KR20150121492A - Composition for skin cell regeneration or anti-wrinkle - Google Patents

Abstract

The present invention provides a composition for regenerating skin and/or reducing wrinkles, comprising dapnetin or pharmaceutically acceptable salts thereof and, particularly, a cosmetic and pharmaceutical composition. The cosmetic and pharmaceutical compositions of the present invention are safe to a human body due to reduced side effects, while having excellent effects in regenerating skin and reducing wrinkles.

Description

Technical Field [0001] The present invention relates to a composition for skin regeneration or anti-wrinkle,

The present invention relates to a composition, particularly a cosmetic composition and a pharmaceutical composition, related to skin regeneration and / or wrinkle improvement, and more particularly to a cosmetic composition and a pharmaceutical composition which are excellent in skin regeneration and wrinkle reducing effect even in small amounts.

Collagen is a major substrate protein produced in fibroblasts of the skin and exists in extracellular epilepsy. Its important functions are mechanical rigidity of skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, division of cells and differentiation Growth or wound healing) are known. Such collagen is reduced by aging and photo aging caused by ultraviolet irradiation, which is known to be closely related to the wrinkling of the skin. Also, in recent years, extensive research on skin aging has developed, and important functions of collagen in skin have been revealed.

Effective ingredients promoting collagen synthesis and exhibiting wrinkle-reducing effects are known. For example, retinoic acid, transforming growth factor (TGF), animal placenta-derived protein, betulinic acid, and chlorella extract are known as collagen synthesis promoting substances. However, the above-mentioned effective ingredients are limited in the use amount due to safety problems such as irritation and redness when applied to the skin, or have insufficient effect, so that the effect of improving the skin function by promoting the collagen synthesis of the skin can not be expected.

Therefore, it is urgently required to develop a composition having a skin regeneration and wrinkle-reducing effect that is more safe than a living body, has an effective ingredient stable, and is more effective than a substance having an effect of regenerating skin and improving wrinkles.

Accordingly, the present invention is to provide such a useful use of a new active ingredient, that is, an active ingredient, which solves the above problems and is safe and safe for the human body and has excellent skin regeneration and / or wrinkle-reducing effects.

In other words, a problem to be solved by the present invention is to provide a composition comprising the active ingredient having excellent efficacy as an active ingredient.

In order to solve the above problems, the present invention provides a composition for improving skin regeneration and / or wrinkling, preferably a cosmetic composition and a pharmaceutical composition, comprising daphnein or a pharmaceutically acceptable salt thereof as an active ingredient .

That is, the present invention provides a new use for dermal renewal and wrinkle improvement of daphnetin or a pharmaceutically acceptable salt thereof.

The inventors of the present invention have found that daphnetin or a pharmaceutically acceptable salt thereof promotes collagen synthesis of fibroblasts of the skin and inhibits collagenase activity thereby promoting regeneration of skin and improving wrinkles And completed the present invention.

In the present invention, the term " skin regeneration effect " refers to restoration of skin tissue against damage caused by external or internal causes of the skin. Damage due to external causes may include ultraviolet rays, external contaminants, wound, trauma, etc. The damage caused by the internal causes may be stress.

In the present invention, the term " wrinkle-reducing effect " refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already-generated wrinkles.

In order to exert an excellent effect when the skin regeneration and wrinkle improving ingredient is applied to actual skin, it exhibits high activity of skin regeneration and wrinkle reducing activity at a low concentration, has excellent ability to permeate and absorb the skin, Is low in volatility so as to be able to stay for a sufficient time to be shown, and is stable on the composition or on the skin, is easy to be formulated into medicines or cosmetics, and is safe for the skin. However, the components satisfying all of the above-mentioned characteristics among the known components are not common. For example, some skin regeneration and wrinkle improving agents have excellent skin regeneration and wrinkle reducing activity even at a low concentration in an in vitro test, but they are difficult to be applied to real skin because of their ability to be absorbed through the skin. Other active ingredients have low hydrophilicity and are difficult to formulate into medicines or cosmetics. In addition, some skin regrowth and wrinkle remedying ingredients may be degraded or transformed into other compounds when exposed to heat, light, or oxygen, and the effect may vanish prior to application to the skin.

