KR20160051419A - Method For Storage Of Osmia pedicornis for Adult Emergence At Once - Google Patents

Abstract

The present invention relates to a method for storing Osmia pedicornis for simultaneous emergence. The method for storing Osmia pedicornis according to the present invention comprises the following steps: (a) leaving cocoons of Osmia pedicornis at room temperatures for three months; and (b) refrigerating the cocoons from (a) at 0-5°C to enable simultaneous emergence. The method for storing cocoons of Osmia pedicornis of the present invention has a high emergence rate and shortens the time required for emergence, and thus enables simultaneous emergence and provides orchards in pollinating season with Osmia pedicornis as pollinating insects, thereby contributing to increasing the seed setting percentage.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for storing a horned scissors,

More particularly, the present invention relates to a method for storing a horned scissors for temporary alienation, and more particularly, to a method for storing a horned scissors for hibernation, The present invention relates to a method for storing cold storage of Kochi.

Apples are rejuvenated by insects, and bees are the dominant species of pollen-borne insects. However, due to rapid industrialization in recent years, the environment surrounding the apple orchard has been severely changed, and abuse and misuse of pesticides have led to proper application of apples and high- And apple orchards that lack the density of bees required for production. Therefore, the need for pollen-borne insects to replace bees in the apple orchard has been greatly increased.

Osmia spp. And Megachile spp. Of the Hymenoptera bee and Apidae megachilid ae are important pollinator insects in a wide range of pasture cultivation or orchards. And is used worldwide (Bosch, 1994).

Since early 1990s, the horned scallop has been used as a pollen-borne insect in apple orchards. Its pollen-mediating ability to apple flowers is 80 times better than that of bees, and its activity range is concentrated in orchards radiating, It is said that it is relatively easy to manage and multiply, and since the purpose of looking for apple flowers is to collect pollen rather than honey, the contact rate of pistil is as high as 100% compared to 20% of bees, %, Which is higher than 60%.

(1-Holm, 1973; 2-Kristjansson, 1989) and Osmia rufa (L.), Spain (3-Bosch, 1994) and Italy (4-Pinzauti M, 1992; - Ladurner E, 2002) and Serbia (6 - Krunic M, 1995; 7 - Krunic M, 2001) can be referred to as Osmia cornuta (Latreille). In the United States, the American native species, Blue orchard bee and Osmia lignaria, have been used in orchards since the 1970s (8- Torchio, 1976; 9- Bosch and Kemp, 2001) (10- Batra, 1998).

In the 1940s, it was first used in the apple orchard in Aomori, Japan, and in 1940, it was the first country to successfully use it as a pollen-borne insecticide, It is mainly used in the central and northern part of Japan as apples, as well as as a pollen-borne insect of sweet cherries and dried potatoes. Recently, in addition to pears and blueberries, it has also been used in plant crops such as strawberries, (11- Maeta Y, 1978, 12- Maeta Y, 1990, 13- Maeta Y, 2005, 14- Maeta Y, 2006).

In Korea, we introduced horned scissors, which are used in Japanese apple orchards, in Gangdong-ri, Gyungsang-myeon, Chongsong-myeon, Gyeongbuk, Korea in 1992, and began to use them mainly in Gyeongbuk province. After that, (Lee, 2002; Kim, IS, 1999; Lee, 2002). In the early 1990s and early 2000, each apple was distributed nationwide through the Agricultural Technology Center in Zhuzan.

Currently, there are lots of mosquitoes available at Yecheon-gun Insecticide Research Center and Gyeongsangbuk-do Agricultural Resource Service. The amount of pollen-borne insects is increasing each year - Yoon HJ, 2008). However, due to the lack of supply of horned clam due to the breeding problem, and the time of activity and flowering time of the bees are different from each other and the time of their activity is to be controlled, the utilization rate of the horn- , But it is extremely small.

Therefore, there is a need for a method of preserving the horned scissors to orientate the scissors and supply them appropriately at the required time.

