KR20160128945A - Apparatus for analyzing cartridge detecting target material and Method using thereof - Google Patents

Abstract

The present invention relates to a target material detection cartridge analyzer and a target material detection cartridge analyzing method using the same, capable of having a highly reliable result. According to an embodiment of the present invention, the target material detection cartridge analyzer comprises: a housing which includes a first storage chamber having an open upper part and accommodating a first liquid substance, a second storage chamber being positioned on the upper part of the first storage chamber, being connected with the first storage chamber having open upper and lower parts, and accommodating a second liquid substance, a first separation film being arranged between the upper part of the first storage chamber and the lower part of the second storage chamber and mutually separating the first liquid substance from the second liquid substance, a perforating body forming a hole in the first separation film by passing through the second storage chamber in the upper part of the second storage chamber, and a light receiving sensor being arranged to face the first storage chamber, sensing chemiluminescence generated in the first storage chamber, and measuring an intensity of light, accommodates a target material detection cartridge having at least one section capable of being mixed with the first liquid substance as the second liquid substance flows into the first storage chamber through a hole of the first separation film perforated by the perforating body, and blocks external light from the target material detection cartridge; a storage box arranged on one side of the housing, storing the target material detection cartridge; a push unit positioned inside the housing, drilling a hole in the first separation film by pushing the perforating body of the target material detection cartridge when closing the storage box of storing the target material detection cartridge; a motor which pushes the push unit downwards; and a control unit which controls driving of the motor, receives light intensity data measured by the light receiving sensor, and calculates the result.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a cartridge for analyzing a target material,

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a target substance detection cartridge analysis apparatus and method, and more particularly, to a target substance detection cartridge analysis apparatus and analysis method capable of analyzing signals generated in a subject cartridge.

This research was supported by the Ministry of Commerce, Industry and Energy, the Ministry of Commerce, Industry and Energy, and the project for cooperation with the Ministry of Commerce, Industry and Energy (2013, development and commercialization of arrhythmia diagnosis / treatment equipment, Project No. R0002625).

Recently, there is an increasing demand for on-site diagnostics to directly measure the concentrations of the components to be detected at the site of use and to immediately reflect the results. Particularly, specific needs for diagnostic cartridges and biochips capable of quickly and easily diagnosing and analyzing human diseases are increasing in our everyday and medical fields.

Accordingly, there has been actively developed a detection cartridge for a subject to easily and quickly diagnose and analyze a biological sample. However, in the case of a cartridge for detecting a sample, a signal having a large deviation according to an external environment or a condition is generated. Therefore, there is a need for an analyzing apparatus or an analyzing method capable of correcting and analyzing a signal in order to obtain a reliable result.

SUMMARY OF THE INVENTION The present invention has been made in order to solve the above-mentioned problems, and it is an object of the present invention to provide a target substance detection cartridge analysis device capable of correcting signal values of a subject detection cartridge which generates different signal values according to external environment or conditions, And a method for analyzing a target substance detection cartridge using the same.

An apparatus for analyzing a target substance detection cartridge according to an aspect of the present invention includes: a first reservoir chamber having an open top and receiving a first liquid material; a second reservoir chamber located at an upper portion of the first reservoir chamber, And a second reservoir chamber in which a lower portion is opened and a second liquid material is accommodated, an upper portion of the first reservoir chamber and a lower portion of the second reservoir chamber, the first liquid material and the second liquid material A perforated body passing through the second reservoir chamber at the top of the second reservoir chamber and capable of puncturing the first separator membrane, and a second reservoir chamber disposed to face the first reservoir chamber, Wherein the second liquid material is introduced into the first storage chamber through a hole of the first separator pierced by the perforated body so that the first liquid At least one housing for receiving a cartridge having the target material is detected that the sections that can be mixed with the material, and blocks external light from the detection target material cartridge; A housing provided on one side of the housing and capable of receiving the target material detection cartridge; A push portion located inside the housing and pushing a puncture body of the target material detection cartridge to puncture the first separating membrane when the receptacle containing the target material detecting cartridge is closed; A motor for pushing the push portion downward; And a control unit for controlling driving of the motor, receiving the light intensity data measured by the light receiving sensor, and calculating the result.

