KR20150064393A - The method of manufacturing the yeast which contains the composite for strengthening immunity and aids growth and development, and the health food including it - Google Patents

Abstract

The present invention relates to a composition which is produced by culturing microbial cells of yeasts belong to the genus Saccharomyomyces, and drying and powdering the same. The composition contains, with respect to the total weight of 21 g in an assay, 0.4 g or more of protein and 100 ug or more of thiamine (thiamine hydrochloride, C_12H_17 ClN_4OS, HCL:333.27), and so that the yeasts can be used as a mineral element related to intestinal regulation and lack of vitamin B groups, thus the beer yeasts can be also called as Brewer′s dried yeasts.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for preparing a composition for enhancing growth and development of a yeast,

The present invention is intended to produce yeast simultaneously with mineral components, amino acids and vitamins.

In the past, these manufacturing methods have been separately prepared and mixed. However, it is a manufacturing method in which minerals, protein components and vitamins can be synthesized by different manufacturing methods from the beginning.

The present invention is to obtain nutrients (amino acids, minerals, vitamins) necessary for growth period, indigestion due to nutritional imbalance in old age, malnutrition, intestinal fermentation, constipation, diarrhea and immunity enhancement.

When Saccharomyces cerevisae is cultured in a yeast that we want to use, we add malt juice to multiply the fermentation to obtain amino acids, minerals and vitamins

General yeast is produced for baking, alcohol, and livestock feed, but the intention of the present invention is to obtain raw materials for health functional foods, pharmaceutical raw materials and health foods.

Yeast separator - washing vessel - cooler - reservoir - dryer - product (edible yeast) - culture vessel - primary culture vessel - secondary culture vessel - main culture vessel (cooler, air cleaning device, blower)

It is a microorganism that fermentes sugar to produce C ₂ H ₅ , OH and CO _{2. It} is mainly used to produce alcohol such as alcohol yeast, brewer's yeast, and sake yeast. Yeast also gives aroma. Saccharomyces cerevisae We want to use it.

Molasses is used as a raw material and phosphoric acid and ammonium phosphate are added as a nitrogen (N) source to phosphates and malt juice, and the pH is adjusted to 4.0 ~ 4.5 and yeast is cultured at 25 ~ 30 ℃.

The addition of malt juice is due to the presence of Bios that promotes fermentation.

When culturing, dilute sugar solution (3 ~ 4%) and blow a large amount of air, cell growth of yeast becomes active and does not produce much alcohol.

Without air, most of the sugars are converted into alcohol and CO ₂ , and the yield of yeast is low.

On the other hand, the organic method refers to a method of culturing a yeast strain while adding diluted sugar solution and inorganic salt as the yeast is proliferated. After culturing for 8 ~ 12 hours, isolate the yeast cream state with yeast separator. Compressed yeast is obtained by compression, filtration and molding in a separator. It is refrigerated at 5 ° C or below or dried at low temperature to make dry yeast with storage stability

Appearance: This product is pale yellowish white brown powder with unusual smell and taste.

Identification: This preparation is a single or ovoid single cell with a diameter of about 6-12 μm (unit meta) when viewed under a microscope.

Purity test (decay) This product has no odor or taste that is unpleasant or altered.

Starch This product is only slightly visible even if it can not be seen or seen in the pale purple stain when iodine solution is added.

Loss on drying: Not more than 8.0% (1 g at 100 ° C for 8 hours)

Ash: Not more than 9.0% (Tested according to the batch of 1g drug test method)

Quantification method

Protein 50 mg of the preparation is precisely weighed and tested according to the nitrogen determination method.

1 ml of diluted hydrochloric acid and 80 ml of diluted hydrochloric acid are weighed precisely, and heated in a water bath at 80 to 85 ° C for 30 minutes while occasionally shaking. After cooling, add water to make exactly 100 ml and centrifuge for 10 minutes.

Take exactly 4 ml of the supernatant, accurately add 5 ml of acetic acid, sodium acetate TS and 1 ml of enzyme solution, and allow to stand at 45 to 50 ° C for 3 hours.

Take exactly 20 mL of this solution and add 2.5 mL of a Kromatograph column (a weakly acidic CM-crosslinked cellulose cation exchanger (H type) of 40-110 μm into a Kramatograph tube with an inner diameter of about 1 cm and a height of 17 cm) And allowed to flow out at a rate of about 0.5 ml per minute. Next, wash the inner wall of the kramatograph with a small amount of distilled water, and wash the kramatograph column at a rate of about 1 ml with 10 ml of the water for 1 minute.

This procedure is repeated twice.

