KR20160126272A - Polymer for evaluation method of biodegradation and evaluation method of biodegradation using the same - Google Patents
Polymer for evaluation method of biodegradation and evaluation method of biodegradation using the same Download PDFInfo
- Publication number
- KR20160126272A KR20160126272A KR1020150057146A KR20150057146A KR20160126272A KR 20160126272 A KR20160126272 A KR 20160126272A KR 1020150057146 A KR1020150057146 A KR 1020150057146A KR 20150057146 A KR20150057146 A KR 20150057146A KR 20160126272 A KR20160126272 A KR 20160126272A
- Authority
- KR
- South Korea
- Prior art keywords
- standard specimen
- biodegradability
- biodegradable polymer
- specimen
- evaluation method
- Prior art date
Links
- 238000011156 evaluation Methods 0.000 title claims abstract description 36
- 238000006065 biodegradation reaction Methods 0.000 title claims abstract description 26
- 229920000642 polymer Polymers 0.000 title abstract description 8
- 229920002988 biodegradable polymer Polymers 0.000 claims abstract description 42
- 239000004621 biodegradable polymer Substances 0.000 claims abstract description 42
- 238000000034 method Methods 0.000 claims abstract description 26
- 244000005700 microbiome Species 0.000 claims abstract description 26
- 239000002689 soil Substances 0.000 claims abstract description 17
- 230000008859 change Effects 0.000 claims abstract description 14
- 238000005227 gel permeation chromatography Methods 0.000 claims abstract description 13
- 238000004458 analytical method Methods 0.000 claims abstract description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 14
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 14
- 239000001569 carbon dioxide Substances 0.000 claims description 7
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 7
- 239000007789 gas Substances 0.000 abstract description 37
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 abstract description 4
- 238000010998 test method Methods 0.000 description 16
- 231100000209 biodegradability test Toxicity 0.000 description 12
- 239000010802 sludge Substances 0.000 description 11
- 239000007853 buffer solution Substances 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 9
- 229920003023 plastic Polymers 0.000 description 7
- 239000004033 plastic Substances 0.000 description 7
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000002699 waste material Substances 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
- 239000004626 polylactic acid Substances 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 229920006167 biodegradable resin Polymers 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 238000003912 environmental pollution Methods 0.000 description 4
- 238000009629 microbiological culture Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000010451 perlite Substances 0.000 description 4
- 235000019362 perlite Nutrition 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000010455 vermiculite Substances 0.000 description 4
- 229910052902 vermiculite Inorganic materials 0.000 description 4
- 235000019354 vermiculite Nutrition 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000003415 peat Substances 0.000 description 3
- 239000001103 potassium chloride Substances 0.000 description 3
- 235000011164 potassium chloride Nutrition 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000000538 analytical sample Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- QDHHCQZDFGDHMP-UHFFFAOYSA-N Chloramine Chemical compound ClN QDHHCQZDFGDHMP-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 150000002013 dioxins Chemical class 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- -1 peatmoss Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- TWHXWYVOWJCXSI-UHFFFAOYSA-N phosphoric acid;hydrate Chemical compound O.OP(O)(O)=O TWHXWYVOWJCXSI-UHFFFAOYSA-N 0.000 description 1
- 239000000088 plastic resin Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000010801 sewage sludge Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000013585 weight reducing agent Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/44—Resins; Plastics; Rubber; Leather
- G01N33/442—Resins; Plastics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
- G01N33/0036—General constructional details of gas analysers, e.g. portable test equipment concerning the detector specially adapted to detect a particular component
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
- G01N33/0036—General constructional details of gas analysers, e.g. portable test equipment concerning the detector specially adapted to detect a particular component
- G01N33/004—CO or CO2
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Combustion & Propulsion (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to a polymer specimen for evaluating biodegradability and a method for evaluating biodegradability using the specimen. More particularly, the present invention relates to a standard specimen for standardization of various biodegradability evaluation methods, And a method for evaluating biodegradability using the same. BACKGROUND OF THE INVENTION 1. Field of the Invention [0002] The present invention relates to a biodegradability evaluation method,
In the standardization of the biodegradability evaluation method according to the present invention, the degree of biodegradation is evaluated by using a biodegradability evaluation method for measuring the change of the biodegradable polymer standard specimen, wherein the biodegradable polymer standard specimen includes the planar porosity . In addition, the size of the biodegradable polymer standard specimen is standardized.
