KR20150123772A - A composition for the treatment and prevention of gonorrhea or disease induced by Neisseria gonorrhoeae comprising Hexanedioic acid - Google Patents

Abstract

In the present invention, it is revealed that hexanedioic acid has an antibacterial activity or an antibacterial effect against Neisseria gonorrhoeae by inhibiting growth and proliferation of the Neisseria gonorrhoeae and inducing growth inhibition, and can be used as a composition for treating, alleviating, or preventing diseases caused by infection with the Neisseria gonorrhoeae utilizing the same. The present invention relates to a pharmaceutical composition for treating or preventing diseases caused by infection with the Neisseria gonorrhoeae, a health food composition for preventing or alleviating gonorrhea, and a feed composition for preventing or alleviating gonorrhea, containing hexanedioic acid as an active ingredient. The pharmaceutical composition, the health food composition, and the feed composition in accordance with the present invention inhibit growth and proliferation of the Neisseria gonorrhoeae or induce growth inhibition, thereby being useful as a bactericide for treating, alleviating, or preventing diseases caused by infection with the Neisseria gonorrhoeae.

Description

TECHNICAL FIELD The present invention relates to a composition for the treatment and prevention of gonorrhoeae or diseases caused by gonorrhea comprising hexanediol acid, and a composition for preventing or treating gonorrhea or disease,

The present invention relates to a pharmaceutical composition for the treatment or prevention of a disease caused by Neisseria gonorrhoeae comprising hexanedioic acid as an active ingredient, a health food composition for preventing or ameliorating gonorrhea, Or improving feed composition.

Hundreds of years ago, 3 out of 10 children died before they were 1, and about half died before 10 years old. The reason was due to infectious diseases such as smallpox, measles, malaria, cholera, dysentery, pneumonia, sepsis, typhoid and pest. Findings of penicillin during the inhibition and treatment of these diseases have become a turning point that has led to major changes in antibiotic-induced inhibition of pathogens, improvement of health health and prolongation of life by inhibition of disease.

However, direct or indirect damages caused by pathogenic microorganisms and resistant bacteria still cause many problems and diseases in economy, environment, medicine and health. The use of chemical pesticides in agriculture for agricultural products and the use of antibiotics in the aquaculture industry, the harmfulness and environmental pollution of the human body, the antibiotic resistance due to abuse of a wide range of antibiotics The emergence of strains has caused many problems in society as a whole. In particular, recently, microorganisms showing extensive resistance to antibiotics have been discovered and the problem of resistance to antibiotics has become serious, and the research and development and dissemination of new antibiotics are urgently required. In addition, antibiotics generally play a very important role not only in humans but also in animal husbandry. In order to increase the number of rearing per unit area and to improve the productivity, the prevention and treatment of disease was an important factor of the successful axis and fisheries industry in accordance with the development of axis and fisheries including past agriculture. Therefore, various antibiotics and growth promoters have been developed and distributed and applied in the field. This increase in the amount of antibiotics caused resistance and contamination to axes and aquatic products. Furthermore, through the bioconcentration phenomenon, not only does it induce drug resistance to humans consuming and consuming axes and aquatic products, The situation reached.

Most of the currently used antibiotics are produced through chemical synthesis, and thus exhibit many limitations such as resistance, contamination and side effects as described above. Therefore, recently, researches for separating and developing new antimicrobial substances from natural products including animals, plants, microorganisms and various medicinal plants and medicinal plants are being actively carried out, and development of a human-friendly substance capable of replacing antibiotic functions is important It is becoming a challenge. However, research and development of these new antimicrobial substances is not easy. In order to develop a new antimicrobial substance, it is necessary to consider that the antimicrobial spectrum is broad and antimicrobial effect is exerted on existing resistant strains, and that even long-term administration is safe without side effects.

Under these circumstances, the present inventor has made intensive efforts to develop a new antimicrobial substance. As a result, the present inventors have completed the present invention by confirming that "Hexanedioic acid", which is known as a chemical substance, induces a high growth inhibitory action in gonococci and has antimicrobial activity .

It is an object of the present invention to provide a pharmaceutical composition for treating or preventing diseases caused by gonorrhea comprising hexanedioic acid as an active ingredient.

Another object of the present invention is to provide a health food composition for preventing or ameliorating gonorrhea comprising hexane diol acid (Hexanedioic acid) as an active ingredient.

Another object of the present invention is to provide a feed composition for preventing or improving gonorrhea comprising hexane diol acid (Hexanedioic acid) as an active ingredient.

In order to achieve the above object, the present invention provides a pharmaceutical composition for treating or preventing diseases caused by gonorrhea comprising hexanedioic acid as an active ingredient.

The term "hexanedioic acid" used in the present invention is also called "adipic acid" or "hexane-1,6-dioic acid" and is white crystals having the chemical structure C ₆ H ₁₀ O ₄ and the molar mass ) Is 146.14 g / mole. The antimicrobial activity of Hexanedioic acid was measured by dissolving Hexanedioic acid in distilled water. In one embodiment of the present invention, the Hexanedioic acid inhibited the growth and proliferation of gonococci and inhibited the viability of gonads, thereby confirming the growth inhibition or antibacterial activity against gonorrhoeae (FIGS. 1 and 2).

