KR20160101229A - Device of pilot-scale continuous-flow intense pulsed light(IPL) system for sterilizing groundwater using in food industry, and use thereof - Google Patents

Abstract

TECHNICAL FIELD The present invention relates to a continuous optical pulse sterilizing apparatus for sterilizing working water used in the food industry and a method of manufacturing a kimchi using the apparatus. More specifically, when the optical pulse system of the present invention is applied to processing water, It is confirmed that it shows significant sterilization effect and economical efficiency as compared with the case of applying to the individual food. Therefore, when the present invention is applied to the processing water of the individual food which is difficult to apply by the present heat sterilization technology, economical efficiency and a safer food for consumers are provided It can be used to achieve a healthy food culture of the people.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a continuous optical pulse sterilization apparatus for sterilization of food water and a sterilization method thereof,

TECHNICAL FIELD The present invention relates to a continuous optical pulse sterilizing apparatus for sterilizing working water used in the food industry and a method of manufacturing a kimchi using the same.

As a result of increasing human greenhouse gas emissions and impacts on the Earth's climate system, the average global temperature has risen by about 0.6 ° C over the past century and by about 2100 it is expected to rise by 1.4-5.8 ° C And the concentration of carbon dioxide in 2000 was 370 ppm, which was about 30% higher than that of 280 ppm before the Industrial Revolution. In Korea, the average temperature rise over the past 100 years is 1.5 ℃, which is higher than the global average temperature rise.

Research on biological systems, including humans, due to climate change is actively underway, but relatively little attention is given to the food industry. In terms of food, it is mainly illuminated from the viewpoint of food security, (Food Safety).

Since the concept of environmental problems has expanded to the concept of Environmental Sound Sustainable Development (ESSD) since the United Nations Conference on Environment and Development (UNCED) in 1992, The solution to environmental problems including the occurrence is also changing.

In view of the solution, in the past, end-of-life technologies to solve environmental problems have been mainly focused on reducing final wastes or emissions, but currently, pollutant emission sources such as the use of raw materials that reduce pollutant emissions To prevent or reduce pollution. The Intergovernmental Panel on Climate Change (IPCC) has proposed a harmonization of greenhouse gas mitigation and adaptation to climate change.

In accordance with the Kyoto Protocol of the United Nations Framework Convention on Climate Change (UNFCCC), anthropogenic greenhouse gas emissions must be reduced by at least 5% (as of 1990) by 2012, Reduction efforts are required. The consumption rate of processed foods tends to increase as the number of processed foods increases. Currently, Korea consumes about 40% of its processed food in spending on food, excluding eating out, and the developed countries such as the US and Canada account for about 90%.

In order to improve the preservability of food, the food industry has traditionally used chemical methods such as heating, drying, concentration, freezing, and chemical preservatives, and these traditional food processing methods have been applied to the development of food industry in Korea It has been found that green growth, which is a national issue in recent years, has a negative impact on the generation of carbon dioxide and minimizing environmental pollution.

Therefore, it is expected that there will be a rapid increase in energy costs due to the strong regulation of carbon dioxide emissions worldwide and the rise in international oil prices. Therefore, in order to enhance international competitiveness of food processing products, development of various environmentally friendly energy- And to secure superiority in international food technology competition. Especially, reduction of carbon production and energy saving through improvement of food processing process are considered to be very important for reduction of product cost, securing of international competitiveness and increase of export.

The development of eco-friendly carbon-cutting food processing technology is required to increase the income of farm households due to the high value-added of processed foods of agricultural and marine products and to activate the economy of farming and fishing villages. In particular, securing hygienic safety through environment- It is expected that it will not only increase competitiveness but also develop into high value-added industries.

There are many studies on non-thermal process and minimal processing which are expected to make a great contribution to carbon dioxide generation and minimization of environmental pollution in the food industry. And commercialization is being carried out. Among the non-heat treatment processing technologies that are currently being studied to achieve commercialization in place of conventional heat treatment in the food industry, there are intense light pulses, high voltage pulsed electric fields, oscillating magnetic fields, ultrasound, irradiation, high pressure processing, microwave, radio frequency, ohmic heating, and the like. And natural bacteriostatic agents using bacteriocin and the like.

Particularly, the optical pulse technique is referred to as various names such as "intensed light pulse", "high-intensity pulsed light", "pulsed white light (WHL)", "broad- Is in the range of 170 - 2600 nm and is distinguished from conventional UV disinfection in that it includes not only ultraviolet (UV) region but also near-infrared (NIR) region. A pulse having an energy density in the range of 0.01 to 50 J / cm ² is applied to the material in the form of a flash of 1 to 20 times at intervals of 1 to 0.1 seconds in the optical pulse processing, Typically a clear fused quartz tube filled with Xenon at a pressure of about 450 torr. The fundamental principle of this technology is to sterilize the surface of food or reduce the number of surface microorganisms by applying intense light of all wavelengths in a very short time. It is used to extend the shelf life of the product and to improve the quality. It can be used not only for sterilization, but also for sterilization of packaging materials and transparent chemicals.