The biodegradability evaluation method comprises measuring the gas generation rate of a gas generated after biodegrading the biodegradable polymer standard specimen with a microorganism, measuring the surface area of the biodegradable polymer standard specimen, and the biodegradability Calculate the rate of gas generation per unit area of the polymer standard specimen, and compare them to make a comparison.
In addition, the biodegradability evaluation method is characterized in that the biodegradable polymer standard specimen is placed on a soil, and the change in molecular weight is measured through gel permeation chromatography (GPC) analysis, and then indexed and compared.
In addition, the biodegradable polymer standard specimen is biodegraded in a solution having a different hydrogen ion concentration index (pH), and then the difference in weight change is measured, and then indexed and compared.
Description
The present invention relates to a polymer specimen for evaluating biodegradability and a method for evaluating biodegradability using the specimen. More particularly, the present invention relates to a standard specimen for standardization of various biodegradability evaluation methods, And a method for evaluating biodegradability using the same. BACKGROUND OF THE INVENTION 1. Field of the Invention [0002] The present invention relates to a biodegradability evaluation method,
Recently, environmental pollution has become a serious social problem both domestically and globally. Plastic materials have made a great contribution to the abundant daily life and industrial development, while various kinds of waste vinyl and waste plastics, which are released in large quantities according to the use of plastics, have become one of the main causes of environmental pollution. Environmental hormones generated by incineration or landfill of waste plastic and waste plastics, dioxins that are highly toxic, and air pollution caused by incomplete combustion of wastes cause serious environmental pollution.
Recently, since the problem of environmental pollution caused by the waste plastic has become a big problem in recent years, the environment-friendly biodegradable plastic resin, which is decomposed by microorganisms in the soil, have. Generally, "biodegradable" means the ability of a compound to be completely degraded into biological resources such as methane, carbon dioxide and water or inorganic salts by microorganisms and / or natural environmental factors. In order to protect the global environment, various international environmental conventions in various fields are being continuously implemented and implemented. In addition to the strengthening of environmental regulations, consumer awareness about chemical products and various consumer products has changed. Efforts are continuing. Already in developed countries such as Germany, Italy and the United States, it is obligatory to use biodegradable resins for shopping bags and plastic bottles.
In order to study and use biodegradable polymers, biodegradability measurement method and biodegradation criteria should be prioritized. However, in spite of the research on biodegradable polymers and the rapid increase of products made of biodegradable polymer compounds and the expansion of the market, a standardization work has been carried out to make the biodegradability of the biodegradable polymer compounds fast, quantitative and reproducible It is not.
The biodegradability of biodegradable polymer materials has been studied in the United States and Japan as a standardized method. However, in the United States, abandoned polymeric materials have a tendency to be biodegradable due to landfill There is a problem that it is difficult to evaluate the biodegradability quantitatively in a reproducible manner and there is a problem that the measurement time is very long. In addition, the biodegradation process based on the existing KSM 3100-1 is a biodegradation measurement method which is performed for at least 45 to 180 days at a temperature of 58 ° C and a humidity of 50%. In addition, the evaluation cost is very high, There is a limitation in research on efficient biodegradable polymers.
SUMMARY OF THE INVENTION The present invention has been made in order to solve the above problems, and it is an object of the present invention to select standard specimens to be standard for standardizing and comparing various biodegradability evaluation methods, and to carry out the test method accordingly.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not restrictive of the invention as set forth in the accompanying drawings. It will be possible.
