KR20160132627A

KR20160132627A - Method for Detecting Target Material Using Surface Capacitive Touchscreen

Info

Publication number: KR20160132627A
Application number: KR1020150065412A
Authority: KR
Inventors: 박현규; 원병연; 안준기
Original assignee: 한국과학기술원
Priority date: 2015-05-11
Filing date: 2015-05-11
Publication date: 2016-11-21

Abstract

The present invention relates to a method of detecting a target material by using a surface-capacitance-type touch screen that receives a contact of a conductor as an input signal and outputs the input signal to a display screen. More specifically, the characteristics of the target substance whose electrical conductivity changes depending on whether or not it is bonded and the position of the contact signal appearing between the two points when the two points of the surface capacitive touch screen are contacted at the same time, And the presence or absence of the target substance in the sample and its concentration are detected by using the characteristic that the electric conductivity is shifted toward the higher point when the conductivity is different.
The method of detecting a target substance according to the present invention uses a touch screen of a surface-capacitance type which can be mass-produced and is inexpensive. Thus, It is a technology that can be easily applied to the development of a small target material detection device which is inexpensive, simple to use, and small in comparison with the conventional method which can be performed only in laboratories having skilled labor and facilities requiring time.

Description

TECHNICAL FIELD [0001] The present invention relates to a method of detecting a target material using a surface capacitive touch screen,

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for detecting a target material by using a surface-capacitance type touch screen that receives a contact of a conductor as an input signal and outputs the input signal to a display screen. More specifically, To a method of detecting a target substance by detecting a change in capacitance of a surface of a touch panel caused by a combination of the target substance and a probe.

A touch screen refers to a device designed to detect a character or specific position of a finger on a display screen (screen) instead of an input device such as a keyboard or a mouse, .

The touch screen is classified into a resistive type, a capacitive type, a SAW (surface acoustic wave) type, and an infrared (IR) type depending on the implementation of the touch panel. It is widely used in various mini terminal devices such as smart phone or tablet PC as capacitive type. The capacitance type touch screen is again divided into a surface capacitive type and a projected capacitive type. The present invention relates to a method of detecting a target material using a surface capacitance type touch screen . The surface capacitive touch screen will be briefly described as follows. The touch controller, which controls the operation of the touch screen system, applies a constant voltage to the touch panel on which the finger contacts, thereby forming an electric field on the surface of the touch panel. At this time, only the voltage is applied to the surface of the touch panel, and the current does not flow. When the panel is brought into contact with a conductor (human body, mainly a finger) or the like, current flows through the finger from the contact portion of the panel due to the electrostatic capacity of the human body, and resistance is generated accordingly. Since the current and resistance values are correlated with the current flowing distance, the current value and the resistance value caused by the contact of the finger from the electrode located at the four corners of the rectangular touch panel are measured, The touch controller calculates the distance at which the contact occurs, and thereby determines the contact position. Thus, the surface capacitive screen can detect only one touch position at a time. However, if two points are touched by a finger at the same time, the middle point between the two touched points is recognized as a contact signal because the electric conductivities of the two fingers are the same.

On the other hand, the field of in vitro diagnostics for analyzing biomolecules related to various diseases is a field for early detection of various diseases, a field for investigating the state of health or progress of diseases, and is a field for screening diseases, preventing diseases, , Personal health status tests, genetic tests, and non-medical fields, which are widely used in veterinary medicine, environmental management and food management. In particular, recently, there has been a national crisis due to the emergence of highly contagious diseases such as strains of influenza virus and foot-and-mouth disease, and there is still a high risk of occurrence, and the demand for improving the quality of life of the public is increasing. And the need for periodic health status tests and their importance have been highlighted. Accordingly, the analysis technology of biomolecules related to various diseases / diseases is very important economically and technically, and has been actively studied worldwide because it has a large ripple effect on the industry.

Currently, a representative method used for the examination and confirmation of various infectious diseases and genetic diseases is a method of detecting a pathogen gene, and a real-time PCR is currently being used as the most powerful method for genetic testing. However, because these analytical methods are based on fluorescent signals, they require bulky, expensive analytical instruments, or require skilled expertise or long analysis time. Therefore, most of the genetic diagnoses currently available are only available through referrals to universities / general hospitals or professional diagnostic institutions equipped with specialized facilities and personnel, and it takes a lot of time and money to collect samples and report results. In order to overcome these limitations, point-of-care testing (POCT), which can be used in local small hospitals and public health centers or at home, should be implemented. For this purpose, inexpensive and compact analysis Device development is required.

In Korean Patent No. 10-1193607, the present inventors have fixed a probe on a touch panel of a touch screen of a multi-contact capacitive touch screen, measured electrostatic capacities according to the combination of the injected biomolecules and the probe, And a method for detecting the same.

However, since the probe corresponding to the substance to be detected must be immobilized on the touch panel, the method has a disadvantage in that it takes a lot of time and effort to manufacture, and it can detect only a predetermined substance.

Therefore, the present inventors have made intensive efforts to develop a target substance detection method capable of on-site diagnosis in place of the existing high-cost gene diagnosis apparatus. As a result, they have found that a target substance whose electrical conductivity changes due to binding is contacted with a surface- It is confirmed that the target substance can be detected and quantified when the capacitance of the touch panel is directly or indirectly measured.

