KR20160063552A - Composition for promoting sleep including hop extract - Google Patents

Abstract

The present invention relates to a food composition for promoting sleep, comprising a humulus extract, and to a pharmaceutical composition for preventing and treating sleep disturbance, comprising a humulus extract.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for accelerating sleep,

The present invention relates to a composition for accelerating sleep surface comprising a hop extract.

Sleep constitutes one-third of human life and is the most fundamental and essential physiological phenomenon and is a very important factor in health maintenance and mental stability. Chronic sleep deprivation and disability negatively impacts overall physical and mental health, including cardiovascular disease, hypertension, memory, learning, metabolic control, weight, immunity, cancer resistance, diabetes, safety accidents and mood. Currently, about 30% of the world's population is suffering from insomnia, and 10% is suffering from chronic sleep disorders.

The lifetime prevalence of insomnia has increased from 36% to 49% over the past several years. This is a serious problem, and according to a joint study conducted by the International Health Organization, the average day for people who can not work during the past month is 4.4 days for people with physical illness, while the average for people with sleep disorders is 5.2 days, The impact of disability on our lives has been very severe. In addition, according to a Gallup survey conducted in the United States in 1995, 52% of adults surveyed reported experiencing drowsiness during the past year, and only three out of ten adults reporting sleep disorders had consulted their physicians about their problems. Even if patients with insomnia complain to their doctor about their symptoms, most doctors have a fragmented knowledge of the disorder, so doctors often use the sleeping pills improperly.

Sleeping drugs are generally effective for short-term treatment of insomnia. However, there are various side effects such as drug dependence and cognitive impairment. Therefore, nonpharmacologic treatment is recommended at the beginning, and severe hypnotics is a common treatment for insomnia. In addition to benzodiazepine, non-benzodiazepine, and benzodiazepine receptor agonists and sedative antidepressants, these drugs have been associated with long-term tolerance and dependence, as well as anxiety, (Waldman, SA, Terzic, A., Pharmacology and Therapeutics, Saunders, pp. 849). In addition, -855, 2009). Therefore, it is urgent to develop a drug that can treat anxiety and insomnia while having fewer side effects.

On the other hand, hop is a vineous perennial plant of Cannabinaceae, and yellow-green flowers are used as beer ingredients. Flowers are also called hops, and there is a small fountain with various kinds of oil and resin. Hope is an important additive of beer. It gives beer specific flavor and bitter taste. It has antiseptic effect, prevents turbidity of protein, improves beer bubbles and increases shelf life. The main components of bitter taste are humulon and lupulon, and the main ingredients of fragrance are humulene and myrcene, which have a preservative effect and a good bubble.

The inventors of the present invention found that the extract of the hop has an effect of accelerating sleep during the study of the efficacy of the hop, further confirmed that the effect of accelerating the sleep is significantly enhanced by fermenting the hop or mixing with the extract of the Snail grass. Completed.

It is an object of the present invention to provide a composition for promoting sleep.

In order to accomplish the above object, the present invention provides a food composition for promoting sleep, comprising a hop extract.

The present invention also provides a pharmaceutical composition for preventing and treating sleep disorder comprising a hop extract.

The food composition and the pharmaceutical composition of the present invention have the effect of improving sleeping time and promoting sleeping.