In the standardization of the biodegradability evaluation method according to the present invention, the degree of biodegradation is evaluated by using a biodegradability evaluation method for measuring the change of the biodegradable polymer standard specimen, wherein the biodegradable polymer standard specimen includes the planar porosity . In addition, the size of the biodegradable polymer standard specimen is standardized.
The biodegradability evaluation method comprises measuring the gas generation rate of a gas generated after biodegrading the biodegradable polymer standard specimen with a microorganism, measuring the surface area of the biodegradable polymer standard specimen, and the biodegradability Calculate the rate of gas generation per unit area of the polymer standard specimen, and compare them to make a comparison.
In addition, the biodegradability evaluation method is characterized in that the biodegradable polymer standard specimen is placed on a soil, and the change in molecular weight is measured through gel permeation chromatography (GPC) analysis, and then indexed and compared.
In addition, the biodegradable polymer standard specimen is biodegraded in a solution having a different hydrogen ion concentration index (pH), and then the difference in weight change is measured, and then indexed and compared.
According to the solution of the above-mentioned problems, the standardization of the biodegradability test method of the present invention has the effect of standardizing a variety of biodegradability evaluation methods by producing reference standard specimens.
In addition, various biodegradability evaluation methods can be compared and standardized and analyzed.
1 is a perspective view showing a standard specimen produced according to the present invention;
Fig. 2 is a photograph showing the state of a standard specimen on a culture soil in a biodegradability test method using soil at a certain temperature
3 is a flowchart showing a test method of biodegradation using microorganisms using a standard specimen
The above and other objects, features and advantages of the present invention will be more apparent from the following detailed description taken in conjunction with the accompanying drawings, in which: FIG. BRIEF DESCRIPTION OF THE DRAWINGS The advantages and features of the present invention and the manner of achieving them will become apparent by reference to an embodiment which will be described in detail below with reference to the accompanying drawings.
The present invention relates to a method for evaluating biodegradability using standard specimens, more particularly, to select standard specimens for standardization of various biodegradability evaluation methods, And a method of evaluating biodegradability using a standard sample that standardizes the method so as to be comparable.
The standard specimen using the biodegradability evaluation method is prepared by including a flat surface porosity and its size being standardized. Specifically, the porosity is to maximize the surface area of the standard specimen, and the biodegradability evaluation reaction, which will be described later, is promoted through the maximized surface area, thereby increasing the efficiency of biodegradation measurement. Therefore, when the conventional biodegradability evaluation method was used, it took more than two years to evaluate the biodegradability of polylactic acid (PLA), which has the longest decomposition period of the biodegradable resin. However, The biodegradability test allows the evaluation method to be carried out with a period of time suitable for use in the actual industry.
Further, the standard specimen is characterized by being manufactured in a standardized flat surface. The standard specimen is provided in a standardized planar shape and it is easy to evaluate the degree of biodegradability.
Specifically, in the method for evaluating biodegradability by biodegradation as a microorganism among the biodegradability assessment methods of the present invention, the standard specimen is provided on a standardized flat surface, and it is easy to compare the surface area difference after biodegradation to facilitate indexing.
In the biodegradability evaluation method using the soil at a certain temperature among the biodegradability evaluation methods of the present invention, as shown in FIG. 2, the standard specimen is provided on a standardized flat surface, It is easy to measure the change of the molecular weight and to make the index.
In addition, a method for measuring a difference in weight change after biodegradation in a solution having a different hydrogen ion concentration index (pH) among the biodegradability assessment methods according to the present invention is characterized in that the standard specimen is provided on a standardized plane, And it is easy to measure the difference of weight change after biodegradation and to index it.
The volume and surface area of the standard specimen prepared in the present invention can be confirmed through FIG. 1 and the following examples.
[Comparative Example 1]
In Comparative Example 1, polylactic acid (PLA) having the longest degradation rate was used as biodegradable resin, and 3001D grade of naturewokrs Co. was used. When the specimen was prepared with the polylactic acid (PLA) weighing 8.370 g, the surface area of the specimen was measured as 5.190 cm < 2 >.