As used herein, the term "gonadal" refers to a disease, also called gonococcal infection, which is caused by infection of gonorrhoeae and propagates through sexual contact. It is common to appear in the form of urethritis in men and cervicitis in women, and it usually occurs in the form of epididymitis, prostatitis, oophoritis, pelvic infection, pharyngitis, rectitis, conjunctivitis, sepsis, arthritis, meningitis and endocarditis Symptoms appear. Gonads that can be prevented or treated with the pharmaceutical composition of the present invention are not limited thereto, but may be preferably gonads caused by Neisseria gonorrhoeae.

As used herein, the term " Neisseria gonorrhoeae "refers to a gram-negative nougat-type microorganism, which is a microorganism that does not have a capillary, flagella, or spore. It is a gonadal pathogen and human infection is mainly caused by sexual intercourse It causes acute inflammation. In men, it causes urethritis, epididymitis, and prostatitis. In women, it causes urethritis, vaginitis, endometritis, salpingitis, and peritonitis. Through the mucous membranes of the genitourinary tract, they enter the bloodstream (blood flow) or the lymphatic system (lymphatic flow) and the bacteria are scattered throughout the body, resulting in endocarditis or arthritis. Gonorrhea is divided into four types of T1, T2, T3 and T4 depending on the characteristics of colonies. The colonies of T1 and T2 are large and dark golden. T2 has a marginal margin of colonization, and platinum is tangible. The colonies of T3 and T4 are large, T3 is pale polychrome, the surface of the colonies is coarse and rough, and T4 is colorless transparent and amorphous.

In the present invention, the therapeutic or preventive effect of the gynecological composition of the present invention may be attained by inhibiting the growth and proliferation of gonads and inhibiting the survival of gonorrhoeae, thereby having an inhibitory effect on growth or antimicrobial activity against gonorrhoeae. In one embodiment of the present invention, it has been confirmed that hexanedioic acid has antimicrobial activity against the gonorrhoeae, thereby achieving the treatment or prevention of gonorrhea (FIGS. 1 and 2).

As used herein, the term " antibacterial " as used herein means an effect against bacteria, more specifically, a bacterial and fungal growth inhibition, proliferative growth inhibition or growth inhibitory action by a drug, a physiologically active substance or a known chemical substance. For the purpose of the invention, it means the growth of gonococci and inhibition of proliferation or inhibition of growth. In one embodiment of the present invention, it was confirmed through the TB assay that the hexanedioic acid had antimicrobial activity against the germ (FIGS. 1 and 2).

The hexanedioic acid of the present invention is not limited thereto, but may be a synthetic material obtained by chemical synthesis or a substance derived from an animal, a plant and an insect. The hexanedioic acid can be obtained by an extraction method or a separation and purification method derived from common synthetic methods, animals, plants and insects known in the art. The extraction method of the extract is not limited thereto, but organic solvent extraction, hot water extraction, hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction may be used.

In the present invention, the solvent used for extracting, extracting, or preparing the hexanedioic acid may be water or organic solvent commonly used in the art. Examples of the solvent include water, hexane, chloroform, Ethanol, methanol, butanol, acetate, acetone, acetonitrile, benzene, dichloromethane, or a mixed solvent thereof may be used as the solvent.

&Quot; Prevention "used in the present invention means all the actions of inhibiting or delaying the onset of the disease upon administration of the composition, and" treatment "means any action that alleviates or alleviates symptoms of the disease upon administration of the composition do.

The pharmaceutical composition of the present invention may comprise a pharmaceutically acceptable carrier. Compositions comprising a pharmaceutically acceptable carrier can be of various oral or parenteral formulations. In the case of formulation, it can be prepared using diluents or excipients such as fillers, extenders, binders, humectants, disintegrants, surfactants and the like which are usually used. Solid formulations for oral administration may include tablet pills, powders, granules, capsules, and the like, which may contain one or more excipients, such as starch, calcium carbonate, sucrose, or lactose lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

The pharmaceutical composition of the present invention may also be in the form of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, Or a pharmaceutically acceptable salt thereof.

The dosage form of the pharmaceutical composition of the present invention is not particularly limited and may be varied depending on the degree of absorption, body weight, age, sex, health condition, diet, administration time, administration method, excretion rate, have. The pharmaceutical composition of the present invention is prepared in consideration of an effective dose range, and the unit dosage formulations thus formulated are classified according to the judgment of the expert who monitors or observes the administration of the drug, if necessary, Or may be administered several times at a predetermined time interval.

In another aspect, the present invention provides a health food composition for preventing or ameliorating gonorrhea comprising hexane diol acid (Hexanedioic acid) as an active ingredient. The health food composition may be achieved by inhibiting the growth and proliferation of gonads and inhibiting the viability of the gonads, thereby having a growth inhibitory effect or antimicrobial activity against the gonococci, as described above.

