KR20150051631A - Composition comprising extract of black rice as an effective component for prevention or treatment of metabolic bone disease - Google Patents

Abstract

The present invention relates to a composition containing an extract of black rice as an active ingredient for preventing or treating metabolic bone diseases such as osteoporosis, bone formation disorder, fracture, or the like. A reed extract of the present invention promotes the expression of ALP, osterix, and RUNX2, which are related to the differentiation of osteoblasts, and increases a bone mineral density (BMD) and a bone strength in an ovariectomized osteoporosis animal model, and accordingly, can be used as a medicinal product or a health functional food which is useful for metabolic bone diseases.

Description

[0001] The present invention relates to a composition for prevention or treatment of bone metabolic diseases comprising black rice extract as an active ingredient,

The present invention relates to a composition for prevention and treatment of bone metabolic diseases comprising an extract of black rice, and the composition of the present invention can be applied to the production of health functional foods and medicines for the prevention and treatment of osteoporosis.

Osteoporosis refers to a skeletal disorder in which the risk of fracture and bone fragility are increased due to low bone mass and bone destruction. Osteoporosis is a particularly frequent occurrence in postmenopausal women, a disease in which the amount of bone is markedly reduced by the decreased secretion of estrogen. The decrease in the amount of bone may be due to individual differences or various other causes, but if the amount of bone excessively decreases and falls below a predetermined value, fracture easily occurs even in a small impact. Osteoporosis can not lead to a healthy life by restricting long-term activities due to various fractures caused by weakening of the bones, especially femur fracture or vertebral fracture, rather than the symptom itself, resulting in 15% of the elderly death It is known.

Osteodystrophy is also called bone dystrophy and is a bone disease caused by chronic renal failure. It is born by abnormally abnormal kidney function, and when the kidney is weak, it does not dialyse. This kind of bone disease is called Renal Osteodystrophy. Bone diseases associated with osteophagia include osteomalacia fibrous osteitis (osteitis fibrosa).

On the other hand, black rice ( Oryza sative L ) is native to Thailand and has been cultivated in Jindo since 1991 in Korea. Black rice contains a large amount of selenium, and it has cancer prevention effect. There is a recent report in China that 40 patients with rectal cancer were treated with black rice for a prolonged period of time and the condition improved.

In 1998, professor Hwang Tae-Ik of the agriculture department of Chonnam National University also reported that the black rice has excellent efficacy in cancer cell removal and gastric ulcer treatment. In addition, vitamin B and vitamin E in black rice contains more than four times as much as ordinary rice, and it is effective for prevention of diabetes and adult diseases as well as female beauty.

Recently, anthocyanins in black rice have been reported to inhibit fatty liver. However, the efficacy of osteoporosis and other metabolic diseases has not yet been revealed.

It is an object of the present invention to provide a composition for the prevention or treatment of bone metabolic diseases such as osteoporosis, osteoporosis or fracture derived from a natural product having no toxicity.

The composition for prevention or treatment of bone metabolic diseases of the present invention contains a black rice extract as an active ingredient.

As used herein, the term " comprising as an active ingredient " means an amount sufficient to achieve the efficacy or activity of the black rice extract.

The bone metabolic diseases of the present invention include osteoporosis, bone formation disorder or fracture.

In the present invention, 'osteoporosis' includes clinical classification according to the measurement of bone mineral density (BMD), ie, osteopenia, osteoporosis, and severe osteoporosis. It also includes primary osteoporosis type 1 (postmenopausal osteoporosis type 1), type 2 senile osteoporosis type 2 (secondary osteoporosis), and secondary osteoporosis.

In the present invention, 'osteodystrophy' includes osteomalacia, osteitis fibrosa and the like.

In the present invention, 'fracture' refers to a state in which the continuity of bone or cartilage is completely or incompletely lost or a linear deformation occurs. Fractures are pathologic fractures caused by osteoporosis and tumor osteomyelitis, which are anatomical location, extent of fracture, direction of fracture, presence of open window, number of fractures, stability, dislocation of fracture, And fatigue fractures caused by being applied, and the term "fracture" includes all of them.

The extract of the present invention may be an extract of purified water, water, an organic solvent or a mixed solvent thereof. Preferably, the organic solvent may be at least one solvent selected from the group consisting of alcohols having 1 to 4 carbon atoms, hexane, dichloromethane, ethyl acetate, acetone, chloroform and diethyl ether, more preferably methanol, ethanol or methanol And an aqueous solution of ethanol. In addition to the organic solvent, polar solvents such as acetic acid, dimethyl-formamide (DMFO) and dimethyl sulfoxide (DMSO), acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, 2,2,4-trimethylpentane, decane, Butene, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, Non-polar solvents such as benzene, diethyl ether, diethyl sulfide, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride and THF (Tetrahydrofuran) may be used.