[Example 1]
In Example 1, the same polylactic acid (PLA) as that of Comparative Example 1 was used and a standardized flat standard specimen having porosity was prepared.
As shown in FIG. 1, the standard specimen has a size of 90
When manufactured to the above standard, the surface area of the standard specimen is measured as 6.064 cm < 2 >.
[Example 2]
In Example 2, the same polylactic acid (PLA) as that in Comparative Example 1 was used, and a standard specimen having a porous structure was prepared.
As shown in FIG. 1, the standard specimen has a size of 90
When manufactured to the above standard, the surface area of the standard specimen is measured to be 6.080 cm < 2 >.
The biodegradability of the biodegradable resin can be measured, compared, and indexed by the following biodegradability test method using the standard specimen prepared through the above examples.
(1) Biodegradability test method using soil at a certain temperature
The biodegradability test method using the soil at the above-described temperature is characterized in that the biodegradable polymer standard specimen is placed on a soil and then the change of the molecular weight is measured by gel permeation chromatography (GPC) analysis, and then indexed and compared .
First, the molecular weight of the standard specimen prepared according to the present invention is measured, and the standard specimen of the present invention is buried in the culture soil of the thermo-hygrostat according to the conditions of Table 2.
Concretely, in the constant temperature and humidity bath having the conditions of pH of 7.0 to 9.0, temperature of 56 to 60 ° C and water content of 40 to 60 wt%, the culture soil was at least one of bark, perlite, peat moss and vermiculite ≪ / RTI >
In addition, the biodegradability test method using the soil is performed by checking the degree of biodegradation at room temperature, so that the reproducibility of the test method is obtained. As shown in Table 2, at the room temperature condition, the pH was 7.0 to 9.0, the temperature was 23 to 27 ° C, and the moisture content was 40 to 60 wt%. In the constant temperature and humidity bath, the culture soil was bark, perlite, peatmoss, vermiculite And at least one of the components.
Next, the standard specimen of the present invention is arranged as shown in Fig. Specifically, seven standard specimens are placed on the culture soil of the constant temperature and humidity bath to prepare experimental conditions.
Next, the standard specimen on the culture soil of the constant temperature and humidity bath is recovered and the molecular weight is measured by gel permeation chromatography (GPC) analysis. In the present invention, the biodegradability is standardized by measuring the molecular weight of the above-mentioned standard specimen by the Korea Polymer Research Institute. The gel permeation chromatography (GPC) analysis environment is shown in Table 3 below.
(2) Biodegradability test method using hydrolysis promotion
The biodegradability test method using the hydrolysis promotion is characterized in that the biodegradable polymer standard specimen is biodegraded in a solution having a different hydrogen ion concentration index (pH), then the difference in weight change is measured, and the biodegradable polymer standard specimen is indexed and compared .
First, the weight of a standard specimen prepared according to the present invention is measured, and a buffer solution is prepared according to the conditions shown in Table 4.
As shown in Table 4, the buffer solution of pH 2 is prepared by mixing 75.6 g of calcium phosphate (KH 2 PO 4 ) and 60 mL of phosphoric acid (H 3 PO 4 ). The buffer solution of pH 7 is prepared by mixing 6.6 g of potassium phosphate (KH 2 PO 4) and 30 g of phosphate hydrate (NaHPO 4 .12H 2 O). The buffer solution of pH 12 is prepared by mixing 192 g of phosphate (NaHPO 4 ) and 18.35 g of sodium chloride (NaOH).
Next, the buffer solution of pH 2, pH 7, and pH 12 is dissolved in distilled water to prepare a 1 L solution, and then the standard specimen prepared according to the present invention is placed in the solution. Specifically, the dissolved buffer solution is maintained at 37 ° C. and two buffer solutions of pH 2, pH 7, and pH 12 are prepared in order to improve the accuracy and reproducibility of the experiment.
Next, the standard specimens reacted in the buffer solution are taken out at intervals of 7 days, weighted and measured for reduction, and standardized. The result of the weight reduction amount takes an average value.
