 ±
0.63 8.13 ±
0.15 5.34 ±
0.30 5.00 ±
0.47 * 6.19 ±
0.36 * 5.78 ±
0.50 4.82 ±
0.27 * 4.14 ±
0.41 * Data are means ± SD from triplicate determinations.
Significantly different from the control (p <0.05).

According to the results shown in Table 1, the number of Helicobacter pylori bacteria in the brucella broth cultured for 12 hours without addition of Lactobacillus plantarum KMM10 was about 6.25 ± 0.63 log cfu / ml, and after 24 hours, it was 8.13 ± 0.15 log cfu / ml. After addition of 5% Lactobacillus plantarum KMM10 for 24 hours, it was 6.19 ± 0.36 log cfu / ml, which was about 2 log cycle lower than that of the control.

On the other hand, when 10% of Lactobacillus plantarum KMM10 was added, it was found that the antibacterial effect was effective against Helicobacter pylori. When 10% Lactobacillus plantarum KMM10 and Helicobacter pylori were mixed, the number of Helicobacter pylori bacteria after 24 hours was 4.14 ± 0.41 log cfu / ml, which was about 4 log cycle lower than that of the control. This indicates that the addition of 10% or more of Lactobacillus plantarum KMM10 results in a higher antibacterial effect on Helicobacter pylori.

(b) Streptococcus Mutans ( Streptococcus mutans ) Infection Test

Streptococcus mutans strain was used as another example to examine the antimicrobial activity of Lactobacillus plantarum KMM10. 2 ul of Streptococcus mutans was inoculated into 20 ml of TSB (Tryptic soy broth) and cultured at 37 ° C. Lactobacillus plantarum KMM10 was cultured in TSB as a general medium. After confirming its growth, the cells were inoculated into TSB medium at a concentration of 5 × 10 ⁶ cfu / ml, and cultured for 24 hours at 37 ° C. The degree of inhibition of growth was examined. As shown in FIG. 4, as the concentration of Lactobacillus plantarum KMM10 was increased, the number of viable cells of Streptococcus mutans was decreased.

Example  5: Lactobacillus Planta Rum KMM10 Of lactic acid bacteria powder

In order to prepare lactobacillus powder of Lactobacillus plantarum KMM10, the above lactic acid bacteria were inoculated in an MRS broth medium (Becton & Dickinson) at 1 × 10 ⁷ cfu / ml and cultured at 37 ° C. for 12 hours to 18 hours, followed by centrifugation And the cells were recovered. The recovered cells were washed with 0.85% NaCl, centrifuged again, washed with 10% defatted powdered milk and 1% sodium glutamate solution, and the cells were suspended. The suspension was lyophilized by a conventional method to powder the lactic acid bacteria.

Example  6: Skim milk powder  Fermentation test in medium

The 10% (w / w) reduced skim milk medium obtained by dissolving the skimmed milk powder (Difco) in water was sterilized at 95 ° C for 30 minutes, and the starter of each strain was added to 1% (v / v) and then cultured at 25 ° C, 30 ° C and 37 ° C for 12 hours. The obtained culture solution was quenched to determine the degree of solidification, the pH and the number of lactic acid bacteria contained therein. The measurement of the number of lactic acid bacteria was carried out on standard agar medium supplemented with commercial BCP.

The measurement results of the above experiment are shown in Table 2 below.

25 ℃ 12 hours 30 ℃ 12 hours 37 ℃ 12 hours Number of bacteria pH Appearance Number of bacteria pH Appearance Number of bacteria pH Appearance Lactobacillus plantarum KMM10 6.5 × 10 ¹⁰ 4.65 yogurt
coagulation 7.2 × 10 ¹⁰ 4.48 yogurt
coagulation 4.5 × 10 ¹⁰ 4.59 yogurt
coagulation

According to the results shown in Table 2, when the strain of Lactobacillus plantarum KMM10 was used, the pH was lowered to 4.4 to 4.6 at any temperature, and the medium solidified. In addition, the number of lactic acid bacteria contained therein was increased to about 1 × ^{10 9} cfu / g, and fermentability was confirmed.

