KR20160128482A - analyzing system for biological material - Google Patents

Abstract

A biological sample measurement apparatus is disclosed. The biological sample measurement apparatus includes a main body 110 having a first opening 1101 with a space inside, A lid 120 for opening and closing the first opening 1101 of the main body 110; And an actuating part 130 installed in the space provided in the main body part 110. The actuating part 130 includes an installation part 1310 in which a reagent container is installed; A pump unit 1320 provided at one side of the mounting unit 1310 to supply a reagent contained in the reagent vessel to the outside; An injection unit 1340 for injecting the reagent supplied by the pump unit 1320 into the tube; A tube mounting portion provided at a lower portion of the charging portion 1340 and having the tube installed therein; And a first sensor 1355 disposed adjacent to the tube mounting portion. Wherein the reaction material is contained in the tube and the reaction material is mixed with the reagent supplied from the input portion in the tube and the first sensor 1355 is generated by the mixing of the reagent and the reactant The luminescence is measured to determine the concentration of the reagent or the biological sample contained in the reactant.

Description

[0001] The present invention relates to an analyzing system for biological material,

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a biological sample measurement apparatus, and more particularly, to a method and apparatus for measuring a plurality of quantification targets by detecting light emitted through chemiluminescence generated by an antigen- And a biometric sample measuring device.

This research was supported by the Ministry of Commerce, Industry and Energy, the Ministry of Commerce, Industry and Energy, and the project for cooperation with the Ministry of Commerce, Industry and Energy (2013, development and commercialization of arrhythmia diagnosis / treatment equipment, Project No. R0002625).

Recently, there is an increasing demand for on-site diagnostics to directly measure the concentrations of the components to be detected at the site of use and to immediately reflect the results. Particularly, a specific requirement for a biological sample measuring device capable of quickly and easily diagnosing and analyzing human diseases is increasing in daily life and medical field.

A variety of methods can be used to measure biological samples. Among them, there is a chemiluminescence measurement method by measuring chemiluminescence radiating from a chemical reaction process between a biological sample and a reagent. The chemiluminescence assay is widely used for the analysis of biological samples through the measurement of luminescence generated in the antigen-antibody reaction and the reaction process of the target substance and the aptamer.

The antigen-antibody reaction is a very specific reaction in which the antibody acts only with the antigen of interest. That is, an antibody or an antigen that binds to a specific antigen or antibody. When the antibody to be detected is an antigen, a specific antibody is used. In this case, an enzyme is bound to the specific antibody. When an antigen is bound to a specific antibody, the substrate is reacted with the enzyme bound to the specific antibody, so that the antibody is colored and the concentration is measured from the change in color. Currently, antibodies bound to enzymes are widely used for the quantitative analysis of viruses, and enzyme-linked immunosorbent assays (ELISA), which is a representative method thereof, are used. An enzyme-linked antibody assay is a quantification method based on an antigen-antibody reaction using an enzyme as a marker. These analytical methods are useful for assaying antigens from unknown samples or for quantifying antibodies.

1 schematically shows an enzyme-linked antibody assay. As shown in FIG. 1, an antigen 23 acting on the first antibody 21 is attached to a tube 10 to which a first antibody 21 is attached. To bind the antigen (23) to the first antibody (21). The enzyme 27 is conjugated with the second antibody 25 acting on the antigen 23. The second antibody 25 acts on the antigen 23 bound to the first antibody 21 and binds thereto. Then, the second antibody (25) which is not bound to the antigen (23) is washed and removed. A coloring or a light emitting reagent is injected into the tube 10, and the concentration or color development or luminescence is measured.

According to the procedure described above, the concentration of the antibody acting on the antigen or the antigen is measured. However, since there is no automated apparatus for measuring the concentration by adding the coloring or the luminescent reagent, there was an inconvenience in measuring the concentration, There is a problem that can not be continuously measured. This problem also applies to the luminescence measurement generated in the reaction process of the target substance and the aptamer.

It is an object of the present invention to provide a biological sample measuring apparatus capable of automatically measuring a plurality of samples in a short time and analyzing the concentration.

In order to achieve the above object, a biological sample measuring apparatus according to the present invention includes: a main body 110 having a first opening 1101 with a space inside; A lid 120 for opening and closing the first opening 1101 of the main body 110; And an actuating part 130 installed in the space provided in the main body part 110. The actuating part 130 includes an installation part 1310 in which a reagent container is installed; A pump unit 1320 provided at one side of the mounting unit 1310 to supply a reagent contained in the reagent vessel to the outside; An injection unit 1340 for injecting the reagent supplied by the pump unit 1320 into the tube; A tube mounting portion provided at a lower portion of the charging portion 1340 and having the tube installed therein; And a first sensor 1355 disposed adjacent to the tube mounting portion. Wherein the reaction material is contained in the tube and the reaction material is mixed with the reagent supplied from the input portion in the tube and the first sensor 1355 is generated by the mixing of the reagent and the reactant The luminescence is measured to determine the concentration of the reagent or the biological sample contained in the reactant.

The tube installation part includes a rotating body 1380 provided at the lower part of the charging part 1340; And a driving unit 1370 for rotating the rotating body 1380. The rotating body 1380 includes a flange 1381 spaced circumferentially and having a plurality of insertion holes 1382 formed therein; And a body 1381 provided at a lower portion of the flange 1381 and having a plurality of concave recesses 1384 connected to the outer surface thereof by the insertion holes 1382 and having a rotation shaft hole 1385 formed at the center thereof . The driving unit 1370 is connected to the rotating body 1380 through the rotating shaft hole 1385. The tube is fixedly inserted into the inserting hole and the first sensor is fixed to the side of the rotating body 1380 To measure the luminescence produced by the mixing of the reagent and the reactant in the tube.