In order to solve the above-mentioned problems and to achieve the object of the present invention, the present invention provides a composition comprising a compound represented by the following formula (I) or a pharmaceutically acceptable salt thereof.

[Chemical Formula 1]

The compound represented by the formula (1) is referred to as daphnein, and may include all possible isomers.

The present invention is not particularly limited to the method for obtaining daphenetin, and may be chemically synthesized by a method known in the art, or a commercially available substance may be used.

The term "pharmaceutically acceptable salts " of the present invention refers to salts prepared with little or no toxicity or with acids or bases. When the compound of the present invention is relatively acidic, the base addition salts may be obtained by contacting a neutral form of such compound with a sufficient amount of the desired base and an appropriate inert solvent. Pharmaceutically acceptable base addition salts include, but are not limited to, salts formed with sodium, potassium, calcium, ammonium, magnesium or organic amino. Where the compounds of the present invention are relatively basic, acid addition salts can be obtained by contacting neutral forms of such compounds with a sufficient amount of the desired acid and a suitable inert solvent. Where the compounds of the present invention are relatively basic, acid addition salts can be obtained by contacting neutral forms of such compounds with a sufficient amount of the desired acid and a suitable inert solvent. Pharmaceutically acceptable acid addition salts include those derived from organic acids such as propionic acid, isobutyric acid, oxalic acid, malic acid, malonic acid, benzoic acid, succinic acid, sueric, fumaric acid, mandelic acid, phthalic acid, benzenesulfonic acid, Phosphoric acid, monohydrogencarbonic acid, phosphoric acid, monohydrogenphosphoric acid, dihydrogenphosphoric acid, sulfuric acid, monohydrogensulfuric acid, hydrogen iodide, phosphorous acid, And the like, but are not limited thereto. But are not limited to, salts of amino acids such as arginate and analogs of organic acids such as glucuronic or galactunoric acids. The above-mentioned acid addition salts may be obtained by a) directly mixing the compound of formula 1 and an acid, or b) dissolving and mixing one of them in a solvent or a water solvent, or c) Lt; RTI ID = 0.0 > acid < / RTI > in a solvent and mixing them.

In addition to the above, additionally saltable forms include, but are not limited to, the salts of gabapentin, pregabalin, nicotinate, adipate, hemimarate, cysteine, acetylcysteine, methionine, arginine, Aspartate and the like.

Daphenetin of the present invention may exist in unsolvated form as well as solvated form including form of hydrate, ethanol, and the like. The daphnetin of the present invention may exist in crystalline or amorphous form, and all such physical forms are included within the scope of the present invention.

When the compound of formula (I) or a pharmaceutically acceptable salt thereof of the present invention is used as a medicament, it may further contain one or more active ingredients exhibiting the same or similar functions. For example, it may contain known skin regeneration and wrinkle improving ingredients. The addition of additional skin regeneration and wrinkle improving ingredients may further enhance skin regeneration and wrinkle reducing effects of the composition of the present invention. When the above ingredients are added, skin safety, easiness of formulation, and stability of effective ingredients can be considered according to the combined use. In one embodiment of the invention, the composition is comprised of retinoic acid, TGF, protein from animal placenta, betulinic acid and chlorella extract, derivatives thereof and various plant extracts as skin regeneration or wrinkle improving ingredients known in the art And one or more components selected from the group consisting of The additional component may be contained in an amount of 0.0001 wt% to 10 wt% based on the total weight of the composition. For example, from 0.0001 wt% to 1 wt%, from 0.0001 wt% to 0.1 wt%, from 0.0001 wt% to 0.001 wt%, from 0.001 wt% to 10 wt%, from 0.001 wt% 0.001 wt% or more to 0.1 wt% or less, 0.01 wt% or more to 10 wt% or less, or 0.01 wt% or more to 1 wt% or less. The content range may be adjusted according to requirements such as skin safety, ease of formulation of the compound of Formula 1 or the salt of the compound.

In addition, the pharmaceutical composition for skin regeneration and wrinkle improvement of the present invention may further comprise a pharmaceutically acceptable carrier.

Pharmaceutically acceptable carriers may contain a variety of ingredients such as buffer, injectable sterile water, normal saline or phosphate buffered saline, sucrose, histidine, salts and polysorbates.

