KR20150113717A - Compositions for Improving Skin Aging Comprising of Allium hookeri Extract - Google Patents

Abstract

The present invention relates to a novel use of an Allium hookeri extract and, more specifically, to a composition for wrinkle amelioration and anti-aging containing an Allium hookeri extract. The Allium hookeri extract inhibits degradation of collagen, stimulates collagen synthesis, and thus exhibits excellent anti-aging activities and outstanding effects of ameliorating, preventing, or treating wrinkles, thereby being used as an active ingredient in a cosmetic composition, a food composition, or a pharmaceutical composition.

Description

Technical Field [0001] The present invention relates to a composition for improving skin aging,

The present invention relates to a process for the preparation of Allium hookeri ) extracts. More specifically, the present invention relates to a composition for improving wrinkles or an anti-aging composition, which comprises a triple-layered extract.

      Skin protects the organs in the body from external stimuli and plays an important role in the maintenance of body homeostasis, such as body temperature control. Such skin is aged by various internal and external factors and is divided into endogenous aging by genetic cause and exogenous aging by environmental cause. Among them, photoaging refers to aging by ultraviolet (UV). Recently, the destruction of the ozone layer due to environmental pollution has increased the amount of ultraviolet rays, and accordingly, research on photoaging has been attracting attention (Wang SQ et al., Dermatol. Ther. 23 (1): 31-47, 2010). In the photoaged skin, appearance characteristics such as roughness, loss of elasticity, wrinkles and irregular pigmentation are observed. Among them, the main research field of photoaging is the change of the skin wrinkles. There have been many reports on the basic physiological metabolism changes such as synthesis, degradation, and moisture content of collagen, which is a major constituent of skin, in the formation of skin wrinkles by photoaging (Brenneisen et al., Ann. NY Acad. Sci., 973: 31-43, 2002).

      Collagen in the dermal layer of the skin occupies about 70-80% of the total dry weight of the skin and it is known to control the elasticity of the skin along with the elastic fiber elastin. In particular, ultraviolet rays increase the production of reactive oxygen species, collapse of enzymatic and non-enzymatic antioxidant defenses of the skin, and collagen degradation and biosynthesis are reduced, resulting in a significant reduction of collagen in the dermis (Bickers DR , Athar M, J. Invest. Dermatol., 126 (12): 2565-2575, 2006). Important collagen degradation factors are matrix metalloproteinases (MMPs), which are involved in the degradation of extracellular matrix and basement membrane. Studies have been reported that the enzyme increases activity by ultraviolet rays and inhibits it, leading to an increase in skin thickness and wrinkle formation induced by ultraviolet light (Inomata S et al., J. Invest. Dermatol., 120 ): 128-134, 2003). Therefore, it is effective to control MMPs for prevention and treatment of photoaging.

      Hyaluronic acid, a component of the skin's dermis layer, is a component that keeps the skin moisturized and elastic by filling the collagen between the dermis layers. In particular, ultraviolet light reduces the amount of hyaluronic acid to reduce moisture content in the skin and increase the amount of transdermal water loss, resulting in damage to the skin barrier. This results in rough skin and reduced elasticity, resulting in wrinkles (Baumann L, J. Pathol., 211 (2): 241-251, 2007). That is, loss of moisture of the skin is a main cause of skin aging.

      Consumers' expectation and interest in wrinkle improvement has been diversified from retinol to natural products extract, adenosine, and cell growth factor from the 2000s (Lee EJ et al., Journal of the Korean Society of Cosmetology, 17 (1): 127-133, 2011). Conventional methods are to manufacture and use cosmetic products or medicines containing retinoid, ascorbic acid, tocopherol, hyaluronic acid and the like. However, the active ingredients for the improvement of skin wrinkles or anti-aging currently being developed can not be used as cosmetic raw materials or are very unstable and are difficult to deliver to the skin. Therefore, a special stabilization system and delivery system are required. There is a problem that it is not visible. The retinoid, which is an active ingredient for improving the wrinkles of the skin, is used for inhibiting collagenase and increasing collagen synthesis, thereby improving photoaging phenomenon such as wrinkle formation and reduction in elasticity. However, since it is very sensitive to ultraviolet rays, it easily causes a chemical change, which causes adverse effects such as skin irritation and long-term use (Rabe JH, J. Am. Acad. Dermatol., 55: 1-19, 2006). In order to solve the problems of the above-mentioned active ingredients, there is a demand for studies on active ingredients derived from natural products which have proved to be stable.