The main body 110 includes a base plate 1107 and the rotating body 1380 may include a plurality of rotating terminals 1387 spaced apart from each other in a circumferential direction at a lower portion of the main body 1381 . The driving unit 1370 includes a rotation shaft 1371 inserted into the rotation shaft hole 1385; A housing 1373 fixed to the base plate 1107 and rotatably supporting the rotation shaft 1371; A first motor 1379 connected to the rotation shaft 1371; And a third drive sensor 1376 that operates with the rotation terminal 1387. [ The plurality of tubes may be disposed in the plurality of insertion holes 1382, respectively. The rotation terminal 1387 may be provided at the lower portion of the main body 1381 of the rotating body 1380 so as to correspond to the plurality of insertion holes 1382. The first motor 1379 rotates the rotating body 1380 and stops rotating when the third driving sensor 1376 detects the rotating terminal 1387. After the rotation of the rotating body 1380 stops, the introducing unit 1340 injects the reagent into a tube disposed opposite to the first sensor 1355 among the plurality of tubes. The first sensor 1355 may measure the light emission generated by the mixing of the reagent and the reactant in the tube disposed opposite the first sensor 1355 among the plurality of tubes. When the light emission measurement by the first sensor 1355 is completed, the first motor 1379 rotates the rotating body 1380.

A plurality of the reagent containers may be installed in the mounting portion 1310, and the plurality of reagent containers may include a first reagent container, a second reagent container, and a third reagent container. The pump unit 1320 draws the reagent contained in the first reagent vessel and the reagent contained in the second reagent vessel and supplies the reagent to the third reagent vessel to produce a mixed reagent, It can be withdrawn from the reagent vessel and supplied to the outside.

The plurality of reagent vessels include a fourth reagent vessel, and the pump unit 1320 draws a reagent contained in the first reagent vessel and a reagent contained in the second reagent vessel and supplies the reagent to the third reagent vessel And the mixed reagent is withdrawn from the third reagent vessel, and the reagent contained in the fourth reagent vessel is withdrawn and supplied to the outside.

The reactive material may comprise an antigen conjugated with an enzyme and a second antibody, wherein the antigen is associated with a first antibody attached to the tube. The reagent contained in the first reagent vessel and the reagent contained in the second reagent vessel and the other reagent respectively react with an aqueous solution of hydrogen peroxide by the presence of the enzyme to form an electron donor And a luminescent material that reacts with the fluorescent material to emit light. The reagent contained in the fourth reagent vessel contains the hydrogen peroxide, and the light can be emitted through the mixture of the reactant, the reagent, the reagent, and the hydrogen peroxide in the tube .

The enzyme is HRP (Horseradish peroxidase), the electron donor is Amplex-Red (10-acetyl-3,7-dihydroxyphenoxazine), and the luminescent material may be 1,1'-Oxalyldiimidazole.

Wherein the reactive material comprises at least one selected from the group consisting of phenylglyoxal, phenylglyoxal derivative, and combinations thereof,

Wherein the reagent contained in the first reagent vessel comprises any one selected from the group consisting of saliva, blood, urine, and combinations thereof, including sodium (Na) or potassium (K)

The reagent contained in the second reagent vessel reacts with the sodium or potassium and is an aptamer having a DNA or RNA sequence containing guanine,

The reagent contained in the fourth reagent vessel may be tetra-n-methyl ammonium phosphate, tetra-n-ethyl ammonium phosphate, tetra-n-propyl ammonium phosphate (TPA) And may include at least any one selected.

The charging unit 1340 includes at least one guide rod 1343 fixed to the base plate 1107; A sensor rod 1348 fixed to the base plate 1107; A bracket 1341 fixed to the upper portion of the guide rod 1343; A screw 1345 rotatably installed on the bracket 1341 and the base plate 1107; An installation member 1347 which moves up and down along the guide rod 1343 by the rotation of the screw 1345; An upper sensor 1342 and a first tube sensor 1344 installed in the sensor rod 1348; A nozzle provided on the mounting member 1347; And a second motor 1353 connected to a lower portion of the screw 1345 to rotate the screw. The reagent is injected into the tube through the nozzle. The upper sensor senses the rising of the mounting member and the first tube sensor senses the lowering of the mounting member so that the reagent can be injected into the tube through the nozzle at a predetermined position.

The side surfaces of the plurality of mounting grooves 1384 formed concavely in the outer surface of the main body 1381 are formed as mirror surfaces 1386 so that the plurality of Light directed toward the lateral direction of the mounting groove may be reflected on the specular surface to be directed to the first sensor.

The rotating body may be detachably disposed in the driving unit, and the rotating body may include an identification terminal 1389 at a lower portion of the main body. The driving unit 1370 may further include a first driving sensor 1372 or a second driving sensor 1374. The input unit 1340 may further include a second tube sensor 1346 provided below the first tube sensor 1344 in the sensor bar 1348. The rotating body, which is detachably attached to the driving unit, may have various shapes, and the rotating bodies having various shapes may be separated from each other through the identification terminal. The first driving sensor or the second driving sensor may sense the identification terminal to distinguish the rotating body. The second tube sensor senses the lowering of the attachment member and allows the reagent to be injected into the tube through the nozzle at a predetermined position corresponding to the shape of the rotating body.

The measuring apparatus 100 may further include a heating means 180. The heating means 180 includes guide rails 1802 provided at both sides of a second opening formed at one side of the main body 110; A tray 1801 on which a plate is disposed and which is movably installed along the guide rail 1802 through the second opening; And a heating unit installed inside the second opening. The tube is disposed on the plate, and the reaction material accommodated in the tube can be heated to a predetermined temperature by the heating unit.

The mounting portion 1310 includes a lower plate 1311 fixed to the main body 110, A vertical plate 1312 extending upwardly from the lower plate 1311; A plurality of upper plates 1313 extending from the vertical plate 1312 in parallel with the lower plate 1311; A plurality of fixing screws 1317 passing through the lower plate 1311 and the upper plate 1313 to fix the upper plate 1313 to the main body 110; And an elastic body 1318 having both ends fixed to the fixing screw 1317. [ A plurality of recesses 1314 may be formed in the upper plate 1313 between the fixing screws 1317 on which the elastic body 1318 is fixed. The reagent vessel is located in the recess and can be fixed by the elastic body.

