KR20160105716A - Disposable container for bioreactor - Google Patents
Disposable container for bioreactor Download PDFInfo
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- KR20160105716A KR20160105716A KR1020160021847A KR20160021847A KR20160105716A KR 20160105716 A KR20160105716 A KR 20160105716A KR 1020160021847 A KR1020160021847 A KR 1020160021847A KR 20160021847 A KR20160021847 A KR 20160021847A KR 20160105716 A KR20160105716 A KR 20160105716A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/26—Constructional details, e.g. recesses, hinges flexible
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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Abstract
A disposable container of a cell culture system is disclosed. A disposable container of a cell culture system according to an embodiment of the present invention includes a cover having a cylindrical shape with at least one flexible structure having a bottom open and a cylindrical shape coupled with an open bottom side of the cover, And a base provided with at least one connection port projecting outwardly on the side thereof so that the structure of the supporting structure for self-standing can be minimized and a separate supporting structure for supporting the stirrer is not required, Time, effort and cost can be saved.
Description
The present invention relates to a disposable container for use in a bioreactor, and more particularly, to a disposable container of a bioreactor capable of minimizing the installation of a support structure for supporting the bioreactor and supporting the stirrer itself.
Currently, various kinds of bioreactors including cell incubators are widely used in medicine, pharmacy, biotechnology and the like, such as the manufacture of reagents or vaccines, the development of new drugs, and the treatment and research using stem cells.
Bioreactor refers to a system that artificially reproduces biochemical reaction processes such as decomposition, synthesis, and chemical transformation of substances in the body of a living organism, and is also called a bioreactor.
Therefore, various conditions such as nutrients, temperature, humidity, pH, oxygen and carbon dioxide concentration suitable for the mixing of substances or cultivation of cells are actually maintained for a predetermined period according to various work contents performed by the bioreactor .
In case of using bioreactor to pollute the material that is being produced, it is common to dispose of all expensive materials that are in use. In particular, when a cell is cultured with a bioreactor, it is very rare that the cell itself has an immune system. Therefore, the inside of the bioreactor must be able to be kept in a shielded state so as not to be contaminated. Before the cell culture starts, the bioreactor is autoclaved or gamma-sterilized.
In the case of culturing animal cells, all components of the bioreactor that can be contacted with the cells must be made of an animal-derived component free (ADCF) clean material.
Since the components of the bioreactor having all of the above conditions are quite expensive, a large number of components other than consumables such as various connectors and connectors are repeatedly used.
However, once the operation such as cell culture is completed, the bioreactor can be reused after a number of procedures such as completely removing the residual material of the bioreactor and re-executing the above-mentioned sterilization step. Particularly, in the case of containers in which cells are directly accommodated and cultured, more stringent conditions are applied.
Such a process for reusing the bioreactor has a disadvantage in that a considerable time, cost, and effort are required.
In order to overcome these disadvantages, a disposable container which can be discarded once the cell culture is completed has been proposed.
However, since the proposed disposable container is in the same form as a 'plastic bag' made of a film, there is a disadvantage that a support structure supporting the entirety is required to maintain a constant shape of the container in order to cultivate the cells.
For example, Korean Patent Registration No. 10-1228026 discloses a support structure including a rod connected to the top of a container to support a bag-type container.
In addition, an agitator for circulating nutrients and oxygen is generally installed in the container. However, since the disposable container can not support the agitator, there is a disadvantage that a separate support structure for supporting the agitator must be provided.
For example, Korean Patent Laid-Open Publication No. 10-2012-0108054 discloses that a stirrer installed in a bag is fixed by a support housing.
In addition, the conventional disposable container is provided with a through hole formed in a container film for each portion where nutrients are supplied, waste is discharged, oxygen is supplied, and temperature and pH sensors are connected to each other. It takes a considerable amount of time to check the state, and there is also a possibility that structural defects are generated.
An embodiment of the present invention seeks to provide a disposable container of a bioreactor capable of minimizing the installation of a support structure for supporting a disposable container of a bioreactor and supporting the stirrer by itself.
In the embodiment of the present invention, a connection part for connecting the disposable container of the bioreactor with the sensor for measuring the supply of nutrients, the discharge of waste products, the supply of oxygen, and the temperature and pH is formed in advance, Disposable containers of bioreactors that can save time and effort.