Allium hookeri is called Root Leek. In Korea, it is called "三 菜" because it has sweetness, bitter taste, and pungent flavor. Sanjay is native to the Himalayas above 1,400 meters above sea level and is distributed in China, India, Bhutan, Sri Lanka and Myanmar. In particular, Myanmar is being used as a food and medicine for various inflammatory diseases and cancer diseases by a private prescription. Protein, sugar, fiber, ascorbic acid, phytosterol, total phenol, etc. are contained more than onions, and dietary sulfur compounds are known to be 6 times more than garlic. Inhibition of DPPH radical scavenging activity and nitric oxide (NO) production (J. Soc. Food Sci. Nutr. 41: 1645-1648, 2012), anti-inflammatory effects in macrophages induced by lipopolysaccharide (J. Korean Soc. Food Sci. Nutr. 42: 1351-1356, 2013). However, there has been no report on wrinkle improvement or anti-aging effect of the tubular extract.

      Accordingly, the inventors of the present invention have studied various plants to find a plant having a skin aging activity derived from natural products. As a result, it has been found that the tea extract inhibits collagen degradation and promotes collagen production, The present invention has been completed.

      Accordingly, an object of the present invention is to provide a cosmetic composition for improving wrinkles and anti-aging characterized by containing a three-component extract.

      Another object of the present invention is to provide a food composition for improving wrinkles and anti-aging characterized by containing a tangerine-rich extract.

Another object of the present invention is to provide a pharmaceutical composition for improving wrinkles and anti-aging characterized by containing a triple extract.

      In order to achieve the above object, the present invention provides a cosmetic, food and pharmaceutical composition for improving wrinkles and anti-aging characterized by containing a truffle extract.

      Hereinafter, the contents of the present invention will be described in more detail.

      The present invention is not limited to a method for producing a tubular extract, but is preferably a method for producing a tubular extract from water or a part (leaf or root) of a tubular plant or a plant, an organic solvent having 1 to 6 carbon atoms and a subcritical or supercritical fluid Or more. It can also be prepared, if necessary, in addition to filtration and concentration steps according to methods known to those skilled in the art. In addition, specific fractions enriched in active material can be prepared using techniques such as solvent fractionation, silica gel chromatography, and prep-HPLC.

      The organic solvent having a carbon number of 1 to 6 is not limited thereto, but preferably an alcohol having 1 to 6 carbon atoms, acetone, ether, benzene, chloroform, ethyl (ethyl) acetate, methylene chloride, hexane, cyclohexane, and petroleum ether.

      Preferably, the tubular extract of the present invention can be obtained by extracting and drying the dried triangle using purified water, ethanol and subcritical carbon dioxide suitable for food processing, or extracting and purifying it using an ultra-high pressure extraction apparatus Or can be obtained by separating and purifying the oil obtained by directly pressing the triple plant.

      In one embodiment of the present invention, an extract was prepared using a root and leaf (see Example 1).

      In another embodiment of the present invention, the production of collagenase-1 by ultraviolet light is promoted in human fibroblasts, and then whether or not the inhibitor of collagenase-1 is inhibited by administering the extract of the present invention is measured. As a result, it was confirmed that the tubular extract of the present invention effectively inhibited the production of collagenolytic enzyme-1 (see Example 2).

      In another embodiment of the present invention, human fibroblasts are irradiated with ultraviolet rays, and then incubated with the extract of the present invention to measure collagen production level. As a result, it was confirmed that when the extract of the present invention was administered, the effect of stimulating collagen production inhibited by ultraviolet irradiation was excellent (see Example 3).

      Therefore, the tubular extract of the present invention inhibits collagen degradation and is excellent in promoting collagen synthesis.