The term 'extract' used in the present invention includes fractions obtained by further fractionating the above extract. That is, not only the water, the lower alcohol having 1 to 4 carbon atoms, the nonpolar solvent, or the mixed solvent thereof, but also the fractions fractionated by the above-mentioned solvents, or the ultrafiltration membrane having various molecular weight cutoff values And fractions obtained.

The method for producing the extract of the present invention can be carried out by, for example, drying and crushing black rice, and then adding water, an organic solvent or a mixture thereof to the dried sample in an amount of about 1 to 50 times, preferably about 10 to 40 times, Solvent extraction, hot water extraction, cold extraction, reflux cooling extraction, ultrasonic extraction or supercritical extraction at a temperature of 20 to 100 ° C, preferably 40 to 60 ° C for about 24 hours or for 2 to 4 hours The extract of the present invention can be obtained by using an extraction method, preferably extracting hot water, and then extracting the obtained extract by filtration, concentration under reduced pressure or drying.

The black rice extract of the present invention exhibits an osteoblast differentiation increasing activity and a bone mineral density increasing activity, and contains the black rice extract in an amount of 0.1 to 50% by weight based on the total weight of the composition. However, the composition is not limited thereto, and may vary depending on the condition of the patient, the type of disease, and the progress of the disease.

The composition for prevention or treatment of bone metabolic diseases including the black rice extract of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the production of pharmaceutical compositions.

The composition containing the extract according to the present invention may be formulated in the form of powders, granules, tablets, capsules, oral preparations such as suspensions, emulsions, syrups and aerosols, external preparations, suppositories and sterilized injection solutions, Can be used.

Examples of carriers, excipients and diluents that can be included in the composition containing the black rice extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, Calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose ), Lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The preferred dose of the black rice extract of the present invention varies depending on the condition and body weight of the patient, the degree of disease, the type of drug, the administration route and the period of time, but can be appropriately selected by the therapist. However, for the desired effect, the extract of the present invention is preferably administered at a dose of 0.01 mg / kg to 10 g / kg per day, preferably 1 mg / kg to 1 g / kg per day. The administration may be carried out once a day or divided into several doses. Accordingly, the dosage is not limited in any way to the scope of the present invention.

The composition of the present invention may be administered to mammals such as rats, mice, livestock, humans, and the like in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intracerebroventricular injections.

The present invention provides a health functional food for preventing or ameliorating bone metabolic diseases, comprising a black rice extract and a food acceptable food supplementary additive exhibiting osteoblast differentiation increasing activity and bone mineral density increasing activity.

The health functional food of the present invention includes various foods, gums, tea, vitamin complex, health supplement foods and the like, and can be used as powder, granule, tablet, capsule or beverage.

Since the reed extract of the present invention has little toxicity and side effects, it can be safely used for prolonged use even for prophylactic purposes.

When the black rice extract of the present invention is added to foods or beverages for the purpose of preventing or improving bone metabolic diseases, the amount of the extract in foods or beverages is generally from 0.01 to 15% by weight of the total food weight of the health food composition of the present invention And the health beverage composition may be added at a ratio of 0.02 to 10 g, preferably 0.3 to 1 g, based on 100 ml.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates such as ordinary beverages as an additional ingredient, as long as it contains the extract as an essential ingredient at the indicated ratio, and there is no particular limitation to the liquid ingredient. Examples of the above-mentioned natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like Sugar, and sugar alcohols such as xylitol, sorbitol, and erythritol. Natural flavors (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the health functional food of the present invention may contain flavorings such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and heavies (cheese, chocolate etc.), pectic acid and its salts, And salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the health functional foods of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not so important, but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the health functional food of the present invention.

The present invention relates to a composition for prevention or treatment of bone metabolic diseases such as osteoporosis, osteoporosis or fracture, which comprises black rice extract as an active ingredient. The black rice extract of the present invention contains ALP, osterix, RUNX2 (BMD) and bone strength in animal models of ovariectomized osteoporosis, and can be used as medicines or health functional foods useful for bone metabolism diseases.