1- Holm, S.N., 1973. Osmia rufa L. (Hymenoptera) as a pollinator of plants in greenhouses. Entomol. Scand. 4: 217-224. 2- Kristjanssin K., 1989. Investigations on the possibilities of using solitary bee Osmia rufa L. as a pollinator in Denmark. 146 pp. Doctoral thesis, Department of Crop Science, University Copenhagen, Denmark. 3- Bosch, J., W. Kemp., 2001. How to manage the blue orchard bee as orchard pollinator. Sustainable Agriculture Network National Agricultural Library, Beltsville, MD, USA. 49: 475-482. 4 - Pinzauti M., 1992. Some observations on bio-ethology, flight and foraging activity of Osmia cornuta Latr. (Hymenoptera, Megachilidae) during strawberry pollination. Apicolutura. 8: 7-15. 5-Ladurner E., F. Santi, B. Maccagnani, S. Maini, 2002. Pollination of caged hybrid seed red rape, Brassica oleracea (Brassicaceae), with Osmia cornuta (Latreille) and Apis mellifera L. (Hymenoptera Megachilidae and Apidae ). Bulletin of Insectology. 55: 9-11. 6- Krunic, M., A. Pinzauti, A. Felicioli, Stansavlevicl., 1995. Further observations on Osmia cornuta Latr. and O. lipa L. as alternative fruit pollinators, domestication and utilization. Arch. Biolo. Sci. Belgrade. 47: 59-66. 7- Krunic, M., Lj. Stanisavljevic, M. Brajkovic, Z. Tomanovic, I. Radovic, 2001. Ecological studies of Osmia cornuta (Latr.) (Hymenoptera, Megachilidae) populations in Yugoslavia with special attention to their diapause. Acta. Hortic. 561: 297-301. 8- Torchio P. F., 1976. Use of Osmia lignaria Say (Hymenoptera: Apoidea, Megachilidae) as a pollinator in an apple and prune orchard.- Journal of the Kansas Entomological Society, orchard. J. of the Kansas Entomol. Soc. 49: 475-482. 9- Bosch, J., W. Kemp., 2001. How to manage the blue orchard bee as orchard pollinator. Sustainable Agriculture Network National Agricultural Library, Beltsville, MD, USA. 49: 475-482. 10- Batra, S.W.T., 1998. Horn-faced bees for apple polination. Am. Bee J. 138: 364-365. 11- Maeta, Y. 1978. Comparative studies on the biology of the genus Osmia of Japan. with special reference to their managements for pollination of crops (Hymenoptera: Megachilidae). Bull. Tohoku. Natl. Agric. Exp. Stn. 57: 1-221. 12- Maeta, Y. 1990. Utilization of Wild Bees. Farming Japan. Vol. 24-6: 13-18. 13- Maeta, Y., K. Nadano, T. Kitamura, 2005. Relationship between seed yield of Chinese milk vetch and density of female bees, Osmia cornifrons (RADOSZKOWSKI) (Hymenoptera, Megachilidae). Chugoku Kontyu. 19: 45-61. 14- Maeta, Y., N. Kotaro, F. Katsyra and K. Kenji, 2006. Exploitation of systems to use a univolitine Japanese masin bee, Osmia cornifrons (RADOSZKOWSKI), throughout the year for pollination of greenhouse crop (Hymenoptera, Megachilidae ). Chugoku Kontyu. 20: 1-17. 15- Lee, H. S. and K.S. Woo. 1994. Genus Osmia (Hymenoptera; Megachilidae) from Korea. Korean J. Apicult. 9: 117-130. 16- Kim, I.S. 1999. Studies on the ecological characterstics and utilization of Osmia spp. as pollinators. 50pp. A thesis for the master degree of Kogju National University. 17- Lee, S.W., K.H. Choi, D.H. Lee, D.A. Kim, H.K. Ryu and Y.I. Lee. 2002. Distribution and collection of Osmia bees in the mountain areas of Korea. Korean J. Appl. Entomol. 41: 263-267 18- Yoon H.J., K.Y. Lee, S.B. Lee, I.G. Park, S.J. Jang, Y.C. Choi, Y.S. Choi and G. Lee. 2008. Research on the current status of insect pollinator use in Korea. NAAS, RDA, Korea. Korean J. Apicult. 23: 295-304.