(3) Biodegradability test method using microorganisms
As shown in FIG. 3, the biodegradability test method using the microorganism is a method of measuring the rate of gas evolution of the biodegradable polymer standard specimen after biodegradation of the biodegradable polymer standard specimen into microorganisms, measuring the surface area of the biodegradable polymer standard specimen The gas generation rate per unit area of the biodegradable polymer standard specimen is calculated using the gas generation rate, and then indexed for comparison.
In addition, it is preferable that the gas generation rate is a gas generation rate of carbon dioxide (CO 2 ) under an aerobic condition and a gas generation rate of methane (CH 4 ) in an anaerobic condition.
Specifically, the gas generation rate per unit area of the reference sample, which is indexed in the biodegradation evaluation method using the microorganism, is defined by the following equation (1).
Hereinafter, a method for evaluating biodegradability using the microorganisms will be described in detail with reference to the drawings.
3 is a flowchart showing a flow of a method for evaluating biodegradability using microorganisms according to the present invention.
First, in a first step (S10), the standard specimen is introduced into a closed flask, and a culture medium for culture and an anaerobic sludge are introduced into the closed flask.
Specifically, 0.1 parts by weight of the anaerobic sludge was added to 1 part by weight of the microbial culture medium prepared according to the conditions of Table 5, after putting the standard specimen into a 500 mL closed flask.
As shown in Table 5, the microorganism culture medium was prepared by adding chloramine (NH 2 Cl), sodium monophosphate (NaH 2 PO 4 .H 2 O), sodium hydrogen phosphate (Na 2 HPO 4 ), potassium chloride KCl), vitamins and minerals, and the acetate is added to the initial microorganism growth substrate and the pH is titrated to neutral (pH 7.0) suitable for microbial growth.
The chemical composition of the microorganism culture medium can be adjusted depending on the inoculum, and the pH can be adjusted to a pH of 6.5 to 7.5 suitable for general microbial growth and metabolism.
In addition, the anaerobic sludge uses sludge that has been filtered by removing impurities from sewage sludge. The anaerobic sludge is used as a microorganism inoculation source containing various microorganisms at a high concentration and can be replaced with a mixed microorganism including pure microorganisms or anaerobic sludge. In the present invention, it was collected after being harvested from the sewage treatment plant of Haeundae-gu.
The amount of the microorganism culture medium and anaerobic sludge is adjusted according to the amount of the analytical sample.
Next, in the second step S20, the flask is closed and incubated in a thermostatic stirrer. Specifically, the standard specimen, the microorganism culture medium, and the flask containing the anaerobic sludge are sealed and fixed by a constant temperature stirrer.
The microorganisms of the microorganism culture medium and the anaerobic sludge are adhered to the surface of the particle of the biodegradable polymer standard specimen and cultured under microbial culture conditions using a constant temperature stirrer.
The stirrer may be replaced with a magnetic stirrer or a constant temperature water chamber.
The culturing conditions are incubated at a temperature of 20 to 50 DEG C and a rotation speed of 100 to 200 rpm, which is suitable for microbial growth. Optimal culture conditions using the above-mentioned constant temperature stirrer are most preferably incubated at a rotation speed of 160 rpm at 37 캜.
Through the above cultivation, microorganisms adhere to the surface of the biodegradable polymer standard specimen to initiate decomposition activity, and metabolism activities such as hydrolysis of polymer, generation of organic acid, and final gas production are performed through the metabolism of microorganisms.
In the second step (S20), the anaerobic condition is filled with nitrogen (N 2 ) to remove oxygen. Specifically, when analysis is performed under anaerobic conditions, the flask containing the analytical sample, the microbial culture medium, and the anaerobic sludge is purged with nitrogen (N 2 ) to remove oxygen, and the stopper is closed and sealed in a thermostatic stirrer Start culturing.