Therefore, it can be seen that Lactobacillus plantarum KMM10 used in the present invention has high availability as an easy fermentation starter for culturing at room temperature (25 DEG C).

Korea Microorganism Conservation Center (Domestic) KCTC12794BP 20150413

<110> GANGNEUNG-WONJU NATIONAL UNIVERSITY INDUSTRY ACADEMY COOPERATION GROUP <120> Lactobacillus plantarum KMM10 capable of fermenting at room          temperature and composition for the same <130> P15U12C0622 <160> 1 <170> KoPatentin 3.0 <210> 1 <211> 1394 <212> DNA <213> Lactobacillus plantarum <400> 1 aactctcatg gtgtgacggg cggtgtgtac aaggcccggg aacgtattca ccgcggcatg 60 ctgatccgcg attactagcg attccgactt catgtaggcg agttgcagcc tacaatccga 120 actgagaatg gctttaagag attagcttac tctcgcgagt tcgcaactcg ttgtaccatc 180 cattgtagca cgtgtgtagc ccaggtcata aggggcatga tgatttgacg tcatccccac 240 cttcctccgg tttgtcaccg gcagtctcac cagagtgccc aacttaatgc tggcaactga 300 taataagggt tgcgctcgtt gcgggactta acccaacatc tcacgacacg agctgacgac 360 aaccatgcac cacctgtatc catgtccccg aagggaacgt ctaatctctt agatttgcat 420 agtatgtcaa gacctggtaa ggttcttcgc gtagcttcga attaaaccac atgctccacc 480 gcttgtgcgg gcccccgtca attcctttga gtttcagcct tgcggccgta ctccccaggc 540 ggaatgctta atgcgttagc tgcagcactg aagggcggaa accctccaac acttagcatt 600 catcgtttac ggtatggact accagggtat ctaatcctgt ttgctaccca tactttcgag 660 cctcagcgtc agttacagac cagacagccg ccttcgccac tggtgttctt ccatatatct 720 acgcatttca ccgctacaca tggagttcca ctgtcctctt ctgcactcaa gtttcccagt 780 ttccgatgca cttcttcggt tgagccgaag gctttcacat cagacttaaa aaaccgcctg 840 cgctcgcttt acgcccaata aatccggaca acgcttgcca cctacgtatt accgcggctg 900 ctggcacgta gttagccgtg gctttctggt taaataccgt caatacctga acagttactc 960 tcagatatgt tcttctttaa caacagagtt ttacgagccg aaacccttct tcactcacgc 1020 ggcgttgctc catcagactt tcgtccattg tggaagattc cctactgctg cctcccgtag 1080 gagtttgggc cgtgtctcag tcccaatgtg gccgattacc ctctcaggtc ggctacgtat 1140 cattgccatg gtgagccgtt accccaccat ctagctaata cgccgcggga ccatccaaaa 1200 gtgatagccg aagccatctt tcaaactcgg accatgcggt ccaagttgtt atgcggtatt 1260 agcatctgtt tccaggtgtt atcccccgct tctgggcagg tttcccacgt gttactcacc 1320 agttcgccac tcactcaaat gtaaatcatg atgcaagcac caatcaatac cagagttcgt 1380 tcgactaaca ttat 1394

Claims (6)

Lactobacillus plantarum KMM10 with accession number KCTC 12794BP. Lactobacillus plantarum KMM10. A composition for suppressing Helicobacter pylori comprising Lactobacillus plantarum KMM10. A composition for inhibiting Streptococcus mutans strain comprising Lactobacillus plantarum KMM10. A feed or feed additive composition comprising Lactobacillus plantarum KMM10. Lactobacillus plantarum Lactobacillus starter for fermentation at room temperature containing KMM10.

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