An object of the present invention is to provide a method of detecting a target substance by using a cheap and simple surface capacitance touch screen as a detection platform for developing a simple and efficient gene field diagnostic system.

In order to achieve the above object, the present invention provides a method of manufacturing a touch screen, comprising the steps of: (a) applying a probe complementary to a reference solution and a target material to two points on a panel of a surface- (b) hybridizing the sample containing the target substance by applying the same amount to each of the two sites; (c) an auxiliary panel coated with a transparent electrode coated with a conductive wire or coated with a transparent electrode on both sides, is laminated on the touch panel of the above (a) so that the surface coated with the transparent electrode contacts the touch panel Stacking; (d) contacting the touch conductor with a conductor connected to the transparent electrode or a transparent electrode on the opposite side in contact with the touch panel to cause a current to flow on the surface of the touch panel; And (e) detecting a target material by measuring a position of one touch signal using a touch controller. The present invention also provides a method of detecting a target material using a touch screen of a surface capacitance type.

(A) applying a probe complementary to a reference solution and a target material to two points on a touch panel of a touch screen of a surface capacitance type; (b) hybridizing the sample containing the target substance by applying the same amount to each of the two sites; (c) an auxiliary panel coated with a transparent electrode coated with a conductive wire or coated with a transparent electrode on both sides, is laminated on the touch panel of the above (a) so that the surface coated with the transparent electrode contacts the touch panel Stacking; (d) contacting the touch conductor with a conductor connected to the transparent electrode or a transparent electrode on the opposite side in contact with the touch panel to cause a current to flow on the surface of the touch panel; And (e) a step of detecting a target material by measuring a current with respect to one contact signal position and a contact signal position with a touch controller further comprising an analog signal output element for measuring and outputting a current of the touch panel A method of detecting a target substance using a capacitive touch screen is provided.

The present invention also provides a method of manufacturing a touch panel, comprising the steps of: (a) stacking an analytical frame including a sample inlet, a sample transfer channel, a sample contact position, and a tablet film on a touch panel of a touch screen of a surface capacitance type, (b) an auxiliary panel coated with a transparent electrode or a transparent electrode coated on both sides to which leads are connected, is laminated on the analysis frame of (a), and a surface coated with a transparent electrode is laminated on the analysis frame Laminating them so as to contact; (c) injecting a target substance and a probe to be detected into the sample injection port; (d) contacting the touch conductor with a conductor connected to the transparent electrode or a transparent electrode on the opposite side in contact with the touch panel to cause a current to flow on the surface of the touch panel; And (e) a step of detecting a target material by measuring a current with respect to one contact signal position and a contact signal position with a touch controller further comprising an analog signal output element for measuring and outputting a current of the touch panel A method of detecting a target substance using a capacitive touch screen is provided.

The present invention also relates to a capacitive touch screen in which a sample containing a target material is dispensed; And an auxiliary panel coated with a transparent electrode on the both sides thereof coated with a transparent electrode to which a conductive wire to which a touch conductor is to be connected is connected.

The method of detecting a target nucleic acid according to the present invention uses a surface-capacitance touch screen capable of being mass-produced and cost-effective since the present technology is completed, and thus it is necessary to provide a bulky and expensive fluorescent analyzer , Or a detection system that is inexpensive and has a short analysis time as compared with a conventional nucleic acid detection method that can be performed only in a laboratory equipped with a skilled workforce and facilities, Lt; / RTI > Specifically, since it is easy to implement a small field diagnostic apparatus equipped with a surface capacitance type touch screen, anyone can easily diagnose a disease infection or the like.

FIG. 1 is a graphical representation of the flow of current when two touch points of a surface capacitive touch screen are simultaneously contacted through an electrically conductive medium solution, and thus the touch signals appearing on the display.
2 is a schematic diagram illustrating a method of detecting a target nucleic acid using a surface capacitive touch screen system.
3 is a graphical illustration of an example of a contact signal that appears when two fixed positions A and B of a touch panel of a surface capacitive touch screen are simultaneously contacted. Is expressed by y.
4 is a graph showing a change in position (x / y) of a contact signal according to the electrical conductivity ratios of points A and B when two points A and B of the touch panel are simultaneously contacted through mediators of different electrical conductivities.
FIG. 5 shows the result of target nucleic acid (target) and probe nucleic acid (amplification) through asymmetric PCR through gel electrophoresis, wherein a represents a double stranded result and b represents a single stranded result.
6 (a) shows the position of the contact signal when the probe solution is contacted with the reference solution through the probe solution at the same time, (b) shows the contact signal when the probe solution and the probe nucleic acid solution contact each other simultaneously (C) shows the position of the contact signal when the probe is contacted simultaneously through the single-stranded probe solution and the probe-target nucleic acid hybridization solution.
FIG. 7 is a graph showing a target nucleic acid detection result. A dotted line is a graph that theoretically predicts a change in the position of a signal depending on the amount of a target nucleic acid applied, where a indicates a hybridization efficiency of 0% and b indicates a hybridization efficiency of 100% , And the plots indicated by the circles represent the results obtained by applying the actual detection sample. The empty circles represent the case where nonspecific nucleic acids are added, and the filled circles represent the cases where the target nucleic acid is added.
8 is a schematic diagram of a touch controller capable of outputting a contact signal position and a current to which an analog signal output element for directly outputting a current of the touch panel is added.
Figure 9 shows a surface capacitive touch screen system including a purification membrane capable of separating certain biomolecules.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is well known and commonly used in the art.