The sterilization power of optical pulse technology is determined by the wavelength of light, the intensity, the number and period of pulses, the distance between the sample and light source, the type of packaging material and food, the transparency and color of liquid sample, The number of lamps, the arrangement and the pulse cycle are different. The mechanism of microbial killing by optical pulse technology has not been established yet, but as in the case of UV sterilization, it is believed that microorganisms are destroyed by destroying the DNA structure of the cells. However, in case of UV sterilization, it is very probable that the damaged DNA is recovered to a normal state by the cell repair system being activated in a specific environment. However, since the optical pulse sterilization is greatly damaged than the UV sterilization, Is much less likely to be recovered and is therefore known to be able to effectively control microorganisms as compared to UV sterilization.

Currently, research on optical pulse technology is very limited in Korea. However, in some developed countries such as USA, optical pulse technology is applied to the surface disinfection of packaging materials, foods, and medicines in a small scale.

PurePulse Technologies of the United States developed the optical pulse device PureBright, which is applied to the disinfection of medicines, medical devices, packaging materials, bottled water, etc. The pulse strength generated by the device developed by this company is the sun It is reported that it is about 20,000 times as much as the ray. It is also known that this device is widely used to examine the bactericidal effect against various microorganisms such as various kinds of bacteria (host cells and spores), fungi, and viruses.

This device was applied to the sterilization of various kinds of foods and packaging materials. Generally, tomatoes are easy to retreat even after refrigerated storage. However, when refrigerated after applying PureBright device, tomatoes were stored for about 30 days freshly, Was used for sterilization after optical pulse sterilization, but the bread was kept fresh for more than 2 weeks. However, in the case of the bread which had not been sterilized by optical pulse sterilization, mold was frequently found in the same period.

Dunn et al. Reported that they could extend shelf life by effectively sterilizing molds by applying optical pulse sterilization to various bakery products. When shrimp were stored in refrigerator for 7 days after optical pulse treatment, they were in good condition . However, when stored in a refrigerator without optical pulse treatment, discoloration and deodorization occurred, and it became unhealthy. Escherichia , which is present in various foods such as chicken meat, hot dogs, and cheese, coli , Staphylococcus aureus , Bacillus subtilis , Saccharomyces cerevisiae , Listeria It has been reported that the application of optical pulse technology to the sterilization of innocua , Salmonella , Pseudomonas, etc. can effectively extend the shelf life while minimizing sensory changes.

The US Food and Drug Administration (FDA) has established a recommendation for the use of optical pulse sterilization technology (Food and Drug Administration Issues Approval for Pulsed UV Light in the Production, Processing and Handling of Food [Code 21CFR179.41]).

Although these cutting-edge eco-friendly food processing technologies have advantages in reducing carbon dioxide generation, energy savings, and quality of raw materials and products, they are different from each other depending on the target food, and they can not be applied to all food products at the same time have. The customized application of individual food products, which are difficult to apply with the current heat sterilization technology, is not only for solving the problems of the food industry but also for providing a safer food for consumers, It is a matter to be done in a moment.

As a result of efforts to develop a water sterilization system for processing used in food processing, the present inventors have found that continuous-flow intense pulsed light (IPL) systems can control bacteria and viruses, The present invention has been accomplished by confirming that the sterilizing effect is very effective for the ground water used as food water in the food industry and is more economical than the conventional chlorine disinfection system and ozone sterilizing system.

It is an object of the present invention to provide a method and an apparatus for controlling bacteria and viruses, which can easily recognize data, and which is highly effective in disinfecting groundwater used as a food water in the food industry and is more economical than a conventional chlorine disinfection system and an ozone sterilizing apparatus The present invention relates to a continuous optical pulse sterilizing apparatus for sterilizing processing water and a method of manufacturing a kimchi using the same.

In order to achieve the above object, an optical pulse sterilizer according to the present invention comprises:

A chamber formed in a hollow shape and extending in a vertical direction; A lamp channel formed by vertically arranging a plurality of lamps for generating a continuous optical pulse through the chamber; An inlet pipe connected to a lower portion of the chamber and allowing water to flow into the chamber; And a discharge pipe connected to the upper portion of the chamber and discharging the water treated by the plurality of lamps.

According to one aspect of the present invention, the chamber is formed in a cylindrical shape, and a chamber inlet port through which the inlet pipe is connected is formed at a lower portion of the chamber, and a chamber outlet port through which the outlet pipe is connected is formed at an upper portion of the chamber .

According to an aspect of the present invention, the chamber is formed with a window for monitoring water to be treated inside the chamber, and the window is formed on the lamp channel.

According to an aspect of the present invention, a sensor holder on which an energy density measuring sensor is mounted and an air vent through which air in the chamber is discharged are formed on the upper surface of the chamber.

According to an aspect of the present invention, the at least two lamp channels are provided, and the at least two lamp channels are formed at equal intervals on the outer circumferential surface of the chamber.

According to an aspect of the present invention, the optical pulse sterilizing apparatus has four lamp channels, and each lamp channel is formed at equal intervals on the outer circumferential surface of the chamber.

