KR20160092180A - Method of inositol-assisted early family/individual selection for fast-growing traits in Pinus densiflora - Google Patents

Method of inositol-assisted early family/individual selection for fast-growing traits in Pinus densiflora Download PDF

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KR20160092180A
KR20160092180A KR1020150012483A KR20150012483A KR20160092180A KR 20160092180 A KR20160092180 A KR 20160092180A KR 1020150012483 A KR1020150012483 A KR 1020150012483A KR 20150012483 A KR20150012483 A KR 20150012483A KR 20160092180 A KR20160092180 A KR 20160092180A
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pine
growth
inositol
seedlings
tree
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박응준
이위영
강준원
김현태
최미나
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대한민국(산림청 국립산림과학원장)
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7206—Mass spectrometers interfaced to gas chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0098—Plants or trees

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Abstract

The present invention relates to a method for selecting a fast-growing Pinus densiflora family or individual at an early stage. The method selects a family or individual having a high average inositol content, by measuring the content of inositol extracted from bark tissues including a cambium layer and phloem of a Pinus densiflora seedling during the vigorous growth phase.

Description

[0001] The present invention relates to an inositol-assisted early family / individual selection for fast growing trains in Pinus densiflora,

More particularly, the present invention relates to a method for early selection of a pine tree or a pine tree using the inositol, and more particularly, to a method for early selection of a pine tree, Pinus The present invention relates to a method for early selection of pine trees capable of rapid growth by extracting and analyzing the metabolites of bark, densiflora , and bark by selecting individuals or households having a high content of inositol.

Since the typical growth period of the tree is more than 30 years, it takes a long time to work for the tree breeding. Therefore, it is inefficient because it is less economical than general herb breeding.

In the case of pine trees among the trees, conventional pine tree breeding has become a mainstay of selective breeding that takes a long period of time such as the verification of the growth rate in the mountain area. Recently, accelerated breeding has been proposed to shorten breeding period A Study on the Promotion of Ornamental Formation for Breeding (1988) Proceedings of the Nutrition Society, 31: 433-439), it also takes more than 10 years to select this method.

On the other hand, as a study for early selection of the existing trees with excellent growth, the correlation between the receipt of genetic parameters based on the growth rate, (2007), Journal of the Korean Breeding Society, 39: 457-463).

However, the results of these studies are influenced by various factors such as species characteristics, survey population size, environmental factors, experimental design, and so on.

On the other hand, it has been known that the selection based on the growth characteristics of yuzuchii can increase the heritability by minimizing the factors caused by environmental influences. Especially, the growth of yuzu is generally caused by environmental factors such as moisture and nutrients , But the sequenced growth rate of the family according to genetic characteristics is not affected (Effect of water and nutrient availability in Pinus pinaster Ait. Open pollinated families at an early age: growh, gas exchange and water relations (2006), New Forests, 31: 321-342).

Important factors that can be used to estimate the growth of trees include δ13C rate, nitrogen utilization efficiency, water utilization efficiency and growth hormone content, and early identification of rapid growth individuals using quantitative analysis of these physiological factors (2000) Tree Physiology, 20: 1049-1055). In the present invention, there is provided a method for preparing a compound of formula (I) or a pharmaceutically acceptable salt thereof.

However, since the above-mentioned conventional techniques require a long period of time for breeding of timber, it is urgently required to develop a method capable of early selection because of high consumption of expenses and labor.

The present invention is conceived to solve the problems of the prior art, an object of the present invention is pine (Pinus densiflora ). In particular, the present invention provides a method for early selection of a pine tree or a family tree which can be rapidly grown using inositol among water metabolites.

It is another object of the present invention to provide a method for use as a marker for determining the growth state of a pine seedlings or a mature tree at a certain time.

In order to achieve the above object, the present invention provides a method for measuring inositol content in a dash material extracted from a bark tissue including a forming layer of a pine seedling and a body part, The present invention provides a method for early selection of pine trees with excellent growth, comprising the step of selecting individuals or households having the highest average inositol content,

In addition, the present invention provides a method of using the average inositol content measured in the metabolism material extracted from the bark tissue including the cambium of the pine seedlings and the stem of the pine seedlings as a marker for the growth of the pine tree seedlings do.

In addition, the present invention relates to a method for determining the average amount of inositol measured in the metabolites extracted from the bark tissue including the cambium of the pine seedlings and the stem of the pine seedlings at the time of growing the pine tree, as a marker indicating the peculiar diameter and re- And the like.

According to the present invention, it is expected that improvement of timber productivity in forests can be expected through activation of tree breeding and improvement of quality of breeding species due to shortening of period and cost reduction of long-term tree breeding, bioenery) in the production of fast growing trees that can be used for production.

Fig. 1 shows the correlation between the index of in-situ index and inositol in 34-year-old mature trees in Chuncheon (33 patterns) and Princess (34 patterns).
Fig. 2 shows the relationship between the weight and weight of in-water and inositol in 4-year-old Yoshimitsu (32 bones).

