KR20160020766A - Multi-measuring apparatus of absorbance for realtime molecular diagnosis - Google Patents
Multi-measuring apparatus of absorbance for realtime molecular diagnosis Download PDFInfo
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Abstract
The present invention provides a real-time molecular mass spectrometer for molecular diagnostics, which comprises a plurality of light emitting diodes for emitting light to a plurality of samples, transmitting the transmitted light through an optical fiber to an image sensor, Multiple measurements are possible. By irradiating light in the longitudinal direction of the reaction sample using a tubular reaction vessel, it is possible to maximize the change of absorbance by lengthening the optical path, and by irradiating light to the side using a rectangular reaction vessel, The absorbance at a high sensitivity can be measured. Also, since the reflector is provided on the bottom surface of the flat tubular reaction vessel and on one side of the rectangular parallelepiped, light is irradiated from the light emitting diode to the reflector, and the absorbance can be measured by lengthening the optical path by total reflection occurring in the reflector.
Description
The present invention relates to an absorbance measuring apparatus, and more particularly, to an apparatus for irradiating a plurality of samples with light and multiplexing the absorbed light of the sample in real time using an image sensor.
Molecular diagnostics can be used for the detection of hereditary diseases, identification of genetic fingerprints, diagnosis of infectious diseases, cloning of genes, paternity testing, And DNA computing are widely used for analysis and diagnosis purposes.
Among them, the PCR (Polymerase Chain Reaction) device is developing an efficient method for real-time monitoring of the PCR process as well as efforts to improve the PCR yield. Real-time PCR is a real-time PCR system that integrates a temperature controller and a spectrophotometer. The fluorescence material is injected into the PCR chamber, A technique for measuring an optical signal generated by coupling with an amplification product is used. However, this requires the use of different fluorescent pigments in the spectrum for each assay in a multiplex reaction, and also requires a separate light source module to activate the optical signal from the fluorescent material, an optical signal obtained from the amplified nucleic acid A light detection module for controlling the light path, and a reflector for adjusting the light path.
Korean Patent Laid-Open Publication No. 2013-0113072 relates to an absorbance measurement device, and relates to an absorbance measurement device capable of sensing an absorbance state of a sample injected into a well using a CMOS image sensor. However, it is impossible to measure relatively precisely because the optical path is short using the well plate.
Korean Patent Laid-Open Publication No. 2004-0048754 relates to a fluorescence detection apparatus and discloses an apparatus for searching and analyzing temperature-sensitive biological actions in real time using the change in luminous intensity of fluorescence. However, this requires the use of a fluorescent dye by using a fluorescence search, and it requires a filter, an additional lens, and the like to exhibit a complicated structure.
Therefore, there is a need for an absorbance measurement device capable of measuring a large number of samples, controlling the temperature and simplifying the structure, and accurately measuring in real time.
Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide an apparatus for irradiating a plurality of samples with light and multiplexing the transmitted light with high sensitivity in real time using an image sensor.
DISCLOSURE OF THE INVENTION In order to solve the above problems, the inventors of the present invention have discovered that by extending the path of light transmitted through a sample to measure a precise absorbance, and by using an optical fiber and an image sensor, the structure of the apparatus is simplified, and the present invention has been accomplished.
The present invention relates to an optical absorption multiple measurement apparatus for real-time molecular diagnosis, the apparatus comprising: a plurality of tubular reaction vessels; A heating block having a plurality of tubular reaction vessels spaced apart from each other and having a plurality of channels penetrating at both longitudinal ends so that heat can be transferred to the side of the tubular reaction vessel; A plurality of light emitting diodes (LEDs) positioned above each of the plurality of tubular reaction vessels and irradiating light in the longitudinal direction of the respective tubular reaction vessels; A plurality of optical fibers having one end located at the bottom of each of the plurality of tubular reaction vessels and the other end located at the top of the image sensor; An image sensor which is irradiated from the plurality of light emitting diodes and simultaneously detects light passing through the plurality of tubular reaction vessels and the optical fiber; A display connected to the image sensor and displaying an image from the image sensor; And a storage unit for storing the image at a predetermined time interval.