According to one aspect of the present invention, the inlet pipe includes a water inlet through which water flows, and a filter unit for filtering foreign substances contained in the introduced water (the first filter is a pore size filter of 100 micrometers, The second filter has a function of removing small particles as a filter having a pore size of 10 micrometers), and a flow meter for measuring the flow rate of the introduced water.

According to an aspect of the present invention, the optical pulse sterilizing apparatus includes a power supply unit for supplying power to the plurality of lamps, a water pump for flowing the water introduced into the inlet pipe, And a controller.

According to an aspect of the present invention, the controller includes a trigger circuit that can simultaneously or sequentially control the operation of the plurality of lamps.

According to an aspect of the present invention, the controller includes a transmission module capable of transmitting a control signal to the power supply unit, and the power supply unit includes a reception module capable of receiving the control signal, .

According to an aspect of the present invention, the power supply unit includes a field effect transistor (FET), which is a high voltage switch, and controls the duty ratio and the frequency of the continuous optical pulse by the FET.

According to an aspect of the present invention, the optical pulse sterilizing apparatus has a plurality of lamp channels, and the controller can selectively operate each lamp channel.

In addition, the present invention provides a method for manufacturing a kimchi comprising the step of treating the optical pulse sterilizing apparatus to water for processing during the manufacturing process.

According to the optical pulse sterilizer of the embodiment of the present invention, it is possible to control harmful bacteria and viruses, to easily recognize the data, to provide a sterilizing effect on the ground water used as food water in the food industry, Disinfecting system and the ozone sterilizing apparatus of the present invention. Further, as a result of application of the processing water used in the gimjejo using the optical pulse sterilization apparatus of the present invention, Can be usefully used in the gimjejo process.

1 is a front view showing an optical pulse sterilizing apparatus according to an embodiment of the present invention.
2 is a circuit diagram illustrating a trigger circuit used in an optical pulse sterilizer according to an embodiment of the present invention.
3 is a diagram illustrating a connection relationship between a power supply unit of the optical pulse sterilizer and a lamp and a trigger circuit according to an embodiment of the present invention.
4 is a photograph showing a power supply unit of the optical pulse sterilizer according to an embodiment of the present invention.
5 and 6 are photographs showing a display screen of a controller of the optical pulse sterilizer according to an embodiment of the present invention.
7 is a photograph showing an optical pulse sterilizing apparatus according to an embodiment of the present invention.
8 is a photograph showing the inside of the chamber of the optical pulse sterilizing apparatus according to an embodiment of the present invention.
9 is a plan view showing the inside of a chamber of the optical pulse sterilizer according to an embodiment of the present invention.
FIG. 10 and FIG. 11 are graphs showing the effect of virus reduction when sterilized using the optical pulse sterilizer according to an embodiment of the present invention.
12 is a graph showing sterilizing effect of processing water using the optical pulse sterilizer according to an embodiment of the present invention.
13 is a graph showing the results of sensory evaluation of dried laver prepared using processing water using the optical pulse sterilizer of the present invention.

Specific structural and functional descriptions of embodiments according to the concepts of the present invention disclosed in this specification or application are merely illustrative for the purpose of illustrating embodiments in accordance with the concepts of the present invention, The examples may be embodied in various forms and should not be construed as limited to the embodiments set forth herein or in the application.

The embodiments according to the inventive concept are capable of various modifications and may take various forms, so that specific embodiments are illustrated in the drawings and described in detail in this specification or application. It is to be understood, however, that it is not intended to limit the embodiments according to the concepts of the present invention to the particular forms of disclosure, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

The terms first and / or second, etc. may be used to describe various elements, but the elements should not be limited by the terms. The terms are intended to distinguish one element from another, for example, without departing from the scope of the invention in accordance with the concepts of the present invention, the first element may be termed the second element, The second component may also be referred to as a first component.

It is to be understood that when an element is referred to as being "connected" or "connected" to another element, it may be directly connected or connected to the other element, . On the other hand, when an element is referred to as being "directly connected" or "directly connected" to another element, it should be understood that there are no other elements in between. Other expressions that describe the relationship between components, such as "between" and "between" or "neighboring to" and "directly adjacent to" should be interpreted as well.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises ",or" having ", and the like, are intended to specify the presence of stated features, integers, steps, operations, elements, , Steps, operations, components, parts, or combinations thereof, as a matter of principle.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as ideal or overly formal in the sense of the art unless explicitly defined herein Do not.

2 is a circuit diagram illustrating a trigger circuit used in an optical pulse sterilizing apparatus according to an embodiment of the present invention. FIG. 4 is a photograph showing a power supply unit of the optical pulse sterilizer according to an embodiment of the present invention. FIG. 4 is a view showing a power supply unit of the optical pulse sterilizer according to an embodiment of the present invention. 5 and 6 are photographs showing a display screen of the controller of the optical pulse sterilizer according to an embodiment of the present invention, FIG. 7 is a photograph showing the optical pulse sterilizer according to an embodiment of the present invention, FIG. 3 is a photograph showing the interior of the chamber of the optical pulse sterilizer according to one embodiment of the present invention. FIG. 9 is a plan view showing the inside of a chamber of the optical pulse sterilizer according to an embodiment of the present invention.

