KR20150126215A - A Pharmaceutical Agent For Treatment Of Alopecia Containing Yeast Constutuent Extract And The Manufacturing Method Thereof - Google Patents

Abstract

The present invention relates to a therapeutic agent for alopecia using a yeast extract, and a method for manufacturing the same and, more specifically, to a therapeutic agent for alopecia manufactured by using beer yeasts or raw rice wine (makgeolli) yeasts, and a method for manufacturing the same. The therapeutic agent for alopecia using the yeast extract of the present invention contains more than one yeast component among the beer yeasts and the makgeolli yeasts. In addition, the method for manufacturing the therapeutic agent for alopecia using the yeast extract comprises: a first step of fermenting yeasts in a medium; a second step of centrifuging the yeasts and the medium, and removing a supernatant, and then separating the yeast component from sediment; a third step of removing aqueous smell components by washing the yeast component from the second step with distilled water; a fourth step of centrifuging the yeast component from the third step, and removing the supernatant; a fifth step of removing alcohol-soluble smell components by washing the yeast component from the fourth step with alcohol; a sixth step of centrifuging the yeast component from the fifth step, and removing the supernatant; a seventh step of swelling yeast cells by dispersing the yeast component from the sixth step in distilled water; an eighth step of lysing the yeast cells from the seventh step so as to diffuse the yeast cells and a lysate thereof in the distilled water; a ninth step of centrifuging the distilled water from the eighth step, and removing non-aqueous sediment, and then collecting an aqueous supernatant; a tenth step of sterilizing the aqueous supernatant so as to remove microorganisms capable of causing decomposition; and an eleventh step of manufacturing the yeast extract by freezing or lyophilizing the aqueous supernatant from the tenth step.

Description

[0001] The present invention relates to a pharmaceutical agent for alopecia and a method for producing the same,

The present invention relates to a hair loss therapeutic agent using a yeast component extract and a method for preparing the same, and more particularly, to a hair loss treatment agent prepared using a brewer's yeast or a makgeolli yeast ingredient and a method for producing the same.

As the quality of life has improved recently, interest in cosmetics has been excessively increased. Accordingly, effective skin-care medicines such as cosmetics, soaps, nutritional supplements, and hair loss remedies have also been used in cosmetics or cosmetics, It is true.

However, there have been cases in which skin damage or side effects are caused during use of the cosmetic agent or therapeutic agent as described above. In this case, the use of a cosmetic agent containing a chemical agent having a quicker effect than a natural agent- The frequency can be high.

In the conventional art, when a hair loss therapeutic agent or a skin treatment agent is produced, a chemical ingredient is generally added to enhance the effect. When such a chemical ingredient is used excessively, there is a problem that the scalp becomes more dry or stimulates the scalp.

Korean Patent No. 10-0822028

The object of the present invention is to solve the above-mentioned problems, and an object of the present invention is to provide a yeast extract having no odor and sediment by using brewer's yeast or rice wine yeast, Thereby providing a therapeutic agent.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not restrictive of the invention as set forth in the accompanying drawings. It will be possible.

In order to achieve the above-mentioned object, the present invention is characterized in that the hair loss treatment agent using the yeast component extract of the present invention comprises at least one yeast component from a rice wine yeast and a brewer's yeast.

In addition, the method of manufacturing a therapeutic agent for alopecia using the yeast extract of the present invention comprises the steps of: 1) fermenting yeast in a culture solution; A second step of separating the yeast component and the precipitate after the supernatant is removed by centrifuging the yeast and the culture solution; A third step of washing the yeast component of the second step with distilled water to remove the water-soluble odor component; A fourth step of removing the supernatant by centrifuging the yeast component of step 3; A fifth step of washing the yeast component of step 4 with alcohol to remove the alcohol soluble odor component; A sixth step of centrifuging the yeast component of step 5 to remove the supernatant; A seventh step of expanding the cells by dispersing the yeast component of step 6 in distilled water; An eighth step of disrupting cells of the yeast component in step 7 to diffuse the yeast cell component and the crushed product in distilled water; Separating the water-soluble supernatant after removing the non-aqueous precipitate by centrifuging the distilled water in the eighth step; A step 10 of sterilizing the aqueous supernatant to remove microorganisms that may be decayed; And an eleventh step of preparing the yeast component extract by freezing or freeze-drying the aqueous supernatant of step 10).

The hair loss treatment agent using the yeast component extract of the present invention has the following effects.

By applying the yeast component extract prepared by using the rice wine yeast or brewer's yeast to the scalp, the nutrients rich in the yeast ingredient promote the hair growth and prevent hair loss.

In addition, in the step of extracting the yeast component extract, the odor component and the precipitate are removed to increase the preference of the consumers, and the microorganisms in the extract are removed, thereby providing a therapeutic agent for depilation of hair that is not spoiled.