Fig. 1 shows a state in which EEG rats are measured.
2 shows an electrode insertion operation for EEG measurement of the SD rat.
Figure 3 shows an analysis of the electroencephalogram pattern of SD rats.
Fig. 4 shows a fast Fourier transform (FFT) algorithm for the sleep structure analysis.
Figure 5 shows the total sugars, flavonoids and total polyphenols in the hop extract.
6 shows the Actogram of Drosophila in accordance with the addition of the hop extract.
Column A: Cascade hop extract and control actogram (C: Cascade hop extract administered at 2, 5, and 10 mg with standard samples).
Column B: Hallertau Hop extract and control actogram (H: Hallertau Hop extract 2, 5, 10 mg with standard samples)).
Line C: Saazoff extract and actogram of control (2, 5, 10 mg of Saazoff extract with standard samples)).
Control: Standard samples (5% sucrose and 1% agar).
FIG. 7 shows the difference in the exercise capacity of the fruit flies according to the addition of the hop extract.
Control: Standard samples (5% sucrose and 1% agar),
C 2, 5, 10 mg: Cascade hop extract of standard samples + 2, 5, and 10 mg,
H 2, 5, 10 mg: Hallertau Hof extract of standard samples + 2, 5 and 10 mg,
S 2, 5, 10 mg: Standard sample + 2, 5, 10 mg of SaaShop extract.
Fig. 8 shows changes in the components before and after fermentation of the Cascade hop.
FIG. 9 is an Actogram of Drosophila with addition of Swan grass extract (V: Valerian extract) (Control: Standard sample (5% sucrose and 1% agar), V: 2, 5, 10, 20 mg administered).
Figure 10 shows the locomotor difference of Drosophila as a result of the addition of Rhizosphere Grass Extract (Control: standard sample (5% sucrose and 1% agar), V: Rhizome grass extract with 1, 5, 10, 20 mg).
Figure 11 is an Actogram of Drosophila in the use of a mixture of Hop extract and Swallow grass extract (V: Valerian extract) (Control: Standard sample (5% sucrose and 1% agar), V: Administered with a standard sample, V + C: administration of a mixture of a crude extract (V) and a hop extract (C)
Figure 12 shows the difference in locomotor of the fruit flies when using a mixture of the pre-fermentation and post-fermentation extracts and the ginseng grass extract (Control: standard samples (5% sucrose and 1% agar), Valerian: Administration with a standard sample, administration of a mixture of VA + H: Rhizospheric extract (VA) and cascade hop extract (H)
Figures 13 to 15 show the effect of accelerating sleep in SD rats upon administration of a mixture of extracts of Zygomycetes vulgaris and Hop extract (Figure 13: 1 day of oral administration, Figure 14: 2 days of oral administration, Figure 15: 3 days of oral administration ).
Control: saline group,
VA + H: group administered with a mixture of a crude extract of a mouse (VA) and a Cascade hop extract (H)
VA + FH: a mixture of the extract of Valerium vulgare (VA) and the fermented Cascade hop extract (FH)

The present invention relates to a food composition for promoting sleeping which comprises a hop extract.

The present invention also relates to a food composition for prevention and improvement of sleep disorders comprising a hop extract.

The present invention also relates to a pharmaceutical composition for preventing and treating sleep disorder comprising a hop extract.

Hereinafter, the present invention will be described in detail.

Hope

The hopes of the present invention are commonly used hops. In the present invention, the variety of hops can be used as a single variety or a mixture of two or more varieties. The hop of the present invention may be at least one selected from the group consisting of Cascade, Hallertau and Saaz, and is preferably a cascade.

Hop extract

The hop extract of the present invention may be an organic solvent extract of the hop or an organic solvent extract of the fermentation hop.

The organic solvent may be water or alcohol, preferably water or a lower alcohol having 5 or less carbon atoms, more preferably water, methanol or ethanol, and most preferably ethanol.

The fermentation hopper may be manufactured by inoculating lactobacillus to the hopper and fermenting the same. The lactic acid bacteria may be Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Bifidobacterium and the like, preferably Lactobacillus brevis brevis), but are not limited thereto.

For example, the organic solvent extract of the hop may be prepared by extracting the hop with an organic solvent. The organic solvent extract of the fermentation hopper can be produced, for example, by fermenting the hop with lactic acid bacteria and then extracting the hopper fermented with the lactic acid bacteria with an organic solvent.

Swallow grass extract

The food composition and the pharmaceutical composition of the present invention may further comprise a jujuba grass extract other than the hop extract. The pupa may utilize the roots or rhizomes of the pupa, preferably the roots of the pupa.

The jujuba grass extract is an organic solvent extract of Pyralidae. The organic solvent may be water or alcohol, preferably water or a lower alcohol having 5 or less carbon atoms, more preferably water, methanol or ethanol, and most preferably water.

Composition for accelerating sleep

The present invention relates to a composition for accelerating sleep surface comprising a hop extract. The composition for promoting sleep has the ability to shorten the sleeping time (i.e., sleep inducing ability, sleep latency), ability to enhance sleeping time, or ability to improve sleeping time.