      Increased activity of collagenase and collagen collapse, which is a major component of skin, cause wrinkles, accelerate aging, and reduce skin elasticity. By inhibiting such collagenolytic enzymes and promoting collagen production, the composition of the present invention inhibits wrinkle formation, suppresses aging, and promotes skin elasticity. As described above, the tubular extract of the present invention can be applied to cosmetic compositions for improving wrinkles and anti-aging, foods, dietary supplements, medicines and the like.

      Accordingly, the present invention provides a cosmetic, food, and pharmaceutical composition for wrinkle-improving and anti-aging comprising the extract of the present invention.

      The cosmetic composition of the present invention contains an extract of Tricholoma mucilus as an active ingredient,

(Foundation, lipstick, mascara, make-up base), hair product composition (such as cosmetics, creams, essences, cleansing foams and cleansing water), together with pharmaceutically acceptable excipients Shampoo, rinse, hair conditioner, hair gel) and soap.

      Such excipients include, but are not limited to, emollients, skin penetration enhancers, colorants, perfumes, emulsifiers, thickeners and solvents. In addition, it may further contain flavors, pigments, bactericides, antioxidants, preservatives, moisturizers and the like, and may include thickeners, inorganic salts and synthetic polymeric substances for the purpose of improving physical properties. example

For example, when the cleanser and the soap are prepared with the cosmetic composition of the present invention, the above-mentioned Snookberry extract can be easily added to a common cleanser and soap base. In the case of producing a cream, it can be prepared by adding the above-mentioned triple extract or its salt to a cream base of a typical underwater type (O / W). A synthetic or natural material such as a flavor, a chelating agent, a coloring matter, an antioxidant, an antiseptic, and a protein, a mineral, and a vitamin for the purpose of improving a physical property may be further added.

      The content of the tubular extract contained in the cosmetic composition of the present invention is not limited thereto, but is preferably 0.001 to 10% by weight, more preferably 0.01 to 5% by weight, based on the total weight of the total composition. If the content is less than 0.001% by weight, the desired anti-aging or wrinkle-reducing effect can not be expected. If the content is more than 10% by weight, safety or formability may be difficult.

      Meanwhile, the food composition of the present invention includes all forms of functional foods, nutritionalsupplement, health food, and food additives.

      Food compositions of this type may be prepared in a variety of forms according to conventional methods known in the art. For example, the health food may be prepared by liquefying, granulating, encapsulating, and pulverizing the triple-pleasure extract itself in the form of tea, juice, and drink so that it can be consumed. In addition, the above-mentioned triple extract and a known active ingredient known to have anti-aging or anti-wrinkle effect may be mixed together to prepare a composition. Functional foods may also include but are not limited to beverages (including alcoholic beverages), fruits and processed foods thereof (e.g., canned fruits, jars, jam, maal marred), fish, meat and processed foods (Eg butter, cheeses, etc.), edible vegetable oils (eg, sourdoughs, etc.), breads and noodles (eg udon, buckwheat noodles, ramen noodles, spaghetti, macaroni, , Margarine, vegetable protein, retort food, frozen food, various seasonings (eg, soybean paste, soy sauce, sauces, etc.). In addition, in order to use the above-mentioned tubular extract as a food additive, it may be used in the form of a powder or a concentrate.

      In the food composition of the present invention, the preferable content of the above-mentioned tubular extract is not particularly limited, but is preferably 0.01 to 50% by weight in the finally prepared food. More preferably, the food composition of the present invention may be prepared in the form of a health food, especially when mixed with an active ingredient known to be effective for anti-aging or wrinkle reduction.

      Meanwhile, the pharmaceutical composition of the present invention may further contain the above-mentioned triple extract alone or in combination with one or more pharmaceutically acceptable carriers, excipients or diluents.

      The pharmaceutically acceptable carrier may further include, for example, a carrier for oral administration or a carrier for parenteral administration. Carriers for oral administration may include lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, and the like. The carrier for parenteral administration may also contain water, suitable oils, saline, aqueous glucose and glycols, and may further contain a stabilizer and a preservative. Suitable stabilizers include antioxidants such as sodium hydrogen sulfite, sodium sulfite or ascorbic acid. Suitable preservatives include benzalkonium chloride, methyl- or propyl-paraben and chlorobutanol. Other pharmaceutically acceptable carriers can be found in Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, Pa., 1995).

