KR20160004461A - Bio sensor for including sample inlet located upper plate - Google Patents

Bio sensor for including sample inlet located upper plate Download PDF

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Publication number
KR20160004461A
KR20160004461A KR1020140082512A KR20140082512A KR20160004461A KR 20160004461 A KR20160004461 A KR 20160004461A KR 1020140082512 A KR1020140082512 A KR 1020140082512A KR 20140082512 A KR20140082512 A KR 20140082512A KR 20160004461 A KR20160004461 A KR 20160004461A
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KR
South Korea
Prior art keywords
sample
biosensor
sample inlet
plate
accumulated
Prior art date
Application number
KR1020140082512A
Other languages
Korean (ko)
Inventor
이승노
권민수
경종민
Original Assignee
주식회사 엠아이텍
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Application filed by 주식회사 엠아이텍 filed Critical 주식회사 엠아이텍
Priority to KR1020140082512A priority Critical patent/KR20160004461A/en
Publication of KR20160004461A publication Critical patent/KR20160004461A/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"

Abstract

A biosensor for measuring an electrochemical reaction between a sample and a biosensing component includes: a top plate having at least one sample inlet through which a sample flows and at least one air outlet through which air is discharged; A middle plate including a space in which the sample introduced through the at least one sample inlet is accumulated; And a lower plate on which a biosensing component is disposed to generate an electrochemical reaction between the sample and the biosensing component.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a biosensor including a sample inlet located in an upper plate,

The present invention relates to a biosensor including a sample inlet located on a top plate, and more particularly, to a biosensor including a biosensor and a top plate on which a sample inlet for introducing a sample for generating an electrochemical reaction is vertically arranged, .

Biosensors for detecting electrochemical reactions between a sample and a biosensing component are widely used in the medical field to analyze biological samples including blood. In particular, a biosensor using an enzyme as a biosensing component is most widely used because it is easy to apply, has excellent measurement sensitivity, and can obtain quick results. Here, an electrode method for analyzing an enzyme applied to a biosensor is an electrode method in which an electrode system is formed, an enzyme is fixed on an electrode, and a current generated by applying a constant voltage after the sample is introduced is used.

However, in the conventional biosensor, since the sample inlet through which the sample is introduced is disposed horizontally with the biosensor, there is an inconvenience in the user entering the sample. In addition, when the user tilts the biosensor vertically in order to conveniently flow the sample, the biosensing component disposed in the biosensor tends to deviate, so that accurate measurement can not be performed.

Thus, in this specification, a biosensor including a top plate in which a sample inlet is arranged in a vertical direction is proposed in order to improve the convenience of sample introduction.

An embodiment of the present invention provides a biosensor in which the convenience of inflow of a sample is improved by disposing a sample inlet in a vertical direction on a top plate.

According to another aspect of the present invention, there is provided a biosensor having improved efficiency of discharging air by disposing an air discharge port through which air is discharged in a direction perpendicular to an upper plate or horizontally disposed on a side surface of a middle plate.

In addition, one embodiment of the present invention provides a biosensor in which the rate of flow of a sample is improved by applying a hydrophilic coating.

The biosensor for measuring an electrochemical reaction between a sample and a biosensing component according to an embodiment of the present invention includes at least one sample inlet through which a sample is introduced and at least one air outlet through which air is discharged, ; A middle plate including a space in which the sample introduced through the at least one sample inlet is accumulated; And a lower plate on which a biosensing component is disposed and in which a chemical reaction occurs between the sample and the biosensing component.

Hydrophilic coating may be applied to the portion of the upper plate which contacts the space where the sample is accumulated.

The lower plate may include a pair of sensing electrodes for sensing the inflow of the sample, a working electrode to which power is applied to measure the electrochemical reaction, and a reference electrode.

A biosensor for measuring an electrochemical reaction between a sample and a biosensing component according to an embodiment of the present invention includes: a top plate in which at least one sample inlet through which a sample flows is vertically arranged; A middle plate including a space in which the sample introduced through the at least one sample inlet is accumulated and at least one air outlet through which air is discharged; And a lower plate on which a biosensing component is disposed and in which an electrochemical reaction occurs between the sample and the biosensing component, and the at least one air outlet is horizontally disposed on a side surface of the middle plate.

Hydrophilic coating may be applied to the portion of the upper plate which contacts the space where the sample is accumulated.

The lower plate may include a pair of sensing electrodes for sensing the inflow of the sample, a working electrode to which power is applied to measure the electrochemical reaction, and a reference electrode.

According to one embodiment of the present invention, a sample inlet can be vertically arranged on a top plate, thereby providing a biosensor with improved sample inlet efficiency.