The surface capacitive touchscreen recognizes the touch points of the touch panel as touch signals at the same time, instead of recognizing the respective touches when touching two points of the touch panel at the same time with the finger. do. This is because the electrical conductivities of the two fingers are the same. If the two fingers touch the fingers and the fingers with thin gloves, the electrical signal on the fingers is high. Appear at a location closer to the point of contact. In addition, since the surface capacitance type touch screen does not contact directly with a finger but a current flows even if it contacts with an electrically conductive substance, it is recognized as an input signal, A signal appears (Fig. 1). Even in this case, since the magnitude of the current generated by the contact is proportional to the electrical conductivity of the mediator, when the two points are contacted in the same manner through the electrolytes having different electrical conductivities, The contact signal is moved to the position where it is located.

According to one aspect of the present invention, there is provided a method of manufacturing a touch screen, comprising the steps of: (a) applying a probe complementary to a reference solution and a target material to two points on a touch panel of a touch screen of a surface capacitance type; (b) hybridizing the sample containing the target substance by applying the same amount to each of the two sites; (c) an auxiliary panel coated with a transparent electrode coated with a conductive wire or coated with a transparent electrode on both sides, is laminated on the touch panel of the above (a) so that the surface coated with the transparent electrode contacts the touch panel Stacking; (d) contacting the touch conductor with a conductor connected to the transparent electrode or a transparent electrode on the opposite side in contact with the touch panel to cause a current to flow on the surface of the touch panel; And (e) detecting a target material by measuring a position of one touch signal using a touch controller. 2. Description of the Related Art

According to another aspect of the present invention, there is provided a method of manufacturing a touch sensor, comprising the steps of: (a) applying a probe complementary to a reference solution and a target material to two points on a touch panel of a touch screen of a surface capacitance type; (b) hybridizing the sample containing the target substance by applying the same amount to each of the two sites; (c) an auxiliary panel coated with a transparent electrode coated with a conductive wire or coated with a transparent electrode on both sides, is laminated on the touch panel of the above (a) so that the surface coated with the transparent electrode contacts the touch panel Stacking; (d) contacting the touch conductor with a conductor connected to the transparent electrode or a transparent electrode on the opposite side in contact with the touch panel to cause a current to flow on the surface of the touch panel; And (e) a step of detecting a target material by measuring a current with respect to one contact signal position and a contact signal position with a touch controller further comprising an analog signal output element for measuring and outputting a current of the touch panel And more particularly, to a method of detecting a target substance using a capacitive touch screen.

The term " application " in the present invention refers to all the actions of injecting a sample, a reference solution, a probe, etc. into a touch panel or an analysis frame, and may include dotting, dropping, spilling and the like.

In the present invention, the reference solution may be an electrolyte solution having a known concentration or a buffer solution having a composition that facilitates nucleic acid hybridization, and the target substance may have a change in electric conductivity of the touch panel The probe may be selected from the group consisting of a nucleic acid, a protein, an inorganic ion in a living body, and a mixture thereof. The probe may be combined with a target material to form a touch panel And a nucleic acid, a protein, and a mixture thereof capable of changing electrical conductivity.

In the present invention, the sub-panel may be selected from the group consisting of glass, acrylic, and plastic. The transparent electrode may be formed of indium tin oxide (ITO), zinc oxide (ZnO), indium- (IZO), gallium-zinc-oxide (GZO), aluminum-zinc-oxide (AZO) and carbon nanotubes (CNT) or graphene have.

In the present invention, the touch conductor may be selected from the group consisting of a finger, a stylus pen applicable to an electrostatic capacity type, and a touch glove applicable to an electrostatic capacity type, and the step (e) (L) of the distance between the position of the contact signal on the touch panel measured on the touch panel and the position of the reference solution applied on the touch panel, and the distance between the position of the reference solution and the position of the probe, L) is substituted into the equation prepared using the reference sample containing the target substance to be detected, and the ratio of the electric conductivity is calculated to determine whether the probe hybridizes with the target substance.

In the conventional capacitive touch screen, in order to recognize a change in capacitance as a touch signal, the controller determines a change in capacitance by a predetermined amount based on a change in capacitance, determines an effective input signal, Only the digital signal of However, since the current varying on the surface of the touch panel differs depending on whether or not the probe and the target material used for the touch are coupled, an analog signal output element capable of outputting a current value to the controller is added to directly output the current of the touch panel , The presence and concentration of the target substance can be measured. A schematic diagram of a touch controller to which an analog signal output element capable of outputting a current value is added is shown in Fig.