      The pharmaceutical composition of the present invention can be administered to mammals including humans by any method. For example, it can be administered orally or parenterally. Parenteral administration methods include, but are not limited to, intravenous, intramuscular, intraarterial, intramedullary, intrathecal, intracardiac, transdermal, subcutaneous, intraperitoneal, intranasal, enteral, topical, sublingual or rectal administration Lt; / RTI > The pharmaceutical composition of the present invention may be formulated into oral or parenteral dosage forms according to the route of administration as described above. In the case of a preparation for oral administration, the composition of the present invention may be formulated into a powder, a granule, a tablet, a pill, a sugar, a tablet, a liquid, a gel, a syrup, a slurry, . For example, an oral preparation can be obtained by combining the active ingredient with a solid excipient, then milling it, adding suitable auxiliaries, and then processing the mixture into a granular mixture. Examples of suitable excipients include sugars including lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol and maltitol, and starches including corn starch, wheat starch, rice starch and potato starch, Cellulose such as methylcellulose, sodium carboxymethylcellulose and hydroxypropylmethyl-cellulose and the like, fillers such as gelatin, polyvinylpyrrolidone and the like. In addition, crosslinked polyvinylpyrrolidone, agar, alginic acid or sodium alginate may optionally be added as a disintegrant. Further, the pharmaceutical composition of the present invention may further comprise an anti-coagulant, a lubricant, a wetting agent, a flavoring agent, an emulsifying agent and an antiseptic agent. In the case of a preparation for parenteral administration, it can be formulated by a method known in the art in the form of injection, cream, lotion, external ointment, oil, moisturizer, gel, aerosol and nasal aspirate. These formulations are described in Remington's Pharmaceutical Science, 15th edition, 1975. Mack Publishing Company, Easton, Pennsylvania 18042, Chapter 87: Blaug, Seymour, a commonly known formulary for all pharmaceutical chemistries.

The total effective amount of the pharmaceutical composition of the present invention may be administered to a patient in a single dose and may be administered by a fractionated treatment protocol administered over a prolonged period of time in multiple doses . The pharmaceutical composition of the present invention may vary in the content of the active ingredient depending on the degree of the disease. In the case of parenteral administration, it is preferably administered in an amount of 0.01 to 50 mg, more preferably 0.1 to 30 mg per 1 kg of body weight per day on the basis of the above-mentioned tubercle leaf extract, and in the case of oral administration, To be administered in an amount of preferably 0.01 to 100 mg, more preferably 0.1 to 50 mg per 1 kg of body weight. However, the dose of the above-mentioned triple extract is determined by considering various factors such as the route of administration and the number of treatments of the pharmaceutical composition as well as the patient's age, body weight, health condition, sex, severity of disease, diet and excretion rate In view of this point, one of ordinary skill in the art will be able to determine the appropriate effective dose according to the particular use as an anti-aging or anti-wrinkle treatment and treatment. The pharmaceutical composition according to the present invention is not particularly limited to the formulation, administration route and administration method as long as the effect of the present invention is exhibited.

As described above, the tubular extract of the present invention inhibits collagenase and promotes the production of collagen, so that it has excellent anti-aging activity. In particular, it has excellent wrinkle reduction, prevention or therapeutic effect, Can be used as an effective component of the composition.

FIG. 1 shows the results of measuring the effect of the extract of the present invention on the expression of collagenase-1 (A: Collagenase-1 protein inhibitory activity, B: Collagenase-1 mRNA expression inhibitory activity).
Figure 2 shows the results of measuring the effect of the tubercle extract of the present invention on the generation of type I collagen.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Triptych  Preparation of extract

<1-1> Preparation of ethanol extract of Samgyet roots

The dried triangular root was pulverized with a mixer, and 100 g of the pulverized triangular root sample was added to 1 L of ethanol and extracted with stirring at 50 캜 for 60 minutes. The extracted sample was filtered with filter paper of Whatman No. 2, and the filtered extract was concentrated with a vacuum rotary condenser to remove solvent components to obtain a triple rooted ethanol extract.