And a second sensor disposed on the vertical plate 1312 for detecting whether the reagent is contained in the reagent container.

The foregoing provides only a selective concept in a simplified form as to what is described in more detail hereinafter. The present disclosure is not intended to limit the scope of the claims or limit the scope of essential features or essential features of the claims.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram for explaining an enzyme-linked antibody measurement method among biological sample measurement methods. FIG.
2 is a schematic perspective view showing a biological sample measurement apparatus according to the present invention.
FIG. 3 is a schematic perspective view showing a state in which the cover is opened in the biological sample measurement apparatus of FIG. 2;
4 is a schematic perspective view showing an internal structure of a biological sample measurement apparatus according to the present invention.
5 is a partial plan view of a biological sample measurement apparatus according to the present invention.
6 is a side view in the direction A of Fig.
7 is an enlarged perspective view of a driving unit for driving the rotating body.
8 is a schematic cross-sectional view for explaining the driving unit of FIG.
Fig. 9 is a perspective view showing a state in which tubes are installed in the tube rotating body. Fig.
10 is a sectional view taken along the line AA in Fig.
Fig. 11 is a bottom view of the entirety of Fig. 9; Fig.
12 is a cross-sectional view showing another type of rotating body.
Fig. 13 is a bottom view of the whole of Fig. 12; Fig.
14 is a side view showing an enlarged view of the charging unit.
Fig. 15 is an enlarged view of the heating means.
16 is a structural view of a biological sample measurement apparatus according to the present invention.

Hereinafter, embodiments disclosed in this specification will be described in detail with reference to the drawings. Like reference numerals in the drawings denote like elements, unless the context clearly indicates otherwise. The exemplary embodiments described above in the detailed description, the drawings, and the claims are not intended to be limiting, and other embodiments may be utilized, and other variations are possible without departing from the spirit or scope of the disclosed technology. Those skilled in the art will appreciate that the components of the present disclosure, that is, the components generally described herein and illustrated in the figures, may be arranged, arranged, combined, or arranged in a variety of different configurations, all of which are expressly contemplated, As shown in FIG. In the drawings, the width, length, thickness or shape of an element, etc. may be exaggerated in order to clearly illustrate the various layers (or films), regions and shapes.

When one component is referred to as "connecting to another component ", it includes not only the case where the one component is directly connected to the other component, but also a case where an additional component is interposed therebetween.

It is to be understood that when an element is referred to as " supporting " another element, it may directly support the other element, but there may be other elements in between. On the other hand, when an element is referred to as " directly supporting " another element, it should be understood that no other element is present in between. On the other hand, other expressions that describe the relationship between components, such as " between " and " between "

The description of the disclosed technique is merely an example for structural or functional explanation and the scope of the disclosed technology should not be construed as being limited by the embodiments described in the text. That is, the embodiments are to be construed as being variously embodied and having various forms, so that the scope of the rights of the disclosed technology should be understood to include equivalents capable of realizing the technical ideas.

It is to be understood that the singular " include " or " have " are to be construed as including the stated feature, number, step, operation, It is to be understood that the combination is intended to specify that it is present and not to preclude the presence or addition of one or more other features, numbers, steps, operations, components, parts or combinations thereof.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the disclosed technology belongs, unless otherwise defined. Terms defined in commonly used dictionaries should be interpreted to be consistent with meaning in the context of the relevant art and can not be construed as having ideal or overly formal meaning unless expressly defined in the present application.

Hereinafter, a biological sample measuring apparatus according to a preferred embodiment of the present invention will be described in detail with reference to the drawings. Hereinafter, the operation of a biological sample measuring apparatus is described mainly by a method of measuring an enzyme-linked antibody. It is to be understood that these examples are merely examples for understanding, and other biological sample measurement methods other than the enzyme binding antibody measurement method may be applied to the present measurement apparatus.

FIG. 2 is a perspective view schematically showing a biological sample measurement apparatus according to the present invention, FIG. 3 is a schematic perspective view showing a state in which a cover is opened in the biological sample measurement apparatus of FIG. 2, 5 is a plan view showing a mounting part of the biological sample measurement apparatus according to the present invention, FIG. 6 is a side view in the direction A in FIG. 5, and FIG. 7 is a cross- FIG. 8 is a schematic cross-sectional view for explaining the driving unit of FIG. 7, FIG. 9 is a perspective view showing a state where a tube is installed in the rotating body, FIG. 10 is a cross- 12 is a cross-sectional view corresponding to Fig. 10 showing another type of rotating body, Fig. 13 is a cross-sectional view taken along line AA of Fig. 12, Fig. The bottom of the rotating body And, Figure 14 is a an enlarged side view showing input, 15 is a close-up plan view showing a heating unit, Figure 16 is a structural diagram of a biological sample measuring device according to the invention.

2 to 4, the biological sample measurement apparatus 100 according to the present invention includes a body 110 having a space formed therein and having a first opening 1101, A lid 120 installed to cover the first opening 1101 of the main body 110 and an operation part 130 provided in a space formed inside the main body 110. The main body 110 includes a base plate 1107 for mounting the charging unit 1340 and the like. The charging unit 1340 and the like may be installed directly on the main body 110.

The cover 120 is rotatably fixed to the main body 110 by a hinge 1203. The cover 120 covers the first opening 1101 with the cover 120, It is preferable that the lid 120 includes an outer cover 1207 and an inner cover 1205 formed of an elastic member such as rubber to prevent light from entering between the lid 110 and the lid 120. The outer cover 1207 is located on the outer side of the main body 110 and the inner cover 1205 is positioned on the inner side of the main body 110 while the first cover 1101 is covered with the cover 120 So that external light can be prevented from entering between the main body 110 and the lid 120. It is preferable that a shielding film (not shown) is also provided on the inside of the main body 110. In FIG. 2, reference numeral 1201 denotes a handle used for opening and closing the lid 120.