The receptacle may be opened or closed from the housing in the form of a drawer.

The push portion includes a plate and a push bar provided at a position corresponding to the perforated body on the plate, the plate being pressed by driving the motor and the push bar being able to push the perforated body.

The push bar further includes a spring surrounding the push bar, and when the pushing force of the plate is reduced, the push bar can be restored to its original position.

Wherein the target material detection cartridge further comprises: a third storage chamber located above the second storage chamber and connected to the second storage chamber, the third storage chamber having an upper surface and a lower surface opened and receiving a third liquid material; And a second separation membrane located between the upper portion of the second storage chamber and the lower portion of the third storage chamber and separating the second liquid material and the third liquid material from each other, So that the first separator and the second separator can be punched.

Wherein the target substance detection cartridge analyzer further comprises a database for storing the luminous intensity data of the chemiluminescence of the reference sample according to the condition, and the control unit is operable to condition the measured luminous intensity of the sample including the target material in conjunction with the database And can be calibrated and calculated accordingly.

And a display unit for displaying results calculated by the control unit on one surface.

According to another aspect of the present invention, there is provided a method for analyzing a target material detection cartridge, including: a first reservoir chamber having an open top and receiving a first liquid material; a second reservoir chamber disposed above the first reservoir chamber, And a second reservoir chamber in which a lower portion is opened and a second liquid material is accommodated, an upper portion of the first reservoir chamber and a lower portion of the second reservoir chamber, the first liquid material and the second liquid material A perforated body passing through the second reservoir chamber at the top of the second reservoir chamber and capable of puncturing the first separator membrane, and a second reservoir chamber disposed to face the first reservoir chamber, Wherein the second liquid material is introduced into the first storage chamber through a hole of the first separation membrane punctured by the perforated body, The material and the process for storing a section which may be mixed target material detection cartridge having at least one holder in the target material detection cartridge analyzer; A step of pushing the perforated body of the target material detection cartridge by the push portion by driving the motor of the target material detection cartridge analyzing device to puncture the first separating membrane when the receptacle is closed; A step of measuring the luminous intensity of the chemiluminescence of the mixture by the light receiving sensor of the target material detection cartridge when the second liquid material is mixed with the first liquid material through the hole of the first separation membrane to generate the mixture; And analyzing the light intensity data measured by the light receiving sensor by the control unit of the target material detection cartridge analysis apparatus.

A method for analyzing a target material detection cartridge according to an embodiment of the present invention includes: a first storage chamber having an open top and receiving a first liquid material; a second storage chamber positioned above the first storage chamber and connected to the first storage chamber A second reservoir chamber in which the upper surface and the lower surface are opened and another first liquid material is accommodated, a second reservoir chamber located above the second reservoir chamber and connected to the second reservoir chamber, A third storage chamber, a first separation membrane disposed between the upper portion of the first storage chamber and the lower surface of the second storage chamber for separating the first liquid material and the second liquid material from each other, A second separation membrane disposed between the upper portion of the third storage chamber and the lower surface of the third storage chamber for separating the second liquid material and the third liquid material from each other, I can make a hole in And a section including at least one section including a perforated body and a light receiving sensor arranged to face the first storage chamber and sensing chemiluminescence generated in the first storage chamber, Providing a second liquid material in the second reservoir chamber and providing a third liquid material in the third reservoir chamber to accommodate the target material detection cartridge in a receptacle of the target material detection cartridge analysis apparatus; When the receptacle is closed, pushing the puncture body of the target material detection cartridge by driving the motor of the target material detection cartridge analyzer to puncture the second separation membrane; When the third liquid material is mixed with the second liquid material through the hole of the second separation membrane to generate the first mixture, the push portion pushes the porous material of the target material detection cartridge to puncture the first separation membrane process; Wherein when the first mixture is mixed with the first liquid material in the first storage chamber through the orifice of the first separation membrane to produce a second mixture, the intensity of the chemiluminescence generated in the second mixture, A process of measuring the light receiving sensor of the detection cartridge; And analyzing the light intensity data measured by the light receiving sensor by the control unit of the target material detection cartridge analysis apparatus.