Also, an embodiment of the present invention is to provide a disposable container of a bioreactor in which the efficiency of a biological process can be improved.
Also, an embodiment of the present invention is to provide a disposable container of a bioreactor that can be folded.
According to an aspect of the present invention, there is provided a container comprising: a container-shaped cover made of at least one flexible structural layer and having a bottom open; a container shape coupled to the open lower side of the cover, And a lower end of the cover is coupled to an upper end of an edge of the base. The disposable container of the cell culture system may be provided in the container. At this time, a rounded portion may be formed on the inner lower edge of the base.
The cover may have a shape of a cylinder, an ellipse, a prism, a cone, or a pyramid, and the open top surface of the base may have a shape corresponding to the shape of the open bottom surface of the cover.
The cover and the base may be engaged with each other by fusion of the inner circumferential surface of the lower end of the cover to the outer circumferential surface of the upper end of the base of the cover or the outer circumferential surface of the lower end of the cover may be fused to the inner circumferential surface of the upper end of the base.
In this case, the upper end of the base may be formed with a number of engagement protrusions corresponding to the number of the structural layers along the outer circumference sequentially toward the outward direction or the inward direction of the base. At this time, an engaging protrusion or an engaging groove is formed in a portion of the base which is in contact with one surface or the other surface of the end portion of the structure layer, and one surface or the other surface of the structure layer is provided with the engaging projection or the engaging groove Corresponding engagement grooves or engaging projections can be formed.
Alternatively, a number of insertion grooves corresponding to the number of the structural layers may be sequentially formed in an upper end portion of the base at an edge of the base toward an outward direction or an inward direction of the base, and an end portion of the structure layer Can be inserted. At this time, a supporting rib supporting the end of the inserted structure layer may be protruded from the insertion groove.
The disposable container of the above-described cell culture system may further include the cover and the connection ring interposed in the base.
Here, the outer circumferential surface of the connecting ring is fused to the outer circumferential surface of the lower end portion of the cover, or the inner circumferential surface of the connecting ring is fused to the outer circumferential surface of the lower end portion of the cover, and a fastening protrusion or a fastening groove And a fastening groove corresponding to the fastening protrusion or a fastening protrusion corresponding to the fastening groove may be formed at the upper end of the edge of the base.
The disposable container of the cell culture system may further include a reinforcing ring having an inner circumferential surface welded to an outer circumferential surface of a lower end portion of the cover and a fastening protrusion formed at a lower side thereof, Wherein an engaging rib is protruded upward from an upper end of the base closer to the central portion of the base than the engaging groove, and the engaging rib is formed with a protruding portion, which protrudes upward from the reinforcing ring when the engaging projection is engaged with the engaging groove It can be further projected upward.
In the disposable container of the above-described cell culture system, one or more tensile grooves may be formed in the portion of the upper edge of the base which is fused to the lower end of the cover.
Here, the tensile groove may be a long groove extending in a direction parallel to the center axis of the base.
In the disposable container of the above-described cell culture system, a heat medium circulation channel is formed in the base, and at least one pair of heat medium circulation ports communicating with the heat medium circulation channel may be formed on an outer circumferential surface of the base.
In the disposable container of the above-described cell culture system, the base surrounds the side and bottom of the base and is coupled to the base. A heat medium circulation channel is formed in the inside of the disposable container, and at least one pair of heat medium circulation ports communicating with the heat medium circulation channel A formed base temperature adjusting means may be further included.
In the disposable container of the above-described cell culture system, the cover surrounds the outer circumferential surface of the cover and is supported by the base, and a heat medium circulation channel is formed therein, and at least one pair of heat medium circulation ports communicating with the heat medium circulation channel is formed on the outer circumference thereof The cover temperature adjusting means may be further included.
In the disposable container of the above-described cell culture system, at least one contact wire is formed on the cover, and when a vertical external force is applied to the cover, a portion of the cover where the contact wire is formed is folded, Can be shortened. At this time, the folded portion of the cover can be received inside the base.
In the disposable container of the cell culture system as described above, a fixing protrusion or a fixing groove may be formed in the middle of the inner bottom surface of the base. Here, the seating rib may protrude from the outer circumference of the fixing protrusion, or may have a seating recess.