The operation unit 130 includes an installation unit 1310 in which a container containing a reagent (for example, a bayer) is fixedly installed, a pump unit 1320 that draws a predetermined amount of the reagent contained in the container and supplies the reagent to the input unit 1340, A charging unit 1340 connected to the pump unit 1320 to administer the reagent supplied by the pump unit 1320 to the tube 2001 installed in the rotating body 1380, And a tube mounting portion provided with the tube. The first sensor 1355 may be disposed adjacent to the tube mounting portion. In one embodiment, the tube mounting portion includes a rotating body 1380 rotatably installed to the lower portion of the charging portion 1340, a first sensor 1355 fixed to the side of the rotating body 1380, And a driving unit 1370 for rotating the rotating body 1380. The container 1000 and the pump unit 1320 accommodated in the installation unit 1310 are connected to each other by a connection pipe 1327. The pump unit 1320 and the input unit 1340 are also connected to the connection pipe 1327, Lt; / RTI > The connection pipe 1327 is made of a polymer material and serves as a passage through which the reagent moves through the hollow tube.

The mounting portion 1310 includes a lower plate 1311 fixed to the main body 110 as shown in FIGS. 4, 5 and 6, a vertical plate 1311 extending upward from the lower plate 1311, And a plurality of upper plates 1313 extending from the vertical plate 1312 in parallel with the lower plate 1311. The upper plates 1313 are spaced apart from each other. The upper plate 1313 is fixed to the main body 110 by a plurality of fixing screws 1317 passing through the upper plate 1313 and the lower plate 1311. Both ends of the elastic body 1318 are fixedly connected to the two fixing screws 1317 of the fixing screws 1317. A plurality of recesses 1314 are formed in the upper plate 1313 between the fixing screws 1317 to which both ends of the elastic body 1318 are fixed. The container 1000 is located in the concave portion 1314 and is fixed by an elastic body 1318. The main body 110 may further include a base portion 1105 and the mounting portion 1310 may be fixed to the base portion 1105.

 And a second sensor portion 1330 composed of a plurality of sensors 1331, 1332, 1333, and 1334 that are installed on the vertical plate 1312 so as to be positioned between the upper plates 1313 spaced up and down Do. Each of the sensors 1331, 1332, 1333 and 1334 of the second sensor unit 1330 is installed in the recess 1314 and detects the presence or absence of the remaining amount of the reagent contained in the container installed in the recess 1314 .

The pump unit 1320 functions to draw reagents contained in the respective containers 1000 and supply the reagents to the input unit 1340. The pumps 1321 and 1322 connected to the respective containers 1000 by a connection pipe 1327 , 1323, 1324). The number of the pumps is not limited to four but may be adjusted according to the number of the vessels 1000.

Referring to FIG. 4 showing an example of a biological sample measurement apparatus 100 according to the present invention, four containers 1000 are installed in a mounting portion 1310 of a measurement apparatus 100 according to the present invention . 4, the reagents contained in the first container and the second container are pumped out to the third container, mixed in the third container, and then the third container and the fourth contained reagent are pumped out, To the tube provided in the rotating body 1380 so that the reaction takes place in the tube. Of course, it is also possible to install only two vessels in the above, withdraw reagents from the two vessels and supply them to the tubes.

The injecting part 1340 is provided at one side of the mounting part 1310 and supplies the reagent drawn out from the container 1000 to the tube 2001 installed in the rotating body 1380 through the pump part 1320 . The input unit 1340 includes at least one guide rod 1343 fixed to the base plate 1107 and a bracket 1341 provided at an end of the guide rod 1343 as shown in FIGS. A screw 1345 which is rotatably installed on the bracket 1341 on the one side and the base plate 1107 on the other side and a sensor rod 1348 fixed to the base plate 1107, A second motor 1353 that rotates the screw 1345 and a second motor 1353 that moves up and down along the guide rod 1343 by the rotation of the screw 1345, And an attachment member 1347 having an attachment surface 1347a.

The second motor 1353 is fixed to a lower portion of the base plate 1107 and connected to a screw 1345 protruding downward through a base plate 1107. The second motor 1353 may be connected to the screw 1345 through a speed reducer 1351.

The upper and lower sensor units include an upper sensor 1342 and a first tube sensor 1344 that are in contact with the mounting member 1347 and are spaced apart from the upper portion of the sensor bar 1348. In addition, And a second tube sensor 1346 installed at a lower portion of the first tube sensor 1344. 14, reference numeral 1349 denotes a fixing screw for fixing a nozzle to the mounting member 1347. A through hole is formed in the mounting member 1347 so that a nozzle is vertically formed, It is possible to fix the nozzle by inserting the fixing screw 1349 in the direction perpendicular to the through-hole forming direction.

As shown in FIGS. 4, 8 and 9 to 13, the rotating body 1380 includes a flange 1381 having a predetermined thickness and formed with an insertion hole 1382 so as to have a predetermined interval in the circumferential direction, A body 1383 formed at the lower portion of the flange 1381 and having a plurality of concave recesses 1384 connected to the outer surface thereof by the insertion holes 1382 and having a rotation shaft hole 1385 formed at the center thereof, And a plurality of rotary terminals 1387 spaced apart in the circumferential direction to the lower portion of the rotary shaft 1383. The flange 1381 is formed in a circular shape having a constant thickness and the body 1383 is formed in a circular shape having an outer diameter smaller than the outer diameter of the flange 1381.

As shown in FIGS. 10 to 13, it is preferable that the side surface of the mounting groove 1384 is formed as a mirror surface 1386 (mirror surface). In the case where the side surface of the mounting groove 1384 is formed as a mirror surface 1386 (mirror surface), light that is diverging from the tube 2001 and directed to the side surface is reflected by the mirror surface 1386 and reaches the first sensor 1355 , The amount of light transmitted to the first sensor 1355 increases, so that it is possible to more accurately measure the concentration by detecting minute divergence.

A plurality of insertion holes 1382 are formed in the flange 1381 at predetermined intervals in the circumferential direction and the insertion holes 1382 are extended to the main body 1383 so that a plurality of It is possible to form the groove 1384.