The pharmaceutical composition of the present invention can be administered orally or parenterally, and can be administered in the form of a general pharmaceutical preparation, for example, various forms of oral and parenteral administration at the time of clinical administration. In the case of formulation, , An extender, a binder, a wetting agent, a disintegrant, a surfactant, and the like.

Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient such as starch, calcium carbonate, Sucrose, lactose, gelatin, and the like.

In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral administration include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included .

Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of suppository bases include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like.

The pharmaceutical composition of the present invention can provide a desirable skin regeneration effect and wrinkle-reducing effect when an effective amount of the compound of Formula 1 or a pharmaceutically acceptable salt thereof is contained. In the present invention, the term "effective amount" means the amount of a compound capable of promoting regeneration of damaged skin or exhibiting a wrinkle-improving effect. An effective amount of the compound of formula (1) contained in the composition of the present invention will vary depending on the form in which the composition is commercialized, how the compound is applied to the skin, and the time on the skin. For example, when the composition is commercialized as a pharmaceutical product, the compound of Formula 1 may be contained at a higher concentration than that of a cosmetic product that is routinely applied to skin. Accordingly, the daily dosage is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, based on the amount of the compound of formula (1) 6 times a day.

The pharmaceutical composition of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.

The present invention can also be provided as a formulation for external skin preparation for skin regeneration and wrinkle improvement, which comprises the compound of formula (I) or a pharmaceutically acceptable salt thereof as an active ingredient.

When the compound of formula (1) is used as an external preparation for skin, it may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, , Water, ionic or nonionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or external preparations for skin And any other ingredients used in the skin sciences. The components can also be introduced in amounts commonly used in the field of dermatology.

When the compound of Formula 1 is provided as an external preparation for skin, it may have a formulation such as, but not limited to, ointments, patches, gels, creams or sprays.

The present invention can also be provided as a formulation of a cosmetic for regenerating skin and improving wrinkles containing the compound of formula (I) or a pharmaceutically acceptable salt thereof as an active ingredient.

When the compound of Chemical Formula 1 is used as a cosmetic, the cosmetic product containing the compound of Chemical Formula 1 as an active ingredient may be prepared in the form of a general emulsified formulation and a solubilized formulation. For example, creams, essences, cosmetic creams, sprays, gels, packs, sunscreens, make-up bases, liquids such as lotions such as lotion, facial lotion, body lotion, A powder, a cleansing lotion, a makeup removing agent such as a cleansing oil, a cleaning agent such as a cleansing foam, a soap, a body wash and the like.

The cosmetic composition may further contain, in addition to the compound of Formula 1, a lipid, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, , Ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And may contain adjuvants conventionally used in the cosmetics field, such as any other ingredient.

In the case of a wash-off type cosmetic such as a make-up removing agent, a detergent, etc. in which the active ingredient remains on the skin in a short period of time when the compound of formula (1) is produced into a cosmetic product, . On the other hand, in the case of leave-on type cosmetics, such as lotion, cream, essence, etc., in which the active ingredient remains on the skin for a long period of time, It may be included. In one embodiment of the present invention, the composition is used in an amount of 0.000001 wt% to 10 wt%, preferably 0.001 wt% to 10 wt%, more preferably 0.001 wt% to 10 wt% May be 0.1 to 10% by weight. When the composition of the present invention contains less than 0.000001% by weight of the compound of the formula 1, sufficient skin regeneration or wrinkle-reducing effect can not be expected. When the composition contains more than 10% by weight, This is to prevent skin safety problems.

The present invention also relates to a health food for skin regeneration or wrinkle improvement comprising the compound of formula (I) or a pharmaceutically acceptable salt thereof.

The term 'health food' as used herein means a food prepared by adding the compound of formula (1) to food materials such as beverage, tea, spice, gum and confectionery, or by encapsulation, powdering or suspension, But it has the advantage that there are no side effects that can occur when a drug is used for a long period of time using a food as a raw material.

Since the health food of the present invention thus obtained can be taken on a daily basis, a high skin regeneration or wrinkle reducing effect can be expected, which is very useful.