The present invention also relates to an apparatus for multi-spectrophotometry for molecular diagnostics in real time, the apparatus comprising: a plurality of tubular reaction vessels flat on the underside; A reflector which is in contact with the inside or outside of the bottom of the reaction vessel toward the inside of the tubular reaction vessel; A heating block having a plurality of tubular reaction vessels in contact with the reflector spaced apart from each other and having a plurality of channels penetrating both longitudinal ends so that heat can be transferred to the longitudinal side of the tubular reaction vessel, ; A plurality of light emitting diodes (LEDs) positioned above each of the plurality of tubular reaction vessels and irradiating light toward the reflectors of the respective tubular reaction vessels at a predetermined angle; A plurality of optical fibers having one end located at the top of each of the plurality of tubular reaction vessels and the other end located at the top of the image sensor; An image sensor that is irradiated from the plurality of light emitting diodes, passes through the plurality of tubular reaction vessels, is reflected by a reflector on the bottom of the reaction vessel, and simultaneously detects light passing through the optical fiber; A display connected to the image sensor and displaying an image from the image sensor; And a storage unit for storing the image at a predetermined time interval.
The present invention is also a real time molecular mass spectrometry apparatus for real time molecular diagnostics, comprising: a plurality of rectangular parallelepiped reaction vessels; A heating block having a plurality of rectangular parallelepiped reaction vessels spaced apart from each other and having a plurality of channels penetrating both sides in a transverse direction so that heat can be transferred to both sides and a lower portion of the rectangular parallelepiped reaction vessel; A plurality of light emitting diodes (LEDs) positioned at side surfaces of each of the plurality of rectangular parallelepiped reaction vessels and irradiating light to one side of the rectangular parallelepiped reaction vessel having the longest optical coupling distance; A plurality of optical fibers having one end disposed on one side of the plurality of rectangular parallelepiped reaction containers facing the light emitting diode (LED) and disposed opposite to the light emitting diode, and the other end positioned on the image sensor; An image sensor which is irradiated from the plurality of light emitting diodes and simultaneously detects light passing through the plurality of tubular reaction vessels and the optical fiber; A display connected to the image sensor and displaying an image from the image sensor; And a storage unit for storing the image at a predetermined time interval.
The present invention is also a real time molecular mass spectrometry apparatus for real time molecular diagnostics, comprising: a plurality of rectangular parallelepiped reaction vessels; A reflector which is in contact with the inside or outside of the one face of the reaction vessel toward the interior of the rectangular parallelepiped reaction vessel; A heating block having a plurality of rectangular parallelepiped reaction vessels to which the reflector is in contact and spaced apart from each other and having a plurality of channels penetrating both sides in a lateral direction so that heat can be transferred to both sides and a lower portion of the rectangular parallelepiped reaction vessel, ); A plurality of light emitting diodes (LEDs) positioned on opposite sides of the reflector of each of the plurality of rectangular parallelepiped reaction vessels and irradiating light toward the reflector at a predetermined angle; A plurality of optical fibers positioned at one end of each of the plurality of rectangular parallelepiped reaction vessels on the opposite side of the reflector and the other end of the optical fiber positioned above the image sensor; An image sensor which is irradiated from the plurality of light emitting diodes, passes through the plurality of rectangular parallelepiped reaction vessels, is reflected by a reflector on the opposite side, and simultaneously detects light passing through the optical fiber; A display connected to the image sensor and displaying an image from the image sensor; And a storage unit for storing the image at a predetermined time interval.
The present invention also provides a real time molecular mass spectrometer for absorbance measurement in which the tubular reaction vessel has a cylindrical shape, a square columnar shape, a triangular columnar shape, and an inverted conical shape.
The present invention also provides a real time molecular mass spectrometer for measuring extinction of absorbance at a ratio of length to width to height of from 1: 5: 1 to 1: 10: 5.
The present invention also provides a real-time molecular mass spectrometer for real time molecular diagnostics, wherein the predetermined angle is an incident angle of light emitted from a plurality of light emitting diodes, and the incident angle is 1 to 10 degrees.
The present invention also provides a real time molecular mass spectrometer for absorbance measurement, wherein the image sensor is CCD or CMOS.
The present invention also provides a real time molecular mass spectrometer for absorbance measurement, wherein the reaction of the reaction vessel is a PCR or LAMP reaction.
In the real time molecular diagnostics absorbance meter for real-time molecular diagnostics, light is radiated in the longitudinal direction of a tubular reaction vessel using a light emitting diode, thereby maximizing the sensitivity to the change in absorbance as the optical path is lengthened. There are advantages of simplifying the equipment by using sensor.
BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a schematic diagram showing one unit of an apparatus using a tubular reaction vessel according to one embodiment of the present invention.
FIG. 2 is a schematic diagram showing the configuration of an apparatus for measuring the absorbance of the real time molecular diagnostics according to an embodiment of the present invention.
3 is a schematic view showing a unit of an apparatus using a reaction vessel equipped with a reflector according to an embodiment of the present invention.
4 is a schematic diagram showing one unit of an apparatus using a rectangular parallelepiped reaction vessel according to one embodiment of the present invention.
In one embodiment, the present invention is a real time molecular mass spectrometry apparatus for multi-spectrophotometry, the apparatus comprising: a plurality of tubular reaction vessels; A heating block having a plurality of tubular reaction vessels spaced apart from each other and having a plurality of channels penetrating at both longitudinal ends so that heat can be transferred to the side of the tubular reaction vessel; A plurality of light emitting diodes (LEDs) positioned above each of the plurality of tubular reaction vessels and irradiating light in the longitudinal direction of the respective tubular reaction vessels; A plurality of optical fibers having one end located at the bottom of each of the plurality of tubular reaction vessels and the other end located at the top of the image sensor; An image sensor which is irradiated from the plurality of light emitting diodes and simultaneously detects light passing through the plurality of tubular reaction vessels and the optical fiber; A display connected to the image sensor and displaying an image from the image sensor; And a storage unit for storing the images at predetermined time intervals.
When the transmitted light intensity of the order of a solvent and a solution of the same thickness as the quantity representing the degree of the absorbing solution in the absorption column of the present invention, respectively referred to I 0, I is defined as log 10 (I 0 / I) . In broad terms, I 0 is the intensity of incident light, and I is the intensity of light transmitted through gas, liquid, or solid material. In the case of Lambert-Beer's law, log 10 (I 0 / I) = ε cd (where ε is the molecular extinction coefficient, c is the molar concentration and d is the thickness of the absorption layer) The concentration of the substance can be measured from the absorbance based on the principle that the length of the optical path is proportional to the area of the concentration.
The optical density (OD) of the present invention is a measure of the degree of transmission of light through the inside of a material or reflection on the surface of the material. Light passing through the water to such an extent that the water becomes turbid due to suspended substances in the water, In the present invention, the result of the Lambert-Beer law is defined as the absorbance, the value divided by the thickness is defined as the optical density, and the absorbance includes the optical density.
In the present invention, for example, the biological molecule sample is a sample used in a PCR (Polymerase Chain Reaction) or LAMP (Loop-mediated isothermal amplification) reaction in an embodiment in which the absorbance of a biological molecule sample is measured, Of the absorbance of the sample.
BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a schematic diagram illustrating the path of light as a unit of an apparatus using a tubular reaction vessel in accordance with an embodiment of the present invention. The
2 schematically shows the configuration of the absorbance measurement apparatus of the present invention. The apparatus includes a plurality of
The absorbance of the tubular reaction container according to the present invention can be measured by irradiating light in the longitudinal direction of the tubular reaction container and expanding the optical path. Therefore, in another aspect, the present invention is a real-time molecular mass spectrometer for absorbance measurement using a reflector for expanding an optical path. The apparatus comprises: a plurality of tubular reaction vessels having a flat bottom; A reflector that is in contact with the inside or outside of the bottom of the reaction vessel toward the top of the tubular reaction vessel; A heating block having a plurality of tubular reaction vessels in contact with the reflector spaced apart from each other and having a plurality of channels penetrating both longitudinal ends so that heat can be transferred to the longitudinal side of the tubular reaction vessel, ; A plurality of light emitting diodes (LEDs) positioned above each of the plurality of tubular reaction vessels and irradiating light toward the reflectors of the respective tubular reaction vessels at a predetermined angle; A plurality of optical fibers having one end located on top of each of the plurality of tubular reaction vessels and the other end directed toward the image sensor; An image sensor that is irradiated from the plurality of light emitting diodes, passes through the plurality of tubular reaction vessels, is reflected by a reflector on the bottom of the reaction vessel, and simultaneously detects light passing through the optical fiber; A display connected to the image sensor and displaying an image from the image sensor; And a storage unit for storing the images at predetermined time intervals.