Composition for preventing, ameliorating or treating sleep disorders

The present invention relates to a composition for preventing, ameliorating or treating sleep disorder comprising a hop extract. The above-mentioned sleeping disorder means that the user is suffering from chronic fatigue due to insufficient sleeping or sleeping, or sleeping in the daytime or feeling a headache. The sleeping disorder of the present invention may be insomnia, etc., and also refers to cases where sleeping is not easy or when sleeping, sleeping shallow and unable to take a good night's sleep.

Food composition

The present invention relates to a food composition for accelerating sleep that comprises a hop extract or a hop extract / pine nuts grass extract. The present invention also relates to a food composition for prevention and improvement of sleep disorders, which comprises a hop extract or a hop extract / ginseng grass extract.

The food composition is not limited to a general food, a health supplement, a health functional food, a functional food, an exercise aide, and the like. The hop extract of the present invention or the hop extract / And the like. Preferably, the food composition of the present invention may be a water-surface-inducing food, a sleep-inducing food, a sleep-promoting food, and the like, more preferably a health functional food, a functional food,

The food composition comprising the hop extract of the present invention or the hop extract / paddy grass extract may be used as it is or may be used in combination with other food or food compositions, Lt; / RTI > The amount of the active ingredient to be mixed can be suitably determined according to its use purpose (prevention or health maintenance purpose). In general, the hop extract or hop extract / pine nut extract of the present invention may be added in an amount of 0.01 to 70.00% by weight, preferably 0.01 to 30.00% by weight, based on the raw material, By weight may be added in an amount of 0.01 to 10.00% by weight.

There is no particular limitation on the kind of the food. The food composition comprising the hop extract or the hop extract / pine nut extract may be used in the form of tablets, hard or soft capsules, solutions, suspensions, and the like, which may contain an acceptable conventional carrier, For example, in the case of a preparation for oral administration, it can be prepared using excipients, binders, disintegrants, lubricants, solubilizers, suspending agents, preservatives or extenders.

Examples of the food to which the hop extract or the hop extract / pine nut extract can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, Soups, drinks, tea, drinks, alcoholic beverages, powder formulations and vitamin complexes, but are not limited to these types of foods.

Pharmaceutical composition

The present invention relates to a pharmaceutical composition for prevention and treatment of sleep disorders, which comprises a hop extract or a hop extract / ginseng grass extract. The pharmaceutical composition can be prescribed to a patient suffering from a sleep disorder.

The pharmaceutical composition of the present invention can be administered orally or parenterally and can be used in the form of a general pharmaceutical preparation. The preferred pharmaceutical preparations are those for oral administration such as tablets, hard or soft capsules, liquids, suspensions, etc. These pharmaceutical preparations can be prepared into conventional pharmaceutically acceptable carriers, for example, excipients such as excipients, Binders, disintegrators, lubricants, solubilizers, suspending agents, preservatives or extenders.

The dosage of the pharmaceutical composition of the present invention may be determined by a specialist depending on various factors such as the patient's condition, age, sex, and complications, but is generally from 0.1 mg to 10 g, preferably 10 mg ≪ / RTI > to 1 g. Also, the daily dosage of the pharmaceutical composition per unit dosage form, or a half, 1/3 or 1/4 dose thereof, may be contained, and may be administered 1 to 6 times per day. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of controlling health, the amount may be less than the above range, and the active ingredient may be used in an amount of more than the above range since there is no problem in terms of safety.

BRIEF DESCRIPTION OF THE DRAWINGS The advantages and features of the present invention and the manner of achieving them will become apparent with reference to the embodiments described in detail below with reference to the accompanying drawings. It should be understood, however, that the invention is not limited to the disclosed embodiments, but is capable of many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, To fully disclose the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims.

≪ Materials and methods >

material

Hope uses three varieties of Cascade, Hallertau and Saaz, and the goby paste is purchased from Amazon (Amazone.com). Other analytical reagents were purchased from the market and used more than the first reagent.