Wherein the control unit of the target material detection cartridge analysis apparatus analyzes the luminous intensity data measured by the light receiving sensor by using the luminous intensity of the reference sample measured by the light receiving sensor, The conditions at the time of measurement can be compared and the measured luminous intensity data of the sample containing the target material can be calculated by correcting it according to the condition at the time of measurement.

According to an embodiment of the present invention, a target substance detection cartridge analysis device capable of correcting signal values of a detection cartridge for generating a signal value according to an external environment or a condition to obtain a reliable result, and a target material detection A cartridge analysis method can be provided.

1 and 2 are perspective views of a target material detection cartridge analysis apparatus according to an aspect of the present invention, respectively.
Fig. 3 is a perspective view of a target material detection cartridge housed in a receptacle, and Fig. 4 is a schematic sectional view showing an action mechanism of a target material detection cartridge. Fig.
5 is a schematic view showing a push portion provided inside a housing of a target substance detection cartridge analysis apparatus according to an embodiment of the present invention;
6 is a top view of an open housing of a target material detection cartridge analysis apparatus according to an embodiment of the present invention.
7 is a rear view of an open housing of a target material detection cartridge analysis apparatus according to an embodiment of the present invention.
8 is a flowchart of a method for analyzing a target material detection cartridge according to another aspect of the present invention.
9 is a flowchart of a method for analyzing a target material detection cartridge according to another embodiment of the present invention.
FIG. 10 is a graph showing a change in the amount of chemiluminescence emitted from the detection cartridge according to the present invention. FIG.

Hereinafter, the embodiments disclosed herein will be described in detail with reference to the drawings.

I want to explain. Unless otherwise indicated in the text, like reference numerals in the drawings

Represent similar components. In the detailed description, drawings and claims,

The poetic embodiments are not intended to be limiting, and other embodiments may be utilized, and other variations are possible without departing from the spirit or scope of the disclosed technique. Those skilled in the art will appreciate that the components of the present disclosure, that is, the components generally described herein and illustrated in the figures, may be arranged, arranged, combined, or arranged in a variety of different configurations, all of which are expressly contemplated, As shown in FIG. In the drawings, the width, length, thickness or shape of an element, etc. may be exaggerated in order to clearly illustrate the various layers (or films), regions and shapes.

When a component is referred to as being " deployed "to another component, it may include the case where the component is directly disposed on the other component, as well as the case where additional components are interposed therebetween.

The description of the disclosed technique is merely an example for structural or functional explanation and the scope of the disclosed technology should not be construed as being limited by the embodiments described in the text. That is, the embodiments are to be construed as being variously embodied and having various forms, so that the scope of the rights of the disclosed technology should be understood to include equivalents capable of realizing technical ideas.

When an element is referred to as being "connected" to another element, it may be directly connected to the other element, but it should be understood that other elements may be present in between. On the other hand, when an element is referred to as being "directly connected" to another element, it should be understood that there are no other elements in between. On the other hand, other expressions that describe the relationship between components, such as " between " and " between "

It is to be understood that the singular " include " or " have " are to be construed as including the stated feature, number, step, operation, It is to be understood that the combination is intended to specify that it is present and not to preclude the presence or addition of one or more other features, numbers, steps, operations, components, parts or combinations thereof.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the disclosed technology belongs, unless otherwise defined. Terms defined in commonly used dictionaries should be interpreted to be consistent with meaning in the context of the relevant art and can not be construed as having ideal or overly formal meaning unless expressly defined in the present application.

1 and 2 are perspective views of a target material detection cartridge analysis apparatus according to an aspect of the present invention, respectively. Referring to FIGS. 1 and 2, the target substance detection cartridge analysis apparatus includes a housing 20, a housing 10, and a display unit 30. As shown in FIG.