FIG. 1 is a photograph of ALP staining of the C3H10T1 / 2 cell line in Experimental Example 1 after treatment with the black rice ethanol extract of Preparation Example 1 for 6 days in the osteogenic differentiation medium.
FIG. 2 is a graph showing the relative mRNA expression levels of genes related to osteoblast differentiation after treatment of the C3H10T1 / 2 cell line with the black rice ethanol extract of Preparation Example 1 in the osteoblast differentiation medium in Experimental Example 2. FIG.
FIG. 3 is a photograph of ALP staining of the MC3T3-E1 cell line in Experimental Example 3 after treatment with the black rice ethanol extract of Preparation Example 1 for 6 days in the osteogenic differentiation medium.
FIG. 4 is a graph showing the effect of the ovariectomized experimental group (OVX) on the ovariectomy (OVX) and the experimental group (OVX + BRE) FIG. In FIG. 4, * is P <0.05, ** is P <0.005, and *** is P <0.0005.
FIG. 5 is a graph showing the bone mineral density of each experimental group when rat was 19 weeks old in Experimental Example 4. FIG. In Fig. 5, * is P < 0.05, ** is P < 0.005, *** is P <
FIG. 6 is a graph showing the bone strength of each experimental group when the rat became 19 weeks old in Experimental Example 4. FIG.
FIG. 7 is a photograph of ALP staining of the C3H10T1 / 2 cell line in Experimental Example 5 after 6 days treatment with the black rice water extract of Production Example 2 and the black rice hexane extract of Production Example 3 in the osteoblast differentiation medium.
8 is a graph showing the relative mRNA expression levels of the genes involved in osteoblast differentiation after treatment of the C3H10T1 / 2 cell line in Experimental Example 6 with the black rice water extract of Preparation Example 2 and the black rice hexane extract of Production Example 3 for 6 days in the osteoblast differentiation medium Fig.
FIG. 9 is a graph showing the amount of ALP expression according to the differentiation progression time by treating the C3H10T1 / 2 cell line with the black rice water extract in Experimental Example 7.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following examples and experimental examples are illustrative of the present invention, and the present invention is not limited thereto.

Production Example 1: Preparation of black rice ethanol extract

The black rice used in the present invention was directly harvested at the National Institute of Food Science and Technology, Milyang Rural Development Administration, Gyeongsangnam-do. The harvested black rice grains were thoroughly dried and finely pulverized to obtain a powdery sample.

400 ml of a 75% by weight aqueous ethanol solution was added to 20 g of the powder sample, and the solution was firstly extracted at 120 rpm and 45 ° C. for 24 hours in a shaking incubator. The supernatant was filtered with a Whatman No.1 filter paper, 400 ml of a 75% by weight ethanol aqueous solution was added per 20 g of the sample, extracted again for 24 hours, and filtered in the same manner as described above. The filtrate was concentrated under reduced pressure at 45 DEG C with a rotary vacuum evaporator, and the solvent was completely blown off to obtain a 75 wt% ethanol extract of black rice.

Experimental Example 1. Measurement of ALP activity in C3H10T1 / 2 cells of black rice ethanol extract

The C3H10T1 / 2 cell line derived from mouse embryonic fibroblasts is a pluripotent stem cell line that can differentiate into various cell lines including osteoblasts. One of the characteristics of the osteoblast is that it exhibits ALP (Alkaline phosphatase) activity. Therefore, the osteoblast differentiation effect was measured by ALP activity of C3H10T1 / 2 cell lines.

The C3H10T1 / 2 cell line was cultured in DMEM medium supplemented with 10% FBS, 1% penicillin and streptomycin at 37 ° C in a 5% CO 2 environment. The C3H10T1 / 2 cells were cultured for 3 days in a 6-well plate with a medium containing 10 mM glycerophosphate and 50 μg / ml ascorbic acid for bone cell differentiation at a concentration of 2.5 × 10 4 / ml, 10 μg / ml, 20 μg / ml, 40 μg / ml and 80 μg / ml of black rice ethanol extract were added to the medium and the medium was changed every 3 days for 6 days.

A negative control (DMSO) was used in which DMSO alone was added without adding the black rice ethanol extract of Preparation Example 1, and the results of staining for confirming ALP activity according to the concentration of the black rice ethanol extract of Preparation Example 1 are shown in FIG.

In Fig. 1, it was confirmed that the black rice extract increased ALP activity in a concentration-dependent manner.

EXPERIMENTAL EXAMPLE 2 Measurement of osteoblast differentiation factor expression by real-time PCR of ethanol extract of black rice

In Experimental Example 1, C3H10T1 / 2 cells were differentiated for 6 days, and the amount of mRNA expression of ALP and Osterix, which are differentiation factors involved in osteoblast formation, was confirmed by real-time PCR and shown in FIG.

2, the amount of mRNA expression of ALP and osterix in the group treated with the black rice extract was significantly increased compared with the negative control (DMSO).