Or a disposable container of a cell culture system, wherein a cap coupling hole is formed on at least a part of an upper surface of the cover, and a cap coupled to the cap coupling hole is further included, and an edge portion of the cap coupling hole Can be formed. Here, a shaft hole may be formed through the intermediate portion of the cap.
According to the embodiment of the present invention, since the structure of the support structure for self-standing can be minimized and a separate support structure for supporting the stirrer is not required, the time, effort and cost required to construct the bioreactor Can be saved.
In addition, according to the embodiment of the present invention, since the connection part for connecting the various connection pipes is formed in advance, the time and effort required for preparing the work using the bioreactor can be greatly reduced.
In addition, according to the embodiment of the present invention, the formation of dead zones in the bioreactor is minimized, the circulation of the culture liquid and the like is smooth, and the efficiency of the biological process can be improved by minimizing the residual of the final product in which the biological process is completed.
In addition, the disposable container of the bioreactor can be folded so that the space occupied by the disposable container of the bioreactor during transportation or storage can be minimized.
1 is an exploded perspective view of a disposable container of a bioreactor according to a first embodiment of the present invention;
Fig. 2 is a longitudinal sectional view of the disposable container of the bioreactor shown in Fig. 1
3 is an enlarged view of a portion indicated by A in Fig.
Figs. 4 and 5 are views for explaining a modification of the portion shown in Fig. 3
6 is an exploded perspective view of the disposable container of the bioreactor according to the second embodiment of the present invention.
7 is a perspective view of the disposable container of the bioreactor shown in Fig. 6
8 is a longitudinal sectional view taken along line BB in Fig. 7
9 is an exploded perspective view of the disposable container of the bioreactor according to the third embodiment of the present invention.
FIG. 10 is a perspective view of the disposable container of the bioreactor according to the third embodiment of the present invention shown in FIG. 9
11 is a perspective view of a disposable container of a bioreactor according to a fourth embodiment of the present invention
12 is a perspective view of a disposable container of a bioreactor according to a fifth embodiment of the present invention
13 is a longitudinal sectional view along CC line segment of Fig.
14 is an exploded perspective view of the disposable container of the bioreactor according to the sixth embodiment of the present invention
Fig. 15 is a view for explaining the fusing method of the cover and the base shown in Fig. 14
16 is a longitudinal sectional enlarged view of a portion indicated by D in Fig.
17 is a view for explaining a modification of the portion shown in Fig. 16
Fig. 18 is a perspective view of the disposable container of the bioreactor shown in Fig. 14
19 is a view illustrating a state in which the disposable container of the bioreactor shown in Fig. 18 is folded
20 is an exploded perspective view of the disposable container of the bioreactor according to the seventh embodiment of the present invention
Figs. 21 to 24 are views for explaining the fusing method of the cover and the base shown in Fig. 20
25 is an exploded perspective view of the disposable container of the bioreactor according to the eighth embodiment of the present invention
Fig. 26 is a view for explaining a fusing method of the cover and the connecting ring shown in Fig. 25
Fig. 27 is an enlarged longitudinal sectional view of a portion indicated by E in Fig.
28 is an exploded perspective view of the disposable container of the bioreactor according to the ninth embodiment of the present invention
29 is a longitudinal sectional enlarged view of a portion indicated by F in Fig.
30 is a flowchart for explaining a method of manufacturing a disposable container of a bioreactor according to an embodiment of the present invention.
The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated and described in the drawings. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.
In the present specification, as described above, a system for artificially reproducing biochemical reaction processes such as decomposition, synthesis, and chemical transformation of substances in the body of a living organism, as well as a mixer for producing a cell culture medium or a culture solution used for cell culture, Will be collectively referred to as a bioreactor.
For convenience of explanation, the disposable container of the bioreactor will be abbreviated as a disposable container in the detailed description of the present specification, and the description of the disposable container to be described below may be applied to various types of bioreactor The culturing operation will be described as an example.
Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.