<1-2> Preparation of hot water extract of Samchab root

The dried triangular root was pulverized with a mixer, and 100 g of the pulverized triple root sample was added to 1 L of water and extracted with stirring at 100 ° C for 4 hours. The extracted samples were filtered with Wattmann filter paper No. 2, and the filtered extract was concentrated with a vacuum rotary condenser to remove the solvent components, thereby obtaining a watery extract of Samchab root.

&Lt; 1-3 > Preparation of Methanol Extract of Samjip Root

The dried triangular root was pulverized with a mixer, and 100 g of the pulverized triple root sample was added to 1 L of methanol and extracted with stirring at 50 ° C for 60 minutes. The extracted sample was filtered with Wattman No. 2 filter paper, and the filtered extract was concentrated with a vacuum rotary condenser to remove the solvent component to obtain a methanol extract of Trichotomous root.

&Lt; 1-4 > Preparation of triple rooted hexane extract

The dried triangular root was pulverized with a mixer, and 100 g of the pulverized triangular root sample was added to 1 L of nucleic acid and extracted with stirring at 50 ° C for 60 minutes. The extracted sample was filtered with a filter paper of Wattman No. 2, and the filtered extract was concentrated with a vacuum rotary condenser to remove solvent components to obtain a triple rooted hexane extract.

&Lt; 1-5 > Preparation of triacylglycerol ethyl acetate extract

The dried triangular root was pulverized with a mixer, and 100 g of the pulverized triangular root sample was added to 1 L of ethyl acetate and extracted with stirring at 50 캜 for 60 minutes. The extracted sample was filtered with a filter paper No. 2 of Wattman, and the filtered extract was concentrated with a vacuum rotary condenser to remove solvent components to obtain a triple root ethyl acetate extract.

<1-6> Preparation of chloroform-extracted triplex root

      The dried rhizome root root was crushed by a mixer, and 100 g of the crushed triquetrum root sample was added to 1 L of chloroform and extracted with stirring at 50 ° C for 60 minutes. The extracted sample

Was filtered with a filter paper No. 2 of Wattman, and the filtered extract was concentrated with a vacuum rotary condenser to remove solvent components to obtain a triple root chloroform extract.

<1-7> Preparation of Ethanol Extract of Triangular Leaves

The dried triangular leaf was pulverized with a mixer, and 100 g of the pulverized triangular leaf sample was added to 1 L of ethanol and extracted with stirring at 50 ° C for 60 minutes. The extracted sample was filtered through filter paper of Whatman No. 2, and the filtered extract was concentrated with a vacuum rotary condenser to remove solvent components to obtain a triangular leaf ethanol extract.

Triptych  Collagenase-1 inhibitory effect of extract

      The collagenase-1 inhibitory activity of the trihydrate ethanol extract prepared in Example 1-1 was measured by Western blot and RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction) method.

   Specifically, the protein was extracted from the fibroblast culture and protein was quantified using protein assay reagent (Bio-Rad Laboratories Inc., Hercules, Calif., USA). For Western blotting, the same amount of protein was heated for 5 minutes, cooled, and then transferred to nitrocellulose membrane (Amersham International, Little Chalfont, UK) by electrophoresis on 10% SDS-PAGE. The membrane was saturated with TBST (10 mM Tris, pH 7.5, 100 mM NaCl, 0.1% Tween 20) with 5% skimmed milk and blotted with primary antibody (1: 1000 dilution) Washed, blotted with secondary antibody (1: 2000 dilution) for 2 hours, and then washed 3 times with TBST. The blotted antibodies were analyzed with an ECL detection system (Amersham International, Little Chalfont, UK).

      Total RNA was isolated using TRIZOL (Invitrogen, USA) for the RT-PCR, and the total RNA was quantitated using primers of collagenase-1 and Taq Polymerase. Specifically, RT-PCR was performed using collagenase-1 primer at 18 ° C for 30 seconds at 94 ° C, 1 minute at 50 ° C, and 1 minute at 72 ° C. After the above procedure, electrophoresis was performed using 1% agarose gel, and the amount of mRNA expression of collagenase-1 was analyzed through the emission of EtBr (Ethidium bromide).