The housing 20 is a structure provided on one side of the housing 10 of the target material detection cartridge analyzing apparatus, preferably on the front side of the housing 10 and can be opened or closed like a drawer from the housing 10 to be described later. The storage box 20 accommodates a target material detection cartridge to be described later to provide a space in which the target material detection cartridge analysis apparatus of the present invention can analyze the target material detection cartridge. The storage box 20 can be provided with a handle 22 so that the door can be easily opened.

Fig. 3 is a perspective view of a target material detection cartridge housed in the receptacle 20, and Fig. 4 is a schematic sectional view showing an action mechanism of the target material detection cartridge.

3 and 4, the target substance detection cartridge includes a first storage chamber 110, a second storage chamber 120, a third storage chamber 130, a first separation membrane 150a, a second separation membrane 150b ), And a perforated body (140).

The third storage chamber 130 is connected to the second storage chamber 120 and the upper and lower ends thereof are opened and the third liquid material 80 is accommodated through a second separation membrane 150b to be described later. For example, the third storage chamber 130 may be connected to the second storage chamber 120 as shown in FIG. In this process, the third storage chamber 130 may be connected to the second storage chamber 120 by a screw coupling method. At this time, the lower end of the third storage chamber 130 is in close contact with the second separation membrane 150b while being connected to the upper end of the second storage chamber 120 in the process of being connected to the second storage chamber 120 in a screw- So that the second liquid material 70 can be received in the second storage chamber 120 in an airtight manner.

The second separation membrane 150b is disposed between the lower end of the third storage chamber 130 and the upper end of the second storage chamber 120 to isolate the third liquid material 80 and the second liquid material 70 from each other . The second liquid material 70 provided in advance in the second storage chamber 120 for diagnosis can be isolated from other materials. On the other hand, the user of the subject detection cartridge disclosed in this specification can remove the third liquid material 80 and the second liquid material 70 through removal or destruction of the second separation membrane 150b May be mixed to produce a first liquid mixed material.

The second storage chamber 120 is connected to the third storage chamber 130 and the first storage chamber 110,

In the opened structure, the second liquid material 70 is accommodated using the first separating film 150a to be described later. 4, the second storage chamber 120 may be formed of a liquid mixture material of the third liquid material 80 and the second liquid material 70 by using the perforated body 140, The lower end of the second storage chamber 120 may be formed in such a shape that its width becomes narrower as it goes down to allow the one liquid mixture material to be easily injected into the first storage chamber 110 in the course of mixing with the first liquid material 60. [ Lt; / RTI >

The first separator 150a is disposed between the lower end of the second reservoir chamber 120 and the upper end of the first reservoir chamber 110 and isolates the second liquid material 70 and the first liquid material 60 from each other . The first liquid material 60 provided in advance in the first storage chamber 110 for diagnosis can be isolated from other materials. On the other hand, the user of the subject detection cartridge disclosed in this specification mixes the first liquid mixture material with the first liquid material 60 through the removal or destruction of the first separation membrane 150a at the time when the detection of the subject is required A second liquid-phase mixed material can be produced. The object of the third liquid material 80 can be detected by measuring the light, heat or electric signal generated in this process.

The first storage chamber 110 is opened at the top, and the first liquid material 10 is accommodated. As shown in FIG. 3, at least one first storage chamber 110 may be formed in the support 160. The first storage chamber 110 serves as a place where mixing of the first liquid material 10, the second liquid material 20 and the third liquid material 30 occurs to generate the second liquid mixture material.