Manufacture of Hope Extracts

Hope extracts were refluxed with 70% ethanol using Cascade, Hallertau and Saaz, representative cultivars cultivated all over the world. Specifically, 500 g of 70% ethanol was added to 50 g of the hopper, followed by reflux extraction at 90 ° C. for 4 hours. The reflux extract was centrifuged (2,800 g, 20 min) and the solvent was evaporated using a concentrator. After that, the hop extract was obtained through freeze drying and used in the experiment.

Analysis of components of hop extract

The total sugar content of the hop extract was measured by phenol-sulfuric acid method. The sample was reacted with 5% phenol solution and concentrated sulfuric acid and then analyzed by UV spectrophotometer (TECAN Infinite M200 systems INC., Seestrasse 103 CH-8708 Mannedorf Switzerland) Absorbance was measured at a wavelength of 490 nm and the content of total sugar was calculated from a standard curve formed of glucose as a standard reagent.

The content of flavonoid was measured by DMACA (p-dimethylaminocinnamaldehyde). The DMACA solution was prepared by diluting 0.1% DMACA in methanol containing 1 N HCl. The DMACA solution was prepared by mixing the hop extract and the standard reagent catechin at room temperature for 10 minutes. The standard curve and the content were measured at 640 nm.

The total phenol content was measured by modifying the Folin-Denis method. The sample was mixed with Folin-Ciocalteu's phenol reagent in a 96-well plate, and the mixture was reacted at room temperature for 3 minutes, then saturated sodium carbonate solution was added for 20 minutes, and the absorbance of the reaction solution was measured at 750 nm. The phenol content of the hop extract was calculated from the standard curve of gallic acid prepared with standard reagents.

Hope Fermented  Produce

Lactobacillus brevis M-2 was used as a lactic acid bacterium, and a fermentation product was prepared using a Cascade hop. Lactobacillus brevis M-2 was prepared by inoculating 2% in MRS broth and incubating in a 37 ° C incubator for 18 hours. To prepare the fermentation product of Hope, 100 g of Cascade Hope was collected into a culture container. Thereafter, the cells were autoclaved at 121 ° C for 15 minutes, cooled to room temperature, inoculated with 5% (v / w) L. brevis culture, and then fermented at 37 ° C for 5 days. After completion of the fermentation, 70% ethanol was added in an amount of 5 times (by volume), refluxed and concentrated. The concentrated HOPE extract was lyophilized, pulverized and used as a sample required for the experiment.

Drosophila culture

For the following experiment, the wild type Canton S line provided by Bloomington Drosophila Stock Center (BDSC) was used. Drosophila (8% yeast, 10% sucrose, 5% corn meal and 1% agar) was fed to the juvenile male adult flies in an environment controlled at 24 ° C for 12 hours (12: 12LD) After the stabilization of circadian rhythm in the body for 2 ~ 5 days, the experiment was carried out.

Drosophila Activity Monitor & Locomotor Activity Monitor

To measure locomotor ability, Drosophila Activity Monitor 2 and Locomotor Activity Monitor (TriKinetics) hardware and DAMSystem (TriKinetics) software were used to record the exercise capacity of Drosophila in each group. As a control, Drosophila samples were prepared by adding sucrose-agar media containing 5% sucrose and 1% agar to distilled water. Drosophila samples were prepared by adding hop extracts 2, 5 and 10 mg / Respectively. The principles of Drosophila Activity Monitor (DAM) and Locomotor Activity Monitor (LAM) are as follows. In the DAM system, a set of sensors detects and records how many flies have been moved to the front and back of the tube, one in each of the 32 independent tubes. In order to observe the induction of Drosophila water, a control Drosophila sample and a test Drosophila sample were provided on one side of the tube, and the measurement was performed for 24 hours in a 24 ° C incubator. The light control was set for 24 hours all night, and the changes of circadian rhythm and behavior were observed by the provided samples.