Fig. 3 is a unit of the absorbance measurement apparatus using the reflector of the present invention. Each tubular reaction vessel in the apparatus is a
The
In another aspect, the present invention is a real-time molecular mass spectrometer for absorbance measurement with different irradiation directions. The apparatus includes a plurality of rectangular parallelepiped reaction vessels; A heating block having a plurality of rectangular parallelepiped reaction vessels spaced apart from each other and having a plurality of channels penetrating both sides in a transverse direction so that heat can be transferred to both sides and a lower portion of the rectangular parallelepiped reaction vessel; A plurality of light emitting diodes (LEDs) positioned at side surfaces of each of the plurality of rectangular parallelepiped reaction vessels and irradiating light to one side of the rectangular parallelepiped reaction vessel having the longest optical coupling distance; A plurality of optical fibers having one end disposed on one side of the plurality of rectangular parallelepiped reaction containers facing the light emitting diode (LED) and disposed opposite to the light emitting diode, and the other end positioned on the image sensor; An image sensor which is irradiated from the plurality of light emitting diodes and simultaneously detects light passing through the plurality of tubular reaction vessels and the optical fiber; A display connected to the image sensor and displaying an image from the image sensor; And a storage unit for storing the images at predetermined time intervals.
4 is a schematic diagram showing one unit of an apparatus using a rectangular parallelepiped reaction vessel according to one embodiment of the present invention. The rectangular
The present invention is also a real-time molecular mass spectrometer for absorbance measurement with a reflector on one side of a rectangular parallelepiped reaction container. The apparatus includes a plurality of rectangular parallelepiped reaction vessels; A reflector which is in contact with the inside or outside of the one face of the reaction vessel toward the interior of the rectangular parallelepiped reaction vessel; A heating block having a plurality of rectangular parallelepiped reaction vessels to which the reflector is in contact and spaced apart from each other and having a plurality of channels penetrating both sides in a lateral direction so that heat can be transferred to both sides and a lower portion of the rectangular parallelepiped reaction vessel, ); A plurality of light emitting diodes (LEDs) positioned on opposite sides of the reflector of each of the plurality of rectangular parallelepiped reaction vessels and irradiating light toward the reflector at a predetermined angle; A plurality of optical fibers positioned at one end of each of the plurality of rectangular parallelepiped reaction vessels on the opposite side of the reflector and the other end of the optical fiber positioned above the image sensor; An image sensor which is irradiated from the plurality of light emitting diodes, passes through the plurality of rectangular parallelepiped reaction vessels, is reflected by a reflector on the opposite side, and simultaneously detects light passing through the optical fiber; A display connected to the image sensor and displaying an image from the image sensor; And a storage unit for storing the images at predetermined time intervals.
In one embodiment of the present invention, the rectangular parallelepiped reaction container has a ratio of width to height of 1: 5: 1 to 1:10: 5. In one embodiment of the present invention, the total angle of incidence of the light irradiated to the reflector in the plurality of light emitting diodes is 1 deg. To 10 deg., And total reflection occurs in the reflector in contact with one side surface of the rectangular parallelepiped reaction vessel. The light reflected from the reflector passes through the reaction solution once more in the direction from the bottom to the top, and the light passing through the reaction solution is mixed with the optical fiber positioned at one end so as to match the angle of the light reflected from one side of the reaction container. . As the light reflected from the reflector passes through the reaction solution once more, the light path through the reaction solution becomes longer, and thus the accurate absorbance can be measured. The light passing through the optical fiber can be detected by an image sensor positioned at the other end of the optical fiber and can display an image of light imaged on a display unit displaying an image connected to the image sensor. In one embodiment of the present invention, the image sensor is a charge-coupled device (CCD) or a complementary metal-oxide semiconductor (CMOS), although it is not limited thereto. The apparatus of the present invention can measure the real-time absorbance by storing the image at a predetermined time in the storage unit.
While the present invention has been described in connection with what is presently considered to be the preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, .
All technical terms used in the present invention are used in the sense that they are generally understood by those of ordinary skill in the relevant field of the present invention unless otherwise defined. The contents of all publications referred to herein are incorporated herein by reference.