When the composition of the present invention is used as an additive for a health food, the composition may be added as it is or may be used together with other health food or its components, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed may be appropriately determined depending on the purpose of use, and the amount of the composition of the present invention is not particularly limited.

The types of health foods of the present invention include functional foods, food additives, nutritional supplements, and the like, and there is no particular limitation. Examples of the health food to which the composition can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, soups, drinks, tea, , Alcoholic beverages, and vitamin complexes, and may include foods used as feed for animals, which may include all health foods in a conventional sense. In addition, when the health functional food composition of the present invention is used in the form of a drink, it may contain various sweetening agents, flavoring agents, or natural carbohydrates as additional components such as ordinary beverages. In addition to the above, the health functional food composition of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, , Alcohols, carbonating agents used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks.

In another aspect, the present invention provides a feed composition for preventing or improving gonorrhea comprising hexanedioic acid as an active ingredient. The feed composition may preferably be obtained by inhibiting the growth and proliferation of gonads and inhibiting the viability of the gonads, thereby having an inhibitory effect on the gonads and an antimicrobial activity, as described above.

When the composition of the present invention is used as a feed additive, the composition may be added as it is or may be mixed with other feed ingredients and used appropriately according to a conventional method. The amount of the active ingredient to be mixed may be appropriately determined depending on the purpose of use, and the amount of the composition of the present invention is not particularly limited.

There is no particular limitation on the kind of feed of the present invention. The composition may be supplemented with various kinds of auxiliaries such as amino acids, inorganic salts, vitamins, antioxidants, microbial preparations and the like and crushed or crushed wheat, vegetable protein feeds such as barley and corn, animal protein feeds such as blood, May be used in combination with nutritional supplements, growth promoters, digestion-absorption promoters, and disease prevention agents in addition to the main components such as fat and vegetable fats. The dosage form, method and formulation of the feed can vary and are as described above.

The feed composition of the present invention can be applied to a large number of animal diets, i.e. feeds, including mammals, poultry, fish and crustaceans. It can be used for zoo animals such as elephants, camels, giraffes and monkeys, pets such as dogs and cats, including chickens, ducks, geese, Pigeons, and other commercially available fish and crustaceans such as carp, carp, trout, mullet, and shrimp.

The pharmaceutical compositions, health food compositions and feed compositions according to the present invention inhibit the growth and proliferation of gonococci and inhibit survival, thereby inhibiting the growth of gonorrhoeae or antimicrobial activity thereof, thereby treating or improving diseases caused by gonorrhea Or antimicrobial agents that can be usefully used for prevention.

FIG. 1 is a graph showing antimicrobial activity or antimicrobial activity of hexanedioic acid according to the concentration of Hexanedioic acid applied to the TB assay to inhibit the growth and proliferation inhibition or growth inhibition of germ cells. The "+" indicates the growth-inhibiting action or the antibacterial effect of the test substance on the gonorrhoeae.
FIG. 2 is a graph showing the antimicrobial activity of Hexanedioic acid according to the concentration of Hexanedioic acid applied to the TB assay to inhibit the growth and proliferation inhibition or growth inhibition of germ cells.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example  One: TB assay Measurement of antimicrobial activity of test substance by

To determine the antimicrobial activity or antimicrobial activity of Hexanedioic acid against gonorrhea, Hexanedioic acid and distilled water were used to prepare Hexanedioic acid solution at each concentration and then applied to the TB assay to measure the antimicrobial activity of the test substance against gonorrhea.

The TB assay procedure is as follows. First, the LB (Luria-Bertani broth) liquid medium was placed in a glass flask or bottle, and then the test strain was inoculated in a LB (Luria-Bertani broth) liquid medium and cultured in a 37 ° C incubator for 24 hours. Thereafter, 150 μl of the test strain was added to each well of a 96-well plate, and the test solution was administered at the concentration. After 12 hours, the absorbance of each strain was measured and the test solution was not administered The percent antimicrobial activity was calculated by comparing the absorbance of the normal culture with the absorbance.

As a result, it was confirmed through the TB assay experiment that the test substance Hexanedioic acid had a high growth inhibitory activity or antimicrobial activity in a concentration-dependent manner on the gonococci (FIGS. 1 and 2). These results indicate that hexanedioic acid can be used as an antimicrobial composition against gonorrhoeae.

Claims (5)

A pharmaceutical composition for treating or preventing a disease caused by Neisseria gonorrhoeae comprising hexanedioic acid as an active ingredient.
The pharmaceutical composition according to claim 1, wherein the composition induces growth inhibition of gonococci.
The method of claim 1, wherein the disease caused by the gonorrhea is at least one selected from the group consisting of gonorrhea, urethritis, epididymitis, prostatitis, cervicitis, ovarianositis, pelvic infection, pharyngitis, rectalitis, conjunctivitis, arthritis, meningitis and endocarditis ≪ / RTI >
A health food composition for preventing or ameliorating gonorrhea comprising hexanedioic acid as an active ingredient.
A feed composition for preventing or improving gonorrhea comprising hexanedioic acid as an active ingredient.

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