SD Rat  Analysis of sleep structure in model

Day and night cycles (12: 12LD) were automatically maintained in a cage (20 × 26 × 13 cm) for 6 weeks at a temperature of 22-24 ° C and a humidity of 60 ± 5% Electroencephalogram (EEG) measurements were performed after conditioning and adaptation in a controlled laboratory. Experimental animals SD rats were anesthetized with isoflurane (Troikaa Pharmaceuticals Ltd, Gujarat, India) and fixed on a stereotaxic instrument (Fig. 1). After bifurcation of the subcutaneous connective tissues, bregma suture of the exposed skull was confirmed and used as a parenchyma indicator. Microholes were used to position the distal end of the surface electrode on the surface of the cerebral cortex. After bleeding from the puncture site, the electrode was inserted, fixed with dental cement, and the skin was closed (FIG. 2). Surgical site disinfection and antibiotic treatment were performed for 3 days to prevent inflammation caused by surgery, and the behavior, weight, and dietary status were checked for one week of recovery period.

In order to adapt SD rats to the measurement environment, 0.5% saline solution used for the control group was orally administered 4 days before the measurement, and then a recording device was connected to induce compliance with this experiment procedure. Baseline recording Control recording was performed first, and then experimental recording was conducted to measure sleep related functioning. The extracts of the hops and the geranium grass were stabilized for 5 minutes after oral administration, and the electroencephalogram (EEG) was measured for 8 hours from 10:00 to 18:00 using iox2 (emka Technologies, Paris, France) ). The EEG sampling rate was set to 500 Hz and the EEG was set to a filter range of 0.1 to 25 Hz at 15 mm / sec to record the data. The sleep structure analysis was performed by the FFT (fast Fourier transform) algorithm and the ecgAUTO3 program (Ver. 3.3, emka Technologies, Paris, France) was used (Fig. The results were classified into Awake, REM sleep, rapid eye movement, theta band (6-15 Hz), NREM sleep (non-rapid eye movement, delta band: 0.5-3 Hz) . In addition, the movement of the transmitter coupled with the connector of the skull was measured and compared with the electroencephalogram.

Statistical processing

The results obtained from the experiments were analyzed using the statistical program SPSS (SPSS Inc., Chicago, USA). The mean and standard error of three repeated analyzes were calculated. The differences in the general analytical values of the hop extracts were verified by using one-way ANOVA and the Turkey range test at p <0.05 level. Drosophila behavioral analysis and electroencephalographic analysis were performed to determine the p-value for the mean value of the experimental group and the control group as t -test and the significance was verified.

<Experimental Results>

<Experimental Example 1> General analysis of the hop extract

The contents of the general components of the hop extract (i.e., total sugar content, flavonoid, polyphenol content) were measured.

The results are shown in Fig. Specifically, the total content of Cascade hop extract was 163.66 ± 10.96 μg / mL, and the content of Hallertau hop extract and Sazz hop extract was 297.25 ± 2.64 μg / mL and 310.59 ± 14.80 μg / mL, respectively .

The total flavonoid content was also highest in Sazz hop extract (16.26 ± 0.19 μg / mL), followed by Hallertau Hof extract (11.59 ± 0.08 μg / mL) and Cascade hop extract (3.68 ± 0.08 μg / mL) Respectively.

The total polyphenol content was also highest in Sazz hop extract (144.27 ± 0.52 μg / mL), followed by Hallertau Hof extract (104.21 ± 0.49 μg / mL) and Cascade hop extract (75.58 ± 0.05 μg / mL) There was a significantly lower content.

Therefore, total sugar content, total flavonoid and total polyphenol contents of SaaShop extract were the highest.

Experimental Example 2 Evaluation of Sleep Activity of Hop Extract

Measurement of change in Drosophila sleep behavior

Each hop extract was added to sucrose agar (agar) medium at 2, 5, and 10 mg / mL according to the variety of hops to analyze changes in sleep behavior of Drosophila. Actogram J program was used to analyze changes in sleep behavior of the fruit flies. Actograms of the control group and the hop extract group showed changes in the behavior of the fruit flies measured from 24:00 to 22:00, and all the changes in the sleep behavior measured for 5 days were shown. Drosophila showed sleeping from 22:00 to 10:00 with less movement, and it was confirmed that diurnal rhythm was formed through the change of behavior of control flies (Fig. 6). In all of the hop varieties, the behavioral changes at night and day were higher in the Drosophila experimental group receiving 2 mg / mL of the Hope extract than in the control group, and in the Drosophila experimental group consuming the Saaz Hop extract, Showing the greatest change in behavior (Fig. 6). The sum of the Drosophila behaviors at night time was set from 22:00 to 10:00, and the behavior of the Drosophila during the day was graphically set from 10:00 to 22:00. In the Drosophila experimental group receiving the Hope Cascade extract at 10 mg / mL, it was confirmed that the change of the Drosophila behavior at night was significantly less, that is, the 10 mg / mL of the Hope Cascade extract was able to confirm the sleep inducing activity 7).