1 is a flow chart showing a method for preparing a hair loss therapeutic agent using a yeast component extract of the present invention.
Fig. 2 is a photograph taken before application of the hair loss remedy of the present invention to mouse 1. Fig.
FIG. 3 is a photograph of the hair loss remedy of the present invention after being applied to mouse 1 for 6 weeks.
Fig. 4 is a photograph taken before application of the hair loss remedy of the present invention to mouse 2. Fig.
FIG. 5 is a photograph of the hair loss remedy of the present invention after being applied to mouse 2 for 6 weeks.
Fig. 6 is a photograph taken before application of the hair loss treatment agent of the present invention to Experimental Circle 1. Fig.
FIG. 7 is a photograph of the hair loss remedy of the present invention after being applied to Experiment circle 1 for 6 months.
FIG. 8 is a photograph taken before application of the hair loss treatment agent of the present invention to Experiment circle 2. FIG.
FIG. 9 is a photograph of the hair loss remedy of the present invention taken after the applicant 2 was applied for 6 months.

The anti-depressant agent using the yeast component extract according to the present invention is characterized in that it comprises at least one yeast component from the rice wine yeast and the brewer's yeast.

Hereinafter, a method for producing a hair loss therapeutic agent using the yeast component extract will be described with reference to drawings and Examples.

≪ Preparation method of hair loss therapeutic agent using yeast component extract >

FIG. 1 is a flow chart showing a method of manufacturing a hair loss therapeutic agent using the yeast component extract of the present invention.

First, in a first step (S101), the yeast is fermented in a culture solution. Specifically, the yeast extracted from at least one of beer and makgeolli is fermented in a culture solution.

Next, in the second step (S102), the yeast and the culture are centrifuged to remove the supernatant, and then the yeast component and the precipitate are separated. Specifically, the fermented yeast is centrifuged to remove the supernatant, and the yeast component and the precipitate are separated from each other to obtain a yeast component.

Next, in the third step (S103), the yeast component of the second step is washed with distilled water to remove the water-soluble odor component. Specifically, the yeast component obtained in step 2 is washed with distilled water one to three times. If the cleaning operation using distilled water is not performed, the water soluble odor component can not be sufficiently dissolved in the yeast component. If the washing operation is performed more than 3 times, the efficiency of the operation may be deteriorated.

Next, in the fourth step (S104), the yeast components in the above three stages are centrifuged to remove the supernatant. Specifically, the yeast component from the three steps is centrifuged to separate and remove the water-soluble odor component.

Next, in the fifth step (S105), the yeast component in the step 4 is washed with alcohol to remove the alcohol soluble odor component. Specifically, the alcohol-soluble odor component contained in the yeast component of step 4 is washed with alcohol.

In addition, the alcohol penetrates the membrane of the bacterial surface with the osmotic power, enters the germs, and serves to coagulate the protein, which is the life basis of the germ, to remove the bacteria.

Generally used alcohol is 70% alcohol with ethanol. If the concentration of alcohol exceeds 70%, the ability of the bacteria to coagulate the protein is very good. However, since the proteins on the surface of the bacteria are instantaneously coagulated to form a hard film on the outer wall of the bacteria, the alcohol is prevented from penetrating into the inside of the bacteria do. When the concentration of alcohol is less than 70%, it is difficult to completely remove bacteria because the ability of the bacteria to coagulate the protein is lowered. Therefore, it is preferable to use alcohol at a concentration of 70%.

Next, in the sixth step (S106), the yeast component in the fifth step is centrifuged to remove the supernatant. Specifically, the yeast component washed with the alcohol is centrifuged to separate and remove the alcohol-soluble odor component.

Next, in a seventh step (S107), the yeast component of the sixth step is dispersed in distilled water to expand the cells. Specifically, the yeast component is dispersed in distilled water, and the cells are expanded by osmotic pressure.

Next, in the eighth step (S108), the cells of the yeast component in the seventh step are disrupted to diffuse the yeast cell component and the crushed product in the distilled water. Specifically, the expanded cells are disrupted by at least one of an ultrasonic wave, a microfluidizer, a glass bead, and a French press so that the yeast cell component and the crushed product are diffused in the distilled water.

Only the effective constituents in yeast cells can be selectively extracted during the crushing process. That is, it is possible to selectively extract only effective components in yeast cells by disrupting cells that have undergone the expansion process after previously removing precipitates and suspensions in the above process.

Next, in the ninth step (S109), the distilled water in the eighth step is centrifuged to remove the non-aqueous precipitate, and the aqueous supernatant is collected. Specifically, the distilled water in which the yeast cell component and the crushed product are diffused is centrifuged, and then the non-aqueous precipitate precipitated below is removed to collect the aqueous supernatant.

Next, in the tenth step (S110), the aqueous supernatant is sterilized. Specifically, sterilization is carried out using an autoclave in order to remove microorganisms which may be spoilable in the aqueous supernatant.

Next, in the eleventh step (S111), the aqueous soluble supernatant of the tenth step is frozen or lyophilized to prepare a yeast component extract. Specifically, the sterilized aqueous supernatant is frozen and stored to prepare a liquid yeast component extract or lyophilized to prepare a powdery yeast component extract.

The yeast component extract can be directly applied to the scalp to serve as a hair loss treatment agent showing hair growth enhancement and hair loss prevention effect.