Fig. 1 is an exploded perspective view of a
Referring to FIGS. 1 and 2, the
The
The
The
Therefore, although not shown in detail, fastening grooves, fastening protrusions, threads, etc. may be formed on the outer circumferential surface or the outer end of the
The base 130 should be made of a material that does not affect the operation such as cell culture or contamination of the material because it is in direct contact with the material such as the culture solution and the cultured cells contained in the
A fixing
The
The
Although not shown in detail, a plurality of permanent magnets are built in the stirring
A number of electromagnets corresponding to permanent magnets (not shown) are built in the magnetic force driving means 160 so that the stirring
The
For this, the
For reference, although not shown, when the cells to be cultured are sensitive to magnetic force, the stirring driving means 360 may be disposed outside the
On the other hand, the
The base 130 may have a sufficient stiffness in consideration of the load applied to the base 130 according to the capacity of the
Meanwhile, when the
At this time, a plurality of connecting wires having appropriate radiuses of curvature may be previously formed on the
The
The
Therefore, as for the method of bonding the
Therefore, in this specification, it is assumed that the coupling of the
The lower end of the structural layer constituting the
Fig. 3 is an enlarged view of a portion indicated by A in Fig.
Referring to FIG. 3, the
Here, the first
The
For this purpose, the
The
Accordingly, the
Although not shown, when the material accommodated in the
In this embodiment, it is assumed that the
As shown in FIG. 3, the upper end of the
After the
At this time, the fused portion W is made to have a sufficient width so as to have sufficient strength so that even if a substance such as a culture liquid is contained in the
Hereinafter, the heating means or the ultrasonic wave generating means for forming the fused portion W will be referred to as fusing means as described above.
The engagement of the
The
Although not shown in the drawings, the fused portions are formed in the
Accordingly, after the
Referring again to FIG. 2, a
The rounding
In the conventional disposable container mentioned above, the outer wall of the disposable container is wrinkled or wrinkled at the edge portion of the bottom surface, and such a portion frequently acts as a dead zone.
When the above-described dead zone is formed in the
In addition, when the final product is discharged to the outside of the
Particularly, when corners are formed between the inner bottom surface and the side surface of the disposable container, there is a problem that the cells being cultured are destroyed by colliding with the culture medium while flowing along with the culture liquid, thereby lowering the yield.
Therefore, by forming the rounding
The rounding
In general, the rounding
For reference, although not shown, the rounding
In addition, although not shown, the rounding
Fig. 4 and Fig. 5 are views for explaining a modification of the portion shown in Fig. Here, it is also assumed that the
4 and 5, a number of
The
In this modified example, the fused portions Wa, Wb and Wc are sequentially formed on the contact surfaces of the
This modification is for easily forming the fused portions Wa, Wb, Wc by temporarily fixing the
1 and 2, the
Although not shown, the
Accordingly, the outer shape of the open upper surface of the base 130 may have a shape such as a circle, an ellipse, or a polygon corresponding thereto. That is, the upper surface of the base 130 also has the shape of a container having various shapes.
FIG. 6 is an exploded perspective view of the disposable container according to the second embodiment of the present invention, FIG. 7 is a perspective view of the disposable container shown in FIG. 6, and FIG. Respectively.
6 and 7, the
Since the
A
The inner surface of the hollow portion of the cover temperature adjusting means 240 has a shape corresponding to the outer circumferential surface of the
8, a heat
Although not shown, heat medium such as hot water or cold water flows into or out of the heat
The base temperature adjusting means 280 is formed to be coupled to the base 230 to surround the side surface and the bottom surface of the base 230 as shown in FIGS.
At this time, a driving means inserting
6 and 7, a
8, the heat medium
The heat medium is introduced into or discharged from the heat
For reference, the number of the heating
FIG. 9 is an exploded perspective view of a
Referring to FIGS. 9 and 10, the
The
The
The
The
The
The
The joining
In the
For reference, the
FIG. 11 is a perspective view of a
Referring to FIG. 11, a
The
The
The
The
The
The
12 is a perspective view of a disposable container according to a fifth embodiment of the present invention, and FIG. 13 is a longitudinal sectional view taken along line C-C of FIG.
Referring to FIGS. 12 and 13, the
The
A plurality of
That is, the
A fixing
The driving means
Meanwhile, in the
Although not shown, the
14, an exploded
Referring to FIG. 14, the
The
When the
For example, the connecting
A
Since the structure and operation of the
The fixing
The
However, a
On the other hand, after the stirring
Although not shown, the outer circumferential surface of the fixing
The rotation of the agitating
The
The
Fig. 15 is a view for explaining the welding method of the
15 and 16, the lower end portion of the
The fusing
Although not shown in detail, the fusing
A fusing
Therefore, the fusing
That is, the
As a result, the pressure of the
At this time, in order to prevent the
In order for the fused portion between the
Fig. 17 is a view for explaining a modification of the portion shown in Fig.