According to another aspect of the present invention, there is provided a biosensor having improved efficiency of discharging air by disposing an air discharging port through which air is discharged in a vertical direction on a top plate or horizontally on a side surface of a middle plate.

In addition, one embodiment of the present invention can provide a biosensor in which a sample is introduced at a high speed by applying a hydrophilic coating.

1 is a view showing a biosensor in which an air outlet according to an embodiment of the present invention is disposed in a direction perpendicular to a top plate.
FIG. 2 is a side view showing the biosensor shown in FIG. 1. FIG.
3 is a view illustrating a biosensor in which an air outlet according to an embodiment of the present invention is disposed horizontally on an intermediate plate.
FIG. 4 is a top view and a cross-sectional view of a middle plate included in the biosensor shown in FIG. 3;
5 is a side view showing the biosensor shown in FIG.

Hereinafter, embodiments according to the present invention will be described in detail with reference to the accompanying drawings. However, the present invention is not limited to or limited by the embodiments. In addition, the same reference numerals shown in the drawings denote the same members.

1 is a view showing a biosensor in which an air outlet according to an embodiment of the present invention is disposed in a direction perpendicular to a top plate.

Referring to FIG. 1, a biosensor according to an embodiment of the present invention includes a top plate 110, a middle plate 120, and a bottom plate 130. Here, at least one sample inlet 111 through which the sample flows into the upper plate 110 and at least one air outlet 112 through which the air is discharged in response to the introduction of the sample are vertically arranged. At least one of the sample inlet 111 and the at least one air outlet 112 may be formed by vertically inserting the panel of the upper plate 110 or inserting the tube in the vertical direction.

The middle plate 120 includes a space 121 through which at least one sample introduced through the sample inlet 111 is accumulated. At this time, the space 121 in which the sample is accumulated may be connected to each of the at least one sample inlet 111 and the at least one air outlet 112.

Here, a hydrophilic coating may be applied to a portion of the upper plate 110 which contacts the space 121 in which the sample included in the middle plate 120 is accumulated. This will be described in detail with reference to Fig.

An electrochemical reaction may occur between the sample and the biosensing component by disposing a biosensing component in the portion 131 of the lower plate 130 that contacts the space 121 where the sample is stored. In this case, the lower plate 130 may include a pair of sensing electrodes 132 for sensing the flow of the sample, a working electrode 133 to which power is supplied for measuring the electrochemical reaction, and a reference electrode 134 .

As described above, the at least one sample inlet 111 through which the sample included in the biosensor according to an embodiment of the present invention is arranged in a direction perpendicular to the top plate 110 can improve the convenience of sample introduction . Further, since the biosensing component disposed on the lower plate 130 is prevented from leaning, the reliability of measurement can be increased. In addition, contamination of the biosensing component can be prevented during the process of fabricating at least one sample inlet 111 and the sample inlet through at least one sample inlet 111.

FIG. 2 is a side view showing the biosensor shown in FIG. 1. FIG.

2, the biosensor according to an embodiment of the present invention includes an upper plate 210, a middle plate 220, and a lower plate 230. [ At this time, the upper plate 210, the middle plate 220, and the lower plate 230 may be attached to each other by an adhesive 240.

At least one sample inlet 211 into which the sample flows and at least one air outlet 212 through which the air is discharged are vertically arranged in the upper plate 210. Therefore, the convenience of the process of introducing the sample through the at least one sample inlet 211 can be improved.

The middle plate 220 includes a space 221 through which at least one sample introduced through the sample inlet 211 is accumulated. At this time, the space 221 where the sample is accumulated may be connected to each of the at least one sample inlet 211 and the at least one air outlet 212.

A hydrophilic coating 213 is applied to a portion of the upper plate 210 contacting the space 221 in which the sample contained in the middle plate 220 is accumulated so that the sample is accumulated through the at least one sample inlet 211, It is possible to improve the flow rate of the air into the space 221. Therefore, since the electrochemical reaction between the sample and the biosensing component 231 also occurs earlier than the conventional biosensor, the process of measuring the electrochemical reaction can be performed earlier than the conventional biosensor.

The lower plate 230 is provided with a biosensing component 231 at a portion contacting the space 221 in which the sample is stored and includes a pair of recognition electrodes 232 for sensing the inflow of the sample, A working electrode 233 and a reference electrode 234 to which power is supplied can be disposed.

3 is a view illustrating a biosensor in which an air outlet according to an embodiment of the present invention is disposed horizontally on an intermediate plate.