On the other hand, since the nucleic acid acts as an electrolyte in the state of being dissolved in the solution, the electrical conductivity of the solution varies depending on the concentration of the nucleic acid dissolved in the solution. Also, the electrical conductivities of single-stranded nucleic acids and double-stranded nucleic acids are also different. Thus, as described above, nucleic acid solutions can be applied as contact media for surface capacitive touch screens. As shown in FIG. 2, a solution containing a reference solution and a probe having a sequence specific to a target nucleic acid is applied to two fixed points (A and B) on the surface of a touch screen of a surface capacitance type, When the same amount is applied to both the A and B sites, when the target nucleic acid is present in the detection sample, the probe and the target nucleic acid are hybridized to each other at the site B to form a double strand nucleic acid, and a single strand target nucleic acid is present at the site A , Hybridization of the target nucleic acid and its amount can be calculated through a relational expression indicating the relationship between the amount of nucleic acid and the hybridization efficiency and the position of the contact signal based on the position of the contact signal appearing on the display when the two points are contacted at the same time.

That is, in one embodiment of the present invention, the change of the position and the electrical conductivity of the contact signal was measured, and it was confirmed that the ratio of the electrical conductivity was inversely proportional to the position of the connection signal (FIG. 4).

First, two points (A and B) where a medium nucleic acid solution is to be positioned are determined on the touch panel of a surface capacitance touch screen (FIG. 3), and the positions of contact signals appearing between two points when A and B are simultaneously contacted are A The distance between points is defined as x , and the distance between points A and B is defined as y . In the present invention, the x and y values are pixels representing the resolution on the display. The distance between A and B is fixed so that the y value is 850 pixels, and the position of the contact signal is represented by the symbol L , And L = x / y .

Since the electrical conductivity of the nucleic acid solution is proportional to the concentration of the nucleic acid, the concentration ratio of the two nucleic acid solutions can be represented by the electrical conductivity ratio of the nucleic acid solution. Two nucleic acid solutions of known concentration were added to positions A and B, respectively, and the position L of the signal when two points were contacted at the same time was measured. As a result of repeating the experiment using various concentrations of the nucleic acid solution, the following relation was derived between the position ( L ) of the contact signal and the ratio of the electrical conductivity ( C _A / C _B ) of the mediating solution (FIG.

(Equation 1) L = x / y = 1.42-0.29 ( C _A / C _B ) / 1.24 + 1.01 ( C _A / C _B )

The symbols used in the following equations are defined.

Electrical Conductivity of A-Position Contact Medium: C _A

B Conductivity of the contact medium: C _B

Amount of target nucleic acid added: W _{t, total}

Amount of probe complementary to the target nucleic acid: W _{p, total}

Amount of target nucleic acid hybridized with probe: W _{t, ds}

Amount of probe hybridized with target nucleic acid: W _{p, ds}

The amount of target nucleic acid present in a single strand: W _{t, s}

The amount of probe present in a single strand: W _{p, s}

Conductivity per unit amount of single-stranded target nucleic acid: D _{t, s}

Electrical conductivity per unit amount of single strand probe nucleic acid: D _{p, s}

Conductivity per unit amount of hybridized target nucleic acid: D _{t, ds}

Conductivity per unit amount of hybridized probe nucleic acid: D _{p, ds}

As shown in FIG. 2, when a single strand of target nucleic acid is simultaneously applied to two points A and B, the electric conductivities of the two points A and B can be predicted as follows. First, the reference solution in which the nucleic acid hybridization reaction will occur is basically an electrolyte, which may affect the position of the contact signal. Therefore, when the electrical conductivity of this reference solution is defined as E , the nucleic acid detection sample is simultaneously applied to the reference solution (A) and the solution (B) containing the probe complementary to the target nucleic acid, whereby a single stranded target nucleic acid And at point B, a single strand of probe, a single strand of target nucleic acid, and a target nucleic acid hybridized with probe are present. The electrical conductivity ( C _A ) at point _A can be expressed as the sum of the electrical conductivity ( W _{t, total} D _{t, s} ) of the single-strand target nucleic acid and the electrical conductivity ( E ) of the reference solution (equation 2) the electrical conductivity (C _B) the electric conductivity _{_{(W p, s D p,}} s), a single-stranded target nucleic acid electric conductivity by _{_{(W t, s D t,}} s), the hybridized double-stranded nucleic acids according to the single-stranded probe ( W _{t, ds} D _{t, ds} ) and the electrical conductivity ( E ) of the reference solution (Equation 3).

(Equation 2) C _A = W _{t, total} D _{t, s} + E

(Equation 3) C _B = W _{p, s} D _{p, s} + W _{t, s} D _{t, s} + W _{t, ds} D _{t, ds} + E

The amount (W) of the nucleic acid used in this formula is the weight (ng), and the amount of the entire target nucleic acid and the total amount of the probe nucleic acid are the sum of the amount of the nucleic acid of the single strand and the half amount of the double strand nucleic acid. .

(Equation 4) W _{t, total} = W _{t, ds} / 2 + W _{t, s}

(Equation 5) W _{p, total} = W _{p, ds} / 2 + W _{p, s} = W _{t, ds} / 2 + W _{p, s}

On the other hand, since the electrical conductivity ( E ) of the reference solution, the electrical conductivity ( D _{t, s} ) per unit amount of the target nucleic acid, and the electrical conductivity ( D _{t, ds} ) ( D _{p, s} ) per unit volume of the single-stranded probe, another constant.