<Demonstration effect of hair loss treatment>

In the following, clinical experiments are performed and quality tests for hair growth promotion and hair loss prevention effects of the hair loss treatment agent using the yeast component extract of the present invention are described with reference to the drawings.

The experiment was conducted two times in each experimental group with the mouse and the hair loss disease. The photographs were taken before applying the hair loss treatment agent, and the hair loss treatment agent was applied for a certain period of time and the area of the hair was re- The results are shown below.

First, the result of an experiment on mouse 1 for the hair loss treatment agent of the present invention.

Fig. 2 is a photograph taken before applying the depilatory remedy of the present invention to mouse 1. Fig.

FIG. 3 is a photograph of the hair loss remedy of the present invention after being applied to mouse 1 for 6 weeks.

As a result of comparing FIG. 2 and FIG. 3, it can be seen visually that black hair increases in the abdomen portion of the mouse treated with hair treatment agent.

Next, the result of an experiment on the mouse 2 for the hair loss treatment agent of the present invention.

FIG. 4 is a photograph taken before application of the hair loss treatment agent of the present invention to mouse 2. FIG.

FIG. 5 is a photograph of the hair loss remedy of the present invention after being applied to mouse 2 for 6 weeks.

As a result of comparison between FIG. 4 and FIG. 5, it was visually recognized that the black hair increased in the abdomen portion of the rats coated with the hair treatment agent.

Next, the result of an experiment for the inventive hair loss treatment agent of Experimental Circle 1 is shown.

Fig. 6 is a photograph taken before application of the hair loss treatment agent of the present invention to Experimental Circle 1. Fig.

Fig. 7 is a photograph taken after applying the hair loss treatment agent of the present invention to Experiment circle 1 for 6 months.

As a result of comparing FIG. 6 and FIG. 7, it can be seen that the number and length of the hair in the scalp portion coated with the hair treatment agent were remarkably increased.

Next, the result of an experiment on the hair loss treatment agent of the present invention to Experiment circle 2.

FIG. 8 is a photograph taken before applying the hair loss treatment agent of the present invention to the experimental circle 2.

Fig. 9 is a photograph of the hair loss remedy of the present invention after being applied to Experiment circle 2 for 6 months.

As a result of comparing FIG. 8 and FIG. 9, it can be seen that the number and length of hair in the scalp portion coated with the hair treatment agent are remarkably increased.

2 to 9, it can be seen that the hair loss treatment agent of the present invention includes hair growth promoting and hair loss prevention effects as described above.

S101. Step 1: fermenting the yeast in the culture medium
S102. A second step of separating the yeast component and the precipitate after the supernatant is removed by centrifuging the yeast and the culture solution,
S103. The third step of washing the yeast component of the second stage with distilled water to remove the water-soluble odor component
S104. The fourth step of removing the supernatant by centrifuging the yeast component in the third step
S105. The fifth step of washing the yeast component with the alcohol to remove the alcohol soluble odor component
S106. Step 6) of removing the supernatant by centrifuging the yeast component of step 5
S107. Step 7 in which the yeast component of step 6 is dispersed in distilled water to expand the cells
S108. Step 8 of spreading the yeast cell component and the crushed product in the distilled water by crushing the cells of the yeast component in the step 7,
S109. The ninth step is to centrifuge the distilled water to remove the non-aqueous precipitate, and then collect the aqueous supernatant.
S110. Tenth step of sterilizing the water-soluble supernatant to remove microorganisms that may be decayed
S111. Step 11) of preparing the yeast component extract by freezing or lyophilizing the aqueous supernatant of step 10)

Claims (4)

Wherein the yeast extract is at least one of yeast extract, yeast extract, yeast extract, yeast extract, yeast extract, A step of fermenting yeast in a culture solution;
A second step of separating the yeast component and the precipitate after the supernatant is removed by centrifuging the yeast and the culture solution;
A third step of washing the yeast component of the second step with distilled water to remove the water-soluble odor component;
A fourth step of removing the supernatant by centrifuging the yeast component of step 3;
A fifth step of washing the yeast component of step 4 with alcohol to remove the alcohol soluble odor component;
A sixth step of centrifuging the yeast component of step 5 to remove the supernatant;
A seventh step of expanding the cells by dispersing the yeast component of step 6 in distilled water;
An eighth step of disrupting cells of the yeast component in step 7 to diffuse the yeast cell component and the crushed product in distilled water;
Separating the water-soluble supernatant after removing the non-aqueous precipitate by centrifuging the distilled water in the eighth step;
A step 10 of sterilizing the aqueous supernatant to remove microorganisms that may be decayed;
And a step of preparing a yeast component extract by freezing or freeze-drying the aqueous soluble supernatant of step 10). 3. The method of claim 2,
In the fifth step,
A method for preparing a hair loss therapeutic agent using a yeast component extract characterized by washing the hair with 1 to 3 times of an alcohol at a concentration of 70% 3. The method of claim 2,
Wherein the cell breaking in the eighth step is carried out using at least one of an ultrasonic wave crusher, a microfluidizer, a glass bead, and a French press.

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