Referring to FIG. 17, the
The
For reference, the above-described
The
On the other hand, when the engaging
Since the fused portion is a portion formed by pressing and cooling the
16 and 17, when the outer peripheral surface of the
Therefore, the fusing
On the other hand, when the inner peripheral surface of the lower end portion of the
Therefore, the fusion of the
Fig. 18 is a perspective view of the
18, the
The coupling of the
In order to perform various biological processes using the
If the
This is a modification to the external force applied in the direction of the arrow shown in Fig. 19, that is, in the vertical direction of the
At this time, when the folded state of the
Accordingly, when a plurality of
20 is an exploded perspective view of the
Referring to FIG. 20, the
The
A
Since the
The fixing
The latching
The
The
The
The function of the
Figs. 21 to 24 are views for explaining the fusing method of the
Referring to Fig. 21, a top view of
Referring to FIG. 22, a
The fusing
At this time, among the plurality of fusing heads 21 and 25, the
Although not shown, a heater or an ultrasonic vibrator or the like is incorporated in the plurality of fusing heads 21 and 25 in the same manner as the fusing
When the inner circumference of the lower end portion of the
This is because the clearance is formed between the lower end of the
On the other hand, unlike the illustrated case, when the outer peripheral surface of the lower end portion of the
This is also for the purpose of forming a clearance between the lower end of the
However, when the outer peripheral surface of the lower end of the
23, the inner peripheral surface of the lower end of the
At this time, the fusing
In this process, the
At this time, the degree of clearance can be adjusted according to the depth and the number of the
However, when the tensile stress is excessively generated at the lower end of the
24, after the welding of the
Therefore, the fusing heads 21 and 25 are formed so as to fuse the entire overlapped portion of the
When the depth and the number of the
The
25 is an exploded perspective view of a
Referring to FIG. 25, the
The
A
Since the
The
Fig. 26 is a view for explaining a fusing method of the
26, the fusing
The outer peripheral surface of the lower end portion of the
At this time, a locking
26, the
For reference, unlike the illustrated case, if necessary, the inner circumferential surface of the lower end of the
A
At this time, the
Although not shown, a coupling groove may be formed on the lower side of the
In the case where the connecting
Accordingly, the cost and time required for the fusing
For reference, the shape of the lower end of the
28 is an exploded perspective view of a disposable container of a bioreactor according to a ninth embodiment of the present invention, and FIG. 29 is an enlarged view of a longitudinal section of a portion indicated by F in FIG. 28
Referring to Figs. 28 and 29, a
The
A
Since the
The reinforcing
A
The lower end of the
At this time, the engaging
Fusing between the
Since the lower end of the
When the fused portion W2 is formed by fusing between the
The
30 is a flowchart illustrating a method of manufacturing
30, a method of manufacturing a
The rigid body fabrication step S10 may be performed by a method such as injection or the like by using components having high stiffness such as the
The cover making step S20 refers to a step of manufacturing the
The combining step S30 is performed by combining the components manufactured in the above-described rigid body part manufacturing step S10 and the cover manufacturing step S20 using the fusing means 10, 20 and 30 described above, 800 < / RTI >
The sterilizing step S40 refers to the step of sterilizing the
Although not shown, the
For reference, the embodiments described above may be installed so as to be inclined or inverted upside down as necessary. That is, the
This facilitates the installation of the support structures that support the unshown covers 110 to 910 and the
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the scope of the present invention is not limited to the disclosed exemplary embodiments, Other embodiments may easily be proposed by adding, changing, deleting, adding, or the like of a component, but this is also within the scope of the present invention.