Referring to FIG. 3, the biosensor according to an embodiment of the present invention includes an upper plate 310, a middle plate 320, and a lower plate 330. Here, at least one sample inlet 311 through which the sample flows into the upper plate 310 is arranged in the vertical direction. At least one sample inlet 311 is formed by drilling a panel of the upper plate 310 in a vertical direction and inserting a tube in a vertical direction so that a space 321 in which the sample contained in the middle plate 320 is accumulated, Can be connected.

The middle plate 320 is connected to a space 321 through which the sample flowing through the at least one sample inlet 311 is accumulated and a space 321 through which the sample is accumulated so that at least one And an air outlet 322 of the air outlet 322. At this time, the at least one air outlet 322 is horizontally disposed on the side surface of the middle plate 320. At least one air outlet 322 may be created by drilling the panel of the middle plate 320 in the horizontal direction or by inserting the pipe in the horizontal direction.

Here, a hydrophilic coating may be applied to a portion of the upper plate 310 that abuts the space 321 in which the sample contained in the middle plate 320 is accumulated. This will be described in detail with reference to FIG.

In addition, an electrochemical reaction may occur between the sample and the biosensing component by disposing the biosensing component in the portion 331 contacting the space 321 in which the sample is accumulated. At this time, the lower plate 330 may include a pair of recognition electrodes 332 for sensing the flow of the sample, a working electrode 333 to which power is applied to measure an electrochemical reaction, and a reference electrode 334 .

As described above, the at least one sample inlet 311 through which the sample contained in the biosensor according to the embodiment of the present invention is introduced is disposed perpendicularly to the top plate 310, . In addition, since the biosensing component disposed on the lower plate 330 is prevented from leaning, the reliability of measurement can be increased. In addition, contamination of the biosensing component can be prevented during the process of fabricating at least one sample inlet 311 and the sample inlet through at least one sample inlet 311.

Also, since at least one air outlet 332 is arranged from the at least one sample inlet 311 to the other side, in the course of the user entering the sample into the at least one sample inlet 311, The inflow of the sample can be prevented.

FIG. 4 is a top view and a cross-sectional view of a middle plate included in the biosensor shown in FIG. 3;

4, the middle plate included in the biosensor according to an embodiment of the present invention is connected to a space 410 through which a sample introduced through at least one sample inlet port is accumulated and a space 410 through which a sample is accumulated And at least one air outlet 420 through which air is discharged in response to the sample being introduced.

At least one air outlet 420 included in the middle plate may be formed so as to connect with the space 410 where the sample is accumulated by drilling the panel constituting the middle plate in the horizontal direction or by inserting the tube in the horizontal direction.

In addition, at least one air outlet 420 included in the middle plate is formed not only on the panel constituting the middle plate, but also a part of the adhesive 430 existing between the middle plate and the upper plate in the horizontal direction Or by inserting a pipe in the horizontal direction, so as to be connected to the space 410 where the sample is accumulated.

5 is a side view showing the biosensor shown in FIG.

5, a biosensor according to an embodiment of the present invention includes a top plate 510, a middle plate 520, and a bottom plate 530. [ At this time, the upper plate 510, the middle plate 520, and the lower plate 530 may be attached to each other by an adhesive 540.

At least one sample inlet 511 through which the sample flows is arranged in the vertical direction in the upper plate 510. Therefore, the convenience of the process of introducing the sample through the at least one sample inlet 511 can be improved.

The middle plate 520 is connected to a space 521 in which the sample introduced through the at least one sample inlet 511 is accumulated and a space 521 in which the sample is stored so that at least one And an air outlet 522 of the air outlet 522. At this time, at least one air outlet 522 is horizontally disposed on the side surface of the middle plate 520. Thus, since at least one air outlet 522 is arranged from the at least one sample inlet 511 to the other side, in the course of the user entering the sample into the at least one sample inlet 511, The sample can be prevented from flowing into the chamber 522.

The hydrophilic coating 512 is applied to a portion of the upper plate 510 that contacts the space 521 in which the sample contained in the middle plate 520 is accumulated so that the sample is accumulated through the at least one sample inlet 511, It is possible to improve the flow rate into the space 521. Therefore, since the electrochemical reaction between the sample and the biosensing component 531 occurs earlier than the conventional biosensor, the process of measuring the electrochemical reaction can also be performed earlier than the conventional biosensor.

The lower plate 530 is provided with a pair of sensing electrodes 532 for sensing the inflow of the sample, a biosensing component 531 disposed at a portion contacting the space 521 in which the sample is accumulated, A working electrode 533 and a reference electrode 534 to which power is supplied may be disposed.