1, an optical pulse sterilizing apparatus according to an embodiment of the present invention includes a chamber 100, a lamp channel 200, an inflow tube 300, and a discharge tube 400. As shown in FIG. Further, it may further include a power supply unit 500, a water pump 600, and a controller 700.

The chamber 100 is formed in a hollow shape, for example, extending in a vertical direction in a cylindrical shape. That is, the inside of the chamber is empty, and the water introduced through the inflow tube 300 is sterilized by a plurality of lamps 210 to 250 located inside the chamber, The water is discharged to the discharge pipe (400) through the chamber outlet (120). A chamber inlet 110 is formed in the lower portion of the chamber 100 and a chamber outlet 120 is formed in the upper portion of the chamber 100 to connect the outlet pipe 400 to the chamber.

Meanwhile, a window 130 is formed in the chamber 100 to monitor the water to be treated in the chamber. The window 130 is preferably made of a transparent material, for example, glass. The window 130 may be formed at a predetermined position of the chamber 100 and may be formed at a position corresponding to the upper position of the lamp 250 located at the uppermost position, . And is formed at a position corresponding to the chamber outlet 120, so that it is possible to monitor the treatment process of the water even outside.

A sensor holder 140 on which an energy density measuring sensor is mounted is formed on the upper surface of the chamber 100. The energy density measuring sensor performs a function of measuring the amount of light of each wavelength of the irradiated light. An air vent 150 is formed on the upper surface of the chamber 100 to allow the air in the chamber, which is generated as a result of water treatment of the water, to be discharged to the outside.

The lamp channel 200 includes a plurality of lamps 210 to 250 for generating continuous optical pulses. The plurality of lamps 210 to 250 may be arranged in the vertical direction of the chamber 100. They may be arranged in the same line in the vertical direction, or may be arranged in the zigzag shape, as in the drawings. 8 and 9, the lamps 210 to 250 are formed through the chamber 100 and protrude from the inner wall of the chamber 100 by a predetermined length. The water introduced from the lower part of the chamber is sterilized by the lamps 210 to 250 as it enters the chamber.

It is preferable that two or more lamp channels are formed on the outer circumferential surface of the chamber 100, and two or more lamp channels are formed on the outer circumferential surface of the chamber 100 at equal intervals. In the drawing, four lamp channels (front lamp, rear lamp, and one lamp channel on both sides) are illustrated, and each lamp channel has five lamps. Here, lamps 210 to 250 are lamps filled with Xenon gas, for example, a flash lamp (NL 4006, Heraeus Noblelight, Cambridge, UK) of Xenon XAP series.

The lamp channel 200 may be selectively operated by a controller 700 described later. Also, the controller 700 can remotely control each of the lamps 210 to 250 in the lamp channel 200 in a wireless manner. This will be described later.

The inlet pipe 300 includes a water inlet 310 through which water flows and a filter unit through which the foreign substances contained in the introduced water are filtered (the first filter is a pore size filter of 100 micrometers, The second filter has a function of removing small particles as a filter having a pore size of 10 micrometers) and a flow meter 340 for measuring the flow rate of the introduced water.

The first filter 320 functions to filter foreign matter of a large size contained in the introduced water. In addition, the second filter 330 functions to filter foreign matter of small size included in the introduced water. On the other hand, the water introduced through the water inlet 310 rises in the vertical direction by the water pump 600 and flows inside the inlet pipe 300. The flow rate of the introduced water can be controlled by controlling the power of the water pump 600, and the controlled flow rate can be confirmed through the flow meter 340.

The discharge pipe 400 is connected to a chamber discharge port 120 formed in the upper part of the chamber 100 so that the water treated by the lamps 210 to 250 is discharged.

The power supply unit 500 supplies power to the plurality of lamps. The power supply unit 500 includes a field effect transistor (FET), which is a high voltage switch, and controls the duty ratio and the frequency of the continuous optical pulse generated from the lamps 210 to 250 by the FET.

As shown in FIG. 3, the power supply unit 500 converts a single-phase 220 V or three-phase 380 V alternating current into a desired voltage through a transformer and rectifies it to supply DC to each of the lamps 210 to 250. Each of the lamps 210 to 250 generates a continuous light pulse simultaneously or sequentially by a trigger circuit as shown in Fig.

4 is a photograph showing a power supply unit 500 of an optical pulse sterilizer according to an embodiment of the present invention. For example, each of the four lamp channels 200 including the lamps 210 to 250 In order to supply power, four power supply units can be implemented to supply power to a total of 20 lamps. Each power supply can control the duty ratio of the pulse and control the frequency with a high voltage switch FET.

The controller 700 controls the operation of the lamp channel. That is, the power supply unit 500 controls the power supplied to the lamps 210 to 250 to be in the form of pulses to control on / off of the lamp channel, and controls the frequency of the lamps 210 to 250, . By doing so, it is possible to control the sterilizing power against microorganisms and harmful bacteria contained in the water.