The first liquid material 60 may be supplied and stored in the first storage chamber 110 in a predetermined amount predetermined according to the type, method, or the like of the diagnosis target. For example, the first liquid material 60 may include a sensing material capable of diagnosing and analyzing the third liquid material 80. The third liquid material 80 may contain an analyte for diagnosis. The target substance may be sodium, potassium (K), potassium (K), sodium (K), sodium (K) Blood glucose, specific proteins, toxic substances, various biomarkers, and the like. The second liquid material 70 may include a reaction promoter such as a catalyst or a reaction medium such as an aptamer. The reaction promoter may perform a function of promoting the reaction of the first liquid material 60 and the third liquid material 80 in the process of producing the liquid mixture material. The reaction mediator may act as a mediator when a direct reaction between the first liquid material 60 and the third liquid material 80 is difficult in the process of producing the liquid mixture material. That is, the third liquid material 80 first performs the function of binding with the reaction medium and helping the reaction medium, which is combined with the third liquid material 80, react with the first liquid material 60. In general, various aptamers are used as the reaction mediator. For example, the aptamer may be combined with the target material contained in the third liquid material 80 during the reaction between the aptamer and the third liquid material 80. At this time, the displacement of the electric energy and the displacement of the chemical polarity may occur in the aptamer during the binding process between the target substance and the aptamer. Thereafter, light, heat or electric signals may be generated during the reaction of the aptamer with the first liquid material 10. In other words, depending on the kind of the first liquid material 60, the second liquid material 70 and the third liquid material 80, light, heat or electric signals may be generated in the mixing process of the liquid mixed material. The sensor 170 disposed adjacent to the first storage chamber 110 can sense this. A signal sensed by the sensor 170 can be compared with a preset reference value stored in a storage device (not shown) such as a memory. Whereby the sample analyzing cartridge disclosed herein can diagnose and analyze the third liquid material 80. For example, the sensor 170 senses light generated in the mixing process of the first liquid material 60, the second liquid material 70, and the third liquid material 80 to detect the third liquid material 80 . In this case, the first storage chamber 110 may be made of a light-transmitting material.

The perforated body 140 passes through the interior of the third storage chamber 130 and punctures the second separation membrane 150b and passes through the first separation membrane 150a while passing through the interior of the second storage chamber 120. [ The third liquid material 80 flows into the second reservoir chamber 120 through the hole of the second separator 150b pierced by the perforated body 140 and is mixed with the second liquid material 70 The first liquid mixed material is generated and flows into the first storage chamber 110 via the first separator 150a penetrated by the perforated body 140 to form the first liquid material 60, To form a second liquid-phase mixed material. For example, the perforated body 140 may have a shape in which the width of the perforated body 140 is narrowed with respect to a direction of movement to the first storage chamber 110. In particular, the end of the perforated body 140 may have a pointed shape, and through which the second separation membrane 150b and the first separation membrane 150a can be effectively pierced.

3 and 4 show a sample detection cartridge including a first storage chamber, a second storage chamber 120 and a third storage chamber 130, but the second storage chamber 120 is omitted, A storage chamber and a third storage chamber 130 may also be applied. In this case, the third storage chamber 130 may contain a liquid material in which the test substance and the reaction promoter or the reaction medium are mixed.

Referring again to Figures 1 and 2, the housing 10 is the portion that occupies most of the volume of the target material detection cartridge analysis apparatus of the present invention. It is preferable that the housing 10 completely block the light as a dark room so that external light does not enter into the target material detection cartridge located therein.

5 is a schematic view showing a push portion provided inside the housing 10 of the analyte detection cartridge analysis apparatus according to one aspect of the present invention. 6 is a top view of an open housing 10 of a analyte detection cartridge analyzer according to an aspect of the present invention. 7 is a rear view of the opened housing 10 of the analyte detecting cartridge analyzing apparatus according to an aspect of the present invention. 5 to 7, the push portion 45 includes a motor 41, a rotor 46, a plate 44, and a push bar 42. As shown in Fig. The motor 41 is a driving subject that rotates the rotor 46 connected to the motor 41. The rotor 46 is connected to the motor 41 and rotates. The rotor 46 is preferably a structure having a major axis and a minor axis such as an oval shape or a water droplet shape. When the receptacle 20 with the target material detection cartridge is closed, the long axis portion of the rotor 46 pushes the plate 44 downward as the rotor 46 rotates and the long axis of the rotor 46 rotates downward . So that at least one push bar 42 connected to the plate 44 is pushed downward to push the perforated body 140 provided in each section of the target material detection cartridge. The position of the push bar 42 is provided at a position corresponding to the perforated body 140 of the target material detection cartridge. The push bar 42 is provided with a spring 43. When the plate 44 is pressed by the rotation of the rotor 46 and the push bar 42 is lowered and the pressing force of the plate 44 is reduced, The push bar 42 may be returned to the original position by the restoring force of the push bar 42. [

The control unit 50 controls the driving of the motor 41 of the pushing unit 45 and receives the light intensity data measured by the light receiving sensor and calculates the result. The result calculated by the control unit 50 can be displayed on the display unit 30. [ 1 and 2, the display unit 30 is provided on the top surface of the target substance detection cartridge analyzer, but it is not limited thereto and may be provided on the other surface.