(Equation 6) E = a W _{p, total} D _{p, s}

(Equation 7) D _{t, s} = b D _{p, s}

(Equation 8) D _{t, ds} = c D _{p, s}

Here, if the hybridization efficiency of the probe nucleic acid and the target nucleic acid is defined as h (0? H ? 1), h can be expressed as follows according to the amount of the probe nucleic acid and the target nucleic acid.

First, when the amount of target nucleic acid applied is less than the amount of probe nucleic acid at point B ( W _{t, total} ≤ W _{p, total} ), the hybridization efficiency h is expressed as the ratio of the amount of double- .

(Equation 9) W _{t, ds} / 2 W _{t, total} = h ∴ W _{t, ds} = 2 h W _{t, total}

Thus, the amount of single-stranded target nucleic acid can be expressed from Equation 4 as follows.

(Equation 10) W _{t, s} = W _{t, total} - W _{t, ds} / 2 = (1 - h ) W _{t, total}

The amount of single-stranded probe nucleic acid can be expressed as follows from the equation (5).

(Equation 11) W _{p, s} = W _{p, total} - W _{t, ds} / 2 = W _{p, toal} - h W _{t, total}

Taking this into account, the electrical conductivity ( C _A ) at point _A can be expressed from Eqs. 2, 6, 7 as follows.

(Equation 12) C _A = b W _{t, total} D _{p, s} + a W _{p, total} D _{p, s}

The electric conductivity ( C _B ) at the point _B can be expressed as shown in the following formulas (3), (6), (7) and (8).

(Equation 13) C _B = W _{p, s} D _{p, s} + b W _{t, s} D _{p, s} + c W _{t, ds} D _{p, s} + a W _{p, total} D _{p, s}

Therefore, A, B two ratio (C _A / C _B) of the electrical conductivity of a point can be expressed as the reduction of a fraction by D _{p, s.}

(14) C _A / C _B = ( b W _{t, total} + a W _{p, total} ) / ( W _{p, s} + b W _{t, s} + c W _{t, ds} + a W _{p, total} )

( C _A / C _B ) at two points of A and B when the amount of target nucleic acid is less than the amount of probe nucleic acid ( W _{t, total} ≤ W _{p, total} ) Are summarized as follows.

(Equation 15) C _A / C _B = ( b W _{t, total} + a W _{p, total} ) / ( W _{p, total} - h W _{t, total} ) + b (One - h ) W _{t, total} + 2 c h W _{t, total} + a W _{p, total}

= ( b W _{t, total} + a W _{p, total} ) / (1 + a ) W _{p, total} - h (1 + b - 2 c ) W _{t, total} ) + b W _{t, total}

Secondly, the hybridization efficiency h can be expressed as the ratio of the amount of the double-stranded nucleic acid in the _total amount of the probe nucleic acid when the amount of the target nucleic acid is greater than the amount of the probe nucleic acid ( W _{t, total} ≥ W _{p, total} ).

(Expression 16) W _{t, ds} / 2 W _{p, total} = h ∴ W _{t, ds} = 2 h W _{p, total}

(Equation 17) W _{t, s} = W _{t, total} - W _{t, ds} / 2 = W _{t, toal} - h W _{p, total}

(Eq. 18) W _{p, s} = W _{p, total} - W _{t, ds} / 2 = (1 - h ) W _{p, total}

Substituting in this synthesis, when the amount of the target nucleic acid is greater than the amount of the probe nucleic acid (W _{t, total} ≥ W _{p, total)} ratio (C _A / C _B) of the electrical conductivity of the two points A, B in the formula 14 , 17, 18 can be expressed as follows.

(Expression 19) C _A / C _B = ( b W _{t, total} + a W _{p, total} ) / ( W _{p, s} + b W _{t, s} + c W _{t, ds} + a W _{p, total} )

= ( b W _{t, total} + a W _{p, total} ) / (One - h ) W _{p, total} + b ( W _{t, total} - h W _{p, total} ) + 2 c h W _{p, total} + a W _{p, total}

= ( b W _{t, total} + a W _{p, total} ) / (1 + a ) W _{p, total} - h (1 + b - 2 c ) W _{p, total} + b W _{t, total}

Therefore, from the position of the signal which appears at the same time by adding the same amount of target nucleic acid to the point A containing only the reference solution and the point containing the probe nucleic acid complementary to the target nucleic acid, the electrical conductivity of the two points A and B You can get the rain. Then, the amount of the probe (W _{p, total)} is because the value known, the constant a, b, just confirmed c can calculate the amount of the target nucleic acid applied in accordance with equation 15 or formula 19 and formula 1 (W _{t, total)} have.

That is, when the constants a , b , and c are determined using the reference sample, the ratio of the electrical conductivity can be derived, and the graph of the signal position can be determined by substituting this into the equation 1 (FIG. The presence or concentration of the target substance present in the sample can be determined by comparing the position of the signal measured using the graph with the signal position of the target substance or the expected signal position of the non-target substance.

In the embodiment of the present invention, a surface capacitance type touch screen (ESCAP7000, eGALAX) including a touch panel and a touch controller is used as the capacitive touch screen. However, the present invention is not limited to this, Either is available.