8, when the bottomed cylindrical tube 2001 is inserted into the insertion hole 1382, the tube 2001 is fixedly supported by the insertion hole 1382, 1384 and is exposed in the outer diameter direction of the main body 1383. The rotating body may be formed in a shape as shown in Figs. 11 and 13, so that different kinds of tubes can be inserted.

In Figs. 11 and 13, reference numeral 1389 denotes an identification terminal. The rotating body 1380 may be made of a polymer material.

7 and 8, the driving unit 1370 that rotates the rotating body 1380 includes a rotating shaft 1371 inserted into the rotating shaft hole 1385 of the rotating body 1380, A housing 1373 fixed to the rotary shaft 1371 and rotatably supporting the rotary shaft 1371 via a bearing 1375, a first motor 1379 connected to the rotary shaft 1371 to rotate the rotary shaft 1371, And a third drive sensor 1376 acting on the rotation terminal 1387. [ The third drive sensor 1376 is disposed at a position having a radius equal to the rotation terminal 1387 with respect to the rotation center of the rotation shaft 1371. When the rotation body 1380 rotates, And is positioned below the rotation terminal 1387.

The driving unit 1370 may further include a second driving sensor 1374 and a first driving sensor 1372 that operate in conjunction with the third driving sensor 1376 and the identification terminal 1389. The second drive sensor 1374 and the first drive sensor 1372 are installed at positions having the same radius as the identification terminal 1389 with respect to the rotation center of the rotation axis 1371 so that the rotation body 1380 rotates about the rotation axis 1371 It is positioned below the identification terminal 1389 and recognizes the rotating body 1380 and recognizes the shape of the rotating body 1380. [ Therefore, the position of the identification terminal 1389 can be recognized according to the tube inserted into the rotating body 1380. In other words, the rotating body 1380 attached to and detached from the driving unit 1370 can have various shapes, and the rotating bodies 1380 having various shapes can be distinguished from each other through the identification terminal 1389. The first driving sensor 1372 or the second driving sensor 1374 may detect the identification terminal 1389 and distinguish the rotating body 1380. The second tube sensor 1346 senses the lowering of the attachment member 1347 and allows the reagent to be injected into the tube through the nozzle at a predetermined position corresponding to the shape of the rotating body 1380.

4 and 7, reference numeral 1360 denotes a guide wall. The guide wall 1360 is fixed to the main body 110 of the base plate 1107, As shown in FIG. The guide wall 1360 is formed in a cylindrical shape and is open to one side and has sensor supporting walls 1361 at both ends thereof. The sensor supporting wall 1361 serves to support and fix the first sensor 1355 on both sides. A rotating body 1380 is installed inside the guide wall 1360 and a first sensor 1355 is installed between the sensor supporting walls 1361. 8, the first sensor 1355 is positioned at the side of the rotating body 1380 as shown in FIG. 8, and is positioned in the mounting groove 1384 and is exposed to the outer surface of the rotating body 1380 2001). ≪ / RTI >

The guide wall 1360 also acts to once again block external light from reaching the tube.

The biological sample measurement apparatus 100 according to the present invention may be configured such that the tube installed in the rotating body 1380 is heated to raise the temperature to a predetermined temperature and then installed in the rotating body 1380, (180).

2, 3 and 15, the heating means 180 includes a guide rail 1802 provided on both sides of the inside of the second opening formed at one side of the main body 110, A tray 1801 movably installed in the second opening along the second opening 1802, and a heating unit 1804 installed inside the second opening. The plate (2003) may be disposed on the tray (1801). The heating unit 1804 may be formed of a heat line installed at a lower portion or an upper portion of the tray 1801 at a position where the tray 1801 is inserted into the second opening as shown in FIG. A tube is disposed in the plate 2003, and the reaction material accommodated in the tube can be heated to a predetermined temperature by the heating unit 1804. The predetermined temperature may be a temperature required for the reactant to react with the reagent to emit light. In order to automatically move the tray 1801 to the inside or outside of the second opening, a screw 1809 is rotatably installed inside the second opening of the main body 110 by a bearing 1807, And the moving body 1811 fixed to the tray 1801 by moving the screw 1809 to the third motor 1808 and rotating the screw 1809 to the left and right in FIG. The tray 1801 is moved to the left and right in FIG. In order to control the rotation of the third motor 1808 by sensing the position of the tray 1801, a front sensor 1803 and an end sensor 1803 are disposed inside the second opening of the main body 110, (1805). In FIG. 15, reference numeral 1806 denotes a target which is provided in the tray 1801 and works with the front sensor 1803 and the end sensor 1805.

Hereinafter, the operation of the biological sample measurement apparatus 100 according to the present invention will be described with reference to FIG.

The biological sample measurement apparatus 100 according to the present invention is connected to a control unit 171 (microcomputer) as shown in FIG. 16 and displays an input unit 179 such as a keyboard or a mouse while checking contents displayed by the display unit 173, It is possible to install the operation command by inputting the operation command.

The control unit 171 is connected to the second sensor unit 1330, the first sensor 1355, the first motor 1379 and the second motor 1353 of the biological sample measurement apparatus 100 according to the present invention, The third motor 1808 and the upper and lower sensor units 1342, 1344 and 1346, the pump unit 1320, the heating unit 1804, the front sensor 1803 and the end sensor 1805, And transmits the sensed signal to the control unit 171, and operates according to a control command of the control unit 171. [

In one embodiment, the second antibody 25 to which the enzyme 27 is bound, shown in FIG. 1, is referred to as a tube 2001 (hereinafter, referred to as 'enzyme tube 2001') coupled with the antigen 23 .) Is installed on the plate 2003. Fig. In other words, as described above with reference to FIG. 1, an antigen 23 acting on the first antibody 21 is introduced into the tube 10 to which the first antibody 21 is attached, and the first antibody 21 21). ≪ / RTI > The enzyme 27 is conjugated with the second antibody 25 acting on the antigen 23. The second antibody 25 acts on the antigen 23 bound to the first antibody 21 and binds thereto. Then, the second antibody (25) which is not bound to the antigen (23) is washed and removed. The enzyme tube 2001 in which the second antibody 25 conjugated with the enzyme 27 is bound to the antigen 23 can be prepared. In this case, the antigen 23 coupled with the second antibody 25 to which the enzyme 27 prepared in the enzyme tube 2001 is bound acts as a reactant and a biological sample. The control unit 171 controls the display unit 173 so that the control unit 171 controls the display unit 173 so that the control unit 171 controls the display unit 173, 3 motor 1808 to cause the tray 1801 to move into the second opening along the guide 1802 so that when the tray 1801 is moved into the second opening the target 1806 is positioned in front of the end sensor 1805 The end sensor 1805 operates to transmit a detection signal to the control unit 171 and the control unit 171 stops the operation of the third motor 1808 according to the detection signal of the end sensor 1805.