At daytime, Hallertau Hof extract showed a significantly lower behavioral change at 5 mg / mL. However, the decrease in behavior with sleep inducer intake should be present at night and the decrease in nighttime behavior was significantly different It is difficult to judge that it is contained as a water-inducing substance. The results showed that the extract of Cascade hop contains several kinds of water inducing substances, and the content of Cascade hop extract needs more than 10 mg / mL.

<Experimental Example 3> Analysis of components of fermented product of Cascade hop

In Experimental Example 2, changes in components before and after fermentation of lactic acid bacteria in Cascade hops which were confirmed to have sleep-promoting activity through the fruit fly locomotor test were measured.

As a result, total sugar content was 163.2 μg / mL before fermentation, but decreased to 104.3 μg / mL after fermentation. The content of flavonoids and polyphenols, known as active ingredients, was 16.26 and 144.27 μg / mL before fermentation, And 21.3 and 179.3 μg / mL, respectively (FIG. 8).

Therefore, it was confirmed that the components estimated to be active ingredients increased by fermentation.

&Lt; Experimental Example 4 > Evaluation of sleep-promoting activity of Swallow Pool

The roots of Valerian were extracted with ten times the weight of hot water to obtain a hydrothermal extract of Valerian (hereinafter referred to as Valerian Extract). Then, the watery behavior of Drosophila is analyzed by adding sucrose extract 2, 5, 10, and 20 mg / mL to sucrose medium. For this analysis, the ActogramJ program was used.

The results are shown in Fig. Actograms of control group and jujuba grass extract group showed changes in behavior of fruit flies measured at 24 hours, starting at 22:00, and showed all the changes in sleep behavior measured for 5 days. In the experimental group, the behavior of the fruit flies was different from that of the circadian rhythms due to the unique flavor and taste of the extracts of the Rhesus vulgaris extracts. Respectively. However, it was found that the activity of fruit flies at night and day time was decreased as the content of Extract of Pyranthrene Extract was increased. As a result, it was found that although the amount of rejection was high depending on the flavor and taste, Extended or soothing effect. In particular, at the highest concentration of 20 mg / mL, the sum of the behaviors was significantly reduced both at night and at daytime (FIG. 10).

<Experimental Example 5> Assessment of sleep-promoting activity by use of fermented hop extract

In Experimental Example 2 and Experimental Example 4, the sleep effect on the extracts of hops and pupae was confirmed. Especially, Cascade hop extracts of the hops were superior to those of the Cascade hop extracts. Therefore, in the experiment, Cascade hop lactobacillus fermentations were prepared and used for the improvement of sleep activity. Activity.

<5-1> Estimation of Sleep Enhancement Ability when Mixed with Extract of Hope Extract / Extract of Extract from Grapevine using Drosophila Model

Behavioral changes were analyzed and measured according to the ratio of the hop extracts and the jujuba grass extracts. At this time, the changes of sleep behavior of Drosophila were analyzed by adding sucrose to the medium at 6, 12, 18 mg / mL and 50 mg / mL of extract before and after Cascade Hope fermentation. Respectively.

The results are shown in Fig. Actograms of the experimental group that tested the mixture of the control and the Hope Cascade extract and Rhizome grass extract showed changes in the behavior of Drosophila measured for 24 hours starting at 22:00 and showed all the changes of the sleep behavior measured for 3 days. In the experimental group of Hope Cascade Extract and Rhizopus japonicus extract, the behavior of the first nighttime time did not decrease due to the unique flavor and taste, Respectively.

(P <0.01) in all groups except for the mixture of 18 mg / mL of daytime Hope Cascade extract and 50 mg / mL of Pseudomonas aeruginosa extract on the basis of the sum of nighttime and daytime behavioral changes It was confirmed that the sum decreased.