The present invention relates to a method for measuring inositol content in a dash material extracted from a bark tissue including a cambium of a pine seedlings and a body part of a pine seedlings during a period of high pine growth, Or a family member of the pine tree.

In the early selection method of the excellent pine growing method according to the present invention, the period of time during which the growth is vigorous may be from June to August, preferably from July.

In the method for early selection of the pine tree of the present invention, the seedlings may be three to five years old, preferably four years old.

In the early selection method of the present invention, the measurement can be performed using a gas chromatography-mass spectrometer (GC-MS).

In addition, the present invention relates to a method of using the average inositol content measured in the metabolites extracted from the bark tissue including the cambium of the pine seedlings and the stem of the pine seedlings as a marker for the growth of individual pine seedlings will be.

In addition, the present invention relates to a method for determining the average amount of inositol measured in the metabolites extracted from the bark tissue including the cambium of the pine seedlings and the stem of the pine seedlings at the time of growing the pine tree, as a marker indicating the peculiar diameter and re- And a method of using the same.

In the method for use as the marker of the present invention, the period of time during which the growth is vigorous may be from June to August, preferably from July.

In the method of the present invention, the seedlings may be three to five years old, preferably four years old.

In the method of using the markers of the present invention, the mature tree may be a 30- to 35-year-old, preferably a 33- to 35-year-old, more preferably a 34-year-old.

In the method for use as the marker of the present invention, the measurement can be performed using a gas chromatography-mass spectrometer (GC-MS).

The present invention also relates to a pine tree selected early by the early selection method of the above-mentioned excellent pine tree.

Hereinafter, the present invention will be described in more detail.

In order to investigate the growth-related factors by analyzing the contents of individual or intergeneric metabolites, the present invention relates to a method for determining the growth-related factors of the bark tissue including the cambium and stem in the pine seedlings from June to August, And selecting the individuals or households having the highest content of the average inositol among the individuals or households in which the content is measured. The present invention also relates to a method for early selection of pine tree excellent growth individuals or households.

Since tree planting requires a long period of time, it is necessary to develop a method that can be selected early because of the high expenditure of expenses and labor, so it is necessary to determine a proper selection period, In order to raise the effect of improvement, the method of direct selection with the characteristics of yucca neck with high correlation with the characteristics of mature stage and high heritability and the genotype of yucca neck were incomplete in relation with mature stage, There is also an indirect selection method.

On the other hand, among various metabolites of plants, inositol is known as a kind of vitamin, and in particular, myo-inosiotl, which is one kind of inositol, is widely used as a growth promoting substance in tissue culture, and coconut milk It is also a growth promoting substance present in the neutral fraction of malt extract. It is used for the cultivation of many plants, such as callus, for culturing of pear, for growing point, and for drug cultivation.

The present invention relates to the use of the content of inoystole in the bark, including the cambium of the seedling and tubulus of the seedling, in the pine bred breeding group such as the windbreaker and the crossbreeding combination, in June to August, And to provide a method for early selection of pine trees or households that can grow quickly.

The method of early selection of pine trees or households having excellent growth according to the present invention is a retrospective application of the growth of mature trees to the selection of seedlings. Based on the already confirmed mature trees, the mature trees and the three- to five- , Preferably the content of inositol in the 4-year-old pine tree is determined to determine the growth factors that can be used to estimate the growth of the mature tree.

In the method for early selection of pine tree excellent individuals or households according to the present invention, in order to obtain the average value of the individuals or households, it is preferable that at least 10 or more healthy growing 3 to 5 year olds, preferably 4 year olds It is recommended to investigate Yusimoki, and the growth should be a condition that the effect of environmental factors is minimized. It should be excluded from the investigation of growth and inositol content of individuals and repeats that have poor growth due to microenvironmental factors in black forage. Accuracy can be increased.

In order to achieve the object of the present invention, the early selection method of the quick-growing tree using the growth characteristics of the present invention was carried out under various conditions. In order to achieve the object of the present invention, It is desirable to provide a method of early selection of a quick-growing tree using the content of inositol in a bark tissue containing a cambium and a stem.

As an example of a method of using the pine seedlings of the present invention as a marker for showing the growth of the above-ground part, there is a method of selecting the average value of the inositol content of each individual or household by the early selection method of the above- And by comparing the average value of the growth rate (dry weight) of the growing season of the corresponding household in the year.

In addition, the present invention provides a method for producing an extract of the present invention, which comprises extracting a metabolite from bark containing a cambium and a body part in June to August, preferably July, when the pine tree grows most vigorously, Relates to a method of using as a marker indicating the breast diameter and index of rearing of a mature tree of 33 to 35 years old, more preferably 34 year old.