In an embodiment of the present invention C using asymmetric polymerase chain reaction. trachomatis The target and probe nucleic acids were obtained from the gene (FIG. 5), and the contact signal positions were measured using the reference samples containing the same (FIG. 6), and the constants a, b and c were determined using Equation 15 and Equation 19, (Fig. 7, dotted line), when the target nucleic acid and the probe are combined with each other as a result of the measurement of the contact signal position using the actual target nucleic acid and the non-target nucleic acid, the contact signal position (Fig. 7, filled circles).

According to another aspect of the present invention, there is provided a method for manufacturing a touch panel, comprising the steps of: (a) stacking an analytical frame including a sample inlet, a sample transfer channel, a sample contact position and a refill film on a touch panel of a surface capacitance type touch screen, (b) an auxiliary panel coated with a transparent electrode or a transparent electrode coated on both sides to which leads are connected, is laminated on the analysis frame of (a), and a surface coated with a transparent electrode is laminated on the analysis frame Laminating them so as to contact; (c) injecting a target substance and a probe to be detected into the sample injection port; (d) contacting the touch conductor with a conductor connected to the transparent electrode or a transparent electrode on the opposite side in contact with the touch panel to cause a current to flow on the surface of the touch panel; And (e) a step of detecting a target material by measuring a current with respect to one contact signal position and a contact signal position with a touch controller further comprising an analog signal output element for measuring and outputting a current of the touch panel And more particularly, to a method of detecting a target substance using a capacitive touch screen.

When a target substance containing other residues such as minerals is detected, the target substance to be detected necessarily requires a purification process because the detection result is highly influenced by other residues. Therefore, by using a non-conductive analytical frame that includes a sample inlet, a sample transport channel, a sample contact position, and a purification membrane that separates the target material from other residues, It is possible to detect a target substance (Fig. 9).

In the present invention, the target material may be any biomolecule capable of changing the electrical conductivity of the touch panel depending on whether it is bonded. Preferably, the target material is a group consisting of nucleic acid, protein, inorganic ion in the living body, Wherein the probe is selected from the group consisting of a nucleic acid, a protein, and a mixture thereof capable of binding with a target substance to change electrical conductivity.

In the present invention, the touch conductor may be selected from the group consisting of a finger, a stylus pen applicable to an electrostatic capacity type, and a touch glove applicable to an electrostatic capacity type.

The present invention, in another aspect, relates to a capacitive touch screen in which a sample containing a target material is dropped; And an auxiliary panel coated with a transparent electrode on the both sides thereof, the auxiliary panel being coated with a transparent electrode to which a wire to which the touch conductor is connected is connected.

In the present invention, the surface-capacitance-type touch screen may include a touch panel and a touch controller. The touch controller may further include an analog signal output device for measuring and outputting a current of the touch panel .

In the present invention, the target substance detection apparatus may further include an analysis frame which is not conductive, and the analysis frame includes a sample inlet, a sample movement channel, a sample contact position, and a purification membrane .

Hereinafter, the present invention will be described in more detail with reference to Examples. It will be apparent to those skilled in the art that these embodiments are for illustrative purposes only and that the scope of the present invention is not construed as being limited by these examples.

[Example 1] Chlamydia trachomatis Diagnosis of the resulting target nucleic acid

1-1. Amplification and purification of probe and target nucleic acid

Since the present invention is based on the difference in electrical conductivity between a single-stranded nucleic acid and a double-stranded nucleic acid, the target nucleic acid to be detected must be applied to the touch panel in a single-stranded state. For this, asymmetric PCR was performed from the C. trachomatis gene extracted from actual clinical samples to separate amplified single-stranded nucleic acids by electrophoresis, and nucleic acid was isolated through a gel-extraction kit 5).

PCR primer sequence 1 (forward): 5'-CCATCTTCTTTGAAGCGTTGT

PCR primer sequence 2 (reverse): 5'-ACAGGATGACTCAAGGAATAG

Through asymmetric polymerase chain reaction, 50 pmole forward primer and 0.5 pmole reverse primer were used to obtain the target nucleic acid, and 0.5 pmole forward primer and 50 pmole reverse primer were used to obtain the capture probe.

1-2. Determination of constant values for target material detection using surface capacitive touch screen

10 μL of reference solution (50 μM PBS) was added to point A on the surface-capacitive touch-screen panel to obtain the constant " a " of Eq. 15 or 19 and 10 μL of reference solution μL), and then contacted with two points at the same time, it was confirmed that the signal position L was 0.76 (FIG. 6 (a)).

The ratio of the electrical conductivities ( C _A / C _B ) at the two points A and B is determined to be 0.46 when there is a reference solution at point A, and a single probe and reference solution exist at point B C _A / C _B can be expressed as:

(Equation 20) C _A / C _B = E / ( W _{p, total} D _{p, s} + E ) = 0.46

In summary,

(Expression 21) E = 0.86 W _{p, total} D _{, p, s}

Thus, it was confirmed that the constant a in the equation (6) was determined to be 0.86.