5 is a photograph showing an example of the controller 700. Referring to FIG. The controller 700 includes a transmission module capable of transmitting a control signal to the power supply unit. At this time, the power supply unit includes a receiving module capable of receiving the control signal. By doing so, the lamps can be remotely controlled by the controller 700 even if the controller 700 and the lamps are not connected by a cable. The controller 700 shown in FIG. 5 shows an interface for selectively turning on / off the corresponding power supply to control each lamp channel. In FIG. 5, the lamp channels 1 to 3 are off and the lamp channel 4 is on. Accordingly, only the power supply for supplying power to the lamp channel 4 is turned on, so that only the lamp channel 4 generates continuous pulse light for sterilization. By selectively turning on / off the power of the lamp channel, it is possible to control the degree of sterilizing power according to the kinds of microorganisms and harmful bacteria contained in the water, thereby preventing excessive power wastage.

6 is a photograph showing a display screen for controlling the entire optical pulse sterilizer according to an embodiment of the present invention. In order to set the optical pulse power remotely from this screen, it is possible to set detailed conditions such as voltage, frequency, pulse duty ratio, operation time, control direction of motor, control amount and so on.

Hereinafter, an optical pulse sterilizing effect using the optical pulse sterilizer according to an embodiment of the present invention will be described.

In order to confirm the retention time according to the flow rate of the tap water prior to the full-scale inoculation experiment, the stagnation time according to the flow rate was measured. As a result, as shown in Table 1, the stagnation time according to each flow rate was shown (Table 1).

1. Materials and Methods

1.1 Used strain  And culture conditions

In order to measure the bactericidal effect of the continuous optical pulse sterilizer, the strain was cultured as follows.

Specifically, the strain is Escherichia coli C600. The inoculum was inoculated into a 50 ml Erlenmeyer flask containing 15 ml of nutrient broth after 12 colonies from the strain-storing flat culture medium and then pre-cultured at 37 ° C for 24 hours at 200 rpm. 10 ml of the pre-culture solution was inoculated into an Erlenmeyer flask containing 1000 ml of nutrient broth, and cultured at the same temperature for 5 hours to use the latter strain of the logarithmic growth phase for the experiment. At this time, the final cell density was 1.010 ⁵ CFU / ml.

1.2 Sampling and Number of bacteria  Measure

The bacterial counts were measured for each experiment using the strains described above.

Specifically, in each experiment, two samples of 20 to 30 ml were collected in a sterilized conical tube. The number of bacteria was measured within one hour after the sample was collected and the number of bacteria before and after IPL treatment was calculated. Samples were decanted into 0.85% saline and plated on nutrient agar plates. Plates were incubated for 48 hours at 37 ° C, then 30-300 colonies were counted and expressed as CFU / ml.

2. Optical pulse  Identification of virus culture conditions for use of sterilization device

2.1 Host cell culture conditions

RAW 264.7 cells were used as the host cells and 10% (v / v) fetal bovine serum, 1% (v / v) nonessential amino acids, 1% (v / v) penicillin The cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing penicillin streptomycin and 1% (v / v) 100 mM sodium pyruvate. RAW 264.7 cells were subcultured in a 75 cm ² cell culture flask. Once monolayers were formed, cells were harvested from the flask using a scraper and centrifuged at 1,500 rpm for 5 min. After separation, the supernatant was removed, cells were loosened with DMEM, and the cells were cultured in a 75 cm ² cell culture flask at 37 ° C in a 5% CO 2 incubator.

2.2 Virus Culture

RAW 264.7 cells in which monolayer was cultured as described above were inoculated with Murine norovirus (MNV), a surrogate of Norovirus, and cultured at 37 ° C in 5% CO ² After incubation for 1 hour in an incubator, DMEM was added and incubated at 37 ° C in a 5% CO ² incubator for 48 hours. When the cell-lesion effect has occurred and the adherent cells have disappeared, the culture is placed in centrifugal Filters (Amicon Ultra-15), centrifuged at 3,000 rpm for 30 minutes and the supernatant containing the virus is centrifuged in a cryogenic vial ) And stored in a -70 refrigerator.

2.3 Plaque analysis ( Plaque assay )

The virus suspension was decanted with phosphate buffered saline (PBS) and inoculated with 1 ml of RAW 264.7 cells (cultured in 6-well tissue culture) in monolayer, in% CO ² incubator and incubated for 1 hour. After culturing, 2 × DMEM and 2% (w / v) agarose gel were mixed at a ratio of 1: 1, and the cells were subdivided into 3 ml and cultured for 48 hours until a plaque was formed. When plaques were formed, they were stained with a neutral red for one hour and then dried to determine the number of plaques and calculate plaque forming units (PFU) per ml.

2.4 Optical pulse  process( Lab - scale  or Pilot - scale )

In order to measure the bactericidal effect of the Lab-scale optical pulse device against the virus, the following experiment was conducted.