The apparatus described above may be implemented as a hardware component, a software component, and / or a combination of hardware components and software components. For example, the apparatus and components described in the embodiments may be implemented within a computer system, such as, for example, a processor, a controller, an arithmetic logic unit (ALU), a digital signal processor, a microcomputer, a field programmable array (FPA) A programmable logic unit (PLU), a microprocessor, or any other device capable of executing and responding to instructions. The processing device may execute an operating system (OS) and one or more software applications running on the operating system. The processing device may also access, store, manipulate, process, and generate data in response to execution of the software. For ease of understanding, the processing apparatus may be described as being used singly, but those skilled in the art will recognize that the processing apparatus may have a plurality of processing elements and / As shown in FIG. For example, the processing unit may comprise a plurality of processors or one processor and one controller. Other processing configurations are also possible, such as a parallel processor.

The software may include a computer program, code, instructions, or a combination of one or more of the foregoing, and may be configured to configure the processing device to operate as desired or to process it collectively or collectively Device can be commanded. The software and / or data may be in the form of any type of machine, component, physical device, virtual equipment, computer storage media, or device , Or may be permanently or temporarily embodied in a transmitted signal wave. The software may be distributed over a networked computer system and stored or executed in a distributed manner. The software and data may be stored on one or more computer readable recording media.

The method according to an embodiment may be implemented in the form of a program command that can be executed through various computer means and recorded in a computer-readable medium. The computer-readable medium may include program instructions, data files, data structures, and the like, alone or in combination. The program instructions to be recorded on the medium may be those specially designed and configured for the embodiments or may be available to those skilled in the art of computer software. Examples of computer-readable media include magnetic media such as hard disks, floppy disks and magnetic tape; optical media such as CD-ROMs and DVDs; magnetic media such as floppy disks; Magneto-optical media, and hardware devices specifically configured to store and execute program instructions such as ROM, RAM, flash memory, and the like. Examples of program instructions include machine language code such as those produced by a compiler, as well as high-level language code that can be executed by a computer using an interpreter or the like. The hardware devices described above may be configured to operate as one or more software modules to perform the operations of the embodiments, and vice versa.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. For example, it is to be understood that the techniques described may be performed in a different order than the described methods, and / or that components of the described systems, structures, devices, circuits, Lt; / RTI > or equivalents, even if it is replaced or replaced.

Therefore, other implementations, other embodiments, and equivalents to the claims are also within the scope of the following claims.

Claims (6)

A biosensor for measuring an electrochemical reaction between a sample and a biosensing component,
At least one sample inlet through which a sample flows and at least one air outlet through which air is discharged are arranged in a vertical direction;
A middle plate including a space in which the sample introduced through the at least one sample inlet is accumulated; And
A biosensing component is disposed on the lower surface of the substrate,
. The method according to claim 1,
A portion of the upper plate, which abuts the space in which the sample is accumulated,
A biosensor to which a hydrophilic coating is applied. The method according to claim 1,
The lower plate
A pair of sensing electrodes for sensing the flow of the sample, a working electrode to which power is applied to measure the electrochemical reaction, and a reference electrode. A biosensor for measuring an electrochemical reaction between a sample and a biosensing component,
A top plate in which at least one sample inlet through which the sample flows is arranged in a vertical direction;
A middle plate including a space in which the sample introduced through the at least one sample inlet is accumulated and at least one air outlet through which air is discharged; And
A biosensing component is disposed on the lower surface of the substrate,
/ RTI >
The at least one air outlet
Wherein the biosensor is disposed horizontally on a side surface of the middle plate. 5. The method of claim 4,
A portion of the upper plate, which abuts the space in which the sample is accumulated,
A biosensor to which a hydrophilic coating is applied. 5. The method of claim 4,
The lower plate
A pair of sensing electrodes for sensing the flow of the sample, a working electrode to which power is applied to measure the electrochemical reaction, and a reference electrode.
KR1020140082512A 2014-07-02 2014-07-02 Bio sensor for including sample inlet located upper plate KR20160004461A (en)

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Application Number Priority Date Filing Date Title
KR1020140082512A KR20160004461A (en) 2014-07-02 2014-07-02 Bio sensor for including sample inlet located upper plate

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210075746A (en) * 2019-12-13 2021-06-23 (주)셀라바이오텍 Vertical particle quantitive measurement apparatus based on potential measurement using nano ink and method therefore

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210075746A (en) * 2019-12-13 2021-06-23 (주)셀라바이오텍 Vertical particle quantitive measurement apparatus based on potential measurement using nano ink and method therefore

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