To obtain the constant ' b ' in Eq. 15 or 19, a reference solution (10 μL) in which 200 ng of probe nucleic acid was dissolved at point A on a surface-capacitive touch-screen panel and 200 ng of a target nucleic acid dissolved in B When the reference solution (10 μL) was added, and the two points were simultaneously contacted, it was confirmed that the signal position L was 0.5 (FIG. 6 (b)).

( C _A / C _B ) of the two points A and B is 1, and there is a single-stranded probe nucleic acid and reference solution at point A, and a single-stranded target Since the nucleic acid and the reference solution are present, C _A / C _B can be expressed as follows.

(Equation 22) C _A / C _B = ( W _{p, s} D _{p, s} + E ) / ( W _{t, s} D _{t, s} + E ) = 1

Since the amount of probe and the amount of target are the same,

(Equation 23) D _{t, s} = D _{p, s}

Thus, it was confirmed that the constant b in equation (7) was determined to be 1.

To obtain the constant 'c' of Eq. 15 or 19, a reference solution (10 μL) in which 200 ng of single-stranded target nucleic acid was dissolved at point A on a surface-capacitive touch-screen panel and 200 ng of probe (10 μL) in which the double nucleic acid hybridized with the target nucleic acid was dissolved was added, and the two points were contacted simultaneously. It was confirmed that the signal occurred at 0.6 point between the two points of A and B (FIG. 6 )).

( C _A / C _B ) of the two points A and B is determined to be 0.75, and there is a single-stranded target nucleic acid and reference solution at point A, and a double-stranded target Since the nucleic acid and the reference solution are present, C _A / C _B can be expressed as follows.

(Equation 24) C _A / C _B = ( W _{t, s} D _{t, s} + E ) / ( W _{t, ds} D _{t, ds} + E ) = 0.75

When the equations 21 and 23 are substituted,

(Equation 25) D _{t, ds} = 1.60 D _{p, s}

Thus, it was confirmed that the constant c in the equation (8) was determined to be 1.60.

1-3. Detection of target material using surface capacitive touch screen

To detect the target nucleic acid, 5 μL of the reference solution was added to the A-point of the touch screen panel, and 200 μg of the reference nucleic acid ( W _{p, total} = 200) . 5 μL of the sample to be detected was added to each of the two points A and B and an auxiliary panel coated with a transparent electrode was placed on the transparent electrode so that the transparent electrode was in contact with the sample. And the position of contact of one point between two points was confirmed. When the amount of target nucleic acid added is X ( W _{t, total} = X), the ratio of the electrical conductivities at two points A and B can be represented by the following two cases.

When the amount of the target nucleic acid in the added sample is smaller than the amount of the probe nucleic acid (200 ng), the equations (21), (23), and (25) are substituted into Equation (15)

(Equation 26) C _A / C _B = (X + 172) / 372 + (1 + 1.2 h ) X

When the amount of the target nucleic acid in the added sample is larger than the amount (200 ng) of the probe nucleic acid, it can be understood that the equations 21, 23 and 25 are substituted into Equation 19 as follows.

(Equation 27) C _A / C _B = (X + 172) / 372 + 240 h + X

Here, when the value 0 or 1 is substituted for the hybridization efficiency h, the graph of FIG. 7 can be obtained from the equation 1. The hybridization efficiency 1 indicates that the nucleic acid in the added sample is 100% hybridized with the probe, that the added nucleic acid is the target nucleic acid (FIG. 7b), and the hybridization efficiency 0 is the case where the nucleic acid in the sample does not hybridize with the probe and exists as a single strand , Meaning that the nucleic acid in the sample is not a target nucleic acid (Fig. 7A).

Actually, the detection test was performed with a nucleic acid which was not complementary to the probe and the complementary nucleic acid, and it was confirmed that the results were consistent with the results predicted by the method proposed by the present invention (FIG. 7).

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

1. Finger
2. Current flow through the mediator solution at two locations, generated by contact
3. Touch panel
4. Transparent auxiliary panel coated with electrodes connected to both sides
5. Contact medium solution
6. Display
7. Contact signals appearing between two points by simultaneous contact of the mediator solution at two points
8. Sample inlet
9. Sample contact location
10. Tablet membrane
11. Reference sample contact location
12. Cable
13. Touch controller
14. Analog signal output element
15. Analysis frame

Claims

A method of detecting a target substance using a surface-capacitance touch screen including the steps of:
(a) applying a probe complementary to a reference solution and a target material to two points on a touch panel of a surface capacitance type touch screen;
(b) hybridizing the sample containing the target substance by applying the same amount to each of the two sites;
(c) an auxiliary panel coated with a transparent electrode coated with a conductive wire or coated with a transparent electrode on both sides, is laminated on the touch panel of the above (a) so that the surface coated with the transparent electrode contacts the touch panel Stacking;
(d) contacting the touch conductor with a conductor connected to the transparent electrode or a transparent electrode on the opposite side in contact with the touch panel to cause a current to flow on the surface of the touch panel; And
(e) detecting a target substance by measuring one contact signal position using a touch controller.