Experimental Example 3. Measurement of ALP activity in MC3T3-E1 cells of black rice ethanol extract

MC3T3-E1 cells are osteoblasts and can differentiate into osteoblasts when inducing differentiation through specific stimuli. The osteoblast differentiation effect was measured by ALP activity of MC3T3-E1 cells.

The MC3T3-E1 cell line was cultured in a 5% CO 2 environment at 37 ° C in α-MEM medium supplemented with 10% FBS, 1% penicillin and streptomycin. The MC3T3-E1 cells were cultured for 3 days in a 6-well plate with a medium containing 10 mM glycerophosphate and 50 μg / ml ascorbic acid for osteoclast differentiation at a concentration of 2.5 × 10 4 / ml. 20 μg / ml, 40 μg / ml and 80 μg / ml of black rice ethanol extract were added to the medium, and the medium was changed every 3 days and differentiated for 6 days.

A negative control (DMSO) in which only DMSO was added without adding the black rice ethanol extract of Preparation Example 1 was used, and in the MC3T3-E1 cell of FIG. 3, as in the C3H10T1 / 2 cell of FIG. 1, the ALP activity was increased in a concentration- Respectively.

Experimental Example 4: ovariectomy of black rice ethanol extract (ovariectomy) Test using an animal model of osteoporosis

To examine the effect of the ethanol extract of black rice (Et-OH) of Preparation Example 1 on the treatment and prevention of osteoporosis, bone mineral density was measured and histological analysis was performed after administration of the extract to ovariectomized rats.

(1) Animal breeding and ovariectomy

Ten-week-old female SD rats were purchased from Orient Biotech and had a purifying period of one week. At 11 weeks of age, ovariectomies were performed and he had a one week recovery period. Experimental animals were housed in a single cage. The environmental conditions were adjusted to room temperature 24 ± 2 ℃, relative humidity 55 ± 10%, and illumination time 12 hours. Feed and water were freely available.

The experimental animals were divided into three groups. Ten experimental ovariectomized group (SHAM), 10 ovariectomized group (OVX), 10 ovariectomized group, experimental group treated with ethanol extract of black rice in preparation 1 after ovariectomy Ovx + BRE 100 mg / kg). Ovariectomy was performed by using anesthesia with Zoletil and rumpun, and then depilating and incising the skin, removing the ovaries and suturing. The test samples were orally administered for a total of 8 weeks.

As a result, the ovariectomy group (OVX) showed a tendency to increase in body weight compared with the ovariectomized group (SHAM) due to the reduction of oestrogen secretion due to ovariectomy, and the ovariectomized brown rice ethanol extract (OVX + BRE 100 mg / kg) showed a tendency to lose weight but no significant difference compared to the ovariectomized group (OVX) (Fig. 4).

(2) Measurement of BMD

When the animal was 19 weeks of age, the femur and tibia were separated and the femur was used to measure bone density. BMD was measured using pDEXA (Forearm: X-Ray, NORLAND, Bone Densitometer, USA).

As a result, the BMD of the ovariectomized group (OVX) was significantly lower than that of the ovariectomized group (SHAM), and the ovariectomized group (BMD) (BMD) of ovariectomized rats (OVX + BRE 100 mg / kg) was significantly increased compared to ovariectomized group (OVX) (FIG. 5).

(3) Measurement of bone strength (BS)

The right femur was extracted from the ovariectomized group (SHAM), the ovariectomized group (OVX), and the ovariectomized group (Ovx + BRE) , That is, the breaking force (kg).

As a result, the bone strength (BS) of the ovariectomized group (OVX) was not significantly different from that of the ovariectomized group (SHAM) but decreased to some extent, and the ovariectomized brown rice ethanol extract of Preparation Example 1 The bone strength (BS) of the experimental group (OVX + BRE 100 mg / kg) was significantly increased compared to the ovariectomized group (OVX) (FIG. 6).

Production Example 2: Preparation of reed water extract

The same procedure as in Preparation Example 1 was carried out except that the extraction solvent was replaced with 75% by weight aqueous ethanol solution to prepare a black rice water extract.

Production Example 3: Preparation of reed hexane extract

The same procedure as in Preparation Example 1 was carried out except that the extraction solvent was changed to hexane (n-hexane) instead of 75 wt% aqueous ethanol solution to prepare a black rice hexane extract.

Experimental Example 5. Measurement of ALP activity in C3H10T1 / 2 cells of black rice water extract and hexane extract

The C3H10T1 / 2 cell line was cultured in the same manner as in Experimental Example 1, except that the black rice water extract and the black rice hexane extract were added at a concentration of 40 占 퐂 / ml and 80 占 퐂 / ml instead of the black rice ethanol extract of Preparation Example 1, , And the ALP activity was measured and shown in FIG.