Specifically, a virus dilution was prepared by diluting virus stocks with a titer of 10 ⁶ to 10 ⁷ PFU / in PBS. 5 ml of the virus dilution was placed in a petri dish (50 x 15 mm), and subjected to light pulse treatment under various conditions of a voltage of 800 to 1800 V and a treatment time of 10 to 50 seconds.

In order to measure the bactericidal effect of the pilot-scale continuous optical pulse device against the virus, 10 ml of virus stock having a titer of 10 ⁶ to 10 ⁷ PFU / was diluted in 100 L of water, And treated with an underground water sterilizer at different treatment times (1 min 29 sec, 2 min 59 sec).

< Experimental Example  1> Piolt - scale  Continuous Optical pulse  Verification of sterilization effect using sterilization device

<1-1> Continuous process in Kim's manufacturing plant Optical pulse  Identification of sterilization effect using sterilization device

In order to verify the sterilization effect using the Piolt-scale continuous optical pulse sterilizer, the following experiment was performed.

Specifically, we visited dry kimchi manufacturers to identify the microbial status of dried kimchi.

First, about 10 ⁴ CFU / g of Escherichia coli was adhered to the surface of the sample taken from the sea, and there was no separate sterilization process during the manufacturing process, and it was confirmed that the product remained in the finished product (dried laver).

Thus, the inventors of the present invention treated the pilot-scale groundwater continuous light pulsing system of the present invention at 5 Hz and 1200 V in the processing water used in the drying process, And then washed with the processing water to which the optical pulse of the present invention is applied. The above-mentioned three-folded stems are washed 2 to 3 times to remove foreign matter, Dried (Kim) (experimental group) was prepared through a forming step in which it was spread in the form of dried laver.

In addition, apart from the above method, the seaweed collected in the sea in front of Seocheon is finely divided to a size that does not sink when it is submerged in water. After washing with ground water and removing foreign matter, The pulsed system was treated at a treatment condition of 5 Hz and 1200 V, and then supplied to a molding apparatus to form a dried kimchi (comparative group) through a molding step in which it spreads out in the form of dried kimchi.

As a result, E. coli was not detected in the dry laver (experimental group) prepared using the processing water using the Pilot-scale continuous groundwater disinfection light pulse system of the present invention, while the test group was found to contain 10 ² CFU / g of E. coli , It was confirmed that the application of the optical pulse system of the present invention to the processing water showed significant effects as compared with the case of directly applying to the individual food. Accordingly, it has been confirmed that when the present invention is applied to the processing water of individual food which is difficult to apply by the current heat sterilization technology, it can be used for achieving healthy food culture of the nation by providing more safe food to consumers .

On the other hand, in the light pulse system of pilot-scale continuous groundwater disinfection, steaming was carried out using the control Kim method according to the number of lamps at the treatment conditions of 5 Hz and 1200 V, The energy densities are shown in Table 2 (Table 2).

As a result, as shown in Fig. 10, the higher the energy density of the sterilizing apparatus and the longer the treatment time, the higher the mortality rate. That is, after the treatment at 5 Hz, 4 minutes and 50 seconds at an energy density of 8.64 J / cm ^2, the result of about 2 log killings was obtained. The treatment was performed at 11.27 J / cm ² energy density at 5 Hz for 4 minutes and 50 seconds The results of the killing of about 3 log were obtained and the killing of about 4.8 log after treatment at 5 Hz, 4 minutes and 50 seconds at the energy density of 14.71 J / cm ² was obtained. In Fig. 10, &amp; cir &amp; denotes a case where five lamps are used, &amp;thetas;, and &amp;

<1-2> Sensory evaluation

The sensory evaluations were performed on the experimental group prepared in <1-1> and the dried kimchi (common kimchi) prepared in the same manner as in the present invention, but without a separate sterilization process in the manufacturing process.

Specifically, the sensory evaluation was performed on 50 women in the 20 to 60 age group in front of Seoul Station, and the taste, aroma, appearance, and overall acceptability were confirmed by the 5-point rating method, and the average score of 50 persons was shown in FIG. 13 : Bad, 2: a little bad, 3: normal, 4: a little good, 5: good.

As a result, as shown in FIG. 13, the taste and flavor of the experimental group and the common kimchi obtained similar scores, but since the dry kimchi of the present invention obtained a significant score on the outer part, It was confirmed that the dried laver of the invention contributes to the health of consumers and is in conformity with the preferences of consumers (Fig. 13).

<1-3> In the food industry of three companies, Optical pulse  Identification of sterilization effect using sterilization device

In order to verify the water sterilization effect for the processing of food industry by using the Piolt-scale continuous optical pulse sterilizer, the following experiment was conducted.

Specifically, 400 liters of groundwater were sampled from three food industries using groundwater as processing water and sterilized with 15 lamps (energy density: 14.71 J / cm ² ) using a pilot-scale continuous pulse sterilizer Respectively.

As a result, as shown in Table 3, the groundwater collected from the food industry A and B was low in contamination level at an initial number of bacteria of less than 10 ³ CFU / ml and died at a retention time of less than 2 minutes.