A method of detecting a target material using a surface-capacitance touch screen including the steps of:
(a) applying a probe complementary to a reference solution and a target material to two points on a touch panel of a surface capacitance type touch screen;
(b) hybridizing the sample containing the target substance by applying the same amount to each of the two sites;
(c) an auxiliary panel coated with a transparent electrode coated with a conductive wire or coated with a transparent electrode on both sides, is laminated on the touch panel of the above (a) so that the surface coated with the transparent electrode contacts the touch panel Stacking;
(d) contacting the touch conductor with a conductor connected to the transparent electrode or a transparent electrode on the opposite side in contact with the touch panel to cause a current to flow on the surface of the touch panel; And
(e) a step of detecting a target material by measuring a current to one contact signal position and a contact signal position with a touch controller further including an analog signal output element for measuring and outputting a current of the touch panel;

The method of claim 1 or 2, wherein the reference solution is an electrolyte solution having a known concentration.

3. The method of claim 1 or 2, wherein the target material is selected from the group consisting of nucleic acids, proteins, inorganic ions in vivo, and mixtures thereof.

The method of claim 1 or 2, wherein the probe is selected from the group consisting of a nucleic acid, a protein, and a mixture thereof capable of binding with a target substance to change electrical conductivity.

The method of claim 1 or 2, wherein the subpanel is selected from the group consisting of glass, acrylic and plastic.

The organic electroluminescent device according to claim 1 or 2, wherein the transparent electrode is made of indium-tin-oxide (ITO), zinc-oxide (ZnO), indium-zinc- oxide (IZO), gallium- Wherein the material is selected from the group consisting of zinc oxide (AZO) and carbon nanotubes (CNT) or graphene.

The method of claim 1 or 2, wherein the touch conductor is selected from the group consisting of a finger, a stylus pen applicable to an electrostatic capacity type, and a touch glove applicable to an electrostatic capacity type.

3. The method of claim 1 or 2, wherein the step (e) comprises: calculating a distance between a contact signal position on the touch panel measured using the touch controller and a reference solution position applied on the touch panel, (L) of the distance is calculated, and then the ratio (L) of the distance is substituted into the equation prepared using the reference sample containing the target material to be detected, and the ratio of the electric conductivity is calculated, And determining whether hybridization of the probe is detected.

A method of detecting a target material using a surface-capacitance touch screen including the steps of:
(a) stacking an analytical frame including a sample inlet, a sample transfer channel, a sample contact position, and a refining film on a touch panel of a touch screen of a surface capacitance type and not exhibiting conductivity;
(b) an auxiliary panel coated with a transparent electrode or a transparent electrode coated on both sides to which leads are connected, is laminated on the analysis frame of (a), and a surface coated with a transparent electrode is laminated on the analysis frame Laminating them so as to contact;
(c) injecting a target substance and a probe to be detected into the sample injection port;
(d) contacting the touch conductor with a conductor connected to the transparent electrode or a transparent electrode on the opposite side in contact with the touch panel to cause a current to flow on the surface of the touch panel; And
(e) detecting a target material by measuring a current with respect to one contact signal position and a contact signal position with a touch controller further including an analog signal output element for measuring and outputting a current of the touch panel;

11. The method of claim 10, wherein the target material is selected from the group consisting of nucleic acids, proteins, inorganic ions in vivo, and mixtures thereof.

11. The method of claim 10, wherein the probe is selected from the group consisting of a nucleic acid, a protein, and a mixture thereof capable of binding with a target substance to change electrical conductivity.

11. The method of claim 10, wherein the subpanel is selected from the group consisting of glass, acrylic, and plastic.

11. The method of claim 10, wherein the transparent electrode is selected from the group consisting of indium-tin-oxide (ITO), zinc-oxide (ZnO), indium-zinc-oxide (IZO), gallium-zinc- oxide (GZO), aluminum- Wherein the target substance is selected from the group consisting of AZO, carbon nanotubes (CNT), and graphene.

11. The method of claim 10, wherein the touch conductor is selected from the group consisting of a finger, a stylus pen applicable to an electrostatic capacity type, and a touch glove applicable to an electrostatic capacity type.

A capacitive touch screen in which a sample containing a target material is dispensed; And an auxiliary panel coated with a transparent electrode on both sides or coated with a transparent electrode to which a wire to which a touch conductor is connected is connected.

The apparatus of claim 16, wherein the capacitive touch screen includes a touch panel and a touch controller.

18. The apparatus according to claim 17, wherein the touch controller further comprises an analog signal output element for measuring and outputting a capacitance change amount of the touch panel.

17. The apparatus of claim 16, wherein the target material is selected from the group consisting of nucleic acids, proteins, inorganic ions in vivo, and mixtures thereof.

17. An apparatus according to claim 16, wherein said sub-panel is selected from the group consisting of glass, acrylic and plastic.

17. The method of claim 16, wherein the transparent electrode is selected from the group consisting of indium-tin-oxide (ITO), zinc-oxide (ZnO), indium-zinc-oxide (IZO), gallium-zinc- oxide (GZO), aluminum- AZO and carbon nanotubes (CNTs) or graphenes. The device of claim 1,

17. An apparatus according to claim 16, wherein the apparatus for detecting a target substance further comprises an analysis frame which is not conductive.

23. The apparatus according to claim 22, wherein the analysis frame includes a sample inlet, a sample transfer channel, a sample contact position, and a purification membrane.