When the operation of the third motor 1808 is stopped, power is supplied to the heating unit 1804 to raise the temperature of the tube 2002 located in the tray 1801. The second opening may further include a thermometer to shut off the power supplied to the heating unit 1804 when the temperature reaches a predetermined temperature while measuring the temperature. The power to the heating unit 1804 is cut off and the third motor 1808 is operated so that the tray 1801 slides outside the second opening in a state as shown in Fig. At this time, when the target 1806 is positioned in front of the front sensor 1803, the operation of the third motor 1808 is stopped by the operation signal of the front sensor 1803. The front sensor 1803 and the end sensor 1805 can also act as an on / off switch to cut off or connect the power supplied directly to the third motor 1808.

When the enzyme tube 2001 is inserted into the through hole 1382 of the rotating body 1380 disposed inside the guide wall 1360, the lower portion of the enzyme tube 2001 is located in the attachment groove 1384, Exposed to the outer surface.

The first driving sensor 1372 or the second driving sensor 1374 senses the identification terminal 1389 provided under the rotating body 1380 and transmits a signal to the controller 171 so that the rotating body 1380 Can be adjusted by the rotation change shown in Figs. 8 to 11, the rotation change shown in Figs. 12 and 13, and the lowering height of the insertion portion 1340 can be adjusted.

The enzyme tube 2001 is inserted into the rotating body 1380 and the lid 120 is closed to block the entrance of external light and then an analysis command is inputted through the input unit 179. Then, The first pump 1321 and the second pump 1322 are operated to pump the reagents contained in the first and second containers from the left side of FIG. 4 into the third container to be mixed in the third container. The third pump 1323 pumps the reagent, and the fourth pump 1324 pumps the reagent contained in the fourth container and supplies it to the charging unit 1340.

When the screw 1345 is rotated by the operation of the second motor 1353 of the charging unit 1340, the mounting member 1347 is lowered and the first tube sensor 1344 or the second tube sensor 1346 is installed The second motor 1353 is stopped and the third pump 1323 and the fourth pump 1324 are operated so that the mixing reagent contained in the third container and the reagent contained in the fourth container The third pump 1323 and the fourth pump 1324 are supplied to the enzyme tube 2001 through the nozzles of the injection unit 1340. When the operation of the third pump 1323 and the fourth pump 1324 is stopped, The mounting member 1347 rises until it is sensed by the upper sensor 1342. [

The reagent contained in the first container is Amplex Red (10-acetyl-3,7-dihydroxyphenoxazine), which is an electron donor that reacts with hydrogen peroxide water (H2O2) by the presence of enzyme (HRP, Horseradish Peroxidase) And the reagent contained in the second container may be 1,1'-Oxalyldiimidazole (ODI), which is a luminescent material that reacts with the fluorescent material to emit light. And the reagent contained in the fourth vessel may be hydrogen peroxide (H2O2).

When the reagent is injected as described above, the reagent reacts due to the presence of the enzyme 27, and light is emitted. The emitted light is detected by the first sensor 1355, which is an optical sensor, converted into a digital signal, 171 and stored in the storage unit 177 while being calculated as the concentration of the antigen 23 in comparison with the data previously input to the storage unit 177. [

When the mounting member 1347 rises, the first motor 1379 operates to rotate the rotating body 1380, and the third driving sensor 1376 rotates to the rotating terminal 1387 provided below the rotating body 1380, The first motor 1379 stops. The rotating body 1380 is also stopped and the adjacent enzyme tube 2001 is positioned below the charging unit 1340 and the charging unit 1340 is lowered so that the reagent And the emitted light of the reagent is sensed by the first sensor 1355 and the detected light amount is converted into a digital signal and is calculated by the arithmetic unit 175 in terms of the concentration and stored in the storage unit 177. The process of calculating the operation and concentration of the charging unit 1340 and the pump unit 1320 is the same as described above, and therefore, the repetitive description will be omitted.

The first driving sensor 1372 and the second driving sensor 1374 are used to discriminate the rotating body 1380. When it is necessary to vary the input height according to the rotating body 1380, The first tube sensor 1344 may include a second tube sensor 1346 in addition to the first tube sensor 1344 to detect the identification terminal 1389 in the first drive sensor 1372, The rotation of the second motor 1353 is stopped when the member 1347 is detected and the rotation of the second motor 1353 is detected by the second tube sensor 1346 when the second driving sensor 1374 detects the identification terminal 1389. [ The rotation of the second motor 1353 is stopped to adjust the input height of the input unit 1340. [

In another embodiment, the biological sample measurement apparatus 100 may measure a biological sample by measuring chemiluminescence generated during the reaction of the target substance and the aptamer. More specifically, it is as follows. In the tube 2001, at least one selected from the group consisting of phenylglyoxal, phenylglyoxal derivative, and combinations thereof may be contained in the tube 2001 as a reaction material. For example, as the phenylglyoxal derivative, acetyl, oxy, methoxy, C1-C6 linear alkyl or C1-C6 branched alkyl may be bonded to a phenyl ring as a substituent. As another example, the phenylglyoxal derivative may be at least one selected from the group consisting of phenylglyoxal, 3-methoxyphenylglyoxal, 4-methoxyphenylglyoxal, 3,4-dimethoxyphenylglyoxal, 3,5-dimethoxyphenylglyoxal, 3,4,5-trimethoxyphenylglyoxal Either one may be used. For convenience of explanation, TMPG will be mainly described as the reaction material accommodated in the tube 2001.