It is an object of the present invention to provide a horn spatula with a horn spatula that is placed at a normal temperature for a certain period of time and then stored at a specified temperature in the range of 0 to 10 DEG C to improve the rate of descent through dormant breaks, And to provide a method for storing the caterpillar catechin.

In order to accomplish the above object, the present invention provides a method for manufacturing a horned beater, comprising: (a) And (b) refrigeration at 0 ° C to 5 ° C.

The present invention also provides a short-term preservation method of horned shear beehive characterized in that the cove of the horned shears is left at room temperature for 6 months and refrigerated for 3 months at 7.5 ° C to 10 ° C.

Since the method of preserving the cocoon of the horned scissors according to the present invention can clearly predict the optimum spinning period and the date and time of the frieze excellent in the abundance rate, it is possible to utilize the horned scissors as an insect at the time required for pollen mediation in the orchard, It is very useful for improving the effective removal rate of fruit in the fruit.

FIG. 1A is a graph comparing the frying rate of female cocoon of the horned scallop frozen at each temperature for 6 months at the natural temperature according to the refrigeration period (female cocoon insect number: 25 per each temperature; weekend).
FIG. 1B is a graph comparing the prevalence rates of the female cocoon of the horned hedgehog refrigerated at the respective temperatures for three months after storage at the natural temperature for each refrigeration period (female cocoon insect number: 20 eggs per temperature; weekend).
FIG. 2A is a graph comparing the prevalence rates of the male cocoon of the horned cheetahs refrigerated at the respective temperatures for 6 months after storage at the natural temperature for each refrigeration period (male cocoon insect number: 75 dogs per temperature; weekend).
FIG. 2B is a graph showing the comparison of the prevalence rates of the male cocoons of the antlers of the antlers, refrigerated at the respective temperatures after being stored for 3 months at the natural temperature, according to the refrigeration period (male cocoon insect number: 80 per each temperature; weekend).
FIG. 3A is a graph comparing the number of days of livestock according to the refrigeration period of the horned scallop female cortex refrigerated at each temperature after being stored at natural temperature for 6 months.
FIG. 3B is a graph comparing the number of days of livestock according to the refrigeration period of the horned scallop female cortex refrigerated at each temperature for 3 months at a natural temperature.
FIG. 4A is a graph comparing the number of days of livestock according to a refrigeration period of a male cortex of a horned cheetah, which is refrigerated at each temperature after being stored at natural temperature for 6 months.
FIG. 4B is a graph showing the comparison of the number of days of cryopreservation by the refrigeration period of the male cocoon, which is refrigerated at each temperature after being stored at natural temperature for 3 months.
FIG. 5A is a graph comparing the rates of descent of the horned scissors and female cocoon for each refrigerator storage container.
FIG. 5B is a graph comparing the rate of descent of the horned scissors and female cocoon for each refrigerated storage container.

Hereinafter, the present invention will be described in detail.

In order to use the horned scallop beetles in the apple orchard, the method of obtaining from pre-existing farms and the method of collecting and securing them outdoors are used. Since the bee's activity time and flowering time are different, , It is necessary to develop a storage method that can clearly understand the period of break of the horned scallop and utilize it effectively for the pollen mediation.

Accordingly, the present inventors confirmed that there is a difference in the abundance rate according to the storage and the period of refrigeration after the natural termination of the horned silkworm cocoon, and ascertained the condition with the highest abundance, and completed the present invention.

As described above, the method of storing the horned scallop can clearly identify the season of the alpinism according to the storage period and the temperature of the refrigerator. Therefore, it is advantageous to appropriately utilize the horned scallop beetle as an insect insect at the time required for pollen mediation in the orchard.