8 and 9 are flowcharts showing the flow of a method for analyzing a target material detection cartridge according to another aspect of the present invention. Referring to FIG. 8, the inspected object detection cartridge is housed in the receptacle 20 of the target material detection cartridge analysis apparatus (S300). Since the structure of the inspection object detection cartridge has been described with reference to FIGS. 3 and 4, detailed description thereof will be omitted.

Next, when the receptacle 20 is closed, the push portion 45 pushes the perforated body 140 of the target substance detection cartridge by driving the motor 41 of the target substance detection cartridge analyzing device, (S310). The rotor 46 connected to the motor 41 rotates and pushes the plate 44 downward. Thus, at least one push bar 42 connected to the plate 44 is lowered to push the perforated body 140 provided in each section of the target material detection cartridge. The position of the push bar 42 is provided at a position corresponding to the perforated body 140 of the target material detection cartridge.

The third liquid material 80 contained in the third reservoir chamber 130 flows into the second reservoir chamber 120 through the hole and flows into the second reservoir chamber 120, Is mixed with the second liquid material (70) stored in the second liquid material (70).

Next, the push portion 45 pushes the perforated body 140 of the target material detection cartridge to puncture the first separator 150a (S315). A mixture of the third liquid material 80 and the second liquid material 70 is mixed with the first liquid material 60 in the first storage chamber through the hole of the first separator 150a.

 When the mixture of the third liquid material 80 and the second liquid material 70 is mixed with the first liquid material 60, the chemiluminescence is caused by the luminescent material contained in the first liquid material 60.

Next, the light-receiving sensor of the target material detection cartridge measures the luminous intensity of chemiluminescence (S320).

Next, the luminous intensity data of the chemiluminescence measured by the light receiving sensor is received and analyzed by the control unit 50 of the target material detection cartridge analysis apparatus (S330).

In this process, the luminous intensity of the chemiluminescence may be varied depending on the measurement environment or conditions such as temperature and humidity even for the sample of the same concentration. In addition, the luminous intensity of the chemiluminescence can be changed in static characteristics and dynamic characteristics depending on the environmental conditions in which the sensor 170 is used, that is, temperature, humidity, etc., even though the sensor 170 used for measurement is the same. In addition, characteristics may be changed depending on the manufacturing process, so that errors such as a sensitivity error, a sensitivity drift, an offset, and zero or null drift due to changes in the surrounding environment Calibration is required. It is necessary to first measure the brightness of the reference sample to calculate the standard value, to measure the brightness of the sample having the test object, to calculate the brightness data of the chemiluminescence with the corrected value, and then to analyze it through the control unit 50. More specifically, first, the errors of the respective sensors 170 used for the measurement of the reference sample and the measurement of the analyte are grasped and corrected. Then, the luminous intensity is measured with respect to the reference sample whose luminous intensity is known in advance according to the measurement environment or condition. Thereafter, the light intensity measured for the reference sample is compared with the chemiluminescence luminous intensity data of the reference sample stored in advance in the database to specify the offset of the sensor 170. Thereafter, the measured light intensity of the sample including the target material is calculated by reflecting the offset of the sensor 170 to the light intensity obtained through the measurement of the sample including the target material, according to the conditions at the time of measurement. Thereafter, the measured luminous intensity of the sample containing the calibrated target material can be compared with the chemiluminescent luminous intensity data of the target material previously stored in the database to analyze the concentration of the target material. In summary, the process of analyzing the light intensity data measured by the light-receiving sensor 170 by the control unit 50 of the target material detection cartridge analyzer is as follows. The control unit 50 compares the luminous intensity of the reference sample measured by the light receiving sensor 170 with the chemiluminescence luminous intensity data of the reference sample stored in advance in the database to specify conditions at the time of measurement. In this process, the offset can be calculated. Thereafter, the measured light intensities of the sample containing the target substance are corrected according to the conditions at the time of measurement, that is, the calculated intensities of the sample containing the target substance are reflected in accordance with the calculated offsets. The concentration of the sample can be analyzed on the basis of the calculated corrected light intensity.