In the first container, a secretion such as saliva, blood or urine of a person having a biological sample as a reagent may be quantitatively injected. The biological sample may be, for example, a protein, a microorganism, a virus, an organic substance, an inorganic substance, a chemical substance, or the like. The inorganic material may be, for example, sodium (Na) or potassium (K) present in the urine.

The second container may contain a reagent containing guanine. In this case, the reagent contained in the second container may be a DNA or RNA sequence containing guanine. The DNA or RNA sequence containing the guanine may be an aptamer capable of binding with the biological sample. Aptamers are ligands of interest, i.e., oligonucleotides designed to bind to the biological sample. That is, the aptamer can be selected as appropriate to bind with the selected biological sample. Fluorescent labels can be attached to the DNA or RNA sequences. By a chemiluminescent resonance energy transfer (CRET) generated between the energy intermediate generated in the course of the reaction between guanine and phenylglyoxal or a guanine and a phenylglyoxal derivative and the fluorescent label bound to the DNA or RNA sequence, The intensity of light generated during the reaction between the liquid material 10 and the second liquid material 20, the pattern of the emitted light, and the lifetime of the light can be changed. It is possible to adjust the intensity of the light, the pattern of the emitted light, the lifetime of the light, etc., which are generated through the selection of the fluorescent markers. These fluorescent labels include, for example, pacific blue, fluorescein, 6-FAM, Cy3, Cy3.5, Cy5, Cy5.5, HEX, TET, VIC, NED, JOE, ROX, Texas Red, Phodamine Green, Rhodamine Red, And a combination of these may be used.

The fourth container contains at least one selected from tetra-n-methyl ammonium phosphate, tetra-n-ethyl ammonium phosphate, tetra-n-propyl ammonium phosphate (TPA) Reagent may be contained. For convenience of explanation, the TPA will be mainly described as the reagent contained in the fourth container.

The biological sample contained in the first container is mixed with the reagent contained in the second container, i.e., the aptamer, in the third container. After the mixing, the mixed solution of the biological sample and the aptamer contained in the third container is injected into the tube 2001 together with the TPA contained in the fourth container and reacted with the reaction material contained in the tube 2001, that is, TMPG, to chemiluminesce. That is, the guanine contained in the aptamer reacts with the TMPG contained in the tube 2001 to chemiluminesce. At this time, the TPA functions as a catalyst for changing the reaction rate or the reaction pattern of chemiluminescence. In this case, the degree of chemiluminescence varies depending on how much the biological sample and the aptamer react with each other, and the emitted light is measured by the first sensor 1355, which is a photosensor. Therefore, by measuring the degree of chemiluminescence, the concentration of the biological sample contained in the first container can be measured. Since the operation of the remaining biological sample measurement apparatus 100 is sufficiently inferred from the above description, a detailed description thereof will be omitted for convenience of explanation. Accordingly, the biological sample measurement apparatus 100 disclosed in this specification can measure the intensity of light, the pattern of light, the lifetime of light, etc., which are generated differently depending on the presence or absence of the biological sample, Can be diagnosed.

According to the biological sample measurement apparatus 100 of the present invention as described above, it is possible to accurately inject the light emitting reagent into each tube while rotating the sample tube 1380 with a plurality of tubes at a predetermined angle, It is possible to rapidly measure the luminescence and to inject the reagent into the tube precisely since the injecting part 1340 moves upward and downward when the emitting reagent is injected, Since the side surface of the mounting groove 1384 of the body 1381 of the rotating body 1380 is formed as the mirror surface 1386, it is possible to accurately detect the small light emission, thereby improving the accuracy of analysis. The temperature of the tube can be adjusted, and the temperature of the tube can be increased by providing the heating means 180, The reagent container can be fixed without being shaken while the structure of the mounting portion 1310 is simplified.

Although the present invention has been shown and described with respect to specific embodiments thereof, it will be understood by those skilled in the art that various changes, modifications, and variations can be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Anyone with the knowledge of this will know easily. And that the various embodiments disclosed are not to be construed as limiting the scope of the disclosed subject matter, but true ideas and scope will be set forth in the following claims.

100: Biological sample measuring device
110:
120: Cover
130:
1310: Installation part
1320:
1330: second sensor unit
1340:
1355: first sensor
1370:
1380: rotating body
180: heating means

Claims (14)