The present invention, in one aspect, comprises the steps of: (a) leaving a cocoon of horned shear at room temperature for 3 months; And (b) storing the cocoon of (a) in a refrigerator at 0 ° C to 5 ° C, wherein the refrigerator storage period is 4 to 9 months .

In another aspect, the present invention includes the step of leaving the cocoon of the horned scissors at room temperature for 3 months, followed by refrigeration for 9 months at 0 캜 to 5 캜. The present invention relates to a method of storing a horned shearbinder, which is characterized in that temporary alienation is possible.

In another aspect of the present invention, there is provided a method for manufacturing a honeycomb structure, comprising the steps of: (a) And (b) refrigerating the cocoons of (a) at a temperature of 7.5 ° C to 10 ° C. The method of the present invention is characterized by the fact that the refrigerator storage period is 3 months do.

In the method for storing the horned beehive of the present invention, the above-mentioned room temperature is 15 to 30 ° C, more preferably, it is in a natural state, and the container for refrigeration is bamboo communication or petri dish, Dish.

Horned scissors occur once a year and appear from late March to April. Usually, males appear first and females appear late to 3 ~ 10 days. After mating, females start pollinating and regeneration activities after 3 ~ 5 days of nutritional intake, especially in apple orchards, mainly from late April to mid May It is known to be active. In addition, it is important to match the maximum activity of the bees with the bloom of the apple blossoms for effective fire prevention activities. For example, in April ~ May when apples and pears are bloomed, the bees appear outside the traffic and take about 5 ~ 7 days The radiation period is known to be 8 to 10 days prior to flowering.

Thus, the present invention is characterized in that, when the horn shears of the present invention are stored in a refrigerator, the number of days of loyalty is within 1 to 5 days. Therefore, it is possible to increase the effective yield rate of the fruit of the fruit farm by irradiating the horned scissors at the correct timing, considering the season of the antlers and the flowering time of the antlers.

The present invention will be described in more detail with reference to the following examples. However, the following examples are provided to aid understanding of the present invention, and the scope of the present invention is not limited by these examples in any sense.

In this case, unless otherwise defined, technical terms and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In the following description and the accompanying drawings, A description of known functions and configurations that may unnecessarily obscure the description of the present invention will be omitted.

[Example]

I. Experimental Method

1. Experimental insects

In order to collect the horned scallop, which is an experimental insect, bamboo communication for collecting horned scallop beetles was installed in the whole country including Sangju, Gyeongsangbuk-do from March 2009 to March 2012, and bamboo communication was collected in the middle of July. Collected horned scimitar beetle The horned scallop was separated from the bamboo flute by a pupa, and female, male pupa and adult were used.

2. Experimental insect management

The horned scissors classified in the middle of July were placed in a natural worm kept at natural temperature, and then weighed by the experimental conditions such as refrigeration period, refrigerated temperature, and refrigerated container.

3. Experimental conditions

 1) Refrigeration time

 In order to investigate the proper period of refrigeration, the horned scissors were divided into two experimental groups: refrigerated for 3, 6 months (natural), and refrigerated for each temperature.

In the natural state, 3 months group was left for 3 months in natural watermelon. Previously, 6 months in the natural state and then refrigerated at 5 ° C was used as a control.

In the experiment, 6 female cocoon, 25 male cocoon, and 75 male cocoon were used in each experimental group for 6 months (control) experiment. In the 3 month experiment, 20 female cocoon and 80 male cocoon were used , And repeated 6 times per test group.

In order to investigate the effects of refrigeration time, we investigated the rate of abstinence, number of days, weight, and longevity. The algebraic rate was calculated by adding the number of adults emerging from the cocoon for 7 days in the experimental insect number. The number of alleged days was calculated by dividing the number of adult emerging from the cocoon in the cocoon for 7 days by the number of adult insects

  2) Refrigeration temperature

In order to investigate the optimal temperature for the horned scissors, we divided the refrigerated temperature into six test groups: 0 ℃, 2.5 ℃, 5 ℃, 7.5 ℃ and 10 ℃. The number of experimental insects was 3 repetitions per test group with 25 female cocoon and 75 male cocoon at each temperature. The contents of the survey were the same as those of the above refrigeration period.