Next, in a third step (S30), the gas in the flask is sampled with a gas sample syringe, and the amount of produced gas is measured by analyzing the composition of the gas phase. Specifically, after the gas in the flask is sampled with a gas sample syringe while being cultured under the above conditions, the gas phase composition is analyzed by gas chromatography or the like to analyze carbon dioxide (CO 2 ) or methane (CH 4 ) produced in the gas phase Measure production.
The gas end products of the polymer through the mineralization in the aerobic condition is measured, and oxygen are abundantly supplied to the final degradation products of carbon dioxide (C0 2) or methane (CH 4) of the biodegradable polymer standard specimen is carbon dioxide (C0 2 ) Is measured, and it is preferable to measure it on the basis of methane (CH 4 ) under oxygen-poor anaerobic conditions.
The gas chromatography can be replaced with various analyzers capable of detecting the gas components of the carbon dioxide (CO 2 ) and methane (CH 4 ), such as a gas detector and a detector for measuring the final decomposition product of the biodegradable polymer standard specimen Do.
Next, in a fourth step S40, the specific surface area of the standard specimen and the gas generation rate are used to calculate the gas generation rate per specific surface area. Specifically, the gas generation rate per non-surface area is calculated in consideration of the specific surface area and time according to the standard specimen.
Next, in the fifth step (S50), the degree of biodegradation is evaluated using the gas generation rate per non-surface area. Specifically, the calculated rate of gas generation per surface area is indexed to evaluate the degree of biodegradation.
The standardization of the biodegradability test method according to the present invention standardizes the existing biodegradability assessment method which does not have a standard. Specimens are standardized for clear comparison of various test methods, and standardization of the surface area and volume of the standardized specimens enables comparison between biodegradation test methods.
As described above, it is to be understood that the technical structure of the present invention can be embodied in other specific forms without departing from the spirit and essential characteristics of the present invention.
Therefore, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than the foregoing description, All changes or modifications that come within the scope of the equivalent concept are to be construed as being included within the scope of the present invention.
S10. A first step of putting the standard specimen into a closed flask, and a microbial culture medium and anaerobic sludge are introduced into the closed flask
S20. A second step of incubating the flask in a sealed incubator,
S30. A third step of measuring the amount of gas produced by analyzing the composition of the gas phase after collecting the gas in the flask with a gas sample syringe,
S40. A fourth step of calculating the gas generation rate per non-surface area using the specific surface area of the standard specimen and the gas generation amount,
S50. A fifth step of evaluating the degree of biodegradation using the gas generation rate per non-surface area
Claims (6)
Wherein the biodegradable polymer standard specimen includes planar porosity, and a biodegradability evaluation method using the standard specimen for biodegradability evaluation
The biodegradable polymer standard specimen is a standard specimen for biodegradability evaluation, characterized in that the size of the specimen is standardized, and a biodegradability evaluation method using the standard specimen
The biodegradation degree evaluation method includes:
The biodegradable polymer standard specimen is biodegraded as a microorganism, and the gas generation rate of the generated gas is measured.
