KR20160009933A

KR20160009933A - Composition for cosmetics

Info

Publication number: KR20160009933A
Application number: KR1020140090486A
Authority: KR
Inventors: 문관형
Original assignee: 협동조합비앤비
Priority date: 2014-07-17
Filing date: 2014-07-17
Publication date: 2016-01-27

Abstract

The present invention relates to a cosmetic composition containing bee venom, propolis, royal jelly and a honey component, and the cosmetic composition of the present invention is particularly preferable for cosmetic soap, water rest, and the like.
The cosmetic composition of the present invention combines whitening function, antioxidative function and antibacterial function.

Description

COMPOSITION FOR COSMETICS

The present invention relates to a cosmetic composition containing bee venom, propolis, royal jelly and a honey ingredient. The present invention also relates to a cosmetic comprising such a cosmetic composition, a cosmetic soap and a wastepaper.

Melanin is a dark brown pigment that acts to protect the skin from ultraviolet rays because it has a function to block ultraviolet rays above a certain amount. Thus, when the skin is exposed to ultraviolet light, a large amount of melanin is produced in the body to protect the skin. Skin color is determined by the amount of melanin and the amount of melanin increases as the amount of the skin becomes black. Therefore, a large amount of melanin is caused by spots, dots, freckles, and black spots. Therefore, substances that inhibit melanin production may have a skin whitening effect.

On the other hand, when human skin becomes aging, aging phenomena appear in various parts. Common skin conditions can be represented by dull skin, wrinkles, dry skin, and the like. In order to solve this problem, it is necessary to solve various problems occurring on the skin surface, but more importantly, it is necessary to understand various aging mechanisms appearing in the skin, that is, dermis, epidermis, dermis and epidermis . A typical phenomenon of this aging mechanism is wrinkle formation and various efforts are being made to improve it. Representative methods include promoting the synthesis of collagen constituting the dermal layer or inhibiting the production or activity of MMP-1 (Matrix Metallo Proteinase-1) which promotes collagen degradation. In the cosmetics industry, natural materials or synthetic materials with these characteristics are used to apply to products and to improve wrinkles. Although these aging phenomena occur naturally over time, they can not be prevented 100%, but various methods can slow or prevent the occurrence of aging, and most cosmetics focus on preventing such aging.

Korean Patent Publication No. 10-2013-0089045 Korean Patent Registration 10-1225114

An object of the present invention is to provide a cosmetic composition having whitening and anti-aging properties and showing antibacterial activity.

Another object of the present invention is to provide a cosmetic, a cosmetic soap and a wastepaper comprising such a functional cosmetic composition.

The object of the present invention is achieved by a cosmetic composition containing bee venom, propolis, royal jelly and a honey ingredient.

In addition, the present invention includes cosmetics containing bee venom, propolis, royal jelly and honey components, cosmetic soap, and water toilet.

The cosmetic composition of the present invention combines whitening function, antioxidative function and antibacterial function.

The present invention relates to a cosmetic composition containing bee venom, propolis, royal jelly and a honey ingredient. Since the cosmetic composition of the present invention is excellent in antibacterial effect, it is particularly preferable to use it in the form of soap or water.

In the composition, bee venom or propolis is excellent in antioxidative and antimicrobial effects and can be used in an amount of 10 to 40% by weight, respectively. Royal jelly and honey are excellent in whitening and antioxidant effect and can be used in an amount of 10 to 40% by weight, respectively.

Hereinafter, the present invention will be described in more detail with reference to the following examples and test examples.

Example 1

250 g of bee venom, 250 g of propolis, 250 g of royal jelly and 250 g of honey were mixed to obtain 1 kg of a mixed composition.

Example 2

400 g of bee venom, 400 g of propolis, 100 g of royal jelly and 100 g of honey were mixed to obtain 1 kg of a mixed composition.

Example 3

100 g of bee venom, 100 g of propolis, 400 g of royal jelly and 400 g of honey were mixed to obtain 1 kg of a mixed composition.

Example 4

100 g of bee venom, 400 g of propolis, 100 g of royal jelly and 400 g of honey were mixed to obtain 1 kg of a mixed composition.

<Experimental Example 1> Measurement of tyrosinase inhibitory activity

Tyrosinase activity was used with minor modifications to the method of Pomerants S. H., 1963 J. Biol. Chem., 238, 2351-2357. 40 μl of a 5 mM L-DOPA solution, 80 μl of 67 mM phosphate buffer (pH 6.8), and 40 μl of a test sample solution (Examples 1 to 4 and a test solution concentration of 5 μg / ml of arbutin) were mixed. Then, 40 [mu] l (100 units / ml)

Of human tyrosinase was added to prepare a sample sample.