3) Refrigerated storage containers

In order to collect horned scallop beetles, bamboo traffic was installed around March and collected at around July to reduce damage such as rainy season. After being kept at natural temperature as the traffic, it was classified in January and refrigerated at 5 ℃ do. If you leave it in bamboo communication like this, you often get damage (20%) such as nemesis in communication. Therefore, we investigated refrigerated storage containers as a method to increase the survival rate and allegorization rate of horned scissors. The refrigerated container was made of bamboo and petri dish.

The number of experimental insects was 118 in female cocoon, 538 in female cocoon, 114 in female cocoon and 573 in male cocoon for bamboo communication.

II. Experiment result

1. Emergence rate of corkscrew cork

The horned scalloped cocoon was kept at natural temperature for 6 months or 3 months and then stored at 0, 2.5, 5, 7.5 and 10 ℃ for a certain period. As a result, when the cocoons stored at natural temperature for 6 months were refrigerated, they were alprazized at the refrigeration period of 3 months, and at the refrigeration temperature of 7.5 ~ 10 ℃. Therefore, it is advantageous to store the above conditions for short-term storage (Figs. 1A and 2A).

After 3 months of storage at 0, 2.5, 5, 7.5, and 10 ℃, the cocoons stored at natural temperature were fermented, fermented for 9 months, refrigerated at 0 ~ 5 ℃, It is advantageous to store it for a long time (FIG. 1B, FIG. 2B).

2. Days of longevity of corkscrew cork

The horned scalloped cocoon was stored for 6 months and 3 months at natural temperature and then stored at 0, 2.5, 5, 7.5 and 10 ℃ for a certain period.

When the cocoons stored at natural temperature for 6 months were refrigerated and alienated, when female cows were refrigerated for 3 months, fertilization occurred within 4 days to 7 days (Fig. 3A) and male fertilization was observed from 2 days to 6 days ).

When a cocoon stored at a natural temperature for 3 months is refrigerated and alienated, it is alienated within 3 to 5 days when female cocoon is stored for a long period of 9 months (Fig. 3B), and male cocoon is alienated within 2 to 3 days (Fig. 4B). In the case of allegory days, males were more likely to have shorter lived days than females, and as the refrigeration temperature was higher and the refrigeration period was longer (Fig. 3 and Fig. 4).

3. Growth status of horned beetles

As a result of examining the body weights of the horned cauldron adults according to the temperature, period and timing of refrigeration, it was found that the female and male adults, which were not significantly affected by the temperature and period of refrigeration, , And females were found to be heavier than males (Table 1 and Table 2, below).

Changes in body weight during cold storage temperature and period of refrigerated period (3 months storage in natural condition) gender cold storage
term n contrast
(mg) n 0 ℃
(mg) n 2.5 DEG C
(mg) n 5 ℃
(mg) n 7.5 ℃
(mg) n 10 ℃
(mg) Female a 4 months
- - One 85.0 - - One 106.0 One 78.0 2 105.0
ㅁ
7.8 5 months
12 94.5
ㅁ
18.2 2 112.5
ㅁ
16.3 4 106.0
ㅁ
15.0 2 110.5
ㅁ
16.3 7 111.3
ㅁ
17.3 16 105.6
ㅁ
14.8 9 months - - 20 114.5
ㅁ
16.8 20 109.2
ㅁ
15.8 20 105.2
ㅁ
20.7 20 103.8
ㅁ
28.0 - - Average 12 94.5
ㅁ
18.2 23 113.0
ㅁ
17.2 24 108.6
ㅁ
15.4 23 105.7
ㅁ
19.6 28 104.8
ㅁ
25.7 18 105.4
ㅁ
14.1 cock
b 4 months
4 60.3
ㅁ
9.5 9 67.7
ㅁ
6.7 One 66.0 12 65.5
ㅁ
6.9 16 65.1
ㅁ
8.8 - - 5 months
146 64.2
ㅁ
7.7 45 64.4
ㅁ
8.7 55 64.1
ㅁ
7.6 47 62.4
ㅁ
8.4 56 61.5
ㅁ
7.5 180 65.7
ㅁ
8.1 9 months - - 24 67.8
ㅁ
6.7 16 66.9
ㅁ
6.3 10 67.4
ㅁ
8.1 31 67.4
ㅁ
8.2 - - Average 150 64.1
ㅁ
7.8 78 65.8
ㅁ
8.0 72 64.7
ㅁ
7.3 69 63.6
ㅁ
8.3 103 64.0
ㅁ
8.3 180 65.7
ㅁ
8.1