FIG. 10 is a graph showing a change in the amount of chemiluminescence emitted from a sample detection cartridge which changes according to a condition. The difference between the light amounts of the reference sample and the sample containing the target material according to changes in external conditions is stored in a database (not shown) Or can be specified by a formula. The photometric measurement of the chemiluminescence of the reference sample and the sample containing the target material can be performed simultaneously by preparing each sample in each section of the target material detection cartridge having a plurality of sections. Or a single section of the target substance detection cartridge, the reference sample and the sample containing the target substance are separately contained in a plurality of detection cartridges. The luminosity of the chemiluminescence of the reference sample is measured first, and the chemiluminescence intensity of the sample containing the following target substance is measured It can also be measured.

9 is a flowchart of a method for analyzing a target material detection cartridge according to another embodiment of the present invention. In this embodiment, the second storage chamber is omitted in the inspection object cartridge described in Fig. 8, and the first storage chamber and the third storage chamber are connected to each other. Therefore, a detailed description will be omitted since it is similar to the explanation using FIG.

From the foregoing it will be appreciated that various embodiments of the present disclosure have been described for purposes of illustration and that there are many possible variations without departing from the scope and spirit of this disclosure. And that the various embodiments disclosed are not to be construed as limiting the scope of the disclosed subject matter, but true ideas and scope will be set forth in the following claims.

Claims (10)