      As shown in FIG. 1, it was confirmed that the amount of collagenase-1 protein and mRNA expression was inhibited in a concentration-dependent manner upon treatment of the tubercle leaf extract of Example 1-1 (see FIG. 1).

From the above results, it can be seen that the tubular extract of Example 1-1 inhibits the expression of collagenolytic enzyme and thus can be effectively used for anti-aging or wrinkle reduction.

Triptych  Effect of collagen synthesis of type I extract

      The results of measuring the effect of type 1 collagen synthesis of the triacyl ethanol extract prepared in Example 1-1 according to the method of RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction) are shown in FIG.

      Total RNA was isolated from the fibroblasts obtained by RT-PCR using TRIZOL (Invitrogen, USA), and the total RNAs isolated were quantified and primers of type 1 collagen and Taq Polymerase were used. Specifically, RT-PCR was performed by repeating 30 times at 94 ° C for 30 seconds, 50 ° C for 1 minute, and 72 ° C for 1 minute using a type 1 collagen primer. After the above procedure, electrophoresis was carried out using 1% agarose gel, and the amount of type 1 collagen mRNA expression was analyzed through the emission of EtBr (Ethidium bromide).

      As shown in FIG. 2, it was confirmed that the amount of type 1 collagen mRNA expression was increased in a concentration-dependent manner upon treatment with the above-mentioned triple extract (see FIG. 2).

As a result, it can be seen that the triple extract of Example 1-1 can be effectively used for anti-aging or wrinkle improvement by increasing collagen production.

<Formulation Example 1 - Cosmetic>

      <1-1> Nourishing lotion (milk lotion)

The nutritional lotion was prepared according to a conventional method according to the ratio of the nutritional lotion formulation shown in the following Table 1 to the tubular extract of Example 1-1.

Compounding ingredient Formulation Example 1-1
(weight%) Triple extract 2.0 Squalane 5.0 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water to 100

      <1-2> Flexible longevity (skin lotion)

The softened longevity was prepared according to a conventional method according to the ratio of the softened longevity of the tablets of Example 1-1,

Compounding ingredient Formulation Example 1-2
weight(%) Triple extract 2.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 PEG 12 nonyl phenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water to 100

      <1-3> Nourishing cream

The nutritional cream was prepared according to the conventional method according to the formulation ratio of the nutritional cream shown in Table 3 below.

Compounding ingredient Formulation Example 1-3
(weight %) Triple extract 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 PEG60 hardened castor oil 2.0 Liquid paraffin 10 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 antiseptic Suitable amount Pigment Suitable amount Spices Suitable amount Purified water to 100

      <1-4> Massage Cream

Massage cream was prepared according to a conventional method according to the ratio of the massaging cream of the example 1 to the mass of the water extract of the example 3. [

Compounding ingredient Formulation Examples 1-4
(weight %) Triple extract 1.0 Wax 10.0 Polysorbate 60 1.5 PEG 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water to 100

      <1-5> Pack

The packs were prepared according to a conventional method according to the pack formulation ratio shown in Table 5 below.

Compounding ingredient Formulation Examples 1-5
(weight %) Triple extract 1.0 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 PEG 12 nonyl phenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Suitable amount Purified water to 100

      <1-6> Gel

The gel was prepared according to a conventional method according to the ratio of the gel formulations of the following Table 6 to the above-described three-quail extract of Example 1-1.