Changes in body weight and temperature of refrigerated temperature of adult horned cauldron (refrigeration period: 6 months in natural condition) gender cold storage
term n contrast
(mg) n 0 ℃
(mg) n 2.5 DEG C
(mg) n 5 ℃
(mg) n 7.5 ℃
(mg) n 10 ℃
(mg) female 2 months
One 84.0 3 92.3
ㅁ
8.5 One 96.0 One 87.0 8 100.0
ㅁ
8.6 13 103.4
ㅁ
18.4 3 months
10 93.5
ㅁ
14.9 16 92.2
ㅁ
17.0 16 96.0
ㅁ
18.5 15 98.3
ㅁ
17.3 17 94.4
ㅁ
21.9 25 94.5
ㅁ
19.9 4 months - - - - 11 98.0
ㅁ
17.8 17 98.6
ㅁ
14.1 14 92.7
ㅁ
16.4 - - Average 11 92.3
ㅁ
14.4 19 92.2
ㅁ
15.8 28 96.8
ㅁ
17.6 33 98.1
ㅁ
15.3 39 95.0
ㅁ
17.9 38 97.6
ㅁ
19.6 cock
2 months
11 62.8
ㅁ
7.1 29 61.6
ㅁ
9.5 24 61.3
ㅁ
8.2 35 62.9
ㅁ
11.4 50 60.5
ㅁ
8.1 45 62.0
ㅁ
10.0 3 months
56 61.6
ㅁ
8.0 49 62.4
ㅁ
9.6 61 62.6
ㅁ
8.1 36 61.3
ㅁ
8.4 43 63.1
ㅁ
11.6 38 63.5
ㅁ
9.0 4 months - - - - 39 62.0
ㅁ
8.2 41 67.4
ㅁ
8.1 31 67.4
ㅁ
8.2 - - Average 67 61.8
ㅁ
7.6 78 62.1
ㅁ
9.5 124 62.1
ㅁ
8.1 112 62.4
ㅁ
9.2 143 61.9
ㅁ
8.4 83 62.7
ㅁ
9.4

4. Survival of horned scissors

As a result of the refrigeration temperature and life span tests, both female and male cocoons were affected by temperature, and the life span of females was 24.3 ㅁ 11.3 days, which was longer than that of males 18.5 ㅁ 8.2 days (Table 3).