A first reservoir chamber having an upper portion opened and receiving a first liquid material; a second reservoir chamber located above the first reservoir chamber and connected to the first reservoir chamber, A first separator disposed between the reservoir chamber, an upper portion of the first reservoir chamber and a lower portion of the second reservoir chamber for separating the first liquid material and the second liquid material from each other, A perforated body passing through the second storage chamber to puncture the first separation membrane and a light receiving sensor arranged to face the first storage chamber and detecting chemiluminescence generated in the first storage chamber and measuring the luminous intensity Wherein the second liquid material is introduced into the first reservoir chamber through the orifice of the first separator pierced by the perforator to be mixed with the first liquid material, Receiving a detection cartridge, and the housing to block the external light from the detection target material cartridge;
A housing provided on one side of the housing and capable of receiving the target material detection cartridge;
A push portion located inside the housing and pushing a puncture body of the target material detection cartridge to puncture the first separating membrane when the receptacle containing the target material detecting cartridge is closed;
A motor for pushing the push portion downward; And
And a control section for controlling the driving of the motor, receiving the luminous intensity data of the chemiluminescence measured by the light receiving sensor, and calculating a result. The method according to claim 1,
Wherein the receptacle can be opened and closed from the housing in the form of a drawer. The method according to claim 1,
Wherein the push portion includes a plate and a push bar provided at a position corresponding to the perforated body on the plate so that the plate is pressed by the motor and the push bar pushes the perforated body. . The method according to claim 1,
Further comprising a spring surrounding the push bar, wherein when the pushing force of the plate is reduced, the push bar is returned to its original position. The cartridge according to claim 1, wherein the target material detection cartridge
A third reservoir chamber located above the second reservoir chamber and connected to the second reservoir chamber, the upper surface and the lower surface of the third reservoir chamber being opened, and a third liquid material being accommodated; And
Further comprising a second separator located between an upper portion of the second reservoir chamber and a lower portion of the third reservoir chamber for separating the second liquid material and the third liquid material from each other,
Wherein the pushing portion pushes the puncturing member to puncture the first separating membrane and the second separating membrane. The method according to claim 1,
Wherein the target substance detection cartridge analyzer further comprises a database for storing the luminous intensity data of the chemiluminescence of the reference sample according to the condition, and the control unit is operable to condition the measured luminous intensity of the sample including the target material in conjunction with the database And the target substance detection cartridge is calibrated and corrected. The method according to claim 1,
And a display unit for displaying a result calculated by the control unit on one surface of the target substance detection cartridge. A first reservoir chamber having an upper portion opened and receiving a first liquid material; a second reservoir chamber located above the first reservoir chamber and connected to the first reservoir chamber, A first separator disposed between the reservoir chamber, an upper portion of the first reservoir chamber and a lower portion of the second reservoir chamber for separating the first liquid material and the second liquid material from each other, A perforated body passing through the second storage chamber to puncture the first separation membrane and a light receiving sensor arranged to face the first storage chamber and detecting chemiluminescence generated in the first storage chamber and measuring the luminous intensity Wherein the second liquid material is introduced into the first reservoir chamber through the orifice of the first separator pierced by the perforator to be mixed with the first liquid material, The process of receiving the detection cartridge in the holder of the target material detection cartridge analyzer;
A step of pushing the perforated body of the target material detection cartridge by the push portion by driving the motor of the target material detection cartridge analyzing device to puncture the first separating membrane when the receptacle is closed;
A step of measuring the luminous intensity of the chemiluminescence of the mixture by the light receiving sensor of the target material detection cartridge when the second liquid material is mixed with the first liquid material through the hole of the first separation membrane to generate the mixture; And
A process of analyzing the light intensity data measured by the light receiving sensor and receiving the light intensity data from the control unit of the target material detection cartridge analyzer;
Wherein the target substance detection cartridge is a cartridge. A first reservoir chamber disposed above the first reservoir chamber and connected to the first reservoir chamber and having an upper surface and a lower surface opened and a different first liquid material accommodated therein; A second reservoir chamber, a third reservoir chamber located above the second reservoir chamber and connected to the second reservoir chamber, the third reservoir chamber having an upper surface and a lower surface opened and receiving a third liquid material, A first separation membrane disposed between the lower surface of the first reservoir chamber and the lower surface of the second reservoir chamber for separating the first liquid material and the second liquid material from each other, A second separation membrane for separating the second liquid material and the third liquid material from each other, a perforator capable of piercing the second separation membrane and the first separation membrane on the upper surface of the third storage chamber, And a second storage chamber A first liquid material is provided in the first storage chamber and a second liquid material is provided in the second storage chamber in a target material detection cartridge having at least one section including at least one section including a light receiving sensor for detecting chemiluminescence generated in the second storage chamber And a third liquid material is provided in the third storage chamber to store the target material detection cartridge in a storage box of the target material detection cartridge analysis apparatus;
When the receptacle is closed, pushing the puncture body of the target material detection cartridge by driving the motor of the target material detection cartridge analyzer to puncture the second separation membrane;
When the third liquid material is mixed with the second liquid material through the hole of the second separation membrane to generate the first mixture, the push portion pushes the porous material of the target material detection cartridge to puncture the first separation membrane process;
Wherein when the first mixture is mixed with the first liquid material in the first storage chamber through the orifice of the first separation membrane to produce a second mixture, the intensity of the chemiluminescence generated in the second mixture, A process of measuring the light receiving sensor of the detection cartridge; And
The light intensity data measured by the light receiving sensor is received and analyzed by the control unit of the target material detection cartridge analyzer
Wherein the target substance detection cartridge is a cartridge. 10. The method according to claim 8 or 9,
Wherein the light intensity data measured by the light receiving sensor is received and analyzed by the control unit of the target material detection cartridge analyzer,
The control unit compares the luminous intensity of the reference sample measured by the light receiving sensor with the chemiluminescence luminous intensity data of the reference sample stored in advance in the database to specify the condition at the time of measurement and measures the measured luminous intensity of the sample containing the target material at the time of the measurement Wherein the target substance detection cartridge is calibrated in accordance with the condition of the target substance detection cartridge.

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