10: Fusing means 11, 11a: Fusing head
12: corrugated part 13: fusion welding arm
20: Fusing means 21, 25: Fusing head
22:
30: Fusing means 31: Fusing head
33: fusion welding arm
100: Disposable container of bioreactor
110: cover 111: first structural layer
112: coupling groove 113: second structural layer
114: coupling groove 115: third structure layer
116: coupling groove 130: base
131: connection port 132: fixing projection
134: Rounding
135a, 136a, 137a:
150: stirrer 151: stirring body
153: stirring blade 155:
160: stirring driving means
200: Disposable container of bioreactor
210: cover 230: base
231: connection port 238: support rib
240: Temperature adjusting means 241: Heat medium circulation flow path
242, 243: heat medium circulation port 250: stirrer
260: stirring driving means 280: temperature adjusting means
281: Heat medium
284: port groove 285: driving means insertion port
300: Disposable container of bioreactor
310: Cover 317: Cap coupling ball
330: Base 331: Port
350: stirrer 351: stirring main body
352: shaft coupling hole 353: stirring blade
360: stirring driving means 361: stirring shaft
370: Cap 371:
372: Inlet port 373: Fastening port
374:
400: Disposable container of bioreactor
410: cover 430: base
431: connection port 450: stirrer
460: stirring driving means 461: stirring shaft
470: Cap 471:
472: Inlet port 473: Fastening port
500: disposable container of bioreactor
510: Cover 530: Base
531: Connection port 532: Fixing projection
535: drive means insertion port 536: heat medium circulation flow path
537, 538: Heat medium circulation port
600: disposable container of bioreactor
610: cover 617:
630, 630a: base 631: connection port
632: Fixing projection 633:
638, 638a: latching
650: stirrer 651: stirring body
652: shaft coupling hole 653: stirring blade
670: cap 672: inlet
673: Fastening port
700: Disposable container of bioreactor
710: Cover 730: Base
731: Connection port 732: Fixing projection
738: Retaining jaw 739: Tension groove
800: Disposable container of bioreactor
810: cover 820: connecting ring
838: fastening protrusion 829: fastening jaw
830: Base 831: Connection port
832: Fixing projection 838:
900: Disposable container of bioreactor
910: cover 920: reinforcing ring
928: fastening protrusion 930: base
931: Connection port 932: Fixing projection
938: fastening groove 939: engaging rib
Claims (21)
And a base coupled to the open lower side of the cover and having a container shape with an opened upper surface and one or more connection ports protruding outward on the side surface,
The lower end of the cover is coupled to the upper end of the base
Disposable container of cell culture system.
A rounded portion is formed on an inner lower edge of the base
Disposable container of cell culture system.
Wherein the cover has a shape of a cylinder, an ellipse column, a prism, a cone or a pyramid, and the open top surface of the base has a shape corresponding to the shape of the open bottom surface of the cover
Disposable container of cell culture system.
The coupling of the cover and the base,
The inner peripheral surface of the lower end of the cover is fused to the outer peripheral surface of the upper end of the edge of the base or the outer peripheral surface of the lower end of the cover is fused to the inner peripheral surface of the upper end of the edge of the base
Disposable container of cell culture system.
A number of latching jaws corresponding to the number of the structural layers along the outer periphery are sequentially formed on the upper end of the edge of the base toward the outward direction or the inward direction of the base
Disposable container of cell culture system.
A coupling protrusion or an engaging groove is formed in a portion of the base which is in contact with one surface or the other surface of the end of the structure layer,
A coupling groove or a coupling protrusion corresponding to the coupling protrusion or the coupling groove is formed on one surface or the other surface of the end of the structure layer
Disposable container of cell culture system.
A number of insertion grooves corresponding to the number of the structural layers is sequentially formed in the upper end portion of the base along the outer circumference toward the outward direction or the inward direction of the base,
The end of the structural layer is inserted into the insertion groove
Disposable container of cell culture system.
And a connecting ring interposed between the cover and the base
Disposable container of cell culture system.
The outer circumferential surface of the connecting ring is fused to the outer circumferential surface of the lower end portion of the cover, or the inner circumferential surface of the connecting ring is fused to the outer circumferential surface of the lower end portion of the cover,
A fastening protrusion is formed on the lower side of the connection ring, and a fastening groove corresponding to the fastening protrusion is formed on the upper end of the base
Disposable container of cell culture system.
And a reinforcing ring having an inner circumferential surface welded to an outer circumferential surface of a lower end portion of the cover and a fastening protrusion formed on a lower side thereof,
A coupling groove corresponding to the coupling protrusion is formed at the upper end of the edge of the base,
And an engaging rib is protruded upward from an upper end of the base at a position closer to the center of the base than the engaging groove and the engaging rib is engaged with the engaging groove in the upper direction of the reinforcing ring when the engaging projection is engaged with the engaging groove Protruding
Disposable container of cell culture system.