The final concentration of the test sample solution thus obtained was 1 mg / ml. To make the final concentration of the test solution to be 0.5 mg / ml, 40 μl of 5 mM L-DOPA solution, 100 μl of 67 mM phosphate buffer (pH 6.8) and 20 μl (Examples 1 to 4, and test of arbutin Solution concentration of 2.5 mg / ml) were mixed, and then 40 μl (100 units / ml) of mushroom tyrosinase was added to the reaction mixture to prepare sample samples.

In the preparation of the sample, the test sample solution was not added and used as a control. When the enzyme activity of the control group was taken as 100, the inhibitory activity of each enzyme was expressed as% in comparison with the control group. In order to observe the relative intensity of the tyrosinase inhibitory activity of the compositions of Examples 1 to 4, the relative activities with respect to arbutin, which is widely used for whitening cosmetics, were compared.

When the contents were 0.5 mg / ml and 1 mg / ml, arbutin inhibitory activity was 18.5% and 54.5%, respectively.

Example 1 was 18.2% and 56.1%, similar to arbutin, and Example 3 was 20.3% and 59.0% superior to arbutin. Examples 2 and 4 showed tyrosinase inhibitory activity, albeit lower than that of arbutin.

<Experimental Example 2> Melanin synthesis inhibitory effect

B16 melanoma cell culture

B16 melanoma cells were purchased from KTCC (The Korean collection for Type Culture). B16 Melanoma cells are melanin-producing cell lines and are generally used for screening whitening substances.

DMEM medium containing 10% FBS, 1% penicillin / streptomycin was prepared and sterilized to make the medium for culturing the cells aseptic. Cell cultures were incubated in an incubator at 37 ° C, 5% CO 2. Melanoma cells were cultured for about 3 days and passaged when 100 ㎎ Patrydish was about 90% full.

Measurement of inhibition of melanin synthesis by absorbance measurement

For the whitening effect measurement of the cosmetic composition of the present invention, B16 melanoma cells

Was treated with the same amount of each of the compositions of Examples 1 to 4 or arbutin, and the change in the amount of melanin produced was measured. Cells were inoculated in a 96-well plate and after 24 hours, the compositions of Examples 1 to 4 or arbutin were treated at a constant concentration and cultured for another 48 hours. Then, cells were recovered using trypsin-EDTA. The recovered cells were reacted with 1 N NaOH containing 10% DMSO (dimethyl sulfoxide) at 80 ° C for 1 hour to allow the melanin to fully dissolve. Then, the cells were subjected to enzyme-linked immunosorbent assay (ELISA) Absorbance was measured at 405 nm to calculate melanin production. As a result, in the case of arbutin known as a general whitening substance, the amount of melanin was 72.2% at a concentration of 500 / / ml, compared with 68.4% of melanin in Example 3 of the present invention, 70.9%, respectively.

&Lt; Experimental Example 3 >

Free radical scavenging activity can be measured in order to confirm the antioxidative activity of the herbal fermented extract of each of Examples 1 to 4 above.

The test samples are the fermented herbal extracts of Examples 1 to 18 and the free radical scavenging activity is measured using DPPH. DPPH is purchased from Sigma Co., Ltd. (USA). First, a standard DPPH solution having a concentration of 400 mu m is prepared. Then, ethanol was added to ascorbic acid, which is an antioxidant, as a reference material and the composition of Examples 1 to 4, respectively, to prepare a sample at a concentration of 200 μg / ml.

Next, the sample and standard DPPH solution were added at the same ratio, stirred well, reacted at 37 ° C for 30 minutes, and the absorbance at 520 nm was measured to determine the free radical scavenging ratio (%).

The antioxidative rate was about 67% to 70%, which did not show a large difference in Examples 1 to 4.

The compositions of each example gave better antimicrobial effects to staphylococci over other human pathogens. Example 3 was carried out using Streptococcus (hereinafter referred to as "

S. pyogens) A77 showed the antimicrobial effect of the lowest inhibitory concentration of 2.0 mg / ml, while Example 2 showed an excellent antibacterial effect of 0.006 mg / ml. Includes furuncle, carbuncle, cellulitis,

It is a bacterium that mainly causes purulent inflammation such as solitary tonsillitis, coryza infection, pharyngitis, laryngitis, otitis media and osteomyelitis. Therefore, the cosmetic composition containing the composition of the present invention has a great effect for inhibiting pathogenic bacteria of human skin causing skin diseases. Therefore, it is very useful when it is used as cosmetic soap, water tissue, or the like.

Claims

Bee venom, propolis, royal jelly and honey.

Wherein the bee venom, the propolis, the royal jelly and the honey are each contained in an amount of 10 to 40% by weight of the bee venom.

A cosmetic soap comprising the cosmetic composition of claim 1.

A water tissue comprising the cosmetic composition of claim 1.