There was no significant difference in the sum of the behavioral changes at night, but the change of behavior was decreased compared to the control group except the first day, and it was confirmed that the mixture of the two extracts induced the sleeping. In addition, it was confirmed that the water - induced induction effect of the hop extract after fermentation was greater than that of the pre - fermented hops extract. In addition, it was confirmed that the mixture of 12 mg / mL of Hope Cascade extract and 50 mg / mL of Cryptomeria japonica extract before fermentation and after fermentation showed the highest sleep inducing effect. Especially, the addition of hops extract after fermentation showed significant decrease of locomotor activity compared to before fermentation. Therefore, it was confirmed that when the hop extract after fermentation was mixed with the extract of Rhizosphere vulgaris, the sleep-promoting activity was high (FIG. 12).

<5-2> Assessment of Sleep Enhancement Ability when Mixed with Extract of Hope Extract / Extra Extract Using SD rat Model

The water - induced effects of the mixture of 12 mg / mL of Cascade hop extract and 50 mg / mL of P. japonicus extract before and after fermentation were analyzed once again using SD rats. The analysis was performed by performing Electroencephalogram (EEG) measurement using SD rats. The control group was orally administered saline. In the experimental group, a total of 8 hours of EEG was measured from 10:00 to 18:00 by oral administration of a mixture of Cascade hop extract 12 mg / mL and Pyranthrene extract 50 mg / mL before and after fermentation . Experimental animals SD rats were divided into 4 control groups and 4 experimental groups, and oral administration for 3 days was used to measure the improvement of sleeping time and the quality of sleep.

As a result, there was no significant difference in the total sleep time and the awakening time in the experimental group and the control group during the 1 and 2 days of oral administration of the Cascade hop extract 12 mg / mL and the crude extract extract 50 mg / mL, There was no significant difference in the measured NREM sleep and REM sleep (FIGS. 13 and 14).

However, from the second day, NREM sleep, which indicates the quality of sleep, tended to increase. In particular, the addition of the Cascade fermentation hop extract significantly increased NREM sleep (Fig. 14). Total sleep time also tended to increase, but there was no significant difference between the groups.

On the third day of oral administration, the sleeping time was prolonged according to the addition of the hop extract before and after the fermentation. In particular, when the hop extract was added after fermentation, the total sleep time was prolonged when the extract was used alone. However, before fermentation, the total amount of sleeping time seemed to increase, but there was no significant difference between the extracts of Root Extracts alone. However, before and after the fermentation, the extracts of H. japonica showed significantly higher NREM times than those of the extracts alone (Fig. 15).

These results suggest that the use of 12 mg / mL of Hope and 50 mg / mL of Pseudomonas aeruginosa extract tends to increase the sleeping time from day 2. On the third day, the time to take a sleep increased as the NREM increased during the fermentation and after the fermentation, and thus the quality of sleep was increased. In addition, when the fermented hops extract was used together with the extract of Rhizopus spp., The sleep quality increased with the increase of NREM.

Claims (10)

A food composition for promoting sleep, comprising a hop extract.
The method according to claim 1,
Wherein the hop is at least one selected from the group consisting of cascade, hallowth and sauce.
The method according to claim 1,
Wherein the hop extract is a hop alcohol extract.
The method according to claim 1,
Wherein the hop extract is prepared by fermenting a hop with a lactic acid bacterium and then extracting the hopper fermented with the lactic acid bacterium with an organic solvent.
The method according to claim 1,
A food composition for accelerating sleep, characterized by further comprising a pine nut extract.
6. The method of claim 5,
Wherein the gill grass is root or rootstock of the gill grass.
The method according to claim 1,
The food composition for promoting sleep is characterized by having a sleeping time improving ability or a sleeping time improving ability.
A food composition for prevention and improvement of sleep disorders comprising a hop extract.
A pharmaceutical composition for prevention and treatment of sleep disorders comprising a hop extract. 10. The method of claim 9,
A pharmaceutical composition for the prevention and treatment of sleep disorders, which further comprises a crude extract.

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