And at least one tensile groove is formed in a portion of the upper edge of the base which is welded to the lower end of the cover
Disposable container of cell culture system.
Wherein the tensile grooves are elongated grooves extending in a direction parallel to the central axis of the base
Disposable container of cell culture system.
A heat medium circulation channel is formed in the base,
Wherein at least one pair of heat medium circulation ports communicating with the heat medium circulation flow path is formed on an outer circumferential surface of the base
Disposable container of cell culture system.
And a base temperature adjusting means coupled to the base to surround side and bottom surfaces of the base and having a heat medium circulating flow path formed therein and having at least one pair of heat medium circulating ports communicating with the heat medium circulating flow path formed on an outer circumferential surface thereof,
Disposable container of cell culture system.
And a cover temperature adjusting means which surrounds the outer circumferential surface of the cover and is supported by the base, a heat medium circulating flow path is formed in the inside thereof, and at least one pair of heat medium circulation ports communicating with the heat medium circulating flow path is formed on the outer circumferential surface thereof.
Disposable container of cell culture system.
The cover is formed with at least one ground wire,
When a vertical external force is applied to the cover, the portion of the cover where the contact wire is formed is folded and the length of the cover in the vertical direction is shortened
Disposable container of cell culture system.
The folded portion of the cover is received within the base
Disposable container of cell culture system.
A fixing protrusion or a fixing groove is formed in an intermediate portion of an inner bottom surface of the base
Disposable container of cell culture system.
The fixing rib may be formed on the outer periphery of the fixing protrusion such that the mounting rib is protruded or the mounting groove is formed
Disposable container of cell culture system.
And a cap coupled to the cap coupling hole, wherein the cap coupling hole is formed on at least a portion of the upper surface of the cover,
An edge portion of the cap is formed with an engaging portion that fuses with an edge portion of the cap engaging hole
Disposable container of cell culture system.
A shaft hole is formed at an intermediate portion of the cap
Disposable container of cell culture system.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/KR2016/001810 WO2016137231A1 (en) | 2015-02-28 | 2016-02-24 | Disposable container for bioreactor |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020150028733 | 2015-02-28 | ||
KR20150028733 | 2015-02-28 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020180031484A Division KR20180030820A (en) | 2018-03-19 | 2018-03-19 | Disposable container for bioreactor |
Publications (1)
Publication Number | Publication Date |
---|---|
KR20160105716A true KR20160105716A (en) | 2016-09-07 |
Family
ID=56949871
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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KR1020160021847A KR20160105716A (en) | 2015-02-28 | 2016-02-24 | Disposable container for bioreactor |
Country Status (1)
Country | Link |
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KR (1) | KR20160105716A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114011305A (en) * | 2021-11-29 | 2022-02-08 | 上海偲瑞生物医药有限公司 | Disposable stirring system and method |
KR20230050852A (en) * | 2021-10-08 | 2023-04-17 | (주)씨엔에스 | Cell culture device comprising a removable culture vessel and a jacket for circulating water |
KR102638964B1 (en) | 2023-08-24 | 2024-02-21 | 셀베이스 주식회사 | All-in-one container module for single-use bioreactor |
Citations (2)
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KR20120108054A (en) | 2010-03-09 | 2012-10-04 | 이엠디 밀리포어 코포레이션 | Process bag container with sensors |
KR101228026B1 (en) | 2004-02-24 | 2013-01-30 | 프로탈릭스 리미티드 | Cell/tissue culturing device, system and method |
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2016
- 2016-02-24 KR KR1020160021847A patent/KR20160105716A/en active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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KR101228026B1 (en) | 2004-02-24 | 2013-01-30 | 프로탈릭스 리미티드 | Cell/tissue culturing device, system and method |
KR20120108054A (en) | 2010-03-09 | 2012-10-04 | 이엠디 밀리포어 코포레이션 | Process bag container with sensors |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20230050852A (en) * | 2021-10-08 | 2023-04-17 | (주)씨엔에스 | Cell culture device comprising a removable culture vessel and a jacket for circulating water |
CN114011305A (en) * | 2021-11-29 | 2022-02-08 | 上海偲瑞生物医药有限公司 | Disposable stirring system and method |
KR102638964B1 (en) | 2023-08-24 | 2024-02-21 | 셀베이스 주식회사 | All-in-one container module for single-use bioreactor |
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