Compounding ingredient
Formulation Example 1-6
(weight %) Triple extract 0.5 Ethylenediamine sodium acetate 0.05 glycerin 5.0 Carboxyvinyl polymer 0.3 ethanol 5.0 PEG 60 hardened castor oil 0.5 Triethanolamine 0.3 Preservative, coloring, fragrance Suitable amount Purified water to 100

&Lt; Formulation Example 2 - Food &

      <2-1> Products of health food

1000 mg of the tubular extract of Example 1-1, 70 ug of vitamin A acetate, 1.0 mg of vitamin E, 0.13 mg of vitamin B1, 0.15 mg of vitamin B2, 0.5 mg of vitamin B6, 0.2 ug of vitamin B12, 10 mg of vitamin C, 10 ug of biotin, 1.7 mg of nicotinic acid amide, 50 ug of folate, 0.5 mg of calcium pantothenate, 1.75 mg of ferrous sulfate, 0.82 mg of zinc oxide, 25.3 mg of magnesium carbonate, 15 mg of monobasic potassium phosphate, 55 mg of calcium phosphate, Potassium 90 mg, calcium carbonate 100 mg, and magnesium chloride 24.8 mg. The compounding ratio may be arbitrarily changed. The above components are mixed according to a conventional method for producing healthy foods, and then granules And can be used for manufacturing a health food composition according to a conventional method.

      <2-2> Manufacture of health drinks

Purified water was added to 1000 mg of the tubular extract of Example 1-1, 1000 mg of citric acid, 100 g of oligosaccharide, 2 g of the plum concentrate and 1 g of taurine, and the above components were mixed After stirring for about 1 hour at 85 ° C, the resulting solution is filtered and sterilized in a sterile 2 L container, which can then be stored in a refrigerator for use in the manufacture of a health beverage composition.

      <2-3> Chewing gum

Chewing gum was prepared in a conventional manner by mixing 20% by weight of a gum base, 76.9% by weight of sugar, 1% by weight of a flavor and 2% by weight of water and 0.1% by weight of a tuberous extract of Example 1-1.

      <2-4> Candy

Candy was prepared by mixing 60% by weight of sugar, 39.8% by weight of starch syrup, 0.1% by weight of fragrance and 0.1% by weight of the above-mentioned tubular extract of Example 1-1.

      <2-5> Biscuit

A mixture of 0.75% by weight of sodium chloride, 0.78% by weight of glucose, 11.78% by weight of palm shortening, 1.54% by weight of ammonium, 0.17% by weight of sodium bicarbonate and 0.16% by weight of sodium bisulfite, 25.59% by weight of Grade I, 22.22% 1.45 wt% of rice flour, 0.0001 wt% of vitamin B, 0.0001 wt% of vitamin B, 0.04 wt% of milk fractions, 20.6998 wt% of water, 1.16 wt% of whole milk powder, 0.29 wt% 0.29% by weight of spray salt and 7.27% by weight of spray oil and the weight% of the tubular extract of Example 1-1 were blended to prepare biscuits by a conventional method.

&Lt; Formulation Example 3 - Medicines &

      <3-1> Powder

50 mg of the tubular extract of Example 1-1 and 2 g of crystalline cellulose were mixed and filled in an airtight container according to a conventional acid preparation method to prepare a powder.

      <3-2> Purification

The tablets were prepared by mixing 50 mg of the tubular extract of Example 1-1, 400 mg of crystalline cellulose, and 5 mg of magnesium stearate, followed by tableting according to a conventional preparation method.

      &Lt; 3-3 >

After mixing 30 mg of the tubercle extract of Example 1-1, 100 mg of whey protein, 400 mg of crystalline cellulose and 6 mg of magnesium stearate, the mixture was filled in gelatin capsules according to the conventional preparation method of capsules to prepare capsules .

The triple extract of the present invention inhibits collagen decomposition and promotes collagen synthesis, and thus has excellent anti-aging activity. In particular, it can be used as a cosmetic composition, a food composition or a pharmaceutical composition, There is a high possibility.

Claims (5)

Allium hookeri ) extract of the present invention.
Allium hookeri ) extract of the present invention.
Allium hookeri ) extract of the present invention.
The composition according to any one of claims 1 to 3, wherein the tubular extract is an extract of at least one solvent selected from the group consisting of water, organic solvents having 1 to 6 carbon atoms, and subcritical or supercritical fluids.
The method of claim 4, wherein the organic solvent having 1 to 6 carbon atoms is selected from the group consisting of alcohol having 1 to 6 carbon atoms, acetone, ether, benzene, chloroform, ethyl acetate Wherein the composition is selected from the group consisting of methylene chloride, hexane, cyclohexane, and petroleum ether.

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