As an example of the method of using the pine tree mature tree according to the present invention as a marker for indicating the diameter and the index of the pine tree, the average value of the inositol content by the household and the pore diameter of the mature tree And the average value of the exponent index.

Hereinafter, the present invention will be described in detail with reference to examples. However, these are for explanation of the present invention in more detail, and the scope of the present invention is not limited thereto.

< Example  1> Stamping  34 years old Mature In the bark  Analysis of correlation between inositol content and growth

Based on the results of the classification of the open-pollinated progeny black forests of 34-year-old trees in Gongju and Chuncheon areas, we divided the households with superior growth and inferior families into two groups , And four families were selected for each

Table 1 shows the result of grouping according to chest diameter, labor, and re-index of each pine tree in the Chuncheon and Gongju areas.

Figure pat00001

Table 2 shows the results of simple correlation analysis between the growth characteristics of the selected 34-year-old, Chuncheon 33 and Prunus 34 pine tree mature trees, and the metabolites extracted from bark tissue containing cambium and tubulus.

Figure pat00002

Fig. 1 shows the correlation between the index of in-situ index and inositol in the 34-year-old mature trees in Chuncheon and Gongju in the two regions.

1, when the correlation between the content of inositol and the index of recruitment was analyzed, the content of inositol in the mature trees of Chuncheon area was found to be the stem volume index (r 2 = 0.303, p < 0.001). In addition, the content of maturein inositol in the princess region showed a significant correlation with the index of the stem volume (r 2 = 0.295, p = 0.001). On the other hand, it can be seen that the group between the good (superior) and bad (inferior, ○) families is clearly distinguished.

< Example  2> 4 year student Seedling In the bark  Analysis of correlation between inositol content and growth

Table 4 shows the average growth rates of the four-year-old seedlings of the present invention and the weights of the weeds.

Figure pat00003

Table 4 shows the results of the simple correlation analysis between the growth characteristics of the four-year-old four-year-old pine trees and the metabolites extracted and extracted from the bark tissue containing the cambium and tubulus.

Figure pat00004

Fig. 2 shows the correlation between the weight and weight of inositol and the weight of the 4-year-old yoshimozu (32 bones).

Referring to Figure 24 Years Old Pine Yushi neck when analyzed the correlation between inositol content and effort and Trunk weight, labor and Trunk weight of each inositol and (r 2 = 0.355, p < 0.001), (r 2 = 0.341, p = 0.002), respectively.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined in the appended claims. And changes may be made without departing from the spirit and scope of the invention.

According to the present invention, it is possible to expect the improvement of the pine productivity in the forest due to the activation of the pine tree breeding and the quality improvement of the reformed species owing to the shortening of the period and the cost reduction of the pine tree breeding which is used for a long period. Can be applied.

Claims (11)

The inositol content in the dash material extracted from the bark tissue including the cambium of the pine seedlings and the body part of the pine seedlings was measured at the time when the growth of the pine was intense and the inositol content in the dash material was measured, A method for early selection of pine trees with excellent growth, including a selection step. The method according to claim 1, wherein the growth period is from June to August. The method according to claim 1, wherein the seedlings are three to five years old. The method of claim 1, wherein the measurement is measured using a gas chromatograph-mass spectrometer (GC-MS). A method of using the average inositol content measured in the metabolic materials extracted from the bark tissue including the cambium and stem of the pine seedlings as a marker of the growth of individual pine seedlings at the height of the pine growth period. A method of using the average inositol content measured in the metabolic materials extracted from the bark tissue including the cambium and stem of the pine seedlings as a marker indicating the peculiar diameter and the index of pine tree size of the pine tree mature trees during the period of vigorous pine growth. The method according to claim 5 or 6, wherein the growth period is from June to August. 6. The method of claim 5, wherein the seedlings are from three to five years old. 7. The method of claim 6, wherein the mature tree is between 30 and 35 years old. The method according to claim 5 or 6, wherein the measurement is measured using a gas chromatograph-mass spectrometer (GC-MS). A pine tree selected by the method of any one of claims 1 to 4.
KR1020150012483A 2015-01-27 2015-01-27 Method of inositol-assisted early family/individual selection for fast-growing traits in Pinus densiflora KR20160092180A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106546461A (en) * 2016-11-07 2017-03-29 赣南师范大学 A kind of Citrus phloem sap preparation method
KR20190136631A (en) * 2018-05-31 2019-12-10 국립생태원 Marker compound for diagnosis of plants withering to death and method of diagnosing growth sate using the same

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106546461A (en) * 2016-11-07 2017-03-29 赣南师范大学 A kind of Citrus phloem sap preparation method
CN106546461B (en) * 2016-11-07 2019-03-22 赣南师范大学 A kind of citrus phloem sap preparation method
KR20190136631A (en) * 2018-05-31 2019-12-10 국립생태원 Marker compound for diagnosis of plants withering to death and method of diagnosing growth sate using the same

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