A measuring device used for measuring a biological sample,
The measuring device
A body portion having a first opening formed therein with a space inside;
A lid for opening and closing the first opening of the main body; And
And an actuating part installed in the space provided in the main body part,
The operating portion
An installation part in which a reagent vessel is installed;
A pump unit provided at one side of the mounting unit to supply a reagent contained in the reagent container to the outside;
An injection unit for injecting the reagent supplied by the pump unit into the tube;
A tube mounting portion provided at a lower portion of the charging portion and having the tube installed therein; And
And a first sensor disposed adjacent to the tube mounting portion,
The tube contains a reactant,
The reaction material is mixed with the reagent supplied from the charging unit in the tube,
Wherein the first sensor measures the concentration of the biological sample contained in the reagent or the reactant by measuring light emission generated by the mixing of the reagent and the reactive material. The method according to claim 1,
The tube mounting portion
A rotating body provided at the lower portion of the charging unit; And
And a driving unit for rotating the rotating body,
The rotating body
A flange spaced apart in the circumferential direction and having a plurality of insertion holes; And
And a body provided with a plurality of concave installation grooves formed on an outer surface of the flange and connected to the insertion hole, the body having a rotation shaft hole formed in a central portion thereof,
Wherein the driving unit is connected to the rotating body through the rotating shaft,
The tube is fixedly inserted into the insertion hole,
Wherein the first sensor is disposed laterally of the rotating body to measure the light emission generated by the mixing of the reagent and the reactive material in the tube. 3. The method of claim 2,
Wherein the body portion includes a base plate,
Wherein the rotating body includes a plurality of rotating terminals spaced circumferentially at a lower portion of the main body,
The driving unit
A rotating shaft inserted into the rotating shaft;
A housing fixed to the base plate and rotatably supporting the rotation shaft;
A first motor connected to the rotation shaft; And
And a third drive sensor acting on the rotary terminal,
The tube is composed of a plurality of tubes,
A plurality of the tubes are respectively disposed in the plurality of insertion holes,
Wherein the rotary terminal is provided at the lower portion of the main body of the rotating body so as to correspond to the plurality of insertion holes,
Wherein the first motor rotates the rotating body, stops rotating when the third driving sensor senses the rotating terminal,
Wherein the injecting part injects the reagent into a tube disposed opposite to the first sensor among a plurality of the tubes after the rotation of the rotating body stops,
Wherein the first sensor measures the light emission generated by the mixing of the reagent and the reactant in the tube disposed opposite the first sensor among the plurality of tubes,
And the first motor rotates the rotating body when the light emission measurement by the first sensor is completed. 4. The method according to any one of claims 1 to 3,
A plurality of reagent containers are installed in the mounting portion,
The plurality of reagent vessels include a first reagent vessel, a second reagent vessel, and a third reagent vessel,
The pump unit withdraws the reagent contained in the first reagent vessel and the reagent contained in the second reagent vessel and supplies the reagent to the third reagent vessel to produce a mixed reagent, And supplies the sample to the outside. 5. The method of claim 4,
Wherein the plurality of reagent vessels comprise a fourth reagent vessel,
The pump unit withdraws the reagent contained in the first reagent vessel and the reagent contained in the second reagent vessel and supplies the reagent to the third reagent vessel to produce a mixed reagent, And the reagent contained in the fourth reagent vessel is withdrawn and supplied to the outside. 6. The method of claim 5,
Wherein the reactant comprises an antigen conjugated with an enzyme and a second antibody, the antigen being associated with a first antibody attached to the tube,
The reagent contained in the first reagent vessel and the reagent contained in the second reagent vessel and the other reagent respectively react with an aqueous solution of hydrogen peroxide by the presence of the enzyme to form an electron donor And a luminescent material that reacts with the fluorescent material to emit light,
Wherein the reagent contained in the fourth reagent vessel contains the hydrogen peroxide,
Wherein the light is emitted through the mixture of the reactant, the reagent, the reagent, and the hydrogen peroxide in the tube. The method according to claim 6,
Wherein the enzyme is HRP (Horseradish peroxidase), the electron donor is Amplex-Red (10-acetyl-3,7-dihydroxyphenoxazine), and the luminescent material is 1,1'-Oxalyldiimidazole. 6. The method of claim 5,
Wherein the reactive material comprises at least one selected from the group consisting of phenylglyoxal, phenylglyoxal derivative, and combinations thereof,
Wherein the reagent contained in the first reagent vessel comprises any one selected from the group consisting of saliva, blood, urine, and combinations thereof, including sodium (Na) or potassium (K)
The reagent contained in the second reagent vessel reacts with the sodium or potassium and is an aptamer having a DNA or RNA sequence containing guanine,
The reagent contained in the fourth reagent vessel may be tetra-n-methyl ammonium phosphate, tetra-n-ethyl ammonium phosphate, tetra-n-propyl ammonium phosphate (TPA) And at least one selected from the plurality of biological samples. The method of claim 3,
The input unit
At least one guide rod fixedly installed on the base plate;
A sensor rod fixedly installed on the base plate;
A bracket fixed to an upper portion of the guide rod;
A screw rotatably installed on the bracket and the base plate;
An installation member moving up and down along the guide rod by rotation of the screw;
An upper sensor and a first tube sensor installed in the sensor bar;
A nozzle provided on the mounting member; And
And a second motor connected to a lower portion of the screw to rotate the screw,
The reagent is injected into the tube through the nozzle,
Wherein the upper sensor senses the rising of the attachment member and the first tube sensor senses the lowering of the attachment member so that the reagent is injected into the tube through the nozzle at a predetermined position. The method of claim 3,
Wherein the side surfaces of the plurality of mounting grooves formed concavely in the outer surface of the main body are formed in a mirror surface so as to form light in the lateral direction of the plurality of mounting grooves among light generated by the mixing of the reagent and the reactive material Is reflected on the specular surface to be directed to the first sensor. 10. The method of claim 9,
Wherein the rotating body is detachably disposed in the driving unit,
Wherein the rotating body has an identification terminal at a lower portion of the main body,
The driving unit may further include a first driving sensor or a second driving sensor;
Wherein the charging unit further comprises a second tube sensor provided below the first tube sensor in the sensor rod,
The rotating body, which is detachably attached to the driving unit, may have various shapes, and the rotating bodies having various shapes may be separated from each other through the identification terminal,
The first drive sensor or the second drive sensor senses the identification terminal to distinguish the rotating body,
Wherein the second tube sensor senses the lowering of the attachment member and allows the reagent to be injected into the tube through the nozzle at a predetermined position corresponding to the shape of the rotator. The method of claim 3,
Wherein the measuring device further comprises a heating means,
The heating means
A guide rail provided at both sides of a second opening formed at one side of the main body;
A tray disposed on the tray and movable along the guide rail through the second opening; And
And a heating portion provided inside the second opening,
The tube is disposed on the plate,
Wherein the reaction material accommodated in the tube is heated to a predetermined temperature by the heating unit. The method of claim 3,
The mounting portion
A lower plate fixed to the body portion;
A vertical plate extending upwardly from said bottom plate;
A plurality of upper plates extending from the vertical plate in parallel with the lower plate;
A plurality of fixing screws passing through the lower plate and the upper plate to fix the upper plate to the main body; And
And an elastic body having both ends fixed to the fixing screw,
A plurality of recesses are formed in an upper plate between the fixing screws to which the elastic body is fixed,
Wherein the reagent vessel is located in the recess and is fixed by the elastic body. 14. The method of claim 13,
Further comprising a second sensor disposed on the vertical plate for detecting whether the reagent is contained in the reagent container.

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