10. Light Emitting Diodes
20. Tubular reaction vessel
22. Rectangular reaction vessel
24. Tubular reaction vessel with flat bottom surface
26. Reflector
30. Thermal block
40. Fiber
50. Image Sensor
60. Image display
100. Incident light
101. Reflected light
200. Reaction solution
Claims (9)
The apparatus comprises: a plurality of tubular reaction vessels;
A heating block having a plurality of tubular reaction vessels spaced apart from each other and having a plurality of channels penetrating at both longitudinal ends so that heat can be transferred to the side of the tubular reaction vessel;
A plurality of light emitting diodes (LEDs) positioned above each of the plurality of tubular reaction vessels and irradiating light in the longitudinal direction of the respective tubular reaction vessels;
A plurality of optical fibers having one end located at the bottom of each of the plurality of tubular reaction vessels and the other end located at the top of the image sensor;
An image sensor which is irradiated from the plurality of light emitting diodes and simultaneously detects light passing through the plurality of tubular reaction vessels and the optical fiber;
A display connected to the image sensor and displaying an image from the image sensor; And
And a storage unit for storing the images at predetermined time intervals.
Absorbance multi - measurement device for real - time molecular diagnosis.
The apparatus comprises: a plurality of tubular reaction vessels having a flat bottom;
A reflector which is in contact with the inside or outside of the bottom of the reaction vessel toward the inside of the tubular reaction vessel;
A heating block having a plurality of tubular reaction vessels in contact with the reflector spaced apart from each other and having a plurality of channels penetrating both longitudinal ends so that heat can be transferred to the longitudinal side of the tubular reaction vessel, ;
A plurality of light emitting diodes (LEDs) positioned above each of the plurality of tubular reaction vessels and irradiating light toward the reflectors of the respective tubular reaction vessels at a predetermined angle;
A plurality of optical fibers having one end located at the top of each of the plurality of tubular reaction vessels and the other end located at the top of the image sensor;
An image sensor that is irradiated from the plurality of light emitting diodes, passes through the plurality of tubular reaction vessels, is reflected by a reflector on the bottom of the reaction vessel, and simultaneously detects light passing through the optical fiber;
A display connected to the image sensor and displaying an image from the image sensor; And
And a storage unit for storing the images at predetermined time intervals.
Absorbance multi - measurement device for real - time molecular diagnosis.
The apparatus includes a plurality of rectangular parallelepiped reaction vessels;
A heating block having a plurality of rectangular parallelepiped reaction vessels spaced apart from each other and having a plurality of channels penetrating both sides in a transverse direction so that heat can be transferred to both sides and a lower portion of the rectangular parallelepiped reaction vessel;
A plurality of light emitting diodes (LEDs) positioned at side surfaces of each of the plurality of rectangular parallelepiped reaction vessels and irradiating light to one side of the rectangular parallelepiped reaction vessel having the longest optical coupling distance;
A plurality of optical fibers having one end disposed on one side of the plurality of rectangular parallelepiped reaction containers facing the light emitting diode (LED) and disposed opposite to the light emitting diode, and the other end positioned on the image sensor;
An image sensor which is irradiated from the plurality of light emitting diodes and simultaneously detects light passing through the plurality of tubular reaction vessels and the optical fiber;
A display connected to the image sensor and displaying an image from the image sensor; And
And a storage unit for storing the images at predetermined time intervals.
Absorbance multi - measurement device for real - time molecular diagnosis.
The apparatus includes a plurality of rectangular parallelepiped reaction vessels;
A reflector which is in contact with the inside or outside of the one face of the reaction vessel toward the interior of the rectangular parallelepiped reaction vessel;
A heating block having a plurality of rectangular parallelepiped reaction vessels to which the reflector is in contact and spaced apart from each other and having a plurality of channels penetrating both sides in a lateral direction so that heat can be transferred to both sides and a lower portion of the rectangular parallelepiped reaction vessel, );
A plurality of light emitting diodes (LEDs) positioned on opposite sides of the reflector of each of the plurality of rectangular parallelepiped reaction vessels and irradiating light toward the reflector at a predetermined angle;
A plurality of optical fibers positioned at one end of each of the plurality of rectangular parallelepiped reaction vessels on the opposite side of the reflector and the other end of the optical fiber positioned above the image sensor;
An image sensor which is irradiated from the plurality of light emitting diodes, passes through the plurality of rectangular parallelepiped reaction vessels, is reflected by a reflector on the opposite side, and simultaneously detects light passing through the optical fiber;
A display connected to the image sensor and displaying an image from the image sensor; And
And a storage unit for storing the images at predetermined time intervals.
Absorbance multi - measurement device for real - time molecular diagnosis.