As shown in Table 3, the initial contamination level of groundwater in the food industry C was somewhat high, and the sterilization effect was reduced by 3 log when the stagnation time was 4 minutes and 30 seconds. In addition, FIG. 12 shows the effect of groundwater sterilization on the food industry C with respect to the stagnation time, and it was confirmed that the sterilization degree tends to increase as the treatment time increases (see FIG. 12). Therefore, it is confirmed that the application of the continuous optical pulse sterilization device developed by the present research team to the groundwater sterilization which is used as food water in the food industry is very effective, and the condition that the bacteria are completely killed when the initial pollution degree of groundwater is high is set It is necessary to increase the processing time or to increase the light intensity.

< Experimental Example  2> Optical pulse  Sterilization-induced norovirus ( norovirus ; MNV ) Reduction  Check the effect

<2-1> Confirmation of virus reduction effect using Lab-scale optical pulse sterilizer

In order to examine the effect of reducing the Norovirus, RAW 264.7 cells were inoculated with Norovirus surrogate murine norovirus (MNV) in the same manner as in the virus culture (2.2 virus culture) The effect of MNV reduction before and after photic pulse sterilization was confirmed by observation through a plaque assay.

As a result, as shown in FIG. 11, when the treatment was carried out at 1800 V for 30 seconds using a lab-scale optical pulse sterilizer, it was reduced by 6.5 log (see FIG. 11).

<2-2> Verification of virus reduction effect using pilot-scale optical pulse sterilizer

In order to investigate the effect of reducing the norovirus, 100 l of water was inoculated with murine norovirus (MNV) in the same manner as in the above virus culture (2.2 virus culture), followed by the use of a pilot-scale continuous light pulse sterilizer And the virus reduction effect was confirmed.

As a result, it was confirmed that when the retention time was set to about 3 minutes with 15 lamps (energy density: 14.71 J / cm ² ) turned on as shown in Table 4, it was confirmed that the lamps were killed (Table 4).

<Experimental Example 3> Comparison of existing sterilization apparatus and optical pulse sterilization apparatus for groundwater

<3-1> Confirmatory comparison between chlorine disinfection system and optical pulse sterilizer

In order to analyze the economical efficiency of the optical pulse groundwater disinfection system for the food industry, it was compared with chlorine disinfection system.

Specifically, as shown in Table 5, the facility cost was calculated by taking into consideration the installation cost of the chlorine disinfection system including the electric work and the piping construction, and the IPL sterilization system, and the operation cost was taken into consideration in the case of the chlorine disinfection system, In case of IPL disinfection device, lamp replacement cost, power ratio and maintenance cost are considered. The electricity cost was calculated by applying the required electricity for each system and the average electricity price of KEPCO (54 won / kw). The facility cost of the chlorination system was estimated to be 65,000,000 won with reference to the data of the existing facilities and the facility cost of the IPL disinfection unit was assumed to be fixed by optical pulse treatment conditions (voltage, pulse width, frequency, etc.) Respectively. The above-mentioned cost can be produced at a cost as low as 2/3 if the mass production system is more than 10 units. The chlorine disinfection system and the IPL disinfection system are compared with each other. The economic life time of the investment facilities of the two systems is assumed to be 10 years and the sensory depreciation is assumed to occur at a constant rate every year.

The total sales management cost for production and the total sales management cost for production are 70% of the sales of the year with reference to the data of the dry kimchi manufacturer, The labor cost is assumed to be 90 million won per year, and the tax on sales profit is set at 33%. Considering that the new technology is applied to the optical pulse sterilization device, the research and development cost of the first year of technology application was further increased to 5,000,000 won (Table 5).

As a result, as shown in Table 6, when analyzed the discounted cash flow return on investment (ROI) when the two systems were applied to the food industry field using the data in Table 5, the chlorine disinfection system 27.0% in the case of the light pulse sterilization apparatus, and 34.7% in the case of the optical pulse sterilization apparatus. Therefore, the introduction of the optical pulse groundwater sterilization device to practical industry could have sufficient economical efficiency. The introduction of optical pulse groundwater sterilization equipment in the field of food industry has the advantage of improving the quality and safety of processed food in terms of replacing the existing chemical sterilization method with physical disinfection method as well as the numerical difference (7.7% (Table 6).

 <3-2> Ozone disinfection device Optical pulse  Comparison of sterilization devices

The following experiment was conducted to compare the economical effects of fresh water, which is a washing water using a conventional ozone sterilizing apparatus, and the optical pulse sterilizing apparatus of the present invention at a food factory.

Specifically, although the ozone sterilization method itself is not a problem, bromate, which is a potential carcinogen caused by the ozone sterilization method, has become a problem. The bromine ion in the groundwater is converted into the form of bromate, which is a potential carcinogen by the ozone sterilization, or the odor remains after sterilization. Instead of ozone sterilization, the optical pulse technology, a non-heat sterilization technology, was applied to a dry-lawn manufacturing plant that uses ground water as the wash water. We analyzed the production costs that would be incurred when applying the continuous optical pulse sterilization device to the industrial field and compared the existing sterilization technology and its economical efficiency considering the sales volume which is expected to increase. (ROI) prior to deciding whether to invest in new technology for optical pulse technology. Assuming the introduction of a continuous optical pulse groundwater sterilization device and estimating the necessary cost, the purchase cost and other investment cost for the optical pulse processing device is 100,000,000 won and the economic life time for this investment is 10 years And depreciation is assumed to occur at a constant rate every year.