Wherein the gas generating rate of the biodegradable polymer standard specimen is calculated using the measured surface area of the biodegradable polymer standard specimen and the gas generation rate, Specimen and method for evaluating biodegradability using the same
Wherein the gas generation rate is a gas generation rate of carbon dioxide (CO 2 ) in an aerobic condition and a gas generation rate of methane (CH 4 ) in an anaerobic condition, and Biodegradability assessment method
The biodegradation degree evaluation method includes:
The biodegradable polymer standard specimens are immersed in soil, and the change in molecular weight is measured by gel permeation chromatography (GPC) analysis. The standard molecular weight of the biodegradable polymer specimen is compared with the standard molecular weight of the biodegradable polymer standard specimen. Assessment Methods
The biodegradation degree evaluation method includes:
The biodegradable polymer standard specimen is biodegraded in a solution having a different pH value, and then the difference in weight change is measured, and the biodegradable polymer standard specimen is indexed and compared for comparison. Rating Method
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150057146A KR20160126272A (en) | 2015-04-23 | 2015-04-23 | Polymer for evaluation method of biodegradation and evaluation method of biodegradation using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150057146A KR20160126272A (en) | 2015-04-23 | 2015-04-23 | Polymer for evaluation method of biodegradation and evaluation method of biodegradation using the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20160126272A true KR20160126272A (en) | 2016-11-02 |
Family
ID=57518441
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020150057146A KR20160126272A (en) | 2015-04-23 | 2015-04-23 | Polymer for evaluation method of biodegradation and evaluation method of biodegradation using the same |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR20160126272A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190031863A (en) * | 2017-09-18 | 2019-03-27 | 주식회사 엘지화학 | Apparatus and method for detacting properties of soil |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120048405A (en) | 2010-11-05 | 2012-05-15 | 채명해 | Shredder |
-
2015
- 2015-04-23 KR KR1020150057146A patent/KR20160126272A/en active Search and Examination
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120048405A (en) | 2010-11-05 | 2012-05-15 | 채명해 | Shredder |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190031863A (en) * | 2017-09-18 | 2019-03-27 | 주식회사 엘지화학 | Apparatus and method for detacting properties of soil |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bergman et al. | Microbial carbon mineralisation in an acid surface peat: effects of environmental factors in laboratory incubations | |
| Kim et al. | Effect of particle size and sodium ion concentration on anaerobic thermophilic food waste digestion | |
| Chan et al. | Effects of leachate recirculation on biogas production from landfill co-disposal of municipal solid waste, sewage sludge and marine sediment | |
| Moody et al. | Using biochemical methane potential assays to aid in co-substrate selection for co-digestion | |
| KR20160099910A (en) | Evaluation method of biodegradation | |
| Geng et al. | Simultaneous monitoring of phosphine and of phosphorus species in Taihu Lake sediments and phosphine emission from lake sediments | |
| Li et al. | Influences of carbon sources on N2O production during denitrification in freshwaters: activity, isotopes and functional microbes | |
| Coveney et al. | Bacterial metabolism of algal extracellular carbon | |
| Zhang et al. | Discrepant responses of bacterial community and enzyme activities to conventional and biodegradable microplastics in paddy soil | |
| De Wilde | Biodegradation testing protocols | |
| KR20160126272A (en) | Polymer for evaluation method of biodegradation and evaluation method of biodegradation using the same | |
| Bengtsson et al. | Dissolved organic carbon dynamics in the peat–streamwater interface | |
| Yilmaz | A straightforward method: Biochemical methane potential assay | |
| Top et al. | Investigation of solid waste characteristics in field-scale landfill test cells | |
| CN104480037A (en) | Method for screening phthalate degradation functional floras based on Biolog technique | |
| Greene | Degradation and biodegradation standards for starch-based and other polymeric materials | |
| Putro et al. | Biomethane emissions: Measurement in wastewater pond at palm oil mill by using TGS2611 methane gas sensor | |
| CN103822963A (en) | Method for identifying whether organic matters are utilized by ANAMMOX (anaerobic ammonium oxidation) bacteria in ANAMMOX reactor sludge | |
| Rodríguez-Ballesteros et al. | Comparison of in Batch Aerobic and Anaerobic Processes for the Degradation of Organic Matter in a Tropical Reservoir | |
| CN113624829A (en) | Method for testing nitrate nitrogen oxygen isotope in water sample by using trivalent titanium reduction method | |
| Grima et al. | A new test method for determining biodegradation of plastic material under controlled aerobic conditions in a soil-simulation solid environment | |
| Wan et al. | Permeability decides the effect of antibiotics on sedimentary nitrogen removal in Jiulong River Estuary | |
| Widadri et al. | Effect of C/N ratio variations on the capability of microbes from Muara Karang river sediment in the production of biogas and identification using VITEK 2 | |
| US5674702A (en) | Determination of the toxicity of water using an anaerobic bacterial culture | |
| CN112661374A (en) | Method for improving methane yield in sludge anaerobic digestion process by using micro-plastic |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination |