KR20160035860A - Composition for Controlling Aphid and Method for Controlling Aphid Using the Same - Google Patents

Abstract

Disclosed are a composition for controlling aphids and a controlling method thereof. More specifically, disclosed is a composition for controlling aphids, using a culture fluid of photosynthetic bacteria, lactobacillus, and yeasts which have insecticidal effects against Myzus persicae, Aphis gossypii, and Aphis citricola. Also, disclosed is a controlling method thereof.

Description

TECHNICAL FIELD The present invention relates to a composition for controlling aphid and a method for controlling the same.

The present invention relates to a composition for controlling aphids and a method of controlling the same.

Aphids are insects of the insect river ancestor aphids and generally have a body length of 2 to 4 mm and have a variety of body colors. They collect on the stem, sprout and leaf of various plants and suck the juice of the plant, Most are classified as pests.

Depending on the species, there are some aphids that migrate only a few times during the spawning winter hostage, but only between the aphids and the same host plants as the summer hosts. Peach aphid ( Myzus persicae), cotton aphid (Aphis etc. gossypii), spirea aphid (Aphis citricola) in the electronic and aphids such as pine, beech aphid belongs to the latter.

Peach aphids, cotton aphids, and poppy aphids have a variety of host plants including citrus trees. They succumb to the host plant's juice and cause direct damage or plant viruses, indirectly damaging them.

The use of organic synthetic pesticides has played a pivotal role in the production of food, but the widespread use of organic synthetic pesticides has caused the appearance of insect resistant pests, and the residual toxicity, environmental pollution , And lethal toxicity.

In order to reduce these adverse effects and to meet consumer demand for rapidly growing low-pesticide agricultural products, studies on environmentally friendly control methods are becoming more active.

The present invention discloses a control method using a culture solution of photosynthetic bacteria, lactic acid bacteria and yeast as an environmentally friendly control method for aphids.

It is an object of the present invention to provide a composition for controlling aphid by using a culture medium of photosynthetic bacteria, lactic acid bacteria and yeast.

Another object of the present invention is to provide a method for controlling aphids using the composition for controlling aphids.

The present invention, as identified in the Examples and Experimental Examples below, photosynthetic bacteria, lactic acid bacteria and the culture fluid of yeast peach aphid obtained by the collagen is added to the medium (Myzus persicae), cotton aphid (Aphis gossypii) and spirea with wood aphid (shown a controlling effect on Aphis citricola), the case of the culture GABA is obtained is further added in addition to the collagen particles increases the controlling effects, and the soybean powder when used by adding to the culture medium The antifungal activity Of the total population.

In view of the above, in one aspect, the present invention provides an aphid control composition comprising a photosynthetic bacterium, a lactic acid bacterium, and a yeast culture liquid obtained by the method including the following steps (a) to (c) .

(a) preparing a culture medium for culturing photosynthetic bacteria, lactic acid bacteria and yeast to which collagen particles have been added,

(b) sterilizing the prepared medium, and

(c) culturing the sterilized medium by inoculating a culture medium of photosynthetic bacteria, lactic acid bacteria and yeast,

The photosynthetic bacteria refer to bacteria that perform carbon assimilation using light energy, such as higher plants. These photosynthetic bacteria are chloroblue bacteria sp . ), Chromatium bacterium ( Chromatium sp . ), Rhodospirillum ( Rhodospirillum sp . ), Rhodopsendomonas bacteria ( Rhodopsendomonas sp . ), Rhodobacter sp . ), Rhodopseudomonas bacteria ( Rhodopseudomonas sp . ). The photosynthetic bacteria that can be used in the method of the present invention include Rhodopseudomonas sp . ), Particularly Rhodopseudomonas sp. palustris .

Lactic acid bacteria means microorganisms that decompose saccharides such as glucose to produce lactic acid. In the present specification, lactic acid bacteria are lactic acid bacteria of the genus LactobacillusLactobacillus sp .), Streptococcus lactic acid bacteria (Streptococcus sp .), Lactic acid bacteria in Pediococcus (Pediococcus sp .), Lactobacillus lactic acid bacteria (Leuconostoc sp .) And bifidobacterium lactic acid bacteria (Bifidobacterium sp .). Specifically, the term " lactobacillus < RTI ID = 0.0 >Lactobacillus acidophilus), Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus fermentum (Lactobacillus fermentum), Bifidobacterium longum (Bifidobacterium longum), Bifidobacterium bifidum (Bifidobacterium bifidum) And the like. All of these lactic acid bacteria can be used in the method of the present invention. However, Lactobacillus acidophilus and Lactobacillus plantarum are preferably used in the Examples of the present invention, and Lactobacillus acidophilus and Lactobacillus plantarum are preferably used. If you use it.

Also, any yeast that can be used in the method of the present invention can be used, as long as it is Saccharomyces sp. Thus, the saccharide with my process Lucy (Saccharomyces rouxii), my process celebrity bicyclic by Saccharomyces (Saccharomyces cereviciae), access to the MY Ob pole miss Saccharomyces (Saccharomyces oviformis , Saccharomyces steineri and the like can all be used. Preferably, the case of using saccharomyces cerevisiae used in the following examples is used.

The lactic acid bacteria, yeast and photosynthetic bacteria used in the method of the present invention may be purchased from biotechnology related companies or purchased from domestic or foreign microorganism depository institutions (Biotechnology Research Institute Gene Bank, Korea Microorganism Conservation Center, Korean Agricultural Microbiological Conservation Center, ATCC American Type Culture Collection Associates). If necessary, the strain may be directly used.

The collagen used in the method of the present invention enhances the control effect on aphids when added to the medium as shown in the following experimental examples. The present inventors believe that the effect of this collagen addition is due to the fact that the photosynthetic bacteria, lactic acid bacteria and yeast act as mediators in the formation of colonies, resulting in changes in the characteristics of photosynthetic bacteria, lactic acid bacteria and yeast culture medium (i.e., new microbial metabolites Because it flows out into the culture medium).

Collagen is a protein that constitutes cartilage, tendon, etc. in the body of mammals and is a protein involved in intercellular binding. Such collagen can be used after being inoculated with photosynthetic bacteria, lactic acid bacteria and yeast in a culture medium, and then added to the medium or added at the time of preparing the medium. When added after culturing, it is preferably added about 12 hours after the start of the culture. The amount of collagen added may be in the range of 0.3 to 2.0 parts by weight based on 100 parts by weight of the medium.

The collagen for use in the method of the present invention may be used in powder form, and the smaller the particle size (particle size) of the collagen is, in order to uniformly and easily mix with the medium, the average particle size is usually 0.001 m Lt; / RTI > to 0.0005 mm.

In the method of the present invention, the culture medium for culturing the photosynthetic bacteria, lactic acid bacteria and / or yeast can be prepared containing an energy source, carbon source, nitrogen source, inorganic salt, growth factor and the like suitable for culturing gastric microorganisms have. Energy sources, carbon sources, nitrogen sources, inorganic salts, and growth factors suitable for culturing gastric microorganisms are known in the art. Specifically, Korean Patent Application No. 19950031490, Korean Patent Application No. 1019910018012, Korean Patent Reference can be made to those disclosed in Korean Patent Application No. 1019940001029, Korean Patent Application No. 019990050363, Korean Patent Application No. 1020060049936, Jae-Bum Kim (Jae-Beom Kim, Cultivation of High Concentration Cell Oil of High-Nucleate Yeast Using Industrial Medium, 2001, . Such a medium may be a natural medium using natural materials such as milk, potato, blood, tomato pulverized liquid, rice bran pulverized material, soybean milk, synthetic medium artificially formed of nutrients, and may be a solid medium or a liquid medium. Preferably, natural medium is used for mass culture and economical, and also liquid medium is used.

On the other hand, the culture conditions can be anaerobic or aerobic, and it is possible to cultivate in both anaerobic and aerobic conditions for both photosynthetic bacteria, lactic acid bacteria and yeast. It is known in the art that photosynthetic bacteria are heterotrophic in conditions of cancer culture. The incubation temperature is in the range of 20 ° C to 45 ° C, preferably in the range of 4.0 to 7.5, especially in the range of 6.0 to 7.5. However, anaerobic conditions are preferable considering the growth characteristics of lactic acid bacteria.

In the method of the present invention, the culture method may be a batch culture or a continuous culture.

The sterilization method may be any sterilization method known in the art. In particular, the high-pressure steam sterilization method and the ultraviolet sterilization method used in the embodiment of the present invention are suitable.

In the method of the present invention, it is preferable to add GABA (Gamma-amino butyris acid) to the culture medium and culture.

GABA refers to a neurotransmitter produced from glutamate by glutamate decarboxylase, an amino acid present in the mammalian brain. In addition, GABA reduced blood pressure by activating blood vessel movement (Kayahara H. and Sgiura D., Food & Development., 36 (6): 4-6, 2001) (Ishikawa K. and Saito S., Psychopharmacology, 56: 127-131, 1978; Akaga T., Food & development, 36 (6): 7-9, 2001).

The present inventors believe that this is because Gabala alleviates the stress caused by the formation of colonies between photosynthetic bacteria, lactic acid bacteria and yeast, and consequently changes the characteristics of the culture medium (Ie, the cluster between photosynthetic bacteria, lactic acid bacteria, and yeast is an artificial one that is not formed in a natural state, so that the stress due to the formation of a cluster acts on each other to counteract the tendency to return to a natural state .

The addition of GABA may be carried out at the time of preparing the medium or after the culture. When culturing is performed, it is preferable to inoculate the photosynthetic bacteria, lactic acid bacteria and yeast into the medium and start culturing, and then add them for about 96 hours. The added amount of Gabba may be in the range of 0.1 to 1.5 parts by weight based on 100 parts by weight of the medium.

The composition for controlling aphids of the present invention may preferably further comprise a cheonggukjang powder. The following examples and experimental examples show that the addition of chungkukjang powder significantly improves the control effect against aphids.

Chungkukjang means that the soybeans obtained by fermentation with Bacillus subtilis, the Bacillus subtilis, are added to soybeans as is well known in the art. In the process of fermentation of the added soybean by Bacillus subtilis, a large amount of viscous substances such as lavan form fructan and polyglutamate are produced from soybean saccharide and soy protein by the enzyme produced by Bacillus subtilis, , Phospholipid, isoflavone, phenolic acid, phytic acid, saponin, trypsin inhibitor, tocopherol, unsaturated fatty acid, dietary fiber, oligosaccharide,? -Glutamyltranspeptidase (Γ-glutamyltranspeptidase, γ-GTP), and the like.

In the present invention, the chungkukjang powder can be obtained by drying and pulverizing. The particle size of the chungkukjang powder can be uniformly distributed in the culture medium of photosynthetic bacteria, lactic acid bacteria and yeast. Usually, chungkukjang powder having a particle size of 200 mesh or less is used . The chrysanthemum powder may be contained in the range of 0.1 to 5 parts by weight based on 100 parts by weight of the above-described photosynthetic bacteria, lactic acid bacteria and yeast cultures in the composition for controlling aphids of the present invention.

The composition for controlling aphids of the present invention may contain only the photosynthetic bacteria, lactic acid bacteria and yeast culture liquid as described above, or may further comprise a chrysanthemum powder, and may be an emulsion, a liquid, a suspension, Tablets, emulsions, aerosols, fumigants, and the like.

The composition for controlling aphids of the present invention may be prepared by further containing a carrier commonly used in pest control agents. Examples of the carrier include solid carriers, liquid carriers, gaseous carriers, surfactants and dispersants. Examples of the liquid carrier include methanol, n-hexanol, ethylene glycol, acetone, methyl ethyl ketone, cyclohexanone, n-hexyl alcohol,

The reaction product may be used in the presence of an acid, a kerosine, a kerosene, a toluene, a xylene, a methylnaphthalene, a diethylether, dioxane, tetrahydrofuran, ethyl acetate, acetonitrile, isobutyronitrile, dimethylformamide, dimethylacetamide, Seed, water and the like. Examples of the gas phase carrier include LPG, air, nitrogen, carbon dioxide, dimethyl ether and the like.

Examples of the surfactant or dispersant for emulsification, dispersion, electrodeposition, etc. include alkylsulfonic acid esters, alkyl (aryl) sulfonic acid salts, polyoxyalkylene alkyl (aryl) ethers, polyhydric alcohol esters and ligninsulfonic acid salts have. If necessary, carboxymethyl cellulose, gum arabic, polyethylene glycol, calcium stearate and the like may be used as adjuvants for improving the properties of the preparation.

The carrier, surfactant, dispersant, adjuvant and the like may be included in the aphid control composition of the present invention, alone or in combination, according to the characteristics of the formulation, the purpose of pest control, the target pest to be applied, and the like.

 Meanwhile, the photosynthetic bacteria, lactic acid bacteria, and yeast culture solutions, which are effective ingredients in the composition for controlling aphid disease of the present invention, are contained in the range of 0.1 to 99.9% by weight based on the total weight of the composition, May be included in any amount.

In another aspect, the present invention relates to a method of controlling aphids using a composition for controlling aphids as described above.

The aphid control method of the present invention comprises the step of contacting aphid control composition with aphids.

The contacting step may be performed by spraying or applying the composition for controlling aphids directly to a plant inhabited by aphids, or by spraying, injecting or mixing the soil in a habitat environment of a plant. Preferably, the aphid is sprayed or applied directly to a plant inhabited by aphids.

As described above, according to the present invention, there can be provided an environmentally friendly composition for controlling aphids using a culture solution of photosynthetic bacteria, lactic acid bacteria and yeast, and a method for controlling aphids using the composition.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Preparation of aphid control composition

Example 1 Preparation Example 1 of aphid control composition

The medium was 95 liters of water, 0.05 kg of crushed crab shells, (0.03 kg), 0.02 kg of ground beef, 0.02 kg of ground beef, 0.02 kg of ground beef, 0.02 kg of ground beef, 0.02 kg of ground beef, 3.24 L of molasses, 1.5 L of milk and 0.5 kg of collagen powder were mixed uniformly.

Next, the prepared culture medium was sterilized at a sterilization temperature of 121 占 폚 for about 20 minutes, and then the medium was placed in a fermentation container having a capacity of about 15 L, and the cultured Lactobacillus plantarum ), yeast ( Saccaromyces cerevisiae ) and photosynthetic bacteria ( Rhodopseudomonas The culture medium to be inoculated with each of 2% (v / v) of palustris) and the culture temperature 30 ℃, pH 6.5 conditions and the anaerobic conditions and cultured for about 16 days ash.

The culture solution was directly used as an aphid control composition in the following experiment.

&Lt; Example 2 > Preparation Example 2 of aphid control composition

The aphid control composition was prepared under the same process conditions as in <Example 1> except that Lactobacillus plants ( Lactobacillus plantarum ) instead of Lactobacillus acidophilus ( Lactobacillus acidophilus ) was used.

Example 3 Preparation Example 3 of aphid control composition

The aphid control composition was prepared under the same process conditions as in Example 1 except that 0.3 kg of GABA (Gamma Amino Butyric Acid) was added and cultured 4 days after the initiation of cultivation.

Example 4 Preparation Example 4 of aphid control composition

The aphid control composition was prepared under the same process conditions as the above <Example 2> (that is, lactic acid bacteria were Lactobacillus acidophilus ), but after 4 days from the start of cultivation, 0.3 kg of GABA (Gamma Amino Butyric Acid) was added and cultured.

Example 5 Preparation Example 5 of aphid control composition

The culture solution obtained in Example 1 and chungkukjang powder were mixed at a weight ratio of 500: 1 (culture: chungkukjang powder) to prepare aphid control composition.

Example 6 Preparation Example 6 of aphid control composition

The culture solution obtained in Example 2 and chungkukjang powder were mixed at a weight ratio of 500: 1 (culture: chungkukjang powder) to prepare aphids control composition.

Example 7 Preparation Example 7 of aphid control composition

The culture solution obtained in Example 3 and the chungkukjang powder were mixed at a weight ratio of 500: 1 (culture: chungkukjang powder) to prepare aphids control composition.

Example 8 Preparation Example 8 of aphid control composition

The culture obtained in Example 4 and the chungkukjang powder were mixed at a weight ratio of 500: 1 (culture: chungkukjang powder) to prepare aphids control composition.

< Experimental Example > Control effect on aphids

&Lt; Experimental Example 1 > Effect of controlling the peach aphid

Effect of peach aphid control The test was carried out on 3.0 × 3.0 cm sized Chinese cabbage leaves with peach aphid ( Myzus persicae ) larvae were inoculated with a soft brush, and the control composition of the above example was sprayed and sprayed three days later, and the control ratio was calculated according to the following formula. All experiments were performed three times.

The aphid control agent of the comparative example was prepared in the same manner as in Example 1 except that a culture medium of photosynthetic bacteria, lactic acid bacteria and yeast was prepared and the culture was prepared using a culture medium to which no collagen was added (Comparative Example 1) Of water (Comparative Example 2) was used.

Control rate (%) = (untreated raw embryo - treated raw embryo) / untreated raw embryo × 100

The results are shown in Table 1 below.