As a result, the efficacy of the continuous light pulse groundwater sterilization system developed as shown in Table 7 was excellent, and it was confirmed that the export to abroad is expected to become active (Table 7).

In order to analyze the discounted cash flow return on investment (ROI), manufacturing expenses and total sales management costs for the production are assumed to be 70% of the yearly sales before replacement, Labor costs, and other utility costs are the same as before the replacement, so it is assumed that there is no change in cost after the introduction of the optical pulse sterilizer. However, due to the optical pulse generator, additional electricity costs are incurred, and the maintenance and repair costs are replaced up to 5 years after the introduction of the optical pulse due to the replacement of xenon lamp, optical pulse sterilization consumable part, It is estimated that it will take about 6,000,000 won per year, doubling from the previous year, and slightly less than 5,000,000 won per year from the 6th year. In addition, it is estimated that R & D expenses will be increased to 4,000,000 won in the first year and 3,000,000 won in the second year due to the application of new technology. The annual profit and BTE were calculated before and after the replacement.

As a result, analysis of discounted cash flow return on investment (ROI) as shown in Table 8 shows that ROI is 32%, which guarantees high profitability.

As described above, according to the optical pulse sterilizer of the embodiment of the present invention, it is possible to control the harmful bacteria and viruses, to easily recognize the data, and to provide the sterilizing effect to the groundwater which is used as food water in the food industry It is possible to provide a continuous optical pulse sterilizer for sterilization of processing water which is more economical than conventional chlorine disinfection systems and ozone sterilizing apparatuses.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation in the embodiment in which said invention is directed. It will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the appended claims.

100: chamber 200: lamp channel
210 to 250: Lamp 300: Inflow pipe
400: discharge pipe 500: power supply part
600: Water pump 700: Controller

Claims (15)

A chamber formed in a hollow shape and extending in a vertical direction;
A lamp channel formed by vertically arranging a plurality of lamps for generating a continuous optical pulse through the chamber;
An inlet pipe connected to a lower portion of the chamber and allowing water to flow into the chamber;
And a discharge pipe connected to an upper portion of the chamber and discharging the water treated by the plurality of lamps.
In claim 1,
Wherein the chamber has a cylindrical shape, a chamber inlet through which the inlet pipe is connected is formed in a lower portion of the chamber, and a chamber outlet through which the outlet pipe is connected is formed in an upper portion of the chamber.
In claim 1,
Wherein the chamber is formed with a window for monitoring water to be treated inside the chamber, the window being formed on top of the lamp channel.
In claim 1,
Wherein a sensor holder on which an energy density measuring sensor is mounted and an air vent through which air in the chamber is discharged are formed on the upper surface of the chamber.
In claim 1,
Wherein the at least two lamp channels are provided and the at least two lamp channels are formed at equal intervals on an outer circumferential surface of the chamber.
In claim 1,
Wherein the optical pulse sterilizing apparatus has four lamp channels, and each lamp channel is formed at equal intervals on the outer circumferential surface of the chamber.
In claim 1,
Wherein the inflow pipe comprises:
A water inlet through which the water flows,
A filter unit for filtering the foreign substances contained in the introduced water;
A flow meter for measuring the flow rate of the introduced water
Wherein the optical pulse sterilizer comprises:
The method according to any one of claims 1 to 7,
The optical pulse sterilizer may include:
A power supply unit for supplying power to the plurality of lamps,
A water pump for allowing the water flowing into the inflow pipe to flow,
A controller for controlling the operation of the lamp channel
Further comprising: an optical pulse sterilizer for generating an optical pulse;
In claim 8,
Wherein the controller includes a trigger circuit capable of simultaneously or sequentially controlling the operation of the plurality of lamps.
In claim 8,
Wherein the controller includes a transmission module capable of transmitting a control signal to the power supply unit and the power supply unit includes a receiving module capable of receiving the control signal and is remotely controlled by the controller. Sterilizing device.
In claim 8,
Wherein the power supply unit includes a field effect transistor (FET), which is a high voltage switch, and controls the duty ratio and the frequency of the continuous optical pulse by the FET.
In claim 8,
Wherein the optical pulse sterilizer has a plurality of lamp channels, and the controller is capable of selectively operating each lamp channel.
In the method for producing laver,
1) sterilizing the working water by using the optical pulse sterilizer of claim 1;
2) removing the impurities using the machining water in step 1); And
3) A method for producing dried kimchi, comprising the step of supplying the kimchi to the molding machine in the form of a mixture of water and deionized water of step 2), and shaping the kimchi in the form of dried laver.
The method of claim 13,
The method according to claim 1, wherein the step (2) is carried out in a size that does not sink when it is introduced into water.
The method of claim 13,
Wherein the step of removing foreign matters in step 2) is carried out two to three times.

Images