The tubular reaction vessel may have a cylindrical shape, a rectangular column shape, a triangular column shape,
Absorbance multi - measurement device for real - time molecular diagnosis.
Wherein the rectangular parallelepiped reaction container has a width: length: height ratio of 1: 5: 1 to 1: 10: 5,
Absorbance multi - measurement device for real - time molecular diagnosis.
Wherein the predetermined angle is an angle of incidence of light emitted from the plurality of light emitting diodes,
Absorbance multi - measurement device for real - time molecular diagnosis.
The image sensor may be a CCD or CMOS,
Absorbance multi - measurement device for real - time molecular diagnosis.
The reaction of the reaction vessel may be a PCR or LAMP reaction,
Absorbance multi - measurement device for real - time molecular diagnosis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020140105915A KR20160020766A (en) | 2014-08-14 | 2014-08-14 | Multi-measuring apparatus of absorbance for realtime molecular diagnosis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020140105915A KR20160020766A (en) | 2014-08-14 | 2014-08-14 | Multi-measuring apparatus of absorbance for realtime molecular diagnosis |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101866922B1 (en) * | 2016-06-28 | 2018-06-15 | 한국과학기술연구원 | Apparatus and method for measuring water pollution based on multi-wavelength light source |
| KR20190097606A (en) | 2018-02-12 | 2019-08-21 | 한국과학기술연구원 | Compact multi molecular diagnosis apparatus |
| KR20190097605A (en) | 2018-02-12 | 2019-08-21 | 한국과학기술연구원 | Compact multi molecular diagnosis system |
| KR102285089B1 (en) * | 2020-03-02 | 2021-08-04 | 주식회사 더웨이브톡 | Detecting microorganisms apparatus |
| KR102295256B1 (en) * | 2020-03-02 | 2021-08-31 | 주식회사 더웨이브톡 | Sample block for testing and detecting microorganisms apparatus using the same |
| WO2023128647A1 (en) * | 2021-12-30 | 2023-07-06 | 메디칸 주식회사 | Device for measuring density of bio-nanoparticle including extracellular matrix, and method for measuring number of bio-nanoparticles using same |
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| KR20040048754A (en) | 2002-12-04 | 2004-06-10 | 뮤앤바이오 주식회사 | Temperature controlled real time fluorescence detection apparatus |
| KR20130113072A (en) | 2012-04-05 | 2013-10-15 | 고려대학교 산학협력단 | Apparatus for measuring absorbance using cmos image sensor |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20040048754A (en) | 2002-12-04 | 2004-06-10 | 뮤앤바이오 주식회사 | Temperature controlled real time fluorescence detection apparatus |
| KR20130113072A (en) | 2012-04-05 | 2013-10-15 | 고려대학교 산학협력단 | Apparatus for measuring absorbance using cmos image sensor |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101866922B1 (en) * | 2016-06-28 | 2018-06-15 | 한국과학기술연구원 | Apparatus and method for measuring water pollution based on multi-wavelength light source |
| KR20190097606A (en) | 2018-02-12 | 2019-08-21 | 한국과학기술연구원 | Compact multi molecular diagnosis apparatus |
| KR20190097605A (en) | 2018-02-12 | 2019-08-21 | 한국과학기술연구원 | Compact multi molecular diagnosis system |
| KR102285089B1 (en) * | 2020-03-02 | 2021-08-04 | 주식회사 더웨이브톡 | Detecting microorganisms apparatus |
| KR102295256B1 (en) * | 2020-03-02 | 2021-08-31 | 주식회사 더웨이브톡 | Sample block for testing and detecting microorganisms apparatus using the same |
| US11703449B2 (en) | 2020-03-02 | 2023-07-18 | The Wave Talk, Inc. | Microorganism detection apparatus |
| US11796474B2 (en) | 2020-03-02 | 2023-10-24 | The Wave Talk, Inc. | Test sample receiving block and microbial detection apparatus using the same |
| WO2023128647A1 (en) * | 2021-12-30 | 2023-07-06 | 메디칸 주식회사 | Device for measuring density of bio-nanoparticle including extracellular matrix, and method for measuring number of bio-nanoparticles using same |
| KR20230102232A (en) * | 2021-12-30 | 2023-07-07 | 메디칸(주) | Device for measuring the density of biological fine particles containing extracellular matrix and method for measuring the number of biological particles using the same |
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