Control rate (%) division Control rate (%) Example 1 36.8 ± 5.6 Example 2 38.4 ± 4.7 Example 3 56.2 ± 8.2 Example 4 55.5 ± 7.3 Example 5 62.7 ± 3.7 Example 6 64.2 ± 6.5 Example 7 87.2 ± 5.8 Example 8 84.6 ± 3.9 Comparative Example 1 14.2 ± 4.2 Comparative Example 2 7.5 ± 2.6

[Table 1] shows the effect of controlling the culture itself. However, it shows that the control effect is enhanced when the culture is carried out with the addition of GABA, and the control effect is enhanced when the culture is performed by adding the chungkukjang powder. For reference, the culture solution or the chungkukjang powder itself obtained by using the culture medium without collagen did not exhibit any special controlling effect.

<Experimental Example 2> Effect of controlling the cotton aphid

Antifungal activity test for cotton aphid (Aphis gossypii) are were used for seedling assay, specifically, and by pre-inoculated with aphid on cucumber seedlings sprayed after the embodiment of the sample subjected to the settled aphids on seedling leaves pre-irradiated prior example sample processing spray After 3 days, the control rate was investigated according to the following formula. All experiments were performed three times.

Control rate (%) = (untreated raw embryo - treated raw embryo) / untreated raw embryo × 100

The results are shown in Table 2 below.

Control rate (%) division Control rate (%) Example 1 27.4 ± 3.9 Example 2 24.6 ± 6.2 Example 3 47.6 ± 5.4 Example 4 43.6 ± 4.7 Example 5 58.4 ± 6.2 Example 6 62.7 ± 3.6 Example 7 79.4 ± 6.2 Example 8 83.2 ± 4.8 Comparative Example 1 11.5 ± 2.8 Comparative Example 2 2.4 ± 1.6

[Table 2] shows a similar tendency to the results of Table 1 for the peach aphid, and shows that the control effect is enhanced when added with Gaba, and the control effect is enhanced when the chungkukjang powder is added.

&Lt; Experimental Example 3 > Control effect on aphids

The control effect of Aphis citricola was tested in a manner similar to that of Experimental Example 1 above. Specifically, the control of the control composition of the above example was sprayed with 20 brushstroke aphids by a soft brush, and the control ratio was calculated according to the following equation after three days. All experiments were performed three times.

Control rate (%) = (untreated raw embryo - treated raw embryo) / untreated raw embryo × 100

The results are shown in Table 3 below.

Control rate (%) division Control rate (%) Example 1 32.4 ± 6.2 Example 2 26.5 ± 6.5 Example 3 49.7 ± 4.2 Example 4 44.2 ± 5.1 Example 5 68.9 ± 7.3 Example 6 61.5 ± 6.1 Example 7 82.8 ± 3.2 Example 8 84.7 ± 7.3 Comparative Example 1 9.4 ± 7.1 Comparative Example 2 4.3 ± 3.1

The results of the above [Table 3] are also similar to those of [Table 1] and [Table 2], indicating that the addition of Gabba increases the control effect and the control effect is enhanced when the chungkukjang powder is added.

Claims (7)

An aphid control agent composition comprising a photosynthetic bacterium, a lactic acid bacterium, and a culture of yeast obtained by a method comprising the following steps (a) to (c) as an active ingredient.
(a) preparing a culture medium for culturing photosynthetic bacteria, lactic acid bacteria and yeast to which collagen particles have been added,
(b) sterilizing the prepared medium, and
(c) culturing the sterilized medium by inoculating a culture medium of photosynthetic bacteria, lactic acid bacteria and yeast,
The method according to claim 1,
The photosynthetic bacteria are Rhodopseudomonas palustris ,
The lactic acid bacteria are lactobacillus acidophilus or lactobacillus flutarium,
Wherein the yeast is Saccharomyces cerevisiae. &Lt; RTI ID = 0.0 &gt; 8. &lt; / RTI &gt;
The method according to claim 1,
The aphid is a peach aphid ( Myzus persicae), cotton aphid (Aphis gossypii ) or Aphis citricola . &lt; / RTI &gt;
The method according to claim 1,
Wherein the ghava is added to the medium of step (a) or added during the culturing step of step (c).
The method according to claim 1,
Wherein the composition further comprises a cheonggukjang powder.
6. The method of claim 5,
Wherein the chrysanthemum powder is contained in an amount ranging from 0.1 part by weight to 5 parts by weight based on 100 parts by weight of the culture solution.
A method of controlling aphids, comprising contacting the aphid control composition according to any one of claims 1 to 6 with aphids.