7, it was confirmed that ALP activity was significantly higher in the black rice water extract than in the black rice hexane extract.

Experimental Example 6 Measurement of Osteoblast Differentiation Factor Expression by Real-time PCR of Black Water Extract and Hexane Extract

After the C3H10T1 / 2 cells were differentiated for 6 days in Experimental Example 5, mRNA expression levels of ALP, Osterix, OPN and Runx2, which are differentiation factors involved in osteoblast formation, were confirmed by Real-time PCR, Respectively.

In FIG. 8, mRNA expression levels of ALP, Osterix, OPN and Runx2 in the group treated with the black rice water extract were found to be higher than those of the negative control group (DMSO). In the case of the black rice hexane extract, only the expression amount of Osterix mRNA was increased Respectively.

EXPERIMENTAL EXAMPLE 7 Measurement of Osteoblast Differentiation Factor Expression by Real-time PCR over Time in Differentiation Process of Black Water Extract

In Experimental Example 5, C3H10T1 / 2 cells were differentiated for 6 days by adding 80 μg / ml of black rice water extract, and the mRNA expression level of ALP, which is a differentiation factor involved in osteoblast formation, was measured by real- The change was confirmed and is shown in Fig.

In the case of negative control in FIG. 9, there was no change in the progression of differentiation, but in the case of BRDW, the expression level of ALP was continuously increased as the differentiation progressed from the first day of differentiation.

Hereinafter, formulation examples of the composition containing the extract of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Preparation Example 1. Preparation of powder

20 mg of the black rice ethanol extract of Preparation Example 1

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

Formulation Example 2. Preparation of tablets

10 mg of the black rice ethanol extract of Preparation Example 1

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

Formulation Example 3. Preparation of capsules

10 mg of the black rice ethanol extract of Preparation Example 1

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

Formulation Example 4. Preparation of injection

10 mg of the black rice ethanol extract of Preparation Example 1

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO _4, 12H ₂ O 26 mg

(2 ml) per 1 ampoule according to the usual injection preparation method.

Formulation Example 5. Preparation of a liquid preparation

20 mg of the black rice ethanol extract of Preparation Example 1

10 g per isomer

5 g mannitol

Purified water quantity

Each component was added to purified water in accordance with the usual liquid preparation method and dissolved, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was adjusted to 100 ml with purified water, And sterilized to prepare a liquid preparation.

Formulation Example 6. Preparation of Healthy Foods

1,000 mg of the black rice ethanol extract of Preparation Example 1

Vitamin mixture quantity

70 [mu] g of vitamin A acetate

Vitamin E 1.0 mg

0.13 mg vitamin B1

0.15 mg of vitamin B2

0.5 mg vitamin B6

0.2 [mu] g vitamin B12

10 mg vitamin C

Biotin 10 μg

Nicotinic acid amide 1.7 mg

50 ㎍ of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

15 mg of potassium phosphate monobasic

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

24.8 mg of magnesium chloride

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Formulation Example 7. Preparation of health drink

1,000 mg of the black rice ethanol extract of Preparation Example 1

Citric acid 1,000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added to a total of 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated for about 1 hour at 85 DEG C with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, &Lt; / RTI &gt;

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

Claims (7)

A composition for preventing or treating bone metabolic diseases, which contains black rice extract as an active ingredient.
The composition for preventing and treating bone metabolic diseases according to claim 1, wherein the bone metabolic disease is osteoporosis, bone formation disorder or fracture.
The composition for prevention and treatment of bone metabolic diseases according to claim 1, wherein the black rice extract is an extract of water, an organic solvent or a mixed solvent thereof.
The composition for preventing and treating bone metabolic diseases according to claim 3, wherein the reed extract is a fraction obtained by fractionating the extract with water, an organic solvent or a mixed solvent thereof.
The method according to claim 3 or 4, wherein the organic solvent is at least one solvent selected from the group consisting of alcohols having 1 to 4 carbon atoms, hexane, dichloromethane, ethyl acetate, acetone, chloroform and diethyl ether A composition for prevention and treatment of bone metabolic diseases.
[Claim 4] The method according to claim 3, wherein the extract is obtained by stirring, extracting with hot water, refluxing, refluxing cooling, ultrasonic extraction, or supercritical extraction using an extraction solvent of 1 to 50 times the weight of dried black rice A composition for prevention and treatment of bone metabolic diseases.
A health functional food for prevention or improvement of bone metabolism diseases containing black rice extract as an active ingredient.

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