When the compound of Chemical Formula 1 is used as a food additive, the compound of Chemical Formula 1 may be added as it is or may be used together with other food or food ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, when the food or beverage is produced. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of the food to which the above substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include healthy foods in a conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

In addition to the above, the health food of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, , A carbonating agent used in carbonated drinks, and the like. In addition, the health food of the present invention may contain flesh for the production of natural fruit juice, fruit juice drink and vegetable drink. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The composition containing the daphnetin of the present invention or a pharmaceutically acceptable salt thereof exhibits a skin regeneration and / or wrinkle-reducing effect.

The composition containing the daphnetin or a pharmaceutically acceptable salt thereof of the present invention can be used as a safe, excellent cosmetic raw material, a pharmaceutical ingredient, and a health functional food raw material.

FIG. 1 is a graph showing the collagen growth rates of the compound of Chemical Formula 1 and the control group, respectively. FIG.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. The embodiments of the present invention are provided by way of example to facilitate a specific understanding of the present invention.

Reference Example  One: Daphnetin's  Material Information

Daphnetin

CAS No .: 486-35-1

Where to buy: Tokyo Chemical Industry Co. Ltd.

Experimental Example  1: Increase effect of total collagen

The compound of Formula 1 was added to the culture medium of human-derived fibroblasts and the total amount of collagen was measured at the cellular level. The total amount of synthesized collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay Kit).

To evaluate the cytotoxicity of human fibroblasts before experimentation at concentrations of 10 ppm, 1 ppm, 0.1 ppm, 0.01 ppm, and 0.001 ppm of the test substance (method for MTT test by culturing fibroblasts [Reference: Mossman T. ), And the total amount of collagen was measured by selecting a concentration without cytotoxicity. The results are shown in Table 1.

In the experiment, the final concentrations of the compound of Chemical Formula 1 were set to 0.1 ppm and 1 ppm, respectively. Each sample was added to the culture medium of human fibroblasts and cultured for 1 day. Then, the culture broth was taken and the collagen The total amount was measured at 450 nm using a spectrophotometer. For the comparison of the effects, the final concentration of scopoletin and the final concentration of fibroblast culture medium (control group) were set at 0.1 ppm and 1 ppm, respectively. TGFb was added to the final concentration of 10 ppm for the total amount of collagen Respectively. The total amount of collagen was measured as a UV absorbance, and the rate of increase of the total amount of collagen was calculated by the ratio of the total amount of collagen relative to the control, and the results are summarized in Table 1 below.

Increased effect of total collagen (number of repeats = 6) sample Absorbance average Collagen growth rate (%) The compound of formula (1)
(0.1 ppm) 588 129 The compound of formula (1)
(1 ppm) 702 154 Control group
(No additives) 456 - Scopoletin
(0.1 ppm) 538 118 Scopoletin
(1 ppm) 547 120 TGFb
(10 ppm) 597 131

As can be seen from the results in Table 1, the compound (1 ppm) of formula (1) exhibited an excellent collagen growth rate at a concentration lower than that of TGFb (10 ppm) known to have collagen synthesis ability and showed a much higher activity than Scopoletin having a similar structure . Therefore, it was found that the compound of Chemical Formula 1 can be used for skin regeneration and wrinkle improvement.

Manufacturing example  1 and Comparative Example  One: Ointment for external skin Prescription example

Composition Production Example 1 Comparative Example 1 The compound of formula (1) 0.5 - Diethyl sebacate 8 8 Nonsense 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Suitable amount Suitable amount vaseline to 100 to 100

As shown in Table 2, an external skin ointment containing the compound of Chemical Formula 1 as an active ingredient was prepared according to a conventional method. The content of Table 2 was expressed in% by weight.

Manufacturing example  2 and Comparative Example  2: Cosmetics  Of creamy Prescription example

Composition Production Example 2 Comparative Example 2 The compound of formula (1) 0.2 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water to 100 to 100

As shown in Table 3, a cream containing the compound of Chemical Formula 1 as an active ingredient was prepared by a conventional method. The contents of Table 3 are expressed as% by weight.