 Cold storage temperature and shelf life of adult horned cauldron (refrigeration period: 6 months in natural condition) gender cold storage
term n contrast
(Work) n 0 ℃
(Work) n 2.5 DEG C
(Work) n 5 ℃
(Work) n 7.5 ℃
(Work) n 10 ℃
(Work) Female a One One 10.0 4 30.8
ㅁ
11.3 6 12.3
ㅁ
3.5 4 16.3
ㅁ
3.0 2 30.0
ㅁ
4.2 7 31.1
ㅁ
10.6 2 One 5.0 One 22.0 One 16 - - 2 29.5
ㅁ
13.4 8 13.4
ㅁ
4.6 3 9 26.6
ㅁ
7.6 15 31.5
ㅁ
5.9 16 27.9
ㅁ
6.6 15 18.4
ㅁ
8.9 16 15.6
ㅁ
5.6 20 14.9
ㅁ
8.3 4 - - One 42 11 34.6
ㅁ
8.8 14 35.1
ㅁ
10.6 14 30.1
ㅁ
10.7 2 37.5
ㅁ
4.9 Average 11 23.1
ㅁ
10.3 ab 21 31.4
ㅁ
7.3
a 34 27.0
ㅁ
10.4
ab 33 25.2
ㅁ
12.5
ab 34 23.2
ㅁ
10.9
ab 37 18.9
ㅁ
11.1
b cock
b One - - - - - - - - - - - - 2 9 24.5
ㅁ
1.7 33 21.1
ㅁ
6.8 32 23.4
ㅁ
4.9 45 19.8
ㅁ
5.1 50 18.4
ㅁ
7.2 61 17.3
ㅁ
4.4 3 61 15.6
ㅁ
7.7 55 22.8
ㅁ
8.9 67 19.7
ㅁ
8.9 46 24.4
ㅁ
6.7 59 10.1
ㅁ
3.2 51 11.0
ㅁ
1.4 4 - - - - 53 18.8
ㅁ
3.1 55 11.8
ㅁ
2.1 40 33.1
ㅁ
6.8 - - Average 70 16.8
ㅁ
7.8
CD 88 22.1
ㅁ
8.2
a 152 20.1
ㅁ
6.8
ab 146 18.3
ㅁ
7.2
bc 149 19.0
ㅁ
10.9
bc 112 14.5
ㅁ
4.7
d

5. How to keep refrigerated

As a result of using the existing bamboo communication and alternative storage containers using the Patry Dish in a refrigerator, there was a difference in the frying rate between the female and male cocoons (Fig. 8). Therefore, it is better to use a petri dish that is easy to use (Fig. 5). It is necessary to select the cocoon beforehand and consider the fact that about 20% of the existing bamboo communication is killed by the damage of the natural enemy in communication.

In conclusion, in order to clarify the method of storing cold storage of antlers for temporary allegations, we investigated the rate of abandonment and the number of days of allegiance. For short-term storage, refrigerated storage at 7.5 ~ 10 ℃ in petridish after 6 months of natural storage, After 3 months of natural preservation, it was found appropriate to inactivate the petri dishes at 0 ~ 5 ℃ by refrigerated storage.

Claims (14)

(a) leaving the cocoon of Osmia Pedicornis at room temperature for 3 months; And (b) refrigerating the cocoon of (a) at 0 캜 to 5 캜, wherein temporary alienation is possible.
[2] The method according to claim 1, wherein the refrigerating period is 4 to 9 months.
The method according to claim 1, wherein the room temperature is 15 ° C to 30 ° C.
The method according to claim 1, wherein the container for refrigeration is a petri dish.

The method according to claim 1, wherein the number of days of allegation is one to five days.
(a) leaving the cocoon of Osmia Pedicornis at room temperature for 3 months; And (b) refrigerating the cocoon of (a) at 0 ° C to 5 ° C for 9 months, wherein the temporary alienation is possible.
7. The method according to claim 6, wherein the room temperature is between 15 ° C and 30 ° C.
[7] The method according to claim 6, wherein the container for refrigeration is a petri dish.
7. The method according to claim 6, wherein the number of days of allegation is one to five days.

(a) leaving the crown of the horned scissors for 6 months at room temperature; And (b) refrigerating the cocoon of (a) at a temperature of 7.5 ° C to 10 ° C.
The method according to claim 10, wherein the refrigerating period is three months.
11. The method according to claim 10, wherein the room temperature is between 15 ° C and 30 ° C.
11. The method according to claim 10, wherein the container for refrigerating is Petri dish.
11. The method according to claim 10, wherein the number of days of allegation is one to five days.

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