Next, elute the test solution twice with 2.5 ml of diluted phosphoric acid (1 to 50) at a rate of about 0.5 ml per minute for 1 minute.

Add exactly 1 ml of the standard solution in the test solution, add 10 mg of sodium 1-octasulfate and dissolve it. Use this solution as the sample solution.

Separately, standard samples of hydrochloric acid (measured in the same way as hydrochloric acid,

Approximately 15 mg is precisely weighed and dissolved in 0.001 mol / l hydrochloric acid solution to make exactly 100 ml.

Take exactly 1 ml of this solution and add mobile phase to make exactly 100 ml.

 Take exactly 1 ml of this solution, accurately add 1 ml of the internal standard solution, add 3 ml of the mobile phase, and use this solution as the standard solution.

200 μl each of the sample solution and the standard solution is tested according to the liquid chromatographic method under the following conditions.

Calculate the peak area ratio QT and QS of the internal standard material.

The amount (μg) of thiamine in 1 g of this preparation

= Amount of standard product of hydrochloric acid thiamine converted to anhydride (mg)

The amount of protein (mg)

10 mg of phenacetin as an internal standard solution is dissolved in acetonitrile to make 100 ml. Dilute acetonitrile (1 to 5) in 1 ml of this solution to make 100 ml.

Detector: Ultraviolet absorbance (measuring wavelength 254 nm)

Column: A stainless steel tube having an internal diameter of 4 mm and a length of 15 to 30 cm is filled with 5 to 10 탆 of octadecylsilicone silica gel for liquid chromatography.

Column temperature: Maintain a constant temperature around 40 캜

Mobile phase: 2.7 g of potassium dihydrogenphosphate is dissolved in 1000 ml of water, and diluted phosphoric acid (1-10) is added to adjust pH to 3.5. 1.6 g of 1-octasulfone sodium is dissolved in 800 ml of this solution, and 200 ml of acetonitrile is added

Flow rate: Write that the retention time of the teething spills out in order of the material and the separation degree is 8 or more

Ingredients: Essence is minerals, amino acids, vitamins, dry yeast

The content of dry brewer's yeast is 46.7 ~ 57% crude protein, 7.94 ~ 9.04% inorganic, 2.20 ~ 6.68% dry weight, 107 ~ 151mg / g thiamine, 28 ~ 36mg / g riboflavin and 411 ~ 445mg / g nicotinic acid .

18 kinds of amino acids (dried beer yeast) supplied to health food

Amino acids Amino acids (mg / 100g) among

Lysine, Methionine, Tryptophan, Valine, Leucine, Isoleucine, Threonine, Phenylalanine, Histidine, Cystine, Asparticacid, Serine, Glutaminc acid, Proline, Glycine, Tyrosine, Arginine, Alanine,

It contains amino acids and protein amino acid composition is similar to animal protein.

Minerals (mg / 100g)

(Ca), potassium (K), phosphorus (P), magnesium (Mg), sodium (Na), manganese, copper, cobalt and zinc

12 vitamins (Vitamins (mg / 100g)

Vitamin B _1, Vitamin B _2, Vitamin B _5, Vitamin B _6, Biotin, Vitamin B _12, Nicotinamide (pp), Folic acid, Vitamin C, Vitamin E, Niacin,

It is a vitamin source in that it contains the above-mentioned ingredients, and in particular, it is biologically active. Therefore, it has growth and immunity, and contains many useful ingredients such as ergosterine, hexane and glutathione.

All of the ingredients containing the helping action are synthesized to help nutrition, metabolism, growth and development, immune function, food promotion, and dressing.

This patent application focuses on three points called minerals, which are necessary for enhancing the immunity of the vitamin B complex (especially B ₁ ) and protein (amino acid).

Claims (3)

Saccharomyces sp. Cells were added to the primary culture tube, and after 5 hours,
The second step of incubating the primary cultured cells for 5 hours in the secondary culture tube The culture solution obtained in claim 2 is passed through a cooler, an air cleaning device, a third step of discharging the culture gas through a blower,
After the third step, the product is defatted through a deaerator, and then the product is subjected to a fourth step, a fifth step, which is manufactured through a washing container separating baking, health food, and edible yeast from the yeast separator The process of separating the baking dish and the edible yeast from the refiner was carried out for 3 hours,
The 7th step of establishing the property for 1 hour through the molding machine,
Store in refrigerator 4 ~ 0 ℃ and use for baking 8th step,
Process of 1 ~ 8 steps, Separation with edible yeast, Process of drying in a dryer through a cooler reservoir at 40 ℃ for 2 hours and use as edible yeast.

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