Manufacturing example  3 and Comparative Example  3: Cosmetics  Of flexible lotion Prescription example

Composition Production Example 3 Comparative Example 3 The compound of formula (1) 0.1 - ethanol 10.0 10.0 Polyoxyethylene sorbitan polylauric acid 1.0 1.0 Paraoxybenzoic acid methyl 0.2 0.2 glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water to 100 to 100

Lotion containing the compound of the formula (1) as an active ingredient as shown in Table 4 was prepared according to a conventional method. The content of Table 4 is expressed in% by weight.

Manufacturing example  4 and Comparative Example  4: Cosmetics  Of nutritional lotion Prescription example

Composition Production Example 4 Comparative Example 4 The compound of formula (1) 0.05 - Vaseline 2.0 2.0 Sesquioleic acid sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Paraoxybenzoic acid methyl Suitable amount Suitable amount Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxyvinyl polymer 18.0 18.0 Potassium hydroxide 0.1 0.1 Pigment Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water to 100 to 100

As shown in Table 5, a lotion containing the compound of Chemical Formula 1 as an active ingredient was prepared by a conventional method. The contents in Table 5 are expressed in weight percent.

Manufacturing example  5 and Comparative Example  5: Cosmetics  Pack of Prescription example

Composition Production Example 5 Comparative Example 5 The compound of formula (1) 0.05 - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleyl ether 1.0 1.0 Paraoxybenzoic acid methyl 0.2 0.2 Pigment Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water to 100 to 100

As shown in Table 6, a pack containing the compound of Chemical Formula 1 as an active ingredient was prepared according to a conventional method. The content of Table 6 was expressed in weight%.

Manufacturing example  6 and Comparative Example  6: Cosmetics  Of the essence Prescription example

Composition Production Example 6 Comparative Example 6 The compound of formula (1) 0.2 - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyaluronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene hardened castor oil 1.0 1.0 Paraoxybenzoic acid methyl 0.1 0.1 incense Suitable amount Suitable amount Purified water to 100 to 100

Essences containing the compound of Chemical Formula 1 as an active ingredient were prepared according to a conventional method as shown in Table 7 above. The contents of Table 7 are expressed in% by weight.

Experimental Example  3

The effect of improving the wrinkles was tested in healthy 35 to 50 year old women against the essence of the above-described Production Example 6 and Comparative Example 6. [

30 women aged 35 to 50 years were divided into two groups by 15 persons. Group 1 contained the essence of Preparation Example 6 containing 0.2% of the compound of Formula 1, Group 2 contained the essence of Comparative Example 6, 2 times a day for 3 months.

After 3 months, the improvement of wrinkles was evaluated by image analysis of questionnaires and wrinkles. The subjects' questionnaires were compared with those before use with respect to wrinkle improvement and elasticity improvement. No improvement, slight improvement, and significant improvement were determined. The results are shown in Table 8.

Wrinkle improvement effect (persons) sample No improvement Slight improvement A significant improvement Comparative Example 6 11 4 0 Production Example 6 - 10 5

As can be seen from the above Table 8, it can be seen that the wrinkle improving effect is excellent when the preparation example 6 according to the present invention is used.

Immediately after the experiment, the replica was collected from the same area under the eye. The image was analyzed by image analysis to determine the degree of wrinkles The density of wrinkles was measured by dimensional analysis. The measurement results of the wrinkle density by the image analysis are shown in Table 9 below by averaging the reduction rates with respect to the wrinkle density before use.

Wrinkle improvement effect sample Wrinkle density reduction rate (%) Comparative Example 6 8% Production Example 6 42%

As can be seen from Table 9, when the preparation example 6 prepared by adding the compound of formula 1 according to the present invention was applied, the density of the wrinkles was significantly decreased before use.

Claims (3)

A cosmetic composition for skin regeneration or wrinkle improvement comprising a compound represented by the following formula (1) or a pharmaceutically acceptable salt thereof as an active ingredient.
[Chemical Formula 1]

A pharmaceutical composition for skin regeneration or wrinkle improvement comprising a compound represented by the following formula (1) or a pharmaceutically acceptable salt thereof as an active ingredient.
[Chemical Formula 1]

The composition according to claim 1 or 2, wherein the compound represented by the formula (1) or a pharmaceutically acceptable salt thereof is contained in an amount of 0.0001 to 10% by weight based on the total weight of the composition.

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